| lord byron's death: a case of late malarial relapse? | the study examines the pathological circumstances related to byron's death, the primary issue being malaria. lord byron died during the greek war of independence against the ottoman empire, in messolonghi on 19 april 1824. byron's medical profile consists of recurrent onsets of fever, which gave rise to the issue of malaria relapses. according to byron's letters he reported crises of fever in greece (1810), malta (1811), italy (1817-1819) and england. evidence from byron's autopsy, specifically ... | 2015 | 26397304 |
| [diagnosis of an imported case of plasmodium ovale infection in nanping city, fujian]. | an imported case previously misdiagnosed as vivax malaria was reviewed. the epidemiological data and blood sample were collected. the detection was conducted by microscopy, rapid diagnostic test (rdt) and nested pcr. the case was finally comfirmed as the first imported case of plasmodium ovale infection in nanping. | 2015 | 26080533 |
| [application of nested pcr in the diagnosis of imported plasmodium ovale infection]. | to identity plasmodium ovale infection by 18s rrna gene nested pcr. | 2015 | 26080528 |
| dimorphism in genes encoding sexual-stage proteins of plasmodium ovale curtisi and plasmodium ovale wallikeri. | plasmodium ovale curtisi and plasmodium ovale wallikeri are distinct species of malaria parasite which are sympatric throughout the tropics, except for the americas. despite this complete overlap in geographic range, these two species do not recombine. although morphologically very similar, the two taxa must possess distinct characters which prevent recombination between them. we hypothesised that proteins required for sexual reproduction have sufficiently diverged between the two species to pre ... | 2015 | 25817462 |
| do hypnozoites cause relapse in malaria? | the concept that hypnozoites give rise to relapses in plasmodium vivax and plasmodium ovale malaria has become dogma. however, it is evident from particular contemporary research findings that hypnozoites are not necessarily the origin of all relapse-like recurrences of malaria caused by these parasites. this is the core opinion presented, and i discuss it fully. the hypnozoite theory of relapse needs to be re-evaluated in view of the recent, increased focus on p. vivax and liver stages of plasm ... | 2015 | 25816801 |
| [iditification of five imported cases of plasmodium ovale wallikeri infection in zhejiang province]. | to identify and analyze plasmodium ovale wallikeri in 5 imported malaria cases, who were detected positive by microscopy and negative by conventional pcr. | 2014 | 25726600 |
| first imported plasmodium ovale malaria in central america: case report of a guatemalan soldier and a call to improve its accurate diagnosis. | the mesoamerican ministers of health have set 2020 as the target for malaria elimination to be achieved in the region. imported malaria cases are a potential threat to countries attempting elimination or working to prevent resurgence. we report the first imported plasmodium ovale infection with molecular confirmation in central america, which occurred in a guatemalan soldier that had been deployed in africa. the obstacles for its diagnosis using the standard microscopy technique and the need to ... | 2015 | 25722882 |
| establishing a china malaria diagnosis reference laboratory network for malaria elimination. | in china, the prevalence of malaria has reduced dramatically due to the elimination programme. the continued success of the programme will depend upon the accurate diagnosis of the disease in the laboratory. the basic requirements for this are a reliable malaria diagnosis laboratory network and quality management system to support case verification and source tracking. | 2015 | 25628071 |
| characterization of plasmodium ovale curtisi and p. ovale wallikeri in western kenya utilizing a novel species-specific real-time pcr assay. | plasmodium ovale is comprised of two genetically distinct subspecies, p. ovale curtisi and p. ovale wallikeri. although p. ovale subspecies are similar based on morphology and geographical distribution, allelic differences indicate that p. ovale curtisi and p. ovale wallikeri are genetically divergent. additionally, potential clinical and latency duration differences between p. ovale curtisi and p. ovale wallikeri demonstrate the need for investigation into the contribution of this neglected mal ... | 2015 | 25590587 |
| optimal primaquine use for radical cure of plasmodium vivax and plasmodium ovale malaria in japanese travelers--a retrospective analysis. | recently, a dose of 30 mg (base) primaquine daily for 14 days is increasingly recommended for radical cure of plasmodium vivax malaria. however, total primaquine doses, or those per body weight, are also recognized as important. in japan, primaquine is not a licensed medicine, but has been used through the research group on chemotherapy of tropical diseases for >3 decades. | 2015 | 25482427 |
| performance of rapid diagnostic tests for plasmodium ovale malaria in japanese travellers. | rapid diagnostic tests (rdts) are used widely in the diagnosis of malaria. although the effectiveness of rdts for malaria has been described in many previous studies, the low performance of rdt particularly for plasmodium ovale malaria in traveller has rarely been reported. | 2014 | 25473374 |
| [diagnosis of the first imported case of plasmodium ovale infection at guangdong port]. | the first imported case of plasmodium ovale infection in guangdong province was identified. the patient worked in myanmar for one week and had a fever when he arrived at guangzhou baiyun international airport. epidemiological information and blood sample were collected. the detection was conducted by microscopy, right view rapid malaria test (rdts) and real-time pcr with plasmodium genus-specific and species-specific primers and probes. the case showed weak positive rdt result, and was confirmed ... | 2014 | 25065219 |
| new type of ssurdna sequence was detected from both plasmodium ovale curtisi and plasmodium ovale wallikeri samples. | plasmodium ovale is relatively unfamiliar to chinese staff engaged in malaria diagnosis. in 2013, dried blood spots of four unidentified but suspected ovale malaria samples were sent to the national malaria reference laboratory (nmrl) for reconfirmation. | 2014 | 24893846 |
| [overviews on plasmodium ovale wallikeri and its genetic detection system]. | in recent years, some microscopy-positive but nest-pcr negative (based on small subunit ribosomal rna gene) ovale malaria cases were reported. the detection failure was due to the genetic variations between plasomdoium ovale wallikeri and p. ovale curtisi. this paper reviews the discovery and validation processes of p. ovale wallikeri and the detection systems for identification of p. ovale. | 2014 | 24822370 |
| [diagnosis and analysis of the first imported ovale malaria case in wenzhou city]. | the first imported case of plasmodium ovale infection in wenzhou city was confirmed by microscopy and pcr test. the patient returned from the people's republic of congo to wenzhou for three and a half months presented a history of fever with chills and rigors on april 30, 2012. the results from peripheral blood smear examination and pcr analysis confirmed a diagnosis of p. ovale infection. the patient was treated with chloroquine plus primaquine for eight days and the symptoms improved. | 2013 | 24818395 |
| [species identification in 5 imported cases previously diagnosed as vivax malaria by parasitological and nested pcr techniques]. | to identify the species of malaria parasites in 5 imported cases previously diagnosed as vivax malaria. | 2013 | 24812863 |
| [laboratory detection on two cases with imported plasmodium ovale infection]. | to compare the laboratory tests of the imported plasmodium ovale infection and analyse the genetic character. | 2013 | 24809194 |
| plasmodium ovale malaria in brazil: report of an imported case with a prolonged incubation period. | we report the first case of imported plasmodium ovale in brazil, confirmed using both conventional microscopy and pcr-based protocols. the patient was a 36-year-old brazilian male who had been working as a miner in cabinda province, angola. based on his travel history, the parasite was dormant for at least two years. the relatively long period of incubation of p. ovale may obscure the link between exposure and disease. the recent increase in the number of people travelling to regions where p. ov ... | 2014 | 24727525 |
| pharmacokinetic interactions between primaquine and chloroquine. | chloroquine combined with primaquine has been the standard radical curative regimen for plasmodium vivax and plasmodium ovale malaria for over half a century. in an open-label crossover pharmacokinetic study, 16 healthy volunteers (4 males and 12 females) aged 20 to 47 years were randomized into two groups of three sequential hospital admissions to receive a single oral dose of 30 mg (base) primaquine, 600 mg (base) chloroquine, and the two drugs together. the coadministration of the two drugs d ... | 2014 | 24687509 |
| comparison of imported plasmodium ovale curtisi and p. ovale wallikeri infections among patients in spain, 2005-2011. | sequencing data from plasmodium ovale genotypes co-circulating in multiple countries support the hypothesis that p. ovale curtisi and p. ovale wallikeri are 2 separate species. we conducted a multicenter, retrospective, comparative study in spain of 21 patients who had imported p. ovale curtisi infections and 14 who had imported p. ovale wallikeri infections confirmed by pcr and gene sequencing during june 2005-december 2011. the only significant finding was more severe thrombocytopenia among pa ... | 2014 | 24572501 |
| emergence of plasmodium ovale malaria among the french armed forces in the republic of ivory coast: 20 years of clinical and biological experience. | french military surveillance identified an increase in plasmodium ovale attacks among soldiers in ivory coast. this emergence and the low sensitivity of biological tests raise the question of a possible role of p. ovale variant species. | 2014 | 24429426 |
| a case of plasmodium ovale wallikeri infection in a chinese worker returning from west africa. | in contrast to the gradual reduction in the number of locally transmitted malaria cases in china, the number of imported malaria cases has been increasing since 2008. here, we report a case of a 39-year-old chinese man who acquired plasmodium ovale wallikeri infection while staying in ghana, west africa for 6 months in 2012. microscopic examinations of giemsa-stained thin and thick blood smears indicated plasmodium vivax infection. however, the results of rapid diagnostic tests, which were condu ... | 2013 | 24327782 |
| two cases of late plasmodium ovale presentation in military personnel. | only a few late cases of plasmodium ovale presentation have been reported in literature. we describe two cases of late p. ovale presentation occurring in two french military personnel, 47 and 69 months, respectively, after a stay in côte d'ivoire and in the absence of new intercurrent malaria exposure. | 2014 | 24205853 |
| warm autoimmune hemolytic anemia secondary to plasmodium ovale infection: a case report and review of the literature. | a three year old male from the democratic republic of the congo was admitted to monroe carell jr. children's hospital at vanderbilt with a 10-day history of fever, emesis, and diarrhea. examination demonstrated scleral icterus, splenomegaly, and anemia. by peripheral blood smear, the patient was diagnosed with plasmodium ovale. immunohematology demonstrated a positive direct antiglobulin test (dat) for igg and c3d with pan-agglutination on eluate. these findings, in combination with hemolytic la ... | 2013 | 24148713 |
| genetic marker suitable for identification and genotyping of plasmodium ovale curtisi and plasmodium ovale wallikeri. | we present a seminested pcr method that specifically discriminates between plasmodium ovale curtisi and p. ovale wallikeri with high sensitivity. the test is based on species-specific amplification of a size-polymorphic fragment of the tryptophan-rich antigen gene, potra, which also permits discrimination of intraspecific sequence variants at this locus. | 2013 | 24068009 |
| toxicological study of the primaquine phosphate loaded chitosan nanoparticles in mice. | primaquine (pq) shows activity against the late hepatic stages and latent tissue forms of plasmodium vivax and plasmodium ovale. however, liver targeted pq delivery may be useful to minimize the dose-limiting blood toxicities and side-effects of pq. the prime objective of this study was the preparation of pq loaded chitosan nanoparticles (pq-cs-nps) in order to enhance drug tolerance and to reduce dose frequency. the morphological analysis of the chitosan nps displayed particle size in the range ... | 2013 | 23973494 |
| [a case of plasmodium ovale malaria--morphological diagnostic difficulty and utility of rapid diagnostic tests]. | a 46-year-old japanese man was referred to our travel clinic because of high fever for the past 7 days. he worked as an engineer for a month in zambia and returned to japan 2 days ago. he had a high-grade fever of 40.5 degrees c. examination of the palpebral conjunctiva showed no evidence of anemia. liver and spleen were not palpable. blood sample was collected at the time of the febrile paroxysm. malaria parasites were detected by examination of giemsa-stained thin blood films. the dominant fea ... | 2013 | 23672079 |
| primaquine for radical cure of plasmodium vivax and plasmodium ovale malaria: an observational survey (2008-2010). | since 2008, the french guidelines have promoted the systematic use of 30 mg/day of primaquine for the radical cure of plasmodium vivax and plamodium ovale infections. we observed three relapses in 10 patients with p vivax acquired in french guiana. no relapses were seen in west african p ovale patients. | 2014 | 23464724 |
| [laboratory testing for a case of imported plasmodium ovale infection in zhejiang province]. | blood sample obtained from a patient, which returned from equatorial guinea, with clinical diagnosis of plasmodium infection was confirmed as imported p. ovale infection by etiology and molecular biological methods. 50 microl blood was obtained before taking anti-malarial drugs to make thin and thick blood smears, giemsa stained, and observed by microscopy. genomic dna was extracted from the blood sample, and detected for dna fragment of p. ovale, p. vivax, p. falciparum or p. malariae by real-t ... | 2013 | 24812847 |
| a new real-time pcr for the detection of plasmodium ovale wallikeri. | it has been proposed that ovale malaria in humans is caused by two closely related but distinct species of malaria parasites: p. ovale curtisi and p. ovale wallikeri. we have extended and optimized a real-time pcr assay targeting the parasite's small subunit ribosomal rna (ssrrna) gene to detect both these species. when the assay was applied to 31 archival blood samples from patients diagnosed with p. ovale, it was found that the infection in 20 was due to p. ovale curtisi and in the remaining 1 ... | 2012 | 23110165 |
| [the vivax malaria causative agent plasmodium ovale: the present global area, intraspecies polymorphism, importation to the russian federation (1992-2011)]. | the global area for plasmodium ovale is small as compared with that for other species of human malaria pathogens. it has expanded in asian areas and remained as before in the african ones. in the past 20 years, there have been 2129 malaria cases imported from far abroad to russia, including 84 (4%) cases of vivax malaria (p. ovale). the patients were most foreign citizens: 70 from 20 african countries and 7 from two countries of oceania, such as papua new guinea and indonesia. the other 7 patien ... | 2012 | 23088152 |
| two techniques for simultaneous identification of plasmodium ovale curtisi and plasmodium ovale wallikeri by use of the small-subunit rrna gene. | the primers traditionally used to detect plasmodium ovale infections are known for not binding all p. ovale parasites within the small-subunit rrna gene when used alone. we describe a simple, cost- and time-efficient multiplex nested pcr and a nested pcr using a novel set of primers for the simultaneous detection of p. ovale curtisi and p. ovale wallikeri. | 2012 | 23015675 |
| plasmodium ovale in bangladesh: genetic diversity and the first known evidence of the sympatric distribution of plasmodium ovale curtisi and plasmodium ovale wallikeri in southern asia. | in spite of the high prevalence of malaria in bangladesh and other southern asian countries, there remains a substantial shortage of knowledge about the less common human malaria parasites. recent studies indicate that plasmodium ovale is made up of two species, namely plasmodium ovale wallikeri and plasmodium ovale curtisi. genus- and species-specific nested pcr analyses of the ssrrna gene was used to detect p. ovale infections among 2,246 diagnostic samples. plasmodium ovale infections were fu ... | 2012 | 22633951 |
| 'not another uti ... '. | malaria includes a global disease burden with approximately 300-500 million cases worldwide annually. varied symptomatology creates a diagnostic challenge. this is a case report of a stroke rehabilitation facility resident who developed plasmodium ovale infection, several months post-exposure. physicians should maintain a broad list of differential diagnoses, thinking beyond the range of common diagnoses. | 2012 | 22417982 |
| the first case of plasmodium ovale malaria from bangladesh. | the authors report the first indigenous case of plasmodium ovale infection from bangladesh. the diagnosis was confirmed by pcr and sequence analysis. the patient had neither been outside of the country nor ever received blood transfusions. the authors concluded that there was evidence for a local transmission of p ovale malaria in bangladesh. p ovale malaria should therefore always be considered a potential differential diagnosis in the indigenous population as well as travellers and migrants re ... | 2010 | 22778371 |
| malaria in the guangxi zhuang autonomous region in china: a twelve-year surveillance data study. | the incidence of an indigenous malaria, defined as malaria acquired by a local mosquito transmission, declined from 2004 to 2015 in the guangxi zhuang autonomous region. however, imported malaria, defined as malaria acquired from other endemic regions outside of china, has been increasing in the region, as in the rest of the country, particularly the disease caused by plasmodium falciparum. a retrospective study was conducted to explore malaria-endemic characteristics in guangxi during the 2004- ... | 2017 | 28820683 |
| cryptic plasmodium ovale concurrent with mixed plasmodium falciparum and plasmodium malariae infection in two children from central african republic. | since several malaria parasite species are usually present in a particular area, co-infections with more than one species of plasmodium are more likely to occur in humans infected in these areas. in many mixed infections, parasite densities of the cryptic species may be low and often not recognized in clinical practice. | 2017 | 28810865 |
| non-falciparum malaria imported mainly from africa: a review from a portuguese hospital. | non-falciparum malaria (nfm) has been reported to be responsible for around 25% of imported malaria cases in europe but is often neglected due to its less severe clinical course when compared to plasmodium falciparum. differentiation between species is however crucial for a correct approach. the objective of this study is to report the cases of this often missed aetiology of malaria in a tertiary hospital in portugal. | 2017 | 28743266 |
| a dual colour fluorescence in situ hybridization (fish) assay for identifying the zoonotic malaria parasite plasmodium knowlesi with a potential application for the specific diagnosis of knowlesi malaria in peripheral-level laboratories of southeast asia. | plasmodium knowlesi is primarily responsible for zoonotic malaria in several southeast asian countries. precise identification of the parasite in the blood of patients presently relies on an expensive and elaborate pcr procedure because microscopic examination of blood and other available field identification techniques lack adequate specificity. therefore, the use of a simple and inexpensive dual-colour fluorescence in situ hybridization (fish) assay, analogous to fish assays recently described ... | 2017 | 28724402 |
| [biological disturbances affecting people with malaria: about thirty cases]. | | 2017 | 28674567 |
| imported malaria in pregnant women experienced in japan. | with ever-growing global exchanges, the number of travelers, including pregnant women, to the tropics is increasing, which poses a risk of contracting malaria. although there are several reports on imported malaria in pregnancy from western countries, those focusing on cases experienced in japan are very limited. | 2017 | 28583709 |
| malaria pathogenesis. | in the mosquito-human life cycle, the six species of malaria parasites infecting humans (plasmodium falciparum, plasmodium vivax, plasmodium ovale wallickeri, plasmodium ovale curtisi, plasmodium malariae, and plasmodium knowlesi) undergo 10 or more morphological states, replicate from single to 10,000+ cells, and vary in total population from one to many more than 10(6) organisms. in the human host, only a small number of these morphological stages lead to clinical disease and the vast majority ... | 2017 | 28533315 |
| evaluation of the utility value of three diagnostic methods in the detection of malaria parasites in endemic area. | malaria is a debilitating disease with high morbidity and mortality in africa, commonly caused by different species of the genus plasmodium in humans. misdiagnosis is a major challenge in endemic areas because of other disease complications and technical expertise of the medical laboratory staff. microscopic method using giemsa-stained blood film has been the mainstay of diagnosis of malaria. however, since 1993 when rapid diagnostic test (rdt) kits were introduced, they have proved to be effect ... | 2017 | 28477621 |
| nested multiplex pcr for identification and detection of human plasmodium species including plasmodium knowlesi. | to develop a new technique for diagnosis of plasmodium knowlesi and at the same time to be able to discriminate among the diverse species of plasmodium causing human malaria. | 2017 | 28442114 |
| metal-dna interactions improve signal in high-resolution melting of dna for species differentiation of plasmodium parasite. | the success of high-resolution melting (hrm) analysis for distinguishing similar dnas with minor base mismatch differences is limited. here, metal-mediated structural change in dna has been exploited to amplify hrm signals leading to differentiation of target dnas in an orthologous gene corresponding to four plasmodium species. conserved 26-mer ssdnas from ldh gene of the four plasmodium species were employed as targets. a capture probe (cp) that is fully complementary to the plasmodium falcipar ... | 2017 | 28421327 |
| [role of primaquine in malaria control and elimination in french-speaking africa]. | primaquine, an 8-aminoquinoline, is a relatively unknown and underutilized drug in french-speaking african countries. it acts against the liver stage parasites of all human malaria species, asexual blood stages of plasmodium vivax and, to a lesser degree, plasmodium falciparum; p. falciparum mature gametocytes, and p. vivax and plasmodium ovale hypnozoites. gastrointestinal disturbances are its most common side effects. the clinical utility of primaquine is limited due to its hematological side ... | 2017 | 28417346 |
| molecular diagnosis of plasmodium ovale by photo-induced electron transfer fluorogenic primers: pet-pcr. | accurate diagnosis of malaria infections continues to be challenging and elusive, especially in the detection of submicroscopic infections. developing new malaria diagnostic tools that are sensitive enough to detect low-level infections, user friendly, cost effective and capable of performing large scale diagnosis, remains critical. we have designed novel self-quenching photo-induced electron transfer (pet) fluorogenic primers for the detection of p. ovale by real-time pcr. in our study, a total ... | 2017 | 28640824 |
| molecular and immunological analyses of confirmed plasmodium vivax relapse episodes. | relapse infections resulting from the activation hypnozoites produced by plasmodium vivax and plasmodium ovale represent an important obstacle to the successful control of these species. a single licensed drug, primaquine is available to eliminate these liver dormant forms. to date, investigations of vivax relapse infections have been few in number. | 2017 | 28558712 |
| [spontaneous spleen rupture with p. ovale malaria]. | | 2017 | 28545674 |
| case report of plasmodium ovale curtisi malaria in sri lanka: relevance for the maintenance of elimination status. | following its recent certification as malaria-free, imported infections now pose the greatest threat for maintaining this status in sri lanka. imported infections may also introduce species that are uncommon or not previously endemic to these areas. we highlight in this case report the increasing importance of less common malaria species such as plasmodium ovale in elimination settings and discuss its relevance for the risk of malaria resurgence in the country. | 2017 | 28438137 |