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bacteria dispersal by hitchhiking on zooplankton.microorganisms and zooplankton are both important components of aquatic food webs. although both inhabit the same environment, they are often regarded as separate functional units that are indirectly connected through nutrient cycling and trophic cascade. however, research on pathogenic and nonpathogenic bacteria has shown that direct association with zooplankton has significant influences on the bacteria's physiology and ecology. we used stratified migration columns to study vertical dispersal ...201020547852
population-level effects of spinosad and bacillus thuringiensis israelensis in daphnia pulex and daphnia magna: comparison of laboratory and field microcosm exposure conditions.because exposure to toxicants not only results in mortality but also in multiple sublethal effects, the use of life-table data appears particularly suitable to assess global effects on exposed populations. the present study uses a life table response approach to assess population-level effects of two insecticides used against mosquito larvae, spinosad (8 μg/l) and bacillus thuringiensis var. israelensis (bti, 0.5 μl/l), on two non target species, daphnia pulex and daphnia magna (crustacea: clado ...201020552396
ecotoxicity and genotoxicity assessment of cytotoxic antineoplastic drugs and their metabolites.in spite of growing scientific concern about pharmaceuticals in the environment, there is still a lack of information especially with regard to their metabolites. the present study investigated ecotoxicity and genotoxicity of three widely used cytostatic agents 5-fluorouracil (5-fu), cytarabine (cyt) and gemcitabine (gemc) and their major human metabolites, i.e. alpha-fluoro-beta-alanine (fbal), uracil-1-beta-d-arabinofuranoside (arau) and 2',2'-difluorodeoxyuridine (dfdu), respectively. effects ...201020624627
aquatic multi-species acute toxicity of (chlorinated) anilines: experimental versus predicted data.aquatic toxicity information is essential in environmental risk assessment to determine the potential hazards and risks of new and existing chemicals. prediction and modelling techniques, such as quantitative structure activity relationships (qsar) and species sensitivity distributions (ssds), are applied to fill data gaps and to predict, assess and extrapolate the toxicity of chemicals. in this study, both techniques (i.e. the ecosar programme as qsar tool and ssds) were assessed for a set of p ...201020637490
treatment of phenol-contaminated soil by corynebacterium glutamicum and toxicity removal evaluation.biodegradation of phenol-contaminated soils using corynebacterium glutamicum, was optimized in this study. phenol degradation by c. glutamicum was observed in soil supplemented with 1% yeast extract as a substrate. we determined the optimal inoculation size of c. glutamicum (7.4 log(10) cfu ml(-1)) for the degradation of phenol-contaminated soil. under optimal conditions (1% yeast extract and 7.4 log(10) cfu ml(-1) of c. glutamicum), the efficiency of phenol degradation in soil was greater than ...201020638173
fitness and virulence of a bacterial endoparasite in an environmentally stressed crustacean host.host-parasite interactions are shaped by the co-evolutionary arms race of parasite virulence, transmission success as well as host resistance and recovery. the virulence and fitness of parasites may depend on host condition, which is mediated, for instance, by host energy constraints. here, we investigated to what extent stress imposed by predation threat and environmental pollutants influences host-parasite interactions. we challenged the crustacean host daphnia magna with the sterilizing bacte ...201120663250
interspecies correlations of toxicity to eight aquatic organisms: theoretical considerations.interspecies correlations allow the prediction of toxicity to a number of other species. however, little attention has been paid to the theoretical considerations of the interspecies relationship based on the differences of bio-uptake and toxic mechanism between species. this study examines the interspecies correlations of toxicity between species of vibrio fischeri, river bacteria, algae, daphnia magna, carp, tetrahymena pyriformis, fathead minnow and guppy based on the theoretical background. ...201020673582
evaluation of the aquatic toxicity of two veterinary sulfonamides using five test organisms.the aquatic toxicity of sulfaquinoxaline (sqo) and sulfaguanidine (sgd) was evaluated on the following test organisms: daphnia magna (reproduction test), pseudokirchneriella subcapitata, scenedesmus dimorphus, synecococcus leopoliensis (algal growth inhibition test) and lemna gibba (duckweed growth inhibition test). furthermore, the additivity of the two compounds was measured on d. magna (acute immobilisation test) and p. subcapitata (algal growth inhibition test) using the isobologram method. ...201020673955
introduction of oxygenated side chain into imidazolium ionic liquids: evaluation of the effects at different biological organization levels.the biological effects of a class of oxygenated imidazolium ionic liquids were studied in comparison with alkyl imidazolium salts (bmim bf4 and bmim n(cn)2).the cellular and subcellular effects were evaluated on rat pheochromocytoma pc12 cell lines, through mtt test, lactate dehydrogenase release and acetylcholinesterase inhibition; the eco-toxicological responses were assessed through the acute toxicity tests towards daphnia magna and vibrio fischeri. the introduction of ethoxy moieties in the ...201020674022
phylogenetic position of octosporea muscaedomesticae (microsporidia) and its relationship to octosporea bayeri based on small subunit rdna analysis.comparative phylogenetic analysis of the small subunit rdna sequence of octosporea muscaedomesticae (flu, 1911) (type species) (microsporidia) isolated from the blowfly phormia regina (diptera:calliphoridae) is presented. neighbor joining bootstrap, maximum parsimony and maximum likelihood analyses with 38 microsporidian taxa representing five major clades of microsporidia placed o. muscaedomesticae on a separate branch within a clade containing parasites of freshwater hosts. o. muscaedomesticae ...201020688077
first report on interspecies quantitative correlation of ecotoxicity of pharmaceuticals.pharmaceuticals being extensively and progressively used in human and veterinary medicine are emerging as significant environmental contaminants. pharmaceuticals are designed to have a specific mode of action and many of them are persistent in the body. these features among others make pharmaceuticals to be evaluated for potential effects on aquatic flora and fauna. low levels of pharmaceuticals have been detected in many countries in sewage treatment plant effluents, surface waters, groundwater ...201020692010
age related acute effects of microcystin-lr on daphnia magna biotransformation and oxidative stress.as a result of eutrophication among other factors, bloom forming species of cyanobacteria frequently thrive and dominate communities in freshwater ecosystems. cyanobacteria are considered as a nuisance and hazardous due to the production of toxins. the most potent and studied cyanobacterial toxins are the microcystins, especially the variant microcystin-lr (mc-lr). daphnia magna plays a central role in freshwater pelagic food webs and its populations are negatively affected by cyanobacteria due ...201020692276
quantitative concentration-toxicity relationship for the injury of rat thymocytes by chemical compounds used in inter-laboratory toxicity ring-tests.rat thymic lymphocytes were used to determine the cytotoxic effect of the two toxicants, 3,4-dichloroaniline (dca) and sodium bromide (nabr), at concentrations that have effects in the 21-day reproduction test with daphnia magna. to evaluate the effect of the chemical compounds on cell membrane integrity, we measured the permeability of the thymocytes to trypan blue and the leakage of lactate dehydrogenase (ldh) from the cells. the viability and metabolic activity of the thymocytes were quantifi ...199420693023
phenoloxidase but not lytic activity reflects resistance against pasteuria ramosa in daphnia magna.the field of ecological immunology strongly relies on indicators of immunocompetence. two major indicators in invertebrates, the activity of phenoloxidase (po) and lytic activity have recently been questioned in studies showing that, across a natural range of baseline levels, these indicators did not predict resistance against a manipulated challenge with natural parasites. we confirmed this finding by showing that baseline levels of po and lytic activity in the host daphnia magna were not relat ...201120810432
the chronic toxicity of molybdate to freshwater organisms. i. generating reliable effects data.the european union regulation on registration, evaluation, authorization and restriction of chemical substances (reach) (ec, 2006) requires the characterization of the chronic toxicity of many chemicals in the aquatic environment, including molybdate (moo(4)(2-)). our literature review on the ecotoxicity of molybdate revealed that a limited amount of reliable chronic no observed effect concentrations (noecs) for the derivation of a predicted no-effect concentration (pnec) existed. this paper pre ...201020813395
structural effects of the bioavailable fraction of pesticides in soil: suitability of elutriate testing.this study focused the ecotoxicological evaluation of four different pesticides (chlorpyrifos, glyphosate, vinclozolin, endosulfan), sprayed into an agricultural soil, using a standard battery of aquatic bioassays for testing of soil elutriates: vibrio fischeri -microtox(®); pseudokirchneriella subcapitata growth inhibition; daphnia magna acute and chronic toxicity. despite relevant pesticide residues were recovered from the soil matrix (concentrations higher than 1000 μg kg(-1)), much lower con ...201020817396
toxicity of organic pollutants to seven aquatic organisms: effect of polarity and ionization.the toxicity of organic chemicals to vibrio fischeri, river bacteria, algae, daphnia magna and fishes were analysed. the results showed that the toxicity of chemicals to narcotics was dependent on hydrophobicity. a single model for both polar and non-polar narcotics was developed by inclusion of a polarity descriptor as well as the hydrophobic parameter. the highly hydrophobic polar narcotics could be treated as non-polar narcotics because their polar functional group(s) make(s) a relatively sma ...201020818578
toxic hazard of leachates from furfurylated wood: comparison between two different aquatic organisms.environmental concern regarding the use of toxic preservatives such as chromated copper arsenate (cca) has been put forward. in the european union, united states, and japan, cca has been phased out for residential and water-contact applications. ecotoxicological studies of wood treated with conventional preservatives were carried out in the late 1990s, and it was concluded that the main impact is to water and aquatic organisms. today, alternatives to conventional preservation methods, marketed a ...201020821541
comparative toxicity and bioconcentration of nonylphenol in freshwater organisms.degradation of alkylphenol ethoxylates to more persistent alkylphenols such as nonylphenol occurs in wastewater treatment plants where nonylphenol is released to aquatic systems. in this study, acute and chronic tests were conducted to determine the toxicity and bioconcentration of nonylphenol to freshwater organisms for use in deriving national water quality criteria. acute median effect concentrations (ec50s) based on loss of equilibrium, immobility, and lethality for species representing seve ...201020821669
toxicity assessment of sediments from three european river basins using a sediment contact test battery.the toxicity of four polluted sediments and their corresponding reference sediments from three european river basins were investigated using a battery of six sediment contact tests representing three different trophic levels. the tests included were chronic tests with the oligochaete lumbriculus variegatus, the nematode caenorhabditis elegans and the mudsnail potamopyrgus antipodarum, a sub-chronic test with the midge chironomus riparius, an early life stage test with the zebra fish danio rerio, ...201120833427
non-target organism effects tests on vip3a and their application to the ecological risk assessment for cultivation of mir162 maize.transgenic crops producing insecticidal proteins from bacillus thuringiensis (bt) provide economic, environmental and health benefits by maintaining or increasing crop yields with fewer applications of insecticide. to sustain these benefits, it is important to delay the evolution of insect resistance to the proteins, and to ensure that the proteins do not harm non-target organisms, particularly those that may control secondary pests that would otherwise flourish because of reduced insecticide ap ...201120839052
effects of binary mixtures on the life traits of daphnia magna.the environment is constantly exposed to a cocktail of contaminants mainly due to human activities. because polluted ecosystems are characterized by an amalgam of chemical compounds, the objective of the present study was to assess the joint effect of chemical mixtures to the life--history traits of daphnia magna straus. for that a binary mixture of two neonicotinoid insecticides, imidacloprid and thiacloprid, and another one of imidacloprid with nickel chloride were tested. theoretical models h ...201120843551
an assessment of the potential of the microbial assay for risk assessment (mara) for ecotoxicological testing.rapid microscale toxicity tests make it possible to screen large numbers of compounds and greatly simplify toxicity identification evaluation and other effect directed chemical analyses of effluents or environmental samples. tests using vibrio fischeri (such as microtox®) detect toxicants that cause non-specific narcosis, but are insensitive to other important classes of contaminants. the microbial assay for risk assessment (mara) is a 24 h multi-species test that seeks to address this problem b ...201020882341
cytological and molecular description of hamiltosporidium tvaerminnensis gen. et sp. nov., a microsporidian parasite of daphnia magna, and establishment of hamiltosporidium magnivora comb. nov.we describe the new microsporidium hamiltosporidium tvaerminnensis gen. et sp. nov. with an emphasis on its ultrastructural characteristics and phylogenetic position as inferred from the sequence data of ssu rdna, alpha- and beta-tubulin. this parasite was previously identified as octosporea bayeri jírovec, 1936 and has become a model system to study the ecology, epidemiology, evolution and genomics of microsporidia - host interactions. here, we present evidence that shows its differences from o ...201020946698
relationships between acute toxicities of para nitrophenol (p-np) and nitrobenzene (nb) to daphnia magna and photobacterium phosphoreum: physicochemical properties and metabolites under anaerobic/aerobic sequentials.in this study, the acute toxicities of nitrobenzene (nb) and para nitrophenol (p-np) were investigated in a high rate sequential anaerobic migrating blanket (ambr)/aerobic completely stirred tank reactor (cstr) using microtox and daphnia magna tests. after sequential anaerobic and aerobic treatments, the inhibitions in the microtox bacteria decreased from an initial 78.10-48.20% and 4.00%, respectively, in wastewater containing 40.00 mg/l p-np. the inhibitions of the influent wastewater containi ...201021035948
β-cyclocitral, a grazer defence signal unique to the cyanobacterium microcystis.β-cyclocitral is often present in eutrophic waters and is a well known source of airborne and drinking water malodor, but its production and functional ecology are unresolved. this volatile organic compound (voc) is derived from the catalytic breakdown of β-carotene, and evidence indicates that it is produced by the activation of a specific carotene oxygenase by all species of the bloom-forming cyanobacterium microcystis. previous work has shown that β-cyclocitral affects grazer behavior, but th ...201021072572
successfully resisting a pathogen is rarely costly in daphnia magna.a central hypothesis in the evolutionary ecology of parasitism is that trade-offs exist between resistance to parasites and other fitness components such as fecundity, growth, survival, and predator avoidance, or resistance to other parasites. these trade-offs are called costs of resistance. these costs fall into two broad categories: constitutive costs of resistance, which arise from a negative genetic covariance between immunity and other fitness-related traits, and inducible costs of resistan ...201021083915
cloning of the unculturable parasite pasteuria ramosa and its daphnia host reveals extreme genotype-genotype interactions.the degree of specificity in host-parasite interactions has important implications for ecology and evolution. unfortunately, specificity can be difficult to determine when parasites cannot be cultured. in such cases, studies often use isolates of unknown genetic composition, which may lead to an underestimation of specificity. we obtained the first clones of the unculturable bacterium pasteuria ramosa, a parasite of daphnia magna. clonal genotypes of the parasite exhibited much more specific int ...201021091597
transfer modelling and toxicity evaluation of the effluent from an installation of cleansing and uranium recovery using a battery of bioassays.on july 7, 2008, a leak of effluent from an installation of cleansing and uranium recovery (tricastin, france) led to the spillage of uranium in a stream. the acute toxicity of the effluent was evaluated, and compared to the toxicity of uranium nitrate in bioassays using several organisms: chlamydomonas reinhardtii, daphnia magna, chironomus riparius and danio rerio. a sediment bioassay was also performed on c. riparius using water and sediment sampled along the river. results showed that efflue ...201021107686
toxicity evaluation of water samples collected near a hospital waste landfill through bioassays of genotoxicity piscine micronucleus test and comet assay in fish astyanax and ecotoxicity vibrio fischeri and daphnia magna.in this study, we analyzed samples of water from a river and a lake located near a hospital waste landfill with respect to physico-chemical parameters and conducted bioassays of ecotoxicity using vibrio fischeri and daphnia magna, which are species commonly used to evaluate the water toxicity. we also evaluated damage to the genetic material of fish (astyanax sp. b) that were exposed (96 h) to water from these two sites that were located near the tank ditch, using the alkaline comet assay and th ...201021153435
ecotoxicological characterization of tannery wastewater in dhaka, bangladesh.tanning industries are one of the main economic activities in bangladesh. it has been well documented that wastewater discharged from tanneries without appropriate treatment results in detrimental effects on the ecosystem. no ecotoxicity evaluation of any aquatic environment in bangladesh has been conducted so far. in this study a battery of toxicity bioassays and chemical analysis were carried out from water samples obtained from three sampling points: upstream from discharging site on river bu ...201021186722
fusarium oxysporum degradation and detoxification of a new textile-glycoconjugate azo dye (gad).degradation and detoxification of textile dyes are of interest due to the huge environmental impact of such chemicals. an isolate of fusarium oxysporum was used to degrade and to detoxify a new chemical class of textile dyes called glycoconjugate azo dye (gad). after 6 d of growth in a liquid batch culture, the fungus degraded the dye and the culture medium at the end of incubation period showed a ˜100% detoxification compared to the initial dye solution. increasing the initial fungal inoculum, ...201021215952
a comparative toxicity assessment of materials used in aquatic construction.comparative toxicity testing was performed on selected materials that may be used in aquatic construction projects. the tests were conducted on the following materials: (1) untreated wood species (hemlock [tsuga ssp], western red cedar (thuja plicata), red oak [quercus rubra], douglas fir [pseudotsuga menziesii], red pine [pinus resinosa], and tamarack [larix ssp]); (2) plastic wood; (3) ecothermo wood hemlock stakes treated with preservatives (e.g., chromated copper arsenate [cca], creosote, al ...201121222116
effects of pesticides monitored with three sampling methods in 24 sites on macroinvertebrates and microorganisms.grab water samples, sediment samples, and 2,2,4-trimethylpentane passive samplers (trimps) were used to determine the exposure to 97 pesticides in 24 southeast australian stream sites over 5 months. macroinvertebrate communities and selected microorganisms (bacteria, flagellates, ciliates, amoebas, nematodes, and gastrotrichs) were sampled to detect relationships with pesticide toxicity. sediment samples had the highest estimated toxicities in terms of toxic units (tu) for daphnia magna (tu(dm)) ...201121247100
complex evaluation of ecotoxicity and genotoxicity of antimicrobials oxytetracycline and flumequine used in aquaculture.ecotoxicity and genotoxicity of widely used veterinary antimicrobials oxytetracycline and flumequine was studied with six model organisms (vibrio fischeri, pseudomonas putida, pseudokirchneriella subcapitata, lemna minor, daphnia magna, escherichia coli). overall median effective concentration (ec50) values ranged from 0.22 mg/l to 86 mg/l. pseudomonas putida was the most sensitive organism (ec50 values for 16-h growth inhibition were 0.22 and 0.82 mg/l for oxytetracycline and flumequine, respec ...201121312248
resolving the infection process reveals striking differences in the contribution of environment, genetics and phylogeny to host-parasite interactions.infection processes consist of a sequence of steps, each critical for the interaction between host and parasite. studies of host-parasite interactions rarely take into account the fact that different steps might be influenced by different factors and might, therefore, make different contributions to shaping coevolution. we designed a new method using the daphnia magna - pasteuria ramosa system, one of the rare examples where coevolution has been documented, in order to resolve the steps of the i ...201121342515
acute and chronic toxicity of betanal®expert and its active ingredients on nontarget aquatic organisms from different trophic levels.as a way to improve the efficacy to target organisms, new pesticide generation is based on technologically advanced coformulations of two or more active ingredients. one example is betanal®expert, a postemergence herbicide composed of an advanced micro droplet coformulation of phenmedipham, desmedipham, and ethofumesate. although its composed formulation brings an increase in the pesticide performance, it can also enhance its toxicity to nontarget species. therefore, the present study intends to ...201121374783
solid-phase treatment with the fungus trametes versicolor substantially reduces pharmaceutical concentrations and toxicity from sewage sludge.for safe biosolid-land-applying, sludge should be contaminant-free. however, it may contain important amounts of micropollutants, not removed in the wastewater-treatment-processes. an alternative treatment with the fungus trametes versicolor was applied in sterile solid-phase systems consisting of sludge and a lignocellulosic substrate. fungal colonization and activity were demonstrated during the process, according to monitoring of ergosterol, laccase activity and the naproxen-degradation test ...201121376580
evolutionary ecotoxicology of pesticide resistance: a case study in daphnia.natural populations that are exposed to pesticides in their environment may at the same time be exposed to natural stressors like parasites and predators, which may interact with pesticide exposure. this may not only impact target pest species but also a wide variety of non-target species. this review reports on a joint research program in the water flea daphnia magna, a non-target species often used as model organism in ecology and ecotoxicology. the focus is on different aspects that are of ke ...201121380529
food quality of heterotrophic bacteria for daphnia magna: evidence for a limitation by sterols.the quality of heterotrophic bacteria as food for metazoan grazers has been investigated poorly. we conducted growth experiments with juvenile daphnia magna feeding on different strains of heterotrophic bacteria that represent typical pelagic bacteria of five phylogenetically distinct groups. the bacterial food suspensions were supplemented with cholesterol and/or the polyunsaturated fatty acid eicosapentaenoic acid (epa), two essential nutrients that are either absent or scarcely represented in ...201121426363
permanent genetic resources added to molecular ecology resources database 1 october 2010-30 november 2010.this article documents the addition of 277 microsatellite marker loci to the molecular ecology resources database. loci were developed for the following species: ascochyta rabiei, cambarellus chapalanus, chionodraco hamatus, coptis omeiensis, cynoscion nebulosus, daphnia magna, gerbillus nigeriae, isurus oxyrinchus, lates calcarifer, metacarcinus magister, oplegnathus fasciatus, pachycondyla verenae, phaethon lepturus, pimelodus grosskopfii, rotylenchulus reniformis, scomberomorus niphonius, sep ...201121429157
epidemiological, evolutionary, and coevolutionary implications of context-dependent parasitism.abstract victims of infection are expected to suffer increasingly as parasite population growth increases. yet, under some conditions, faster-growing parasites do not appear to cause more damage, and infections can be quite tolerable. we studied these conditions by assessing how the relationship between parasite population growth and host health is sensitive to environmental variation. in experimental infections of the crustacean daphnia magna and its bacterial parasite pasteuria ramosa, we show ...201121460572
assessing lethal and sub-lethal effects of trichlorfon on different trophic levels.trichlorfon (tcf) is one of the most used veterinary pharmaceuticals not only to fight infestations but also as a preventive measure worldwide. the high concentrations used generate concerns about environmental and human health. in this work we assessed the acute toxicity of this compound to non-target organisms belonging to different trophic levels: danio rerio (early life stages and adults), daphnia magna and algae (pseudokirchneriella subcapitata and chlorella vulgaris), and studied the poten ...201121473847
the expression of virulence during double infections by different parasites with conflicting host exploitation and transmission strategies.in many natural populations, hosts are found to be infected by more than one parasite species. when these parasites have different host exploitation strategies and transmission modes, a conflict among them may arise. such a conflict may reduce the success of both parasites, but could work to the benefit of the host. for example, the less-virulent parasite may protect the host against the more-virulent competitor. we examine this conflict using the waterflea daphnia magna and two of its sympatric ...201121481055
chitobiase activity as an indicator of altered survival, growth and reproduction in daphnia pulex and daphnia magna (crustacea: cladocera) exposed to spinosad and diflubenzuron.chitobiase is involved in exoskeleton degradation and recycling during the moulting process in arthropods. in aquatic species, the moulting fluid is released into the aqueous environment, and chitobiase activity present therein can be used to follow the dynamics of arthropod populations. here, chitobiase activity was used for monitoring the impact of mosquito candidate larvicides on daphnia pulex and daphnia magna under laboratory conditions. both species were exposed to spinosad (2, 4, 8μgl(-1) ...201121497397
correlation between physicochemical and ecotoxicological approaches to estimate landfill leachates toxicity.leachates from municipal solid waste (msw) landfills may contain a huge diversity of contaminants; these wastewaters should be considered as potentially hazardous complex mixtures, representing a potential environmental risk for surface and groundwater. current msw landfill wastes regulatory approaches deem exclusively on the physicochemical characterization and does not contemplate the ecotoxicological assessment of landfill leachates. however, the presence of highly toxic substances in consume ...201121530225
application of chemical toxicity distributions to ecotoxicology data requirements under reach.the european union's reach regulation has further highlighted the lack of ecotoxicological data for substances in the marketplace. the mandates under reach (registration, evaluation, authorization, and restriction of chemicals) to produce data and minimize testing on vertebrates present an impetus for advanced hazard assessment techniques using read-across. research in our group has recently focused on probabilistic ecotoxicological hazard assessment approaches using chemical toxicity distributi ...201121590796
differential levels of stress proteins (hsps) in male and female daphnia magna in response to thermal stress: a consequence of sex-related behavioral differences?in two independent experiments, we compared: (1) water depth selection (and accompanying temperature selection) by male and female daphnia magna under different kinds of environmental stress, including the presence of filamentous cyanobacteria, the risk of predation from fish, and the presence of toxic compounds; and (2) sex-dependent production of heat shock proteins (hsp60, 70, and 90) in response to a sudden change in temperature. male d. magna selected deep water strata, which offer a relati ...201121614533
chlorination in a wastewater treatment plant: acute toxicity effects of the effluent and of the recipient water body.this study investigates the impact of wastewater treatment plant (wwtp) effluent on the toxicity of the recipient water body and the effectiveness of the disinfection treatment applied (sodium hypochloride) to assure the compliance of both microbiological and toxicological emission limits. no toxicity was found in the majority of samples collected from the recipient river, upstream and downstream of the wwtp, using three different toxicity tests (vibrio fischeri, daphnia magna, and pseudokirchne ...201121638065
biological surface coating and molting inhibition as mechanisms of tio(2) nanoparticle toxicity in daphnia magna.the production and use of nanoparticles (np) has steadily increased within the last decade; however, knowledge about risks of np to human health and ecosystems is still scarce. common knowledge concerning np effects on freshwater organisms is largely limited to standard short-term (≤48 h) toxicity tests, which lack both np fate characterization and an understanding of the mechanisms underlying toxicity. employing slightly longer exposure times (72 to 96 h), we found that suspensions of nanosized ...201121647422
the potential of cladocerans as controphic competitors of the mosquito culex pipiens.this study assesses the potential of cladocerans as competitors for controlling the oviposition and larval abundances of mosquitoes. control of mosquito larvae involving the use of antagonists has focused mostly on predators. we hypothesized that cladoceran competitors have a strong potential to control larval populations of some species of mosquitoes that can be early colonizers of newly-filled waterbodies, and should be less efficient competitors. to test this hypothesis, the establishment and ...201121661316
composition and stability of the microbial community inside the digestive tract of the aquatic crustacean daphnia magna.small filter-feeding zooplankton organisms like the cladoceran daphnia spp. are key members of freshwater food webs. although several interactions between daphnia and bacteria have been investigated, the importance of the microbial communities inside daphnia guts has been studied only poorly so far. in the present study, we characterised the bacterial community composition inside the digestive tract of a laboratory-reared clonal culture of daphnia magna using 16s rrna gene libraries and terminal ...201121667195
daphnia intoxicated by nerve agent tabun can be treated using human antidotes.application of human antidotes against nerve agent intoxications to microcrustacean daphnia magna (crustacea, cladocera) intoxicated by a nerve agent tabun (o-ethyl-n,n-dimethylphosphoramidocyanidate) and their efficacy was investigated. it was found that antidotes can be successfully applied to intoxicated daphnids. three different treatment regimens were tested: the combination of atropine and acetylcholinesterase reactivator (trimedoxime was chosen), atropine only and trimedoxime alone, too. ...200821783870
genotoxicity and ecotoxicity assays using the freshwater crustacean daphnia magna and the larva of the aquatic midge chironomus riparius to screen the ecological risks of nanoparticle exposure.genotoxic and ecotoxic assessments of widely used nanoparticles, cerium dioxide (ceo(2)), silicon dioxide (sio(2)) and titanium dioxide (tio(2)), were conducted on two aquatic sentinel species, the freshwater crustacean daphnia magna and the larva of the aquatic midge chironomus riparius. ceo(2) may have genotoxic effects on d. magna and c. riparius, given that the dna strand breaks increased in both species when exposed to this nanoparticle; whereas, neither exposure to sio(2) nor tio(2) had a ...200921783986
Effects of a new photoactivatable cationic porphyrin on ciliated protozoa and branchiopod crustaceans, potential components of freshwater ecosystems polluted by pathogenic agents and their vectors.The increasing use of photosensitized processes for disinfection of microbiologically polluted waters requires a precise definition of the factors controlling the degree of photosensitivity in target and non-target organisms. In this regard, tests with protozoa and invertebrates which have a natural habitat in such waters may be used as first screening methods for the assessment of possible hazards for the ecosystem. A new cationic porphyrin, namely meso-tri(N-methyl-pyridyl)mono(N-dodecyl-pyrid ...201122011789
Chronic Effects of Pinus radiata and Eucalyptus globulus Kraft Mill Effluents and Phytosterols on Daphnia magna.Two kraft pulp mill effluents were compared in terms of their chronic toxicity to Daphnia magna. One resulted from pulping Pinus radiata and the other came from a parallel processing of Pinus radiata and Eucalyptus globulus (mixed kraft pulp mill effluent). The concentration of phytosterols found in the mixed kraft pulp mill effluent was higher than in the effluent from Pinus radiata, with values of 0.1082 and 0.02 µg/L, respectively. The phytosterols per se are responsible for 12.9% and 8.1% of ...201121979137
Who benefits from reduced reproduction in parasitized hosts? An experimental test using the Pasteuria ramosa-Daphnia magna system.SUMMARYWe investigated whether parasites or hosts benefit from reduced reproduction in infected hosts. When parasites castrate their hosts, the regain of host reproduction is necessary for castration to be a host adaptation. When infecting Daphnia magna with Pasteuria ramosa, in a lake water based medium, 49·2% of the castrated females regained reproduction. We investigated the relationship between castration level, and parasite and host fitness proxies to determine the adaptive value of host ca ...201121854675
toxicological assessment of tridecafluorohexylethyl methacrylate (6:2 ftmac).the toxicity of tridecafluorohexylethyl methacrylate (6:2 ftmac), an acrylic monomer used in producing polymeric substances, was evaluated. 6:2 ftmac has low acute oral and dermal toxicity (ld50>5000mg/kg), was not a skin or eye irritant, and did not demonstrate skin sensitization potential in a local lymph node assay (llna). 6:2 ftmac was not mutagenic in the bacterial reverse mutation (ames) test or in the mouse lymphoma assay. 6:2 ftmac induced structural aberrations in human peripheral blood ...201122154915
Ecotoxicological assessment of grey water treatment systems with Daphnia magna and Chironomus riparius.In order to meet environmental quality criteria, grey water was treated in four different ways: 1) aerobic 2) anaerobic + aerobic 3) aerobic + activated carbon 4) aerobic + ozone. Since each treatment has its own specific advantages and disadvantages, the aim of this study was to compare the ecotoxicity of differently treated grey water using Chironomus riparius (96 h test) and Daphnia magna (48 h and 21d test) as test organisms. Grey water exhibited acute toxicity to both test organisms. The ae ...201122197265
collateral damage: rapid exposure-induced evolution of pesticide resistance leads to increased susceptibility to parasites.although natural populations may evolve resistance to anthropogenic stressors such as pollutants, this evolved resistance may carry costs. using an experimental evolution approach, we exposed different daphnia magna populations in outdoor containers to the carbamate pesticide carbaryl and control conditions, and assessed the resulting populations for both their resistance to carbaryl as well as their susceptibility to infection by the widespread bacterial microparasite pasteuria ramosa. our resu ...201121884064
automated nanocosm test system to assess the effects of stressors on two interacting populations.there is a great need in environmental research for test systems that include ecologically important factors and that are also easy to use. we present here the automated test system nanocosm, which is composed of populations of daphnia magna and culex pipiens molestus. the nanocosm system allows the investigation of stressed populations in the presence of interspecific competition, which is a very important factor involved in the dynamics of ecosystems. with the nanocosm system, the abundance an ...201122000337
toxicity of electronic waste leachates to daphnia magna: screening and toxicity identification evaluation of different products, components, and materials.electronic waste has become one of the fastest growing waste problems in the world. it contains both toxic metals and toxic organics. the aim of this study was to (1) investigate to what extent toxicants can leach from different electronic products, components, and materials into water and (2) identify which group of toxicants (metals or hydrophobic organics) that is causing toxicity. components from five discarded electronic products (cell phone, computer, phone modem, keyboard, and computer mo ...201122193862
toxicity of individual naphthenic acids to vibrio fischeri.numerous studies have suggested that the toxicity of organic compounds containing at least one carboxylic acid group and broadly classified as "naphthenic acids", is of environmental concern. for example, the acute toxicity of the more than 1 billion m(3) of oil sands process-affected water and the hormonal activity of some offshore produced waters has been attributed to the acids. however, experimental evidence for the toxicity of the individual acids causing these effects has not been very for ...201121942822
Interspecies comparisons on the uptake and toxicity of silver and cerium dioxide nanoparticles.An increasing number and quantity of manufactured nanoparticles are entering the environment as the diversity of their applications increases, and this will lead to the exposure of both humans and wildlife. However, little is known regarding their potential health effects. We compared the potential biological effects of silver (Ag; nominally 35 and 600-1,600?nm) and cerium dioxide (CeO(2;) nominally <25?nm and 1-5?µm) particles in a range of cell (human hepatocyte and intestinal and fish hepato ...201222002553
assessment of the effects of cr, cu, ni and pb soil contamination by ecotoxicological tests.this study aimed to assess soil quality by chemical and ecotoxicological investigations and to check the correspondence between soil metal concentrations and ecotoxicity. for these purposes, surface soils collected at four adjacent roadside urban parks and at a former industrial area were characterized for c/n, organic matter content, texture, and ph. cr, cu, ni and pb, chosen among the most representative soil metal contaminants, were measured as total content and as available and water soluble ...201121918769
activities of jatropha curcas phorbol esters in various bioassays.jatropha curcas seeds contain 30-35% oil, which can be converted to high quality biodiesel. however, jatropha oil is toxic, ascribed to the presence of phorbol esters (pes). in this study, isolated phorbol ester rich fraction (peef) was used to evaluate the activity of pes using three aquatic species based bioassays (snail (physa fontinalis), brine shrimp (artemeia salina), daphnia (daphnia magna)) and microorganisms. in all the bioassays tested, increase in concentration of pes increased mortal ...201122172520
competition increases toxicant sensitivity and delays the recovery of two interacting populations.we investigated how persistent competitive pressure alters toxicant sensitivity and recovery from a pesticide pulse at community level. interacting populations of daphnia (daphnia magna) and culex larvae (culex pipiens molestus) were pulse-exposed (48h) to the pyrethroid fenvalerate. the abundance and biomass of the populations were monitored by non-invasive image analysis. shortly after exposure, daphnia showed a concentration-response relationship with the toxicant with an lc(50) of 0.9μg/l. c ...201222057252
acute and chronic toxicity of benzotriazoles to aquatic organisms.purpose: resulting from their intensive use as corrosion inhibitors in aircraft deicing and anti-icing fluids (adaf) and for silver protection in dishwasher detergents benzotriazoles (bts) are widespread in european surface waters. the current study aimed on an ecotoxicological characterization of 1h-benzotriazole (1h-bt) and 5-methyl-1h-benzotriazole (5mbt). methods: acute and chronic oecd guideline tests were c ...201122203403
daphnia magna, a host for evaluation of bacterial virulence.we show that daphnia magna can be used to assess acute virulence of pathogens relevant to human health, such as pseudomonas aeruginosa or photorhabdus asymbiotica. analysis of bacterial mutants suggests that p. aeruginosa uses similar mechanisms to infect daphnia and other hosts.201222081563
acute toxicity of poly- and perfluorinated compounds to two cladocerans, daphnia magna and chydorus sphaericus.because of their global distribution, environmental persistence and potential risk to human beings and ecosystems, poly- and perfluorinated compounds (pfcs) are of particular concern for research and regulatory communities. however, insufficient toxicity data are available for most poly- and perfluorinated compounds to accurately assess their possible environmental hazard. therefore, the acute toxicity of seven poly- and perfluorinated carboxylic acids and alcohols on two cladocerans, daphnia ma ...201122170568
Acute Toxicity of Alkylpolyglucosides to Vibrio fischeri, Daphnia magna and Microalgae: A Comparative Study.In this paper, toxicity values of alkylpolyglucosides have been determined by applying the 24-h immobilization test with Daphnia magna, the LumiStox(®) 300 test which employs the luminescent bacteria Photobacterium phosphoreum and the test with Selenastrum capricornutum. Three alkylpolyglucosides with different alkyl chain and degree of polymerisation have been tested. For all tests, the results indicated that Vibrio fischeri was more sensitive to toxic effects from alkylpolyglucosides than was ...201122127434
resistance to a bacterial parasite in the crustacean daphnia magna shows mendelian segregation with dominance.the influence of host and parasite genetic background on infection outcome is a topic of great interest because of its pertinence to theoretical issues in evolutionary biology. in the present study, we use a classical genetics approach to examine the mode of inheritance of infection outcome in the crustacean daphnia magna when exposed to the bacterial parasite pasteuria ramosa. in contrast to previous studies in this system, we use a clone of p. ramosa, not field isolates, which allows for a mor ...201122167056
Assessment of Landfill Leachate Toxicity Reduction After Biological Treatment.In the present article, the efficiency of biological treatment of landfill leachates was evaluated by implementation of physicochemical characterisation and a complex toxicity assessment. An array of toxicity tests using bacterium Vibrio fischeri, alga Desmodesmus subspicatus, crustacean Daphnia magna, and embryo of fish Danio rerio, as well as unconventional methods using biochemical biomarkers (protein content, enzymes cholinesterase, and glutathione-S-transferase), were employed. Toxicity of ...201121858736
Acute and chronic toxicity of four frequently used UV filter substances for Desmodesmus subspicatus and Daphnia magna.As a consequence of growing public concern about UV radiation effects on human health chemical and physical UV filters are increasingly used in personal care and other products. The release of these lipophilic and often persistent compounds into surface waters may pose a risk for aquatic organisms. The aim of the study was to determine effects of four frequently used UV filters on primary aquatic producers and consumers, the green alga Desmodesmus subspicatus and the crustacean Daphnia magna. Ex ...201121929467
genetic variation for maternal effects on parasite susceptibility.the expression of infectious disease is increasingly recognized to be impacted by maternal effects, where the environmental conditions experienced by mothers alter resistance to infection in offspring, independent of heritability. here, we studied how maternal effects (high or low food availability to mothers) mediated the resistance of the crustacean daphnia magna to its bacterial parasite pasteuria ramosa. we sought to disentangle maternal effects from the effects of host genetic background by ...201121848987
evaluation of the hazardous impact of landfill leachates by toxicity and biodegradability tests.the aim of our research was to assess the ecotoxicity and biodegradability of leachates originating from two parts of a municipal landfill before and after biological treatment in the existing treatment plant. biotests represent important tools for adequate environmental characterization of landfill leachates and could be helpful in reliable assessment and monitoring of the treatment plant efficiency. for ecotoxicity testing of landfill leachate before and after biological treatment, different o ...201121970176
a contribution towards the risk assessment of soils from the são domingos mine (portugal): chemical, microbial and ecotoxicological indicators.this study is a contribution towards a risk assessment of the são domingos mine area (portugal), integrating information from: soil physicochemical characteristics, pseudo-total and bioavailable trace elements (as, cd, cr, cu, ni, pb and zn), ecotoxicological evaluation, and microbial indicators. the bioassays using soil eluates (seed germination, luminescent inhibition of vibrio fischeri and daphnia magna immobilization) confirmed the soil toxicity categorization obtained with the bioassays usi ...201222230067
aquatic ecotoxicity assessment of a new natural formicide.background, aim and scope: agrochemicals could reach aquatic ecosystems and damage ecosystem functionality. natural formicide could be an alternative to use in comparison with the more toxic formicides available on the market. thus, the objective of this study was to assess the ecotoxicity of the new natural formicide macex® with a battery of classical aquatic ecotoxicity tests. material and methods: bacteria (al ...201222252383
the bacterial parasite pasteuria ramosa is not killed if it fails to infect: implications for coevolution.strong selection on parasites, as well as on hosts, is crucial for fueling coevolutionary dynamics. selection will be especially strong if parasites that encounter resistant hosts are destroyed and diluted from the local environment. we tested whether spores of the bacterial parasite pasteuria ramosa were passed through the gut (the route of infection) of their host, daphnia magna, and whether passaged spores remained viable for a "second chance" at infecting a new host. in particular, we tested ...201223467806
a novel approach to parasite population genetics: experimental infection reveals geographic differentiation, recombination and host-mediated population structure in pasteuria ramosa, a bacterial parasite of daphnia.the population structure of parasites is central to the ecology and evolution of host-parasite systems. here, we investigate the population genetics of pasteuria ramosa, a bacterial parasite of daphnia. we used natural p. ramosa spore banks from the sediments of two geographically well-separated ponds to experimentally infect a panel of daphnia magna host clones whose resistance phenotypes were previously known. in this way, we were able to assess the population structure of p. ramosa based on g ...201223279064
local genetic adaptation generates latitude-specific effects of warming on predator-prey interactions.temperature effects on predator-prey interactions are fundamental to better understand the effects of global warming. previous studies never considered local adaptation of both predators and prey at different latitudes, and ignored the novel population combinations of the same predator-prey species system that may arise because of northward dispersal. we set up a common garden warming experiment to study predator-prey interactions between ischnura elegans damselfly predators and daphnia magna zo ...201223504827
extinction hazards in experimental daphnia magna populations: effects of genotype diversity and environmental variation.extinction is ubiquitous in natural systems and the ultimate fate of all biological populations. however, the factors that contribute to population extinction are still poorly understood, particularly genetic diversity and composition. a laboratory experiment was conducted to examine the influences of environmental variation and genotype diversity on persistence in experimental daphnia magna populations. populations were initiated in two blocks with one, two, three, or six randomly selected and ...201223467276
comparison of the biological effects of humic materials under acidified conditions.the acute and chronic effects of three types of humic material were studied using daphnia magna at ph 5, 6, and 7. at neutral ph humic material had no biological effect. at ph 6 and 5 maximum survival and population growth rate occurred at intermediate levels of humus material (10 mg c/l, 300 mg pt/l). lowest survival and population growth rate occurred at ph 5 in either the absence of humus material or at concentrations exceeding 10 mg c/l (300 mg pt/l). a second order polynomial was used to fi ...20102953070
protein and nucleic acid content in daphnia magna during chronic exposure to cadmium.daphnia magna straus were exposed to cadmium concentrations of 0, 0.4, 0.8, 2.1, 4.3, and 7.2 micrograms/liter for 21 days. protein, rna, dna, glycogen and lipid content were measured after 4, 7, and 21 days of exposure, and the results were related to survival and reproduction. the no observed effect concentration (noec) for survival, protein growth, and reproduction at day 21 was 2.1, 0.8, and 0.8 microgram/liter cadmium, respectively. protein growth was most sensitive to cadmium exposure foll ...20072440658
effects of fenvalerate on biochemical parameters, survival, and reproduction of daphnia magna.daphnia magna were exposed to fenvalerate at nominal concentrations of 0.5, 0.25, 0.13, 0.06, and 0.03 micrograms/liter for 21 days. on days 7 and 21 of exposure, levels of ribonucleic acid (rna), deoxyribonucleic acid (dna), adenosine diphosphate (adp), adenosine triphosphate (atp), glycogen, and lipid were measured, and the results were related to survival and reproduction during the 21-day test period. survival was not significantly (alpha = 0.05) affected by the 21-day exposure; however, rep ...20133757868
approaches to modeling toxic responses of aquatic organisms to aromatic hydrocarbons.acute toxic effects on salmo gairdneri, poecilia reticulata, daphnia magna, and the growth inhibition of selenastrum capricornutum were measured for eight aromatic hydrocarbons. toxicity measurements were carried out following the oecd standard guidelines modified as appropriate in order to prevent hydrocarbon loss by volatilization; the concentrations of the substrates in the test solutions were periodically measured by hplc. the toxic responses were analyzed in terms of the n-octanol/water par ...20113234290
chronic toxicity of aniline and 2,4-dichlorophenol to daphnia magna straus. 20123345354
directional differences in the colour sensitivity of daphnia magna. 20154412836
enzyme variability in natural populations of daphnia magna. ii. genotypic frequencies in permanent populations.in permanent habitats populations of the cyclical parthenogen, daphnia magna, reproduce by continued parthenogenesis and are subject to only sporadic sexual recruitment. the genetic effects of this breeding system have been investigated by analyzing allozyme frequencies in thirteen populations of d. magna.-genotypic frequencies at polymorphic loci characteristically deviated markedly from hardy-weinberg proportions and gametic phase imbalance between loci was frequent. genotypic frequencies were ...20164847154
acute toxicity of cresols, xylenols, and trimethylphenols to daphnia magna straus 1820.twenty-four-hour ic50 values (50% immobilization concentration) for phenol, o-cresol, m-cresol, p-cresol, six xylenols, and three trimethylphenols were determined for daphnia magna under static conditions. our results show that cresols are more toxic than phenol, that xylenols do not exhibit significantly higher toxicity than cresols, and that trimethylphenols are less toxic than cresols. thus, no direct relationship can be found between the number and position of methyl groups on the phenol nuc ...20113238416
acute toxicity and qsar of chlorophenols on daphnia magna. 20093768559
the role of moulting in parasite defence.parasitic infections consist of a succession of steps during which hosts and parasites interact in specific manners. at each step, hosts can use diverse defence mechanisms to counteract the parasite's attempts to invade and exploit them. of these steps, the penetration of parasites into the host is a key step for a successful infection and the epithelium is the first line of host defence. the shedding of this protective layer (moulting) is a crucial feature in the life cycle of several invertebr ...201222496187
accumulation and inactivation of avian influenza virus by the filter-feeding invertebrate daphnia magna.the principal mode of avian influenza a virus (aiv) transmission among wild birds is thought to occur via an indirect fecal-oral route, whereby individuals are exposed to virus from the environment through contact with virus-contaminated water. aiv can remain viable for an extended time in water; however, little is known regarding the influence of the biotic community (i.e., aquatic invertebrates) on virus persistence and infectivity in aquatic environments. we conducted laboratory experiments t ...201324038705
benefits of host genetic diversity for resistance to infection depend on parasite diversity.host populations with high genetic diversity are predicted to have lower levels of infection prevalence. this theory assumes that host genetic diversity results in variation in susceptibility and that parasites exhibit variation in infectivity. empirical studies on the effects of host heterogeneity typically neglect the role of parasite diversity. we conducted three laboratory experiments designed to test if genetic variation in daphnia magna populations and genetic variation in its parasites to ...201020503859
application of a battery of biotests for the determination of leachate toxicity to bacteria and invertebrates from sewage sludge-amended soil.the objective of the study was to determine the leachates toxicity from sewage sludge-amended soils (sandy and loamy). samples originated from a plot experiment realized over a period of 29 months. two types of soil were fertilized with sewage sludges at the dose of 3 % (90 t/ha). soil samples were taken after 0, 7, 17, and 29 months from the application of sewage sludges. leachates were obtained according to the en 12457-2 protocol. the following commercial tests were applied for the estimation ...201223132406
biological removal of azo and triphenylmethane dyes and toxicity of process by-products.increasing environmental pollution is connected with broad applications of dyes and imperfection of dyeing technology. decolourization of triphenylmethane brilliant green and disazo evans blue by bacterial and fungal strains and toxicity (phyto- and zootoxicity) of degradation by-products were investigated. influence of incubation method on dyes removal was evaluated (static, semi-static, shaken). dead biomass was used for sorption estimation. toxicity of treated dyes was measured to estimate po ...201122593606
biotests and biosensors for ecotoxicology of metal oxide nanoparticles: a minireview.nanotechnologies have become a significant priority worldwide. several manufactured nanoparticles - particles with one dimension less than 100 nm - are increasingly used in consumer products. at nanosize range, the properties of materials differ substantially from bulk materials of the same composition, mostly due to the increased specific surface area and reactivity, which may lead to increased bioavailability and toxicity. thus, for the assessment of sustainability of nanotechnologies, hazards ...200827873807
evaluation of zosteric acid for mitigating biofilm formation of pseudomonas putida isolated from a membrane bioreactor system.this study provides data to define an efficient biocide-free strategy based on zosteric acid to counteract biofilm formation on the membranes of submerged bioreactor system plants. 16s rrna gene phylogenetic analysis showed that gammaproteobacteria was the prevalent taxa on fouled membranes of an italian wastewater plant. pseudomonas was the prevalent genus among the cultivable membrane-fouler bacteria and pseudomonas putida was selected as the target microorganism to test the efficacy of the an ...201424879523
biodegradation and detoxification of textile azo dyes by bacterial consortium under sequential microaerophilic/aerobic processes.release of textile azo dyes to the environment is an issue of health concern while the use of microorganisms has proved to be the best option for remediation. thus, in the present study, a bacterial consortium consisting of providencia rettgeri strain hsl1 and pseudomonas sp. suk1 has been investigated for degradation and detoxification of structurally different azo dyes. the consortium showed 98-99 % decolorization of all the selected azo dyes viz. reactive black 5 (rb 5), reactive orange 16 (r ...201526417357
genome sequence of shinella sp. strain dd12, isolated from homogenized guts of starved daphnia magna.shinella sp. strain dd12, a novel phosphite assimilating bacterium, has been isolated from homogenized guts of 4 days starved zooplankton daphnia magna. here we report the draft genome of this bacterium, which comprises 7,677,812 bp and 7505 predicted protein-coding genes.201626865909
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