| a new genome type of human parvovirus b19 present in sera of patients with encephalopathy. | the possible involvement of human parvovirus b19 infection in encephalopathy has been reported. to determine the characteristics of b19 viruses involved in such cases, we molecularly cloned a part of the b19 dnas derived from the sera of three patients with encephalopathy (b19 strains n80, n81 and n82), following amplification by pcr. the nucleotide (nt) sequences of the cloned dnas (nt 3147-3405) were then determined. the nucleotide sequence of n80 was similar to that of the known genome type o ... | 1995 | 7595371 |
| [responsiveness of a new parvovirus b19 antigen produced in baculovirus expression system to b19-specific igg and igm antibodies in every epidemic, 1968, 1980, 1987 and 1992 in japan]. | a new recombinant parvovirus b19 antigen was tested whether it was responsive to human serum antibodies in every epidemic year of erythema infectiosum for 25 years, because wild strains of b19 parvovirus were changeable genetically. the antigen was empty particles of both b19-vp1 and vp2 produced in baculovirus expression system. specimens were 21 sera in 1968, 19 in 1980, 44 in 1987 and 33 in 1992, derived from 67 patients with erythema infectiosum, fever and/or non-specific exanthem and aplast ... | 1995 | 7602188 |
| parvovirus infection mimicking systemic lupus erythematosus. | there are striking similarities between human parvovirus b19 (hpv-b19) infection and systemic lupus erythematosus (sle): both may present with malar rash, fever, arthropathy, myalgia, cytopenia, hypocomplementemia, anti-dna, and antinuclear antibodies (ana). therefore, it is difficult at times to differentiate hpv-b19 infection from sle presentation or exacerbation. we report 4 cases of hpv-b19 infection mimicking sle and review 10 other reported cases, all of whom were women. the similarity to ... | 1995 | 7604297 |
| life-threatening human parvovirus b19 infection in immunocompetent haemophilia. | | 1995 | 7605434 |
| purification of functional recombinant yy1 expressed in sf9 insect cells. | we expressed a gene for yy1, the multifunctional mammalian transcription factor, with a recombinant baculovirus in insect cells and obtained high levels of protein in cell lysates. a simple and efficient purification method was developed. in the final step, we used dna affinity chromatography with concatermerized sequence derived from the upstream region of the b19 parvovirus p6 promoter, which has strong affinity for yy1. approximately 1.8 mg of highly purified yy1 was obtained from 10(9) sf9 c ... | 1995 | 7606165 |
| extent of sequence variability in a genomic region coding for capsid proteins of b19 parvovirus. | genomic variability in human parvovirus b19 was analysed by direct partial sequencing of different isolates collected in italy between 1989 and 1994. dna was purified from viremic serum samples and the region of viral genome coding for structural proteins was amplified by polymerase chain reaction and partially sequenced (nt. 2400-3400). data were compared to reference isolates wi (u.k., 1973) and au (u.s.a., 1982). the average relative distance between isolates was estimated at the low level of ... | 1995 | 7611882 |
| acute parvovirus b19-induced pancytopenia in the setting of methotrexate therapy for rheumatoid arthritis. | | 1995 | 7612034 |
| evaluation of five commercial tests for detection of immunoglobulin m antibodies to human parvovirus b19. | the following commercial tests for detection of immunoglobulin m antibodies to human parvovirus b19 were evaluated: ideia parvovirus b19-igm, mrl diagnostics human parvovirus b19 igm elisa, parvoscan-b19, and biotrin parvo b19 igm eia and if. a total of 203 serum specimens from patients who probably have current b19 infections or have other viral infections and sera with rheumatoid factor were investigated. between 75 and 79 of 102 serum samples from patients thought to have current b19 infectio ... | 1995 | 7615755 |
| cloning and sequencing of the simian parvovirus genome. | we recently reported the identification of a novel simian parvovirus in cynomolgus monkeys with severe anemia. we now describe the cloning and sequencing of 4986 nucleotides of the viral dna. like the human parvovirus b19, simian parvovirus encapsidates both positive and negative single-stranded dna. the positive strand contains two large open reading frames, with the left open reading frame encoding the nonstructural protein(s) and the right reading frame encoding the two capsid proteins. simia ... | 1995 | 7618269 |
| anticomplementary activity in serum samples from patients with acute parvovirus b19 infection. | of 65 serum samples submitted for diagnostic purposes which proved to be anti-complementary by complement fixation test, 49 were parvovirus b19 igm positive. forty four of the 49 serum samples were from patients with arthropathy. acute parvovirus b19 infection should be suspected when a patient has symptoms of disease of the joints and the serum is anticomplementary. | 1995 | 7629301 |
| association between human parvovirus b19 infection and arthritis. | to gain information concerning the association between parvovirus b19 infection and arthritis. | 1995 | 7632094 |
| synthetic oligonucleotide cocktails as probes for detection of human parvovirus b19. | a cocktail of 10 oligonucleotides selected at intervals along the length of the genome of human parvovirus b19 was labelled enzymically with digoxigenin and chemically with either digoxigenin (dig) or dinitrophenyl (dnp). chemical labelling was easier and more practical for the production of large quantities of probe. pools labelled with either digoxigenin or dnp could detect 10 fg of b19 dna in a dot blot reaction using an alkaline phosphatase antibody conjugate and colorimetric detection. form ... | 1995 | 7635929 |
| persistent b19 parvovirus infections in haemophilic hiv-1 infected patients. | b19 infection can be acquired by transmission with blood factors in patients with congenital bleeding disorders, requiring clotting factor concentrates. in immunodeficient patients, the failure of immunity to clear b19 virus may produce persistent infections. the presence of b19 dna in blood samples from seven haemophilic patients with concomitant hiv-1 infection was studied over a period of three-to-four years. dot blot hybridization assays with dna and rna probes were used to detect medium hig ... | 1995 | 7636495 |
| glomerulonephritis after human parvovirus infection in homozygous sickle-cell disease. | glomerulonephritis with proteinuria of sufficient degree to manifest the nephrotic syndrome followed aplastic crises induced by human parvovirus (b19) in seven patients with homozygous sickle-cell disease, within 7 days in five patients and 6-7 weeks in two. segmental proliferative glomerulonephritis was found in all four patients who underwent acute renal biopsies and focal segmental glomerulosclerosis was found in the fifth patient who had a biopsy 4 months later. one patient recovered complet ... | 1995 | 7637482 |
| thrombocytopenia accompanying early postnatal infection by human parvovirus b19. | | 1995 | 7639290 |
| association of wegener's granulomatosis with parvovirus b19 infection: comment on the concise communication by nikkari et al. | | 1995 | 7639819 |
| fetal-maternal hydrops syndrome in human parvovirus infection. | a case of maternal generalized edema with hyponatremia, hypoosmolality and secondary hyperaldosteronism was associated with pseudomolar plasma human chorionic gonadotropin (hcg) concentrations in a case of fetal and placental hydrops due to parvovirus b19 infection. digoxigenin in situ hybridization techniques were effective in demonstrating parvovirus b19 infection on fixed tissues. hydropic changes in the placenta may have massively increased the maternal plasma hcg concentration with subseque ... | 1995 | 7639945 |
| [parvovirus b19 infection: the general aspects and gestational problems]. | parvovirus b19 is a small, heat-stable, single-strained dna virus (5.5 kb), with 23 nm icosahedral capsid discovered in 1975. since its discovery, the virus has been shown to be a causative agent of erythema infectiosum (fifth disease). under specific circumstances it can cause transient aplastic crisis, chronic anemia, arthritis and fetal death. laboratory diagnosis of recent or past b19 infection usually relies on the demonstration of virus-specific igm or igg antibodies in patient's serum. th ... | 1995 | 7644092 |
| [aplastic crisis caused by parvovirus b-19. response to treatment with low doses of gammaglobulin]. | parvovirus b19 is the etiologic agent of transient aplastic crisis, an acute episode of bone marrow failure in persons with underlying hemolysis. successful administration of gammaglobulin in some chronic cases and immunocompromised patients infected with parvovirus b-19, resulting in the elimination of this virus has been reported. however little is known about the evolution of acute aplastic crisis in treated patients with chronic hemolysis nor about the minimal effective dose of immunoglobuli ... | 1995 | 7654836 |
| b19 parvovirus. | b19 parvovirus is pathogenic in man and causes a variety of clinical illnesses, among them several haematological diseases. acute infection of a host with underlying haemolysis produces transient aplastic crisis; of the midtrimester fetus, hydrops fetalis; and of an immunocompromised patient, pure red cell aplasia. the target of b19 parvovirus infection is the human erythroid progenitor cell. infection is cytotoxic due to expression of the viral nonstructural protein. the virus can be propagated ... | 1995 | 7663050 |
| modest truncation of the major capsid protein abrogates b19 parvovirus capsid formation. | in vitro studies have suggested an important role for the minor capsid protein (vp1) unique region and the junction between vp1 and the major capsid protein (vp2) in the neutralizing immune response to b19 parvovirus. we investigated the role of the nh2-terminal region of the major structural protein in capsid structure by expressing progressively more truncated versions of the vp2 gene followed by analysis using immunoblotting and electron microscopy of density gradient-purified particles. dele ... | 1995 | 7666560 |
| parvovirus b19 antibodies in patients with haemophilia a. | | 1995 | 7667846 |
| transient acquired myelodysplasia associated with parvovirus b19 infection in a patient with congenital spherocytosis. | | 1995 | 7668238 |
| parvovirus b19 and chronic arthritis--causal or casual association? | | 1995 | 7668892 |
| persistence of parvovirus b19 in synovial fluid and bone marrow. | to determine whether parvovirus b19 (b19) persists in rheumatoid arthritis (ra). | 1995 | 7668905 |
| genetic diversity in the non-structural gene of parvovirus b19 detected by single-stranded conformational polymorphism assay (sscp) and partial nucleotide sequencing. | a homologous region in the parvovirus b19 non-structural gene (b19 nt 1399-1682) was examined in 50 samples from patients with a wide variety of b19-related disease from various countries by pcr amplification, single-stranded conformational polymorphism (sscp) assay and nucleotide sequencing. five sscp types were confirmed by nucleotide sequence analysis. of a total of 6 mutations, all were silent. types 3 and 4 accounted for 92% of strains. there was no correlation between genome type and eithe ... | 1995 | 7673389 |
| persistent infection of human parvovirus b19 in a normal subject. | | 1995 | 7674787 |
| in vitro erythroid effects of human stem cell factor in a case of human immunodeficiency virus-related chronic parvovirus b19 induced anemia. | chronic b19 parvovirus infection in patients infected with human immunodeficiency virus type 1 (hiv-1) is one cause of reversible anemia in this patient group. this report describes a case of concurrent hiv-1 and b19 parvovirus infection with pure red cell aplasia in which the anemia resolved with gammaglobulin treatment. when cultured in vitro with recombinant human stem cell factor, the red blood cell precursors from this patient demonstrated increases in both number and size, suggesting that ... | 1993 | 7682213 |
| neutralizing linear epitopes of b19 parvovirus cluster in the vp1 unique and vp1-vp2 junction regions. | presentation of linear epitopes of the b19 parvovirus capsid proteins as peptides might be a useful vaccine strategy. we produced overlapping fusion proteins to span the viral capsid sequence, inoculated rabbits, and determined whether the resulting antisera contained antibodies that neutralized the ability of the virus to infect human erythroid progenitor cells. antibodies that bound to virus in an enzyme-linked immunosorbent assay were present in antisera raised against 10 of 11 peptides; stro ... | 1993 | 7684458 |
| trends in rubella and parvovirus b19 infections. | | 1993 | 7689021 |
| life-threatening infection in two children with hemoglobin s-beta-thalassemia. | life-threatening infections with streptococcus pneumoniae and parvovirus occurred in two patients with hemoglobin s-beta-thalassemia. we recommend that acute febrile illnesses in the presence of any hemoglobinopathy be considered potentially life threatening, and managed accordingly. | 1995 | 7699536 |
| immunological abnormalities in hiv-free haemophiliacs. | since some haemophiliacs manifest profound immunodeficiency with no evidence of human immunodeficiency virus type 1 (hiv) infection, we measured the circulating immune complex (cic) level in sera obtained from haemophiliacs and addressed the question of whether viral infection is associated directly or indirectly with enhanced cic production. while more than 90% of hiv-positive individuals had a high level of cic, around 60% of seronegative ones also showed cic levels comparable to those of sero ... | 1993 | 7707343 |
| estimation of serum concentration of parvovirus b19 dna by pcr in patients with chronic anaemia. | parvovirus b19 dna was detected in serum samples from 10 out of 42 patients with chronic anaemia, the majority of whom suffered from aplastic anaemia, haemolytic anaemia, pure red cell anaemia or myelodysplastic syndrome. nested pcr methods with sensitivities of 0.005-0.05 fg dna were developed. in nine patients, b19 dna could only be detected by nested pcr. conventional pcr with a sensitivity of 50 fg b19 dna could only detect b19 dna in one patient. in the majority of b19-dna-positive patients ... | 1994 | 7709074 |
| [parvovirus b19-induced arthritis/arthropathy--an important differential diagnosis of chronic polyarthritis]. | introduction/aims: the differential diagnosis of rheumatoid arthritis (ra) and parvovirus-b19-induced arthritis/arthropathy (pba) can be difficult, but is of importance because of the different therapeutic implications. the purpose is to describe characteristic features serving to differentiate between chronic pba and ra, based on 6 personal cases and the literature. methods/patients: 6 patients presenting with acute (3 cases) or chronic pba (3 cases) over the last 5 years are described. results ... | 1995 | 7709183 |
| a case of pure red cell aplasia: follow-up on different immunosuppressive regimens. | a 66-year-old patient was admitted to our hospital in january 1992 for further evaluation of severe normocytic anemia. hemoglobin (hb) was 3.5 g/dl, reticulocyte count 1%. bone marrow showed a nearly complete lack of red cell precursors, thus favoring the diagnosis of acquired pure red cell aplasia (prca). immunosuppressive therapy with prednisolone was started but had to be supplemented with azathioprine because of a further rapid decrease in hb to 3.7 g/dl after an initial transfusion of 6 u r ... | 1994 | 7711414 |
| blood group antigens as tumor markers, parasitic/bacterial/viral receptors, and their association with immunologically important proteins. | blood group antigens (bgas) are chemical moieties on the red blood cell (rbc) membrane. some bgas (e.g., a, b, h, lewis, p, i) are widely distributed throughout the body and may not be primarily erythroid antigens. statistical correlations with abo blood groups and disease have been made for years and have been highly controversial. it is not known if bgas have a biological function. there are increasing reports of bgas [e.g., le(x) (an isomer of le(a)), le(y) (an isomer of le(b)), t, tn, "a-lik ... | 1995 | 7713584 |
| combined immunocytochemistry and non-isotopic in situ hybridization for the ultrastructural investigation of human parvovirus b19 infection. | parvovirus b19 is a single-stranded dna virus with a specific tropism for human erythroid precursor cells. the virus codes for two overlapping structural (capsid) proteins and one non-structural protein which is thought to perform essential functions in viral replication, transcription and packaging. the ultrastructural localization of these proteins was achieved in cultured haemopoietic cells derived from fetal liver which had been infected in vitro and subsequently embedded in lr white acrylic ... | 1995 | 7713756 |
| igm antibody capture haemadherence test (machat) for the detection of measles specific igm. | an antibody capture haemadherence test (machat) for detecting measles-specific igm is described. the assay is based on the antibody capture principle with rhesus monkey erythrocytes as detector system in place of labelled antisera. machat was compared with a commercial indirect enzyme immunoassay (eia) for measles-specific igm using 382 sera from patients notified as measles. there was good agreement between the two tests; 106 sera were found to contain measles igm by both tests, 7 further sera ... | 1994 | 7714039 |
| chemiluminescent microwell hybridization assay for direct detection of human parvovirus b19 dna. | a method for the direct detection of human parvovirus dna in serum samples that uses a digoxigenin-labeled rna probe to hybridize with target b19 dna, followed by capture of the hybrid onto a microtiter plate wells previously coated with a second oligonucleotide probe was developed. the captured hybrid is then detected with anti-digoxigenin-alkaline phosphatase conjugate and chemiluminescent substrate and the reaction read on a scintillation counter. the relative sensitivities of the microwell a ... | 1994 | 7714057 |
| in situ hybridisation and in situ polymerase chain reaction detection of parvovirus b19 dna within cells. | modification of an in situ polymerase chain reaction (ispcr) technique is described for the detection of b19 parvovirus infection. specific amplification of b19 dna inside fixed cells was followed by hybridisation with a digoxigenin-labelled probe and then visualised by immunochemical reaction. the assay had higher sensitivity compared to direct in situ hybridisation and still allowed cellular localisation and characterisation of infected cells. this assay can be used as a confirmatory method fo ... | 1994 | 7714060 |
| [angiocentric lymphoma associated with anemia secondary to parvovirus b19 infection]. | a 50-year-old woman was admitted to our hospital in may 1993 because of papules, cervical lymphadenopathy and interstitial pneumonia. oxygen inhalation and pulse therapy of corticosteroid were started. a biopsy of the left inguinal lymphnode showed t zone enlargement and marked vascularization with polymorphous atypical lymphoblasts, consistent with angiocentric lymphoma (immunohistochemically t cell type). in situ hybridization with eber-1 did not show association of eb virus. she was subsequen ... | 1995 | 7715084 |
| persistent parvovirus b19 infection. | | 1995 | 7715366 |
| an epidemic of aplastic crisis caused by human parvovirus b19. | human parvovirus b19 has been associated with several diseases. aplastic crisis in patients with chronic hemolytic anemia, erythema infectiosum, hydrops fetalis and arthritis are among the common diseases caused by this virus infection. in the period between july, 1991, and march, 1992, 48 patients with aplastic crises were hospitalized at saudi aramco-dhahran health center, dhahran, saudi arabia. forty-six patients had homozygous sickle cell disease, one had hemoglobin h disease and one had her ... | 1995 | 7715986 |
| antibodies anti-parvovirus b19 in chronic hepatitis c virus infection. | | 1995 | 7717346 |
| human parvovirus b19 infection in healthcare workers. | this paper reports the occurrence of human parvovirus b19 infection in seven healthcare workers in an acute hospital. the clinical presentation mimicked that of rubella and the diagnosis was made by the detection of human parvovirus b19 igm antibody in blood samples taken from the affected members of staff. the paper discusses the importance of accurate diagnosis of b19 infection because this virus has been implicated in causing or exacerbating a variety of medical conditions. b19 infection may ... | 1995 | 7718826 |
| infection due to parvovirus b19 in patients infected with human immunodeficiency virus. | parvovirus b19 has been described as a cause of chronic anemia in immunosuppressed patients, including those infected with human immunodeficiency virus (hiv). in this study serological assays and the polymerase chain reaction (pcr) were used to establish the prevalence of both prior and active infection due to parvovirus b19 among a general population of 105 hiv-infected individuals (cohort i) and among 22 hiv-infected patients with anemia (cohort ii). eight individuals in cohort i (7.6%) had ig ... | 1995 | 7727646 |
| parvovirus b19-induced red blood cell aplasia complicating iron-deficiency anemia. | | 1995 | 7729105 |
| morphology and antigenicity of recombinant b19 parvovirus capsids expressed in transfected cos-7 cells. | cos-7 cells transfected with parvovirus b19-simian virus 40 (sv40) hybrid vectors have previously been shown to express b19 structural proteins. in this study the morphology and antigenicity of b19 proteins expressed in these cells were investigated. at 84 h after transfection, approximately 10% of the cos-7 cells expressed b19 antigen, and the yield was equivalent to 2 x 10(3) to 2 x 10(5) b19 particles/transfected cell. the b19 proteins self-assembled into capsids that were morphologically and ... | 1995 | 7730807 |
| [transient erythroblastopenia in early childhood]. | background: transient erythroblastopenia is rare in young children although its frequency is increasing. population and results: six infants (four boys, two girls), aged 29-41 months (mean: 33) were admitted for polar and asthenia. only one displayed infectious episode two months earlier. hemoglobin ranged from 40 to 67 g/l and reticulocyte number from 1 to 10 g/l. mean corpuscular volume was normal as was coombs' test. there was a profound erythroblastopenia in the bone marrow in five of the si ... | 1995 | 7735443 |
| erythematous exanthem associated with primary infection by human parvovirus b19. | | 1995 | 7737770 |
| albumin batches and b19 parvovirus dna. | b19 parvovirus (b19) may be transmitted iatrogenically by blood, and its prevalence in blood donations is estimated at 1 in 3,300 to 1 in 50,000. as a large number of blood donations make up the plasma pools used to produce plasma derivatives, even a virus as rare as b19 in a population of blood donors may result in the frequent contamination of plasma batches. the percentage of albumin batches containing b19 dna has never been determined. | 1995 | 7740609 |
| clinical picture and consequences of fetal parvovirus b19 infection. | | 1995 | 7742003 |
| [parvovirus b19 infection in pregnancy]. | | 1995 | 7744336 |
| [vasculitis of viral origin. pathogenesis and therapeutic implications]. | some viruses are unquestionably the cause of vasculitis, by different mechanisms: circulating immune complexes, cryoglobulinemia and/or direct infection of the blood vessel. the main viruses responsible for vasculitis are hepatitis b & c viruses, cytomegalovirus, parvovirus b19 and human immunodeficiency virus. viral vasculitis are clinically protean, most of the time similar to idiopathic vasculitis. the manifestations due to the virus itself are sometimes hidden and vasculitis may reveal the v ... | 1995 | 7745353 |
| human parvovirus b19 can infect cynomolgus monkey marrow cells in tissue culture. | the human pathogenic parvovirus b19 cannot be grown in standard tissue culture but propagates in human bone marrow, where it is cytotoxic to erythroid progenitor cells. we now show that parvovirus b19 can replicate in cynomolgus bone marrow. cynomolgus monkeys may be a suitable animal model for pathogenesis studies of parvovirus b19. | 1995 | 7745742 |
| absence of parvovirus b19 infection in chronic fatigue syndrome. | to evaluate the presence of infection with parvovirus b19 in patients with chronic fatigue syndrome (cfs) who also had rheumatologic symptoms and mild hematologic abnormalities. | 1995 | 7748220 |
| virus validation of plasma-derived products produced by pharmacia, with particular reference to immunoglobulins. | important factors to assure the safety of plasma-derived products manufactured on an industrial scale are initial screening of the source material and validation of the manufacturing process in accordance with issued eec guidelines and us [points to consider'. pharmacia's manufacturing process for immunoglobulins contains a specific virucidal step, in which lipid-enveloped viruses are effectively inactivated with a solvent/detergent (sd) combination consisting of 0.3% tri(n-butyl)phosphate and 1 ... | 1994 | 7749047 |
| blood-borne viral infections. | the selection of blood donors and the introduction of tests identifying virus-infected donors has led to a permanent increase in the safety of blood transfusion. in most european countries, there is a low risk of infection from viruses such as hepatitis b virus, hepatitis c virus and human immunodeficiency virus. examples of viruses that pose a risk to children but not adults following transfusion-transmitted infection are parvovirus b19 and cytomegalovirus. other viruses may be transmitted in t ... | 1994 | 7749048 |
| the incidence of human parvovirus b19 infection during pregnancy and its impact on perinatal outcome. | a prospective study of parvovirus b19 infection during pregnancy was conducted at a large teaching hospital in barcelona, spain. women (1610) who were < 28 weeks pregnant at enrollment were screened for parvovirus infection. the prevalence of igg positivity was 35.03% (564/1610). the incidence of acute infection during pregnancy was 3.7% (60/1610). there were five abortions among the b19-infected women but only one was caused by parvovirus, as assessed by histologic examination and polymerase ch ... | 1995 | 7751717 |
| transmission of human parvovirus b19 by plasma derived factor viii concentrates. | although clotting factor concentrates prepared from large pools of plasma are considered to be safe for enveloped viruses (hiv, hbv and hcv), these products are likely to remain at risk for non lipid enveloped viruses. the prevalence of positive igg serology for human parvovirus b19 (hpv-b19) was determined in 53 children suffering from haemophilia a, who had been treated from birth with only one brand of a highly purified factor viii concentrate prepared by ion exchange chromatography with a so ... | 1994 | 7753615 |
| [cutaneous parvovirus infections: "gloves and socks" syndrome]. | among the viral purpuric cutaneous manifestations, papular-purpuric "gloves and socks" eruptions have been lately described. the responsibility of parvovirus b19 infection has been suspected. | 1994 | 7755312 |
| parvovirus: the expanding spectrum of disease. | treatment of parvovirus infections among immunocompromised hosts using immunoglobulin has provided the clinician with a useful therapeutic tool but has also highlighted the problems concerning chronic disease states. the discovery of the human parvovirus b19 in 1975 and subsequent studies of its effects in humans have identified this virus as the causative agent of a broad spectrum of diseases. recent improvements regarding the development of sensitive pcr techniques and methods for cultivation ... | 1995 | 7756792 |
| dot-blot hybridisation assay for detection of parvovirus b19 infections using synthetic oligonucleotides. | pools of 10 synthetic oligonucleotides with sequences derived from the genome of parvovirus b19 and of 30 bases in length were made and labelled at the time of synthesis with digoxigenin (dig) or dinitrophenyl (dnp) at the 5' end. they were used in a dot-blot hybridisation assay to detect parvovirus b19 dna in sera submitted for routine virological diagnosis where parvovirus infection was suspected. detection down to 10-100 fg dna (equivalent to 10(3)-10(4) copies of parvovirus b19 genome) was o ... | 1995 | 7760862 |
| parvovirus b19-associated haemophagocytic syndrome in healthy adults. | between august 1992 and august 1994 we found seven adult patients with haemophagocytic syndrome associated with human parvovirus b19 (hpv) infection. five of the patients were previously healthy but the other two had underlying immunosuppressive disease; the former five patients recovered spontaneously without any treatment. eight cases of hpv-associated haemophagocytic syndrome have been reported; six of them were children and the remainder were adults with immunosuppressive disease or haematol ... | 1995 | 7772537 |
| the variable clinical picture of arthritis induced by human parvovirus b19. report of seven adult cases and review of the literature. | seven patients with arthritis due to infection with human parvovirus b19 are reported, and the literature association reviewed. b19 virus arthritis most frequently affects young to middle aged women and occurs predominantly during the first six months of the year. the majority of cases have oligoarthritis or polyarthritis, and the joints most often involved are the proximal interphalangeal joints and knees. of the seven patients reported, one case developed systemic lupus erythematosus, one case ... | 1995 | 7777831 |
| acute generalized livedo reticularis with myasthenialike syndrome revealing parvovirus b19 primary infection. | | 1995 | 7778942 |
| [parvovirus b19 infection and pregnancy]. | parvovirus b19 was identified in 1975. it causes infections megalerythemia in adults associated with skin eruptions and joint pain (about 50% of the adult population is immunized). the risk of contamination in case of an epidemia is high in school teachers and school personnel. in 1984, the parvovirus b19 was implicated as the cause of fetal anasarca. the risk of transplacental contamination is estimated at 33% in case of maternal infection. pregnant women with parvovirus b19 infection and confi ... | 1995 | 7782589 |
| detection of human parvovirus b19 dna by nested polymerase chain reaction. | we describe the development of a nested polymerase chain reaction (pcr) technique used to detect human parvovirus b19 dna. it was performed in a two-step reaction, first with a pair of outer primers, then with a pair of inner (ie, nested) primers. primer sequences were selected in the vp1 gene, corresponding to the capsid protein, of human parvovirus b19. to establish the nested pcr assay, the plasmid pgem-1 containing almost the entire coding sequence of a human parvovirus b19 isolate was used. ... | 1995 | 7784006 |
| parvovirus infection and generalized edema in adults. | we describe the clinical and laboratory findings of 7 adult patients with serological evidence of recent human parvovirus b19 (hpv) infection who presented with generalized edema. six of the 7 patients had household contact with children with erythema infectiosum and had flu-like symptoms before visiting hospital. the interval between the flu-like episode and the development of edema ranged from 4 to 13 days (mean 7.0). in all 7 patients, there was serological confirmation of recent hpv infectio ... | 1995 | 7787320 |
| brachial plexitis in a patient with acute parvovirus b19 infection. | | 1995 | 7788183 |
| parvovirus b19 associated monoarthritis in a 5-year-old boy. | we describe the case of a 5-year-old boy who presented with parvovirus b19 associated arthritis of the left knee lasting for 6 weeks. other features included flu-like symptoms, a mild "slapped cheek" sign, and a macular, erythematous, lace-like rash over the chest and limbs. the analysis of the synovial fluid showed a high white cell count with a predominance of polymorphonuclear cells. the characteristic features related to parvovirus b19 associated arthropathy in children are reviewed. | 1995 | 7791179 |
| parvovirus b19 induced red blood cell aplasia in a patient with hereditary pyropoikilocytosis. | | 1995 | 7795252 |
| is still's disease associated with parvovirus b19 infection? | | 1995 | 7799719 |
| management strategies for congenital infections. | information on congenital infection is continuously expanding. new diagnostic techniques are making significant contributions to the prenatal diagnosis of several fetal infections. in this review we highlight some of the most recent advances in the diagnosis and management of the most common fetal infections, those caused by cytomegalovirus, human immunodeficiency virus 1, toxoplasma, varicella-zoster virus, and parvovirus b19. | 1994 | 7799974 |
| detection of human parvovirus b19 dna pcr products by rna probe hybridization enzyme immunoassay. | we have developed an rna probe hybridization enzyme immunoassay for detection of human parvovirus b19 pcr-amplified dna. the assay is easy to perform and increases assay sensitivity without the added inconvenience and risk of false-positive results associated with nested pcr. | 1994 | 7814562 |
| haemophagocytic lymphohistiocytosis: experience at two u.k. centres. | haemophagocytic lymphohistiocytosis (hlh) is a rare disorder of inappropriate macrophage activation. both familial and sporadic forms, which may be infection-associated, are recognized. between 1985 and 1991 we treated 23 cases of hlh (12 male, 11 female). there were eight familial cases, defined by a previously affected sibling and/or history of consanguinity, age 3 d to 15 months at presentation. the age of the remaining 15 cases varied from 1 month to 9.5 years. a potential viral trigger was ... | 1994 | 7819097 |
| lack of evidence for parvovirus b19 viraemia in children with chronic neutropenia. | | 1994 | 7819117 |
| fetoneonatal hydrops from human parvovirus b19. case report. | authors report the case of a newborn who died just a few hours after the birth as a result of intrauterine parvovirus b19 infection. diagnosis of fetal hydrops was made by ultrasound examination at the 25th week of pregnancy. etiology was established on the basis of specific antibody findings in the serum of the mother, the fetus (by cordocentesis), and the neonate; b19 virus was then observed in the fetus and the neonate tissues after death using the dot-blot hybridization assay and the polymer ... | 1994 | 7823267 |
| management of fetal hydrops associated with parvovirus b19 infection. | | 1994 | 7826963 |
| petechial glove and sock syndrome caused by parvovirus b19. | the petechial glove and sock syndrome is a recently described febrile dermatosis characterized by acral edema, petechiae in a characteristic distribution, and enanthem, which has been associated in several cases with parvovirus b19 seroconversion. we report an additional case of petechial glove and sock syndrome that further corroborates the role of parvovirus b19 as a causative agent of this syndrome in adults. | 1994 | 7835062 |
| prevalence of antihuman parvovirus b19 igg antibodies in patients with refractory rheumatoid arthritis and polyarticular juvenile rheumatoid arthritis. | we investigated the prevalence of antihuman parvovirus b19 immunoglobulin g (igg) antibody in 108 japanese patients with rheumatoid arthritis (ra) and 11 patients with polyarticular juvenile rheumatoid arthritis (jra). seropositivity of anti-b19 was significantly higher in patients with refractory ra (57.6%, 38/66) compared with patients with remittent ra (19.0%, 8/42; p < 0.001) or age-matched controls (24.3%, 19/78; p < 0.001). patients with refractory polyarticular jra had a significantly hig ... | 1994 | 7839075 |
| [survey of the prevalence of parvovirus in children]. | | 1994 | 7842114 |
| parvovirus b19 infection in hiv-1 infected patients with anemia. | serum samples were analysed for igm and igg antibodies to parvovirus by elisa and for parvovirus b19 dna by polymerase chain reaction (pcr) in 69 hiv-1 infected swedish patients with anemia and in 37 hiv-1 infected subjects without anemia. in 5/69 anemic patients, parvovirus b19 dna was detected despite the lack of igm antibody activity to the virus. the detection of parvovirus b19 dna was significantly correlated to the degree of anemia in the anemic patients. in two patients who had a chronic ... | 1994 | 7843816 |
| parvovirus b19 infection in a hypogammaglobulinemic infant with neurologic disorders and anemia: successful immunoglobulin therapy. | | 1994 | 7845728 |
| [severe hematologic involvement in parvovirus b19 infection. are antithrombin iii injections the origin of the contamination?]. | | 1994 | 7855776 |
| clinical significance of human parvovirus b19 infection in pregnancy. | we present a case of human parvovirus b19 infection in a term pregnancy that resulted in rapid development of hydrops fetalis and death. clinical aspects of this virus in pregnancy and therapeutic options are discussed. | 1994 | 7857428 |
| pure red cell aplasia caused by parvovirus b19 infection in a renal transplant recipient. | | 1995 | 7859882 |
| systemic monocyte and t-cell activation in a patient with human parvovirus b19 infection. | infection with human parvovirus b19 induces a biphasic disease. the initial phase has been associated with viremia. during the second phase of the disease, a spectrum of clinical syndromes can manifest, including erythema infectiosum, perinatal complications, and symmetric arthropathy that resembles rheumatoid arthritis. although investigators have suspected that some of the second-phase symptoms are related to immune complex formation, the pathogenesis of parvovirus b19-induced clinical manifes ... | 1995 | 7861814 |
| accurate serodiagnosis of b19 parvovirus infections by measurement of igg avidity. | in an attempt to improve diagnosis of illnesses caused by parvovirus b19 and to discriminate primary from secondary infections, a protein-denaturing assay for avidity of parvovirus-specific igg antibodies was developed. the assay used three types of purified recombinant antigens: a fusion protein containing the unique portion of the structural protein vp1, entire capsids made up of the major structural protein vp2 alone, or vp2 plus vp1. the avidity assays were evaluated by testing sequential ac ... | 1995 | 7876624 |
| [parvovirus b19]. | | 1995 | 7877350 |
| [arthropathy associated with infection by parvovirus b19. description of 4 cases]. | four cases of human parvovirus infection in which the main clinical manifestation was a polyarthritis are described. four females with ages ranging from 30 to 32 years presented with acute symmetrical polyarthralgias involving hands and knees. in addition, evidence of synovitis in the ankles and tenosynovitis of the fingers was found in two and three cases respectively. half of the patients noticed an erythematous rash in the preceding days. laboratory studies were normal in all cases. antinucle ... | 1995 | 7877351 |
| serological and virological markers of human parvovirus b19 infection in sera of hemophiliacs. | it is known that parvovirus b19 (b19) is transmitted to hemophiliacs by clotting factors prepared from human plasma. however, it is not clear whether b19 is also transmitted by the more recently used inactivated clotting factor preparations. therefore, we investigated 69 hemophiliacs, mostly children, receiving only virus-inactivated clotting factors. 49 of them (71%) were b19 igg-positive and 18 of the igg-positive hemophiliacs (37%) were also b19 igm-positive. in contrast, out of 73 age-matche ... | 1994 | 7878623 |
| prolonged erythroblastopenia in thalassemia intermedia. | | 1994 | 7896381 |
| parvovirus b19 infection during pregnancy treated with high-dose intravenous gammaglobulin. | | 1995 | 7898219 |
| false positive rubeola igm tests. | | 1995 | 7898542 |
| [rheumatic manifestations of parvovirus b19 infection]. | parvoviruses are small single-strand dna viruses. human parvovirus (pv b19) was isolated in the 1970s and recognized as a pathogen in the 80s. its role was first demonstrated in acute erythroblastopenia in patients with chronic haemolytic anaemia, then in epidemic megalerythema. more recently pvb19 has been shown to be the aetiologic agent in fetal loss and chronic infection in immunodepressed patients. further attention has focused on rheumatologic manifestations, particularly in prolonged join ... | 1995 | 7899371 |
| [periarteritis nodosa and parvovirus b19 infection]. | the authors report a case of polyarteritis nodosa which coincide with serological conversion to parvovirus b19. after review of the literature, they recall the role of this virus in human pathology and also the role of different infectious agents in the pan pathogenesis. | 1993 | 7901884 |
| b19 parvovirus dna in solvent/detergent-treated anti-haemophilia concentrates. | a transfusional b19 parvovirus infection may have severe consequences in immunocompromised hosts. the presence of b19 dna was investigated with a polymerase chain reaction (pcr) assay in 30 batches of solvent/detergent-treated clotting factor concentrates (12 batches of factor viii, 16 batches of factor ix, 1 batch of factor vii, and 1 batch of ppsb). b19 dna was detected in 6 (20%) batches, including 3 factor viii and 3 factor ix concentrates. because of the frequency of b19 dna in batches of c ... | 1994 | 7904670 |
| parvovirus b19 and erosive polyarthritis. | | 1994 | 7905974 |
| parvovirus b19-induced red cell aplasia treated with plasmapheresis and immunoglobulin. | | 1994 | 7906821 |