| phylogenetic analysis of cronobacter isolates based on the rpoa and 16s rrna genes. | the reclassification of the genus cronobacter (previously known as enterobacter sakazakii) was based on a polyphasic analysis that led to the description of five species. these bacteria are opportunistic pathogens that can cause neonatal meningitis and other infections in immuno-compromised individuals. cronobacter species have been reported to show differences in sensitivity to antibiotics, heat and chemicals, as well as differences in virulence. the objective of this st ... | 2012 | 22237982 |
| mitogenic response and probiotic characteristics of lactic acid bacteria isolated from indigenously pickled vegetables and fermented beverages. | lactic acid bacteria from indigenous pickled vegetables and fermented beverages (fermented rice and madhuca longifolia flowers) were isolated and investigated for their functional characteristics in vitro as potential new probiotic strains. four isolates (all lactobacillus spp.) selected on the basis of high tolerance to bile (0.2%) were identified by standard and molecular methods (16s rdna) as l. helveticus, l. casei, l. delbrueckii and l. bulgaricus from pickled vegetables and fermented bever ... | 2011 | 22806866 |
| cronobacter condimenti sp. nov., isolated from spiced meat, and cronobacter universalis sp. nov., a species designation for cronobacter sp. genomospecies 1, recovered from a leg infection, water and food ingredients. | a re-evaluation of the taxonomic position of five strains, one assigned to cronobacter sakazakii (strain 1330(t), isolated from spiced meat purchased in slovakia), two previously assigned to cronobacter genomospecies 1 (strains nctc 9529(t) and 731, isolated from water and a leg infection, respectively) and two previously assigned to cronobacter turicensis (strains 96 and 1435, isolated from onion powder and rye flour, respectively) was carried out. the analysis included phenotypic characterizat ... | 2011 | 22661070 |
| incidence of bacteria of public health interest carried by cockroaches in different food-related environments. | the aim of this study was to determine the incidence of bacteria of public health interest transmitted by cockroaches in different food-related environments. from april to november, cockroaches were trapped in 11 buildings in different urban areas of western andalusia (spain): three hotels, four grocery stores, a catering establishment, a food-industry plant, a health center, and a care home. the presence of a number of bacterial species, including salmonella, in these food-related environments ... | 2012 | 23270179 |
| biofilm formation and exopolysaccharide (eps) production by cronobacter sakazakii depending on environmental conditions. | biofilm matrices are formed largely of extracellular polymeric substance (eps). this study was conducted to investigate biofilm formation and eps production by cronobacter sakazakii under various conditions (media, nutrition, and relative humidity (rh)) by quantification of eps and cell populations, field emission scanning electron microscope (fe-sem), and colony observation. various agar media conditions (tsa without dextrose (w/d), m9 minimum salt medium (msm) agar, and m9 msm agar with 3% glu ... | 2012 | 23498180 |
| cronobacter sakazakii dna detection in cerebrospinal fluid of a patient with amyotrophic lateral sclerosis mimic syndrome. | a 45-year-old male noticed progressive weakness of the right lower limb with gait disturbance. over the following months, motor deficits worsened, spreading to the right upper limb. electromyography showed active denervation in the upper and lower limb muscles. a diagnosis of amyotrophic lateral sclerosis (als) was made. about 2 years after symptom onset, gradual improvement occurred. cerebrospinal fluid analysis performed about 3 years after the beginning of symptoms identified cronobacter saka ... | 2015 | 26955334 |
| plasmid-encoded mcp is involved in virulence, motility, and biofilm formation of cronobacter sakazakii atcc 29544. | the aim of this study was to elucidate the function of the plasmid-borne mcp (methyl-accepting chemotaxis protein) gene, which plays pleiotropic roles in cronobacter sakazakii atcc 29544. by searching for virulence factors using a random transposon insertion mutant library, we identified and sequenced a new plasmid, pcsa2, in c. sakazakii atcc 29544. an in silico analysis of pcsa2 revealed that it included six putative open reading frames, and one of them was mcp. the mcp mutant was defective fo ... | 2014 | 25332122 |
| screening of genes involved in interactions with intestinal epithelial cells in cronobacter sakazakii. | cronobacter sakazakii possesses a significant ability to adhere to and invade epithelial cells in its host. however, the molecular mechanisms underlying this process are poorly understood. in the current study, the adhesive and invasive capabilities of 56 c. sakazakii strains against human epithelial cells were evaluated, and one of them was selected for construction of a mutant library using the tn5 transposon. in a systematic analysis of the adhesive and invasive capabilities of 1084 mutants, ... | 2016 | 27637944 |
| human isolates of cronobacter sakazakii bind efficiently to intestinal epithelial cells in vitro to induce monolayer permeability and apoptosis. | cronobacter sakazakii (cs) is an emerging opportunistic pathogen that causes life-threatening infections in infants. this pathogen has been implicated in the outbreaks of necrotizing enterocolitis (nec) with associated rates of high mortality and morbidity. in this study, we compared the abilities of cs strains isolated from human and environmental sources to bind to intestinal epithelial cells and trigger apoptosis. | 2011 | 22221600 |
| multiplex detection of nine food-borne pathogens by mpcr and capillary electrophoresis after using a universal pre-enrichment medium. | routine microbiological quality analyses in food samples require, in some cases, an initial incubation in pre-enrichment medium. this is necessary in order to ensure that small amounts of pathogenic strains are going to be detected. in this work, a universal pre-enrichment medium has been developed for the simultaneous growth of bacillus cereus, campylobacter jejuni, clostridium perfringens, cronobacter sakazakii, escherichia coli, enterobacteriaceae family (38 species, 27 genera), listeria mono ... | 2015 | 26579100 |
| rapid detection and simultaneous genotyping of cronobacter spp. (formerly enterobacter sakazakii) in powdered infant formula using real-time pcr and high resolution melting (hrm) analysis. | cronobacter spp. is an emerging pathogen that causes meningitis, sepsis, bacteremia, and necrotizing enterocolitis in neonates and children. the present study developed an assay integrating real-time pcr and high resolution melting (hrm) analysis targeting the ompa gene for the specific detection and rapid identification of cronobacter spp. (formerly enterobacter sakazakii) in powdered infant formula. eleven cronobacter field isolates and 25 reference strains were examined using one pair of prim ... | 2013 | 23825624 |
| cronobacter sakazakii: stress survival and virulence potential in an opportunistic foodborne pathogen. | a characteristic feature of the opportunistic foodborne pathogen cronobacter sakazakii is its ability to survive in extremely arid environments, such as powdered infant formula, making it a dangerous opportunistic pathogen of individuals of all age groups, especially infants and neonates. herein, we provide a brief overview of the pathogen; clinical manifestations, environmental reservoirs and our current understanding of stress response mechanisms and virulence factors which allow it to cause d ... | 2014 | 25562731 |
| investigating the responses of cronobacter sakazakii to garlic-drived organosulfur compounds: a systematic study of pathogenic-bacterium injury by use of high-throughput whole-transcriptome sequencing and confocal micro-raman spectroscopy. | we present the results of a study using high-throughput whole-transcriptome sequencing (rna-seq) and vibrational spectroscopy to characterize and fingerprint pathogenic-bacterium injury under conditions of unfavorable stress. two garlic-derived organosulfur compounds were found to be highly effective antimicrobial compounds against cronobacter sakazakii, a leading pathogen associated with invasive infection of infants and causing meningitis, necrotizing entercolitis, and bacteremia. rna-seq show ... | 2014 | 24271174 |
| my 40-year history with cronobacter/enterobacter sakazakii - lessons learned, myths debunked, and recommendations. | much has been learned about organism in the cronobacter/enterobacter sakazakii complex since i first named and described enterobacter sakazakii in 1980. however, there are still wide knowledge gaps. one of the most serious is that are still many uncertainties associated with assessing the public health risk posed by these bacteria, particularly in neonatal meningitis. over the last few decades, cronobacter contamination of commercial powdered infant formula products has apparently been reduced, ... | 2015 | 26640778 |
| comparative resistance of bacterial foodborne pathogens to non-thermal technologies for food preservation. | in this paper the resistance of bacterial foodborne pathogens to manosonication (ms), pulsed electric fields (pefs), high hydrostatic pressure (hhp), and uv-light (uv) is reviewed and compared. the influence of different factors on the resistance of bacterial foodborne pathogens to these technologies is also compared and discussed. only results obtained under harmonized experimental conditions have been considered. this has allowed us to establish meaningful comparisons and draw significant conc ... | 2016 | 27242749 |
| biocontrol and rapid detection of food-borne pathogens using bacteriophages and endolysins. | bacteriophages have been suggested as natural food preservatives as well as rapid detection materials for food-borne pathogens in various foods. since listeria monocytogenes-targeting phage cocktail (listshield) was approved for applications in foods, numerous phages have been screened and experimentally characterized for phage applications in foods. a single phage and phage cocktail treatments to various foods contaminated with food-borne pathogens including e. coli o157:h7, salmonella enterica ... | 2016 | 27092128 |
| development of a custom-designed, pan genomic dna microarray to characterize strain-level diversity among cronobacter spp. | cronobacter species cause infections in all age groups; however neonates are at highest risk and remain the most susceptible age group for life-threatening invasive disease. the genus contains seven species:cronobacter sakazakii, cronobacter malonaticus, cronobacter turicensis, cronobacter muytjensii, cronobacter dublinensis, cronobacter universalis, and cronobacter condimenti. despite an abundance of published genomes of these species, genomics-based epidemiology of the genus is not well establ ... | 2015 | 25984509 |
| insights into the emergent bacterial pathogen cronobacter spp., generated by multilocus sequence typing and analysis. | cronobacter spp. (previously known as enterobacter sakazakii) is a bacterial pathogen affecting all age groups, with particularly severe clinical complications in neonates and infants. one recognized route of infection being the consumption of contaminated infant formula. as a recently recognized bacterial pathogen of considerable importance and regulatory control, appropriate detection, and identification schemes are required. the application of multilocus sequence typing (mlst) and analysis (m ... | 2012 | 23189075 |
| sialic acid utilization by cronobacter sakazakii. | the cronobacter genus is composed of seven species, and can cause infections in all age groups. of particular concern is c. sakazakii, as this species is strongly associated with severe and often fatal cases of necrotizing enterocolitis and meningitis in neonates and infants. whole genome sequencing has revealed that the nanakt gene cluster required for the utilisation of exogenous sialic acid is unique to the c. sakazakii species (esa_03609-13).sialic acid is found in breast milk, infant formul ... | 2013 | 23706082 |
| cronobacter, the emergent bacterial pathogen enterobacter sakazakii comes of age; mlst and whole genome sequence analysis. | following the association of cronobacter spp. to several publicized fatal outbreaks in neonatal intensive care units of meningitis and necrotising enterocolitis, the world health organization (who) in 2004 requested the establishment of a molecular typing scheme to enable the international control of the organism. this paper presents the application of next generation sequencing (ngs) to cronobacter which has led to the establishment of the cronobacter pubmlst genome and sequence definition data ... | 2014 | 25515150 |
| diversity of the cronobacter genus as revealed by multilocus sequence typing. | cronobacter (previously known as enterobacter sakazakii) is a diverse bacterial genus consisting of seven species: c. sakazakii, c. malonaticus, c. turicensis, c. universalis, c. muytjensii, c. dublinensis, and c. condimenti. in this study, we have used a multilocus sequence typing (mlst) approach employing the alleles of 7 genes (atpd, fusa, glns, gltb, gyrb, infb, and ppsa; total length, 3,036 bp) to investigate the phylogenetic relationship of 325 cronobacter species isolates. strains were ch ... | 2012 | 22785185 |
| identification and characterization of cronobacter iron acquisition systems. | cronobacter spp. are emerging pathogens that cause severe infantile meningitis, septicemia, or necrotizing enterocolitis. contaminated powdered infant formula has been implicated as the source of cronobacter spp. in most cases, but questions still remain regarding the natural habitat and virulence potential for each strain. the iron acquisition systems in 231 cronobacter strains isolated from different sources were identified and characterized. all cronobacter spp. have both the feo and efe syst ... | 2012 | 22706064 |
| capsular profiling of the cronobacter genus and the association of specific cronobacter sakazakii and c. malonaticus capsule types with neonatal meningitis and necrotizing enterocolitis. | cronobacter sakazakii and c. malonaticus can cause serious diseases especially in infants where they are associated with rare but fatal neonatal infections such as meningitis and necrotising enterocolitis. | 2015 | 26449318 |
| genetic analysis of the cronobacter sakazakii o4 to o7 o-antigen gene clusters and development of a pcr assay for identification of all c. sakazakii o serotypes. | the gram-negative bacterium cronobacter sakazakii is an emerging food-borne pathogen that causes severe invasive infections in neonates. variation in the o-antigen lipopolysaccharide in the outer membrane provides the basis for gram-negative bacteria serotyping. the o-antigen serotyping scheme for c. sakazakii, which includes seven serotypes (o1 to o7), has been recently established, and the o-antigen gene clusters and specific primers for three c. sakazakii serotypes (o1, o2, and o3) have been ... | 2012 | 22447597 |
| isolation of cronobacter spp. (enterobacter sakazakii) from artisanal mozzarella. | cronobacter spp. (enterobacter sakazakii) is an opportunistic bacterial pathogen capable of causing disease and even fatalities in newborn infants within the first weeks of life if consumed as part of the diet. premature and immunocompromised newborn infants are at particular risk. the microorganism has been isolated from a variety of foods including contaminated infant milk formula powder and milk powder substitute. the study aimed to evaluate the level of microbiological contamination in 47 sa ... | 2014 | 27800308 |
| characterization of inci1 sequence type 71 epidemic plasmid lineage responsible for the recent dissemination of ctx-m-65 extended-spectrum β-lactamase in the bolivian chaco region. | during the last decade, a significant diffusion of ctx-m-type extended-spectrum β-lactamases (esbls) was observed in commensal escherichia coli from healthy children in the bolivian chaco region, with initial dissemination of ctx-m-2, which was then replaced by ctx-m-15 and ctx-m-65. in this work, we demonstrate that the widespread dissemination of ctx-m-65 observed in this context was related to the polyclonal spreading of an inci1 sequence type 71 (st71) epidemic plasmid lineage. the structure ... | 2015 | 26100713 |
| ingested salmonella enterica, cronobacter sakazakii, escherichia coli o157:h7, and listeria monocytogenes: transmission dynamics from adult house flies to their eggs and first filial (f1) generation adults. | the mechanical transmission of pathogenic bacteria by synanthropic filth flies is widely recognized. while many studies report the fate and the temporospatial distribution of ingested foodborne bacteria by filth flies, there is little evidence about the transmission dynamics of ingested foodborne bacteria by adult house flies (musca domestica) to their progeny. in this study, we fed parental house fly adults with food contaminated with low, medium, and high concentrations of salmonella enterica, ... | 2015 | 26228457 |
| comparative genotypic and phenotypic analysis of cronobacter species cultured from four powdered infant formula production facilities: indication of pathoadaptation along the food chain. | cronobacter species are opportunistic pathogens commonly found in the environment. among the seven cronobacter species, cronobacter sakazakii sequence type 4 (st-4) is predominantly associated with recorded cases of infantile meningitis. this study reports on a 26-month powdered infant formula (pif) surveillance program in four production facilities located in distinct geographic regions. the objective was to identify the st(s) in pif production environments and to investigate the phenotypic fea ... | 2015 | 25911470 |
| detection of cronobacter sakazakii in powdered infant formula using an immunoliposome-based immunomagnetic concentration and separation assay. | this study aimed to optimize the applicability of an immunoliposome-based immunomagnetic concentration and separation assay to facilitate rapid detection of cronobacter sakazakii in powdered infant formula (pif). to determine the detection limit, specificity, and pre-enrichment incubation time (0, 4, 6, and 8 h), assay tests were performed with different cell numbers of c. sakazakii (2 × 10(0) and 2 × 10(1) cfu/ml) inoculated in 10 g of pif. the assay was able to detect as few as 2 cells of c. s ... | 2016 | 27721500 |
| time for the 70°c water precautionary option in the home dilution of powdered infant formula. | powdered infant formulas (pif) are usually not sterile and may frequently be contaminated by several bacteria strains. among them, cronobacter species, previously known as enterobacter sakazakii, is one of the most harmful, since it might be the causative agent of sepsis and meningitis in newborns and preterm infants during the first weeks of life. the mortality rate of these infections is up to 80 %. therefore, some precautions are required in the home handling and dilution of pif. whereas ther ... | 2016 | 26895705 |
| prevalence and antimicrobial resistance pattern of bacterial strains isolated from patients with urinary tract infection in messalata central hospital, libya. | to investigate the prevalence of urinary tract infection among patients at messalata central hospital, libya, to identify the causative bacteria, and to explore their resistance pattern to antimicrobials. | 2016 | 27569886 |
| isolation and antimicrobial testing of aeromonas spp., citrobacter spp., cronobacter spp., enterobacter spp., escherichia spp., klebsiella spp., and trabulsiella spp. from the gallbladder of pigs. | the presence of gram-negative bacteria species, other than salmonella spp., in the gallbladder of pigs was examined. isolated gram-negative bacteria were assigned to species using the microgen™ gna+b-id systems. of the 64 isolated strains 43 were identified as escherichia coli, seven as enterobacter spp., three each as klebsiella spp., citrobacterfreundii, aeromonas hydrophila and cronobacter sakazakii and one each as escherichiafergusonii and trabulsiella guamensis. their antibiograms showed ve ... | 2015 | 26373181 |
| inhibition of quorum-sensing-mediated biofilm formation in cronobacter sakazakii strains. | the present study investigated plant extracts for their anti-quorum-sensing (qs) potential to inhibit the biofilm formation in cronobacter sakazakii strains. the bioassay based on loss of pigment production by chromobacterium violaceum 026 and agrobacterium tumefaciens ntl4(pzlr4) was used for initial screening of the extracts. further, the effect of extracts on the inhibition of qs-mediated biofilm in c. sakazakii isolates was evaluated using standard crystal violet assay. the effect on biofilm ... | 2016 | 27460095 |
| [detection of biofilm formation by selected pathogens relevant to the food industry]. | detection of biofilm formation by microbial pathogens relevant to the food industry and comparison of biofilm formation under different conditions of culture. | 2015 | 26448305 |
| analysis of microflora profile in korean traditional nuruk. | a variety of nuruk were collected from various provinces in korea, and their microflora profiles were analyzed at the species level. a total of 42 nuruk samples were collected and when the viable cell numbers in these nuruk were enumerated, the average cell numbers of bacteria, fungi, yeast, and lactic acid bacteria from all nuruk were 7.21, 7.91, 3.49, and 4.88 log cfu/10 g, respectively. there were no significant differences in viable cell numbers of bacteria or fungi according to regions coll ... | 2013 | 23314366 |
| antimicrobial activity of kefir against various food pathogens and spoilage bacteria. | kefir is a unique fermented dairy product produced by a mixture of lactic acid bacteria, acetic acid bacteria, and yeast. here, we compared the antimicrobial spectra of four types of kefirs (a, l, m, and s) fermented for 24, 36, 48, or 72 h against eight food-borne pathogens. bacillus cereus, staphylococcus aureus, listeria monocytogenes, enterococcus faecalis, escherichia coli, salmonella enteritidis, pseudomonas aeruginosa, and cronobacter sakazakii were used as test strains, and antibacterial ... | 2016 | 28115890 |
| simultaneous identification of 13 foodborne pathogens by using capillary electrophoresis-single strand conformation polymorphism coupled with multiplex ligation-dependent probe amplification and its application in foods. | capillary electrophoresis-single strand conformation polymorphism (ce-sscp) coupled with stuffer-free multiplex ligation-dependent probe amplification (mlpa) was developed to identify 13 species of foodborne pathogens simultaneously. species-specific mlpa probes were designed for nine of these species. these probes were targeted to the groel, glya, mms, tuf, inv, ipah, nuc, vvh, and 16s rrna genes, which corresponded to bacillus cereus, campylobacter coli, cronobacter sakazakii, enterococcus spp ... | 2016 | 27494415 |
| hygienic characteristics and microbiological hazard identification in horse and donkey raw milk. | today the interest toward horse (equus caballus) and donkey (equus asinus) milk for human consumption is receiving a renewed attention because of its particular composition, hypoallergenicity, and nutraceutical properties. the realistic perspective of global use of this aliment in balanced diets, especially for infancy and geriatrics, poses the need for a more in depth knowledge on milk hygiene and on the health status of dairy animals, as a prerequisite of consumers' safety. the aim of this pap ... | 2017 | 27033528 |
| evaluation of probiotic properties of lactobacillus plantarum wlpl04 isolated from human breast milk. | lactobacillus plantarum wlpl04, a specific strain isolated from human breast milk, was investigated for its survival capacity (acid and bile salt tolerance, survival in simulated gastrointestinal tract, inhibition of pathogens, antibiotic susceptibility, yield of exopolysaccharides) and probiotic properties (antiadhesion of pathogens, protection from harmful effect of sodium dodecyl sulfate, and antiinflammatory stress on caco-2 cells). the results showed that lb. plantarum wlpl04 had broad-spec ... | 2016 | 26805974 |
| rapid detection of cronobacter sakazakii by real-time pcr based on the cgca gene and taqman probe with internal amplification control. | cronobacter sakazakii is a severe virulent strain that is frequently detected in powdered infant formula (pif). therefore, it is necessary to develop a fast and specific detection method. the specificity of our newly developed quantitative real-time pcr (qrt-pcr) was validated with dna from 46 strains. among them, 12 c. sakazakii strains were correctly amplified, whereas no positive florescent signal was observed from 34 nontarget controls. the detection limit of c. sakazakii was about 110 cfu/m ... | 2016 | 26751178 |
| antioxidant and antibacterial activities of exopolysaccharides from bifidobacterium bifidum wbin03 and lactobacillus plantarum r315. | the objective of this study was to investigate the antioxidant and antibacterial activities of exopolysaccharide (eps) from bifidobacterium bifidum wbin03 (b-eps) and lactobacillus plantarum r315 (l-eps). the 1,1-diphenyl-2-picrylhydrazyl (dpph)-radical scavenging, hydroxyl radical-scavenging, and superoxide radical-scavenging abilities were measured to evaluate antioxidant activity. inhibition of erythrocyte hemolysis and lipid peroxidation was also measured. both b-eps and l-eps had strong sca ... | 2014 | 25282420 |
| development of 23 individual taqman® real-time pcr assays for identifying common foodborne pathogens using a single set of amplification conditions. | most of the acute intestinal diseases are caused by foodborne pathogens with infants and elderly people being at major risk. the aim of this study was to develop a procedure to simultaneously detect 20 foodborne pathogens in complex alimentary matrices such as milk, cheese and meat. the list of targets include, among the others, listeria spp., salmonella spp., shigella spp., escherichia coli spp., campylobacter spp., clostridium spp. and staphylococcus aureus. the accuracy of detection was deter ... | 2014 | 24929880 |
| antibacterial effectiveness of chitosan-propolis coated polypropylene films against foodborne pathogens. | antibacterial properties of chitosan are well documented in the literature. however its antibacterial effectiveness in the film form is controversial due to the methodological differences in test methods used. in this study, antibacterial effectiveness of chitosan-coated polypropylene films alone and incorporating ethanolic extract of propolis (eep) were evaluated against six foodborne pathogens (bacillus cereus, cronobacter sakazakii, escherichia coli o157:h7, listeria monocytogenes, salmonella ... | 2013 | 23707735 |
| phytochemical profiles of volatile constituents from centaurea ragusina leaves and flowers and their antimicrobial effects. | the volatile oils obtained from the leaves and flowers of the croatian endemic plant centaurea ragusina l. were evaluated for antimicrobial activity. the chemical compositions, determined by gc and gc-ms, were complex, with sesquiterpenes the most abundant class of compound. nonterpene hydrocarbons were also identified in high quantities, while acids and ketones were found in high quantities only in the leaf volatile oil. aldehydes, alcohols, esters and other compounds were identified in lower q ... | 2012 | 22978236 |
| prevalence of foodborne pathogens in retailed foods in thailand. | the consumption of foodborne pathogens contaminated in food is one of the major causes of diarrheal diseases in thailand. the objective of this study was to evaluate the prevalence and types of contaminating bacteria in retailed foodstuffs in thailand. food from four categories (137 samples total), including meat (51 samples), vegetables (38 samples), fish or seafood (37 samples), and fermented food (11 samples), was purchased randomly from seven different open-markets and seven supermarkets in ... | 2012 | 22953752 |
| production of the antimicrobial peptides caseicin a and b by bacillus isolates growing on sodium caseinate. | the aim of this study was to identify bacillus isolates capable of degrading sodium caseinate and subsequently to generate bioactive peptides with antimicrobial activity. | 2012 | 22642665 |
| development of sandwich enzyme-linked immunosorbent assay for the detection of cronobacter muytjensii (formerly called enterobacter sakazakii). | this study aimed to produce a polyclonal antibody against cronobacter muytjensii (c. muytjensii, formerly called enterobacter sakazakii) and to develop an immunoassay for its detection. the optimum production of rabbit anti-c. muytjensii immunoglobulin g (igg) and chicken anti-c. muytjensii igy was reached in weeks 8 and 9, respectively. purification of rabbit anti-c. muytjensii igg from immunized rabbit sera was accomplished using the caprylic acid and ammonium sulfate precipitation method. as ... | 2012 | 22519814 |
| synthesis, antibacterial, antioxidant activity and qsar studies of novel 2-arylidenehydrazinyl-4-arylthiazole analogues. | a novel series of 2-arylidenehydrazinyl-4-arylthiazole analogues (3a-p) was designed and synthesized in excellent yields using a rapid, simple, efficient methodology. sixteen novel compounds were screened for in vitro antimicrobial activities against eleven bacteria, namely, staphylococcus aureus, listeria monocytogenes, enterococcus faecalis, bacillus subtilis, klebsiella pneumonia, citrobacter freundii, cronobacter sakazakii, salmonella enteritidis, escherichia coli, yersinia pestis, and pseud ... | 2014 | 25450634 |
| in vitro fermentation of fructooligosaccharides with human gut bacteria. | fructooligosaccharides (fos) are one of the most studied prebiotics, selectively stimulating the growth of health-promoting bacteria in the host. however, there is increasing evidence that commensal gut bacteria, such as bacteroides fragilis, clostridium butyricum, enterobacter cloacae, and even the pathogenic escherichia coli ben2908, are also able to metabolize fos in vitro, and in some cases, fos displayed adverse effects. therefore, it is necessary to identify fos-metabolizing species that a ... | 2015 | 25653214 |
| strain-specific probiotic properties of lactic acid bacteria and their interference with human intestinal pathogens invasion. | one of the working mechanisms of probiotic bacteria is their ability to compete with pathogens. to define the probiotic properties of seven lactic acid bacteria (lab) strains, we tested them for survival in simulated gastro-intestinal conditions, antimicrobial activities, co-aggregative abilities, and interferences studies against five human intestinal pathogens (salmonella enteritidis atcc 13076, listeria monocytogenes atcc 7644, escherichia coli o157: h7 atcc 35150, cronobacter sakazakii atcc ... | 2017 | 28286570 |
| evaluation of adhesion properties and antibacterial activities of the infant gut commensal bifidobacterium bifidum prl2010. | bifidobacteria are extensively exploited by the food industry as health-promoting microorganisms. however, very little is known about the molecular mechanisms responsible for these beneficial activities, or the molecular players that sustain their ability to colonize and persist within the human gut. here, we have investigated the enteric adaptation features of the gut commensal bifidobacterium bifidum prl2010, originally isolated from infant feces. this strain was able to survive under gastroin ... | 2013 | 23523946 |
| [identification of zoonotic bacterial pathogens by the maldi tof ms method]. | to verify whether the maldi tof ms method can be used for rapid identification of selected zoonotic bacterial pathogens isolated from various types of materials in the real conditions of routine laboratory work. | 2015 | 26312374 |
| a modified molecular beacons-based multiplex real-time pcr assay for simultaneous detection of eight foodborne pathogens in a single reaction and its application. | foodborne disease outbreaks are often caused by one of the major pathogens. early identification of the causal pathogen is crucial for disease control and prevention. we describe a real-time polymerase chain reaction (rtpcr) assay that can identify, in a single reaction, up to eight common foodborne bacterial pathogens, including salmonella enterica subsp. enterica, listeria monocytogenes, escherichia coli o157, vibrio parahaemolyticus, v. vulnificus, campylobacter jejuni, enterobacter sakazakii ... | 2014 | 24328501 |
| biochemical and biophysical combined study of bicarinalin, an ant venom antimicrobial peptide. | we have recently characterized bicarinalin as the most abundant peptide from the venom of the ant tetramorium bicarinatum. this antimicrobial peptide is active against staphylococcus and enterobacteriaceae. to further investigate the antimicrobial properties of this cationic and cysteine-free peptide, we have studied its antibacterial, antifungal and antiparasitic activities on a large array of microorganisms. bicarinalin was active against fifteen microorganisms with minimal inhibitory concentr ... | 2016 | 27058430 |
| in vitro and in vivo examination of anticolonization of pathogens by lactobacillus paracasei fj861111.1. | very limited information exists on the exclusion of pathogens by probiotics in the gut of the host challenged with pathogens. in this study, we tested probiotic characteristics in vitro and anticolonization ability of lactobacillus paracasei fj861111.1 in mice infected with selected pathogenic microorganisms. the in vitro results indicated that l. paracasei fj861111.1 had a high survival in acidic conditions at ph 2.5 and bile salt concentration at 0.3%, and strong inhibition ability against com ... | 2015 | 26254535 |
| in vitro probiotic characteristics of lactobacillus plantarum zdy 2013 and its modulatory effect on gut microbiota of mice. | lactobacillus plantarum zdy 2013, a novel strain isolated from chinese traditional fermented acid beans, was systematically evaluated for its survival capacity under stress conditions (ph, bile salt, simulated gastrointestinal tract, and antibiotics), production of exopolysaccharide and antagonism against 8 pathogens. its effect on mice gut microbiota was also investigated by quantitative pcr and pcr-denaturing gradient gel electrophoresis. the results showed that zdy 2013 can grow at ph 3.5 and ... | 2015 | 26142853 |
| in vitro fermentation of lactulose by human gut bacteria. | lactulose has been known as a prebiotic that can selectively stimulate the growth of beneficial bifidobacteria and lactobacilli. recent studies have indicated that streptococcus mutans, clostridium perfringens, and faecalibacterium prausnitzii are also able to utilize lactulose. however, the previous studies mainly focused on the utilization of lactulose by individual strains, and few studies were designed to identify the species that could utilize lactulose among gut microbiota. this study aime ... | 2014 | 25340538 |
| diverse profiles of n-acyl-homoserine lactones in biofilm forming strains of cronobacter sakazakii. | the present study investigates the role of quorum sensing (qs) molecules expressed by c. sakazakii in biofilm formation and extracellular polysaccharide expression. the qs signaling was detected using chromobacterium violaceum 026 and agrobacterium tumefaciens ntl4(pzlr4) based bioassay. long chain n-acyl-homoserine lactones (ahls) with c6- c18 chain length were identified using high performance liquid chromatography and liquid chromatography-high resolution mass spectrometry. a higher specific ... | 2016 | 27715454 |
| low diversity bacterial community and the trapping activity of metabolites from cultivable bacteria species in the female reproductive system of the oriental fruit fly, bactrocera dorsalis hendel (diptera: tephritidae). | our goal was to identify the bacteria inhabiting the reproductive system of the female oriental fruit fly, bactrocera dorsalis (hendel), and evaluate the chemotaxis of b. dorsalis to the metabolites produced by the bacteria. based on 16s rrna-based polymerase chain reaction-denaturing gradient gel electrophoresis (pcr-dgge), 18 operational taxonomic units (otus) were assigned to the five bacterial classes betaproteobacteria, alphaproteobacteria, gammaproteobacteria, bacilli and actinobacteria. n ... | 2012 | 22754363 |
| reconstruction of the carotenoid biosynthetic pathway of cronobacter sakazakii baa894 in escherichia coli. | cronobacter sakazakii could form yellow-pigmented colonies. however, the chemical structure and the biosynthetic pathway of the yellow pigments have not been identified. in this study, the yellow pigments of c. sakazakii baa894 were purified and analyzed. the major components of the yellow pigments were confirmed as zeaxanthin-monoglycoside and zeaxanthin-diglycoside. a gene cluster containing seven genes responsible for the yellow pigmentation in c. sakazakii baa894 was identified. the seven ge ... | 2014 | 24466219 |
| simultaneous rapid detection and serotyping of cronobacter sakazakii serotypes o1, o2, and o3 by using specific monoclonal antibodies. | cronobacter sakazakii is a foodborne pathogen associated with rare but often lethal infections in neonates. powdered infant formula (pif) represents the most frequent source of infection. out of the identified serotypes (o1 to o7), o1, o2, and o3 are often isolated from clinical and pif samples. serotype-specific monoclonal antibodies (mabs) suitable for application in enzyme immunoassays (eias) for the rapid detection of c. sakazakii have not yet been developed. in this study, we created specif ... | 2016 | 26850303 |
| extended-spectrum-β-lactamase-producing enterobacteriaceae isolated from vegetables imported from the dominican republic, india, thailand, and vietnam. | to examine to what extent fresh vegetables imported into switzerland represent carriers of extended-spectrum-β-lactamase (esbl)-producing enterobacteriaceae, 169 samples of different types of fresh vegetables imported into switzerland from the dominican republic, india, thailand, and vietnam were analyzed. overall, 25.4% of the vegetable samples yielded one or more esbl-producing enterobacteriaceae, 78.3% of which were multidrug resistant. sixty isolates were obtained: escherichia coli, 26; kleb ... | 2015 | 25724954 |
| synergistic bactericidal effect of simultaneous near-infrared radiant heating and uv radiation against cronobacter sakazakii in powdered infant formula. | the aim of this study was to investigate the synergistic bactericidal effects of the simultaneous application of near-infrared (nir) heating and uv irradiation against cronobacter sakazakii in powdered infant formula and to determine the effect on quality by measuring color changes and performing sensory evaluation. a cocktail of c. sakazakii strains was inoculated into powdered infant formula, followed by nir, uv, and combined nir-uv treatments. the sum of nir and uv inactivation was lower than ... | 2014 | 24413596 |
| a novel bacteriophage targeting cronobacter sakazakii is a potential biocontrol agent in foods. | cronobacter sakazakii is an important pathogen that causes high mortality in infants. due to its occasional antibiotic resistance, a bacteriophage approach might be an alternative effective method for the control of this pathogen. to develop a novel biocontrol agent using bacteriophages, the c. sakazakii-infecting phage cr5 was newly isolated and characterized. interestingly, this phage exhibited efficient and relatively durable host lysis activity. in addition, a specific gene knockout study an ... | 2015 | 26497465 |
| identification of bacterial endophytes associated with traditional medicinal plant tridax procumbens linn. | in traditional medicine, tridax procumbens linn. is used in the treatment of injuries and wounds. the bacterial endophytes (bes) of medicinal plants could produce medicinally important metabolites found in their hosts; and hence, the involvement of bes in conferring wound healing properties to t. procumbens cannot be ruled out. but, we do not know which types of bes are associated with t. procumbens. | 2013 | 24501447 |
| occurrence and characterization of cronobacter spp. in dehydrated rice powder from chinese supermarket. | cronobacter spp. are emerging food-borne pathogens and have been identified as causative agents of meningitis and necrotizing enterocolitis in infants. dehydrated rice is popular with a wide range of people and it is frequently used as a substitute for infant milk powder to baby older than four months. the occurrence of cronobacter spp. was investigated in 1,012 samples of dehydrated rice powder collected from 14 manufacturers in china during 2010 to 2012. the isolates were identified using fusa ... | 2015 | 26132635 |
| genetic determinants of heat resistance in escherichia coli. | escherichia coli aw1.7 is a heat resistant food isolate and the occurrence of pathogenic strains with comparable heat resistance may pose a risk to food safety. to identify the genetic determinants of heat resistance, 29 strains of e. coli that differed in their of heat resistance were analyzed by comparative genomics. strains were classified as highly heat resistant strains, exhibiting a d60-value of more than 6 min; moderately heat resistant strains, exhibiting a d60-value of more than 1 min; ... | 2015 | 26441869 |
| cultivable endophytic bacteria from leaf bases of agave tequilana and their role as plant growth promoters. | agave tequilana weber var. 'azul' is grown for the production of tequila, inulin and syrup. diverse bacteria inhabit plant tissues and play a crucial role for plant health and growth. in this study culturable endophytic bacteria were extracted from leaf bases of 100 healthy agave tequilana plants. in plant tissue bacteria occurred at mean population densities of 3 million cfu/g of fresh plant tissue. three hundred endophytic strains were isolated and 16s rdna sequences grouped the bacteria into ... | 2014 | 25763038 |
| the role of the intestinal microbiota in the pathogenesis of necrotizing enterocolitis. | development of necrotizing enterocolitis (nec) requires a susceptible host, typically a premature infant or an infant with congenital heart disease, enteral feedings and bacterial colonization. although there is little doubt that microbes are critically involved in the pathogenesis of nec, the identity of specific causative pathogens remains elusive. unlike established normal adult gut microbiota, which is quite complex, uniform, and stable, early postnatal bacterial populations are simple, dive ... | 2013 | 23611609 |
| extensive manipulation of caseicins a and b highlights the tolerance of these antimicrobial peptides to change. | caseicins a and b are low-molecular-weight antimicrobial peptides which are released by proteolytic digestion of sodium caseinate. caseicin a (ikhqglpqe) is a nine-amino-acid cationic peptide, and caseicin b (vlnenllr) is a neutral eight-amino-acid peptide; both have previously been shown to exhibit antibacterial activity against a number of pathogens, including cronobacter sakazakii. previously, four variants of each caseicin which differed subtly from their natural counterparts were generated ... | 2012 | 22247170 |
| insights into virulence factors determining the pathogenicity of cronobacter sakazakii. | cronobacter sakazakii is an opportunistic pathogen associated with outbreaks of life-threatening necrotizing enterocolitis, meningitis and sepsis in neonates and infants. the pathogen possesses an array of virulence factors which aid in tissue adhesion, invasion and host cell injury. although the identification and validation of c. sakazakii virulence factors has been hindered by availability of suitable neonatal animal model, various studies has reported outer membrane protein a (ompa) as a pot ... | 2015 | 25950947 |
| hfq plays important roles in virulence and stress adaptation in cronobacter sakazakii atcc 29544. | cronobacter spp. are opportunistic pathogens that cause neonatal meningitis and sepsis with high mortality in neonates. despite the peril associated with cronobacter infection, the mechanisms of pathogenesis are still being unraveled. hfq, which is known as an rna chaperone, participates in the interaction with bacterial small rnas (srnas) to regulate posttranscriptionally the expression of various genes. recent studies have demonstrated that hfq contributes to the pathogenesis of numerous speci ... | 2015 | 25754196 |
| occurrence of yeasts, enterococci and other enteric bacteria in subgingival biofilm of hiv-positive patients with chronic gingivitis and necrotizing periodontitis. | the purpose of this study was to determine the prevalence of enteric bacteria and yeasts in biofilm of 80 hiv-positive patients with plaque-associated gingivitis or necrotizing periodontitis. patients were subjected to extra, intra oral and radiographic examinations. the oral hygiene, bleeding on probing, gingival conditions, and attachment loss were evaluated. clinical specimens were collected from gingival crevices or periodontal pockets, transferred to vmga iii, diluted and transferred to sab ... | 2008 | 24031212 |
| a proposed harmonized lps molecular-subtyping scheme for cronobacter species. | cronobacter are opportunistic pathogens, which cause infections in all age groups. to aid the characterization of cronobacter in foods and environments a harmonized lps identification scheme for molecular serotyping is needed. to this end, we studied 409 cronobacter isolates representing the seven cronobacter species using two previously reported molecular serotyping schemes, described here as mullane-jarvis (m-j) and sun schemes. pcr analysis revealed many overlapping results that were obtained ... | 2015 | 25998813 |
| genotyping and source tracking of cronobacter sakazakii and c. malonaticus isolates from powdered infant formula and an infant formula production factory in china. | cronobacter spp. (formerly defined as enterobacter sakazakii) are opportunistic bacterial pathogens of both infants and adults. in this study, we analyzed 70 cronobacter isolates from powdered infant formula (pif) and an infant formula production facility in china to determine possible contamination routes. the strains were profiled by multilocus sequence typing (mlst), pulsed-field gel electrophoresis (pfge), pcr-based o-antigen serotyping, and ompa and rpob sequence analyses. the isolates were ... | 2015 | 26048942 |
| antibiotic and desiccation resistance of cronobacter sakazakii and c. malonaticus isolates from powdered infant formula and processing environments. | this study evaluated the antimicrobial and desiccation resistance of cronobacter sakazakii and cronobacter malonaticus isolates from powdered infant formula and processing environments. the antimicrobial susceptibility tests showed that the 70 cronobacter strains, representing 19 sequence types, were susceptible to the most of the antibiotics except for amoxicillin-clavulanate, ampicillin, and cefazolin. furthermore, the growth of six c. sakazakii and two c. malonaticus strains from different se ... | 2017 | 28303125 |
| antibacterial activities of plant-derived compounds and essential oils toward cronobacter sakazakii and cronobacter malonaticus. | cronobacter sakazakii and c. malonaticus are opportunistic pathogens that cause infections in children and immunocompromised adults. in the present study, the antibacterial activity of 19 plant-derived compounds, 5 essential oils, and an extract of propolis were assessed against c. sakazakii and c. malonaticus. the effects of most of these antimicrobials have not been reported previously. both strains were susceptible to thymol, carvacrol, thymoquinone, p-cymene, linalool, camphor, citral, eugen ... | 2014 | 25062020 |
| cronobacter spp.--opportunistic food-borne pathogens. a review of their virulence and environmental-adaptive traits. | the genus cronobacter consists of a diverse group of gram-negative bacilli and comprises seven species: cronobacter sakazakii, cronobacter malonaticus, cronobacter muytjensii, cronobacter turicensis, cronobacter dublinensis, cronobacter universalis and cronobacter condimenti. cronobacter are regarded as opportunistic pathogens, and have been implicated in newborn and infant infections, causing meningitis, necrotizing enterocolitis and bacteraemia or sepsis. cronobacter virulence is believed to b ... | 2014 | 24878566 |
| presence of ampc beta-lactamases, csa-1, csa-2, cma-1, and cma-2 conferring an unusual resistance phenotype in cronobacter sakazakii and cronobacter malonaticus. | here we describe the presence of two very similar but unusual variants of ampc cephalosporinase in each cronobacter sakazakii and c. malonaticus isolates conferring resistance exclusively to first generation cephalosporins. during a survey on the antibiotic resistance patterns of c. sakazakii and c. malonaticus strains isolated from a milk powder production facility, originally two different phenotypes regarding the susceptibility/resistance for the two beta-lactam antibiotics ampicillin (amp) a ... | 2014 | 24568164 |
| cronobacter carriage in neonate and adult intestinal tracts. | a total of 7 cronobacter strains were isolated from 703 fecal samples collected in jinan from june 13 to december 30, 2011, with the positive rate of cronobacter spp. being 1.0% (95% confidence interval 0.6%-1.4%). three cronobacter sakazakii stains were isolated from 157 fecal samples of healthy neonates (95% confidence interval 0.4%-5.5%). this number was slightly higher than that isolated from 273 fecal samples of healthy adults, in which 1 strain of c. sakazakii and 1 strain of cronobacter m ... | 2013 | 24215882 |
| first clinical isolates of cronobacter spp. (enterobacter sakazakii) in argentina: characterization and subtyping by pulsed-field gel electrophoresis. | cronobacter species are opportunistic pathogens associated with severe infections in neonates and immunocompromised infants. from january 2009 through september 2010, two cases of neonatal infections associated with cronobacter malonaticus and one case associated with cronobacter sakazakii, two of them fatal, were reported in the same hospital. these are the first clinical isolates of cronobacter spp. in argentina. the objective of this work was to characterize and subtype clinical isolates of c ... | 2013 | 24165138 |
| structural and genetic relationships of closely related o-antigens of cronobacter spp. and escherichia coli: c. sakazakii g2594 (serotype o4)/e. coli o103 and c. malonaticus g3864 (serotype o1)/e. coli o29. | o-antigen (o-polysaccharide) variation is the basis for bacterial serotyping and is important in bacterial virulence and niche adaptation. in this work, we present structural and genetic evidences for close relationships between the o-antigens of the cronobacter spp. and escherichia coli. cronobacter sakazakii g2594 (serotype o4) and cronobacter malonaticus g3864 (serotype o1) are structurally related to those of e. coli o103 and o29, respectively, and some other members of the enterobacteriacea ... | 2015 | 25555751 |
| multilocus sequence typing of cronobacter strains isolated from retail foods and environmental samples. | cronobacter spp. are bacterial pathogens that affect children and immunocompromised adults. in this study, we used multilocus sequence typing (mlst) to determine sequence types (sts) in 11 cronobacter spp. strains isolated from retail foods, 29 strains from dust samples obtained from vacuum cleaners, and 4 clinical isolates. using biochemical tests, species-specific polymerase chain reaction, and mlst analysis, 36 strains were identified as cronobacter sakazakii, and 6 were identified as cronoba ... | 2015 | 25974656 |
| biofilms in bioremediation and wastewater treatment: characterization of bacterial community structure and diversity during seasons in municipal wastewater treatment process. | the bacterial community structure and diversity were assessed at the scale of rotating biodisk procedure (rb) in a semi-industrial pilot plant. as well, the salmonella community was particularly monitored, and the effects of ultraviolet (uv-c254) on the bacterial community were studied. the identification of dominant bacteria revealed the presence of beneficial and useful species that could play an important role in the process of wastewater purification. several species as enterobacter agglomer ... | 2017 | 27878485 |
| label-free 3d ag nanoflower-based electrochemical immunosensor for the detection of escherichia coli o157:h7 pathogens. | it is highly desirable to develop a rapid and simple method to detect pathogens. combining nanomaterials with electrochemical techniques is an efficient way for pathogen detection. herein, a novel 3d ag nanoflower was prepared via a biomineralization method by using bovine serum albumin (bsa) as a template. it was adopted as a sensing interface to construct an electrochemical bacteria immunosensor for the rapid detection of foodborne pathogens escherichia coli (e. coli) o157:h7. bacterial antibo ... | 2016 | 27858378 |
| characterization of cronobacter spp. isolated from food of plant origin and environmental samples collected from farms and from supermarkets in the czech republic. | the cronobacter genus (previously known as enterobacter sakazakii) comprises seven species (cronobacter sakazakii, cronobacter malonaticus, cronobacter muytjensii, cronobacter turicensis, cronobacter dublinensis, cronobacter universalis and cronobacter condimenti)which cause serious infections in neonates and immunocompromised people.most of the documented outbreaks of these bacteria have been associated with consumption of contaminated powdered infant formula. the plant environment is considere ... | 2016 | 26513253 |
| contribution of the thermotolerance genomic island to increased thermal tolerance in cronobacter strains. | cronobacter spp. are opportunistic pathogens associated with serious infections in neonates. increased stress tolerance, including the thermotolerance of some cronobacter strains, can promote their survival in production facilities and thus raise the possibility of contamination of dried infant formula which has been identified as a potential source of infection. some cronobacter strains contain a genomic island, which might be responsible for increased thermotolerance. by analysis of cronobacte ... | 2016 | 26748923 |
| duplex real-time pcr method for the differentiation of cronobacter sakazakii and cronobacter malonaticus. | cronobacter sakazakii and cronobacter malonaticus are the most common species of cronobacter , so it is necessary to detect the two species as soon as possible in surveillance programs. we developed a real-time pcr method for identifying c. sakazakii and c. malonaticus from the genus cronobacter . in this study, the two pairs of primers and probes were designed, targeting 16s rrna and fusa, respectively. the specificity of the real-time pcr assay was validated with 112 strains of cronobacter , i ... | 2017 | 28221877 |
| analysis and characterization of proteins associated with outer membrane vesicles secreted by cronobacter spp. | little is known about secretion of outer membrane vesicles (omvs) by cronobacter. in this study, omvs isolated from cronobacter sakazakii, cronobacter turicensis, and cronobacter malonaticus were examined by electron microscopy (em) and their associated outer membrane proteins (omp) and genes were analyzed by sds-page, protein sequencing, blast, pcr, and dna microarray. em of stained cells revealed that the omvs are secreted as pleomorphic micro-vesicles which cascade from the cell's surface. sd ... | 2017 | 28232819 |
| surveillance and characterisation of cronobacter spp. in czech retail food and environmental samples. | cronobacter spp. (formerly enterobacter sakazakii) are emerging, opportunistic pathogens that are linked with food-borne infections in neonates and infants. in the present study, 291 samples of food, 36 samples from a dairy farm and 140 samples of dust from vacuum cleaners were examined for the presence of cronobacter spp. using chromogenic media and biochemical tests. altogether, 72 cronobacter spp. strains were isolated in accordance with the reference standard čsn p iso/ts 22964 (2006). no cr ... | 2014 | 23873391 |
| international life science institute north america cronobacter (formerly enterobacter sakazakii) isolate set. | foodborne pathogen isolate collections are important for the development of detection methods, for validation of intervention strategies, and to develop an understanding of pathogenesis and virulence. we have assembled a publicly available cronobacter (formerly enterobacter sakazakii) isolate set that consists of (i) 25 cronobacter sakazakii isolates, (ii) two cronobacter malonaticus isolates, (iii) one cronobacter muytjensii isolate, which displays some atypical phenotypic characteristics, bioc ... | 2013 | 23317855 |
| comparison of methods for the microbiological identification and profiling of cronobacter species from ingredients used in the preparation of infant formula. | cronobacter spp. (formerly enterobacter sakazakii) can be isolated from a wide range of foods and environments, and its association with neonatal infections has drawn considerable attention from regulatory authorities. the principle route of neonatal infection has been identified as the ingestion of contaminated infant formula. a number of methods have been developed to identify cronobacter spp., however these were before the most recent (2012) taxonomic revision of the genus into seven species. ... | 2013 | 23089182 |
| occurrence of cronobacter spp. in retail foods. | to study the occurrence of cronobacter spp. in foods and to investigate the phenotypic properties of the strains isolated. | 2012 | 22443682 |
| structural derivation of lipid a from cronobacter sakazakii using tandem mass spectrometry. | cronobacter sakazakii is a gram-negative opportunistic pathogen that can cause necrotizing enterocolitis, bacteremia, and meningitis. lipid a, the glycolipid membrane anchor of lipopolysaccharide (lps), is a potential virulence factor for c. sakazakii. given the potential importance of this molecule in infection and virulence, structural characterization of lipid a was carried out. | 2016 | 27502448 |
| recovery estimation of dried foodborne pathogens is directly related to rehydration kinetics. | drying is a common process which is used to preserve food products and technological microorganisms, but which is deleterious for the cells. the aim of this study is to differentiate the effects of drying alone from the effects of the successive and necessary rehydration. rehydration of dried bacteria is a critical step already studied in starter culture but not for different kinetics and not for pathogens. in the present study, the influence of rehydration kinetics was investigated for three fo ... | 2016 | 27494169 |
| immunochromatographic strip assay for detection of cronobacter sakazakii in pure culture. | cronobacter sakazakii (c. sakazakii) is a foodborne pathogen, posing a high risk of disease to infants and immunocompromised individuals. in order to develop a quick, easy, and sensitive assay for detecting c. sakazakii, a rabbit anti-c. sakazakii immunoglobulin g (igg) was developed using sonicated cell protein from c. sakazakii. the developed anti-c. sakazakii (igg) was of good quality and purity, as well as species-specific. the developed rabbit anti-c. sakazakii igg was attached to the surfa ... | 2016 | 27470276 |
| new advances in infant feeding: new products and novel technologies. | breastfeeding is the best way to provide ideal food for infants. however, there are many situations in which breastfeeding is unviable (maternal illness, hygiene problems, intravenous feeding requirement, storage). safe, efficient, and nutritious products should be offered by the industry to mothers and healthcare professionals as an alternative. | 2017 | 28355995 |
| prevalence and antimicrobial susceptibility of enterobacteriaceae isolated from retail pepper in vietnam. | to investigate the microbial quality of retail pepper in vietnam, the enumeration and detection of enterobacteriaceae and the screening of cefotaxime (ctx)-resistant coliforms were performed by using 84 commercial samples. although enterobacteriaceae were isolated from 78 samples, the number of enterobacteriaceae was lower than 1.0 log cfu/g in 46 samples. for the detection of enterobacteriaceae with the international organization for standardization methods, salmonella spp., escherichia coli , ... | 2017 | 28350183 |
| publisher's expression of concern: lactobacillus bulgaricus prevents intestinal epithelial cell injury caused by enterobacter sakazakii-induced nitric oxide both in vitro and in the newborn rat model of necrotizing enterocolitis. | | 2017 | 28336717 |
| drying parameters greatly affect the destruction of cronobacter sakazakii and salmonella typhimurium in standard buffer and milk. | salmonella typhimurium and cronobacter sakazakii are two foodborne pathogens involved in neonatal infections from milk powder and infant formula. their ability to survive in low-moisture food and during processing from the decontamination to the dried state is a major issue in food protection. in this work, we studied the effects of the drying process on salmonella typhimurium and cronobacter sakazakii, with the aim of identifying the drying parameters that could promote greater inactivation of ... | 2017 | 27889170 |