| codon-optimized glucoamylase sgai of aspergillus awamori improves starch utilization in an industrial yeast. | the development of a yeast that converts raw starch to ethanol in one step (called consolidated bioprocessing) could yield large cost reductions in the bioethanol industry. the aim of this study was to develop an efficient amylolytic saccharomyces cerevisiae strain suitable for industrial bioethanol production. a native and codon-optimized variant of the aspergillus awamori glucoamylase gene were expressed in the s. cerevisiae y294 laboratory strain. codon optimization resulted to be effective a ... | 2012 | 22450569 |
| garcina cambogia leaf and seawater for tannase production by marine aspergillus awamori btmfw032 under slurry state fermentation. | garcinia gummi-gutta (syn. g. cambogia, g. quaesita), known to have medicinal properties, was evaluated as a substrate and inducer for tannase production by a marine aspergillus awamori btmfw032, under slurry state fermentation using czapekdox-minimal medium and sea water as the cultivation medium. among the various natural tannin substrates evaluated, garcinia leaf supported maximal tannase production. the cultivation conditions and components of the cultivation medium were optimized employing ... | 2011 | 22312743 |
| the modification of the muscle fatty acid profile by dietary supplementation with aspergillus awamori in broiler chickens. | the present study was conducted to show that dietary supplementation with a fungus, aspergillus awamori, modifies muscle fatty acid profiles in broiler chickens. a total of thirty chicks, selected from a group of 100 chicks aged 15 d, were divided into a control group and two treatment groups (ten birds per treatment). the control group was fed a basal diet, and the treatment groups were fed basal diets supplemented with a. awamori at levels of 0·05 and 0·2 %. from the start of the study at 15 d ... | 2012 | 22289635 |
| casein phosphopeptides drastically increase the secretion of extracellular proteins in aspergillus awamori. proteomics studies reveal changes in the secretory pathway. | the secretion of heterologous animal proteins in filamentous fungi is usually limited by bottlenecks in the vesicle-mediated secretory pathway. | 2012 | 22234238 |
| xylanase production by aspergillus awamori under solid state fermentation conditions on tomato pomace. | in this work, tomato pomace, a waste abundantly available in the mediterranean and other temperate climates agro-food industries, has been used as raw material for the production of some hydrolytic enzymes, including xylanase, exo-polygalacturonase (exo-pg), cellulase (cmcase) and α-amylase. the principal step of the process is the solid state fermentation (ssf) of this residue by aspergillus awamori. in several laboratory experiments, maximum xylanase and exo-pg activities were measured during ... | 2011 | 24031793 |
| fungal isolates from natural pectic substrates for polygalacturonase and multienzyme production. | pectin rich wastes and waste dump yard soils were screened and eighty pectinolytic fungal isolates were obtained by enrichment culturing and ruthenium red plate assay. eight isolates with higher zones of pectin hydrolysis were selected and tested for polygalacturonase production. one isolate identified as aspergillus awamori mtcc 9166 with highest polygalacturonase activity was tested for utilization of raw pectins for enzyme production. polygalacturonase production was high in raw pectin source ... | 2010 | 23100850 |
| production and characterization of glucoamylase from fungus aspergillus awamori expressed in yeast saccharomyces cerevisiae using different carbon sources. | glucoamylase is widely used in the food industry to produce high glucose syrup, and also in fermentation processes for production beer and ethanol. in this work the productivity of the glucoamylase of aspergillus awamori expressed by the yeast saccharomyces cerevisiae, produced in submerged fermentation using different starches, was evaluated and characterized physico-chemically. the enzyme presented high specific activity, 13.8 u/mgprotein or 2.9 u/mgbiomass, after 48 h of fermentation using so ... | 2008 | 24031189 |
| three different types of α-amylases from aspergillus awamori kt-11: their purifications, properties, and specificities. | three forms of α-amylases, designated amyl i, amyl ii, and amyl iii were purified to a homogenous state by several column chromatographies from a koji culture in wheat bran of a strain of black mold, which was isolated in indonesia and identified as aspergillus awamori. they have molecular weights of 49,000, 63,000, and 97,000 by sds-page, respectively, and the optimum phs were 4.0 for amyl i and 5.5 for both amyl ii and amyl iii on soluble starch. amyl i hydrolyzed malto-tetraose, -pentaose, -h ... | 1998 | 27396998 |
| transxylosylation of β-xylosidase from aspergillus awamori k4. | β-xylosidase from aspergillus awamori k4 was purified. the optimum ph and temperature were around ph 4 and 70°c, and the molecular weight was estimated to be 117,000 on sds-page analysis. the enzyme has broad acceptor specificity in transxylosylation. especially, its acceptor accessibility for sorbitol and mannitol of sugar alcohols were higher than that for monosaccharides. trehalose was a much more effective acceptor than maltose and lactose of other disaccharides. in the reaction with 13-14% ... | 1997 | 27396877 |
| the mechanism of binding of glucoamylase i from aspergillus awamori var. kawachi to cyclodextrins and raw starch. | glucoamylase i (gai) bound to β-cyclodextrin (β-cd) with the binding constants of kd = 17.7 μm and n = 1.69. binding resulted in formation of an inclusion complex, as indicated by the fact that organic guest compounds for β-cd inhibited the binding of gai to β-cd. titration of gai (a(1)-r(615)) with β-cd or amylose a showed critical spectral perturbations at 286 nm that reflected the aromatic side chains of tyr and trp, but no spectral changes were observed in gai' (a(1)-v(469)) and gp-i (a(470) ... | 1994 | 27315704 |
| xylan-hydrolysing enzymes from thermophilic and mesophilic fungi. | screening of 40 mesophilic and 13 thermophilic fungi indicated that enzyme activities capable of degrading oat spelt xylan extensively were produced by only a few of the mesophilic species investigated. the relatively low degree of hydrolysis effected by the enzymes from thermophilic organisms could be explained, in part, by their lack of β-xylosidase. several strains of aspergillus awamori and aspergillus phoenicis were notable in producing high xylanase and β-xylosidase and low protease activi ... | 1991 | 24425133 |
| isolation of mutants of aspergillus awamori with enhanced production of extracellular xylanase and β-xylosidase. | plate screening tests were designed for the selection and isolation of mutant strains of the fungus aspergillus awamori cmi 142717 showing over-production and constitutive synthesis of xylanase and β-xylosidase. following mutation by n-methyl-n-nitro-n-nitrosoguanidine, nitrous acid and uv (254 nm), two generations of mutants were isolated and cultured in shake fiasks containing glucose, ball-milled oat straw or oat speit xylan as carbon source. growth of a number of selected mutants in shake fl ... | 1991 | 24425022 |
| bioactive profile, free-radical scavenging potential, dna damage protection activity, and mycochemicals in aspergillus awamori (mtcc 548) extracts: a novel report on filamentous fungi. | biomass of aspergillus awamori was investigated for mycochemicals, total phenolic compounds (tpc), condensed tannin content (ctc), free-radical scavenging potential (frsp), and dna damage protection activity. frsp was determined using dpph, abts, frap (ferric reducing antioxidant power), metal chelating activity, and cupric reducing antioxidant capacity) assays. water (aq), aqueous ethanol 50% (aqe), and methanol were used as extraction phase at 44.5 °c for 23.8 min. aqe shows the presence of ma ... | 2017 | 28660460 |
| the role of soybean extracts and isoflavones in hormone-dependent breast cancer: aromatase activity and biological effects. | estrogen receptor-positive (er(+)) breast cancer is the most common cause of cancer death in women worldwide. nowadays, the relationship between soya diet and breast cancer is controversial due to the unknown role of its isoflavones, genistein (g) and daidzein (d). in this work, we investigated not only the anti-tumor properties of a soybean extract (nbse) but also whether the biotransformation of extract (bse) by the fungus aspergillus awamori increased its effectiveness. the bse showed a stron ... | 2017 | 28644496 |
| biochemical characterization of the xylan hydrolysis profile of the extracellular endo-xylanase from geobacillus thermodenitrificans t12. | endo-xylanases are essential in degrading hemicellulose of various lignocellulosic substrates. hemicellulose degradation by geobacillus spp. is facilitated by the hemicellulose utilization (hus) locus that is present in most strains belonging to this genus. as part of the hus locus, the xyna gene encoding an extracellular endo-xylanase is one of the few secreted enzymes and considered to be the key enzyme to initiate hemicellulose degradation. several geobacillus endo-xylanases have been charact ... | 2017 | 28521816 |
| enhanced biodiesel production through phyco-myco co-cultivation of chlorella minutissima and aspergillus awamori: an integrated approach. | algae-fungus co-culture was investigated as an alternative biodiesel feedstock. an oleaginous filamentous fungus aspergillus awamori was co-cultured with chlorella minutissima mcc 27 and chlorella minutissima utex 2219, respectively in n11 medium furnished with different carbon and nitrogen sources. the biomass and lipid production potential of the two c. minutissima-a. awamori co-cultures was compared against the monocultures. a substantial enhancement in biomass and lipid accumulation was obse ... | 2017 | 28475992 |
| production, immobilization and thermodynamic studies of free and immobilized aspergillus awamori amylase. | enzyme cost, stability and its thermodynamic characteristics are the main criteria for industrial use. in this study, aspergillus awamori amylase was constitutively produced using various agro-industrial wastes. olive oil cake gave the highest activity (230u/g). the amylase was partially purified to 2.81-fold purification. immobilization was achieved using different carriers by covalent binding. the novel carrier ca(+2) alginate (alg) starch (st)/polyethyleneimine (pei)/glutaraldehyde (ga), show ... | 2017 | 28438682 |