| necrotizing ulcerative gingivitis, periodontitis, and stomatitis: clinical staging and predisposing factors. | necrotizing ulcerative gingivitis (nug), necrotizing ulcerative periodontitis (nup), and necrotizing stomatitis (ns), collectively termed necrotizing gingivostomatitis (ng), represent a dramatic, but rare oral infection associated with diminished systemic resistance, including hiv infection. over a 5-year period, 68 consecutive ng patients from a population with known hiv status were evaluated and treated. lesions were staged (modified pindborg), and clinical findings and predictor variables wer ... | 1995 | 8558402 |
| oral microbiota in subjects with a weak or strong response in experimental gingivitis. | the purpose of the present study was to examine the composition of the oral microbiota in subjects who had previously demonstrated to develop either a weak or strong response to experimental gingivitis. for this study, subjects were selected from a pool of 25 individuals who had participated twice in an experimental gingivitis trial. out of these 25 panellists, 6 subjects were selected who had developed 2x a weak gingival inflammatory response and 7 subjects who had developed 2x a strong gingiva ... | 1995 | 8583022 |
| [subgingival distribution of periodontopathic bacteria in periodontic patients and susceptibility of these bacteria to minocycline-hcl]. | the present study was carried out to examine the distribution of six periodontopathic bacteria in deep periodontal pockets and to reconfirm the effect of periocline on these periodontopathic bacteria. samples from sixty-two periodontal pockets were collected at pocket depths of over 4 mm in twenty-one periodontitis patients aged 43 to 75 years. after sampling, periocline was applied topically to the selected pockets once a week for four weeks and reexamined. the detected rates of the periodontop ... | 1995 | 8583161 |
| [effects of the prevotella intermedia culture filtrate and short-chain fatty acids on human polymorphonuclear neutrophil functions]. | anaerobes, in particular prevotella spp., are the predominant bacteria present in mixed respiratory infections. to study the virulence factor of prevotella intermedia, we examined the effects of the culture filtrate of p. intermedia on the bactericidal activity of human polymorphonuclear neutrophils (pmns). the culture filtrate significantly inhibited the phagocytic killing and the intracellular killing of staphylococcus aureus by pmns (p < 0.05), but the phagocytosis of pmns was not affected. t ... | 1995 | 8586886 |
| confirmation of the species prevotella intermedia and prevotella nigrescens. | the elevation of the two genotypes of prevotella intermedia to species rank as p. intermedia and prevotella nigrescens has increased the need for reliable differentiation between the two taxa. in this study, 53 strains, including strains whose species affiliations were known as well as fresh dental plaque isolates, were subjected to a multilocus enzyme electrophoretic analysis, dna analyses in which we used whole genomic dna, rrna sequences, and an oligonucleotide specific for the former p. inte ... | 1995 | 8590668 |
| postoperative healing complications associated with gore-tex periodontal material. part i. incidence and characterization. | guided tissue regeneration procedures with gore-tex periodontal material are associated with a unique set of postoperative healing characteristics. five healing complications are described in this study examining 102 sites. the occurrence of pain and purulence were the most common. in purulent sites, the majority of bacteria cultured were actinomyces and streptococcus spp. resistance to antibiotics was common. prevention and treatment of the healing complications are discussed. | 1995 | 8593986 |
| antimicrobial and toxic effects of established and potential root canal irrigants. | three dilutions of the commonly used irrigant sodium hypochlorite (0.5%, 2.5%, and 5.25%) and the potential irrigants peridex, chlorhexidine gluconate (0.12%), and therasol were tested. alcohol and saline served as controls also. the in vitro portion of this study used four different microorganisms (streptococcus mutans, peptostreptococcus micros, prevotella intermedius, and porphyromonas gingivalis) to determine antimicrobial effect. presterilized 7-mm diameter whatman paper disks soaked with t ... | 1995 | 8596073 |
| porphyromonas gingivalis outer membrane vesicles promote bacterial resistance to chlorhexidine. | porphyromonas gingivalis has been frequently associated with some types of periodontal diseases and possesses various mechanisms favoring the pathogenic process. it has been recently observed that vesicles elaborated by p. gingivalis are able to protect bacteria from the bactericidal activity of human serum. the aim of the present investigation was to evaluate the ability of vesicles from p. gingivalis in protecting oral bacteria against chlorhexidine. data indicate that vesicles released by p. ... | 1995 | 8596676 |
| microbial alterations in supragingival dental plaque in response to a triclosan-containing dentifrice. | a total of 325 subjects were entered into a double-blind, stratified 2-treatment clinical study that examined the effects of a dentifrice containing 0.3% triclosan, 2% gantrez copolymer and 0.243% sodium fluoride on supragingival dental plaque and gingivitis. a subset of 159 subjects including 72 men and 87 women participated in the microbiological component of this study, which was designed to detect shifts in supragingival bacterial species in response to triclosan. subjects were divided into ... | 1995 | 8602338 |
| cell envelope and cell wall immunization of macaca fascicularis: effect on the progression of ligature-induced periodontitis. | the nonhuman primate, macaca fascicularis, was used to study the role of immunization with selected members of the periodontopathic microbiota in the longitudinal progression of ligature-induced periodontitis. animals were immunized with cell envelope antigens prepared from porphyromonas gingivalis and prevotella intermedia, and a mixture prepared from fusobacterium nucleatum, campylobacter rectus, and actinomyces viscosus. serum immunoglobulin g (igg), igm and iga isotype antibodies increased s ... | 1995 | 8602339 |
| viability of four putative periodontal pathogens and enteric rods in the anaerobic transport medium vmga iii. | the aim of this study was to determine the survival in vmga iii of porphyromonas gingivalis, prevotella intermedia, fusobacterium nucleatum, actinobacillus actinomycetemcomitans and enteric rods in laboratory cultures as well as in subgingival plaque samples. laboratory strains of the 4 putative periodontal pathogens and escherichia coli were used in the laboratory part of this study. also, 31 subgingival plaque samples were obtained from 22 periodontal patients and stored in vmga iii. each samp ... | 1995 | 8602345 |
| colorimetric microtiter plate based assay for detection and quantification of amplified actinobacillus actinomycetemcomitans dna. | we developed a colorimetric microtiter plate-based assay for the detection and quantification of polymerase chain reaction-amplified dna fragment specific for actinobacillus actinomycetemcomitans. we amplified the 396-bp leukotoxin-specific dna fragment by using two oligonucleotide primers, one carrying a biotin group at the 5' end and another one with a digoxigenin at the 5' end. following amplification, the biotinylated polymerase chain reaction products were applied to a microtiter well preco ... | 1995 | 8602346 |
| prevotella intermedia fimbriae mediate hemagglutination. | our earlier studies demonstrated that clinical strains of prevotella intermedia, isolated from human periodontal pockets, possess various types of fimbria (surface appendages) as determined ultrastructurally. these bacteria have the ability to agglutinate selected mammalian erythrocytes. hemagglutinating activity exhibited by these cells may be attributable to these surface structures. strain 17, which possess fimbriae of 8 nm in diameter and readily agglutinates human, monkey, sheep, rabbit, an ... | 1996 | 8604254 |
| periodontal healing and periopathogenic microflora in smokers and non-smokers. | the aim of the present study was to monitor the clinical and microbiological effects of non-surgical therapy in smokers and non-smokers. the subject material included 32 patients (age range 32-61 years), 11 men and 21 women with moderate to severe periodontitis. 17 patients were smokers ( > or = 15 cigarettes/day) and 15 non-smokers. all patients were subjected to non-surgical periodontal therapy performed by a dental hygienist. periodontal variables (plaque index, gingival index and probing dep ... | 1995 | 8613564 |
| interleukin-8 gene expression by human dental pulp fibroblast in cultures stimulated with prevotella intermedia lipopolysaccharide. | interleukin (il)-8 mrna expression was investigated in human dental pulp fibroblast cultures after stimulation with lipopolysaccharide (lps) prepared from prevotella intermedia and inflammatory cytokines. the expression of il-8 mrna and the release of il-8 induced by p. intermedia lps in pulpal fibroblast cultures were detected by northern blot analysis and elisa, respectively. the sufficient concentration of p. intermedia lps on the il-8 mrna expression was 0.1 microgram/ml in pulpal fibroblast ... | 1996 | 8618087 |
| bacteriological diagnosis of periodontal disease. | dental plaque bacteria cause virtually all the forms of inflammatory periodontal disease. periodontitis is caused by the specific periodontopathic bacteria, which induce destruction of connective tissue attachment and adjacent alveolar bone. examinations to identify the infections by porphyromonas gingivalis, actinobacillus actinomycetemcomitans, prevotella intermedia, campylobacter rectus, bacteroides forsythus, fusobacterium nucleatum, eikenella corrodens and treponema denticola have recently ... | 1994 | 8620588 |
| oral health status related to subgingival bacterial flora and sex hormones in saliva during pregnancy. | the purpose of this study was to investigate oral health status in relation to subgingival microflora and sex hormone concentrations in saliva during pregnancy. oral health examinations were performed on 39 subjects: of 19 pregnant women (mean age 28.5), and 8 women in the fifth post-partum month (mean age 27.1), and 12 non-pregnant women (mean age 22.9). periodontal conditions of bleeding on probing, redness, swelling, and probing depth were examined. subgingival microbial compositions were exa ... | 1994 | 8620592 |
| studies into the microbial spectrum of apical periodontitis. | this study examined the variety of obligate and facultative anaerobic bacterial species recovered from cases of acute apical periodontitis. a total of 19 root canal samples and 24 periapical granuloma samples were taken from patients suffering pain and discomfort. bacteria were identified by applying the following techniques: culturing on various media, gram-staining and using commercially available biochemical test strips. in addition, prevotella intermedia and porphyromonas endodontalis were d ... | 1995 | 8626206 |
| immune response to fusobacterium nucleatum and prevotella intermedia in patients with infectious mononucleosis. | the role of four oral flora organisms (fusobacterium nucleatum, prevotella intermedia, porphyromonas gingivalis and actinobacillus actinomycetemcomitans) was investigated in 22 patients with infectious mononucleosis. immunoglobulin-g class antibody titres to these organisms were measured by enzyme-linked immunosorbent assay. serum levels in the patients were determined at day 1 and 42-56 days later. significantly higher antibody levels to f. nucleatum and pr. intermedia were found in the second ... | 1996 | 8642574 |
| repopulation of periodontal pockets by microbial pathogens in the absence of supportive therapy. | this clinical study evaluated the reinfection incidence by actinobacillus actinomycetemcomitans (aa), porphyromonas gingivalis (pg), and prevotella intermedia (pi) in periodontal pockets following scaling and root planing (srp) and intra-pocket irrigation with antimicrobial agents in a patient population who did not receive supportive maintenance therapy. the number of target organisms was determined utilizing dna probes. forty-one (41) inflamed pockets > or = 5 mm with attachment loss and conta ... | 1996 | 8667133 |
| prevotella intermedia and prevotella nigrescens serotypes, ribotypes and binding characteristics. | type strains and 62 clinical isolates of prevotella intermedia and prevotella nigrescens were typed with the use of genomic dna fingerprints and rrna gene probes. the strains were further serotyped with monoclonal antibodies and characterized with sds-page, enzymatic activities, hemolysis and hemagglutination and coaggregation with streptococcus and actinomyces spp. p. intermedia and p. nigrescens were found to have distinct ribotype patterns which correspond to previously defined serotyupes i a ... | 1996 | 8674976 |
| microbiological and serological investigations of oral lesions in papillon-lefèvre syndrome. | microbiological and serological (enzyme linked immunosorbent assay) investigations were carried out, including karyotyping, on two asian children with papillon-lefèvre syndrome. in case 1, a girl aged four years, the most prevalent putative periodontopathogens were eikenella corrodens, fusobacterium nucleatum, porphyromonas gingivalis, prevotella intermedia (deciduous dentition) and bacteroides gracilis, e corrodens and f nucleatum (permanent dentition). in case 2, a boy aged nine years, they we ... | 1996 | 8675741 |
| effect of initial therapy on dynamics of immunogloblin g levels to some periodontopathic bacteria in serum and gingival crevicular fluid. | using enzyme-linked immunosorbent assay, the igg antibody levels to specific gram-negative periodontopathic bacteria in serum of 21 adult periodontitis patients and the gingival crevicular fluid (gcf) from 42 sites with destructive lesions and 21 healthy sites were evaluated before and after initial preparation. porphyromonas gingivalis, bacteroides forsythus, prevotella intermedia, prevotella nigrescens, campylobacter rectus, treponema denticola and actinobacillus actinomycetemcomitans in subgi ... | 1994 | 8689734 |
| collagen-binding activity of prevotella intermedia measured by a microtitre plate adherence assay. | the ability of prevotella intermedia to bind type i collagen was investigated. a simple method in which bacterial cells were allowed to attach to collagen-coated microtitre plate wells was used to characterize the activity. all strains of p. intermedia tested, as well as those of the closely related species prevotella nigrescens, showed a capacity to attach to the collagen film. exponential-phase cultures of p. intermedia demonstrated a greater binding capacity than older cells. attachment to th ... | 1996 | 8704994 |
| the effect of a single mechanical treatment on the subgingival microflora in patients with hiv-associated gingivitis. | the purpose of the present study was to investigate the effect of a single episode of scaling and root planing on clinical periodontal parameters and on the subgingival microflora in human immunodeficiency virus (hiv)-positive and hiv-negative subjects. 13 subjects participated and were clinically scored at days -7, 7, 30 and 90. 7 subjects were infected with hiv and presented an hiv-associated gingivitis. 6 subjects were hiv-negative with at least 12 teeth affected by conventional gingivitis. n ... | 1996 | 8707976 |
| comparison of the pro-inflammatory cytokine-stimulating activity of the surface-associated proteins of periodontopathic bacteria. | saline extraction of the periodontopathic bacterium, actinobacillus actinomycetemcomitans, releases surface-associated material (sam), a complex mixture of proteins and carbohydrates with potent biological actions on isolated bone and on various mammalian cell populations. in this study, the relative ability of the sam from 5 organisms, implicated in the pathology of periodontal disease, to stimulate human mesenchymal and myelomonocytic cells to synthesize the proinflammatory cytokines - interle ... | 1996 | 8708939 |
| clinical and microbiological observations of early polytetrafluoroethylene membrane exposure in guided bone regeneration. case reports in primates. | premature membrane exposure at one week is described in 3 macaca mulatta monkeys as part of a sequence of experiments on guided bone regeneration. clinical sequelae include redness, edema, and tissue slough. bacteroides fragilis, streptococcus pneumoniae, prevotella intermedia, and staphylococcus intermedius were detected at all prematurely exposed sites. pseudomonas maltophilia, strept, pneumoniae, and p. intermedia were the predominant organisms detected and consisted of more than 10% of the t ... | 1996 | 8708956 |
| the effect of periodontal treatment on periodontal bacteria on the oral mucous membranes. | the aim of this study was to investigate the effect of conventional periodontal treatment on the prevalence of actinobacillus actinomycetemcomitans, porphyromonas gingivalis, and prevotella intermedia on oral mucous membranes in patients with periodontitis. fifteen patients were selected with moderate to severe periodontitis (mean age: 39.8 years, range: 26 to 58). from each patient the 4 deepest sites with bleeding on probing were selected for clinical and microbiological evaluation. at baselin ... | 1996 | 8724705 |
| analytical performance of an immunologic-based periodontal bacterial test for simultaneous detection and differentiation of actinobacillus actinomycetemcomitans, porphyromonas gingivalis, and prevotella intermedia. | the analytical performance of a membrane-based immunoassay for the simultaneous detection and differentiation of actinobacillus actinomycetemcomitans, porphyromonas gingivalis, and prevotella intermedia (including prevotella nigrescens) was investigated. positive reactions were observed for 71 of 71 reference strains and recent oral isolates of a. actinomycetemcomitans, p. gingivalis, and p. intermedia. no cross-reactivity was observed with 39 other common oral and environmental species. the spe ... | 1996 | 8724708 |
| long-term stability of class ii furcation defects treated with barrier membranes. | the present longitudinal study was designed to explore the long-term efficacy of guided tissue regeneration (gtr) in class ii furcation defects and establish the factors that might be responsible for modifying this response. subjects with two or more mandibular molars, one of which had class ii furcation defects, received the hygienic phase of therapy followed by baseline clinical measurements and subgingival plaque sampling. gtr procedure was performed in furcation defect sites using expanded p ... | 1996 | 8724711 |
| antagonistic effect of oral bacteria towards treponema denticola. | this study was designed to isolate oral bacteria exhibiting antagonism towards treponema denticola and to characterize the inhibitory activity. eleven bacterial isolates obtained from subgingival sites and identified as either staphylococcus aureus or streptococcus mutans were found to inhibit the growth of t. denticola. when the activity spectra of these isolates were analyzed, two additional periodontopathogens (porphyromonas gingivalis and prevotella intermedia) were found to be affected, whe ... | 1996 | 8727911 |
| histological and microbiological aspects of ligature-induced periodontitis in beagle dogs. | this study was designed to investigate, using 6 beagle dogs, the levels of selected putative pathogens in healthy sites, in gingivitis sites, and in sites with histologically confirmed attachment loss. levels of attachment loss increased with increasing periods of ligation and reached a maximum of 0.15 mm at 57 days. both histological attachment level and histological pocket depth were found to vary significantly with health/disease status (p < 0.0001). higher numbers of total colony-forming uni ... | 1996 | 8739161 |
| response to periodontal therapy in patients with high or low levels of p. gingivalis, p. intermedia, p. nigrescens and b. forsythus. | in a previous study, subjects receiving either adjunctive tetracycline or augmentin showed, on average, more attachment level gain 10 months post-therapy than subjects receiving either ibuprofen or a placebo, although some subjects in each treatment group showed loss of attachment post-therapy. since differences in treatment response might have been due to differences in the subgingival microbiota, the response to different therapies in subjects with different pre-therapy subgingival microbiotas ... | 1996 | 8739165 |
| utility of 5 major putative periodontal pathogens and selected clinical parameters to predict periodontal breakdown in patients on maintenance care. | the predictive utility of 5 major putative periodontopathic microbial species, "superinfecting" organisms, and several clinical periodontal parameters were assessed relative to periodontitis recurrence over a 12-month period in 78 treated adult patients participating in a 3-month maintenance care program. at baseline, pooled subgingival microbial samples were collected from each patient, and whole-mouth evaluations of probing depth, relative periodontal attachment level, furcation involvement, a ... | 1996 | 8739166 |
| the effect of age on the development of gingivitis. clinical, microbiological and histological findings. | in the present "experimental gingivitis" study, the response of the marginal gingiva to plaque formation was studied in one group of young subjects, 20-25 years of age, and in one group of older subjects, aged 65-80 years. during a 4-week period, all subjects received a series of professional tooth cleaning to establish healthy gingival conditions. a baseline examination (day 0) included assessments of plaque and gingivitis. sites that were examined (the experimental sites) included the mesio-pa ... | 1996 | 8739171 |
| systemic antimicrobial treatment and guided tissue regeneration. clinical and microbiological effects in furcation defects. | the purpose of this investigation was to study the microbiota associated with furcation-involved teeth before and after treatment by the guided tissue regeneration procedure (gtr) with non-resorbable eptfe membranes, and to evaluate the benefit of additional systemic antimicrobial therapy (ornidazole). each of 10 patients contributed 1 pair of bilateral mandibular molars with comparable furcation defects. 5 defects were treated with a membrane and the active drug, 5 were treated without a membra ... | 1996 | 8739172 |
| [treatment of diversion colitis with short-chain fatty acids. bacteriological study]. | bacterial imbalance may be involved in the pathogenesis of diversion colitis, via diminished production of short chain fatty acids. the aim of the study was to evaluate the effectiveness of short chain fatty acids on microbial flora of patients with diversion colitis and to compare this flora to the microbial flora of controls. | 1995 | 8746044 |
| effect of titanium on selected oral bacterial species in vitro. | titanium granules were tested for their antibacterial effect on strains of streptococcus sanguis, streptococcus mitis, actinomyces naeslundii, haemophilus parainfluenzae, fusobacterium spp. and prevotella intermedia in comparison with amalgam and two of its components, copper and tin. glass beads were used as controls. the number of viable bacteria was estimated in samples exposed to the various materials for 1, 3, 6 and 24 h, respectively, and the viable counts were related to the baseline valu ... | 1995 | 8747674 |
| colonization by actinobacillus actinomycetemcomitans, porphyromonas gingivalis and prevotella intermedia in adult periodontitis patients as detected by the antibody-based evalusite test. | studies were performed to evaluate the detection of disease-associated bacterial colonization in adult periodontitis patients by the antibody-based evalusite testtm (eastman kodak company). the association of test results with disease was assessed by collecting 104 duplicate subgingival plaque samples from 26 patients. samples were tested for actinobacillus actinomycetemcomitans, porphyromonas gingivalis and prevotella intermedia using both microbiological culture and the immunoassay test. the s ... | 1996 | 8783055 |
| [in vitro activities of sulopenem, a new parenteral penem, against anaerobes]. | in vitro activities of sulopenem, a novel parenteral penem, was compared with those of imipenem, flomoxef, cefuzonam, cefoperazone and sulbactam/ampicillin against 66 reference strains (19 genera, 61 species) and 392 recent clinical isolates of anaerobic bacteria and fastidious aerobic bacteria. sulopenem had a very broad spectrum against anaerobic bacteria. in general, this compound was active against anaerobic reference strains with mics of < or = 0.78 micrograms/ml, while being the least acti ... | 1996 | 8786627 |
| treatment of periodontal disease based on microbiological diagnosis. relation between microbiological and clinical parameters during 5 years. | the purpose of this study was to assess the clinical effect of treatment aimed to suppress actinobacillus actinomycetemcomitans and porphyromonas gingivalis below detection level and prevotella intermedia below 5% of the cultivable periodontal pocket flora. sixteen patients and a total of 111 periodontal pockets with probing depth > or = 6 mm were included in the study. twelve patients and a total of 77 sites completed the 5-year study. the results demonstrated clinical improvement of probing de ... | 1996 | 8794965 |
| lipopolysaccharides from porphyromonas gingivalis, prevotella intermedia and actinobacillus actinomycetemcomitans promote osteoclastic differentiation in vitro. | bacterial lipopolysaccharides possess bone-resorbing activity. here, lipopolysaccharides from three putative periodontopathic bacteria were examined for effects on osteoclast-like cell formation of bone marrow cells from lipopolysaccharide-responsive c3h-hen and non-responsive c3h/hej mice. the bone marrow cells were cultured with or without various doses of lipopolysaccharide in the presence of 1,25-dihydroxyvitamin d3 and dexamethasone. these lipopolysaccharide preparations significantly incre ... | 1996 | 8809306 |
| binding and degradation of lactoferrin by porphyromonas gingivalis, prevotella intermedia and prevotella nigrescens. | the ability of laboratory and clinical strains of porphyromonas gingivalis, prevotella intermedia and prevotella nigrescens to bind and to degrade lactoferrin (lf) has been assessed. lf bound readily to whole cells of each species apparently via high-affinity site and one or more low-affinity sites. p. gingivalis showed a lower affinity for lf than the other two species (p < 0.001). virtually all strains of p. gingivalis completely degraded lf under the conditions employed, whereas p. intermedia ... | 1996 | 8809549 |
| phototoxicity of argon laser irradiation on biofilms of porphyromonas and prevotella species. | species of prevotella (pr.) and porphyromonas (po.) and other microorganisms were cultivated as biofilms on agar medium and examined for their susceptibility to argon laser irradiation (continuous mode; wavelengths, 488-514 nm; fluences, 20-200 j cm(-2)). fluences of 35 to 80 j cm(-2) inhibited biofilm growth in po. endodontalis, po. gingivalis, pr. denticola, pr. intermedia, pr. melaninogenica and pr. nigrescens. a fluence of 70 j cm(-2) did not affect biofilm growth in species of bacillus, can ... | 1996 | 8810530 |
| effects of cyclosporine a on plasma cells in experimental gingivitis in dogs. | the aim of the present investigation was to evaluate the effect of cyclosporine a (csa) on the inflammatory lesion formed in the gingival tissues during de novo plaque formation. 5 beagle dogs were used. on day 0, all teeth of the 5 dogs were scaled and polished. a 6-week period of plaque control including daily tooth cleaning with toothbrush and dentifrice was initiated. a clinical examination regarding plaque and gingivitis was performed, and the plaque control measures were abandoned on the r ... | 1996 | 8811468 |
| chemiluminescent assay of alkaline phosphatase in human gingival crevicular fluid: investigations with an experimental gingivitis model and studies on the source of the enzyme within crevicular fluid. | the purpose of this study was to investigate how levels of gingival crevicular fluid (gcf) alkaline phosphatase (alp) change in relation to levels of plaque and gingival inflammation in 20 adults during a 21 day period of experimental gingivitis. the source of alp within gcf was also investigated using a repeat sampling protocol; by determining enzyme levels derived from 30 putative periodontal pathogenic and non-pathogenic species; and by examining inhibition profiles from a variety of host and ... | 1996 | 8811480 |
| source of suspected periodontal pathogens re-emerging after periodontal treatment. | to clarify the source of re-emerging periodontal pathogens after treatment, we compared the ribotypes of actinobacillus actinomycetemcomitans, porphyromonas gingivalis, prevotella intermedia/prevotella nigrescens group and campylobacter rectus before and after treatment in 7 periodontitis patients and in 6 of the spouses. the patients harbored a. actinomycetemcomitans, p. gingivalis, p. intermedia/p. nigrescens group or c. rectus in their subgingival or salivary samples before treatment. the res ... | 1996 | 8811482 |
| occurrence of actinobacillus actinomycetemcomitans, porphyromonas gingivalis and prevotella intermedia in juvenile periodontitis. | the occurrence of actinobacillus actinomycetemcomitans, porphyromonas gingivalis and prevotella intermedia in subgingival plaque in 24 juvenile periodontitis patients was determined using dna probe. 36 samples of subgingival plaque from 36 pockets having > or = 6 mm depth, > or = 3 mm of loss of attachment, and bleeding on probing and/or suppuration were taken from 18 patients with localized juvenile periodontitis (ljp, age range 12-24 years); and 12 samples from 6 patients with generalized juve ... | 1996 | 8849845 |
| prevalence of 6 putative periodontal pathogens in subgingival plaque samples from romanian adult periodontitis patients. | the aim of the present study was to determine by standard cultivation procedures the detection frequencies of porphyromonas gingivalis, prevotella intermedia, fusobacterium nucleatum, actinobacillus actinomycetemcomitans, bacteroides forsythus, and capnocytophaga species as well as various enteric rods in subgingival plaque samples form romanian adult periodontitis patients. dna probe analysis (affirm dp microbial identification test) was also used, parallel to cultivation, to identify p. gingiv ... | 1996 | 8849850 |
| growth inhibition of a human oral bacterium porphyromonas gingivalis by rat cysteine proteinase inhibitor cystatin s. | agar diffusion analysis demonstrated that rat cystatin s, a cysteine proteinase inhibitor, inhibited the growth of all tested strains of a human oral, gram-negative anaerobic periodontopathogen porphyromonas gingivalis. its specific inhibitory activity against this tissue-invasive bacterium but not against other tested oral bacterial species emphasized the importance of specific cysteine proteinases for growth of p. gingivalis. | 1996 | 8862019 |
| osseointegrated implants in patients treated for generalized severe adult periodontitis. an interim report. | this study presents oral rehabilitation with osseointegrated implants in partially edentulous patients treated for generalized severe adult periodontitis. five female patients aged between 31 and 44 received a total of 36 implants and were observed for 1 year after insertion of the superstructure. three months before implantation, venous blood samples were taken from the patients and five periodontally healthy controls, and the serum examined with highly sensitive elisa test kits for interleukin ... | 1996 | 8866317 |
| an investigation into the use of sds-page of cell surface extracts and proteolytic activity to differentiate prevotella nigrescens and prevotella intermedia. | by comparison of the cell surface proteins derived from the outer membrane and fibrils from 14 prevotella intermedia and 19 prevotella nigrescens strains using sds and analysed by sds-page, it was possible to distinguish the two species. a polypeptide of approx. 21 kda distinguished p. intermedia strains, whereas two polypeptides of approx. 18 and 22 kda could be used to identify p. nigrescens strains. four other human oral black pigmented bacterial species (porphyromonas gingivalis, prevotella ... | 1996 | 8869494 |
| evaluation of the long-term efficacy and safety of locally-applied minocycline in adult periodontitis patients. | the objectives of the present study were to establish in a long-term investigation the safety as well as the clinical and microbiological efficacy of scaling and rootplaning combined with local application of 2% minocycline hydrochloride-gel versus placebo-gel in patients with moderate to severe chronic adult periodontitis. this was an 18 months, randomized, double-blind, parallel, comparative study, in which 20 healthy patients with moderate to severe chronic periodontitis participated. at base ... | 1996 | 8877655 |
| the relationship of gingival crevicular fluid short chain carboxylic acid concentration to gingival inflammation. | short-chain carboxylic acids (scca; c < or = 5; e.g., lactic acid, propionic acid, butyric acid) are metabolic by-products of bacterial metabolism which accumulate in the gingival crevice, and exhibit significant biological activity, including the ability to alter gene expression. it has been hypothesized that among the activities of sccas are their ability to contribute to gingival inflammation. this concept complements the notion that specific periodontal pathogens are the causative agents of ... | 1996 | 8877660 |
| clonality of porphyromonas gingivalis, prevotella intermedia and prevotella nigrescens isolated from periodontally diseased and healthy sites. | black-pigmented anaerobes have been implicated as major pathogens in the aetiology of adult periodontitis but these organisms are also found in healthy sites. this study aimed to examine the relationship between genotypes of black-pigmented anaerobes and disease status of periodontal sites using restriction endonuclease analysis (rea) and ribotyping. the main black-pigmented species recovered from sites were porphyromonas gingivalis, prevotella intermedia and prevotella nigrescens. each of the 5 ... | 1996 | 8884636 |
| treatment of periodontal disease based on microbiological diagnosis. a 5-year follow-up on individual patterns. | sixteen patients with advanced periodontitis (more than 3 sites with a probing depth > or = 6 mm) were treated with the aim of eliminating actinobacillus actinomyce-temcomitans and porphyromonas gingivalis and reducing prevotella intermedia below 5% of the total viable count. the patients were followed clinically and microbiologically for over 5 years and showed a highly individual pattern with respect to presence of indicator bacteria, type of treatment needed to accomplish treatment goals, cli ... | 1996 | 8884645 |
| compounds from syzygium aromaticum possessing growth inhibitory activity against oral pathogens. | a crude meoh extract of syzygium aromaticum (clove) exhibited preferential growth-inhibitory activity against gram-negative anaerobic periodontal oral pathogens, including porphyromonas gingivalis and prevotella intermedia. by means of bioassay-directed chromatographic fractionation, eight active compounds were isolated from this extract and were identified as 5,7-dihydroxy-2-methylchromone 8-c-beta-d-glucopyranoside, biflorin, kaempferol, rhamnocitrin, myricetin, gallic acid, ellagic acid, and ... | 1996 | 8904847 |
| pcr reaction and dot-blot hybridization to monitor the distribution of oral pathogens within plaque samples of periodontally healthy individuals. | the purpose of this study was to determine the distribution of the putative periodontal pathogens prevotella intermedia, prevotella nigrescens, the three oral capnocytophaga species (c. ochracea, c. sputigena, c. gingivalis), as well as porphyromonas gingivalis and actinobacillus actinomycetemcomitans in plaque samples of periodontally healthy individuals. we chose a newly developed 16s rdna directed polymerase chain reaction (pcr) and a previously described dot-blot hybridization assay to detec ... | 1996 | 8910839 |
| topographic distribution of black-pigmenting anaerobes before and after periodontal treatment by local delivery of tetracycline. | the purpose of this study was to determine the distribution of black-pigmenting gram-negative bacteria in the dentition of 10 adult periodontitis patients before and after treatment by local delivery of tetracycline. the subjects were selected based on a screening for high counts of porphyromonas gingivalis and multiple deep pockets. subgingival microbial samples were taken from the mesial and distal aspect of every tooth (44 to 56 sites per patient) before treatment, and after one and two month ... | 1996 | 8915018 |
| discrepancy between culture and dna probe analysis for the detection of periodontal bacteria. | the purpose of this study was to compare a commercially available dna probe technique with conventional cultural techniques for the detection of actinobacillus actinomycetemcomitans, porphyromonas gingivalis and prevotella intermedia in subgingival plaque samples. samples from 20 patients with moderate to severe periodontitis were evaluated at baseline and during a 15 months period of periodontal treatment. paperpoints from 4 periodontal pockets per patient were forwarded to omnigene for dna pro ... | 1996 | 8915026 |
| selling biotechnology in the dental medicine marketplace: the omnigene diagnostics dna probe tests for periodontal pathogens. | omnigene diagnostics, inc. has applied the principles of genetic engineering to develop species-specific dna probe tests for eight periodontal pathogens (porphyromonas gingivalis, prevotella intermedia, actinobacillus actinomycetem-comitans, fusobacterium nucleatum, eikenella corrodens, campylobacter rectus, bacteroides forsythus, and treponema denticola). the test requires minimal effort on the part of the clinician: subgingival plaque samples are collected from the patient and sent through the ... | 1996 | 8931245 |
| degradation of host protease inhibitors and activation of plasminogen by proteolytic enzymes from porphyromonas gingivalis and treponema denticola. | bacterial proteases may participate in the pathogenesis of periodontal diseases through their action on host proteins. in the present study, the ability of selected periodontopathogens, as well as two proteases isolated from porphyromonas gingivalis and treponema denticola, to degrade host protease inhibitors was evaluated. the activation of human plasminogen by the two bacterial proteases was also investigated. proteolytic breakdown of host protease inhibitors (alpha-1-antitrypsin, antichymotry ... | 1996 | 8936322 |
| characteristics of systemic antibody responses of nonhuman primates following active immunization with porphyromonas gingivalis, prevotella intermedia and bacteroides fragilis. | periodontal disease is an infectious disease manifested by the progressive change of a healthy resident commensal microbiota to a pathogenic one characterized by a specific microbiota. thus, the prospect for the use of selected bacteria or their antigens as a vaccine to interfere with the microbial changes and resulting progression of periodontal tissue destruction has been proposed. as a first step in examining the use of bacterial antigens as immunogens in periodontitis, this study characteriz ... | 1996 | 8941758 |
| distribution and genetic analysis of oral prevotella intermedia and prevotella nigrescens. | a total of 344 prevotella intermedia and nigrescens group isolates from 59 subjects were identified by hybridization with nonradioactively labeled species-specific oligonucleotide probes. identification of 20 p. intermedia and 46 p. nigrescens isolates was confirmed by analyzing the electrophoretic mobilities of malate and glutamate dehydrogenase enzymes. a total of 111 isolates (32%) were identified as p. intermedia and 233 isolates (68%) as p. nigrescens. identification performed with oligonuc ... | 1996 | 8941760 |
| characterization of prevotella intermedia and prevotella nigrescens by enzyme production, restriction endonuclease and ribosomal rna gene restriction analyses. | restriction endonuclease analysis, rrna gene restriction analysis (ribotyping), multilocus enzyme electrophoresis and lipase production were investigated for their potential to differentiate isolates belonging to the closely-related species prevotella intermedia and prevotella nigrescens. of 122 strains identified originally as p. intermedia, 52 were assigned to p. intermedia and 68 to p. nigrescens using multilocus enzyme electrophoresis. all 39 p. intermedia and 52 out of 53 p. nigrescens test ... | 1996 | 8941766 |
| risk indicators for periodontal disease in a racially diverse urban population. | a cross-sectional study of 117 subjects from a dental clinic serving a diverse population (i.e., whites, african-americans, native-americans, and asians) was performed to evaluate risk indicators of periodontal disease. gingival crevicular fluid (gcf) and subgingival plaque were taken at the same visit from 4 posterior sites of the most diseased sextant in each subject. age, smoking packyears, beta-glucuronidase (beta g), neutrophil elastase (ne), myeloperoxidase (mpo), fusobacterium nucleatum ( ... | 1996 | 8951624 |
| longitudinal evaluation of the development of periodontal destruction in spouses. | the aim of the present investigation was to study longitudinally the periodontal condition of married couples. the data are derived from a longitudinal study in a young population living in a remote village in indonesia and showing a relatively high prevalence of periodontal destruction. in 23 married couples, clinical measurements were carried out in 1987 and 1994. during the latter examination, a pooled gingival sample was obtained for microbiological evaluation. in 1994, the mean age of the g ... | 1996 | 8951630 |
| microbiological response to mechanical treatment in combination with adjunctive therapy. a review of the literature. | the recognition of the microbial origin and the specificity of periodontal infections has resulted in the development of several adjunctive therapies (antibiotics and/or antiseptics) to scaling and root planing in the treatment of chronic adult periodontitis. this article aims to review the "additional" effect of a subgingival irrigation with chlorhexidine, or a local or systemic application of tetracycline or metronidazole, performed in combination with a single course of scaling and root plani ... | 1996 | 8959563 |
| the adherence of periodontopathogens to periodontal probes. a possible factor in intra-oral transmission? | periodontal probes have previously been shown to harbor several bacterial types or species after probing periodontally diseased pockets. this study aims to identify and quantify periodontopathogens that may adhere to a periodontal probe by culturing techniques. it also examines the probe's roughness on its capability to collect bacteria, comparing merrit-b probes (with deep indentations) with tps probes (with smooth surfaces). from the differential phase contrast microscopy findings it was seen ... | 1996 | 8959565 |
| degradation of lactoferrin by periodontitis-associated bacteria. | the degradation of human lactoferrin by putative periodontopathogenic bacteria was examined. fragments of lactoferrin were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, and measured by densitometry. the degradation of lactoferrin was more extensive by porphyromonas gingivalis and capnocytophaga sputigena, slow by capnocytophaga ochracea, actinobacillus actinomycetemcomitans and prevotella intermedia, and very slow or absent by prevotella nigrescens, campylobacter rectus ... | 1996 | 8961558 |
| extracts of periodontopathic microorganisms lack functional superantigenic activity for murine t cells. | products of periodontopathic bacteria exert immunomodulatory effects on various lymphoid cell populations, some of which have been implicated in the pathogenesis of periodontitis. it has recently been suggested that some of these bacterial products may possess superantigenic (sag) activity. sag bind simultaneously to the v beta chain of t cell receptors and to class ii major histocompatibility complex molecules, thereby activating as many as 35% of t cells to proliferate and produce cytokines. i ... | 1996 | 8971649 |
| immune response to anaerobic bacteria in patients with peritonsillar cellulitis and abscess. | the role of four oral organisms (fusobacterium nucleatum. prevotella intermedia, porphyromonas gingivalis, and actinobacillus actinomycetemcomitans) was investigated in 19 children with peritonsillar abscess, and 17 with peritonsillar cellulitis. antibody titers to these organisms were measured by enzyme- linked immunosorbent assay in the patient, as well as in 32 control patients. serum levels in the patients were determined at day 1 and 42-56 days later. significantly higher antibody levels to ... | 1996 | 8973727 |
| polymerase chain reaction detection of 8 putative periodontal pathogens in subgingival plaque of gingivitis and advanced periodontitis lesions. | a 16s rrna-based polymerase chain reaction (pcr) detection method was used to determine the prevalence of actinobacillus actinomycetemcomitans, bacteroides forsythus, campylobacter rectus, eikenella corrodens, porphyromonas gingivalis, prevotella intermedia. prevotella nigrescens and treponema denticola in subgingival specimens of 50 advanced periodontitis, 50 adult gingivitis and 50 pediatric gingivitis subjects. the optimal pcr conditions were determined for each study species. agarose gel ele ... | 1996 | 9002880 |
| plaque ph and microflora of dental plaque on sound and carious root surfaces. | given the hypothesis that root caries is the result of acid formation by acidogenic micro-organisms, the present study was performed to relate sucrose-induced ph response of dental plaque on root surfaces to the microbial composition of the overlying plaque. seventeen caries-active elderly chinese with poor oral hygiene and with both sound and carious root surfaces were examined. plaque ph was measured before and up to one hour after a controlled sucrose mouthrinse. plaque samples for microbiolo ... | 1996 | 9003238 |
| detection and comparison of specific hemin binding by porphyromonas gingivalis and prevotella intermedia. | a radioligand assay was designed to detect and compare specific hemin binding by the periodontal anaerobic black-pigmenting bacteria (bpb) porphyromonas gingivalis and prevotella intermedia. the assay included physiological concentrations of the hemin-binding protein rabbit serum albumin (rsa) to prevent self-aggregation and nonspecific interaction of hemin with cellular components. under these conditions, heme-starved p. intermedia cells (two strains) expressed a single binding site species (4, ... | 1997 | 9006012 |
| electrophoresis of whole-cell soluble proteins of microorganisms isolated from bacteremias in endodontic therapy. | we have previously demonstrated that anaerobic bacteria are the microorganisms most frequently isolated from blood following endodontic therapy of teeth with apical periodontitis. phenotypic characterisation of the isolates suggested that the bacteria in the blood originated from the root canal. the present experiment using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (sds-page) was carried out in an effort to verify these findings, and to further study the microorganisms involved i ... | 1996 | 9021323 |
| anaerobic microorganisms in root canals of human teeth with chronic apical periodontitis detected by indirect immunofluorescence. | aiming to assess the presence of selected anaerobic microorganisms in root canals of human teeth with chronic apical periodontitis. 25 central and lateral upper incisors presenting with radiographic evidence of chronic apical periodontitis were studied. the pulp chamber was opened under aseptic conditions and samples of the root canal content were collected with sterile absorbent paper points, which were placed and dispersed in test tubes containing reduced transport medium rtt. aliquots were dr ... | 1996 | 9028199 |
| tetracycline resistance in prevotella isolates from periodontally diseased patients is due to the tet(q) gene. | tetracycline-resistance in gram-negative periodontal bacteria is often due to the presence of the tet(q) gene. in the present study the polymerase chain reaction (pcr) was used to examine 54 isolates of gram-negative anaerobic rods (prevotella intermedia, prevotella nigrescens and related or bacteroides-like species) for the presence of the tet(q) gene. the isolates were recovered from 42 patients with periodontal disease living in northern europe and north america. an 814 base-pair segment of t ... | 1996 | 9028255 |
| alveolar bone loss in rats infected with a strain of prevotella intermedia and fusobacterium nucleatum isolated from a child with prepubertal periodontitis. | prevotella intermedia and fusobacterium nucleatum are associated with various forms of periodontal disease. the purpose of the present study was to infect the clinical isolates of these periodontopathic bacteria and to induce a significant loss of alveolar bone in specific pathogen-free (spf) rats in the absence of ligatures. p. intermedia ykd8 and f. nucleatum ykz5 were isolated from a prepubertal periodontitis patient, while p. gingivalis mwb13 was from a patient with juvenile periodontitis. a ... | 1997 | 9029446 |
| [the significance of endocrine factors and microorganisms in the development of gingivitis in pregnant women]. | 40-100% of pregnant women suffer from the co-called pregnancy gingivitis. the cause of pregnancy gingivitis is possible multicausal: increased plasma female sex-hormones, alteration in dental plague and perhaps prevotella intermedia in the subgingival plague, together with alteration of immunoresponse. increasing levels of progesterone in the gingiva as well as estrogens due to specific receptors affect vascular permeability and exudation, provoke stasis of microcirculation, increase prostagland ... | 1996 | 9036576 |
| rapid diagnosis of periodontal infections: findings in aids patients. | a small number of bacterial pathogens in the human oral cavity cause the different forms of periodontal disease. of the approximately two hundred different oral bacterial species, about a dozen have been associated with these diseases including localized juvenile periodontitis, rapidly progressing periodontitis, and adult periodontitis. these species include actinobacillus actinomycetemcomitans, bacteroides forsythus, campylobacter rectus, porphyromonas gingivalis, and prevotella intermedia. sev ... | 1997 | 9037612 |
| short-chain carboxylic acid concentration in human gingival crevicular fluid. | short-chain carboxylic acids (e.g., lactic acid, propionic acid, butyric acid) are metabolic by-products of bacterial metabolism which can accumulate in the gingival crevice. it is of no small consequence, therefore, that 1- to 5-mm concentrations of these acids exhibit significant biological activity, including the ability to alter cell proliferation and gene expression in cells of importance to the periodontium. this communication reports on the in vivo concentrations of propionic and butyric ... | 1997 | 9042080 |
| microbial findings of deep peri-implant bone defects. | the objective of this study was to examine inflammatory tissue in deep peri-implant bone pockets (> 5 mm) for anaerobic bacteria colonization. the peri-implant inflammatory tissue of bone defects from 12 edentulous patients with 18 unsuccessful implants (imz type) was removed after surgical opening of the defects. after grinding the tissue with glass beads in nutrient solution, an aliquot of the suspension was plated and incubated on appropriate culture media. the quantitative and qualitative di ... | 1997 | 9048462 |
| the detection of actinobacillus actinomycetemcomitans, porphyromonas gingivalis and prevotella intermedia using an elisa in an adolescent population with early periodontitis. | the aim of the study was to compare the occurrence and levels of a. actinomycetemcomitans, p. gingivalis, and p. intermedia in the subgingival plaque from sites with and without early periodontitis in adolescents using an elisa. 47, 15- to 16-year-old adolescents (39 indo-pakistani, 8 white caucasian) were examined for clinical attachment level, probing depth, supragingival plaque, subgingival calculus and bleeding on probing on the mesio-buccal and disto-buccal aspects of the 1st molars and the ... | 1997 | 9049799 |
| prevalence of porphyromonas and prevotella species in the dental plaque of dogs. | the prevalence and populations of porphyromonas and prevotella species were determined in three samples of dental plaque from each of 34 healthy dogs. porphyromonas gingivalis was present in 68 per cent of the dogs and 47 per cent of the plaque samples. the counts of p gingivalis increased with age (p < 0.1), the amount of plaque (p < 0.05) and the degree of gingivitis (p < 0.1). prevotella intermedia was present in 44 per cent of the dogs and 23 per cent of the plaque samples. the counts of p i ... | 1997 | 9050176 |
| bacterial adherence to guided tissue regeneration barrier membranes exposed to the oral environment. | microbial colonization of barrier materials used in guided tissue regeneration (gtr) is known to adversely affect treatment outcomes. the purpose of this study was to compare the rate at which 11 commonly-occurring oral bacteria species colonize three different barrier materials (collagen, expanded polytetrafluoroethylene, and polylactic acid). the study group consisted of 10 systemically healthy individuals with no history of periodontal disease and absence of antimicrobial therapy within the p ... | 1997 | 9058336 |
| aerobic and anaerobic microbiology of bacterial tracheitis in children. | establish the aerobic and anaerobic microbiology of bacterial tracheitis in children. | 1997 | 9061728 |
| longitudinal study of prognostic factors in established periodontitis patients. | numerous indicators for disease progression have been described in the last decade. the purpose of this study was to examine, longitudinally, a large battery of clinical, microbiological, and immunological indicators, to try to determine whether the presence of one or a combination of these parameters at baseline, would correlate positively with increased attachment and or bone loss (true prognostic factors). following initial screening, 79 patients with established periodontitis were monitored ... | 1997 | 9062856 |
| prevalence of suspected periodontal pathogens identified using elisa in adolescents of differing ethnic origins. | the aim of this study was to determine the prevalence of actinobacillus actinomycetemcomitans, porphyromonas gingivalis and prevotella intermedia in a group of adolescents and investigate the association of these organisms with various clinical parameters. a total of 527, 11-13-year-old children, of whom 333 (63%) were white caucasian, 187 (35%) indo-pakistani and 7 (1%) afro-caribbean, participated in the study. subgingival plaque samples, collected from the mesio-buccal of both upper first per ... | 1997 | 9083896 |
| a clinical and microbiological evaluation of systemic and local metronidazole delivery in adult periodontitis patients. | the present study describes results on selected clinical and microbiological parameters obtained by treatment with local (elyzol) and systemic (flagyl) use of metronidazole alone and/or mechanical subgingival debridement in adult periodontitis. patients were randomly divided into local and systemic treatment groups each comprising 5 individuals in each of whom 4 sites (one site/ quadrant) with a probing depth of > or = 5 mm were selected and treated with separate treatment modalities. the overal ... | 1997 | 9083899 |
| periodontal bacteria colonizing oral mucous membranes in edentulous patients wearing dental implants. | the purpose of this study was to investigate the prevalence of the microbiota on the oral mucosal surfaces and in the peri-implant pocket in edentulous subjects with a past history of periodontitis. twenty edentulous subjects wearing dental implants for at least one year and with a history of periodontitis participated in this study. clinical parameters were assessed (plaque, redness, swelling, peri-implant probing depth [pipd], and bleeding on probing) and microbiological samples were taken (or ... | 1997 | 9100195 |
| periodontal diseases: microbial factors. | | 1996 | 9118283 |
| hen egg-white lysozyme inhibits biological activities of lipopolysaccharides from periodontopathic bacteria. | lysozyme has a bactericidal activity for some strains of gram-positive bacteria, by enzymatic cleavage of peptidoglycans that constitute the cell wall. hen egg-white lysozyme (hel) was tested in vitro for effects on biological activities of lipopolysaccharides from periodontopathic gram-negative bacteria. actinobacillus actinomycetemcomitans, prevotella intermedia and porphyromonas gingivalis. hel inhibited a wide range of activities of these lipopolysaccharides, including activation of limulus ... | 1997 | 9138195 |
| development of resistance to metronidazole and minocycline in vitro. | by local delivery of antibiotics to periodontal pockets, very high initial concentrations are often quickly succeeded by subinhibitory concentrations, which may facilitate development of bacterial resistance. the purpose of the present study was to investigate possible development of resistance in suspected periodontal pathogens after exposure to subinhibitory concentrations of metronidazole and minocycline. the minimal inhibitory concentration (mic) of 18 reference strains and 12 clinical isola ... | 1997 | 9144048 |
| clinical and microbiological effects of subgingival antimicrobial irrigation with citric acid as evaluated by an enzyme immunoassay and culture analysis. | the purpose of the present study was to compare an enzyme immunoassay with culture samples from untreated and non-surgically treated periodontal pockets and to assess the clinical and microbiological effects of citric acid irrigation as a supplement to scaling and root planing. the enzyme immunoassay used in this study is a chairside diagnostic tool aimed at identifying the presence of p. gingivalis, p. intermedia, and a. actinomycetemcomitans. six sites with pocket depths > or = 6 mm in each of ... | 1997 | 9150039 |
| microbiology of ligature-induced marginal inflammation around osseointegrated implants and ankylosed teeth in cynomolgus monkeys (macaca fascicularis). | the microbiota associated with ligature-induced marginal inflammation around osseointegrated dental implants, ankylosed teeth, and normal control teeth was investigated in 8 cynomolgus monkeys (macaca fascicularis). submucosal/subgingival plaque was sampled with paper points on the day of literature placement and after 7 weeks. the samples were evaluated by phase-contrast microscopy and by cultivation on enriched non-selective and various solid media. the submucosal/subgingival flora was changed ... | 1996 | 9151583 |
| the influence of abutment surface roughness on plaque accumulation and peri-implant mucositis. | bacterial adhesion to intra-oral, hard surfaces is firmly influenced by the surface roughness to these structures. previous studies showed a remarkable higher subgingival bacterial load on rough surfaces when compared to smooth sites. more recently, the additional effect of a further smoothening of intra-oral hard surfaces on clinical and microbiological parameters was examined in a short-term experiment. the results indicated that a reduction in surface roughness below r(a) = 0.2 microns, the s ... | 1996 | 9151584 |
| the influence of periodontitis on the subgingival flora around implants in partially edentulous patients. | the hypothesis that teeth act as reservoirs of micro-organisms for the colonization of oral implants has recently been stated several times. the present study aimed at examining, in partially edentulous patients with severe periodontitis, whether pockets around teeth and implants harbored a comparable micro-flora. in 6 patients (3 with refractory periodontitis and 3 with advanced chronic adult periodontitis), plaque samples were taken from a deep and shallow pocket around both teeth and implants ... | 1996 | 9151609 |
| possible lethal enhancement of toxins from putative periodontopathogens by nicotine: implications for periodontal disease. | to test the hypothesis that lethal synergy in the chick embryo model may occur between nicotine and bacterial products (cell-free extracellular toxins and cell lysates) of five putative periodontopathogens. | 1997 | 9155677 |
| the effects of smoking on periodontal structures: a literature review. | periodontal disease seems to be more prevalent in smokers than in nonsmokers. studies have reported both increases and decreases in gingival blood flow due to smoking. smoking does not increase the presence of the periodontopathogens actinobacillus actinomycetemcomitans, porphyromonas gingivalis and bacteroides intermedius. both the chemotaxis and the phagocytic capacity of the polymorphonuclear leukocytes (pmns) harvested from smokers are lower than with those harvested from nonsmokers. further ... | 1997 | 9170751 |