| ultrastructure and phylogeny of ceratomyxa diplodae (myxosporea: ceratomyxidae), from gall bladder of european seabass dicentrarchus labrax. | the myxosporean parasite ceratomyxa diplodae lubat et al. 1989 sensu sitjà-bobadilla & álvarez-pellitero, 1993, originally described from the annular seabream diplodus annularis in the adriatic sea, has subsequently been reported from several other sparid hosts, and also a moronid fish, the european seabass dicentrarchus labrax from the mediterranean sea. here, molecular identity and additional morphological data are given for this parasite infecting the gall bladder of d. labrax in a southern p ... | 2016 | 27667809 |
| gene expression profiling reveals functional specialization along the intestinal tract of a carnivorous teleostean fish (dicentrarchus labrax). | high-quality sequencing reads from the intestine of european sea bass were assembled, annotated by similarity against protein reference databases and combined with nucleotide sequences from public and private databases. after redundancy filtering, 24,906 non-redundant annotated sequences encoding 15,367 different gene descriptions were obtained. these annotated sequences were used to design a custom, high-density oligo-microarray (8 × 15 k) for the transcriptomic profiling of anterior (ai), midd ... | 2016 | 27610085 |
| assessing chronic fish health: an application to a case of an acute exposure to chemically treated crude oil. | human alteration of marine ecosystems is substantial and growing. yet, no adequate methodology exists that provides reliable predictions of how environmental degradation will affect these ecosystems at a relevant level of biological organization. the primary objective of this study was to develop a methodology to evaluate a fish's capacity to face a well-established environmental challenge, an exposure to chemically dispersed oil, and characterize the long-term consequences. therefore, we applie ... | 2016 | 27522032 |
| effects of sodium butyrate treatment on histone modifications and the expression of genes related to epigenetic regulatory mechanisms and immune response in european sea bass (dicentrarchus labrax) fed a plant-based diet. | bacteria that inhabit the epithelium of the animals' digestive tract provide the essential biochemical pathways for fermenting otherwise indigestible dietary fibers, leading to the production of short-chain fatty acids (scfas). of the major scfas, butyrate has received particular attention due to its numerous positive effects on the health of the intestinal tract and peripheral tissues. the mechanisms of action of this four-carbon chain organic acid are different; many of these are related to it ... | 2016 | 27471849 |
| effect of historical contamination in the fish community structure of a recovering temperate coastal lagoon. | this study aimed to assess the impact of trace element concentrations in fish assemblages of a recovering coastal lagoon. fish, water and sediment were sampled in winter and summer in the ria de aveiro (portugal). multivariate analyses were used to examine the relationship between fish assemblages and environmental variables (physical-chemical parameters, contaminants and sediment grain size). in winter, fish density and biomass were mainly affected by the water turbidity, while li concentration ... | 2016 | 27423444 |
| characterization of quality of sediments from paranaguá bay (brazil) by combined in vitro bioassays and chemical analyses. | the present study characterizes the quality of sediments from the paranaguá estuarine complex (south brazil). polycyclic aromatic hydrocarbons (pahs), polychlorinated biphenyls (pcbs), and organochlorine pesticides (ocps) were determined in sediment samples together with a series of different in vitro bioassays. the fish hepatoma cell line (plhc-1) was used to determine the presence of cytotoxic compounds and cyp1a- and oxidative stress-inducing agents in sediment extracts. ovarian microsomal fr ... | 2016 | 27390921 |
| intraspecific individual variation of temperature tolerance associated with oxygen demand in the european sea bass (dicentrarchus labrax). | the european sea bass (dicentrarchus labrax) is an economically important fish native to the mediterranean and northern atlantic. its complex life cycle involves many migrations through temperature gradients that affect the energetic demands of swimming. previous studies have shown large intraspecific variation in swimming performance and temperature tolerance, which could include deleterious and advantageous traits under the evolutionary pressure of climate change. however, little is known of t ... | 2016 | 27382468 |
| an oral chitosan dna vaccine against nodavirus improves transcription of cell-mediated cytotoxicity and interferon genes in the european sea bass juveniles gut and survival upon infection. | vaccines for fish need to be improved for the aquaculture sector, with dna vaccines and the oral administration route providing the most promising improvements. in this study, we have created an oral chitosan-encapsulated dna vaccine (cp-pnnv) for the nodavirus (nnv) in order to protect the very susceptible european sea bass (dicentrarchus labrax). our data show that the oral cp-pnnv vaccine failed to induce serum circulating or neutralizing specific antibodies (immunoglobulin m) or to up-regula ... | 2016 | 27370973 |
| genetic characterization and transcription analyses of the european sea bass (dicentrarchus labrax) isg15 gene. | fish interferons are cytokines involved in its resistance to viral infections by inducing the transcription of several interferon-induced genes, such as isg15. the aim of the present study was the genetic characterization of the european sea bass isg15 gene, describing the regulatory motifs found in its sequence. in addition, an in vivo analysis of transcription in response to betanodavirus (rgnnv genotype) and poly i:c has been performed. the analysis of the resulting sequences showed that sea ... | 2016 | 27368533 |
| the bromodomain testis-specific gene (brdt) characterization and expression in gilthead seabream, sparus aurata, and european seabass, dicentrarchus labrax. | multiple genes and transcription factors are involved in regulation and control of the complex process of sex determination and differentiation of fish species. also more, several hormonal factors and some environmental conditions can also be adequate spawning strategies and stimuli for inducing reproduction of fish species. brdt gene belongs to the bromodomain-extraterminal domain (bet) family of transcriptional coregulators. in mammals, brdt gene is almost exclusively expressed in testis. furt ... | 2016 | 27349318 |
| saddleback syndrome in european sea bass dicentrarchus labrax (linnaeus, 1758): anatomy, ontogeny and correlation with lateral-line, anal and pelvic fin abnormalities. | this study focused on the anatomy and ontogeny of saddleback syndrome (sbs) in reared european sea bass. the abnormality was detected at an unusually high frequency (12-94%) during a routine quality control in a commercial hatchery. anatomically the abnormality was mainly characterized by the loss of 1-5 hard spines and severe abnormalities of the proximal pterygiophores (anterior dorsal fin), size reduction of a few lepidotrichia, missing lepidotrichia and/or lepidotrichia of poor ossification ... | 2017 | 27333971 |
| life cycle inference and phylogeny of ortholinea labracis n. sp. (myxosporea: ortholineidae), a parasite of the european seabass dicentrarchus labrax (teleostei: moronidae), in a portuguese fish farm. | ortholinea labracis n. sp. is described and its life cycle is inferred from a southern portuguese fish farm, with basis on microscopic and molecular procedures. this myxosporean parasite infects the urinary bladder of the european seabass dicentrarchus labrax and the intestinal epithelium of a marine oligochaete of the genus tectidrilus. myxospores subspherical in valvular view and ellipsoidal in sutural view measuring 7.6 ± 0.3 (6.8-8.7) μm in length, 7.2 ± 0.2 (6.7-7.7) μm in width and 6.5 ± 0 ... | 2017 | 27307300 |
| concurrent environmental stressors and jellyfish stings impair caged european sea bass (dicentrarchus labrax) physiological performances. | the increasing frequency of jellyfish outbreaks in coastal areas has led to multiple ecological and socio-economic issues, including mass mortalities of farmed fish. we investigated the sensitivity of the european sea bass (dicentrarchus labrax), a widely cultured fish in the mediterranean sea, to the combined stressors of temperature, hypoxia and stings from the jellyfish pelagia noctiluca, through measurement of oxygen consumption rates (mo2), critical oxygen levels (po2crit), and histological ... | 2016 | 27301314 |
| molecular characterization and expression of na(+)/k(+)-atpase α1 isoforms in the european sea bass dicentrarchus labrax osmoregulatory tissues following salinity transfer. | the na(+)/k(+)-atpase (nka) is considered as the main pump involved in active ion transport. in the european sea bass, dicentrarchus labrax, we found two genes encoding for the alpha 1 subunit isoforms (nka α1a and nka α1b). nka α1a and nka α1b isoform amino acid (aa) sequences were compared through phylogeny and regarding key functional motifs between salmonids and other acanthomorph species. analysis of aa sequences of both isoforms revealed a high degree of conservation across teleosts. the e ... | 2016 | 27289588 |
| mapping the sex determination locus in the hāpuku (polyprion oxygeneios) using ddrad sequencing. | hāpuku (polyprion oxygeneios) is a member of the wreckfish family (polyprionidae) and is highly regarded as a food fish. although adults grow relatively slowly, juveniles exhibit low feed conversion ratios and can reach market size in 1-2 years, making p. oxygeneios a strong candidate for aquaculture. however, they can take over 5 years to reach sexual maturity in captivity and are not externally sexually dimorphic, complicating many aspects of broodstock management. understanding the sex determ ... | 2016 | 27286864 |
| repeated exposure reduces the response to impulsive noise in european seabass. | human activities have changed the acoustic environment of many terrestrial and aquatic ecosystems around the globe. mounting evidence indicates that the resulting anthropogenic noise can impact the behaviour and physiology of at least some species in a range of taxa. however, the majority of experimental studies have considered only immediate responses to single, relatively short-term noise events. repeated exposure to noise could lead to a heightened or lessened response. here, we conduct two l ... | 2016 | 27282635 |
| amino acid transporter b(0)at1 (slc6a19) and ancillary protein: impact on function. | amino acids play an important role in the metabolism of all organisms. their epithelial re-absorption is due to specific transport proteins, such as b(0)at1, a na(+)-coupled neutral amino acid symporter belonging to the solute carrier 6 family. here, a recently cloned fish orthologue, from the intestine of salmo salar, was electrophysiologically characterized with the two-electrode voltage clamp technique, in xenopus laevis oocytes heterologously expressing the transporter. substrate specificity ... | 2016 | 27255547 |
| effects of dietary carbohydrate on hepatic de novo lipogenesis in european seabass (dicentrarchus labrax l.). | farmed seabass have higher adiposity than their wild counterparts and this is often attributed to carbohydrate (cho) feeding. whether this reflects a reduction in fat oxidation, increased de novo lipogenesis (dnl), or both, is not known. to study the effects of high cho diets on hepatic tg biosynthesis, hepatic tg deuterium ((2)h) enrichment was determined following 6 days in (2)h-enriched tank water for fish fed with a no-cho control diet (ctrl), and diets with digestible starch (ds) and raw st ... | 2016 | 27247346 |
| influence of different salting processes on the evolution of the volatile metabolites of vacuum-packed fillets of farmed and wild sea bass (dicentrarchus labrax) stored under refrigeration conditions: a study by spme-gc/ms. | fish shelf-life extension is a topic of great interest. in this study the behaviour of salted and unsalted farmed and wild european sea bass (dicentrarchus labrax) fillets during storage was analysed through the evolution of their volatile metabolites. farmed and wild sea bass fillets were brine-salted for 15 or 75 min, or dry-salted, vacuum-packed and stored at 4 °c for up to 1 month, and their headspaces were studied by solid phase micro extraction-gas chromatography/mass spectrometry (spme-gc ... | 2017 | 27225021 |
| bioaccumulation and elimination of mercury in juvenile seabass (dicentrarchus labrax) in a warmer environment. | warming is an expected impact of climate change that will affect coastal areas in the future. these areas are also subjected to strong anthropogenic pressures leading to chemical contamination. yet, the consequences of both factors for marine ecosystems, biota and consumers are still unknown. the present work aims to investigate, for the first time, the effect of temperature increase on bioaccumulation and elimination of mercury [(total mercury (thg) and methylmercury (mehg)] in three tissues (m ... | 2016 | 27179934 |
| is the kisspeptin system involved in responses to food restriction in order to preserve reproduction in pubertal male sea bass (dicentrarchus labrax)? | previous works on european sea bass have determined that long-term exposure to restrictive feeding diets alters the rhythms of some reproductive/metabolic hormones, delaying maturation and increasing apoptosis during gametogenesis. however, exactly how these diets affect key genes and hormones on the brain-pituitary-gonad (bpg) axis to trigger puberty is still largely unknown. we may hypothesize that all these signals could be integrated, at least in part, by the kisspeptin system. in order to c ... | 2016 | 27164487 |
| applying quantitative and semi-quantitative histopathology to address the interaction between sediment-bound polycyclic aromatic hydrocarbons in fish gills. | even though pahs are considered priority marine pollutants, information on the interaction effects between these compounds is scarce, furthermore under ecologically-relevant circumstances. semi-quantitative and quantitative histological analyses were enforced on the gills of the seabass (dicentrarchus labrax), exposed to two model pahs, single or combined, through a series of 28-day laboratory bioassays. fish exposed to sediments contaminated with either pah (250-800ngg(-1)), isolated or combine ... | 2016 | 27117279 |
| hepcidin-dependent regulation of erythropoiesis during anemia in a teleost fish, dicentrarchus labrax. | anemia is a common disorder, characterized by abnormally low levels of red blood cells or hemoglobin. the mechanisms of anemia development and response have been thoroughly studied in mammals, but little is known in other vertebrates, particularly teleost fish. in this study, different degrees of anemia were induced in healthy european sea bass specimens (dicentrarchus labrax) and at pre-determined time points hematological parameters, liver iron content and the expression of genes involved in i ... | 2016 | 27100629 |
| importance of prebiotics in aquaculture as immunostimulants. effects on immune system of sparus aurata and dicentrarchus labrax. | infectious diseases in fish represent a major problem for the aquaculture field as they produce extensive damages and loss. over the last few years, with increased development of the aquaculture industry, different methods have been used to contrast these pathologies. common interest has led to the use of components (as additives in diets) that could contrast diseases without causing any negative impact on the environment. these components are represented by prebiotics, probiotics, and plant ext ... | 2016 | 27074444 |
| sound exposure changes european seabass behaviour in a large outdoor floating pen: effects of temporal structure and a ramp-up procedure. | underwater sound from human activities may affect fish behaviour negatively and threaten the stability of fish stocks. however, some fundamental understanding is still lacking for adequate impact assessments and potential mitigation strategies. for example, little is known about the potential contribution of the temporal features of sound, the efficacy of ramp-up procedures, and the generalisability of results from indoor studies to the outdoors. using a semi-natural set-up, we exposed european ... | 2016 | 27061472 |
| multivariate approach to gill pathology in european sea bass after experimental exposure to cadmium and terbuthylazine. | the combined use of guided quantitative expert analysis and of multivariate exploratory data analysis is reported as a robust, sensitive and sufficiently specific approach to study european sea bass gill secondary lamellar pathology after exposure to incremental doses of cadmium and terbuthylazine up to 48h. the following elementary pathological findings were considered: "epithelial lifting", "epithelial shrinkage", "epithelial swelling", "pillar cells coarctation", "pillar cells detachment", "c ... | 2016 | 27057996 |
| in vitro effects of metals on isolated head-kidney and blood leucocytes of the teleost fish sparus aurata l. and dicentrarchus labrax l. | the in vitro use of fish leucocytes to test the toxicity of aquatic pollutants, and particularly the immutoxicological effects, could be a valuable alternative to fish bioassays but has received little attention. in this study, head-kidney and peripheral blood leucocytes (hkls and pbls, respectively) from gilthead seabream (sparus aurata l.) and european sea bass (dicentrarchus labrax l.) specimens were exposed to cd, mehg (methylmercury), pb or as for 24 h being evaluated the resulting cytotoxi ... | 2016 | 27041665 |
| tetractinomyxon stages genetically consistent with sphaerospora dicentrarchi (myxozoa: sphaerosporidae) found in capitella sp. (polychaeta: capitellidae) suggest potential role of marine polychaetes in parasite's life cycle. | known life cycles of myxosporean parasites have two hosts, but very few life cycles have been disclosed, especially in the marine environment. sphaerospora dicentrarchi sitjà-bobadilla and álvarez-pellitero, 1992 is a systemic parasite from the european seabass, dicentrarchus labrax (linnaeus, 1758), a highly valuable commercial fish. it affects its health, leading to aquaculture production losses. during 2013 and 2014, an actinospore survey was conducted in a total of 5942 annelids collected fr ... | 2016 | 27041279 |
| impact of date palm fruits extracts and probiotic enriched diet on antioxidant status, innate immune response and immune-related gene expression of european seabass (dicentrarchus labrax). | the application of additives in the diet as plants or extracts of plants as natural and innocuous compounds has potential in aquaculture as an alternative to antibiotics and immunoprophylactics. the aim of the current study was to evaluate the potential effects of dietary supplementation of date palm fruit extracts alone or in combination with pdp11 probiotic on serum antioxidant status, on the humoral and cellular innate immune status, as well as, on the expression levels of some immune-related ... | 2016 | 27033470 |
| whole genome semiconductor based sequencing of farmed european sea bass (dicentrarchus labrax) mediterranean genetic stocks using a dna pooling approach. | european sea bass (dicentrarchus labrax) is an important marine species for commercial and sport fisheries and aquaculture production. recently, the european sea bass genome has been sequenced and assembled. this resource can open new opportunities to evaluate and monitor variability and identify variants that could contribute to the adaptation to farming conditions. in this work, two dna pools constructed from cultivated european sea bass were sequenced using a next generation semiconductor seq ... | 2016 | 27020381 |
| drospirenone intake alters plasmatic steroid levels and cyp17a1 expression in gonads of juvenile sea bass. | drospirenone (dro) is one of the most widely used progestins in contraceptive treatments and hormone replacement therapies. the pharmacokinetics and potential toxicological effects of dro were investigated in juvenile sea bass (dicentrarchus labrax) exposed through the diet (0.01-10 μg dro/g) for up to 31 days. dro was detected in the blood (4-27 ng/ml) of fish exposed to the highest concentration, with no significant bioaccumulation over time and no alteration of hepatic metabolizing enzymes, n ... | 2016 | 26995450 |
| local connected fractal dimension analysis in gill of fish experimentally exposed to toxicants. | an operator-neutral method was implemented to objectively assess european seabass, dicentrarchus labrax (linnaeus, 1758) gill pathology after experimental exposure to cadmium (cd) and terbuthylazine (tba) for 24 and 48h. an algorithm-derived local connected fractal dimension (lcfd) frequency measure was used in this comparative analysis. canonical variates (cva) and linear discriminant analysis (lda) were used to evaluate the discrimination power of the method among exposure classes (unexposed, ... | 2016 | 26991750 |
| gonadotropin inhibitory hormone down-regulates the brain-pituitary reproductive axis of male european sea bass (dicentrarchus labrax). | gonadotropin-inhibitory hormone (gnih) inhibits gonadotropin synthesis and release from the pituitary of birds and mammals. however, the physiological role of orthologous gnih peptides on the reproductive axis of fish is still uncertain, and their actions on the main neuroendocrine systems controlling reproduction (i.e., gnrhs, kisspeptins) have received little attention. in a recent study performed in the european sea bass, we cloned a cdna encoding a precursor polypeptide that contained c-term ... | 2016 | 26984999 |
| leptin receptor gene in the european sea bass (dicentrarchus labrax): cloning, phylogeny, tissue distribution and neuroanatomical organization. | in this study, we report the cloning of three transcripts for leptin receptor in the european sea bass, a marine teleost of economic interest. the two shortest variants, generated by different splice sites, encode all functional extracellular and intracellular domains but missed the transmembrane domain. the resulting proteins are therefore potential soluble binding proteins for leptin. the longest transcript (3605bp), termed sblepr, includes all the essential domains for binding and transductio ... | 2016 | 26979276 |
| the early stress responses in fish larvae. | during the life cycle of fish the larval stages are the most interesting and variable. teleost larvae undergo a daily increase in adaptability and many organs differentiate and become active. these processes are concerted and require an early neuro-immune-endocrine integration. in larvae communication among the nervous, endocrine and immune systems utilizes several known signal molecule families which could be different from those of the adult fish. the immune-neuroendocrine system was studied i ... | 2016 | 26968620 |
| intestinal alterations in european sea bass dicentrarchus labrax (linnaeus, 1758) exposed to microplastics: preliminary results. | this study investigates, for the first time, the intestinal responses of european sea bass dicentrarchus labrax chronically exposed to microplastics through ingestion. fish (n = 162) were fed with 3 different treatment diets for 90 days: control, native polyvinyl chloride (pvc) and polluted polyvinyl chloride (pvc) pellets. intestines were fixed and processed for histological analysis using standard techniques. histopathological alterations were examined using a score value (from 0 to 4). the di ... | 2016 | 26851981 |
| administration of a polyphenol-enriched feed to farmed sea bass (dicentrarchus labrax l.) modulates intestinal and spleen immune responses. | farmed fish are exposed to a continuous antigenic pressure by microbial and environmental agents, which may lead to a condition of chronic inflammation. in view of the notion that polyphenols, largely contained in fruits and vegetables, are endowed with antioxidant and anti-inflammatory activities, farmed sea bass (dicentrarchus labrax l.) have been administered with red grape polyphenol-enriched feed. polyphenols were extracted from the seeds of canosina nero di troia vitis vinifera and mixed w ... | 2016 | 26779301 |
| adaptation of the fish juvenile growth test (oecd tg 215, 2000) to the marine species dicentrarchus labrax. | the oecd tg 215 method (2000) (c.14 method of ec regulation 440/2008) was developed on the rainbow trout (oncorynchus mykiss) to assess chronic toxicity (28d) of chemicals on fish juveniles. it contemplates to use other well documented species identifying suitable conditions to evaluate their growth. oecd proposes the european sea bass (dicentrarchus labrax, l. 1758) as mediterranean species among vertebrates recommended in the oecd guidelines for the toxicity testing of chemicals. in this conte ... | 2016 | 26765670 |
| betanodavirus ability to infect juvenile european sea bass, dicentrarchus labrax, at different water salinity. | viral encephalopathy and retinopathy (ver) is one of the most devastating and economically relevant diseases for marine aquaculture. the presence of betanodavirus in freshwater fish is recorded, but very little is known about ver outbreaks in marine species reared in freshwater. our study investigated the ability of betanodavirus to cause disease in european sea bass, dicentrarchus labrax, reared at different salinity levels. fish were challenged with rgnnv or mock infected by bath at different ... | 2016 | 26763095 |
| germ-free sea bass dicentrarchus labrax larval model: a valuable tool in the study of host-microbe interactions. | a thorough understanding of host-microbe interactions is crucial for more efficient disease management in the marine larviculture industry. as demonstrated in terrestrial animal research, gnotobiotic systems (involving animals cultured in germ-free conditions or inoculated with known microorganisms) are excellent tools to extend our understanding of the mechanisms involved in host-microbe interactions and allow the evaluation of new treatments for diseases. in this study, we introduce a germ-fre ... | 2016 | 26758651 |
| individual variation in whole-animal hypoxia tolerance is associated with cardiac hypoxia tolerance in a marine teleost. | hypoxia is a pervasive problem in coastal environments and is predicted to have enduring impacts on aquatic ecosystems. intraspecific variation in hypoxia tolerance is well documented in fish; however, the factors underlying this variation remain unknown. here, we investigate the role of the heart in individual hypoxia tolerance of the european sea bass (dicentrarchus labrax). we found individual whole-animal hypoxia tolerance is a stable trait in sea bass for more than 18 months (duration of st ... | 2016 | 26740561 |
| tissue responsiveness to estradiol and genistein in the sea bass liver and scale. | as in mammals, estrogens in fish are essential for reproduction but also important regulators of mineral homeostasis. fish scales are a non-conventional target tissue responsive to estradiol and constitute a good model to study mineralized tissues effects and mechanisms of action of estrogenic compounds, including phytoestrogens. the responsiveness to estradiol and the phytoestrogen genistein, was compared between the scales and the liver, a classical estrogenic target, in sea bass (dicentrarchu ... | 2016 | 26718875 |
| exploring a nonmodel teleost genome through rad sequencing-linkage mapping in common pandora, pagellus erythrinus and comparative genomic analysis. | common pandora (pagellus erythrinus) is a benthopelagic marine fish belonging to the teleost family sparidae, and a newly recruited species in mediterranean aquaculture. the paucity of genetic information relating to sparids, despite their growing economic value for aquaculture, provides the impetus for exploring the genomics of this fish group. genomic tool development, such as genetic linkage maps provision, lays the groundwork for linking genotype to phenotype, allowing fine-mapping of loci r ... | 2015 | 26715088 |
| nodavirus colonizes and replicates in the testis of gilthead seabream and european sea bass modulating its immune and reproductive functions. | viruses are threatening pathogens for fish aquaculture. some of them are transmitted through gonad fluids or gametes as occurs with nervous necrosis virus (nnv). in order to be transmitted through the gonad, the virus should colonize and replicate inside some cell types of this tissue and avoid the subsequent immune response locally. however, whether nnv colonizes the gonad, the cell types that are infected, and how the immune response in the gonad is regulated has never been studied. we have de ... | 2015 | 26691348 |
| multiple vitellogenins and product yolk proteins in european sea bass (dicentrarchus labrax): molecular characterization, quantification in plasma, liver and ovary, and maturational proteolysis. | three complete vitellogenin (vtg) polypeptides of european sea bass (dicentrarchus labrax), an acanthomorph teleost spawning pelagic eggs in seawater, were deduced from cdna and identified as vtgaa, vtgab and vtgc based on current vtg nomenclature and phylogeny. label free quantitative mass spectrometry verified the presence of the three sea bass vtgs or their product yolk proteins (yps) in liver, plasma and ovary of postvitellogenic females. as evidenced by normalized spectral counts, vtgab-der ... | 2016 | 26643259 |
| noise impact on european sea bass behavior: temporal structure matters. | anthropogenic sounds come in different forms, varying not only in amplitude and frequency spectrum but also in temporal structure. although fish are sensitive to the temporal characteristics of sound, little is known about how their behavior is affected by anthropogenic sounds of different temporal patterns. we investigated this question using groups of dicentrarchus labrax (european sea bass) in an outdoor basin. our data revealed that the temporal pattern of sound exposure is important in nois ... | 2016 | 26611030 |
| pile-driving noise impairs antipredator behavior of the european sea bass dicentrarchus labrax. | in an increasingly industrialized world, man-made noise is changing the underwater acoustic environment. the effects of anthropogenic noise on marine ecosystems are not yet fully understood despite important implications for science and policy, in particular with respect to investment in offshore renewable energy. in this study, a traditional looming-stimulus experimental setup was used to investigate the acute effects of pile-driving noise on the antipredator response of european sea bass (dice ... | 2016 | 26610969 |
| effects of offshore wind farms on the early life stages of dicentrarchus labrax. | anthropogenically generated underwater noise in the marine environment is ubiquitous, comprising both intense impulse and continuous noise. the installation of offshore wind farms across the north sea has triggered a range of ecological questions regarding the impact of anthropogenically produced underwater noise on marine wildlife. our interest is on the impact on the "passive drifters," i.e., the early life stages of fish that form the basis of fish populations and are an important prey for pe ... | 2016 | 26610960 |
| acoustic stress responses in juvenile sea bass dicentrarchus labrax induced by offshore pile driving. | underwater sound generated by pile driving during construction of offshore wind farms is a major concern in many countries. this paper reports on the acoustic stress responses in young european sea bass dicentrarchus labrax (68 and 115 days old), based on four in situ experiments as close as 45 m from a pile driving activity. as a primary stress response, whole-body cortisol seemed to be too sensitive to 'handling' bias. on the other hand, measured secondary stress responses to pile driving show ... | 2016 | 26561450 |
| early genotoxic response and accumulation induced by waterborne copper, lead, and arsenic in european seabass, dicentrarchus labrax. | cu, pb, and as, which are among the most abundant metals in the aquatic environment, are also among the most health-threatened by causing diverse cellular injuries. the aim of this study was to assess and compare the potential early induction of genotoxic effects after waterborne cu, pb, and as exposure in european seabass, dicentrarchus labrax, a commercial widely cultured fish, using the micronucleus (mn) assay in peripheral blood erythrocytes. fish were exposed under laboratory conditions to ... | 2016 | 26490895 |
| european sea bass gill pathology after exposure to cadmium and terbuthylazine: expert versus fractal analysis. | the objective of this study was to compare expert versus fractal analysis as new methods to evaluate branchial lamellar pathology in european sea bass dicentrarchus labrax (linnaeus, 1758) experimentally exposed to cadmium and to terbuthylazine. in particular, guided expert quantitative and fractal analysis were performed on selected images from semithin sections to test possible differences according to exposure class (unexposed, cadmium exposed, or terbuthylazine exposed) and the discriminatio ... | 2016 | 26469527 |
| n-tio2 and cdcl2 co-exposure to titanium dioxide nanoparticles and cadmium: genomic, dna and chromosomal damage evaluation in the marine fish european sea bass (dicentrarchus labrax). | due to the large production and growing use of titanium dioxide nanoparticles (n-tio2), their release in the marine environment and their potential interaction with existing toxic contaminants represent a growing concern for biota. different end-points of genotoxicity were investigated in the european sea bass dicentrarchus labrax exposed to n-tio2 (1mgl(-1)) either alone and combined with cdcl2 (0.1mgl(-1)) for 7 days. dna primary damage (comet assay), apoptotic cells (diffusion assay), occurre ... | 2015 | 26448269 |
| gut morphology and hepatic oxidative status of european sea bass (dicentrarchus labrax) juveniles fed plant feedstuffs or fishmeal-based diets supplemented with short-chain fructo-oligosaccharides and xylo-oligosaccharides. | the effects of short-chain fructo-oligosaccharides (scfos) and xylo-oligosaccharides (xos) on gut morphology and hepatic oxidative status were studied in european sea bass juveniles weighing 60 g. fish were fed diets including fishmeal (fm diets) or plant feedstuffs (pf diets; 30 fm:70 pf) as main protein sources (control diets). four other diets were formulated similar to the control diets but including 1 % scfos or 1 % xos. at the end of the trial, fish fed pf-based diets presented histomorpho ... | 2015 | 26435350 |
| evaluation of the impact of polyethylene microbeads ingestion in european sea bass (dicentrarchus labrax) larvae. | microplastics are present in marine habitats worldwide and may be ingested by low trophic organisms such as fish larvae, with uncertain physiological consequences. the present study aims at assessing the impact of polyethylene (pe 10-45 μm) microbeads ingestion in european sea bass (dicentrarchus labrax) larvae. fish were fed an inert diet including 0, 10(4) and 10(5) fluorescent microbeads per gram from 7 until 43 days post-hatching (dph). microbeads were detected in the gastrointestinal tract ... | 2015 | 26412109 |
| kiss2 as a regulator of lh and fsh secretion via paracrine/autocrine signaling in the teleost fish european sea bass (dicentrarchus labrax). | kisspeptins are key players in the neuroendocrine control of puberty and other reproductive processes in mammals. several studies have demonstrated that the kiss/gpr54 system is expressed by gonadotrophs, but in vitro studies assessing the direct stimulatory effects of kisspeptin on gonadotropin secretion in the pituitary have provided conflicting results. in this study, we investigated whether kisspeptin directly influences the reproductive function of sea bass pituitary. first, the highly acti ... | 2015 | 26400402 |
| terminal carbohydrate composition, igm level and enzymatic and bacteriostatic activity of european sea bass (dicentrarchus labrax) skin epidermis extracts. | although the skin is one of the main defense barriers of fish to date, very little is known about the immune implications and the properties of the numerous substances present in skin cells. in the present study, terminal carbohydrate composition and some components of the skin immunity (total igm level, and several enzymatic and bacteriostatic activities) present on aqueous and organic epidermal extracts of european sea bass (dicentrarchus labrax) were determined. most of the parameters measure ... | 2015 | 26384845 |
| skin mucus proteome map of european sea bass (dicentrarchus labrax). | skin mucus is the first barrier of fish defence. proteins from skin mucus of european sea bass (dicentrarchus labrax) were identified by 2de followed by lc-ms/ms. from all the identified proteins in the proteome map, we focus on the proteins associated with several immune pathways in fish. furthermore, the real-time pcr transcript levels in skin are shown. proteins found include apolipoprotein a1, calmodulin, complement c3, fucose-binding lectin, lysozyme and several caspases. to our knowledge, ... | 2015 | 26376207 |
| dietary carbohydrate and lipid sources affect differently the oxidative status of european sea bass (dicentrarchus labrax) juveniles. | this study aimed to evaluate the effects of dietary lipid source and carbohydrate content on the oxidative status of european sea bass (dicentrarchus labrax) juveniles. for that purpose, four diets were formulated with fish oil (fo) and vegetable oils (vo) as the lipid source and with 20 or 0 % gelatinised starch as the carbohydrate source, in a 2×2 factorial design. liver and intestine antioxidant enzyme activities (catalase (cat), superoxide dismutase (sod), glutathione peroxidase (gpx), gluta ... | 2015 | 26365262 |
| effects of fish oil replacement by vegetable oil blend on digestive enzymes and tissue histomorphology of european sea bass (dicentrarchus labrax) juveniles. | the impact of replacing circa 70% fish oil (fo) by a vegetable oil (vo) blend (rapeseed, linseed, palm oils; 20:50:30) in diets for european sea bass juveniles (ibw 96 ± 0.8 g) was evaluated in terms of activities of digestive enzymes (amylase, lipase, alkaline phosphatase, trypsin and total alkaline proteases) in the anterior (ai) and posterior (pi) intestine and tissue morphology (pyloric caeca-pc, ai, pi, distal intestine-di and liver). for that purpose, fish were fed the experimental diets f ... | 2016 | 26364216 |
| in vitro immunotoxicological effects of heavy metals on european sea bass (dicentrarchus labrax l.) head-kidney leucocytes. | the knowledge about the direct effects of heavy metals on fish leucocytes is still limited. we investigate the in vitro effects of heavy metals (cd, hg, pb or as) on oxidative stress, viability and innate immune parameters of head-kidney leucocytes (hkls) from european sea bass (dicentrarchus labrax). production of free oxygen radicals was induced by cd, hg and as, mainly after 30 min of exposure. cd and hg promoted both apoptosis and necrosis cell death while pb and as did only apoptosis, in al ... | 2015 | 26363228 |
| a new snp-based vision of the genetics of sex determination in european sea bass (dicentrarchus labrax). | european sea bass (dicentrarchus labrax) is one of the most important farmed species in mediterranean aquaculture. the observed sexual growth and maturity dimorphism in favour of females adds value towards deciphering the sex determination system of this species. current knowledge indicates the existence of a polygenic sex determining determination system that interacts with temperature. this was explored by restriction-site associated dna (rad) marker analysis in a test panel of 175 offspring t ... | 2015 | 26337592 |
| new insights into the factors mediating the onset of puberty in sea bass. | in populations of 1-year-old male european sea bass (dicentrarchus labrax), only large males are able to acquire for the first time a functional competence of their reproductive axis; in other words, to attain puberty. to examine the causes and mechanisms involved in the onset of puberty in this species, a size sorting sampling was carried out to obtain two experimental groups of small and large male fish exhibiting different growth rates. as expected, only large fish reached full spermiogenesis ... | 2015 | 26315387 |
| dietary carbohydrate and lipid source affect cholesterol metabolism of european sea bass (dicentrarchus labrax) juveniles. | plant feedstuffs (pf) are rich in carbohydrates, which may interact with lipid metabolism. thus, when considering dietary replacement of fishery by-products with pf, knowledge is needed on how dietary lipid source (ls) and carbohydrates affect lipid metabolism and other metabolic pathways. for that purpose, a 73-d growth trial was performed with european sea bass juveniles (ibw 74 g) fed four diets differing in ls (fish oil (fo) or a blend of vegetable oils (vo)) and carbohydrate content (0 % (c ... | 2015 | 26306559 |
| dietary mannan oligosaccharides: counteracting the side effects of soybean meal oil inclusion on european sea bass (dicentrarchus labrax) gut health and skin mucosa mucus production? | the main objective of this study was to assess the effects of 4 g kg(-1) dietary mannan oligosaccharides (mos) inclusion in soybean oil (sbo)- and fish oil (fo)-based diets on the gut health and skin mucosa mucus production of european sea bass juveniles after 8 weeks of feeding. dietary mos, regardless of the oil source, promoted growth. the intestinal somatic index was not affected, however dietary sbo reduced the intestinal fold length, while dietary mos increased it. the dietary oil source f ... | 2015 | 26300883 |
| sensitive periods for 17β-estradiol exposure during immune system development in sea bass head kidney. | an increasing body of evidence suggests that sex steroids play an important role in the development and regulation of vertebrate immune defense. therefore, compounds with estrogenic activity may influence the immune system via receptor-mediated pathways. the presence of estrogen receptors in immune cells and organs during the early stages of development may indicate that female steroid hormones are involved in the maturation of the fish immune system. this is of particular importance, as some ma ... | 2016 | 26278277 |
| multiple hepcidins in a teleost fish, dicentrarchus labrax: different hepcidins for different roles. | teleost fish rely heavily on their innate immunity for an adequate response against pathogens and environmental challenges, with the production of antimicrobial peptides being one of their first lines of defense. among those is hepcidin, a small cysteine-rich antimicrobial peptide that is also the key regulator of iron metabolism. although most mammals possess a single hepcidin gene, with a dual role in both iron metabolism regulation and antimicrobial response, many teleost fish present multipl ... | 2015 | 26268656 |
| subfunctionalization of arylalkylamine n-acetyltransferases in the sea bass dicentrarchus labrax: two-ones for one two. | melatonin is an important component of the vertebrates circadian system, synthetized from serotonin by the successive action of the arylalkylamine n-acetyltransferase (aanat: serotonin→n-acetylserotonin) and acetylserotonin-o-methyltransferase (asmt: n-acetylserotonin→melatonin). aanat is responsible for the daily rhythm in melatonin production. teleost fish are unique because they express two aanat genes, aanat1 and aanat2, mainly expressed in the retina and pineal gland, respectively. in silic ... | 2015 | 26267754 |
| effect of xylan oligosaccharides generated from corncobs on food acceptability, growth performance, haematology and immunological parameters of dicentrarchus labrax fingerlings. | the objective of this study was to determine the effect of two levels of inclusion of xylan oligosaccharides (xos) extracted from corncob on growth, feed utilization, immune status and disease resistance of mediterranean sea bass (dicentrarchus labrax) fingerlings. specimens of 4.75 ± 0.69 g at initial density of 2.7 ± 0.13 kg/m(3) were fed during 12 weeks at 0 g kg(-1) diet, 5 g kg(-1) diet and 10 g kg(-1) diet, dietary xos level of inclusion in a commercial sea bass diet. feeding the fish at b ... | 2015 | 26242460 |
| nutritional status as the key modulator of antioxidant responses induced by high environmental ammonia and salinity stress in european sea bass (dicentrarchus labrax). | salinity fluctuation is one of the main factors affecting the overall fitness of marine fish. in addition, water borne ammonia may occur simultaneously with salinity stress. additionally, under such stressful circumstances, fish may encounter food deprivation. the physiological and ion-osmo regulatory adaptive capacities to cope with all these stressors alone or in combination are extensively addressed in fish. to date, studies revealing the modulation of antioxidant potential as compensatory re ... | 2015 | 26241315 |
| combination effects of nano-tio2 and 2,3,7,8-tetrachlorodibenzo-p-dioxin (tcdd) on biotransformation gene expression in the liver of european sea bass dicentrarchus labrax. | the aim of present study was to investigate the influence of titanium dioxide nanoparticles (nano-tio2, aeroxide® p25) on 2,3,7,8-tetrachlorodibenzo-p-dioxin (tcdd) dependent biotransformation gene expression in liver of juvenile european sea bass dicentrarchus labrax. an in vivo 7day waterborne exposure was performed with nano-tio2 (1mg/l) and 2,3,7,8-tcdd (46pg/l), singly and in combination. the mrna expression of aryl hydrocarbon receptor repressor (ahrr), estrogen receptor (erβ2), abc transp ... | 2016 | 26235595 |
| modulation of the expression of components of the stress response by dietary arachidonic acid in european sea bass (dicentrarchus labrax) larvae. | this study reports for the first time on european sea bass, dicentrarchus labrax (l.), larvae, the effect of different levels of dietary arachidonic acid (ara; 20:4n-6) on the expression of genes related to the fish stress response. copies of mrna from genes related to steroidogenesis [star (steroidogenic acute regulatory protein), c-fos, and cyp11β (11β-hydroxylase gene)], glucocorticoid receptor complex [gr (glucocorticoid receptor) and hsp (heat shock proteins) 70 and 90) and antioxidative st ... | 2015 | 26233819 |
| calcium response of kcl-excited populations of ventricular myocytes from the european sea bass (dicentrarchus labrax): a promising approach to integrate cell-to-cell heterogeneity in studying the cellular basis of fish cardiac performance. | climate change challenges the capacity of fishes to thrive in their habitat. however, through phenotypic diversity, they demonstrate remarkable resilience to deteriorating conditions. in fish populations, inter-individual variation in a number of fitness-determining physiological traits, including cardiac performance, is classically observed. information about the cellular bases of inter-individual variability in cardiac performance is scarce including the possible contribution of excitation-con ... | 2015 | 26205950 |
| stanniocalcin 1 effects on the renal gluconeogenesis pathway in rat and fish. | the mammalian kidney contributes significantly to glucose homeostasis through gluconeogenesis. considering that stanniocalcin 1 (stc1) regulates atp production, is synthesized and acts in different cell types of the nephron, the present study hypothesized that stc1 may be implicated in the regulation of gluconeogenesis in the vertebrate kidney. human stc1 strongly reduced gluconeogenesis from (14)c-glutamine in rat renal medulla (md) slices but not in renal cortex (cx), nor from (14)c-lactic aci ... | 2015 | 26187698 |
| lpxrfa peptide system in the european sea bass: a molecular and immunohistochemical approach. | gonadotropin-inhibitory hormone (gnih) is a neuropeptide that suppresses reproduction in birds and mammals by inhibiting gnrh and gonadotropin secretion. gnih orthologs with a c-terminal lpxrfamide (lpxrfa) motif have been identified in teleost fish. although recent work also suggests its role in fish reproduction, studies are scarce and controversial, and have mainly focused on cyprinids. in this work we cloned a full-length cdna encoding an lpxrfa precursor in the european sea bass, dicentrarc ... | 2016 | 26105807 |
| innate immunity and antioxidant systems in different tissues of sea bass (dicentrarchus labrax) exposed to crude oil dispersed mechanically or chemically with corexit 9500. | the aim of the study was to evaluate effects of chemically dispersed oil by the dispersant corexit 9500 on innate immunity and redox defenses in a marine model fish. sea bass (dicentrarchus labrax) were exposed 48h to four experimental conditions: a control group (c), a group only exposed to the dispersant (d; 3.6mg/l) and two groups exposed to 80mg/l oil mechanically or chemically dispersed (md; cd). alternative pathway of complement activity and lysozyme concentration was measured in plasma in ... | 2015 | 26093109 |
| growth and immune system performance to assess the effect of dispersed oil on juvenile sea bass (dicentrarchus labrax). | the potential impact of chemically and mechanically dispersed oil was assessed in a model fish of european coastal waters, the sea bass dicentrarchus labrax. juvenile sea bass were exposed for 48h to dispersed oil (mechanically and chemically) or dispersants alone. the impact of these exposure conditions was assessed using growth and immunity. the increase observed in polycyclic aromatic hydrocarbon metabolites in bile indicated oil contamination in the fish exposed to chemical and mechanical di ... | 2015 | 26092553 |
| modulation of adrenocorticotrophin hormone (acth)-induced expression of stress-related genes by pufa in inter-renal cells from european sea bass (dicentrarchus labrax). | dietary fatty acids have been shown to exert a clear effect on the stress response, modulating the release of cortisol. the role of fatty acids on the expression of steroidogenic genes has been described in mammals, but little is known in fish. the effect of different fatty acids on the release of cortisol and expression of stress-related genes of european sea bass (dicentrarchus labrax) head kidney, induced by a pulse of adenocorticotrophin hormone (acth), was studied. tissue was maintained in ... | 2015 | 26090096 |
| impulsive sounds change european seabass swimming patterns: influence of pulse repetition interval. | seismic shootings and offshore pile-driving are regularly performed, emitting significant amounts of noise that may negatively affect fish behaviour. the pulse repetition interval (pri) of these impulsive sounds may vary considerably and influence the behavioural impact and recovery. here, we tested the effect of four pris (0.5-4.0s) on european seabass swimming patterns in an outdoor basin. at the onset of the sound exposures, the fish swam faster and dived deeper in tighter shoals. pri affecte ... | 2015 | 26088542 |
| high environmental ammonia elicits differential oxidative stress and antioxidant responses in five different organs of a model estuarine teleost (dicentrarchus labrax). | we investigated oxidative status and antioxidant profile in five tissues (brain, liver, gills, muscle and kidney) of european sea bass (dicentrarchus labrax) when exposed to high environmental ammonia (hea, 20 mg/l~1.18 mm as nh4hco3) for 12 h, 2 days, 3.5 days, 7.5 days and 10 days. results show that hea triggered ammonia accumulation and induced oxidative stress in all tissues. unlike other organs, hydrogen peroxide (h2o2) and malondialdehyde (mda) accumulation in liver were restored to contro ... | 2015 | 26073360 |
| hepatic biotransformation and antioxidant enzyme activities in mediterranean fish from different habitat depths. | marine fish are threatened by anthropogenic chemical discharges. however, knowledge on adverse effects on deep-sea fish or their detoxification capabilities is limited. herein, we compared the basal activities of selected hepatic detoxification enzymes in several species (solea solea, dicentrarchus labrax, trachyrhynchus scabrus, mora moro, cataetix laticeps and alepocehalus rostratus) collected from the coast, middle and lower slopes of the blanes canyon region (catalan continental margin, nw m ... | 2015 | 26070027 |
| high diversity of skin-associated bacterial communities of marine fishes is promoted by their high variability among body parts, individuals and species. | animal-associated microbiotas form complex communities, which are suspected to play crucial functions for their host fitness. however, the biodiversity of these communities, including their differences between host species and individuals, has been scarcely studied, especially in case of skin-associated communities. in addition, the intraindividual variability (i.e. between body parts) has never been assessed to date. the objective of this study was to characterize skin bacterial communities of ... | 2015 | 26048284 |
| effects of multiple freezing (-18 ± 2 °c) and microwave thawing cycles on the quality changes of sea bass (dicentrarchus labrax). | sensory, chemical [ph, total volatile basic nitrogen (tvb-n), trimethylamine (tma-n), thiobarbituric acid (tba)] and composition (crude protein and crude lipid) analyses were carried out on whole, gutted and fillets of seabass that were frozen (-18 ± 2 °c) and thawed more than once in microwave. it was detected that the sensorial acceptability values decreased certainly for all fish samples (whole, gutted and fillets of sea bass) when compared with control group. while chemical (ph, tvb-n, tma-n ... | 2015 | 26028727 |
| gonadotropins in european sea bass: endocrine roles and biotechnological applications. | follicle stimulating hormone (fsh) and luteinizing hormone (lh) are central endocrine regulators of the gonadal function in vertebrates. they act through specific receptors located in certain cell types found in the gonads. in fish, the differential roles of these hormones are being progressively elucidated due to the development of suitable tools for their study. in european sea bass (dicentrarchus labrax), isolation of the genes coding for the gonadotropin subunits and receptors allowed in fir ... | 2015 | 26002037 |
| microbiological spoilage and volatiles production of gutted european sea bass stored under air and commercial modified atmosphere package at 2 °c. | microbiological, sensory, tvb-n and tma-n changes and volatile organic compounds (vocs) detection using the spme/gc-ms technique, were performed to evaluate potential chemical spoilage indices (csi) of gutted sea bass (dicentrarchus labrax) stored at 2 °c under air and in modified atmosphere packaging (map co2: 60%, o2: 10%, n2: 30%). shelf-life, determined by sensory evaluation, of gutted sea bass stored at 2 °c under air and map was 9 and 13 d respectively. pseudomonas and h2s producing bacter ... | 2015 | 25998814 |
| contribution of dietary starch to hepatic and systemic carbohydrate fluxes in european seabass (dicentrarchus labrax l.). | in the present study, the effects of partial substitution of dietary protein by digestible starch on endogenous glucose production were evaluated in european seabass (dicentrarchus labrax). the fractional contribution of dietary carbohydrates v. gluconeogenesis to blood glucose appearance and hepatic glycogen synthesis was quantified in two groups of seabass fed with a diet containing 30% digestible starch (ds) or without a carbohydrate supplement as the control (ctrl). measurements were perform ... | 2015 | 25989995 |
| metals content in otoliths of dicentrarchus labrax from two fish farms of sicily. | otoliths of cultured sea bass (dicentrarchus labrax) from two different fish farms of sicily were collected and analyzed by using inductively coupled plasma optical emission spectroscopy. metal content (ba, cd, fe, mg, mn, sr, and zn) was measured in order to test the potential use of biogenic carbonates as proxies of dissimilar environmental conditions since the fish farms are implanted in opposite coastal marine areas (gulf of castellammare and gulf of gela) characterized by different oceanogr ... | 2015 | 25980727 |
| effect of dispersed crude oil on cardiac function in seabass dicentrarchus labrax. | in this study, the impact of dispersed oil was assessed in dicentrarchus labrax, a fish frequently used as an oil contamination indicator species. fish were exposed for 48h to (mechanically and chemically) dispersed oil and dispersant alone. the impact of these exposure conditions was assessed on cardiac function by measuring (i) the contraction strength, the contraction and the relaxation speeds (ii) the cardiac energy metabolism using respirometry on permeabilized cardiac fibers. compared to c ... | 2015 | 25950135 |
| a new apeh cluster with antioxidant functions in the antarctic hemoglobinless icefish chionodraco hamatus. | acylpeptide hydrolase (apeh) is a ubiquitous cytosolic protease that plays an important role in the detoxification of oxidised proteins. in this work, to further explore the physiological role of this enzyme, two apeh cdnas were isolated from the chionodraco hamatus icefish, which lives in the highly oxygenated antarctic marine environment. the encoded proteins (apeh-1(ch) and apeh-2(ch)) were characterised in comparison with the uniquely expressed isoform from the temperate fish dicentrarchus l ... | 2015 | 25946123 |
| oxidative stress, genotoxicity and histopathology biomarker responses in mugil cephalus and dicentrarchus labrax gill exposed to persistent pollutants. a field study in the bizerte lagoon: tunisia. | the use of biomarkers has become an important tool for modern environmental assessment as they can help to predict pollutants involved in the monitoring program. despite the importance of fish gill in several functions (gaseous exchange, osmotic and ionic regulation, acid-base balance and nitrogenous waste) its use in coastal water biomonitoring focusing on protection and damage is scarce. this field study investigates biochemical (catalase, superoxide dismutase, lipid peroxidation), molecular ( ... | 2015 | 25912422 |
| characterization of the annual regulation of reproductive and immune parameters on the testis of european sea bass. | the european sea bass, dicentrarchus labrax l., is a seasonal gonochoristic species, the males of which are generally mature during their second year of life. it has been demonstrated that cytokines and immune cells play a key role in the testicular development. this reproductive-immune interaction might be very important in the sea bass since several pathogens are able to colonise the gonad and persist in this tissue, altering further reproductive functions and spreading disease. this study aim ... | 2015 | 25896883 |
| evaluation of different methods of stunning/killing sea bass (dicentrarchus labrax) by tissue stress/quality indicators. | the aim of the study was to evaluate the effect on the final product quality of certain innovative stunning/killing methods for sea bass as substitutes for the most common methods used by european farmers. the changes in tissue stress/quality parameters were monitored from the first hours after death and during the shelf life of the fish. two trials were conducted in july and november on n. 231 sea bass stunned/killed by ice-water slurry, by single gas or mixture of gases in ice-water and by sin ... | 2015 | 25892757 |
| rapid extraction and reverse phase-liquid chromatographic separation of mercury(ii) and methylmercury in fish samples with inductively coupled plasma mass spectrometric detection applying oxygen addition into plasma. | a simple and sensitive procedure was developed for extraction and speciation of mercury in fish. species separation was accomplished with reverse phase-high performance liquid chromatography (hplc) hyphenated to inductively coupled plasma mass spectrometry (icp-ms). oxygen addition into plasma allowed use of organic-rich mobile phase, achieving species separation in 4 min. mercury species extraction was achieved by microwave exposure for 2 min at mild conditions (60°c, ph 2.0), avoiding necessit ... | 2015 | 25872437 |
| farmed and wild sea bass (dicentrarchus labrax) volatile metabolites: a comparative study by spme-gc/ms. | farmed and wild european sea bass (dicentrarchus labrax) could be distinguished by its volatile metabolites, an issue not addressed until now. the aim of this work was to study these metabolites by solid-phase microextraction followed by gas chromatography/mass spectrometry (spme-gc/ms). | 2016 | 25851130 |
| do experimental units of different scale affect the biological performance of european sea bass dicentrarchus labrax larvae? | the effects of different tank volumes (2000, 500 and 40 l) on european sea bass dicentrarchus labrax larval rearing, relating to growth, survival, quality and stress variables, were investigated. a dynamic energy budget (deb) model was used to analyse the results. the hydrodynamics of the tanks exhibited differences, with the water currents in the 2000 l tanks to be almost one order of magnitude stronger than those in the 40 l ones. important differences in fish growth were observed between smal ... | 2015 | 25846855 |
| nanoparticle-based lateral flow biosensor for visual detection of fish nervous necrosis virus amplification products. | lateral flow paper biosensors are an attractive analytical platform for detection of human and veterinary disease pathogens because they are optimal for accurate, rapid and sensitive analysis in research laboratory setups, as well as field analysis. since diseases of viral etiology have been wreaking havoc in aquaculture industry, as well as the environment, the present study aims at the development of a gold nanoparticle-based biosensor for fish nervous necrosis virus (nodavirus) nucleic acids ... | 2015 | 25797786 |
| lack of evidence for a role of olfaction on first maturation in farmed sea bass dicentrarchus labrax. | chemical communication is widespread in the animal kingdom and olfaction constitutes a powerful channel for social and environmental cues. in fish, olfactory stimuli are known to influence physiological processes, including reproduction. here we investigate the effects of olfaction on puberty in european sea bass dicentrarchus labrax males. intact sea bass coming to first maturity (puberty) are able to smell conspecific odours. however, induced anosmia during most of the spermatogenesis period h ... | 2015 | 25736451 |
| dynamics of gene expression patterns during early development of the european seabass (dicentrarchus labrax). | larval and embryonic stages are the most critical period in the life cycle of marine fish. key developmental events occur early in development and are influenced by external parameters like stress, temperature, salinity, and photoperiodism. any failure may cause malformations, developmental delays, poor growth, and massive mortalities. advanced understanding of molecular processes underlying marine larval development may lead to superior larval rearing conditions. today, the new sequencing and b ... | 2015 | 25736025 |
| lectins with potential for anti-cancer therapy. | this article reviews lectins of animal and plant origin that induce apoptosis and autophagy of cancer cells and hence possess the potential of being developed into anticancer drugs. apoptosis-inducing lectins encompass galectins, c-type lectins, annexins, haliotis discus discus lectin, polygonatum odoratum lectin, mistletoe lectin, and concanavalin a, fucose-binding dicentrarchus labrax lectin, and strongylocentrotus purpuratus lectin, polygonatum odoratum lectin, and mistletoe lectin, polygonat ... | 2015 | 25730388 |
| effects of carcinogenic versus non-carcinogenic ahr-active pahs and their mixtures: lessons from ecological relevance. | polycyclic aromatic hydrocarbons (pahs) are priority environmental mutagens and carcinogens that occur in the aquatic environment as mixtures rather than the individual compounds for which guidelines are issued. the present work aimed at understanding the interaction effects between carcinogenic and non-carcinogenic pahs in a model marine fish (dicentrarchus labrax) in realistic scenarios. laboratory assays under ecologically-relevant parameters were conducted for 28 days with sediments spiked w ... | 2015 | 25704830 |
| exogenous 17β-oestradiol (e2) modifies thymus growth and regionalization in european sea bass dicentrarchus labrax. | the effect of 17β-oestradiol (e2) on the growth of the thymus and its regionalization into cortex and medulla was investigated in juvenile european sea bass dicentrarchus labrax as they find themselves close to sources of oestrogenic pollution whilst residing in their estuarine nursery areas. while the exposure to 2, 20 and 200 ng l(-1) in 60 days post-hatch (dph) fish tended to cause a non-monotonous dose-response curve with a significant difference of the cortex size between lowest and highest ... | 2015 | 25683570 |