| [maggot therapy with lucilia sericata for debridement of chronic wounds]. | | 2008 | 18929922 |
| [genital myiasis in a cat]. | genital myiasis was diagnosed in a cat which was brought to the clinic for therapy six days after giving birth. ten dead larvae were collected from its genital organs. during the microscopic examination, the larvae were identified as the third stage larva of lucilia sericata. | 2008 | 18985579 |
| effect of the extract from salivary glands of lucilia sericata on human neutrophils. | there is incomplete information about host immune response to maggot therapy, nowadays increasingly used to clean chronic wounds from necrotic debris and infection. maggots are applied to the wound during the inflammatory phase. at the same time neutrophils infiltrate the inflammatory site as the first defense line of the organism. myeloperoxidase (mpo) and reactive oxygen species, generated during the respiratory burst by neutrophils, are the key players participating in microbial killing as we ... | 2008 | 18987603 |
| flies in the ointment: a morphological and molecular comparison of lucilia cuprina and lucilia sericata (diptera: calliphoridae) in south africa. | complementary nuclear (28s rrna) and mitochondrial (coi) genes were sequenced from blowflies that phenotypically resembled lucilia cuprina (w.), lucilia sericata (meigen) or exhibited characters of both species. the aim was to test a long-held hypothesis that these species hybridize under natural conditions in south africa (ullyett, 1945). blowflies were obtained predominantly from the cape town metropolitan area, but reference samples were acquired for l. sericata from pretoria. several l. cupr ... | 2009 | 19019139 |
| competitive interaction between larvae of lucilia sericata (meigen) and chrysomya albiceps (wiedemann) (diptera: calliphoridae). | rearing of l. sericata and c. albiceps in pure cultures of a variety of densities, at four constant temperature regimes, demonstrated an inverse relationship between egg density and the total development (f(2,6) = 264.35 and f(2,6) = 71.87 for l. sericata and c. albiceps, respectively p < 0.05), survivorship (f(2,9) = 68.41; p < 0.001 forl. sericata and f(2,9) = 12.35; p < 0.05 for c. albiceps) and adult size (for l. sericata: males f(2,9) = 58.94 and females f(2,9) = 140.09. for c. albiceps: ma ... | 2007 | 19070043 |
| septic injury-inducible genes in medicinal maggots of the green blow fly lucilia sericata. | lucilia sericata maggots are used world-wide in biosurgery for the medical treatment of nonhealing wounds because they ingest necrotic tissues and significantly promote healing. to gain further insight into interdependencies between ecological adaptation and molecular evolution of innate immunity in diptera, we used the suppression subtractive hybridization method to screen for genes that are differentially expressed in response to septic wounding of sterile second instar larvae of l. sericata. ... | 2009 | 19076250 |
| sheep blowfly strike risk and management in great britain: a survey of current practice. | the methods used for the control of sheep blowfly strike (ovine cutaneous myiasis) and the farm management factors associated with strike prevalence were examined using data from questionnaire survey returns provided by 966 sheep farmers in great britain, based on the period between march 2003 and february 2004. overall, 91% of participants treated prophylactically with insecticides against blowfly strike; 39% treated twice and 11% treated more than three times in the year. insect growth regulat ... | 2008 | 19120956 |
| myiasis in domestic animals in israel. | dipteran larvae were collected from wounds of 78 domestic animals. the larvae were identified as follows: wohlfahrtia magnifica, taken from dogs (the majority), goats, sheep, camels, horses and one cat; lucilia sericata, from cats, sheep, one horse, and one dog together with chrysomya albiceps; przhevalskiana silenus from goats and musca domestica from one sheep. the animals were treated with antibiotics, anti-inflammatory agents and insecticides and most animals fully recovered. we believe this ... | 2009 | 19251365 |
| practical applications of molecular biological species identification of forensically important flies. | species identification of the early colonizers on a corpse, such as calliphoridae and sarcophagidae, provides useful information for estimating the postmortem interval (pmi). however, the morphology of adult flies and nymphs is very similar among species of the same genus, and for many forensic scientists accurate identification is very difficult within their routine work. we present a simple and time-saving method for identification of forensically important flies by comparing sequenced 304 bp ... | 2009 | 19264529 |
| maggot debridement therapy with lucilia cuprina: a comparison with conventional debridement in diabetic foot ulcers. | this is prospective case-control study of more than 18 months performed to assess the effectiveness of maggot debridement therapy (mdt) with the sterile larvae of lucilia cuprina (a tropical blowfly maggot) for the treatment of diabetic foot ulcers. literature thus far has only reported results with the temperate maggot, lucilia sericata. this study documents outcome in diabetic foot wounds treated with maggot debridement versus those treated by conventional debridement alone. in this series of ... | 2009 | 19291114 |
| do maggots have an influence on bacterial growth? a study on the susceptibility of strains of six different bacterial species to maggots of lucilia sericata and their excretions/secretions. | the maggots of lucilia sericata are successfully used as a treatment for infected wounds. many articles are published about possible direct antibacterial properties of maggots and their excretions/secretions (es), but with different results. the present study reinvestigates the susceptibility of six bacterial strains to maggots and their es. | 2009 | 19362001 |
| [oral myiasis in a dog]. | oral myiasis was found in a dog that was brought to the pet clinic of veterinary faculty of harran university in bad condition and with a wound in its mouth. during microscopic examination, the larvae were identified as the first stage larvae of lucilia sericata. | 2009 | 19367556 |
| the insecticidal activity of four medicinal plants against the blowfly lucilia sericata (diptera: calliphoridae). | the larvae of lucilia sericata induce myiasis and transmit mycobacterial infections to humans and animals. consequently, the blowfly should be controlled for human welfare and economic reasons. | 2009 | 19416380 |
| sexual development in lucilia cuprina (diptera, calliphoridae) is controlled by the transformer gene. | insects use an amazing variety of genetic systems to control sexual development. a y-linked male determining gene (m) controls sex in the australian sheep blowfly lucilia cuprina, an important pest insect. in this study, we isolated the l. cuprina transformer (lctra) and transformer2 (lctra2) genes, which are potential targets of m. the lctra and lctra2 proteins are significantly more similar to homologs from tephritid insects than drosophila. the lctra transcript is alternatively spliced such t ... | 2009 | 19433631 |
| protophormia terraenovae. a new allergenic species in amateur fishermen of caceres, spain. | asticot maggot (blowfly, calliphoridae family) is the most important live bait used for angling in our country. prevalence of allergy to live fish bait in occupationally exposed workers has been described. the purpose of this study was to determine the prevalence of asticot allergy in amateur fishermen and the identification of marketed asticot species in cáceres, spain. | 2009 | 19445862 |
| examination of nocturnal blow fly (diptera: calliphoridae) oviposition on pig carcasses in mid-michigan. | the most common application of forensic entomology involves estimating a portion of the postmortem interval (pmi), which usually assumes that blow flies (diptera: calliphoridae) do not oviposit nocturnally. research objectives were to (1) investigate blow fly nocturnal oviposition in relation to sunrise and sunset in michigan; (2) evaluate abiotic variables postulated to affect blow fly oviposition; and (3) conduct laboratory experiments testing blow fly activity under complete darkness. in 2006 ... | 2009 | 19496441 |
| massive oculofacial myiasis infestation with lucilia sericata. | | 2009 | 19539868 |
| palpebro-orbital myiasis in a patient with basal cell carcinoma. | we report a patient with basal cell carcinoma presenting with severe myiasis in a large ulcer involving the upper and lower eyelid. | 2009 | 19551688 |
| maggot secretions suppress pro-inflammatory responses of human monocytes through elevation of cyclic amp. | maggots of the blowfly lucilia sericata are used for the treatment of chronic wounds. as monocytes may contribute to the excessive inflammatory responses in such wounds, this study focussed on the effects of maggot secretions on the pro-inflammatory activities of these cells. | 2009 | 19575178 |
| [description and key to the main species of calliphoridae (diptera) larvae of forensic importance from colombia]. | larvae of 13 blowfly species from colombia are described and an illustrated key for all them is presented. all larval instars of calliphora nigribasis macquart, cochliomyia macellaria (fabricius), chrysomya albiceps (wiedemann), chrysomya megacephala (fabricius), hemilucilia segmentaria (fabricius), hemilucilia semidiaphana (rondani), lucilia eximia (weidemann) are described, but the second and third instars of compsomyiops verena (walter), and only the third instar of calliphora vicina robineau ... | 2009 | 19618061 |
| amino acid derivatives from lucilia sericata excretions/secretions may contribute to the beneficial effects of maggot therapy via increased angiogenesis. | maggot therapy, utilizing the larvae of lucilia sericata, has been reported to reduce the bacterial load within wounds and also to enhance wound healing. maggot excretions/secretions (es) have been shown to have a role in the success of maggot therapy. while the protein content of es has been investigated, to date little research has focused on the small metabolites present in es and their potential contribution to the therapy. study of the molecular composition of the secretions and the potenti ... | 2010 | 19799603 |
| [maggot therapy in wound management]. | maggots' ability to prevent infections and promote wound healing has been known since the 19th century. increasing problems with treatment-resistant wounds and antibiotic-resistant bacteria has aroused interest in maggot therapy. | 2009 | 19844278 |
| new concepts and new weapons in implant infections. | molecular studies have cast light on new facts about the virulence factors of bacteria responsible for implant infections. recently, the biofilm matrix has been shown to include a variety of important structural components, including dna, in addition to polysaccharides and proteins. this finding has stimulated interest in substances able to disrupt biofilms by attacking both the poly-n-acetylglucosamine surface polysaccharide (pnag) and the extracellular dna (edna), namely, dispersin b, a pnag-d ... | 2009 | 19856268 |
| disruption of staphylococcus epidermidis biofilms by medicinal maggot lucilia sericata excretions/secretions. | chronic infections are commonly associated with biofilms formed by bacteria such as staphylococcus epidermidis. with the increase in antibiotic resistant bacteria, maggot debridement therapy has been reintroduced for the treatment of chronic wounds. studies have shown that the excretion/ secretions (es) of lucilia sericata larvae (maggots) contain many bioactive compounds which may contribute to the efficacy of maggot therapy. the present study evaluates the effect of l. sericata es on the forma ... | 2009 | 19856274 |
| molecular identification of forensically important blowfly species (diptera: calliphoridae) from germany. | forensic entomology applies knowledge about the behaviour and ecology of insects associated to corpses to homicide investigations. it is possible to calculate a minimum post-mortem interval by determining the age of the oldest blowfly larvae feeding on a corpse. the growth rate of the larvae is highly dependent on temperature and also varies between the different blowfly species infesting a corpse. it is, thus, crucial to correctly identify the species collected from a crime scene. to increase t ... | 2009 | 19862555 |
| wound myiasis in a patient with squamous cell carcinoma. | a 60-year-old, otherwise healthy, male farmer presented to our dermatology department with a large ulcer on his lower right leg. the lesion had started as a small papule 6 months before, which became eroded and transformed into a rather rapidly progressive ulcer. on careful inspection, numerous larvae were found moving within the wound. the larvae were analyzed and found to be lucilia sericata (the green bottle blowfly). the lesion was diagnosed histopathologically as squamous cell carcinoma. th ... | 2009 | 19882087 |
| effects of the essential oil constituent thymol and other neuroactive chemicals on flight motor activity and wing beat frequency in the blowfly phaenicia sericata. | the effects were evaluated of the plant terpenoid thymol and eight other neuroactive compounds on flight muscle impulses (fmis) and wing beat frequency (wbf) of tethered blowflies (phaenicia sericata meig.). | 2010 | 19890946 |
| quorum-sensing-regulated virulence factors in pseudomonas aeruginosa are toxic to lucilia sericata maggots. | maggot debridement therapy (mdt) is widely used for debridement of chronic infected wounds; however, for wounds harbouring specific bacteria limited effect or failure of the treatment has been described. here we studied the survival of lucilia sericata maggots encountering pseudomonas aeruginosa pao1 in a simple assay with emphasis on the quorum-sensing (qs)-regulated virulence. the maggots were challenged with gfp-tagged p. aeruginosa wild-type (wt) pao1 and a gfp-tagged p. aeruginosa deltalasr ... | 2010 | 19892758 |
| lucifensin, the long-sought antimicrobial factor of medicinal maggots of the blowfly lucilia sericata. | a novel homologue of insect defensin designated lucifensin (lucilia defensin) was purified from the extracts of various tissues (gut, salivary glands, fat body, haemolymph) of green bottle fly (lucilia sericata) larvae and from their excretions/secretions. the primary sequence of this peptide of 40 residues and three intramolecular disulfide bridges was determined by esi-qtof mass spectrometry and edman degradation and is very similar to that of sapecin and other dipteran defensins. we assume th ... | 2010 | 19921400 |
| a simple, heat-sterilizable artificial diet excluding animal-derived ingredients for adult blowfly, lucilia sericata. | artificial diets have been developed for lucilia sericata (meigen) blowfly larvae; however, diets for adults have not yet been developed. an adult diet that excludes animal tissues and animal-derived ingredients and promotes not only ovarian development, but also oviposition, would aid in basic research and maggot debridement therapy. we have successfully developed artificial diets that exclude animal tissues and animal-derived ingredients for l. sericata adults. the outcomes of the diets were c ... | 2009 | 19941610 |
| maggot secretions skew monocyte-macrophage differentiation away from a pro-inflammatory to a pro-angiogenic type. | maggots of the blowfly lucilia sericata are used for the treatment of chronic wounds. earlier we reported maggot secretions to inhibit pro-inflammatory responses of human monocytes. the aim of this study was to investigate the effect of maggot secretions on the differentiation of monocytes into pro-inflammatory (mø-1) and anti-inflammatory/pro-angiogenic macrophages (mø-2) as these cells play a central role in wound healing. | 2009 | 19956650 |
| a nasal myiasis in a 76-year-old female in korea. | on july 2009, 5 fly larvae were discovered inside the nose of a 76-year-old female. she was living in cheonan-si, and in a state of coma due to rupture of an aortic aneurysm. surgery was performed on the day of admission, and the larvae were found 4 days later. by observing their posterior spiracle, the larvae were identified as lucilia sericata. considering the rapid development of this species, the infection was likely acquired during hospitalization. further investigation on the hospital envi ... | 2009 | 19967091 |
| the population genetic structure of north american lucilia sericata (diptera: calliphoridae), and the utility of genetic assignment methods for reconstruction of postmortem corpse relocation. | lucilia sericata (diptera: calliphoridae) is a common urban blowfly, with a worldwide distribution. it is among the most important forensic insects, and a major veterinary pest. a previous amplified fragment length polymorphism (aflp) survey of phormia regina, another blowfly, found that the north american population structure results from the fact that flies trapped together at a bait are predominantly comprised of related individuals. we report here a similar population genetic pattern for l. ... | 2010 | 20005649 |
| how promptly do blowflies colonise fresh carcasses? a study comparing indoor with outdoor locations. | we investigated the time taken by blowflies to find and oviposit on fresh carcasses placed outdoors and indoors. paired dead piglets, one in the open and the other in a nearby room (on the first floor of an occupied, detached, suburban house near cologne, germany, with a window opened 9 cm) were exposed simultaneously on nine occasions. the species visiting both locations and the number of egg batches deposited by blowflies between both locations were monitored 2, 8, 24 and 48 h after exposure. ... | 2010 | 20044223 |
| [the treatment of suppurative chronic wounds with maggot debridement therapy]. | maggot debridement therapy (mdt) is the intentional treatment of suppurative skin infections with the larvae of the fly, lucilia sericata. today, this treatment modality is being used in over 30 countries and during the last 20 years, more than 60,000 patients have been treated in 2,000 medical institutions. sterile maggots, produced in university laboratories and by private industry, are usually applied to the wound either by using a cage-like dressing or a tea bag-like cage. during the therapy ... | 2009 | 20101584 |
| variation in developmental time for geographically distinct populations of the common green bottle fly, lucilia sericata (meigen). | time between death and discovery of remains, or postmortem interval (pmi), can be assessed using blow fly maggot age. forensic entomologists rely on published, often nonlocal, species-specific developmental tables to determine maggot age. in a series of common garden experiments, we investigated the developmental rate variation between populations of lucilia sericata collected from sacramento, ca, san diego, ca, and easton, ma at 16 degrees c, 26 degrees c, and 36 degrees c. for the 16 degrees c ... | 2010 | 20102471 |
| [how many neuroblasts build mushroom bodies in lucilia caesar l. and musca domestica l. (diptera, brachycera cyclorrhapha)?]. | the green-bottle fly lucilia caesar and the housefly musca domestica differ greatly in the number of neuroblasts producing mushroom bodies. four neuroblasts were found in each mushroom body of lucilia pupae, and its calyx has a quadruple structure. in the housefly, the number of mushroom body neuroblasts rises up 20 in each brain hemisphere. this leads to a more complicated calyx structure. the neuroblast number observed in lucilia and musca is compared with that found in other diptera. | 2009 | 20146414 |
| combinations of maggot excretions/secretions and antibiotics are effective against staphylococcus aureus biofilms and the bacteria derived therefrom. | maggots of the blowfly lucilia sericata are used for the treatment of chronic wounds. previously we reported that maggot excretions/secretions (es) break down staphylococcus aureus biofilms but do not kill the bacteria. as many antibiotics are not effective against biofilms we assessed the effect of combinations of es and antibiotics on s. aureus biofilms and on the survival of the bacteria released from the biofilms. | 2010 | 20189943 |
| endogenous boldenone-formation in cattle: alternative invertebrate organisms to elucidate the enzymatic pathway and the potential role of edible fungi on cattle's feed. | although beta-boldenone (bbol) used to be a marker of illegal steroid administration in calves, its endogenous formation has recently been demonstrated in these vertebrates. however, research on the pathway leading to bbol remains scarce. this study shows the usefulness of in vivo invertebrate models as alternatives to vertebrate animal experiments, using neomysis integer and lucilia sericata. in accordance with vertebrates, androstenedione (aed) was the main metabolite of beta-testosterone (bt) ... | 2010 | 20197090 |
| comparison of traps for the control of sheep blowfly in the u.k. | the ability of three commercially available trap types to catch lucilia (diptera: calliphoridae) blowflies was assessed on three sheep farms in southwest england in 2008. the aim was to evaluate their relative value for the control of ovine cutaneous myiasis (sheep blowfly strike) on farms. there was a highly significant difference between the total number of female lucilia caught per day by the traps, with an agrilure trap (agrimin ltd, brigg, u.k.) catching more than the other trap types (resc ... | 2010 | 20202108 |
| fatty acid extracts from lucilia sericata larvae promote murine cutaneous wound healing by angiogenic activity. | fatty acids are considered to be effective components to promote wound healing and lucilia sericata larvae are applied clinically to treat intractable wounds. we aimed to investigate the effect of fatty acid extracts from dried lucilia sericata larvae on murine cutaneous wound healing as well as angiogenesis. | 2010 | 20211009 |
| infestation of an owl (bubo bubo) with lucilia spp. | myiasis is an infestation of tissue with the larval stage of dipterous flies. this condition mostly affects the skin but may also occur in certain body cavities. it can occur in either animals or humans and is caused by parasitic dipterous fly larvae feeding on the host's necrotic or living tissue. this disease rarely effects birds especially owls. in this study, infestation of an owl with cutaneous myiasis is reported. in october 2008, a wounded owl was referred by the environmental department ... | 2010 | 20234830 |
| the control of the sheep blowfly with d.d.t. and benzene hexachloride (666). | | 1947 | 20258847 |
| toxicity and repellency of certain organic compounds to larvae of lucilia sericata. | | 1946 | 20278195 |
| indoors forensic entomology: colonization of human remains in closed environments by specific species of sarcosaprophagous flies. | fly species that are commonly recovered on human corpses concealed in houses or other dwellings are often dependent on human created environments and might have special features in their biology that allow them to colonize indoor cadavers. in this study we describe nine typical cases involving forensically relevant flies on human remains found indoors in southern finland. eggs, larvae and puparia were reared to adult stage and determined to species. of the five species found the most common were ... | 2010 | 20304573 |
| an assessment of the antibacterial activity in larval excretion/secretion of four species of insects recorded in association with corpses, using lucilia sericata meigen as the marker species. | the relative antibacterial activities of excretion/secretion (es) from two carrion-feeding insects, calliphora vicina robineau-desvoidy and dermestes maculatus degeer, and a detritivore, tenebrio molitor linnaeus, were compared to that of lucilia sericata meigen, a species with es of known antibacterial capacity, in order to explore the antimicrobial potential of other carrion and detritivore species. viable counts were used to assess time-kill of es against five bacterial species, staphylococcu ... | 2010 | 20307342 |
| determination of calliphoridae (diptera) fauna and seasonal distribution on carrion in ankara province. | this study was conducted from march 2006 to 2007. the aim of the study was to determine the forensically significant calliphoridae (diptera) species and their seasonal distribution in ankara province at beytepe campus. pig carcasses were killed by a veterinary using pentobarbital sodium with intervals from 2 to 3 weeks to a month. samples were collected from the carcass everyday in the morning, in the afternoon, and at sunset. temperature and parameters of weather, such as cloudy, sunny, rainy, ... | 2010 | 20345774 |
| temperature-dependent development of the parasitoid tachinaephagus zealandicus on five forensically important carrion fly species. | the influences of temperature and host species on the development of the forensically important parasitoid tachinaephagus zealandicus ashmead (hymenoptera: encyrtidae) were studied at six constant temperatures in the range of 15-30 degrees c. t. zealandicus completed development successfully between 15 degrees c and 27 degrees c on five species of calliphoridae, calliphora albifrontalis malloch, calliphora dubia macquart, lucilia sericata meigen, chrysomya rufifacies macquart and chrysomya megac ... | 2010 | 20374476 |
| arthropods of forensic importance on pig carrion in the coahuilan semidesert, mexico. | this is the first report of an ongoing research to establish a sarcosaprophagous arthropod database in the coahuilan semidesert. seven pigs (sus scrofa l.) were used as human models to determine succession in an open urban area during the 2007 winter-spring period. arthropods were collected manually and from pitfall traps. carcass biomass loss, as well as arthropod colonization, was recorded during 71 days postmortem. five decomposition stages were identified during which most abundant orders we ... | 2010 | 20384924 |
| a new simulation-based model for calculating post-mortem intervals using developmental data for lucilia sericata (dipt.: calliphoridae). | homicide investigations often depend on the determination of a minimum post-mortem interval (pmi(min)) by forensic entomologists. the age of the most developed insect larvae (mostly blow fly larvae) gives reasonably reliable information about the minimum time a person has been dead. methods such as isomegalen diagrams or adh calculations can have problems in their reliability, so we established in this study a new growth model to calculate the larval age of lucilia sericata (meigen, 1826). this ... | 2010 | 20440626 |
| degradation of eschar from venous leg ulcers using a recombinant chymotrypsin from lucilia sericata. | larvae of the greenbottle lucilia sericata are used to debride nonhealing wounds and stimulate the production of fresh granulation tissue. previous publications have shown that secretions from l. sericata contain a number of proteolytic activities including a chymotrypsin that degrades a number of extracellular matrix components such as fibronectin, laminin and collagen. | 2010 | 20491762 |
| decomposed flesh as a vitellogenic protein source for the forensically important lucilia sericata (diptera: calliphoridae). | for the carrion-feeding blow flies, a common source of vitellogenic protein are the carcasses that also serve as larval substrates. this attraction continues well after the blow flies have ceased to oviposit on the carrion, and it has been assumed that a decomposed cadaver remains a suitable source for vitellogenic protein; however, this assumption has never been tested. to test this hypothesis, blow flies (lucilia sericata meigen) were supplied with either fresh or decomposed beef liver as prot ... | 2010 | 20496598 |
| an accidental but safe and effective use of lucilia cuprina (diptera: calliphoridae) in maggot debridement therapy in alexandria, egypt. | the calliphorid fly, lucilia cuprina (wiedemann), is known to cause serious malign myiasis in animals, whereas its sibling species lucilia sericata (meigen) is commonly a carrion breeder and is used in maggot debridement therapy (mdt). the current study reports an accidental involvement of l. cuprina in mdt in alexandria, egypt, that has proved to be safe and effective. in november 2008, the laboratory colonies of l. sericata (the species regularly used in mdt) at the faculty of science, alexand ... | 2010 | 20496600 |
| the mitochondrial genome of the common cattle grub, hypoderma lineatum. | the mitochondrial dna of the cattle grub hypoderma lineatum (de villers) (diptera: oestridae) was completely sequenced. the entire molecule was 16,354 bp long and presented a heavy bias towards a + t, which accounted for 77.8% of the whole genome. hypoderma lineatum genes were organized in the same order and orientation as in the mitochondrial genomes available for other species belonging to the oestroidea superfamily and compared in this study [chrysomya putoria (wiedemann), cochliomyia hominiv ... | 2010 | 20497317 |
| a genomics-informed, snp association study reveals fbln1 and fabp4 as contributing to resistance to fleece rot in australian merino sheep. | fleece rot (fr) and body-strike of merino sheep by the sheep blowfly lucilia cuprina are major problems for the australian wool industry, causing significant losses as a result of increased management costs coupled with reduced wool productivity and quality. in addition to direct effects on fleece quality, fleece rot is a major predisposing factor to blowfly strike on the body of sheep. in order to investigate the genetic drivers of resistance to fleece rot, we constructed a combined ovine-bovin ... | 2010 | 20500888 |
| mtdna-based identification of lucilia cuprina (wiedemann) and lucilia sericata (meigen) (diptera: calliphoridae) in the continental united states. | existing data suggest that the forensically important dipteran species lucilia cuprina (wiedemann) and lucilia sericata (meigen) may be particularly difficult to discriminate using dna sequence data. l. cuprina is paraphyletic with respect to l. sericata in mtdna phylogenies, with some l. cuprina having mtdna haplotypes that are very similar to those of l. sericata. we examine this problem by providing the first dna data for l. cuprina from north america, including portions of both the mitochond ... | 2010 | 20537828 |
| a novel approach to the antimicrobial activity of maggot debridement therapy. | commercially produced sterile green bottle fly lucilia sericata maggots are successfully employed by practitioners worldwide to clean a multitude of chronic necrotic wounds and reduce wound bacterial burdens during maggot debridement therapy (mdt). secretions from the maggots exhibit antimicrobial activity along with other activities beneficial for wound healing. with the rise of multidrug-resistant bacteria, new approaches to identifying the active compounds responsible for the antimicrobial ac ... | 2010 | 20542901 |
| evaluation of the tissue toxicity of antiseptics by the hen's egg test on the chorioallantoic membrane (hetcam). | antiseptics are frequently used for the prophylaxis and treatment of local infections of chronic wounds. whereas local antiseptics in general have a positive effect on wound healing an uncritical use may impair wound healing due to toxic side effects. | 2010 | 20562059 |
| the antibacterial potency of the medicinal maggot, lucilia sericata (meigen): variation in laboratory evaluation. | research to quantify the potency of larval excretion/secretion from lucilia sericata using liquid culture assays has produced contradictory results. in this study, viable counting was used to investigate the effectiveness of excretion/secretion against three marker bacterial species (pseudomonas aeruginosa, staphylococcus aureus, and escherichia coli) and the effects of varying growing conditions in assays. results demonstrate that factors such as number of larvae, species of bacteria and additi ... | 2010 | 20600366 |
| evaluation of reference genes for real-time pcr quantification of gene expression in the australian sheep blowfly, lucilia cuprina. | the australian sheep blowfly, lucilia cuprina (wiedemann) (diptera: calliphoridae), is an important pest of sheep in australia and other parts of the world. however, the paucity of information on many fundamental molecular aspects of this species limits the ability to exploit functional genomics techniques for the discovery of new drug targets for its control. the present study aimed to facilitate gene expression studies in this species by identifying the most suitable reference genes for normal ... | 2010 | 20604863 |
| [sequencing of mitochondrial dna co ii gene in carrion-breeding flies and larvae in zhengzhou]. | using co ii sequences to identify common species of carrion-breeding flies and larvae. | 2010 | 20653135 |
| [biosurgical débridement using lucilia sericata-maggots - an update]. | during the last years an increasing number of patients suffering from therapy refractory chronic wounds which are frequently infected by multi-resistant bacteria - e.g. methicillin-resistant staphylococcus aureus - have led to an increasing interest in the treatment using larvae or maggots of the blow fly species lucilia sericata. maggots are responsible for necrectomy and they have antimicrobial activity in particular against grampositive bacteria like a disinfectant of the wound. it is conclud ... | 2010 | 20714816 |
| [sequencing of mitochondrial dna cytochrome oxidase subunit i gene in sarcosaphagous flies from 14 provinces in china]. | to detect the 278 bp region of gene of the cytochrome oxidase subunit i (coi) in mitochondral dna (mtdna) of sarcosaphagous flies, identify the species of sarcosaphagous flies, and provide reference for forensic application. | 2010 | 20818074 |
| quantification of methadone and its metabolite 2-ethylidene-1,5-dimethyl-3,3-diphenylpyrrolidine in third instar larvae of lucilia sericata (diptera: calliphoridae) using liquid chromatography-tandem mass spectrometry. | entomotoxicology studies the application of toxicological analysis on necrophageous insects present on human remains. this paper describes the development and validation of a sensitive liquid chromatography tandem mass spectrometry method for quantification of methadone and its main metabolite, 2-ethylidene-1,5-dimethyl-3,3-diphenylpyrrolidine (eddp), in developmental stages of lucilia sericata. one single larva was pulverized in a disposable vial and then extracted with 1-chlorobutane. after ev ... | 2010 | 20822674 |
| comparison of decomposition rates and faunal colonization of carrion in indoor and outdoor environments. | decomposition and insect colonization of pig (sus scrofa l.) carcasses were observed over a 42-day period inside and outside a house in a suburban region of edmonton, alberta. three freshly killed pig carcasses were placed outdoors on grass and three carcasses were placed in separate rooms inside a house in a suburban residential area. the carcasses were examined and sampled regularly. outside carcasses were colonized rapidly by calliphora vicina (r-d), lucilia sericata (meigen), lucilia illustr ... | 2011 | 20840295 |
| organisation and expression of a cluster of yolk protein genes in the australian sheep blowfly, lucilia cuprina. | the australian sheep blowfly lucilia cuprina is a major pest for the australian and new zealand sheep industries. with the long-term aim of making a strain of l. cuprina suitable for a genetic control program, we previously developed a tetracycline-repressible female lethal genetic system in drosophila. a key part of this system is a female-specific promoter from a yolk protein (yp) gene controlling expression of the tetracycline-dependent transactivator (tta). here we report the sequence of a 1 ... | 2011 | 20844939 |
| efficient germ-line transformation of the economically important pest species lucilia cuprina and lucilia sericata (diptera, calliphoridae). | the green blowfly species lucilia cuprina and lucilia sericata are economically important pests for the sheep industries of australia and new zealand. l. cuprina has long been considered a good target for a genetic pest management program. in addition, l. sericata maggots are used in the cleaning of wounds and necrotic tissue of patients suffering from ulcers that are difficult to treat by other methods. development of efficient transgenesis methods would greatly facilitate the development of st ... | 2010 | 20869440 |
| conservation and sex-specific splicing of the doublesex gene in the economically important pest species lucilia cuprina. | genetic control of sex determination in insects has been best characterized in drosophila melanogaster, where the master gene sxl codes for rna that is sex specifically spliced to produce a functional protein only in females. sxl regulates the sex-specific splicing of transformer (tra) rna which, in turn, regulates the splicing of dsx rna to produce functional male and female proteins. in the australian sheep blowfly (lucilia cuprina), the tra gene (lctra) is required for female development and ... | 2010 | 20876994 |
| induction of antibacterial activity in larvae of the blowfly lucilia sericata by an infected environment. | maggot debridement therapy (mdt) is a method for the treatment of intractable, infected and necrotic wounds. in mdt, sterile larvae of lucilia sericata meigen (diptera: calliphoridae) are applied to infected wounds, where they exert antibacterial effects. once the larvae are placed in the wound, they are no longer germ-free. this study analysed the influence of infected environments on larval antibacterial activities. sterile larvae were mixed in a test tube containing a bacterial suspension of ... | 2010 | 20946439 |
| [succession of sarcosaphagous insects at summer and autumn in shijiazhuang area]. | to study the succession of sarcosaphagous insects and their regular activity on carcass in shijiazhuang area. | 2010 | 20967949 |
| conjunctival myiasis; caused by lucilia sericata, green bottle fly. | | 1945 | 20997144 |
| spraying trials with d.d.t. and 666 against the sheep blowfly. | | 1945 | 21014203 |
| lucilia sericata strain from colombia: experimental colonization, life tables and evaluation of two artificial diets of the blowfly lucilia sericata (meigen) (diptera: calliphoridae), bogotá, colombia strain. | the objective of this work was to establish, under experimental laboratory conditions, a colony of lucilia sericata, bogotá-colombia strain, to build life tables and evaluate two artificial diets. this blowfly is frequently used in postmortem interval studies and in injury treatment. the parental adult insects collected in bogotá were maintained in cages at 22°c±1 average temperature, 60%±5 relative humidity and 12 h photoperiodicity. the blowflies were fed on two artificial diets that were eval ... | 2010 | 21031265 |
| alteration of expirated bloodstain patterns by calliphora vicina and lucilia sericata (diptera: calliphoridae) through ingestion and deposition of artifacts. | bloodstain pattern analysis can provide insight into a sequence of events associated with a violent crime. however, bloodstain pattern analysis can be confounded by the feeding activity of blow flies. we conducted two laboratory experiments to investigate the relationships between lucilia sericata (green bottle fly) and calliphora vicina (blue bottle fly), expirated bloodstains, and pooled bloodstains on a range of surfaces (linoleum, wallpaper, textured paint). c. vicina and l. sericata changed ... | 2010 | 21039518 |
| the ability of the blowflies calliphora vomitoria (linnaeus), calliphora vicina (rob-desvoidy) and lucilia sericata (meigen) (diptera: calliphoridae) and the muscid flies muscina stabulans (fallén) and muscina prolapsa (harris) (diptera: muscidae) to colonise buried remains. | the blowflies calliphora vomitoria (linnaeus), calliphora vicina (rob-desvoidy) and lucilia sericata (meigen) exhibited a limited ability to colonise pig liver baits buried in loose soil. calliphora vomitoria colonised baits buried at 5 cm but no deeper whilst c. vicina and l. sericata colonised remains at 10 cm but not at 20 cm. the baits were colonised by larvae hatching from eggs laid on the surface of the soil. both c. vomitoria and l. sericata were able to develop from eggs through to adult ... | 2010 | 21071161 |
| gene expression during blow fly development: improving the precision of age estimates in forensic entomology. | forensic entomologists use size and developmental stage to estimate blow fly age, and from those, a postmortem interval. since such estimates are generally accurate but often lack precision, particularly in the older developmental stages, alternative aging methods would be advantageous. presented here is a means of incorporating developmentally regulated gene expression levels into traditional stage and size data, with a goal of more precisely estimating developmental age of immature lucilia ser ... | 2010 | 21155802 |
| conserved function of the krüppel gap gene in the blowfly lucilia sericata, despite anterior shift of expression. | to determine whether expression patterns of segmentation genes found in drosophila melanogaster can be scaled to pattern larger insects, we studied the expression of the krüppel (kr) gene in the blowfly lucilia sericata. compared with drosophila kr, l. sericata kr showed an unexpected 10% shift of expression towards the anterior pole. furthermore, expression domains not found in d. melanogaster were present at the blastoderm stage of l. sericata. to compare kr activity and function, we employed ... | 2010 | 21166911 |
| maggot chymotrypsin i from lucilia sericata is resistant to endogenous wound protease inhibitors. | a chymotrypsin found in the secretions of lucilia sericata and manufactured as a recombinant enzyme degrades chronic wound eschar ex vivo. | 2010 | 21175562 |
| selective sweeps at the organophosphorus insecticide resistance locus, rop-1, have affected variation across and beyond the α-esterase gene cluster in the australian sheep blowfly, lucilia cuprina. | a major theoretical consequence of selection at a locus is the genetic hitchhiking of linked sites (selective sweep). the extent of hitchhiking around a gene is related to the strength of selection and the rate of recombination, with its impact diminishing with distance from the selected site. at the rop-1 locus of the sheep blowfly, lucilia cuprina, polymorphisms at two different sites within the lcαe7 gene encode forms of the protein that confer organophosphorus insecticide resistance. to asse ... | 2011 | 21228400 |
| control of the myiasis-producing fly, lucilia sericata, with egyptian essential oils. | myiasis caused by lucilia sericata (diptera: calliphoridae) is widely distributed throughout the world and affects both humans and animals. in addition, l. sericata larvae and adults may play a role in spreading causal agents of mycobacterial infections. therefore, it is important to establish new and safe alternative methods of controlling this blowfly. | 2011 | 21244384 |
| [comparison of mtdna extraction from different parts of sarcosaphagous insects]. | to explore mitochondrial dna (mtdna) extraction effects of different parts from sarcosaphagous insects using improved cetyltriethylammnonium bromide (ctab) method. | 2010 | 21287735 |
| methadone determination in puparia and its effect on the development of lucilia sericata (diptera, calliphoridae). | this paper describes a sensitive uplc-ms/ms method for quantification of methadone and 2-ethylidene-1,5-dimethyl-3,3-diphenylpyrrolidine (eddp) in single empty puparial case of lucilia sericata. larvae were reared on substrates spiked with different concentrations of methadone (0-4 µg/g). methadone was quantified in puparia reared on high concentrated substrates (0.8-4 µg/g). the major metabolite of methadone (eddp) was not detected, confirming rapid elimination of metabolites by the larvae befo ... | 2011 | 21320760 |
| genomic structure and sequence analysis of lucilia cuprina hsp90 gene. | the hsp90 family is one of the highly conserved chaperone families, varying between eubacteria to higher vertebrates. the hsp90 protein has been assigned different functions including thermal protection, but having major role in development. the present study is a detailed analysis of the structural characteristics of hsp90 gene (lchsp90) of sheep blowfly, lucilia cuprina. the gene isolated by pcr revealed absence of intron. the nucleic acid and amino acid comparison revealed significant level o ... | 2011 | 21366970 |
| effectiveness of wound cleansing treatments on maggot (diptera, calliphoridae) mortality. | myiasis is defined as an infestation of the organs and/or tissues of human and other animals by fly maggots. fly species that normally breed in meat or carrion (diptera: calliphoridae, sarcophagidae) may become involved in cutaneous myiasis by colonizing preexisting wounds. reports of human wound myiasis contracted in hospitals and nursing homes, especially when patients are chronically ill or bed-ridden, are not uncommon across north america and often result in cases of neglect and civil litiga ... | 2011 | 21377818 |
| modelling the impact of climate change on spatial patterns of disease risk: sheep blowfly strike by lucilia sericata in great britain. | understanding the spatial scale and temporal pattern of disease incidence is a fundamental prerequisite for the development of appropriate management and intervention strategies. it is particularly critical, given the need to understand the elevated risks linked to climate change, to allow the most likely changes in the distribution of parasites and disease vectors to be predicted under a range of climate change scenarios. using statistical models, the spatial distribution and climatic correlate ... | 2011 | 21397606 |
| recombinant lucilia sericata chymotrypsin in a topical hydrogel formulation degrades human wound eschar ex vivo. | larval biotherapy is a debridement tool used in wound management. the mechanism of action involves degradation of eschar by serine proteases including chymotrypsin within the alimentary fluids of first instar lucilia sericata. with the rationale of obviating some limitations of biotherapy, including cost, complexity of use, and patient reticence, the present study describes a mobile hydrogel formulation containing freeze-dried recombinant l. sericata chymotrypsin designed for topical application ... | 2011 | 21509949 |
| the characterization of lucilia cuprina acetylcholinesterase as a drug target, and the identification of novel inhibitors by high throughput screening. | acetylcholinesterase (ache, ec3.1.1.7.) is the molecular target for the carbamate and organophosphate pesticides that are used to combat parasitic arthropods. in this paper we report the functional heterologous expression of ache from lucilia cuprina (the sheep blowfly) in hek293 cells. we show that the expressed enzyme is cell-surface-exposed and possesses a glycosyl-phosphatidylinositol membrane anchor. the substrates acetyl-, propionyl- and butyrylthiocholine (actc, proptc, buttc), and also 1 ... | 2011 | 21530657 |
| changes in the morphology and presumptive chemistry of impact and pooled bloodstain patterns by lucilia sericata (meigen) (diptera: calliphoridae)* | bloodstain pattern analysis can be critical to accurate crime scene reconstruction. however, bloodstain patterns can be altered in the presence of insects and can confound crime scene reconstruction. to address this problem, we conducted a series of controlled laboratory experiments to investigate the effect of lucilia sericata (meigen) on impact bloodstains and pooled bloodstains in association with three combinations of common surfaces (linoleum/painted drywall, wood floor/wallpaper, and carpe ... | 2011 | 21554312 |
| lucifensin, a novel insect defensin of medicinal maggots: synthesis and structural study. | recently, we identified a new insect defensin, named lucifensin that is secreted/excreted by the blowfly lucilia sericata larvae into a wound as a disinfectant during the medicinal process known as maggot therapy. here, we report the total chemical synthesis of this peptide of 40 amino acid residues and three intramolecular disulfide bridges by using three different protocols. oxidative folding of linear peptide yielded a peptide with a pattern of disulfide bridges identical to that of native lu ... | 2011 | 21560219 |
| larval-mass effect: characterisation of heat emission by necrophageous blowflies (diptera: calliphoridae) larval aggregates. | numerous calliphoridae species larvae are necrophageous and develop on animal cadavers. during the feeding stages, a strong gregarious behaviour leads to the formation of large larval masses, allowing larvae to share digestive fluids. furthermore, a mass of larvae emits heat, resulting in a local increase of temperature. differences greater than 20°c between ambient and larval mass temperatures have already been observed, and the temperature of the mass can reach 50°c. thus, larvae could benefit ... | 2011 | 21601389 |
| decomposition and insect succession of clothed and unclothed carcasses in western australia. | the effect of clothing on carcass decomposition and patterns of insect succession onto remains were investigated in two separate years during autumn in western australia. the progression of decomposition differed between clothed and unclothed carcasses in both years of the study. the presence of clothing markedly prolonged the wet decay stage in both years with larval feeding occurring across the moist skin surface underneath clothing, as well as within and under the carcasses. ambient temperatu ... | 2011 | 21602003 |
| [a postoperative wound myiasis caused by lucilia sericata (diptera: calliphoridae) in a woman in kars.] | lucilia sericata is known as a facultative ectoparasite and it often causes myiasis in open wounds, mouth, nose and eyes of animals and rarely in humans. this case report concerns a 56 year old woman who had right breast cancer surgery. approximately 10 months after surgery, swelling, pain, redness occurred in the incision site. about two weeks later the site turned into an open wound then fly larvae (97 live larvae) were found. under t operation conditions, a large surgical wound debridement an ... | 2011 | 21618192 |
| the human aural myiasis caused by lucilia sericata. | myiasis is a rare, worldwide, human disease with seasonal variation, caused by developing larvae of a variety of fly species. it can be dangerous when infestations penetrate into the brain. in the available literature, we have found only a few papers concerning ear myiasis caused by lucilia sericata. here, we report 2 cases of aural myiasis. early intervention (surgical removal, occlusion) in these cases should prevent complications. larvae, for further examination, should be killed by immersion ... | 2011 | 21634231 |
| molecular characterization and expression pattern of an odorant receptor from the myiasis-causing blowfly, lucilia sericata (diptera: calliphoridae). | the blowfly lucilia sericata (diptera: calliphoridae) is a facultative ectoparasite that causes myiasis in both man and animals, leading to serious human health problems and economic losses in the livestock industry. like other insects, olfaction of this species plays an important role in host location and is presumably mediated by a seven transmembrane receptor family. here, we isolate and characterize lseror1, which is the first candidate member of the odorant receptor gene family from l. seri ... | 2011 | 21789580 |
| identification, analysis, and linkage mapping of expressed sequence tags from the australian sheep blowfly. | abstract: background: the australian sheep blowfly lucilia cuprina (wiedemann) (diptera: calliphoridae) is a destructive pest of the sheep, a model organism for insecticide resistance research, and a valuable tool for medical and forensic professionals. however, genomic information on l. cuprina is still sparse. results: we report here the construction of an embryonic and 2 larval cdna libraries for l. cuprina. a total of 29,816 expressed sequence tags (ests) were obtained and assembled into 7,4 ... | 2011 | 21827708 |
| livestock ectoparasites: integrated management in a changing climate. | the prevalence of livestock ectoparasites is the result of a complex interaction of factors such as parasite and host abundance, host susceptibility, climate and, critically, farmer husbandry and intervention strategies, all of which change seasonally in space and time. given the complexity of the interacting factors, the effects of any climate change on disease incidence are hard to predict, as accordingly are the optimal husbandry responses required to ameliorate any effects. here cutaneous my ... | 2011 | 21680100 |
| fatal nosocomial myiasis caused by lucilia sericata. | | 2011 | 21684632 |
| seasonal and habitat abundance and distribution of some forensically important blow flies (diptera: calliphoridae) in central california. | seasonal and habitat calliphorid abundance and distribution were examined weekly for two years (2001-2003) in santa clara county, california, using sentinel traps baited with bovine liver. of the 34,389 flies examined in three defined habitats (rural, urban, and riparian), 38% of the total catch represented compsomyiops callipes (bigot) and 23% represented phormia regina (meigen). other flies collected in this survey included calliphora vomitoria (linnaeus), calliphora latifrons (hough), lucilia ... | 2011 | 21683536 |
| the molecular systematics of blowflies and screwworm flies (diptera: calliphoridae) using 28s rrna, cox1 and ef-1α: insights into the evolution of dipteran parasitism. | the calliphoridae include some of the most economically significant myiasis-causing flies in the world - blowflies and screwworm flies - with many being notorious for their parasitism of livestock. however, despite more than 50 years of research, key taxonomic relationships within the family remain unresolved. this study utilizes nucleotide sequence data from the protein-coding genes cox1 (mitochondrial) and ef1α (nuclear), and the 28s rrna (nuclear) gene, from 57 blowfly taxa to improve resolut ... | 2011 | 21867590 |
| Population and temperature effects on Lucilia sericata (Diptera: Calliphoridae) body size and minimum development time. | Understanding how ecological conditions influence physiological responses is fundamental to forensic entomology. When determining the minimum postmortem interval with blow fly evidence in forensic investigations, using a reliable and accurate model of development is integral. Many published studies vary in results, source populations, and experimental designs. Accordingly, disentangling genetic causes of developmental variation from environmental causes is difficult. This study determined the mi ... | 2011 | 21936326 |