| cloning and characterization of a glutamine transport operon of bacillus stearothermophilus nub36: effect of temperature on regulation of transcription. | we cloned and sequenced a fragment of the bacillus stearothermophilus nub36 chromosome that contains two open reading frames (orfs) whose products were detected only in cells of cultures grown in complex medium at high temperature. the nucleotide sequence of the two orfs exhibited significant identity to the sequence of the glnq and glnh loci of the glutamine transport system in enteric bacteria. in addition, growth response to glutamine, sensitivity to the toxic glutamine analog gamma-l-glutamy ... | 1991 | 1856180 |
| temperature-induced protein synthesis in bacillus stearothermophilus nub36. | cultures of bacillus stearothermophilus subjected to a temperature shift-up or shift-down of 15 degrees c within the normal temperature range of growth (45 to 65 degrees c) enter a transient adaptation period before exponential growth at the new temperature. the de novo synthesis of some proteins coincides with the adaptation period. | 1991 | 1856181 |
| cloning, overexpression, purification and crystallisation of ribosomal protein l9 from bacillus stearothermophilus. | the cloning, sequencing and overexpression of the gene coding for bacillus stearothermophilus ribosomal protein l9 is described. the sequence corresponds directly to that presented for the protein itself by classical methods, differing at only a few amino acid positions. the purification and crystallisation of the corresponding l9 protein is presented. the crystals are isomorphous to those described for l9 obtained by conventional methods. | 1991 | 1864369 |
| cloning the bstvi restriction-modification system in escherichia coli. | a standard dna modification methyltransferase (mtase) selection protocol was followed to clone the bstvi restriction and modification system from bacillus stearothermophilus in escherichia coli. both genes were contained in a 4.4-kb ecori fragment from b. stearothermophilus v chromosomal dna. the heterologous expression of these genes did not depend on their orientation in the vector, suggesting that the genes are expressed in e. coli under the control of promoters located on the cloned fragment ... | 1991 | 1864512 |
| molecular dissection of translation initiation factor if2. evidence for two structural and functional domains. | by means of limited proteolysis of bacillus stearothermophilus initiation factor if2 and genetic manipulation of its structural gene, infb, we have been able to produce (or hyperproduce) and purify two polypeptide fragments corresponding to two structurally and functionally separate domains of the protein. the first is the g-domain (approximately 41 kda), which makes up the central part of the molecule and contains the conserved structural elements found in all gtp/gdp-binding sites of g-protein ... | 1991 | 1885570 |
| conservation and evolution of the nucleus-encoded and chloroplast-specific ribosomal proteins in pea and spinach. | two cdna clones have been isolated from a lambda g11 cdna library constructed with poly(a)+ mrnas prepared from spinach seedlings. these nuclear cdnas encode chloroplast (cp) ribosomal (r) proteins designated l24 and l40. these r-proteins have been identified in the cp 50s r-subunit by immunoblot analysis, amino acid (aa) composition and n-terminal aa sequencing. the l24 r-protein contains a central eubacterial homologous core with the n- and c-terminal polypeptide extensions. the l40 r-protein ... | 1991 | 1889743 |
| physical studies on membrane lipids of bacillus stearothermophilus temperature and calcium effects. | bacillus stearothermophilus was grown at the optimal temperature range (center, 65 degrees c), below it (48 and 55 degrees c), and above it (68 degrees c), in a complex medium with or without 2.5 mm ca2+. the ca(2+)-supplement improves growth at sub- and supraoptimal temperatures and extends it to higher temperatures (jurado et al. (1987) j. gen. microbiol. 133, 507-513). the phospholipid composition of cultures obtained in the different growth conditions was studied. phosphatidylethanolamine wa ... | 1991 | 1898060 |
| cloning of a chromosomal alpha-amylase gene from bacillus stearothermophilus. | we have cloned and sequenced a gene for a heat-stable alpha-amylase from a natural isolate of bacillus stearothermophilus. previously, it had been shown that b. stearothermophilus amylase genes may be harboured on indigenous plasmids. we have found that our isolate harbours the amylase gene only on the chromosome and not on its indigenous plasmid. | 1991 | 1903751 |
| thermostable alanine racemase of bacillus stearothermophilus. construction and expression of active fragmentary enzyme. | limited proteolysis studies on alanine racemase suggested that the enzyme subunit is composed of two domains (galakatos, n. g., and walsh, c. t. (1987) biochemistry 26, 8475-8480). we have constructed a mutant gene that tandemly encodes the two polypeptides of the bacillus stearothermophilus enzyme subunit cleaved at the position corresponding to the predicted hinge region. the mutant gene product purified was shown to be composed of two sets of the two polypeptide fragments and was immunologica ... | 1991 | 1906880 |
| two lipoyl domains in the dihydrolipoamide acetyltransferase chain of the pyruvate dehydrogenase multienzyme complex of streptococcus faecalis. | a fragment of dna incorporating the gene, pdhc, that encodes the dihydrolipoamide acetyltransferase (e2) chain of the pyruvate dehydrogenase multienzyme complex of streptococcus faecalis was cloned and a dna sequence of 2360 bp was determined. the pdhc gene (1620 bp) corresponds to an e2 chain of 539 amino acid residues, mr 56,466, comprising two lipoyl domains, a peripheral subunit-binding domain and an acetyltransferase domain, linked together by regions of polypeptide chain rich in alanine, p ... | 1991 | 1908789 |
| sequence-specific 1h-nmr assignments and secondary structure of the lipoyl domain of the bacillus stearothermophilus pyruvate dehydrogenase multienzyme complex. | the lipoyl domain (residues 1-85) of the lipoate-acetyltransferase polypeptide chain of the pyruvate dehydrogenase multienzyme complex of bacillus stearothermophilus has been subjected to detailed structural analysis by means of two-dimensional (2d) 1h-nmr spectroscopy at 400 mhz. sequence-specific proton resonance assignments were made, but at this field strength not all of the side-chain protons could be assigned, especially from complex spin systems like those of leucine, proline and lysine r ... | 1991 | 1915365 |
| analysis of the active center of bacillus stearothermophilus neopullulanase. | the active center of the neopullulanase from bacillus stearothermophilus was analyzed by means of site-directed mutagenesis. the amino acid residues located in the active center of the neopullulanase were tentatively identified according to a molecular model of taka-amylase a and homology analysis of the amino acid sequences of neopullulanse, taka-amylase a, and other amylolytic enzymes. when amino acid residues glu and asp, corresponding to the putative catalytic sites, were replaced by the opp ... | 1991 | 1917847 |
| growth kinetics of bacillus stearothermophilus br219. | bacillus stearothermophilus br219, a phenol-resistant thermophile, can convert phenol to the specialty chemical catechol. the growth kinetics of this organism were studied in batch, continuous, and immobilized-cell culture. batch growth was insensitive to ph between 6.0 and 8.0, but little growth occurred at 5.5. in continuous culture on a dilute medium supplemented with 10 mm phenol, several steady states were achieved between dilution rates of 0.25 and 1.3 h-1. phenol degradation was found to ... | 1991 | 1929366 |
| influence of an s-layer on surface properties of bacillus stearothermophilus. | various aspects of surface properties of the s-layer-carrying bacillus stearothermophilus pv72 and of an s-layer-deficient mutant (strain pv72/t5) have been tested by adsorption assays on solid surfaces, electrostatic interaction chromatography and hydrophobic interaction chromatography. the adsorption assays have shown that cell adhesion of the s-layer-carrying strain was less influenced by environmental changes than it was with the s-layer-deficient mutant. electrostatic interaction chromatogr ... | 1991 | 1953302 |
| [the suitability of commercial bioindicators with spores of b. stearothermophilus for the testing of formaldehyde gas sterilizers]. | commercially available biological indicators with spores of b. stearothermophilus were investigated by the hygiene-institutes of kiel and lubeck. the objective was to find out if those indicators to which sheep blood was added subsequently correspond to the formaldehyde resistance required by. din 58948, part 14 (din 58948, part 13). both working groups determined unanimously that the indicators of one producer showed a resistance too low compared to the remaining biological indicators showing a ... | 1991 | 1953931 |
| bst dna polymerase permits rapid sequence analysis from nanogram amounts of template. | a simple, rapid method is presented for the enzymatic sequence analysis of nanogram amounts of single-stranded or double-stranded dna. this approach employs the thermostable dna polymerase from bacillus sterothermophilus and exploits its ability to efficiently extend all of the template-primer complex, even at low substrate concentrations. the procedure requires few pipetting steps, no preannealing step and very short reaction time. this method can significantly reduce the cost associated with d ... | 1991 | 1954022 |
| bacterial catalase-peroxidases are gene duplicated members of the plant peroxidase superfamily. | bacterial catalase-peroxidases are enzymes containing 0.5-1.0 heme per subunit. the identical subunits are generally 80 kda in size, and the sequenced subunits of e. coli, s. typhimurium and b. stearothermophilus contain 726-731 amino acid residues per subunit. the heme-containing peroxidases of plants, fungi and yeast are monomeric, homologous and 290-350 residues in size. analyses of the amino acid sequences indicate that the double length of the bacterial peroxidases can be ascribed to gene d ... | 1991 | 1954228 |
| effect of simultaneous application of heat and pressure on the survival of bacterial spores. | the effect of simultaneous application of moderate hydrostatic pressure (10-300 atm) and heat on the survival of the bacillus stearothermophilus spores in a flow-through system was investigated. a high heterogeneity of the sensitization of spores to heat by pressure was found. a higher degree of reduction of heat resistance was observed at the low than at the high temperatures tested. the simultaneous application of moderate pressure and heat can not be applied for the preservation of liquid foo ... | 1991 | 1955421 |
| catalytic activity of aminoacyl trna synthetases and its implications for the origin of life. i. aminoacyl adenylate formation in tyrosyl trna synthetase. | the changes in the catalytic activity resulting from amino acid substitutions in the active site region have been theoretically modeled for tyrosyl trna synthetase (tyr-rs). the catalytic activity was calculated as the differential stabilization of the transition state using electrostatic approximation. the results indicate that charged residues his45, his48, asp78, asp176, asp194, lys225, lys230, lys233, arg265, and lys268 play essential roles in catalysis of aminoacyl adenylate formation in ty ... | 1991 | 1960737 |
| single-stranded dna plasmid, vector construction and cloning of bacillus stearothermophilus alpha-amylase in lactobacillus. | vector plasmids were constructed by ligating chloramphenicol and erythromycin resistance genes to taqi-digested dna of a cryptic plasmid from lactobacillus plantarum. the minimal region of lactobacillus plasmid dna that was required for dna replication was defined and a single-stranded dna intermediate replication system was observed. homologies with other origins of replication of plasmids from gram-positive bacteria, replicating via rolling circle mechanism, were found. it was shown that the c ... | 1991 | 1961976 |
| cloning, sequencing, and overexpression of genes for ribosomal proteins from bacillus stearothermophilus. | although a low resolution model for the arrangement of the proteins of the small and large ribosomal subunits is known, a detailed mechanistic understanding of the function of the ribosome awaits a high resolution structure of its components. while crystals have been obtained of several ribosomal proteins from bacillus stearothermophilus, determination of atomic resolution structures of these proteins is impeded by the difficulty of obtaining large amounts of native proteins for crystallographic ... | 1991 | 1985969 |
| detection and characterization of intermediates in the folding of large proteins by the use of genetically inserted tryptophan probes. | l-lactate dehydrogenase from bacillus stearothermophilus was rebuilt by using site-directed mutagenesis to produce an enzymically active, tryptophan-less enzyme by replacing all the wild-type tryptophans (80, 150, and 203) by tyrosines. nine single tryptophan-containing active enzymes were constructed from this enzyme by genetically replacing one of the tyrosines 36, 85, 147, 190, 203, 237, 248, 279, or 285 by tryptophan. the equilibrium and the time-resolved tryptophan fluorescence intensity an ... | 1991 | 1989674 |
| the identification of a structurally important cysteine residue in the glycerol dehydrogenase from bacillus stearothermophilus. | evidence is presented to demonstrate that the zn2+ metallo-enzyme glycerol dehydrogenase from the thermophile bacillus stearothermophilus has one cysteine residue per subunit which is only available for reaction with thiol reagents in the metal-depleted form of the enzyme. modification of the metal-depleted enzyme by methyl methanethiosulphonate prevents the reactivation of the enzyme by zn2+ ions and induces dissociation of the oligomer into subunits. the rate of reaction of the cysteine residu ... | 1991 | 2009285 |
| fluidity of bacterial membrane lipids monitored by intramolecular excimerization of 1.3-di(2-pyrenyl)propane. | intramolecular excimer formation of 1,3-di(2-pyrenyl)propane was used to study the fluidity of liposomes prepared from membrane polar lipids of bacillus stearothermophilus. on the basis of spectral data, local polarity and polarizability parameters were established suggesting that the probe molecules are located well inside the membranes, but displaced towards the polar head groups of the phospholipid molecules. the excimerization rate is very sensitive to lipid phase transitions and pretransiti ... | 1991 | 2018528 |
| promoter sequence analysis in bacillus and escherichia: construction of strong promoters in e. coli. | many derivatives of the nprm promoter of bacillus stearothermophilus and the strong early promoter, a3, of coliphage t3 were designed and chemically synthesized. these promoters consisted of some or all of the at box, consensus sequence, tac promoter sequence, spacer, and lac operator. the promoter activities were assessed by their ability to express the cat gene in escherichia coli. one of the derivatives of the a3 promoter, which contained the lac operator, was much stronger (about 3.5 times) ... | 1991 | 2022318 |
| improving the thermostability of the neutral protease of bacillus stearothermophilus by replacing a buried asparagine by leucine. | amino acids buried in the hydrophobic interior of a protein with polar side chain atoms, which are not involved in hydrogen bonding or electrostatic interactions, have an adverse effect on protein stability. replacing such residues by hydrophobic ones may render a protein more stable. asparagine 241, which is buried in the neutral protease of bacillus stearothermophilus, was replaced by leucine by site-directed mutagenesis. this mutation increased the stability of the protein by 0.7 +/- 0.1 degr ... | 1991 | 2026247 |
| correlations between kinetic and x-ray analyses of engineered enzymes: crystal structures of mutants cys----gly-35 and tyr----phe-34 of tyrosyl-trna synthetase. | the crystal structures of two mutant tyrosyl-trna synthetases (tyrts) are reported to test predictions from kinetic data about structural perturbations and also to aid in the interpretation of apparent strengths of hydrogen bonds measured by protein engineering. the enzyme-tyrosine and enzyme-tyrosyl adenylate complexes of the mutant, tyrts(cys----gly-35), have been determined at 2.5- and 2.7-a resolution, respectively. residue cys-35 is in the ribose binding site. small rearrangements in struct ... | 1991 | 2036381 |
| the cytosolic and glycosomal glyceraldehyde-3-phosphate dehydrogenase from trypanosoma brucei. kinetic properties and comparison with homologous enzymes. | the protozoan haemoflagellate trypanosoma brucei has two nad-dependent glyceraldehyde-3-phosphate dehydrogenase isoenzymes, each with a different localization within the cell. one isoenzyme is found in the cytosol, as in other eukaryotes, while the other is found in the glycosome, a microbody-like organelle that fulfils an essential role in glycolysis. the kinetic properties of the purified glycosomal and cytosolic isoenzymes were compared with homologous enzymes from other organisms. both trypa ... | 1991 | 2040304 |
| bsiy i, a novel thermophilic restriction endonuclease that recognizes 5' ccnnnnnnngg 3' and the discovery of a wrongly sequenced site in pacyc177. | a new type ii restriction endonuclease designated bsiy i has been purified from a thermophilic soil bacillus stearothermophilus strain. this enzyme recognizes and cleaves the highly degenerate sequence 5' ccnnnnn!nngg 3'. during the identification of the recognition sequence of bsiy i, we discovered that there should be five g nucleotides instead of four at position 1227-1230 of the plasmid pacyc177. | 1991 | 2041772 |
| in vivo transcriptional pattern in the infc operon of bacillus stearothermophilus. | by northern blot and primer extension analyses it was shown that in bacillus stearothermophilus the genes infc, rpmi and rplt constitute a single transcriptional unit; the promoter and the transcriptional start-point used in vivo were identified and the half-life of the transcript (1.2 min) was determined. no indication of multiple initiation sites nor of differential stability of different regions of the transcript was found. the results suggest that escherichia coli and b. stearothermophilus h ... | 1991 | 2046659 |
| cloning and analysis of the nuclear gene for yml33, a protein of the large subunit of the mitochondrial ribosome in saccharomyces cerevisiae. | the n-terminal amino acid sequence of a large subunit protein, termed yml33, of the mitochondrial ribosome of the yeast saccharomyces cerevisiae was determined. the data were obtained to synthesize two kinds of oligonucleotide primers, which were used in the polymerase chain reaction to amplify and clone the nuclear gene for this protein. by nucleotide sequencing, the cloned gene, mrp-l33, was found to encode a basic protein of 11 kda with 98 amino acid residues. the protein encoded by this gene ... | 1991 | 2061283 |
| [molecular cloning and structural-functional analysis of the arginine biosynthesis genes of the thermophilic bacterium bacillus stearothermophilus]. | genes encoding arginine biosynthesis of bacillus stearothermophilus strain 718 were cloned in the mutant arga strain of the escherichia coli k-12. the arg genes were shown to be located on the 3.7 kb dna fragment in the following order: arga--arge--argb. the expression of the arga gene of b. stearothermophilus on the multicopy vehicle is twofold higher in argr- strain of e. coli k-12 than is isogenic argr+ strain. according to hybridization analysis arga genes of b. stearothermophilus and b. sub ... | 1990 | 2074006 |
| enhancement of thermostability of neutral proteases. | | 1990 | 2075976 |
| thermostable glucokinase from bacillus stearothermophilus and its analytical application. | | 1990 | 2075989 |
| atp binding plays a role in the selection of amino acid substrate by aminoacyl-trna synthetases. | | 1990 | 2075999 |
| nucleotide sequence and cloning in bacillus subtilis of the bacillus stearothermophilus pleiotropic regulatory gene degt. | the regulatory gene (degt) from bacillus stearothermophilus nca1503 which enhanced production of extracellular alkaline protease (apr) was cloned in bacillus subtilis with ptb53 as a vector. when b. subtilis mt-2 (npr- [deficiency of neutral protease] apr+) was transformed with the recombinant plasmid, pdt145, the plasmid carrier produced about three times more alkaline protease than did the wild-type strain. in contrast, when b. subtilis db104 (npr- apr-) was used as a host, the transformant wi ... | 1990 | 2104607 |
| vapor-phase hydrogen peroxide as a surface decontaminant and sterilant. | the feasibility of utilizing vapor-phase hydrogen peroxide (vphp) as a surface decontaminant and sterilant was evaluated in a centrifuge application. the prototype vphp decontamination system, retrofitted into a beckman l8-m ultracentrifuge, was designed to vaporize a 30% (wt/wt) solution of aqueous hydrogen peroxide continuously injecting and withdrawing vphp in a deep-vacuum flow-through system. vphp cycles of 4, 8, 16, and 32 min were examined for cidal activity against spores of bacillus sub ... | 1990 | 2106287 |
| construction and properties of a temperature-sensitive mutation in the gene for the bacteriophage spo1 dna-binding protein tf1. | the bacillus subtilis bacteriophage spo1 encodes the dna-binding protein tf1, a homolog of the ubiquitous type ii dna-binding proteins that are components of bacterial chromatin. the known three-dimensional structure of a related protein was used in devising a scheme of site-directed mutagenesis that led to the creation of a temperature-sensitive mutation in the tf1 gene. at the nonpermissive temperature, this mutation disrupted the temporal regulation of viral protein synthesis and processing, ... | 1990 | 2115873 |
| characterization of a thermostable bacillus stearothermophilus alpha-amylase. | liquefying-type bacillus stearothermophilus alpha-amylase was characterized. the coding gene was cloned in bacillus subtilis and the enzyme was produced in three different host organisms: b. stearothermophilus, b. subtilis, and escherichia coli. properties of the purified enzyme were similar irrespective of the host. temperature optimum was at 70-80 degrees c and ph optimum at 5.0-6.0. the enzyme was stable for 1 h in the ph range 6.0-7.5 at 80 degrees c. the enzyme was stabilized by ca2+, na+, ... | 1990 | 2119192 |
| glutamyl-trna synthetases of bacillus subtilis 168t and of bacillus stearothermophilus. cloning and sequencing of the gltx genes and comparison with other aminoacyl-trna synthetases. | the glutamyl-trna synthetase (glurs) of bacillus subtilis 168t aminoacylates with glutamate its homologous trna(glu) and trna(gln) in vivo and escherichia coli trna(1gln) in vitro (lapointe, j., duplain, l., and proulx, m. (1986) j. bacteriol. 165, 88-93). the gltx gene encoding this enzyme was cloned and sequenced. it encodes a protein of 483 amino acids with a mr of 55,671. alignment of the amino acid sequences of four bacterial glurss (from b. subtilis, bacillus stearothermophilus, e. coli, a ... | 1990 | 2120226 |
| thermal analysis of bacteria by differential scanning calorimetry: relationship of protein denaturation in situ to maximum growth temperature. | differential scanning calorimetry (dsc) was used to analyze thermal transitions in two strains of the thermophile bacillus stearothermophilus (atcc 12016 and wat), the mesophile bacillus megaterium and the psychrotroph bacillus psychrophilus. the observed transitions, representing lipid melting and dna and protein unfolding, are compared to the maximum growth temperature (tmax) in each species as a means of identifying critical, thermolabile targets responsible for heat-induced inhibition of gro ... | 1990 | 2121283 |
| growth of bacillus stearothermophilus on glycerol in chemostat culture: expression of an unusual phenotype. | bacillus stearothermophilus grew readily on glycerol in carbon-limited chemostat culture and expressed a high carbon conversion efficiency. however, the strain of organism used (probably b. stearothermophilus var. nondiastaticus) proved particularly sensitive to glycerol, both respiration and growth being severely impeded by any surfeit of this compound. sensitivity was found to correlate with an exceptionally high level of expression of glycerol kinase [activities of more than 80 mumol min-1 (m ... | 1990 | 2121901 |
| purification and properties of thermostable xylanase and beta-xylosidase produced by a newly isolated bacillus stearothermophilus strain. | we isolated a thermophilic bacterium that produces both xylanase and beta-xylosidase. based on taxonomical research, this bacterium was identified as bacillus stearothermophilus. each extracellular enzyme was separated by hydrophobic chromatography by using a toyopearl hw-65 column, followed by gel filtration with a sephacryl s-200 column. each enzyme in the culture was further purified to homogeneity (62-fold for xylanase and 72-fold for beta-xylosidase) by using a fast protein liquid chromatog ... | 1990 | 2123854 |
| the nicotinamide subsite of glyceraldehyde-3-phosphate dehydrogenase studied by site-directed mutagenesis. | directed mutagenesis has been used to study the nicotinamide subsite of the glycolytic nad-dependent glyceraldehyde-3-phosphate dehydrogenase (gapdh). residue asn313 is involved together with the carboxyamide moiety of the nicotinamide ring in a complex network of hydrogen bonding interactions which fix the position of the pyridinium ring of nad to which hydride transfer occurs at the c-4 position in the catalytic reaction. the asparagine side-chain has been replaced by that of the thr and ala r ... | 1990 | 2126460 |
| recognition of trna(tyr) by tyrosyl-trna synthetase. | in this review, i have brought together and compared the available data on the interaction between trna(tyr) and tyrosyl-trna synthetases (tyrts) of prokaryotic origins. the amino acid sequences of the heterologous tyrts that can charge escherichia coli trna(tyr), show that the residues involved in the binding and recognition of tyrosine are strictly conserved whereas those involved in the interaction with trna(tyr) are only weakly similar. the results of in vivo genetic complementation experime ... | 1990 | 2126463 |
| heat sterilization of bioindicators in propylene glycol and propylene glycol-water mixtures: arrhenius equation, thermodynamic data, and z values. | our interest in calculating the thermodynamic data by means of the arrhenius equation was based on two observation: (a) the thermal death time increases considerably when the bioindicators bacillus subtilis var. niger and bacillus stearothermophilus are sterilized in nonaqueous hydrophilic solutions as found in propylene glycol (pg) with low water concentrations; and (b) the inactivation kinetics of bac. stearothermophilus does not follow a first-order reaction. the frequency factor a and the en ... | 1990 | 2128895 |
| evaluation of sterilization monitoring for dental offices in ohio. | | 1990 | 2129135 |
| dynamic ultraviolet sterilization of different implant types. | this paper investigates the use of the dynamic ultraviolet sterilization process with various dental implants, stainless steel orthopedic cortical bone screws, and polysulfone polymer healing caps. these biomaterials were inoculated with the spores of bacillus subtilis and bacillus stearothermophilus. they were then exposed to dynamic ultraviolet radiation in the chamber of a bud ultraviolet device. samples were incubated in trypticase soy broth at 37 degrees c and 56 degrees c, and they were su ... | 1990 | 2133336 |
| presence of the bacterial hemoglobin gene improves alpha-amylase production of a recombinant escherichia coli strain. | a recombinant plasmid (pmk57) was constructed by cloning the bacillus stearothermophilus alpha-amylase gene into puc8; plasmid pmk79 was then derived from pmk57 by inserting the bacterial (vitreoscilla) hemoglobin gene into the latter plasmid. both pmk57 and pmk79 were transformed into escherichia coli strain jm 103 to make strains mk57 and mk79, respectively. both mk57 and mk79 produced alpha-amylase and mk79 produced hemoglobin. mk79 outgrew mk57 in shake flasks in lb medium, the advantage of ... | 1990 | 2136531 |
| structural basis of the allosteric behaviour of phosphofructokinase. | comparison between the crystal structures of low- and high-affinity forms of phosphofructokinase shows a close coupling between the change of quaternary structure and local changes triggered by binding of the allosteric effectors. these concerted changes link all the substrate and effector sites in the tetramer, and explain the change of affinity for the cooperative substrate. | 1990 | 2136935 |
| the rabbit muscle phosphofructokinase gene: cdna cloning and sequencing. | a partial cdna for rabbit muscle phosphofructokinase (rm-pfk) has been cloned and sequenced. the nucleotide sequence of the cdna agrees with the previously determined rm-pfk genomic sequence. in addition, the amino acid sequence deduced from the cdna is nearly identical to the rm-pfk sequence previously determined by peptide analysis. a significant degree of homology exists when the amino acid sequence of rm-pfk is compared with the sequences of bacillus stearothermophilus pfk or escherichia col ... | 1990 | 2147292 |
| stereochemistry and lifetime of the gtp hydrolysis intermediate at the active site of elongation factor tu from bacillus stearothermophilus as inferred from the 17o-55mn superhyperfine interaction. | electron paramagnetic resonance spectroscopy has been used to obtain information on the structure and stability of the products of gtp cleavage at the active site of elongation factor tu (ef-tu) from bacillus stearothermophilus. using stereospecifically labelled (sp)-(rp)-[beta-17o]gtp (prepared by modification of a previously published procedure which is now also suitable for guanine nucleotides), it was found that only one of the two possible diastereomers (sp) led to detectable line-broadenin ... | 1990 | 2156700 |
| purification and characterization of thermostable beta-mannanase and alpha-galactosidase from bacillus stearothermophilus. | bacillus stearothermophilus secretes beta-mannanase and alpha-galactosidase enzymatic activities capable of hydrolyzing galactomannan substrates. expression of the hemicellulase activities in the presence of locust bean gum was sequential, with mannanase activity preceding expression of alpha-galactosidase activity. the hemicellulase activities were purified to homogeneity by a combination of ammonium sulfate fractionation, gel filtration, hydrophobic interaction chromatography, and ion-exchange ... | 1990 | 2176449 |
| does single-amino-acid replacement work in favor of or against improvement of the thermostability of immobilized enzyme? | thermostabilities of kanamycin nucleotidyltransferase and of its mutants that became thermostable, in the free state, because of single-amino-acid replacements were studied after immobilization of the enzymes on cyanogen bromide-activated sephadex g-200 particles. lys in place of gln at position 102 decreased the thermostability of the immobilized enzyme, whereas replacement with other amino acids enhanced it. | 1990 | 2176451 |
| evidence that chemical modification of a positively charged residue at position 189 causes the loss of catalytic activity of iron-containing and manganese-containing superoxide dismutases. | the escherichia coli, bacillus stearothermophilus, and human manganese-containing superoxide dismutases (mnsods) and the e. coli iron-containing superoxide dismutase (fesod) are extensively inactivated by treatment with phenylglyoxal, an arginine-specific reagent. arg-189, the only conserved arginine in the primary sequences of these four enzymes, is also conserved in the three additional fesods and five of the six additional mnsods sequenced to date. the only exception is saccharomyces cerevisi ... | 1990 | 2186704 |
| thermostable alanine racemase. its structural stability. | the gene encoding thermostable alanine recemase from bacillus stearothermophilus was cloned and expressed in e. coli. the enzyme was purified to homogeneity from cell extracts of e. coli carrying a plasmid designated picr4. the alanine racemase gene sequenced was found to contain an open reading frame of 1158 nucleotides. the molecular weight of the enzyme subunit was estimated to be 43,341. the alpha-helical and beta-structure contents were calculated to be about 34 and 26%, respectively, from ... | 1990 | 2192620 |
| expression in escherichia coli of a sub-gene encoding the lipoyl domain of the pyruvate dehydrogenase complex of bacillus stearothermophilus. | a sub-gene encoding the lipoyl domain (residues 1-85) of the lipoate acetyltransferase chain of the pyruvate dehydrogenase complex of bacillus stearothermophilus was over-expressed in escherichia coli. approx. 80% of the domain was unlipoylated but most of the remainder was correctly lipoylated on lys-42 and could be reductively acetylated by the b stearothermophilus enzyme complex. a small proportion (approx. 4%) of the domain carried an aberrant substituent, possibly an octanoyl group, on lys- ... | 1990 | 2192914 |
| amino acid sequences of the ribosomal proteins hl30 and hmal5 from the archaebacterium halobacterium marismortui. | the complete amino acid sequences of the ribosomal proteins hl30 and hmal5 from the archaebacterium halobacterium marismortui were determined. protein hl30 was found to be acetylated at its n-terminal amino acid and shows homology to the eukaryotic ribosomal proteins yl34 from yeast and rl31 from rat. protein hmal5 was homologous to the protein l5 from escherichia coli and bacillus stearothermophilus as well as to yl16 from yeast. hmal5 shows more similarities to its eukaryotic counterpart than ... | 1990 | 2198942 |
| cloning and sequence analysis of the genes encoding the alpha and beta subunits of the e1 component of the pyruvate dehydrogenase multienzyme complex of bacillus stearothermophilus. | a 4175-bp ecori fragment of dna that encodes the alpha and beta chains of the pyruvate dehydrogenase (lipoamide) component (e1) of the pyruvate dehydrogenase multienzyme complex of bacillus stearothermophilus has been cloned in escherichia coli. its nucleotide sequence was determined. open reading frames (pdha, pdhb) corresponding to the e1 alpha subunit (368 amino acids, mr 41,312, without the initiating methionine residue) and e1 beta subunit (324 amino acids, mr 35,306, without the initiating ... | 1990 | 2200674 |
| cloning and nucleotide sequences of the bacillus stearothermophilus neutral protease gene and its transcriptional activator gene. | both the neutral protease gene (nprs) and its transcriptional activator gene (npra) from bacillus stearothermophilus telne were cloned in bacillus subtilis by using ptb53 as a vector plasmid. the presence of the npra gene enhanced protease synthesis by about fivefold. the nucleotide sequences of nprs and its flanking regions were determined. nprs was composed of 1,653 base pairs and 551 amino acid residues. a shine-dalgarno (sd) sequence was found 9 bases upstream from the translation start site ... | 1990 | 2203733 |
| expression of the copy dna for human a4 and b4 l-lactate dehydrogenases in escherichia coli. | the human ldh-a and ldh-b cdnas, containing the coding regions for the l-lactate dehydrogenase a4 (m) and b4 (h) polypeptides respectively have been cloned into escherichia coli to place the cdnas under the control of hybrid e. coli/bacillus stearothermophilus transcriptional and translational signals. human a4- and b4-isoenzymes are produced in e. coli cells harbouring the expression plasmids phldha22 and phldhb10 at levels of 6.5 and 1.5% of the soluble protein of the cell, respectively. the t ... | 1990 | 2205297 |
| structure and function of l-lactate dehydrogenases from thermophilic and mesophilic bacteria, x. analysis of structural elements responsible for the differences in thermostability and activation by fructose 1,6-bisphosphate in the lactate dehydrogenases from b. stearothermophilus and b. caldolyticus by protein engineering. | the amino-acid sequences of the lactate dehydrogenases (ldh) from b. stearothermophilus and b. caldolyticus differ at only 10 positions. the properties of these enzymes however show substantial differences. the ldh from b. stearothermophilus is activated by fru-p2 and has a higher thermostability (10 degrees c) than the enzyme from b. caldolyticus which cannot be activated by fru-p2. to correlate these functional differences to the structural properties, we have constructed a set of hybrid- and ... | 1990 | 2206453 |
| nucleotide sequences of bacillus stearothermophilus ribosomal protein genes: part of the ribosomal s10 operon. | restriction fragments from bacillus stearothermophilus chromosomal dna were cross-hybridized with the escherichia coli ribosomal protein l2 gene rplb. a 2-kb ecori fragment which showed cross-hybridization was cloned into the m13 phage and sequenced by the dideoxy chain-terminating method. comparison of the deduced amino-acid sequences with the corresponding sequences of e. coli ribosomal proteins showed that this fragment contains the region encoding the c-terminus of l2, the genes encoding s19 ... | 1990 | 2222862 |
| probing the coenzyme specificity of glyceraldehyde-3-phosphate dehydrogenases by site-directed mutagenesis. | by combining our knowledge of the crystal structure of the glycolytic nad-dependent glyceraldehyde-3-phosphate dehydrogenase (gapdh) and the sequence of the photosynthetic nadp-dependent gapdh of the chloroplast, two particular amino acid residues were predicted as the principal determinants of differing coenzyme specificity. by use of site-directed mutagenesis, the amino acids leu 187 and pro 188 of gapdh from bacillus stearothermophilus have been replaced with ala 187 and ser 188, which occur ... | 1990 | 2223764 |
| a comparative study of ribosomal and dna binding protein ii from two thermophilic bacteria, bacillus caldolyticus strain ep 00275 and bacillus stearothermophilus. | ribosomal and dna binding proteins (dna bp ii) from an extreme thermophilic bacterium, b. caldolyticus strain ep 00275, were investigated for stability and crystallization and compared to the homologous proteins from b. stearothermophilus. two-dimensional gel electrophoresis of both types of proteins, the amino acid composition and the sequences of some of the peptides of dna bp ii revealed a close relationship between each other. the physico-chemical characteristics of dna bp ii were similar bu ... | 1990 | 2226856 |
| use of a hapten specific anti-dansyl antibody for the localization of ribosomal proteins by immuno electron microscopy. | the fluorescent reagent dansyl chloride has been used as an immunological marker for the electron microscopic localization of ribosomal proteins on the surface of 50s ribosomal subunits. the proteins bstl1 from bacillus stearothermophilus and ecol1 from escherichia coli were dansylated to various degrees and reconstituted into the l1-deficient e. coli 50s subunits from mutant mv17-10. using antibodies specific to dansyl chloride, both proteins were mapped at the lateral protuberance near the pep ... | 1990 | 2242000 |
| [construction of promoter probe vector pfdc4 and gene expression vector pfdc11 with high transformation efficiency in bacillus stearothermophilus]. | a 0.5kb fragment from sau3a-digested total dna of bacillus stearothermophilus cu21 was cloned with the vector ppgv5, a derivative of ppl703. the insertion of this fragment can activate the expression of the promoteless cat-86 gene on the cloning vector in both b. stearothermophilus and bacillus subtilis hosts. when the 0.54 kb fragment is present in ppgv5 in either orientation, the transformation efficiency of the plasmid is increased about 10(3) to 10(4) fold in cu21 protoplasts. southern hybri ... | 1990 | 2242283 |
| [a novel type of phase variation regarding integrated and free states of plasmid pfdx163 in bacillus stearothermophilus cu21]. | pfdx1 is a recombinant plasmid which carries a foreign gene xyle. by selecting for kanamycin-resistant mutants of bacillus stearothermophilus cu21(pfdx1) at higher temperature, a variant strain cu21-163 was obtained. this strain harbors a mutant plasmid pfdx163, which was formed by insertion of a 2.0kb h-fragment from the cu21 genome onto the plasmid pfdx1. pfdx163 was supposed to be integrated into the cu21 chromosome via homologous recombination of h-fragments. the cu21-163 strain consists of ... | 1990 | 2252599 |
| cloning and sequence analysis of the genes encoding the dihydrolipoamide acetyltransferase and dihydrolipoamide dehydrogenase components of the pyruvate dehydrogenase multienzyme complex of bacillus stearothermophilus. | a 2641-bp ecori fragment of dna that encodes the c-terminal part of the dihydrolipoyl acetyltransferase (e2) component and the dihydrolipoamide dehydrogenase (e3) component of the pyruvate dehydrogenase complex of bacillus stearothermophilus has been cloned in escherichia coli. its nucleotide sequence was determined. a 705-bp truncated open reading frame was located at the 5'end of the insert which, together with the 588-bp truncated open reading frame at the 3' end of another ecori fragment of ... | 1990 | 2253629 |
| isolation and characterization of bacillus stearothermophilus 30s and 50s ribosomal protein mutations. | bacillus stearothermophilus mutations which confer resistance to or dependence on a variety of ribosome-targeted antibiotics have been isolated. many of these mutations produce ribosomal proteins with altered mobilities in a two-dimensional gel electrophoresis system. this collection of altered thermophilic ribosomal proteins will be useful in examining ribosomal structure and function. | 1990 | 2254291 |
| proteolysis of bacillus stearothermophilus if2 and specific protection by gtp. | translation initiation factor if2 from bacillus stearothermophilus (741 amino acids, mr = 82,043) was subjected to trypsinolysis alone or in the presence of gtp. following electroblotting and automated amino acid sequencing of the resulting peptides, the location and the sequential order of the main cleavage sites were identified. trypsinolysis of if2 ultimately generates two compact domains: a 24.5 kda c-terminal fragment and a 40 kda g-fragment which is obtained only in the presence of gtp whi ... | 1990 | 2265694 |
| designs for a broad substrate specificity keto acid dehydrogenase. | variations have been made to the structure of the nicotinamide adenine dinucleotide (nad) dependent l-lactate dehydrogenase from bacillus stearothermophilus at regions of the enzyme that we believe determine specificity toward different alpha-hydroxy acids (rchohcoo-, r = ch3, c2h5, etc.). two regions of ldh that border the active site (but are not involved in the catalytic reaction) were altered in order to accommodate substrates with hydrophobic side chains larger than that of the naturally pr ... | 1990 | 2271542 |
| identification of a reversible structural transition in the metal-depleted glycerol dehydrogenase from bacillus stearothermophilus. | evidence is presented to demonstrate that the zn2+ -depleted, inactive form of the glycerol dehydrogenase from bacillus stearothermophilus exists in one of two possible conformations in equilibrium, the position of which is temperature sensitive. the conformation of the metal-depleted enzyme favoured by higher temperatures (20-40 degrees c) is able to bind zn2+ and regain catalytic activity, whereas that favoured at lower temperatures (0-10 degrees c) is unable to bind metal ions and is thus ina ... | 1990 | 2294019 |
| genetic map of the bacillus stearothermophilus nub36 chromosome. | a circular genetic map of bacillus stearothermophilus nub36 was constructed by transduction with bacteriophage tp-42c and protoplast fusion. sixty-four genes were tentatively assigned a cognate bacillus subtilis gene based on growth response to intermediates or end products of metabolism, cross-feeding, accumulation of intermediates, or their relative order in a linkage group. although the relative position of many genes on the bacillus stearothermophilus and bacillus subtilis genetic map appear ... | 1990 | 2298700 |
| conserved residues of liquefying alpha-amylases are concentrated in the vicinity of active site. | three dimensional structure of three liquefying type bacillus alpha-amylases were modeled based on sequence analyses and refined structure of aspergillus oryzae enzyme. the models suggest that the overall folding motif of alpha-amylases is conserved. the active site, substrate binding and stabilizing calcium binding residues are conserved and concentrated in a cleft between two domains. they constitute the core of alpha-amylases to which other, less conserved regions are attached. the bacterial ... | 1990 | 2302216 |
| a single amino acid substitution in lactate dehydrogenase improves the catalytic efficiency with an alternative coenzyme. | using site-directed mutagenesis, the nadh-linked lactate dehydrogenase from bacillus stearothermophilus has been specifically altered at a single residue to shift the coenzyme specificity towards nadph. the single change is at position 53 in the amino acid sequence where a conserved aspartate has been replaced by a serine. this substitution was made to reduce steric hindrance on binding of the extra phosphate group of nadph and to remove the negative charge of the aspartate group. the resultant ... | 1990 | 2302233 |
| a new type ii restriction endonuclease, bsma i, from bacillus stearothermophilus. | | 1990 | 2308865 |
| colorimetric glucose assay using thermostable glucokinase. | a method for assaying glucose in serum or plasma samples using a thermostable glucokinase was developed. glucokinase from bacillus stearothermophilus was coupled with glucose-6-phosphate dehydrogenase to produce nadph, which reduced the tetrazolium dye mtt to its formazan. detection of the product at 660 nm allowed samples containing up to 30 mmol/l glucose to be assayed with an endpoint method. use of the optimal wavelength for formazan detection, 570 nm, increased sensitivity for nadph detecti ... | 1990 | 2310155 |
| direct phase determination for the molecular envelope of tryptophanyl-trna synthetase from bacillus stearothermophilus by x-ray contrast variation. | monoclinic crystals of bacillus stearothermophilus tryptophanyl-trna synthetase grown in the presence of substrate tryptophan (space group p2(1)) display evidence of a low-resolution trigonal space group (p321). the origin and averaging transformations for the local 32 point group of this unusually clear sixfold non-crystallographic symmetry may be inferred without prior estimation of the electron density. this local symmetry was exploited in conjunction with solvent density contrast variation t ... | 1990 | 2310535 |
| simultaneous comparison of several sequences. | | 1990 | 2314287 |
| electron transferase activity of diaphorase (nadh: acceptor oxidoreductase) from bacillus stearothermophilus. | electrochemical kinetic measurements were carried out for electron-transfer between nadh and the oxidized forms of mediators (ferrocenylmethanol (fma), ferrocenyl-1-ethanol (fea), n,n,n',n'-tetramethylphenylenediamine (tmpd), co(phen)2+(3) and fe(cn)4-(6)) catalyzed by diaphorase (nadh: acceptor oxidoreductase, ec 1.6.99.-) purified from bacillus stearothermophilus. cyclic voltammograms for the mediators with excess nadh in the presence of diaphorase gave steady-state currents. the quantitative ... | 1990 | 2317516 |
| isolation and identification of restriction endonuclease bsiki. | | 1990 | 2326209 |
| random mutagenesis used to probe the structure and function of bacillus stearothermophilus alpha-amylase. | mutations that cover the sequence of bacillus stearothermophilus alpha-amylase were produced by an efficient in vitro enzymatic random mutagenesis method and the mutant alpha-amylases were expressed in escherichia coli, which also secreted the product. ninety-eight mutants were identified by sequencing and their enzyme activities were classified into three classes: wild-type, reduced or null. a molecular model of the enzyme was constructed using the coordinates of takaamylase a and a consensus a ... | 1990 | 2330367 |
| structure determination and refinement of bacillus stearothermophilus lactate dehydrogenase. | structures have been determined of bacillus stearothermophilus "apo" and holo lactate dehydrogenase. the holo-enzyme had been co-crystallized with the activator fructose 1,6-bisphosphate. the "apo" lactate dehydrogenase structure was solved by use of the known apo-m4 dogfish lactate dehydrogenase molecule as a starting model. phases were refined and extended from 4 a to 3 a resolution by means of the noncrystallographic molecular 222 symmetry. the r-factor was reduced to 28.7%, using 2.8 a resol ... | 1990 | 2330370 |
| metal ion dependence of phosphorothioate atp analogues in the bacillus stearothermophilus tyrosyl-trna synthetase reaction. | pre-steady-state kinetic analyses on the formation of tyrosyl adenylate from tyrosine and each of the four diastereomers of alpha- and beta-phosphorothioate adenosine triphosphates [atp alpha s and atp beta s; eckstein, f., & goody, r. (1976) biochemistry 15, 1685-1691; yee, d., armstrong, v. w., & eckstein, f. (1979) biochemistry 18, 4116-4123] were performed in the presence of mg2+, co2+, and cd2+ as the divalent metal ion cofactor. a modest preference of 5.5-fold in kappa 3/ka' (where kappa 3 ... | 1990 | 2334722 |
| identification of a new type ii restriction endonuclease, bsaai. | | 1990 | 2339077 |
| alanine dehydrogenases from two bacillus species with distinct thermostabilities: molecular cloning, dna and protein sequence determination, and structural comparison with other nad(p)(+)-dependent dehydrogenases. | the gene encoding alanine dehydrogenase (ec 1.4.1.1) from a mesophile, bacillus sphaericus, was cloned, and its complete dna sequence was determined. in addition, the same gene from a moderate thermophile, b. stearothermophilus, was analyzed in a similar manner. large parts of the two translated amino acid sequences were confirmed by automated edman degradation of tryptic peptide fragments. each alanine dehydrogenase gene consists of a 1116-bp open reading frame and encodes 372 amino acid residu ... | 1990 | 2340274 |
| composition of polar lipid acyl chains of bacillus stearothermophilus as affected by temperature and calcium. | bacillus stearothermophilus was grown within the temperature range of 48 to 68 degrees c in a complex medium and in the range of 45 to 72 degrees c in the presence of 2.5 mm ca2+. the main fatty acids of lipid extracts contain 15 to 17 carbon atoms, mostly branched-chain species. the most prevalent saturated straight-chain fatty acid is n-c16. the total amount of branched-chain species decreases with increasing temperature of growth from 48 to 68 degrees c, whereas the straight-chain species inc ... | 1990 | 2369582 |
| [expression of xyle gene in bacillus stearothermophilus]. | using the gene expression vector pfdc11 for bacillus stearothermophilus cu21, a recombinant plasmid pfdx1 was constructed, which carries the pseudomonas-derived xyle gene coding for catechol 2,3-dioxygenase (cato2ase). cato2ase activity can be detected from cu21 (pfdx1) cells grown at 48 degrees c. this result indicates that the promoterless xyle gene can be expressed in a thermophilic host under the direction of a promoter from a thermophilic bacterium. by selecting kmr mutants at elevated grow ... | 1990 | 2372405 |
| studies on the interactions of glycerol dehydrogenase from bacillus stearothermophilus with zn2+ ions and nadh. | the interactions of the essential divalent cation, zn2+, with the binary complex formed between glycerol dehydrogenase (glycerol:nad+ 2-oxidoreductase, ec 1.1.1.6) and its coenzyme nadh have been examined by fluorescence spectroscopy. both the metallo and non-metallo form of the enzyme bind the coenzyme nadh. the addition of zn2+ ions to a solution of the binary complex formed between metal-depleted enzyme and nadh results in a rapid increase in fluorescence emission at 430 nm. this has been use ... | 1990 | 2378897 |
| [defective function of bioindicators for steam sterilization]. | it can be shown, that under certain conditions commercially available indicators with bacillus stearothermophilus and packages of native spores from soil prepared according to din 58 946/4 react differently to treatment in a lab-type steam sterilizer. the differences were most evident when incomplete evacuation of air had to be supposed. these results lead to the conclusion that some bioindicators are not able to show the inefficient function of steam sterilizers caused by local residuals of air ... | 1990 | 2393488 |
| characterization of the bacillus stearothermophilus manganese superoxide dismutase gene and its ability to complement copper/zinc superoxide dismutase deficiency in saccharomyces cerevisiae. | recombinant clones containing the manganese superoxide dismutase (mnsod) gene of bacillus stearothermophilus were isolated with an oligonucleotide probe designed to match a part of the previously determined amino acid sequence. complementation analyses, performed by introducing each plasmid into a superoxide dismutase-deficient mutant of escherichia coli, allowed us to define the region of dna which encodes the mnsod structural gene and to identify a promoter region immediately upstream from the ... | 1990 | 2407726 |
| potential metal-binding domains in nucleic acid binding proteins. | a systematic search for sequences that potentially could form metal-binding domains in proteins has been performed. five classes of proteins involved in nucleic acid binding or gene regulation were found to contain such sequences. these observations suggest numerous experiments aimed at determining whether metal-binding domains are present in these proteins and, if present, what roles such domains play in the processes of nucleic acid binding and gene regulation. | 1986 | 2421409 |
| evidence for two activated forms of pyruvate kinase from bacillus stearothermophilus in the presence of ribose 5-phosphate. | allosteric activation of pyruvate kinase from a thermophilic bacterium, bacillus stearothermophilus, by ribose 5-phosphate (r5p) was kinetically examined. two activated forms of this enzyme could be distinguished, depending on the r5p concentration. one form (form i) was observed at about 10(-5) m r5p. it showed a slightly negative cooperativity for phosphoenolpyruvate (pep). the other form (form ii) was observed at more than 10(-3) m r5p and showed michaelis-menten kinetics for pep. the pep and ... | 1987 | 2439493 |
| molecular sieving through s layers of bacillus stearothermophilus strains. | the permeability properties and the exclusion limits of the crystalline surface layers (s layers) of two selected strains of bacillus stearothermophilus were investigated. measurements were performed of passive solute uptake into the intracellular space of native or glutaraldehyde-treated sacculi. native sacculi were prepared from whole cells by extracting the cytoplasmic membrane with triton x-100 under conditions which preserved the integrity of the s layer and the peptidoglycan-containing lay ... | 1987 | 2442137 |
| three-dimensional image reconstruction from ordered arrays of 70s ribosomes. | a better understanding of the molecular mechanism of protein biosynthesis still awaits a reliable model for the ribosomal particle. we describe here the application of a diffraction technique, namely three-dimensional image reconstruction from two-dimensional sheets of 70s ribosomes from bacillus stearothermophilus at 47 a resolution. the three-dimensional model obtained by these studies shows clearly the two subunits, the contact points between them, an empty space large enough to accommodate t ... | 1987 | 2450592 |
| comparative 16s rrna oligonucleotide analyses and murein types of round-spore-forming bacilli and non-spore-forming relatives. | the phylogenetic incoherency of the genus bacillus as presently described is demonstrated by analysis of both published and new data from comparative 16s rrna cataloguing of nine bacillus species and a number of related non-bacillus taxa, i.e. caryophanon latum, filibacter limicola and planococcus citreus. while the ellipsoidal-spore-forming bacilli, e.g. b. subtilis and allied species, formed a coherent cluster, the round-spore-forming bacilli showed a higher degree of relationship to the non-s ... | 1987 | 2452227 |
| in vitro incorporation of eubacterial, archaebacterial and eukaryotic 5s rrnas into large ribosomal subunits of bacillus stearothermophilus. | bacillus stearothermophilus large ribosomal subunits were reconstituted in the presence of 5s rrnas from different origins and tested for their biological activities. the results obtained have shown that eubacterial and archaebacterial 5s rrnas can easily substitute for b. stearothermophilus 5s rrna in the reconstitution, while eukaryotic 5s rrnas yield ribosomal subunits with reduced biological activities. from our results we propose an interaction between nucleotides 42-47 of 5s rrna and nucle ... | 1988 | 2453840 |
| three-dimensional location of ribosomal protein bl2 from bacillus stearothermophilus, a key component of the peptidyl transferase center. | protein bl2 from bacillus stearothermophilus has been localized by immunoelectron microscopy on the interface side of the 50 s subunit, beneath the angle formed between the central protuberance and the l1 protuberance. the immuno-electron microscopic data suggest that the interface region of the 50 s particle is not as flat as most of the proposed three-dimensional models suggest, but instead there is a significant concavity. since several studies demonstrated that bl2 is implicated in peptidyl ... | 1988 | 2454842 |
| effect of growth temperature and media composition on the fatty acid composition of bacillus stearothermophilus an 002. | the influence of growth temperature, media composition and cell age on the chemical composition of bacillus stearothermophilus strain an 002 has been determined. the total cellular protein decreased and the free amino acid content increased with growth temperature, in both exponential and stationary growth phase. the protein and free amino acid contents of cells were higher in the stationary phase than in the exponential phase, irrespective of growth temperature and media composition. the rna co ... | 1988 | 2455474 |