| recognition of candida albicans als3 by the germ tube-specific monoclonal antibody 3d9.3. | monoclonal antibody 3d9.3 (mab 3d9.3) reacts with the surface of candida albicans germ tubes and recognizes a protein epitope. we used a two-step chromatography procedure to purify and identify the antigen (3d9) from c. albicans strain 66396 germ tubes. mab 3d9.3 recognized two intense protein bands at 140 and 180 kda. a comparative analysis between theoretical and experimental mass spectrum peaks showed that both bands corresponded to als3. this conclusion was supported by lack of reactivity be ... | 2009 | 19291169 |
| [identification of three candida africana strains in senegal]. | the frequency of candidiasis has increased dramatically in recent years. candida albicans is the most common species. however, other species which are pathogenic and resistant to usual antifungal agents beginning to emerge. these include candida dubliniensis and candida africana, which share morphological similarities with candida albicans. thus, it is of interest to correctly identify the fungal isolates. | 2012 | 23518168 |
| experimental pathogenicity of candida albicans and candida dubliniensis with continuous and discontinuous fringes morphotypes. | summary a possible correlation between the presence of discontinuous fringes and high virulence has been previously suggested. the aim of this study was to compare the pathogenicity of candida albicans and candida dubliniensis with continuous and discontinuous fringes morphotypes on mice. for c. albicans, two discontinuous fringe morphotype isolates (pn 69, pn 74), two continuous fringe morphotype isolates (n 60, n 33) and one reference strain were used. for c. dubliniensis, three discontinuous ... | 2010 | 20337935 |
| hydroxyurea therapy in sickle cell anemia patients aids to maintain oral fungal colonization balance. | the aim of this study was to evaluate the frequency of candida species and presence of lesions in the oral cavity of patients with sickle cell anemia (ss). | 2012 | 23278631 |
| real-time pcr analysis of fungal organisms and bacterial species at peri-implantitis sites. | the potential role of fungal organisms and their co-aggregation with either periodontopathogens or opportunistic pathogens at peri-implantitis sites is unknown. the aim of the present study was to qualitatively/quantitatively analyze and correlate fungal organisms and bacterial species at peri-implantitis sites. | 2015 | 27747631 |
| first isolation of candida dubliniensis from oral cavities of dermatological patients in nanjing, china. | candida dubliniensis is an emerging pathogen capable of causing both superficial and systemic infections. although c. dubliniensis and c. albicans are phenotypically similar, the two species differ in terms of epidemiology and the ability to rapidly develop resistance to fluconazole. c. dubliniensis is primarily associated with oral candidiasis of human immunodeficiency virus (hiv)-infected individuals. in this study, we describe the first recovery of c. dubliniensis from oral cavities of non-hi ... | 2011 | 21769632 |
| prevalence of periodontopathogens and candida spp. in smokers after nonsurgical periodontal therapy - a pilot study. | this pilot study aimed to evaluate the influence of smoking on clinical and microbiological parameters after nonsurgical periodontal therapy. forty-eight subjects were grouped into smokers (sm, n = 24) and nonsmokers (ns, n = 24) and paired according to gender, age, ethnicity, and periodontal status. both groups received oral hygiene education and scaling and root planing. clinical evaluation was performed using plaque index (pi), bleeding on probing (bop), pocket probing depth (ppd), gingival r ... | 2016 | 27556680 |
| in vitro antimicrobial and antiproliferative activity of amphipterygium adstringens. | amphipterygium adstringens is a plant widely used in mexican traditional medicine for its known anti-inflammatory and antiulcer properties. in this work, we evaluated the in vitro antimicrobial and antiproliferative activities of the methanolic extract of a. adstringens against oral pathogens such as streptococcus mutans, porphyromonas gingivalis, aggregatibacter actinomycetemcomitans, candida albicans, and candida dubliniensis, using microdilution (mic) and agar diffusion methods (mbc), and the ... | 2015 | 26451151 |
| prevalence of candida dubliniensis fungemia in argentina: identification by a novel multiplex pcr and comparison of different phenotypic methods. | candida dubliniensis is an emerging pathogen that can cause invasive disease in patients who have a variety of clinical conditions. c. dubliniensis is often misidentified as candida albicans by clinical laboratories. in argentina, incidence data are still scarce, and only one systemic infection has been reported. this study aims to determine the prevalence of c. dubliniensis in blood samples in argentina, to evaluate a novel pcr multiplex as well as several phenotypic methods for the identificat ... | 2011 | 21750939 |
| activity of a long-acting echinocandin (cd101) and seven comparator antifungal agents tested against a global collection of contemporary invasive fungal isolates in the sentry 2014 antifungal surveillance program. | the activity of cd101 and comparator antifungal agents against 606 invasive fungal isolates collected worldwide during 2014 was evaluated using the clinical and laboratory standards institute (clsi) method. all candida albicans (n = 251), candida tropicalis (n = 51), candida krusei (n = 16), and candida dubliniensis (n = 11) isolates were inhibited by ≤0.12 μg/ml of cd101 and were susceptible or showed wild-type susceptibility to the other echinocandins tested. five c. glabrata isolates (n = 100 ... | 2017 | 28052853 |
| fungal epidemiology and diversity in cystic fibrosis patients over a 5-year period in a national reference center. | the knowledge on prevalence rates of yeasts and moulds in patients with cystic fibrosis (cf) in germany is scarce. the aim of this report is to give an overview of the diversity and epidemiology of fungal species in cf patients. over a 5-year period, all fungal isolates cultured from microbiological specimen from cf patients were recorded. beside standard bacteriological culture media two fungal media were used for cultivation. species were identified by microscopy, biochemical profiling, maldi- ... | 2016 | 27364649 |
| antifungal susceptibilities of candida, cryptococcus neoformans and aspergillus fumigatus from the asia and western pacific region: data from the sentry antifungal surveillance program (2010-2012). | the sentry antifungal surveillance program monitors global susceptibility rates of newer and established antifungal agents. we report the in vitro activity of seven antifungal agents against 496 contemporary clinical isolates of yeasts and molds. the isolates were obtained from 20 laboratories in the asia-western pacific (apac) region during 2010 through 2012. anidulafungin, caspofungin, micafungin, fluconazole, itraconazole, posaconazole and voriconazole were susceptibility tested using clsi me ... | 2015 | 25899126 |
| comparative analysis of calcineurin signaling between candida dubliniensis and candida albicans. | candida dubliniensis, an emerging fungal pathogen, is the closest known species to the established pathogenic species candida albicans. despite the fact that these two species share > 80% genome sequence identity, they exhibit distinct properties such as less hyphal growth, reduced pathogenicity and increased sensitivity to sodium stress and elevated temperatures in c. dubliniensis compared with c. albicans. it is, however, largely unknown whether signaling pathways are conserved in the two cand ... | 2012 | 22808313 |
| synthesis of 1h-1,2,3-triazoles and study of their antifungal and cytotoxicity activities. | we report herein the results of antifungal activity of fifteen 1,2,3-triazoles against candida albicans, candida krusei, candida parapsilosis, candida kefyr, candida tropicalis, candida dubliniensis, tricophyton rubrum, microporum canis and aspergillus niger. all of the 1,2,3-triazoles were prepared from 1,3-dipolar cyclizations between aryl azides and alkynes catalyzed by cu(i), and several of the compounds exhibited antifungal activity with low cytotoxicity. the results demonstrated the potent ... | 2013 | 23432315 |
| prevalence of candida albicans, candida dubliniensis and candida africana in pregnant women suffering from vulvovaginal candidiasis in argentina. | vulvovaginal candidiasis (vvc) is a vulvovaginitis commonly diagnosed in gynecology care. in recent years, the taxonomy of the most important pathogenic candida species, such as candida albicans have undergone significant changes. | 2017 | 28385421 |
| population structure and molecular genetic characterization of 5-flucytosine-susceptible and -resistant clinical candida dubliniensis isolates from kuwait. | candida dubliniensis and candida albicans are two closely related species. although c. dubliniensis is less pathogenic, it has a higher propensity to develop resistance to fluconazole and some strains exhibit intrinsic resistance to 5-flucytosine (5-fc). all 5-fc-resistant isolates from kuwait were previously shown to belong to one of seven internal transcribed spacer (its) region of rdna-based haplotypes. this study performed fingerprinting of c. dubliniensis isolates by multilocus sequence typ ... | 2017 | 28380072 |
| antifungal activity of synthetic antiseptics and natural compounds against candida dubliniensis before and after in vitro fluconazole exposure. | this study evaluated the susceptibilities of oral candidiasis-derived candida albicans, fluconazole-resistant (fr) candida dubliniensis, and fluconazole-susceptible (fs) c. dubliniensis to synthetic antiseptics [chlorhexidine gluconate (chx), cetylpyridinium chloride (cpc), and triclosan (trc)] and natural compounds (carvacrol, eugenol and thymol). | 2017 | 28327805 |
| prevalence and antifungal drug sensitivity of non-albicans candida in oral rinse samples of self-caring elderly. | gerodontology 2010; doi: 10.1111/j.1741-2358.2010.00407.x prevalence and antifungal drug sensitivity of non-albicans candida in oral rinse samples of self-caring elderly aim: to assess the prevalence and antifungal drug sensitivity of non-albicans candida (nac) species in elderly outpatients. materials and methods: we investigated oral rinse samples of 194 self-caring elderly population (mean age 83 years) with emphasis on background factors for harbouring nac. susceptibility of candida species ... | 2010 | 20609005 |
| differential filamentation of candida albicans and candida dubliniensis is governed by nutrient regulation of ume6 expression. | candida dubliniensis is closely related to candida albicans; however, it is responsible for fewer infections in humans and is less virulent in animal models of infection. c. dubliniensis forms fewer hyphae in vivo, and this may contribute to its reduced virulence. in this study we show that, unlike c. albicans, c. dubliniensis fails to form hyphae in yeast extract-peptone-dextrose (ypd) medium supplemented with 10% (vol/vol) fetal calf serum (ypds medium). however, c. dubliniensis filaments in w ... | 2010 | 20639413 |
| in vitro activity of fluconazole and voriconazole against clinical isolates of candida spp. by e-test method. | the in vitro susceptibility of clinical candida isolates towards fluconazole and voriconazole was determined using the e-test method. a total of 41 clinical isolates recovered from patients since 2004 until 2009 from two local hospitals in kuala lumpur, malaysia were used. these comprised candida tropicalis, candida albicans, candida krusei, candida parapsilosis, candida rugosa, candida dubliniensis and candida glabrata. strains from american type culture collection were used as quality control. ... | 2010 | 20962716 |
| recovery of candida dubliniensis and other candida species from the oral cavity of subjects with periodontitis who had well-controlled and poorly controlled type 2 diabetes: a pilot study. | the goal of this study was to determine the prevalence of candida dubliniensis and other candida species from saliva samples and from subgingival plaque samples at periodontally healthy and periodontally diseased sites in subjects who had type 2 diabetes and periodontitis. saliva and subgingival samples were obtained from 30 subjects with periodontitis: 15 with poorly controlled and 15 with well-controlled type 2 diabetes. samples were analyzed for the presence of c. dubliniensis and other candi ... | 2010 | 21044102 |
| effect of marine polyunsaturated fatty acids on biofilm formation of candida albicans and candida dubliniensis. | the effect of marine polyunsaturated fatty acids on biofilm formation by the human pathogens candida albicans and candida dubliniensis was investigated. it was found that stearidonic acid (18:4 n-3), eicosapentaenoic acid (20:5 n-3), docosapentaenoic acid (22:5 n-3) and docosahexaenoic acid (22:6 n-3) have an inhibitory effect on mitochondrial metabolism of both c. albicans and c. dubliniensis and that the production of biofilm biomass by c. dubliniensis was more susceptible to these fatty acids ... | 2010 | 21116408 |
| differential virulence of candida albicans and c. dubliniensis: a role for tor1 kinase? | candida albicans and candida dubliniensis are two very closely related species of pathogenic yeast. c. albicans is the most prevalent species in the human gastrointestinal tract and is responsible for far more opportunistic infections in comparison with c. dubliniensis. this disparity is likely to be due to the reduced ability of c. dubliniensis to undergo the yeast to hypha transition, a change in morphology that plays an important role in c. albicans virulence. we have recently shown that hyph ... | 2011 | 21289475 |
| application of hypertonic sabouraud glucose agar for differentiation of candida dubliniensis from candida albicans. | an evaluation of hypertonic sabouraud glucose agar (sga) with 6.5% nacl for phenotypic differentiation of candida dubliniensis from candida albicans is reported. identity of the test fungi (c. albicans, 84; c. dubliniensis, 18) was based on their typical phenotypic characteristics and confirmed by a diagnostic polymerase chain reaction that targets the novel c. dubliniensis group i intron in the large ribosomal subunit. at 96 h of incubation at 28 -¦c, all of the 84 c. albicans isolates showed g ... | 2011 | 21396542 |
| presence of candida spp. in the oral cavity of heart transplantation patients. | candida spp. can lead to infections or even fungal sepsis particularly among immunocompromised individuals. | 2011 | 21437462 |
| prevalence of candida dubliniensis among oral candida isolates in patients attending the kuwait university dental clinic. | the aim of this study was to determine the oral candidal carriage of patients seeking dental treatment at the kuwait university dental clinic and to ascertain the candida species composition among them. | 2011 | 21454999 |
| identification of a second pad1 in brettanomyces bruxellensis lamap2480. | volatile phenols are aromatic compounds produced by some yeasts of the genus brettanomyces as defense against the toxicity of hydroxycinnamic acids (p-coumaric acid, ferulic acid and caffeic acid). the origin of these compounds in winemaking involves the sequential action of two enzymes: coumarate decarboxylase and vinylphenol reductase. the first one converts hydroxycinnamic acids into hydroxystyrenes, which are then reduced to ethyl derivatives by vinylphenol reductase. volatile phenols derive ... | 2017 | 27771809 |
| prevalence of candida albicans and candida dubliniensis in caries-free and caries-active children in relation to the oral microbiota-a clinical study. | the correlation between caries and the oral prevalence of candida spp. in children is contradictory in literature. thereby, authors focused on candida albicans as the most isolated candida species from the oral cavity. therefore, the aim of the present study was to compare caries-free and caries-bearing children regarding their oral carriage of candida spp. | 2016 | 26696116 |
| cdr2p contributes to fluconazole resistance in candida dubliniensis clinical isolates. | the development of resistance to azole antifungals used in the treatment of fungal infections can be a serious medical problem. here, we investigate the molecular mechanisms associated with reduced susceptibility to fluconazole in clinical isolates of candida dubliniensis , showing evidence of the trailing growth phenomenon. the changes in membrane sterol composition were studied in the presence of subinhibitory fluconazole concentrations. despite lanosterol and eburicol accumulating as the most ... | 2011 | 21542785 |
| comparative in vitro susceptibility of clinical isolates of candida paparsilosis complex and other candida species to caspofungin and anidulafungin by etest. | in vitro susceptibility of 141 clinical isolates of candida species to caspofungin and anidulafungin is reported. the etest was performed according to recommended procedure and minimum inhibitory concentrations (mics) were read after 24 h of incubation at 35°c. applying a breakpoint of <2 mg/ml, all candida spp. isolates, except those belonging to c. parapsilosis complex, were susceptible. the geometric mean for caspofungin and anidulafungin for different candida spp. were as follows: candida pa ... | 2011 | 21571626 |
| anabolic steroids affect human periodontal health and microbiota. | this study aims to evaluate periodontal microbiological differences between systemically healthy nonsmoker males taking anabolic androgenic steroids (aass) and non-aas users and to find associations between disease severity and aas use. | 2014 | 24221579 |
| susceptibility of candida albicans and candida dubliniensis to erythrosine- and led-mediated photodynamic therapy. | the effect of erythrosine- and led-mediated photodynamic therapy (pdt) on planktonic cultures and biofilms of candida albicans and candida dubliniensis was evaluated. planktonic cultures of standardized suspensions (10(6)cells/ml) of c. albicans and c. dubliniensis were treated with erythrosine concentrations of 0.39-200++m and leds in a 96-well microtiter plate. biofilms formed by c. albicans and c. dubliniensis in the bottom of a 96-well microtiter plate were treated with 400++m erythrosine an ... | 2011 | 21704304 |
| activation of mapk/c-fos induced responses in oral epithelial cells is specific to candida albicans and candida dubliniensis hyphae. | oral epithelial cells detect the human pathogenic fungus candida albicans via nf-+¦b and a bi-phasic mitogen-activated protein kinase (mapk) signaling response. however, discrimination between c. albicans yeast and hyphal forms is mediated only by the mapk pathway, which constitutes activation of the mapk phosphatase mkp1 and the c-fos transcription factor and is targeted against the hyphal form. given that c. albicans is not the only candida species capable of filamentation or causing mucosal i ... | 2011 | 21706283 |
| identification of local clinical candida isolates using chromagar candida™ as a primary identification method for various candida species. | the objective of our study was to study the effectiveness of chromagar candida™ as the primary identification method for various clinical candida isolates, other than the three suggested species by the manufacturer. we studied 34 clinical isolates which were isolated from patients in a local teaching hospital and 7 atcc strains. these strains were first cultured in sabouraud dextrose broth (sdb) for 36 hours at 35ºc, then on chromagar plates at 30ºc, 35ºc and 37ºc. the sensitivity of t ... | 2011 | 22041745 |
| the gray phenotype and tristable phenotypic transitions in the human fungal pathogen candida tropicalis. | phenotypic plasticity, the ability to switch between different morphological types, plays critical roles in environmental adaptation, leading to infections, and allowing for sexual reproduction in pathogenic candida species. candida tropicalis, which is both an emerging human fungal pathogen and an environmental fungus, can switch between two heritable cell types termed white and opaque. in this study, we report the discovery of a novel phenotype in c. tropicalis, named the gray phenotype. simil ... | 2016 | 27246518 |
| variation in biofilm formation among blood and oral isolates of candida albicans and candida dubliniensis. | biofilm production is considered a potential virulence factor of some candida species. for this reason, an understanding of biofilm behavior of candida albicans and its closely related species candida dubliniensis is key to the development of effective preventive measures for invasive and oral candidiasis. the aim of this study was to compare the capacity of biofilm production by blood and oral isolates of c. albicans and c. dubliniensis using polystyrene, flat-bottomed 100-well microtiter plate ... | 2011 | 21899928 |
| development of candida-specific real-time pcr assays for the detection and identification of eight medically important candida species. | culture-based identification methods have been the gold standard for the diagnosis of fungal infection. currently, molecular technologies such as real-time pcr assays with short turnaround time can provide desirable alternatives for the rapid detection of candida microbes. however, most of the published pcr primer sets are not candida specific and likely to amplify dna from common environmental contaminants, such as aspergillus microbes. in this study, we designed pan-candida primer sets based o ... | 2016 | 27103821 |
| evaluation of matrix-assisted laser desorption ionization-time-of-flight mass spectrometry to differentiate between candida albicans and candida dubliniensis. | matrix-assisted laser desorption ionization-time-of-flight mass spectrometry (maldi-tof-ms) analysis in conjunction with the direct formic acid (fa) sample processing method was evaluated for the ability to differentiate the closely related species of candida albicans and candida dubliniensis. the results showed that maldi-tof-ms, using the direct fa method, was reliable to differentiate between these species. | 2016 | 26971641 |
| production of virulence factors in candida strains isolated from patients with denture stomatitis and control individuals. | the aim of this study was to evaluate the production of virulence factors in candida isolates from the oral cavities of 50 patients with different degrees of denture stomatitis (ds, type i, ii and iii) and 50 individuals without signs of ds. we evaluated the enzymatic and hemolytic activities, the biofilm formation, and the cell surface hydrophobicity (csh) in all isolates. germ tube (gt) production was also evaluated in candida albicans and candida dubliniensis isolates. in c. albicans and c. d ... | 2016 | 26971635 |
| a simple xylose-based agar medium for the differentiation of candida dubliniensis and candida albicans. | the utility of xylose-based agar medium for differentiation of candida dubliniensis from candida albicans is evaluated. all c. dubliniensis isolates failed to grow on this medium, while c. albicans isolates yielded good growth. this simple in-house medium offers an inexpensive alternative to commercial yeast identification systems for resource poor settings. | 2011 | 22209683 |
| influence of doxorubicin on fluconazole susceptibility and efflux pump gene expression of candida dubliniensis. | the effect of doxorubicin (dox) on the fluconazole (flu) susceptibility of c. dubliniensis was investigated. isolates were exposed to dox and flu in a chequerboard assay and resistance gene expressions were analysed after dox exposure. the susceptibility of the yeast to flu was decreased in the presence of dox in the chequerboard assay with fic indices suggesting an antagonistic effect. gene expression analyses showed an overexpression of cdcdr2. hence, dox was found to have an impact on resista ... | 2011 | 21939345 |
| distribution of yeast species associated with oral lesions in hiv-infected patients in southwest uganda. | oropharyngeal candidiasis remains a significant clinical problem in hiv-infected and aids patients in regions of africa where anti-retroviral therapy isn't readily available. in this study we identified the yeast populations associated with oral lesions in hiv-infected patients in southwest uganda who were receiving treatment with nystatin and topical clotrimazole. samples were taken from 605 patients and 316 (52%) of these yielded yeast growth following incubation on sabouraud dextrose agar. sa ... | 2011 | 21905950 |
| gray phenotype: enhanced fitness strategy for candida dubliniensis? | | 2016 | 26786843 |
| determination of antifungal susceptibility patterns among the clinical isolates of candida species. | candida species are opportunistic yeasts that cause infections ranging from simple dermatosis to potentially life-threatening fungemia. the emergence of resistance to antifungal drugs has been increased in the past two decades. | 2011 | 22223999 |
| the first documented case of candida dubliniensis leptomeningeal disease in an immunocompetent host. | in june 2008, a 25-year-old man presented to the royal adelaide hospital, australia, with candida dubliniensis leptomeningeal disease. on examination the patient had focal neurological deficits, both sensory and motor. he tested negative for hiv and had normal t cell subsets. the patient was treated with 6 weeks of intravenous liposomal amphotericin b and oral flucytosine, and had a full recovery. | 2011 | 22687683 |
| an in vitro study of antifungal drug susceptibility of candida species isolated from human immunodeficiency virus seropositive and human immunodeficiency virus seronegative individuals in lucknow population uttar pradesh. | candidiasis is the most common opportunistic infection in human immunodeficiency virus (hiv) seropositive patients, starting from asymptomatic colonization to pathogenic forms and gradual colonization of non-albicans in patients with advanced immunosuppression leads to resistance for azole group of antifungal drugs with high rate of morbidity and mortality. | 2017 | 26604498 |
| oral infections caused by yeasts in patients with head and neck cancer undergoing radiotherapy. identification of the yeasts and evaluation of their antifungal susceptibility. | yeasts occur as part of the normal human microbiota. nevertheless, some species are opportunistic, affecting immunocompromised patients such as those undergoing oncologic treatment. | 2012 | 23518170 |
| comparison of cell wall proteins in putative candida albicans & candida dubliniensis by using modified staining method & sds-page. | candida species are among the most common causes of opportunistic fungal diseases. among candida species, candida albicans is responsible for most infections. having many strains, c. albicans is very polymorph. c. dubliniensis is very similar to albicans species both morphologically and physiologically. for an infection to occur, cell wall proteins play an important role as they enable yeast to adhere to host cells and begin pathogenesis. therefore, we decided to extract these proteins and exami ... | 2012 | 23482280 |
| 7-hydroxycalamenene effects on secreted aspartic proteases activity and biofilm formation of candida spp. | the 7-hydroxycalamenenene-rich essential oil (eo) obtained from the leaves of croton cajucara (red morphotype) have been described as active against bacteria, protozoa, and fungi species. in this work, we aimed to evaluate the effectiveness of 7-hydroxycalamenenene against candida albicans and nonalbicans species. | 2016 | 27019560 |
| mechanisms involved in the inhibition of glycolysis by cyanide and antimycin a in candida albicans and its reversal by hydrogen peroxide. a common feature in candida species. | in candida albicans, cyanide and antimycin a inhibited k(+) transport, not only with ethanol-o2 as the substrate, but also with glucose. the reason for this was that they inhibited not only respiration, but also fermentation, decreasing atp production. measurements of oxygen levels in cell suspensions allowed identification of the electron pathways involved. nadh fluorescence levels increased in the presence of the inhibitors, indirectly indicating lower levels of nad(+) and so pointing to glyce ... | 2015 | 26363023 |
| enlightening the role of subdomains in the catalytic behavior of lipases/acyltransferases homologous to cplip2 through rational design of chimeric enzymes. | the lipases/acyltransferases homologous to cplip2 of candida parapsilosis catalyze efficiently acyltransfer reactions in lipid/water media with high water activity (aw > 0.9). the characterization of two new enzymes of this family, cdulac from candida dubliniensis and callac8 from candida albicans, was performed. despite their 82% identity, these 2 enzymes have significant differences in their catalytic behavior. to understand the role played by the different subdomains of these proteins (the ma ... | 2017 | 28258600 |
| [cancer and mycoses and literature review]. | various infectious agents are classical risk factors for cancer including bacteria, viruses and parasites. there is less evidence concerning the implication of fungal infection in carcinogenesis. the role of chronic candida infection in the development of squamous cell carcinoma has been suspected for years. candida sp are more prevalent in potentially malignant disorder and cancer of the oral mucosa. other epidemiological evidence of a link between candida infection and cancer is what is observ ... | 2017 | 28188605 |
| [evaluation of pna-fish method for direct identification of candida species in blood culture samples and its potential impact on guidance of antifungal therapy]. | early antifungal therapy has a major influence on survival in candidemia. rapid identification of the species has importance for the treatment, prediction of the species-specific primary resistance and variable antifungal susceptibility. recently, molecular-based methods attempt to reduce the time between the positive signal of a blood culture and identification of the fungus. pna-fish (peptide nucleic acid fluorescence in situ hybridization) assay distinguishes a number of frequently isolated c ... | 2016 | 28124963 |
| csr1/zap1 maintains zinc homeostasis and influences virulence in candida dubliniensis but is not coupled to morphogenesis. | the supply and intracellular homeostasis of trace metals are essential for every living organism. therefore, the struggle for micronutrients between a pathogen and its host is an important determinant in the infection process. in this work, we focus on the acquisition of zinc by candida dubliniensis, an emerging pathogen closely related to candida albicans. we show that the transcription factor csr1 is essential for c. dubliniensis to regulate zinc uptake mechanisms under zinc limitation: it gov ... | 2015 | 26002718 |
| candida survival strategies. | only few candida species, e.g., candida albicans, candida glabrata, candida dubliniensis, and candida parapsilosis, are successful colonizers of a human host. under certain circumstances these species can cause infections ranging from superficial to life-threatening disseminated candidiasis. the success of c. albicans, the most prevalent and best studied candida species, as both commensal and human pathogen depends on its genetic, biochemical, and morphological flexibility which facilitates adap ... | 2015 | 25911234 |
| in vitro antifungal susceptibility of oral candida isolates from patients suffering from caries and chronic periodontitis. | caries and chronic periodontitis are common oral diseases where a higher candida colonization is reported. antifungal agents could be adjuvant drugs for the therapy of both clinical conditions. the aim of the current study has been to evaluate the in vitro activities of conventional and new antifungal drugs against oral candida isolates from patients suffering from caries and/or chronic periodontitis. in vitro activities of amphotericin b, fluconazole, itraconazole, miconazole, nystatin, posacon ... | 2017 | 28124220 |
| cytotoxic and antimicrobial effects of indium(iii) complexes with 2-acetylpyridine-derived thiosemicarbazones. | complexes [in(2ac4oclph)cl2(meoh)] (1), [in(2ac4pfph)cl2(meoh)] (2), [in(2ac4pclph)cl2(meoh)] (3) and [in(2ac4piph)cl2(meoh)] (4) were obtained with n(4)-ortho-chlorophenyl-2-acetylpyridine thiosemicarbazone (h2ac4oclph), n(4)-para-fluorophenyl-2-acetylpyridine thiosemicarbazone (h2ac4pfph), n(4)-para-chlorophenyl-2-acetylpyridine thiosemicarbazone (h2ac4pclph) and n(4)-para-iodophenyl-2-acetylpyridine thiosemicarbazone (h2ac4piph). theoretical studies suggested that the coordinated methanol mol ... | 2017 | 28009892 |
| hematogenous dissemination of candida dubliniensis causing spondylodiscitis and spinal abscess in a hiv-1 and hcv-coinfected patient. | we report a case of spondylodiscitis and spinal abscess following haematogenous dissemination of the emerging yeast candida dubliniensis in a human immunodeficiency virus-1 (hiv-1) and hepatitis c virus (hcv)-coinfected patient. although c. dubliniensis is considered less virulent compared to its closest known relative candida albicans, reports of severe fungal infections are increasing. this case indicates that the pathogenicity of c. dubliniensis may be higher than previously believed. therefo ... | 2015 | 25750857 |
| can serums be replaced by mueller-hinton agar in germ tube test? | the germ tube test (gtt) is inexpensive, easy, and well-defined test that differentiates candida albicans (excluding candida dubliniensis and candida africana) from other species. the aim of this study was to evaluate various serums (i.e., human, rabbit, horse, and fetal bovine serum) used in the gtt and mueller-hinton agar (mha). | 2017 | 27958248 |
| susceptibility of candida albicans and candida dubliniensis to photodynamic therapy using four dyes as the photosensitizer. | oral candidiasis is the most common opportunistic infection affecting the human oral cavity. photodynamic therapy, as one of its proposed treatment modalities, needs a distinct dye for achieving the best effect. | 2016 | 27942552 |
| candida species from oral cavity of hiv-infected children exhibit reduced virulence factors in the haart era. | this study aimed to assess, in vitro, the biofilm viability and the phospholipase and protease production of candida spp. from the saliva of hiv infected children and healthy controls, and to correlate the results with the use of medical data. a total of 79 isolates were analyzed: 48 candida albicans isolates (33/15) and 20 candida parapsilosis sensu lato complex isolates (12/8) (from hiv/control patients, respectively), and 8 candida krusei, 1 candida tropicalis, 1 candida dubliniensis and 1 ca ... | 2017 | 27913142 |
| echinocandin resistance in candida species isolates from liver transplant recipients. | liver transplant recipients are at risk of invasive fungal infections, especially candidiasis. echinocandin is recommended as prophylactic treatment but is increasingly associated with resistance. our aim was to assess echinocandin drug resistance in candida spp. isolated from liver transplant recipients treated with this antifungal class. for this, all liver-transplanted patients in a university hospital (créteil, france) between january and june of 2013 and 2015 were included. susceptibilities ... | 2017 | 27855078 |
| chemical composition and antimicrobial evaluation of the essential oils of bocageopsis pleiosperma maas. | essential oils from the leaves, twigs and barks of bocageopsis pleiosperma maas were obtained by using hydrodistillation and analysed by using gas chromatography coupled to mass spectrometry. several compounds (51) were detected and identified, being β-bisabolene the main component in all aerial parts of the plant, with higher concentration in the leaves (55.77%), followed by barks (38.53%) and twigs (34.37%). in order to increase the biological knowledge about the essential oil of bocageopsis s ... | 2015 | 25562370 |
| [yeast species in vulvovaginitis candidosa]. | vulvovaginal candidiasis is the most common mycosis, however, the available information about antifungal susceptibilities of these yeasts is limited. | 2015 | 25544052 |
| candida species diversity and antifungal susceptibility patterns in oral samples of hiv/aids patients in baja california, mexico. | candidiasis is the most common opportunistic fungal infection in hiv patients. the aims of this study were to identify the prevalence of carriers of candida, candida species diversity, and in vitro susceptibility to antifungal drugs. in 297 hiv/aids patients in baja california, mexico, candida strains were identified by molecular methods (pcr-rflp) from isolates of oral rinses of patients in tijuana, mexicali, and ensenada. 56.3% of patients were colonized or infected with candida in tijuana, th ... | 2016 | 27630251 |
| investigation of minor species candida africana, candida stellatoidea and candida dubliniensis in the candida albicans complex among yaoundé (cameroon) hiv-infected patients. | minor species of the candida albicans complex may cause overestimation of the epidemiology of c. albicans, and misidentifications could mask their implication in human pathology. authors determined the occurrence of minor species of the c. albicans complex (c. africana, c. dubliniensis and c. stellatoidea) among yaoundé hiv-infected patients, cameroon. stool, vaginal discharge, urine and oropharyngeal samples were analysed by mycological diagnosis. isolates were identified by conventional method ... | 2015 | 25289589 |
| postantifungal effect of caspofungin against the candida albicans and candida parapsilosis clades. | killing and postantifungal effects could be relevant for the selection of optimal dosing schedules. this study aims to compare time-kill and postantifungal effects with caspofungin on candida albicans (c. albicans, candida dubliniensis, candida africana) and candida parapsilosis (c. parapsilosis, candida metapsilosis, candida orthopsilosis) clades. in the postantifungal effect experiments, strains were exposed to caspofungin for 1 h at concentrations 0.12-8 μg/ml. time-kill experiments were cond ... | 2016 | 27492134 |
| in vitro antifungal activity of different components of centratherum anthelminticum and ocimum sanctum seed oils and their synergism against oral pathogenic fungi. | background. opportunistic fungal infections like candidiasis are common in the oral cavity. in recent years candida species have shown resistance against a number of synthetic drugs. this study assessed the antifungal activity of centratherum anthelminticum and ocimum sanctum seed oils against six common pathogenic candida strains. synergistic activity of the major oil components was also studied. methods. antifungal activity of centratherum anthelminticum and ocimum sanctum seed oils were teste ... | 2016 | 27429725 |
| epidemiologic and microbiologic evaluation of nosocomial infections associated with candida spp in children: a multicenter study from istanbul, turkey. | the purpose of this study was to establish species distribution of candida isolates from pediatric patients in istanbul, turkey, and to determine risk factors associated with nosocomial candida infections. | 2016 | 27339794 |
| oral microbial colonization in children with sickle cell anaemia under long-term prophylaxis with penicillin. | sickle cell anaemia (sca) is the most frequent haematological hereditary disease. children with sca are submitted to long-term prophylactic therapy with penicillin, but little is known about its impact on oral microflora. the aim of this study was to evaluate the oral microbial colonization of paediatric patients with sca. | 2014 | 24967510 |
| phylogenetic and syntenic analyses of the 12-spanner drug:h(+) antiporter family 1 (dha1) in pathogenic candida species: evolution of mdr1 and flu1 genes. | candida albicans and other pathogenic candida species can develop resistance to clinical fungicides through active drug export mediated by multidrug efflux pumps, in particular by members of the drug:h(+) antiporter family 1 (dha1). the dha1 proteins encoded in the genomes of 31 hemiascomycetous strains from 25 species were identified and homology relationships between these proteins and the functionally characterised dha1 in the model yeast saccharomyces cerevisiae were established. gene neighb ... | 2014 | 24907457 |
| vapour and liquid-phase artemisia annua essential oil activities against several clinical strains of candida. | candida spp. are often the cause of infection in immune-compromised individuals. they are characterized by a strong resistance to antimicrobial drugs and disinfectants. the activity of artemisia annua essential oil against candida spp. was determined by vapour contact and microdilution assay. the oil was characterized by the presence of oxygenated monoterpenes (more than 75 % of the constituents), mainly represented by the irregular monoterpene artemisia ketone (ca. 22 %), and the widespread mon ... | 2016 | 27286334 |
| multi-drug resistant oral candida species isolated from hiv-positive patients in south africa and cameroon. | candida species are a common cause of infection in immune-compromised hiv-positive individuals, who are usually treated with the antifungal drug, fluconazole, in public hospitals in africa. however, information about the prevalence of drug resistance to fluconazole and other antifungal agents on candida species is very limited. this study examined 128 candida isolates from south africa and 126 cameroonian candida isolates for determination of species prevalence and antifungal drug susceptibility ... | 2014 | 24726686 |
| species distribution and antifungal susceptibility profile of candida isolates from bloodstream infections in lima, peru. | yeast identification and in vitro susceptibility testing provide helpful information for appropriate administration of antifungal treatments; however, few reports from the latin american region have been published. the aim of this study was to identify the species present in isolates from bloodstream infections diagnosed in nine hospitals in lima, peru and to determine their in vitro susceptibility to four antifungal drugs. we tested and identified 153 isolates collected between october 2009 and ... | 2014 | 24667770 |
| association of clinical and demographic factors in invasive candidiasis caused by fluconazole-resistant candida species: a study in 15 hospitals, medellín, colombia 2010-2011. | candida is the most important agent of fungal infections. several risk factors have been described associated with invasive infection by fluconazole-resistant candida spp. a prospective cross-sectional study with case-control analysis was conducted. case group patients with fluconazole-resistant candida isolate were included; control group were patients with fluconazole-susceptible candida spp. a multivariate logistic regression model was performed. three hundred isolates of candida spp. were an ... | 2014 | 24666706 |
| etiologic agents and antifungal susceptibility of oral candidosis from romanian patients with hiv-infection or type 1 diabetes mellitus. | this is the first romanian investigation of oral candidosis in patients suffering of hiv-infection or type 1 diabetes mellitus (t1dm). candida albicans was the dominant species in both types of isolates: n = 14 (46.7%) in t1dm, n = 60 (69.8%) in hiv. the most frequent non-albicans candida spp. were candida kefyr (n = 6; 20%) in t1dm and candida dubliniensis (n = 8; 9.3%) in hiv. resistance to fluconazole was detected only in the hiv non-albicans candida group (n = 8; 9.3%). all isolates were sus ... | 2016 | 27282005 |
| a substituted sulfonamide and its co (ii), cu (ii), and zn (ii) complexes as potential antifungal agents. | a sulfonamide 1-tosyl-1-h-benzo(d)imidazol-2-amine (tbza) and three new complexes of co(ii), cu(ii), and zn(ii) have been synthesized. the compounds have been characterized by elemental analyses, ftir, (1)h, and (13)c-nmr spectroscopy. the structure of the tbza, and its co(ii) and cu(ii) complexes, was determined by x-ray diffraction methods. tbza and its co(ii) complex crystallize in the triclinic p-1 space group, while the cu(ii) complex crystallizes in the monoclinic p21/c space group. antifu ... | 2016 | 27232977 |
| oral candida carriage and immune status in thai human immunodeficiency virus-infected individuals. | oral candidiasis is a common opportunistic infection among human immunodeficiency virus (hiv)-infected individuals, with growing concerns about the emergence of non-albicans species with resistance to antifungal agents. this cross-sectional study determined the prevalence of oral candida species in thai hiv-infected adults and factors affecting their colonization. candida species were identified from oral rinse samples of 60 hiv-infected participants of the mtct-plus initiative and 49 healthy co ... | 2014 | 24591706 |
| prevalence and antifungal susceptibility of candida albicans and its related species candida dubliniensis and candida africana isolated from vulvovaginal samples in a hospital of argentina. | candida africana taxonomical status is controversial. it was proposed as a separate species within the candida albicans species complex; however, phylogenetic analyses suggested that it is an unusual variety of c. albicans. the prevalence of c. albicans-related species (candida dubliniensis and c. africana) as vulvovaginal pathogens is not known in argentina. moreover, data on antifungal susceptibility of isolates causing vulvovaginal candidiasis is scarce. the aims of this study were to establi ... | 2016 | 26922471 |
| fluconazole and amphotericin-b resistance are associated with increased catalase and superoxide dismutase activity in candida albicans and candida dubliniensis. | candida dubliniensis, a new species of candida that has been recovered from several sites in healthy people, has been associated with recurrent episodes of oral candidiasis in aids and hiv-positive patients. this species is closely related to c. albicans. the enzymatic activity of c. dubliniensis in response to oxidative stress is of interest for the development of drugs to combat c. dubliniensis. | 2014 | 24474018 |
| paediatric oropharyngeal and cutaneous candidiasis with special reference to candida dubliniensis. | mucocutaneous and cutaneous candidiasis, though common in children, is often under-reported. the prevalence of candida dubliniensis in causing these infections in this age group is also largely unknown. a prospective epidemiological cross-sectional study for candidiasis was performed in paediatric patients clinically suspected of candidiasis with oropharyngeal lesions (75 patients), cutaneous lesions (18 patients) and lesions at both sites (2 patients). candida species were identified by convent ... | 2014 | 24445508 |
| caspofungin-induced in-vitro post-antifungal effect and its impact on adhesion related traits of oral candida dubliniensis and candida albicans isolates. | adhesion to buccal epithelial cells (bec) and denture acrylic surfaces (das), germ tube (gt) formation and cell surface hydrophobicity (csh) are all virulence traits involved in the pathogenicity of candida. post-antifungal effect (pafe) also have a bearing on pathogenicity and virulence of candida. candida dubliniensis is associated with oral and systemic candidosis, which can be managed with caspofungin. there is no published information on caspofungin-induced pafe and its impact on adhesion t ... | 2016 | 26850765 |
| repeat-associated fission yeast-like regional centromeres in the ascomycetous budding yeast candida tropicalis. | the centromere, on which kinetochore proteins assemble, ensures precise chromosome segregation. centromeres are largely specified by the histone h3 variant cenp-a (also known as cse4 in yeasts). structurally, centromere dna sequences are highly diverse in nature. however, the evolutionary consequence of these structural diversities on de novo cenp-a chromatin formation remains elusive. here, we report the identification of centromeres, as the binding sites of four evolutionarily conserved kineto ... | 2016 | 26845548 |
| in vitro activity of natural phenolic compounds against fluconazole-resistant candida species: a quantitative structure-activity relationship analysis. | to evaluate the antifungal activity and to analyse the structure-activity relationship of eleven natural phenolic compounds against four candida species which are resistant to fluconazole. | 2014 | 24387763 |
| antifungal susceptibility and growth inhibitory response of oral candida species to brucea javanica linn. extract. | candida species have been associated with the emergence of resistant strains towards selected antifungal agents. plant products have been used traditionally as alternative medicine to ease candidal infections. the present study was undertaken to investigate the antifungal susceptibility patterns and growth inhibiting effect of brucea javanica seeds extract against candida species. | 2013 | 24305010 |
| discovery of the gray phenotype and white-gray-opaque tristable phenotypic transitions in candida dubliniensis. | candida dubliniensis is closely related to candida albicans, a major causative agent of candidiasis, and is primarily associated with oral colonization and infection in human immunodeficiency virus (hiv)-positive patients. despite the high similarity of genomic and phenotypic features between the 2 species, c. dubliniensis is much less virulent and less prevalent than c. albicans. the ability to change morphological phenotypes is a striking feature of candida species and is linked to virulence. ... | 2016 | 26714067 |
| candida albicans exhibits enhanced alkaline and temperature induction of efg1-regulated transcripts relative to candida dubliniensis. | filamentous growth is an important virulence trait of the human pathogenic fungi within the genus candida, and the greater propensity of c. albicans to form hyphae has been proposed to account for the greater virulence of this species relative to the less pathogenic species c. dubliniensis. in this meta-analysis, we compare the transcriptional response of c. dubliniensis and c. albicans to the individual environmental stimuli that shape the gene expression profiles during filamentation in 10% se ... | 2015 | 26697354 |
| clinical isolates and laboratory reference candida species and strains have varying abilities to form biofilms. | candida biofilms are a major virulence trait for this yeast. in this study, the biofilm-forming ability of the major medically important clinical and laboratory reference strains was compared. biofilms were quantified using traditional methods, that is, crystal violet (cv), tetrazolium (xtt) reduction and colony-forming unit assays (cfu), and two new methods: an automated cell counter (acc) and biofilm suspension turbidity (bst) method. biofilms could be categorized based on biofilm biomass (hig ... | 2013 | 23927631 |
| chromatin immunoprecipitation (chip) assay in candida albicans. | chromatin immunoprecipitation (chip) is a widely used technique which can determine the in vivo association of a specific protein on a particular dna locus in the genome. in this method cross-linked chromatin is sheared and immunoprecipitated with antibodies raised against a target protein of interest. the end result of this process is the enrichment of dna fragments associated with the desired protein. thus, interactions between proteins and genomic loci in cellular context can be determined by ... | 2016 | 26519064 |
| characterising atypical candida albicans clinical isolates from six third-level hospitals in bogotá, colombia. | candida species are the most frequently found fungal pathogens causing nosocomial disease in a hospital setting. such species must be correctly identified to ensure that appropriate control measures are taken and that suitable treatment is given for each species. candida albicans is causing most fungal disease burden worldwide; the challenge lies in differentiating it from emerging atypical, minor and related species such as candida dubliniensis and candida africana. the purpose of this study wa ... | 2015 | 26438104 |
| postantifungal effect of micafungin against the species complexes of candida albicans and candida parapsilosis. | micafungin is an effective antifungal agent useful for the therapy of invasive candidiasis. candida albicans is the most common cause of invasive candidiasis; however, infections due to non-c. albicans species, such as candida parapsilosis, are rising. killing and postantifungal effects (pafe) are important factors in both dose interval choice and infection outcome. the aim of this study was to determinate the micafungin pafe against 7 c. albicans strains, 5 candida dubliniensis, 2 candida afric ... | 2015 | 26168269 |
| [evaluation of common commercial systems for the identification of yeast isolates in microbiology laboratories: a multicenter study]. | accurate and rapid identification of yeast isolates have become important in recent years for not only antifungal susceptibility testing due to the species-specific clinical resistance breakpoints but also early initiation of appropriate antifungal therapy. in clinical microbiology laboratories species identification of yeasts is often performed with several commercial systems based on biochemical properties and rarely according to the physiological and morphological characteristics. the aim of ... | 2015 | 26167821 |
| phenotypic detection of genitourinary candidiasis among sexually transmitted disease clinic attendees in ladoke akintola university teaching hospital, osogbo, nigeria. | the management of genitourinary candidiasis (gc) is fraught with challenges, especially, in an era of increasing antifungal resistance. this descriptive cross-sectional study conducted between may 2013 and january 2014 determined the prevalence and characteristics of gc and the species of candida among 369 attendees of a sexually transmitted disease (std) clinic of ladoke akintola university teaching hospital, osogbo, nigeria. appropriate urogenital specimen collected from each attendee was exam ... | 2015 | 26064140 |
| species and condition specific adaptation of the transcriptional landscapes in candida albicans and candida dubliniensis. | although candida albicans and candida dubliniensis are most closely related, both species behave significantly different with respect to morphogenesis and virulence. in order to gain further insight into the divergent routes for morphogenetic adaptation in both species, we investigated qualitative along with quantitative differences in the transcriptomes of both organisms by cdna deep sequencing. | 2013 | 23547856 |
| species distribution and virulence factors of candida spp. isolated from the oral cavity of kidney transplant recipients in brazil. | although yeasts belonging to the genus candida are frequently seen as commensals in the oral cavity, they possess virulence attributes that contribute for pathogenicity. the aims of the present study were to study the prevalence of candida spp. isolated from the oral cavity of renal transplant recipients and to analyze strains virulence factors. we isolated a total of 70 candida strains from 111 transplant recipients, and candida albicans was the most prevalent species (82.86 %). oral candidiasi ... | 2013 | 23539354 |
| discrimination of candida albicans from candida dubliniensis by use of the biofire filmarray blood culture identification panel. | | 2015 | 25878337 |
| in vitro anticandidal evaluation of novel highly functionalized bis cyclohexenone ethyl carboxylates. | novel highly functionalized bis cyclohexenone ethyl carboxylates 7-12 were designed, synthesized and their structures were elucidated by their elemental analysis, ms, ft-ir, one-dimensional 1h, and 13c nmr spectroscopic data. | 2013 | 23426531 |
| first-time isolation of candida dubliniensis from plaque and carious dentine of primary teeth. | to determine those organisms of the genus candida associated with dental caries by investigating samples from active carious lesions. within the genus candida, the species candida albicans and candida dubliniensis are capable of forming chlamydospores and germ tubes. until it became possible in 1995 to differentiate between the two species taxonomically, c. dubliniensis was falsely identified as c. albicans. whilst the importance of c. albicans for rapidly progressing early childhood caries (ecc ... | 2015 | 25800500 |
| comparison of the in vitro activity of echinocandins against candida albicans, candida dubliniensis, and candida africana by time-kill curves. | candida albicans remains the most common fungal pathogen. this species is closely related to 2 phenotypically similar cryptic species, candida dubliniensis and candida africana. this study aims to compare the antifungal activities of echinocandins against 7 c. albicans, 5 c. dubliniensis, and 2 c. africana strains by time-kill methodology. mic values were similar for the 3 species; however, differences in killing activity were observed among species, isolates, and echinocandins. echinocandins pr ... | 2015 | 25703894 |
| [endogenous candida dubliniensis endophtalmitis. first case in mexico]. | candida endophthalmitis is related to immunosuppression state, intravenous catheters, invasive procedures and parenteral feeding. it is estimated that between 2% and 10% of endophthalmitis are endogenous, within fungal etiology the most frequently isolated microorganism is candida albicans. the infection by c. dubliniensis is reported in less than 2 % of the cases of infection by candida at systemic level and few reported cases of endophthalmitis. the clinical presentation is poor vision , vitri ... | 2017 | 25680648 |