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[the infectious bronchitis virus in chickens and methods of diagnosis of the disease]. 19654959998
genetic differences among chicken embryos in response to inoculation with an isolate of infectious bronchitis virus. 19664960463
avian infectious bronchitis--virus distribution in tissues of chicks. 19664960464
a comparative study of single and multiple respiratory infections in the chicken: multiple infections (with mycoplasma gallisepticum, newcastle disease virus, and infectious bronchitis virus). 19664961637
response of chickens inoculated with infectious bronchitis virus to temperature and humidity environments. 19674961988
thermal stability of infectious bronchitis virus in the presence of salt solutions. 19674962422
synergism between avian infectious bronchitis virus and haemophilus gallinarum. 19674962423
infectious bronchitis virus interference with newcastle disease virus in monolayers of chicken kidney cells. 19674964019
immunofluorescence of avian infectious bronchitis virus in primary chicken embryo kidney, liver, lung, and fibroblast cell cultures. 19664966187
infectious bronchitis virus interference with growth of newcastle disease virus. i. study of interference in chicken embryos. 19634966225
serologic differences between the beaudette and massachusetts strains of infectious bronchitis virus. 19634966446
neurtralising antibodies for avian infectious bronchitis virus in queensland poultry flocks. 19684966718
titration of avian infectious bronchitis virus in the tissues of experimentally infected chickens. 19674966928
antigenic characterization of infectious bronchitis virus. 19684967045
morphogenosis of avian infectious bronchitis virus in chicken embryo fibroblasts. 19684974938
isolation from man of "avian infectious bronchitis virus-like" viruses (coronaviruses) similar to 229e virus, with some epidemiological observations. 19694976345
purification and further characterization of an "ibv-like" virus (coronavirus). 19704991400
a soluble antigen of infectious bronchitis virus. 19704992074
antibody-neutralized avian infectious bronchitis virus in chicken embryo kidney cells: entry and degradation. 19704992536
[preliminary study on the diagnosis of avian infectious bronchitis by fluorescent antibody technic]. 19714997553
antigenic variation in strains of avian infectious bronchitis virus. 19714999979
the use of the gel diffusion precipitin technique with avian infectious bronchitis nephritis virus. 19715001539
the immune response to infectious bronchitis virus determined by respiratory signs, virus infection, and histopathological lesions. 19725016523
chemical characterization of avian infectious bronchitis virus receptor sites. 19725023057
the effect of parameter selection in evaluation of infectious bronchitis virus vaccine. i. evaluation of the connecticut strain by virus recovery tests. 19725038646
the effect of parameter selection in evaluation of infectious bronchitis virus vaccine. ii. effect of single vs. serial passage of tracheal swab material, and of the positive-negative vs. a point-value system in reporting results. 19725038655
attachment of avian infectious bronchitis virus to chicken embryo kidney cells. 19725066126
some characteristics of isolates of infectious bronchitis virus from commercial vaccines. 19725079872
immune response in chickens to infectious bronchitis virus, strain 33. 3. beta-propiolactone-inactivated and live-virus vaccines compared by statistical methods. 19725079874
recovery of infectious bronchitis virus from eggs and chicks produced by experimentally inoculated hens. 19715091661
the site of replication of infectious bronchitis virus in the oviduct of experimentally infected hens. 19715098189
characteristics of an isolate of infectious bronchitis virus from chickens in florida. 19715104155
characterization of a new infectious bronchitis virus isolate. ii. some chemical and physical properties of clark 333. 19715107294
isolation of infectious bronchitis virus from newborn chicks and dead-in-shell embryos. 19715168509
isolation and characteristics of avian nephrosis-inducing infectious bronchitis virus (coronavirus). 19715169458
direct complement fixation test with avian infectious bronchitis virus in chicken.the direct complement fixation test was performed to follow the antibody response in chickens infected with avian infectious bronchitis virus. concentrated allantoic fluid (4 units) was used as an antigen and allowed to react with serially diluted antiserum in the presence of two complete units of guinea-pig complement for 3 hr. at 4 degrees c. and (1/2) hr. at 37 degrees c. before the addition of sensitized cells. serum was unheated and used either fresh or within one month of storage at -30 de ...19705270203
avian infectious bronchitis: the protection afforded by an inactivated virus vaccine. 19695388980
effects of avian infectious bronchitis virus (ibv) on tracheal organ cultures. 19695391213
mixed infection with infectious bronchitis virus and mycoplasma gallisepticum in a chicken flock. 19695392003
[observations and research on the immunizing effectiveness of an inactivated vaccine against avian infectious bronchitis]. 19695406594
chemical pathology of nephrosis induced by an infectious bronchitis virus. 19705434441
viral susceptibility range of the fathead minnow (pimephales promelas) poikilothermic cell line.the viral susceptibility range of a poikilothermic cell line derived from the fathead minnow (pimephales promelas) (fhm) to infection by a number of homoiothermic viruses representing most of the presently recognized viral groups and a member of the psittacosis-lymphogranuloma-trachoma group of agents was studied. all infectious agents, except poliovirus types 1 and 3, infectious bursal agent, and an avian infectious bronchitis virus (ibv) strain, readily multiplied in the fhm cell culture syste ...19705461163
studies on australian infectious bronchitis virus. iv. apparent farm-to-farm airborne transmission of infectious bronchitis virus. 19705461570
large-quantity production of chicken embryo tracheal organ cultures and use in virus and mycoplasma studies.chicken tracheal organ cultures were made from embryos which were 19 to 20 days old. transversely cut rings of trachea were placed in screw-capped tissue-culture tubes with eagle's-n-2-hydroxyethylpiperazine-n'-2-ethanesulfonic acid (hepes) medium and incubated in roller drums. the method had advantages over other organ culture systems in that these cultures were prepared in numbers similar to conventional tissue cultures, ciliary activity was quickly and accurately evaluated, and contamination ...19705463183
reference collection of strains of avian infectious bronchitis virus. 19705463723
[absence of cross immunity due respectively to connecticut and massachusetts strains of fowl infectious bronchitis virus]. 19705466589
response of chicken vaccinated with live infectious bronchitis virus vaccine to killed newcastle disease vaccine. 19705471578
an electron-microscope study of the trachea of the fowl infected with avian infectious bronchitis virus. 19705505021
[action of benzydamine on chickens infected by intranasal administration of avian infectious bronchitis virus. (preliminary note)]. 19705517452
avian infectious bronchitis in hong kong. 19705530632
pathogenesis of oviduct lesions in immature chickens following exposure to infectious bronchitis virus at one day old. 19715547754
abnormalities in laying chickens following exposure to infectious bronchitis virus at one day old. 19715547755
interaction of mycoplasma gallisepticum, mild strains of newcastle disease virus and infectious bronchitis virus in chickens. 19715569701
[infectious bronchitis of chickens. iv. characterization of new field strain of ib virus (ibv-10)]. 19675594731
[serological study of the spread in italy of different types of the virus of avian infectious bronchitis]. 19675596592
morphogenesis of avian infectious bronchitis virus and a related human virus (strain 229e).avian infectious bronchitis virus (ibv) and strain 229e, a virus recently recovered from patients with colds, have been shown to possess a similar distinctive morphology in negatively stained preparations. an electron microscopic study of the morphogenesis of ibv in the chorioallantoic membrane and of strain 229e in wi-38 cells was performed. in infected cells, round electron-dense particles 82 mmu in diameter were observed to form by a process of budding from membranes of the endoplasmic reticu ...19675630226
the morphological and biological effects of various antisera on avian infectious bronchitis virus. 19685692879
an interference type of serological test for infectious bronchitis virus using newcastle disease virus. 19685699787
replication and cytopathogenicity of avian infectious bronchitis virus in chicken embryo kidney cells. 19685729633
neutralization of infectious bronchitis virus by human sera. 19685748864
the pathogenicity of an infectious bronchitis virus for laying hens, with observations on pathogenesis. 19685750632
neutralizing antibody complex of infectious bronchitis virus. 19695773320
studies on avian infectious bronchitis virus. i. distribution and survival of the virus in tissues of affected chickens and studies on the carrier status. 19695806095
studies on avian infectious bronchitis virus. 2. incidence of the virus in broiler and layer flocks, by isolation and serological methods. 19695806096
the effect of unilateral ureteral ligation on mortality in chickens experimentally infected with nephrosis-inducing infectious bronchitis virus. 19695815412
[comparative studies on the pathogenicity, histo-pathogenesis and immunizing ability of two attenuated strains of avian infectious bronchitis virus]. 19695815857
serologic and immunologic properties of a recent isolate of infectious bronchitis virus. 19695816048
[differentiation of field strains of avian bronchitis virus (ibv)]. 19695817689
titration of infectious bronchitis virus in tracheas of susceptible and immune chickens. 19695818062
[research on the sensitivity of various small birds to avian infectious bronchitis virus]. 19666012003
adaptation of the massachusetts strain of infectious bronchitis virus to turkey embryos. 19676067604
immunity following aerosol exposure to high-embryo-passage avian infectious bronchitis virus. 19676069470
electron microscopy of infectious bronchitis virus. 19676070456
a leader sequence is present on mrna a of avian infectious bronchitis virus.the subgenomic mrnas of the fowl coronavirus infectious bronchitis virus (ibv) form a 3' co-terminal or 'nested' set. the rnase t1 oligonucleotide mapping data which demonstrated this form of sequence organization provided no evidence for the presence of non-contiguous (leader) sequences fused to the 5' termini of the mrnas. however, we have been able to demonstrate, by extension of an end-labelled synthetic oligonucleotide primer, the presence of a leader sequence on ibv mrna a. sequencing by t ...19846086827
sequential development of humoral immunity and assessment of protection in chickens following vaccination and challenge with avian infectious bronchitis virus.chicks without specific maternal antibody to avian infectious bronchitis virus were vaccinated intranasally at two weeks old with a live h120 vaccine and a proportion revaccinated with a live h52 vaccine at 15 weeks. groups of these birds were then challenged intratracheally with the massachusetts m41 strain at varying intervals and their humoral antibody responses monitored by neutralisation and haemagglutination-inhibition tests. protection was assessed by the presence of tracheal ciliostasis ...19846089286
occurrence and significance of infectious bronchitis virus variant strains in egg and broiler production in the netherlands.despite vaccination against infectious bronchitis virus (ibv) with the massachusetts type vaccine viruses h120 and h52 in the netherlands, an increasing number of properly vaccinated flocks have suffered from the disease since 1978. in the years 1978-1982, the virus was isolated from 162 ibv suspected flocks. cross-virus-neutralization tests showed that the majority (67 per cent) of these isolates belonged to serotypes other than the massachusetts type, the connecticut-, florida-, iowa 97-, iowa ...19846091319
in vitro and in vivo studies with an avian reovirus derived from a temperature-sensitive mutant clone.a temperature-sensitive (ts) mutant of avian reovirus isolate maine 1-203 was obtained at 41 c. the 19th passage of this mutant (ts 19) was utilized for in vitro studies comparing its replication in macrophage cultures with that of its parent strain and an apathogenic s1133 virus. in vivo studies were initiated to determine the ts mutant's pathogenicity for day-old chicks, safety and protective levels for 6-day-old chicks, and interference with marek's disease, newcastle, and infectious bronchit ...19846091605
sequencing of coronavirus ibv genomic rna: a 195-base open reading frame encoded by mrna b.dna sequencing of genomic cdna clones of avian infectious bronchitis virus (ibv) has been carried out. 770 bases have been determined which include genomic sequences spanning the 5' termini of the two smallest mrnas of the 3'-coterminal "nested" set: mrna a and mrna b. this region contains the complete coding sequences for mrna b which are additional to those present in mrna a. two open reading frames are present, predicting proteins of mrs 7500 and 9500.19846092234
influence of newcastle disease and infectious bronchitis live virus vaccines on immune response against infectious laryngotracheitis live virus vaccine in chickens. 19846092761
monoclonal antibodies to the s1 spike and membrane proteins of avian infectious bronchitis coronavirus strain massachusetts m41.we have established four murine hybridoma cell lines which secrete specific antibody to avian infectious bronchitis virus (ibv) strain massachusetts m41. two monoclonal antibodies reacted with the spike protein and two reacted with the membrane protein. the specificity of the monoclonal antibodies for the external structural proteins was detected by immunoprecipitations using radiolabelled virus. the reactions of the monoclonal antibodies showed that one (s1) of the two glycopolypeptides associa ...19846096499
use of egg yolk in serological tests (elisa and hi) to detect antibody to newcastle disease, infectious bronchitis, and mycoplasma gallisepticum.serum and yolks from commercial flocks and from hens exposed to newcastle disease virus (ndv), infectious bronchitis virus (ibv), and mycoplasma gallisepticum (mg) were tested for immunoglobulin g antibody by the enzyme-linked immunosorbent assay (elisa) and the hemagglutination-inhibition (hi) test. yolks prepared by chloroform extraction and low-speed centrifugation performed well in the serological tests used and were a suitable alternative to serum for antibody determination by the elisa for ...19846098252
nephropathogenicity of infectious bronchitis virus.four strains of infectious bronchitis virus (ibv) were compared in chickens as to nephropathogenicity. two of the viruses were from the united states, one was from australia, and one from italy. each has been described as nephropathogenic. the australian strain proved to be most pathogenic and was then followed by the italian strain and, finally, the two viruses from the united states. with an increase in the age of the chickens there was an apparent decrease in pathogenic effect. all of the vir ...19846099569
replication and morphogenesis of avian coronavirus in vero cells and their inhibition by monensin.avian infectious bronchitis virus (ibv) was adapted to vero cells by serial passage. no significant inhibition of ibv replication was observed when infected vero cells were treated with alpha-amanitin or actinomycin d. in thin sections of infected cells, assembly of ibv was observed at the rough endoplasmic reticulum (rer), and mature ibv particles were located in dilated cisternae of the rer as well as in smooth cytoplasmic vesicles. in addition to typical ibv particles, enveloped particles con ...19846099655
sequence of the membrane protein gene from avian coronavirus ibv.cdna clones prepared from genomic rna of coronavirus ibv have been sequenced. the nucleotide sequence for the complete 5' region of mrna c, which is not present in mrnas a and b, has been determined. a sequence of 1224 bases is presented which contains a long open reading frame predicting a polypeptide of molecular weight 25 443. this is in agreement with the molecular weight of 23 000 reported for the unglycosylated form of the membrane polypeptide.19846099661
demonstration of 7-nm projections on human and avian coronaviruses.direct electron microscopy (em) of human coronavirus (hcv) strain oc43 and avian infectious bronchitis virus (ibv) strain f revealed particles with small granular projections about 7 nm in size in addition to the characteristic coronavirus particles with projections of 20 nm. relationship of the short fringed form to the conventional coronavirus particle is established by ordinary immune electron microscopy (iem) and immunosorbent electron microscopy (isem).19846099927
effect of different passage histories of infectious bronchitis virus on the sensitivity to inhibitors in chick serum and their removal by trypsin.sensitivity of the beaudette strain of infectious bronchitis virus (ibv) to non-antibody inhibitors in neutralization tests depended on the passage history of the virus. the chick embryo kidney cell-adapted (e71 cek11) virus was the most sensitive, but after one chick embryo (ce) passage (e71 cek11 e1), this virus showed reversion to the sensitivity of the parent virus (e71). ibv inhibitors in chicken serum could be removed by treatment with trypsin but not with phospholipase c.19846147998
the coronavirus avian infectious bronchitis virus requires the cell nucleus and host transcriptional factors. 19806158788
antigen requirements and specificity of a microplate enzyme-linked immunosorbent assay (elisa) for detecting infectious bronchitis viral antibodies in chicken serum.the conditions of a rapid, indirect-enzyme linked immunosorbent assay for infectious bronchitis virus (ibv) antibodies have been established. optimal sensitivity was obtained using 10 micrograms/ml protein concentration of the mass 41 strain purified from infected allantoic fluid. specificity was demonstrated with newcastle disease virus (ndv) antigen-antibody system. negligible crossreactions were observed. after bromelain or lipase treatment ibv had an elisa reactivity similar to untreated par ...19816165342
an immunoelectron microscopic and immunofluorescent study on the antigenic relationship between the coronavirus-like agent, cv 777, and several coronaviruses.a possible antigenic relationship between the porcine enteropathogenic coronavirus-like agent (cvla) and 6 known coronaviruses was examined by immunoelectron microscopy (iem) and by immunofluorescence (if). cvla did not show cross reactivity with infectious bronchitis virus, transmissible gastroenteritis virus (tgev), canine coronavirus (ccv) hemagglutinating encephalomyelitis virus (hev), neonatal calf diarrhea coronavirus (ncdcv) or feline infectious peritonitis virus (fipv). antigenic relatio ...19816166280
mouse hepatitis virus a59: mrna structure and genetic localization of the sequence divergence from hepatotropic strain mhv-3.the composition and structure of the mouse hepatitis virus (mhv)-specific rna in actinomycin d-treated, infected l-2 cells were studied. seven virus-specific rna species with molecular weights of 0.6 x 10(6), 0.9 x 10(6), 1.2 x 10(6), 1.5 x 10(6), 3.0 x 10(6), 4.0 x 10(6), and 5.4 x 10(6) (equivalent to the viral genome) were detected. t1 oligonucleotide fingerprinting studies suggested that the sequences of each rna species were totally included within the next large rna species. the oligonucle ...19816169842
antigenic variation of avian infectious bronchitis virus during replication in bhk-21 cells. 19826181761
interferon induction by avian infectious bronchitis virus in chickens. 19826182331
serotypes of avian infectious bronchitis virus isolates from field cases in japan.eight etiologic agents isolated from field cases in japan were identified as isolates of infectious bronchitis virus by agar-gel diffusion, buoyant-density determination on sucrose-density centrifugation, and morphological study by electron microscope of the purified viruses. in studies of the antigenic relationships of the eight isolates and six known infectious bronchitis viruses, antigenic diversity of these viruses was recognized from the degrees of relatedness using a plaque reduction in th ...19826186239
evaluation of the hemagglutination-inhibition test for measuring the response of chickens to avian infectious bronchitis virus vaccination.an infectious bronchitis virus (ibv) hemagglutination-inhibition (hi) test was used to assay serum-antibody titers after ibv vaccination of ibv-susceptible specific-pathogen-free broilers and commercial layers. three-week-old broilers were vaccinated via eye-drop with ibv strains that represent the antigenic spectrum of commercial vaccines--holland, massachusetts 41 (41 ms), connecticut 46, florida 18288, or jmk strain--and revaccinated 3 weeks later with either the same or a heterologous strain ...19836189476
[comparative serological studies of south german field isolates of hen infectious bronchitis virus and dutch variant strains d207, d3128 and d3896 in tracheal cultures]. 19836196022
survey of respiratory diseases among chicken flocks in japan for avian infectious bronchitis by the neutralization test with embryo-adapted beaudette strain. 19836200635
use of monoclonal antibodies to assess antigenic relationships of avian infectious bronchitis virus serotypes in the united states. 19846204515
serological comparison and antigenic relationships of seven serotypes of infectious bronchitis virus using the hemagglutination-inhibition test.the antigenic relationships, antigenic spectrum, and immunogenicity of seven isolates of infectious bronchitis virus (ibv) were examined using the hemagglutination-inhibition (hi) test. because there was a discontinuity of antigenic relationships and a high degree of cross-reactivity among serotypes of ibv in cross-hemagglutination-inhibition tests, the range of antigenic spectrum used to group the serotypes with the hi test should be wider than the limits suggested by the plaque-reduction test. ...19846204631
determination of the antigenic relationships within the massachusetts (m41) type of infectious bronchitis virus using the hemagglutination-inhibition test.the antigenic relationships, antigenic spectrum, and immunogenicity of seven ibv-massachusetts-41 isolates were investigated using the hemagglutination-inhibition (hi) test. hi titers equal to 32 are considered suspicious, titers lower than 32 are considered negative, and titers higher than 32 are considered positive immune responses to infectious bronchitis virus (ibv). some isolates of massachusetts-41 ( m41 ) were capable of inducing large quantities of antibodies in chickens following inocul ...19846204632
immunomodulatory activity of isoprinosine on experimental viral infections in avian models.the immunomodulatory activity of isoprinosine treatments have been experimentally verified on chicken infected by three different viruses: newcastle disease, fowl plague and avian infectious bronchitis. in protection tests, positive variations in the mean day of death rather than in the mortality rate were found depending on the modality of treatment. a stimulatory influence on primary anti-newcastle disease virus antibody response was observed. in the avian model the isoprinosine antiviral effe ...19846207123
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