| asymptomatic glomerulonephritis after nonstreptococcal upper respiratory infections. | two hundred forty previously healthy military personnel with nonstreptococcal upper respiratory infections were prospectively studied to define the incidence and clinicopathologic characteristics of possible virus-associated glomerulonephritis. nine patients without preceding streptococcal infection had erythrocyte casts on urinalysis and glomerulonephritis on biopsy. of these nine, four had a reduction in total hemolytic complement and five had serologic evidence of infection with adenovirus, i ... | 1979 | 227300 |
| [acute infectious gastroenteritis. etiology and its correlation with clinical manifestations and fecal mucus]. | 343 children with acute diarrhea were studied from january 1976 to september 1977. rotavirus was the agent most frequently isolated (18 per cent) followed by shigella (12 per cent) and salmonella (10 per cent). enterotoxigenic e. coli was identified in 8 per cent and invasive e. coli only in two cases. 80 per cent of isolated rotavirus fell in the neonate group and 25 per cent in the infant group. diarrhea caused by rotavirus had a short duration, fever was negligible and abundant liquid stools ... | 1979 | 227338 |
| etiologic differentiation in lower respiratory tract virus infections by indirect immunofluorescence. | | 1979 | 227647 |
| viral respiratory disease. | | 1979 | 228463 |
| chronic keratoconjunctivitis associated with ocular adenovirus infection. | adenovirus (types 3, 4, 5) was implicated as the etiologic agent in three cases of persistent keratoconjunctivitis. in two, virus was isolated 25 and 29 months after the onset of symptoms; the third patient has suffered intermittent flares of kerato-conjunctivitis since the initial isolation of virus 13 weeks after the onset of the disease. such patients may represent a previously unsuspected source of adenovirus in the population. all were given corticosteroids topically. this treatment may hav ... | 1979 | 228557 |
| [histopathological pattern of adenovirus infection in the calf]. | pneumo-enteritis is the clinical manifestation of adenovirus infection in calf. proliferative alveolitis, endobronchitis, and peribronchitis with intranuclear inclusions in the epithelial cells, histiocytes, and macrophages were histologically recorded. proliferative changes with minor pleomorphism of cells were recorded from lymph nodes and lymph follicles of spleen and intestines. the epithelial cells of the renal tubules and liver exhibited degenerative changes, while the endothelium of the c ... | 1979 | 228628 |
| an enzyme-linked immunosorbent assay for the detection of canine antibodies to canine adenoviruses. | an enzyme-linked immunosorbent assay was used to detect canine immunoglobulin g antibodies specific for infectious canine hepatitis virus and the serologically related canine adenovirus type 2. the sequential development of homologous and heterologous antibodies was measured by the enzyme-linked immunosorbent assay and serum neutralization tests in two groups of dogs which were experimentally infected with either infectious canine hepatitis virus or canine adenovirus type 2. both tests were comp ... | 1979 | 229339 |
| virus detection in monkeys with diarrhea: the association of adenoviruses with diarrhea and the possible role of rotaviruses. | to explore the role of viruses in the etiology of diarrhea in colony-reared monkeys, direct electron microscopy, the fluorescent virus precipitin test and cell culture inoculation were used to examine the stools of monkeys with and without diarrhea. the animals were predominantly rhesus with a few macaques of other species, and included infants, juveniles and adults. adenoviruses were isolated from a higher proportion of specimens from rhesus monkeys with diarrhea (73% of specimens from infants ... | 1979 | 229340 |
| [a serological study of respiratory infections in pilgrims to mecca]. | | 1978 | 229604 |
| three serologic types of adenovirus infections of owl monkeys. | from a colony of 131 owl monkeys, adenovirus was isolated 68 times from 433 oropharyngeal and rectal swab sample pairs collected over a 2-year period. a total of 63 of 68 adenovirus isolates were grouped by neutralization into 1 of 3 types designated owl monkey adenovirus types (omav ty) i, ii, and iii. the omav ty i and ty ii were identified by neutralization with antiserum to squirrel monkey adenovirus type i and adenovirus sv-11, respectively. the omav ty iii was partially neutralized by anti ... | 1979 | 229746 |
| inhibition and enhancement of avian adenovirus plaque production by heavy and light avian adenovirus-associated viral particles. | the effect of heavy and light avian adenovirus-associated viral (a-av) particles on the replication of several adenovirus serotypes was studied in chicken embryo kidney cells. there was a significant decrease (p less than 0.05) in the number and size of adenovirus-induced plaques at a-av multiplicities of infection greater than 40. enhancement of plaque production was observed when a-av multiplicities of infection were 1 to 40. there was a significant increase in the number and size of infective ... | 1979 | 229748 |
| a new intermediate adenovirus type causing conjunctivitis. | a new adenovirus of intermediate type involved in conjunctivitis has been isolated. the virus is related to adenovirus types 13 and 30 by neutralization and to types 10 and 19 by hemagglutination inhibition. since 1976 the agent has been causing sporadic cases of conjunctivitis in different parts of the netherlands. twenty-three cases have been established by virus isolation from the affected eyes. the symptoms of the disease range from epidemic keratoconjunctivitis to pharyngoconjunctival fever ... | 1979 | 229815 |
| viral antibodies in cerebrospinal fluid diseases of central nervous system. | | 1979 | 230154 |
| viruses and virus diseases of marine mammals. | poxvirus and several serotypes of calicivirus cause recognizable disease in marine mammals. pox lesions in pinnipeds are raised and proliferative and are seen most frequently after confinement in captivity. in cetaceans, a poxvirus is associated with a much more benign and chronic lesion called a "tattoo." numerous caliciviruses of differing antigenic types have been isolated from vesicular lesions and aborted fetuses of northern fur seals and california sea lions as well as from clinically norm ... | 1979 | 230170 |
| modulation of herpes simplex virus replication in adenovirus transformed cells. | the ability of herpes simplex virus 1 to replicate in cells transformed by adenovirus type 5 is strongly dependent on the origin of the cells. studies show that adenovirus transformed rat cells lose their permissiveness while cells of hamster or human origin retain their ability to replicate hsv although at a reduced level when compared to the untransformed parent cells. one line of adenovirus transformed rat cells, 107, demonstrates thermosensitive events, allowing hsv to replicate at 34 degree ... | 1979 | 230284 |
| unusual features of the oncogenicity of chicken embryo lethal orphan (celo) virus in hamsters. | | 1979 | 230532 |
| egg transmission of avian adenovirus-associated virus and celo virus during a naturally occurring infection. | both avian adenovirus-associated virus (a-av) and celo virus were isolated from the embryonating eggs of 25-week-old black sex-linked hens during a naturally occurring infection. in the first 7 days of egg collection, a-av was isolated from 10 of 43 (23.2%) embryonating eggs, and celo virus was isolated from 8 of 43 (18.6%) embryonating eggs. both viruses were isolated from six eggs. in the next 16 days of egg collection, a-av and celo virus were coisolated from 1 of 127 (0.8%) eggs; all other s ... | 1979 | 230761 |
| a seroepizootiologic study of five viruses in a swine-evaluation station. | this serologic study was done to gain information on the spread, maintenance, and effect upon performance of five porcine viruses. blood samples were taken from two groups of 8- to 11-week-old pigs from a large number of indiana swine herds in a performance-testing station 1 week after entry, 7 weeks after entry (one group only), and at slaughter. the sera were tested by indirect fluorescent antibody tests for antibodies to transmissible gastroenteritis virus (tgev), swine influenza virus (siv), ... | 1979 | 230762 |
| experimental infection of broiler and leghorn chickens with virulent and avirulent isolates of hemorrhagic enteritis virus. | the effects of virulent and avirulent isolates of hemorrhagic enteritis virus were studied in leghorn and broiler chickens. the viruses caused subclinical infections in both strains of birds. the avirulent isolate produced enlarged spleens, inclusion bodies, and antibody in all inoculated leghorns. in broilers the avirulent virus produced infection in only half of the inoculated birds. inclusion bodies were widely dispersed in the spleens of both types of birds. the virulent virus produced infec ... | 1979 | 230804 |
| enteric adenoviruses: detection, replication, and significance. | adenoviruses can be demonstrated readily in the stools of pediatric gastroenteritis patients by electron microscopy or counterimmunoelectrophoresis, but in 45% of these cases the virus will not grow in cell culture. indirect immunofluorescence microscopy can be used to detect nongrowing strains of adenovirus; these strains have a unique single-cell fluorescence pattern. hematoxylin and eosin staining reveals adenovirus-like inclusion bodies in the same distribution as fluorescent cells. pretreat ... | 1979 | 231050 |
| [trial of a hyperimmune serum against para-influenza 3, bovine adenovirus 1, infectious rhinotracheitis and mucosal disease]. | a four-valent serum was obtained by parallel hyperimmunization of cattle with live viral suspensions of parainfluenza-3, bovine adenovirus i, the virus of infectious rhinotracheitis and the mucous disease virus. the serum's neutralizing titres are between 1:1280 and 1:2560. the lasting exploitation of serum producing cattle indicates that no competition exists between the four antigens used in the process of hyperimmunization. experimental testing of the serum on calves and its application in pr ... | 1979 | 231341 |
| adenovirus dna replication in vitro: origin and direction of daughter strand synthesis. | | 1979 | 231685 |
| a field trial with a multicomponent inactivated respiratory viral vaccine. | an inactivated virus vaccine containing strains of parainfluenza type 3 (pi3), bovine adenovirus type 3, reovirus type 1, bovine virus diarrhoea (bvd) and infectious bovine rhinotracheitis (ibr) viruses was tested in a group of 58 calves reared in a semi-intensive management system. following vaccination, 1/30, 14/30 and 17/30, showed significant rises in antibody titre to reovirus type 1, adenovirus type 3 and ibr respectively. none of the animals showed significant serological response to pi 3 ... | 1979 | 232326 |
| viral intestinal infections of animals and man. | | 1979 | 232863 |
| isolation and characterization of an adenovirus and isolation of its adenovirus-associated virus in cell culture from foals with respiratory tract disease. | an adenovirus was isolated from a foal with respiratory tract disease. the virus produced cytopathic effects (cpe) in equine embryo kidney (eek) cell culture, contained deoxyribonucleic acid (dna), was resistant to chloroform and ph 3, and was moderately resistant to heat. the virus caused hemagglutination of human (type o) erythrocytes. viral density was 1.34 g/cm,3 and diameter was 75 nm. an adenovirus-associated virus (aav) isolated from the infected cell culture was 22 nm in diameter. these ... | 1975 | 234705 |
| adenovirus deoxyribonucleic acid replication. ii. synthesis of viral deoxyribonucleic acid in vitro by a nuclear membrane fraction from infected kb cells. | a cell-free system for the study of viral dna replications was developed by the isolation of a nuclear membrane fraction "dna replication complex" from adenovirus 2-infected human kb cells late after infection. this complex which possesses both dna polymerase activity and a virus-specific endonuclease synthesizes exclusively virus-specific dna sequences in vitro by a semiconservative mechanism. analysis by rate zonal sedimentation in alkaline sucrose gradients showed that the products of the rea ... | 1975 | 235546 |
| [physico-chemical properties of celo virus strains isolated in bulgaria]. | studied were some physico-chemical properties of four celo virus strains isolated in bulgaria. their behaviour to ether, chloroform and 25 per cent trypsin, and their state at 56 degrees c and at ph 3, 0 were determined through their infectious titer as obtained on 9--10-day old chicken embryos. it was found that the size of the investigated viruses is within the range of 50 to 100 mmu, they belong to the dna-possessing group, are resistant at 56 degrees c and at ph equals 3,0. they are not sens ... | 1975 | 236618 |
| the effect of purine and pyrimidine analogues and virazole on adenovirus replication. | the multiplication of adenovirus 19 in hela cells was inhibited by various purine and pyrimidine analogues and by virazole. the formation of infectious virus and of capsid proteins (haemagglutin, group-specific complement-fixing antigen) was inhibited to the same degree, while the viral cytopathic effect (cpe) was not inhibited. the reversibility of the inhibition after removal of the substances was more complete for purine than for pyrimidine analogues. the inhibition was counteracted by sim ... | 1975 | 239555 |
| diagnosis of rhinovirus-infections by virological and immunofluorescent methods. | in january--may, 1971, detection of rhinoviurs infections among ambulatory patients with respiratory diseases in moscow was carried out in parallel by virological and immunofluorescent methods. rhinovirus infections were virologically confirmed in 13% out of 238 patients. by the indirect immunofluorescence technique, rhinovirus antigens of serotype 17 were detected in 2.2% out of 220 patients, of type 1b in 4.3% out of 183 patients, of type 48 in 5.6% out of 123 patients and of type 7 in 4.6% ou ... | 1975 | 241246 |
| [virus infections of the respiratory tract in childhood (author's transl)]. | comprehensive epidemiological studies revealed that every individual is suffering from a respiratory tract infection in the average 6 times a year. 97% of these are caused by viruses. identical clinical symptoms may be produced by many different agents, thus the etiology of an illness can only be identified by virus isolation and serological tests. the frequency of isolations is varying in adults and in children as well as in ambulatory and in hospitalized patients. the persistently observed sus ... | 1975 | 241719 |
| rejection of adenovirus 2-transformed cell tumors and immune responsiveness in syrian hamsters. | transplantation of adenovirus type 2-transformed cell-induced newborn tumor lines to different aged hamsters revealed that the cell-mediated host defenses responsible for tumor graft rejection matured early in the second week of life. when light microscopic examinations were performed during the course of tumor development, the primary histopathological difference between progressing tumors removed from newborn or thymectomized weanling hamsters and regressing lesions from normal weanlings was t ... | 1979 | 315273 |
| the nucleotide sequences at the termini of adenovirus-2 dna. | | 1979 | 318164 |
| eukaryotic dna polymerases. | | 1977 | 332062 |
| the relationship between age-dependent immunological competence of host and tumor growth in the hamster-bovine adenovirus type 3 system. | the relationship of aging to carcinogenesis was immunologically examined in the hamster-bovine adenovirus type 3 system. the age of animals at the time of virus inoculation influenced the tumor growth and latency period, but not the tumor incidence. the immunological competence of hamsters to sheep red blood cells became matured around 4 weeks after birth and was not affected by the infection of bovine adenovirus type 3 (bav-3). the strength of transplant immunity was dependent on the age of ani ... | 1977 | 333864 |
| helper factor(s) for growth of adeno-associated virus in cells transformed by adenovirus 12. | evidence is presented that a helper factor(s) for growth of adeno-associated virus (aav) is present in cells transformed by adenovirus type 12 (ad12). the growth of aav was observed in heterokaryons formed by fusion of human kb and ad12-transformed rodent cells by using ultraviolet-inactivated sendai virus without coinfection of cells with adenovirus. the presence of the helper factor(s) for aav growth in rat cells transformed by the ecori-c fragment or the hindiii-g fragment of ad12dna suggests ... | 1977 | 337298 |
| adenovirus dna: structure and function of a novel replicon. | | 1978 | 357012 |
| replication of ovine adenoviruses. | the replication of ovine adenoviruses shows intertypic and intratypic variations, e.g. the extracellular and intracellular virus yield of ort/111, a strain related to bovine adenovirus type 2, reached its peak 40 to 46 hr postinfection, in contrast to gy/14, a strain classified as ovine adenovirus type 1, which required 52 to 58 hr to reach the highest yield. the replication cycle was not appreciably influenced either by rolling of the tube cultures or the age (between 0 and 7 days) of the suppo ... | 1978 | 382754 |
| overview and status of in vitro transformation. | the development of standardized assay procedures has permitted the exploitation of cell culture systems as bioassay tools for the detection of chemical carcinogens. these systems fall generally into 3 classes: diploid cell strains, syrian hamster embryo cells; cell lines, mouse balb/c-3t3 and mouse c3h-10t1/2; and cells+virus, fischer rat cells infected with rauscher leukemia virus and syrian hamster embryo cells infected with adenovirus. the results accumulated to date show a good correlation b ... | 1979 | 387711 |
| purification and characterization of the phosphorylated dna-binding protein from adenovirus-type-2-infected cells. | the virus-coded 72000-mr dna-binding protein from adenovirus-type-2-infected cells has been purified to homogeneity by deae-cellulose chromatography, selective precipitation and gel filtration. the 72000-mr dna-binding protein is phosphorylated and the phosphate is covalently linked predominantly to serine. analysis of tryptic digests of the 32p-labeled 72000-mr protein showed that the phosphate residue(s) is present in only one peptide. the dna-binding fraction contains an additional non-phosp ... | 1977 | 408135 |
| [viruses as causal agents of gastroenteritis in infants and young children (author's transl)]. | electron microscopic examination of stool samples from 195 infants and young children with acute gastroenteritis revealed rota virus in 31 samples (= 16%), corona virus in 44 samples (= 23%) and adenovirus in 13 samples (= 7%). viruses were excreted for between 4 and 8 days in patients infected with rota virus and corona virus respectively. an outbreak of acute gastroenteritis in a premature baby unit with 24 cases is reported: corona virus was found in the stools of 15 infants. it is suggested ... | 1977 | 408644 |
| genetic evidence for a temperature-sensitive lesion in the adenovirus 7 region of the para genome. | it has previously been shown that the helper adenovirus (ad) present in the defective ad 7-sv40 hybrid population is temperature-sensitive (ts) for replication in human cells. this study has shown that the replication of the defective hybrid virus (para) in green monkey kidney cells is also restricted at 40.5 percent. complementation tests between the parental or transcapsidant para populations and sv40 or various ad serotypes revealed that the ts lesion is located in the ad region of the hybrid ... | 1978 | 413806 |
| infection of tracheal organ cultures with ovine adenovirus type 2. | | 1979 | 422766 |
| [etiological factors and disturbances of local immunity as the origin of recurrent respiratory infections. role of viruses]. | | 1979 | 471764 |
| isolation of adenovirus type 5 host range deletion mutants defective for transformation of rat embryo cells. | a series of adenovirus type 5 (ad5) deletion, insertion and substitution mutants, some of which are defective for transformation of rat cells, have been isolated. the mutants were selected as variants which lack the xba i endonuclease cleavage site at 4 map units on the viral chromosome. the deletions range in size from 150-2300 bp and are located between 1.5 and 10.5 map units. the mutants can be propagated in 293 cells (ad5-transformed human embryonic kidney cells), but are defective for growt ... | 1979 | 476833 |
| two- and three-dimensional hexon crystals. | soluble hexon of type 1 adenovirus was highly purified with different techniques and dialysed against 0.5 m acetate buffer. with this procedure tetrahedral crystals were produced from the soluble hexon capsomers of the virus capsid. electron microscopic observation of the crystallization process, revealed the development of dense two-dimensional "crystal sheets" following dialysis, in homogeneous hexon preparations containing single hexons. no such formations were observed so far with other type ... | 1979 | 484264 |
| computer programs for the assembly of dna sequences. | a collection of user-interactive computer programs is described which aid in the assembly of dna sequences. this is achieved by searching for the positions of overlapping common nucleotide sequences within the blocks of sequence obtained as primary data. such overlapping segments are then melded into one continuous string of nucleotides. strategies for determining the accuracy of the sequence being analyzed and reducing the error rate resulting from the manual manipulation of sequence data are d ... | 1979 | 493154 |
| protease of adenovirus type 2. subcellular localization. | the subcellular localization of the adenovirus type 2 core polypeptide specific protease activity was investigated using an in vitro assay system. the protease activity was recovered exclusively from infected cell nuclei and was insoluble, sedimenting with the membrane fraction. endogenous activity could be demonstrated in young virions which contain precursor pvii molecules. this protease activity only became sensitive to l-1-tosylamide-2-phenylethylchloromethyl ketone- or phenylmethylsulfonyl ... | 1979 | 500712 |
| differences between syrian hamster strains in natural killer cell activity induced by infection with pichinde virus. | | 1979 | 501084 |
| physicochemical properties and restriction maps of simian adenovirus type 38 dna. | the sedimentation constant of simian virus type 38 (sv-38) dna was estimated to be 31.6s. the intrinsic viscosity of dna was on average 86.5 dl/g and the length of the molecule determined by electron microscopy was 10.6 micrometer. the average mol. wt., as determined by sedimentation and viscometry, was 21.5 x 10(6), which agreed well with the value derived from the length of the molecule (21.4 x 10(6)) and with the value of 21.2 x 10(6) determined by the relative electrophoretic mobility of the ... | 1979 | 501339 |
| [fragmentation of the dna of simian adenovirus type 38 by using restriction endonucleases]. | the effect of restricting endonucleases eco r i, bgi ii and sal i on simian adenovirus type 38 (sv-38) dna was studied. bgl ii restrictase cleaves the virus dna into 4 fragments, a, b, g, and d, with molecular weights 9.3 x 10(6), 3.3 x 10(6), and 2.9 x 10(6) daltons, respectively. after restriction with eco r i and sal i sv-38 dna cleaves into 5 and 6 fragments, respectively. the molecular weights of eco r i fragments are a--8.2 x 10(6), b--6.5 x 10(6), c--4.0 x 10(6), d--1.27 x 10(6), and of s ... | 1979 | 506208 |
| host-cell reactivation of uv-irradiated adenovirus in cockayne syndrome. | measurements of the host-cell reactivation (hcr) of mutagen-treated virus provides a very sensitive tool for detecting abnormal dna repair. the best example of the utility of hcr studies in the examination of the dna-repair capacity of human cells has come from studies of cells from the uv-sensitive repair-deficient xeroderma pigmentosum (xp) patients. we have examined the hcr of uv-treated adenovirus type 5 (ad5) and type 2 (ad2) in cells from patients with cocayne syndrome (cs), another sun-se ... | 1979 | 514294 |
| neutralizing antibody to bovine adenovirus serotype 3 in healthy cattle and cattle with respiratory tract disease. | a serosurvey of healthy cattle for the presence of serum-neutralizing antibody to bovine adenovirus serotype 3 (bav-3) established the widespread prevalence of this agent in iowa cattle. of the animals studied, 19.1% (involving 157 calves that represented 40 outbreaks (herds) of respiratory tract disease) seroconverted to bav-3. this pathogen was implicated serologically as being involved in the cause of respiratory tract disease in six herds. bovine adenovirus serotype 3 was isolated from cattl ... | 1979 | 517833 |
| acute viral hepatitis in california sea lions. | acute viral hepatitis was diagnosed in five california sea lions (zalophus californianus) stranded along the los angeles coast. light microscopy revealed large nuclear inclusion bodies in hepatocytes. electron microscopy provided evidence that these inclusion bodies were composed of adenovirus-like virions. attempts to grow the virus in cell culture systems were unsuccessful. | 1979 | 521373 |
| susceptibility of ad12-transformed s (+) and s (-) mouse cells to cell-mediated immunity in vitro. | adenovirus type 12 (ad12)-transformed mouse cells were examined for their susceptibility to cell-mediated immunity in vitro, with respect to the activity of the virus-specific surface (s) antigen in the cells. a transformed cell line, c57at1, was established from embryonic cells of c57bl/6 mice by ad12 infection. in fluorescent antibody tests, the transformed cells were positive for the s antigen when the cells were maintained as cultures, whereas when the cells were grown as tumors in animals t ... | 1979 | 530102 |
| electron-microscopic study of the development of an equine adenovirus in cultured fetal equine kidney cells. | sequential changes induced by an equine adenovirus in cultured fetal equine kidney cells were studied by electron microscopy. the first morphological change was the appearance of type i inclusions. these inclusions developed to type ii inclusions which appeared as ring forms. type iii inclusions were formed within the central part of type ii inclusions and finally filled up most of the nuclear space. as the infection proceeded, type iv inclusions which appeared as dense dark-staining spheres wer ... | 1977 | 559531 |
| biological and biophysical characteristics of mouse adenovirus, strain fl. | mouse adenovirus, strain fl (mav), is best propagated in primary rather than secondary cultures of mouse kidney cells. virus release from the cells into the medium is fairly efficient; the average yield per cell is about 1000 tcid50. morphological and other biophysical characteristics are those of a typical adenovirus: the icosahedral shell with a diameter of 74 nm, fiber projections of 29 nm length, its localization inside the infected kidney cells, a buoyant density in csc1 of 1.34 g/ml, inhib ... | 1977 | 560839 |
| mouse adenovirus: growth of plaque-purified fl virus in cell lines and characterization of viral dna. | | 1977 | 561475 |
| biochemical studies on bovine adenovirus type 3. iii. cleavage maps of viral dna by restriction endoncleases ecori, bamhi, and hindiii. | cleavage of bovine adenovirus type 3 (bav3) dna by restriction endonucleases ecori, bamhi, and hindiii yielded 7 (a to g), 5 (a to e), and 12 (a to l) fragments, respectively. the order of these fragments has been determined to be gdacbfe for ecori fragments, aebdc for bamhi fragments, and jebkacdhfgil for hindiii fragments, and cleavage sites of these enzymes have been mapped on the genome of bav3. bav3 preparation contains incomplete virus whose genome has a deletion of about 13% of complete v ... | 1978 | 568182 |
| [production and characteristics of seven rat embryo cell lines transformed by adenovirus type 5 and its dna]. | seven cell lines transformed by adenovirus type 5 and its dna were obtained. it was shown that different cell lines contain the fragments of viral dna which differ in length and number of copies per dna of diploid cells. they contain from the left end 6% of the viral dna to complete or almost complete viral genome. all studied cell lines were sensitive to reinfection with adenovirus type 5. they produced no virus being cocultivated with cell sensitive to the virus. no cell line was able to induc ... | 1979 | 571524 |
| comparative studies on the soluble proteins of adenovirus type 1. | the soluble proteins of adenovirus type 1 have been separated and purified. their antigenic characteristics were compared in different precipitation experiments performed in electric field. both two-dimension immune electrophoresis and rocket electrophoresis can successfully be applied for quick diagnostic purposes. quantitative determination of virus proteins is also feasible by rocket electrophoresis. the isoelectric point values of hexon, penton and fibre were pi 4.55, pi 4.69 and pi 7.07, re ... | 1977 | 602822 |
| erythrocyte acetylcholinesterase activity is not affected by adenovirus-induced haemagglutination. | no correlation was found between adenovirus-induced haemagglutination and ache activity of human rbcs as ascertained with three different types of virus. the haemagglutination titre of the adenoviruses did not decrease when rbcs with ache inhibited by dichlorvos or eserine were used. the receptors involved in active haemagglutination by adenoviruses thus seem to differ from those acting in passive haemagglutination. the ache inactivating effect of substances such as tannic acid and glutaraldehyd ... | 1977 | 617949 |
| transcription of adenovirus-associated virus rna in isolated kb cell nuclei. | | 1978 | 619486 |
| characteristics of hamster cells transformed by the combined action of chemical and virus. | pretreatment of hamster cells with chemical carcinogen enhances transformation by a simian adenovirus, sa7. such transformants were compared to cells transformed by sa7 alone for the presence of virus-specified antigens, and for the ability to clone in soft agar and to form progressively growing sarcomas in hamsters. the sa7 "t" antigen content and the relative cloning efficiencies in agar of cell lines of both groups were similar. some cell lines obtained by pretreatment with chemical carcinoge ... | 1978 | 624597 |
| structural relationships of low molecular weight viral rnas synthesized by rna polymerase iii in nuclei from adenovirus 2-infected cells. | previous studies have shown that endogenous class iii rna polymerase(s) in nuclei from adenovirus 2-infected cells synthesize virus-coded rna species which are approximately 200 (v200), 156 (v156), and 140 (v140) nucleotides in length (weinmann, r., brendler, t. g., raskas, h.j., and roeder, r. g. (1976) cell 7, 557-566). the v156 nuclear rna is identical in sequence to the major virus-associated rna (va rna1 or 5.5 s rna) synthesized in intact cells (ohe, k., and weissman, s. m. (1971) j. biol. ... | 1978 | 659408 |
| [viruses--causes of human tumor forms]. | | 1978 | 683122 |
| localization of simian adenovirus 7 (sa 7) transcription and replication in lytic infection. an ultracytochemical and autoradiographical study. | we have studied sa7 (simian adenovirus 7) lytic infection at the ultrastruct level. the use of cytochemical techniques which specifically stain dna or preferentially stain ribonucleoproteins permitted the analysis of the structure of the virus-induced nuclear inclusions, and revealed presumed virus dna before the appearance of other nuclear alterations. correlation of these findings with high resolution autoradiography enabled the functions of virus dna replication and transcription to be ascrib ... | 1978 | 690607 |
| morphological revertants of adenovirus type 12-transformed hamster cells. | morphological revertants have been isolated from one line of adenovirus type 12-transformed hamster cells. this line, t637, is oncogenic in hamsters and contains multiple copies of the virus genome per cell. different parts of the virus genome are represented in non-stoichiometric amounts and the virus dna persists in the cells in an integrated form. the pattern of integrated virus genomes has been determined by the blotting technique. in the t637 line, morphological revertants arise spontaneous ... | 1978 | 690612 |
| [associative interaction between mycoplasma pneumoniae, influenza virus and adenovirus in an organ culture of adult human lung]. | | 1978 | 696091 |
| combined antiviral effect of dna inhibitors on adenovirus multiplication. | 9 purine or pyrimidine analogues or other inhibitors of dna synthesis were studied on their single and combined inhibitory effect on the multiplication of adenovirus 9--15 in hela cell cultures. the criterium of inhibition was the total suppression of the formation of infectious virus. inhibiting concentrations were derived from isobolograms. 34 of 36 drug pairs acted in a synergistic manner, one pair showed additive and one no interaction. | 1978 | 699675 |
| detection and quantitation of adenovirus type 12 transforming dna segments and their application in etiological studies of human neoplasia. | the oncogenic capacity of the tumour virus lies in a small region of the viral genome, and tumour cells and cells transformed by dna tumour viruses often contain only a part of the viral genome, the portion including the transforming gene(s). the detection and quantitation of these gene(s) in tumour cell dna can provide evidence for or against a possible etiological role of the virus in human neoplasia. in this communication, the isolation, purification and identification of the transforming seg ... | 1978 | 730198 |
| [density gradient and ultrastructure of bovine adenovirus type 1]. | isolated and purified were virions and hexones from the bovine adenovirus type 1. the infectious virions were of equilibrium density of 1.34 g/cm3, and the empty capsids--of 1.305 g/cm3. the virions had a facet length of 43.5 nm, their size being 82.7 nm (distance between opposite poles) to 74.0 nm (diameter of the virion). the fibers of the bovine adenovirus type 1 measured 26.0--28.0 nm. the virus was destroyed by 30 per cent formamide, yielding free hexones of 1.290 g/cm3 of equlibrium densit ... | 1978 | 741648 |
| isolation of bovine adenovirus type 1 without an adenovirus-associated virus. | this report describes the isolation and identification of a strain of bovine adenovirus type 1 from a cow in a herd that had undergone an episode of diarrhea and abortions. this strain of bovine adenovirus type 1 did not agglutinate either guinea pig or human 0 erythrocytes, and no 20-nm virus particles were evident by electron microscopy, further indicating that it was not contaminated by adenovirus-associated virus. | 1978 | 749583 |
| genetics of adenoviruses. | | 1976 | 775934 |
| demonstration of adenovirus associated endonuclease. | in hep-2 and amnion cell cultures infected with type 1 adenovirus the dnase activity of cell extracts was measured on 32p-labelled escherichia coli dna substrate. enzyme activity was demonstrated by the acid soluble nucleotides released from the 32p-dna and by the decreased sedimentation rate of labelled dna. high dnase activity was measured in both adenovirus infected and in untreated hep-2 cell extracts. nuclease activity of the amnion host cells being much lower than that of hep-2 cells, viru ... | 1976 | 779403 |
| [hexokinase activity in the soluble fraction of the nucleus and cytoplasm in cell cultures infected with human adenoviruses types 6 and 12]. | a study was made of the activity of hexokinase in the soluble fraction of the nucleus and cytoplasm of fibroblasts of rat embryos (primary cultures) during contact with infectious (a6) and oncogenic (a12) human adenoviruses. a high activity of the enzyme was seen in the cytoplasmic fraction in comparison with the nuclear one both in the infectious adrenovirus of type 6 this value differed from the normal and in the experimental samples. with the action of the normal but insignificantly. marked c ... | 1976 | 779866 |
| [molecular biological bases for the tumorigenic action of viruses]. | | 1976 | 781480 |
| [cell transformation and adenovirus oncogen]. | | 1976 | 799871 |
| propagation in e. coli of bacteriophage lambda with integrated fragments of adenovirus 2 dna. | hybrid genomes of bacteriophage lambda with integrated fragments of adenovirus type 2 (ad2) dna have been constructed in vitro and propagated in e. coli. dna from a derivative of bacteriophage lambdaplac5 (rambach and tiollais, 1974) was used as a vector. the two fragments of the vector dna contain all the essential genes for the replication of the lambda dna but are too short to be encapsidated. insertion of dna is therefore essential for plaque formation which constitutes a selection method fo ... | 1976 | 802391 |
| detection and serological identification of adeno-associated virus in avian adenovirus stocks. | eleven avian adenovirus strains were tested for the presence of avian adeno-associated viruses (aaav). six strains contained aaav. electron microscopy using rabbit anti-aaav serum was useful in detecting the satellite virus. the aaav previously isolated from guail bronchitis virus was related to each of the six new isolates by immunoagglutination, complement fixation, immunodiffusion, and neutralization tests. | 1975 | 803468 |
| tumor-specific transplantation antigen(s) of bovine adenoviruses. | protection against bovine adenovirus type 3-induced primary or transplantable tumors was studied in hamsters immunized with bovine adenoviruses, human adenovirus type 12 (a-12), simian adenovirus type 7 (sa7), or chicken-embryo-lethal-orphan (celo) virus. newborn hamsters inoculated with 2.3 times 10-5 plaqueforming units of bovine adenovirus type 3 were given injections of bovine serotypes 1, 2, or 3 during the latent period of tumor development. no hamsters immunized with type 3 and only 47% o ... | 1975 | 803562 |
| hemorrhagic enteritis of turkeys: electron microscopy of the causal virus. | spleens of turkey poults inoculated with hemorrhagic enteritis virus were examined by electron microscopy. negatively stained infectious extracts of spleen tissue contained adenolike virions averaging 75-80 nm in diameter. intranuclear virions, either scattered or in crystalline arrays, were observed in thin sections of spleen tissue four days after inoculation. the chromatin was condensed and marginated in the nuclei of most cells which contained virions. some virions in sections appeared as ho ... | 1975 | 804306 |
| antigenic characterization of the inclusion body hepatitis virus. | proof that inclusion body hepatitis of chickens (ibhc) is caused by a virus (ibhv) was established in 1973. characterization of ibhv has been conducted by employing the standard criteria for virus classification. the results have indicated that ibhv is a member of the avian adenovirus group. however, no antigenic relationship was found between ibhv and two other avian adenovirus isolates (celo and indiana c) as observed by hemagglutination (ha) and virus-serum neutralization tests. another adeno ... | 1975 | 804835 |
| bovine adenoviruses. a review of vaccination experiments. | the adenoviruses are one of the virus groups involved in the respiratory disease complex in young cattle and are one of the causes of great economic losses. a statement of the identified bovine adenoviruses and their pathogenicity in cattle is given. the number of eight bovine serotypes is expected to increase as research continues and unidentified strains are compared with recognized types. the vaccination trials which have been done until now with bovine adenovirus vaccines have been limited t ... | 1975 | 805073 |
| biological activation of virus-like particles from chemically induced guinea pig sarcomas. | | 1975 | 806925 |
| electron microscopy of adenovirus cores. | adenovirus type 5 'cores' prepared by heating in the presence of deoxycholate and partially purified on a glycerol density gradient could be visualized as roughly isometrical particles with a condensed centre from which twisted filaments or loops of dna emanated. this compact structure was readily dispersed by spreading on distilled water or by treatment with edta, nonidet, dnase or trypsin. spreading with nonidet was particularly effective in unfolding the cores and revealing long filaments abo ... | 1975 | 808587 |
| significance of treatment interval and dna repair in the enhancement of viral transformation by chemical carcinogens and mutagens. | treatment of syrian hamster embryo cells with diverse classes of chemical carcinogens enhanced transformation by a carcinogenic simian adenovirus, sa7. optimal enhancement was a function of time of chemical addition in relation to time of virus addition and cell transfer. aflatoxin b1 (afb1) and the polycyclic hydorcarbons, benzo(a)pyrene (b(a)p), 3-methylcholanthrene (mca), and 7,12-dimethylbenz(a)anthracene (dmba) enhanced sa7 transformation when added prior to virus, but inhibited transformat ... | 1976 | 816474 |
| [transfer of adenovirus dna into the nucleus at the beginning of infection of hela cells]. | 2 hrs after infection of hela cells with ad 7, viral dna was found in the nuclei. inactivation of the virus with ultraviolet or antiserum, or pretreatment of the cells with chloroquine, did not affect the localisation of the dna. the intranuclear dna originated at least partly from viral particles passing through lysosomes. the appearance of ad 7 dna in the nucleus was delayed as compared to ad 5 dna. | 1976 | 816550 |
| [scanning electron microscopic studies of viruses and viral infections]. | | 1976 | 824477 |
| diagnosis of enteritis virus. | | 1976 | 824651 |
| nuclear matrix of hela s3 cells. polypeptide composition during adenovirus infection and in phases of the cell cycle. | a subnuclear fraction has been isolated from hela s3 nuclei after treatment with high salt buffer, deoxyribonuclease, and dithiothreitol. this fraction retains the approximate size and shape of nuclei and resembles the nuclear matrix recently isolated from rat liver nuclei. ultrastructural and biochemical analyses indicate that this structure consists of nonmembranous elements as well as some membranous elements. its chemical composition is 87% protein, 12% phospholipid, 1% dna, and 0.1% rna by ... | 1977 | 830654 |
| the fate of type 7 adenovirions in lysosomes of hela cells. | properties of type 7 adenovirions in lysosomes of hela cells were studied 12 hr postinfection. viral particles were transferred to the lysosomes very quickly after initiation of penetration, i.e., after 10 min of incubation at 37 degrees. no morphological modification of the virion was detected for 6 hr postinfection. however, by 12 hr postinfection, the virion was no longer recognizable. most of the virus remained infectious for 2 hr, whereas after 12 hr the infectivity was abolished. soon afte ... | 1977 | 841871 |
| febrile neonatal illness associated with echo virus type 5 in the cerebrospinal fluid. | | 1977 | 844242 |
| immune complex-induced platelet aggregation and 3h-serotonin release: microtechnique for an assay of virus and mycoplasma antigens or antibodies. | human platelets are very sensitive indicators of immune complexes. the microplate test presented employs aggregation and/or release reaction of platelets as parameters of an antibody and antigen assay. the measurement of the release reaction is approximately 5 times more sensitive than the examination of aggregation patterns. antibody titres of patients' sera attained values of 1/780,000 for adenoviruses and mycoplasma pneumoniae. the determinations of 3h-serotonin release quantitative and there ... | 1977 | 864263 |
| studies on the different properties of adenovirus penton. | the physical, chemical, immunological and biological properties of the soluble penton antigen of adenovirus strains types 1, 5, 7, 8 and 9, belonging to three distinct subgroups of human adenoviruses, were analysed. mono-, di-, and polymers of the penton were studied by complete and incomplete hemagglutination and a method was developed to separate pentons of subgroup ii. immunological techniques were developed for the rapid detection of penton antigen. elution characteristics of the pentons of ... | 1977 | 867802 |
| [importance of the lysosomes in the reproduction of adenovirus and coxsackie b-6 virus in cell culture]. | | 1977 | 895575 |
| molecular similarities among the adenovirus-associated virus polypeptides and evidence for a precursor protein. | | 1977 | 898671 |
| [isolation of viruses from urban sewage: comparison of methods]. | a comparison has been made among some methods (concentration on aluminium hydroxide precipitates, polymer two-phase separation, cellulose membrane filtration, direct inoculation) for virus isolation from raw sewage. the concentration on aluminium hydroxide has given significantly better results than the other methods. no significant differences have been noted between the two-phase separation and direct inoculation method. the membrane filtration method has given no positive result. all isolated ... | 1977 | 901633 |
| enzymatic properties of viral replication complexes isolated from adenovirus type 2-infected hela cell nuclei. | when hela cell nuclei, isolated 17 h after infection with adenovirus type 2 (ad2), were extracted with 200 mm ammonium sulfate, ad2 nucleoprotein complexes were selectively released. these complexes contained a dna polymerase activity that corresponded to dna polymerase molecules actively engaged in ad2 dna replication. under our high-salt (200 mm ammonium sulfate) incubation conditions, where no reinitiation occurred, full-length ad2 dna chains were synthesized by elongation of chains that had ... | 1977 | 916022 |
| isolation of the viral dna replication complex from adeno-associated virus type 1-infected cells. | the replication complex active in adeno-associated virus type 1 (aav-1) dna synthesis in vitro was solubilized, with a nonionic detergent, from the nuclei of human embryonic kidney cells coinfected with aav-1 and an early temperative-sensitive mutant (ts125) of human adenovirus type 5 at the nonpermissive temperature (40.5 degrees c). the complex sedimented with a mean size of 23s and contained parental aav-1 dna. most of the dna synthesized with the aav-1 dna replication complex in vitro was aa ... | 1977 | 916024 |
| [morphology of bovine adenovirus type i]. | electron-microscopic studies were carried out on a concentrated and purified adenovirus type i. it was found that its morphologic aspects were characteristic of the adenovirus group. in the preparations of this virus there were large amounts of incomplete virions and soluble components--hexons, groups of hexons and pentons. some of the virions were grouped in p-crystal structures or were released as grouped in cell vacuoles. the hexons presented a six-angle contour and a central cavity. the pent ... | 1977 | 929960 |