| induction of virus specific and h-2 restricted cytotoxic t cells by uv inactivated murine cytomegalovirus. | infection of c3h mice with live or uv-inactivated murine cytomegalovirus (mcmv) was able to generate population(s) of lymphocytes in the spleen (ctl) which could exert a lytic effect against l cells infected with mcmv but not against uninfected or those infected with hsv-1. the effector cells proved to be theta-bearing t cells and the lysis of target cells was h-2 restricted. data presented show that early viral protein synthesis but not viral dna synthesis was necessary for the appearance of re ... | 1979 | 228635 |
| specific cell-mediated immunity in children with congenital and neonatal cytomegalovirus infection and their mothers. | cell-mediated immunity (cmi) to cytomegalovirus (cmv) and herpes simplex virus type 1 (hsv-1) was examined in 35 mothers and 30 of their offspring with congenital or neonatal cmv infection by means of the lymphocyte transformation assay. eleven offspring did not respond to cmv antigen, and 15 of the 19 positive children displayed lower responses than those of normal immune adults. productive infection in the younger children at the time of assay and the presence of disease correlated strongly wi ... | 1979 | 229171 |
| ocular infection with herpes simplex virus type 1: prevention of acute herpetic encephalitis by systemic administration of virus-specific antibody. | the effects of virus-specific antibody on the pathogenesis of infection due to herpes simplex virus type 1 (hsv-1) following corneal inoculation of young adult mice were studied. hsv-1 was inoculated onto the corneal surface immediately after trephination with a capillary pipette. after inoculation, virus spread to the trigeminal ganglion and brain within two days and caused acute herpetic encephalitis, which killed infected animals eight to 10 days after infection. rabbit hyperimmune antisera t ... | 1979 | 229174 |
| structural analysis of precursor and product forms of type-common envelope glycoprotein d (cp-1 antigen) of herpes simplex virus type 1. | the type-common cp-1 antigen of herpes simplex virus type 1 (hsv-1) is associated in the infected cell with two components, a 52,000-molecular-weight glycoprotein (gp52 or pd) and a 59,000-molecular-weight glycoprotein (gp59 or d). the larger form (d) is also found in the virion envelope. it was postulated that pd is a precursor of d. we found that pd shared methionine and arginine tryptic peptides with d isolated from infected cell extracts. d isolated from infected extracts had the same trypri ... | 1979 | 229243 |
| gene expression of herpes simplex virus. i. analysis of cytoplasmic rnas in infected xeroderma pigmentosum cells. | rnas which are synthesized and accumulate in the cytoplasm of uninfected and herpes simplex virus type 1 (hsv-1)-infected xeroderma pigmentosum (xp) cells in the presence of cycloheximide (early rnas) or absence of drugs (late rnas) were analyzed by electrophoresis through denaturing polyacrylamide gradient slab gels. hsv rnas were selected by hybridization ot hsv dna covalently bound to cellulose. no hsv-specific low-molecular-weight (4s to 10s) rnas were detected. however, several changes were ... | 1979 | 229249 |
| regulation of persistent infection with herpes simplex virus in vitro by hydrocortisone. | about 1% of raji cells showed sensitivity to herpes simplex virus type 2 (hsv-2) infection when tested by infectious center assays or immunofluorescence tests, and the percentage did not change during cell passage. the addition of hydrocortisone to raji cells persistently infected with hsv-2 caused a marked increase in virus production and in the number of hsv-producing cells. in the case of hsv-1, it was shown that the addition of hydrocortisone was required to maintain persistent infection. th ... | 1979 | 229255 |
| herpes simplex virus dna synthesis in a partially purified soluble extract from infected cells. | hep-2 cells were infected with herpes simplex virus type 1 and extracted with 0.25% triton x-100 and 0.1 m nacl. the extract was sedimented on sucrose gradients, and the fractions containing the endogenous dna polymerizing activity (replication complex) were collected. the properties of the replication complex were partially characterized. under optimal conditions 375 pmol of dtmp per micrograms of dna was incorporated, which corresponds to about 50% replication of preexisting viral dna. the rep ... | 1979 | 229258 |
| alterations in glycosphingolipid patterns in a line of african green monkey kidney cells infected with herpesvirus. | the major glycosphingolipids (gsls) of a line of african green monkey kidney cells (bgm) were characterized as glucosylceramide, lactosylceramide, galactosyl-galactosyl-glucosylceramide, and n-acetylgalactosaminyl-galactosyl-galactosyl-glucosylceramide. neutral gsls accounted for approximately 80% of the total gsls isolated. the predominant gangliosides were n-acetylneuraminyl-galactosyl-glucosylceramide, n-acetylgalactosaminyl-n-acetylneuraminyl-galactosyl- glucosylceramide, and galactosyl-n-ac ... | 1979 | 229262 |
| herpes simplex virus glycoproteins: participation of individual herpes simplex virus type 1 glycoprotein antigens in immunocytolysis and their correlation with previously identified glycopolypeptides. | tissue culture cells infected with herpes simplex type 1 virus express virus-specified glycoprotein antigens on the plasma membrane. three of these have been previously identified and have been designated as ag-11, ag-8, and ag-6. in the present study, immunoglobulins to each of the antigens were shown to be capable of mediating immunocytolysis in the presence of either complement (antibody-dependent complement-mediated cytotoxicity) or peripheral blood mononuclear cells (antibody-dependent cell ... | 1979 | 229263 |
| membrane proteins specified by herpes simplex viruses. v. identification of an fc-binding glycoprotein. | a glycoprotein with affinity for the fc region of immunoglobulin was isolated from extracts of cultured cells infected with herpes simplex virus type 1, and experiments were done to characterize its properties and to investigate whether it could account for the fc-binding activity previously demonstrated on the surfaces of intact herpes simplex virus-infected cells. the technique of affinity chromatography was used to identify and isolate the fc-binding glycoprotein and to demonstrate the specif ... | 1979 | 229267 |
| immunogenicity of subviral herpes simplex virus preparations. i. formation of neutralizing antibodies in different animal species after administration of herpes simplex virus solubilized antigens. | production of neutralizing antibodies was followed in guinea pigs, rabbits, hamsters and mice immunized with crude antigen extracts (am) from human diploid cells infected with herpes simplex virus type 1. the am induced relatively high levels of neutralizing antibodies in all four species. the antibodies were predominantly complement-requiring and remained so even after administration of repeated am doses. with the strains used, the antibody response was predominantly type specific and, surprisi ... | 1979 | 229789 |
| herpes simplex virus dna polymerase, thymidine kinase and deoxyribonuclease activities in cells infected with wild type, ultraviolet-irradiated and defective virus. | the dna polymerase, thymidine kinase and deoxyribonuclease activities were studied in cells infected with wild type (wt), ultraviolet (uv)-irradiated and defective herpes simplex virus type 1. all three enzymatic activities were expressed in cells infected with wt virus. in cells infected with uv-irradiated virus, the thymidine kinase and deoxyribonuclease activities were inhibited and the dna polymerase activity was markedly suppressed. in cells producing defective virus, there was thymidine ki ... | 1979 | 229801 |
| isolation of the major herpes simplex virus type 1 (hsv-1)-specific glycoprotein by hydroxylapatite chromatography and its use in enzyme-linked immunosorbent assay for titration of human hsv-1-specific antibodies. | a 131,000 molecular weight herpes simplex virus type 1 (hsv-1) glycoprotein designated antigen number 6 (ag-6) was previously shown to possess almost exclusively hsv-1-specific antigenic sites. fused rocket and crossed immunoelectrophoresis of fractions obtained from hydroxylapatite chromatography of crude hsv-1 antigen (triton x-100-solubilized, infected tissue culture cells) showed that a subfraction of ag-6 could be separated from the other hsv antigens. enzyme-linked immunosorbent assay with ... | 1979 | 230199 |
| modulation of herpes simplex virus replication in adenovirus transformed cells. | the ability of herpes simplex virus 1 to replicate in cells transformed by adenovirus type 5 is strongly dependent on the origin of the cells. studies show that adenovirus transformed rat cells lose their permissiveness while cells of hamster or human origin retain their ability to replicate hsv although at a reduced level when compared to the untransformed parent cells. one line of adenovirus transformed rat cells, 107, demonstrates thermosensitive events, allowing hsv to replicate at 34 degree ... | 1979 | 230284 |
| differences in the morphology of herpes simplex virus infected cells: i. comparative scanning and transmission electron microscopic studies on hsv-1 infected hep-2 and chick embryo fibroblast cells. | infection with herpes simplex virus type 1 (hsv-1) induces different morphological changes in different cell lines. this is demonstrated by comparative scanning (sem and transmission (tem) electron microscopic investigations of cell cultures prepared under identical conditions. sem of hsv-1 infected hep-2 cells reveals a slightly altered cell surface: only the number of the microvilli is reduced. large amounts of released virions are detectable adhering to the outer plasma membrane. ultra-thin s ... | 1979 | 230292 |
| [design of inactivated polyvalent antiherpes vaccines]. | the variants of inactivated vaccine against herpes simplex virus have been developed: 1, 2 and 1 + 2. the first two variants consist of 5 different strains of herpes virus of the appropriate type, and the third variant of their mixture. the strains differ in their antigenic and biological characteristics. complete inactivation of the virus suspension was achieved by treatment of the vaccine with formalin. herpes vaccines were used for immunization of 477 patients with frequently relapsing forms ... | 1979 | 230648 |
| growth inhibition by acycloguanosine of herpesviruses isolated from human infections. | inhibition by acycloguanosine (acg) of plaque formation by harpes simplex virus types 1 and 2 (hsv-1 and hsv-2), varicella-zoster virus, and cytomegalovirus was studied. seventeen clinical isolates of hsv-1 were inhibited by acg at a mean 50% inhibitory dose (id(50)) of 0.15 +/- 0.09 mum. the mean id(50) for 10 isolates of hsv-2 was 1.62 +/- 0.76 mum, and for four isolates of varicella-zoster virus it was 3.75 +/- 1.30 mum. the id(50)'s for two cytomegalovirus isolates were 100 and 160 mum, and ... | 1979 | 230783 |
| use of protein a-treated sera in unmasking herpes simplex virus type 1 (hsv-1) immunoglobulin a and identifying hsv-1 immunoglobulin g as the predominant neutralizing antibody. | treatment of human sera with protein a reduced the amounts of immunoglobulin g (igg), iga, and igm detected by radial immunodiffusion. this treatment also decreased the amount of herpes-specific igg and igm detected by radioimmunoassay, whereas it increased and even unmasked the amount of herpes-specific iga detected. comparison of protein a-treated sera with untreated sera indicated that herpes simplex virus type 1 igg was responsible for more than 92 to 99% of the serum neutralizing activity. | 1979 | 231047 |
| integration of herpes simplex virus type 1 dna into the dna of growth-arrested bhk-21 cells. | the ability of hsv-1 dna to become associated with host cell dna in an alkaline-stable form has been demonstrated following infection of a ts baby hamster kidney growth mutant (ts btn-1), at the non-permissive temperature (39.5 depgrees c). after 8 h pre-incubation at 39.5 degrees c, ts btn-1 cells infected at this temperature using m.o.i. ranging from 0.5 to 200 p.f.u./cell fail to replicate virus dna even though transport of input virus genomes to the nucleus is the same at both permissive and ... | 1979 | 231087 |
| host-cell response to herpes virus infection in central and peripheral nervous tissue in vitro. | in an organotypic nerve cell culture system, all cells in both the central and the peripheral nervous system (cns, pns) components supported replication of herpes simplex virus types 1 and 2 (hsv 1, hsv 2). in hsv 1 infection, cellular response was particularly characterized by the formation of small syncytia (which involved neurons) and by the presence of bundles of interwoven fine filaments within the nuclei of infected cells. in hsv 2 infection, groups of parallel tubules characteristically f ... | 1979 | 231090 |
| inactivation by u.v.-irradiation of interfering herpes simplex virus particles: interference requires a functional genome. | the sensitivity of interfering herpes simplex virus (hsv) particles to u.v.-irradiation was studied in a virus stock of hsv-1 strain ang that contained an excess of interfering over infectious particles. following u.v.-irradiation, samples of this virus stock were assayed for their plaque-forming capacity and their capacity to interfere with the replication of unirradiated standard virus. depending on the assay conditions, interfering particles appeared to be less, equally, or more sensitive to ... | 1979 | 231091 |
| [comparative value of temperature marker and neutralization test for subtyping of herpes simplex viruses (author's transl)]. | two methods for the differentiation of herpes simplex virus (hsv)--rct and neutralization by specific antisera--have been applied to 8 prototype strains and 105 strains isolated from various clinical cases. the evaluation of the ii/i index allows to choose the most specific rabbit antisera. the rct 40 degrees 3 c marker test gives comparable results for hsv grown in rk13 and human fibroblasts as well; it is in agreement with the immunological test in 48% of the cases. the delta tc values used to ... | 1979 | 231398 |
| comparative study of herpes group virus-induced dna polymerases. | a comparative biochemical study of virus-induced dna polymerases was made among the herpes group viruses: namely, herpes simplex virus (hsv) type 1 and type 2, human cytomegalovirus (hcmv) and varicella-zoster virus (vzv). although these virus-induced enzymes shared some biochemical properties, they differed in several important aspects. all these virus-induced dna polymerases could efficiently use poly(dc) . oligo(dg)12--18 and poly(da) . oligo(dt)12--18 as template-primers. however, in phospho ... | 1979 | 231586 |
| the effects of ultraviolet and ionizing radiation on herpesviruses, sv40 and adenoviruses in relation to the small-plaque effect. | the small-plaque effect occurs with a wide range of herpesviruses following irradiation with ultraviolet light. the 37 per cent survival (d37) values, or dose required for one lethal hit (e-1), for herpes simplex, pseudorabies and pigeon herpesviruses in different cells indicate a broad spectrum of host-cell repair capacity. other dna-containing viruses such as sv40 and adenoviruses, which also replicate in the cell nucleus, show the small-plaque effect. ionizing irradiation of herpes simplex vi ... | 1979 | 231946 |
| serological studies on the antigenic relationship between herpes simplex virus and varicella-zoster virus. | if crossreacting antibodies between varicella-zoster virus (vzv) and herpes simplex virus (hsv) exist, one would expect more positive reactions with vzv in a group of hsv positive patients than in a group of hsv negative patients. this statement can only apply to a group of individuals where positive and negative reactions with respect to hsv and vzv are evenly distributed. such a distribution can only be found among children. therefore, the relationship between hsv-1 and vzv was the only one wh ... | 1979 | 232073 |
| phosphorylation of a ribosomal protein and of virus-specific proteins in cells infected with herpes simplex virus. | in cells infected with herpes simplex virus a protein associated with the small subunit of ribosomes became phosphorylated. it was not detectably labelled with 14c-amino acids added after infection and is therefore probably a cellular protein. the phosphorylated ribosomal proteins from hsv-1- and hsv-2-infected cells were indistinguishable electrophoretically and had an apparent mol. wt. of about 48 000. phosphorylation of the 48k protein was detected 2 to 3 h after infection and reached a maxim ... | 1979 | 232130 |
| virus-specific dna sequences present in cells which carry the herpes simplex virus thymidine kinase gene. | two independently derived cell lines which carry the herpes simplex type 2 thymidine kinase gene have been examined for the presence of hsv-2-specific dna sequences. both cell lines contained 1 to 3 copies per cell of a sequence lying within map co-ordinates 0.2 to 0.4 of the hsv-2 genome. revertant cells, which contained no detectable thymidine kinase, did not contain this dna sequence. the failure of ecor1-restricted hsv-2 dna to act as a donor of the thymidine kinase gene in transformation ex ... | 1979 | 232133 |
| inhibition of herpes simplex virus-induced dna polymerase activity and viral dna replication by 9-(2-hydroxyethoxymethyl)guanine and its triphosphate. | the effect of the nucleoside analog 9-(2-hydroxyethoxymethyl)guanine (acycloguanosine) on herpes simplex virus type 1 dna synthesis was examined. acycloguanosine inhibited herpesvirus dna synthesis in virus-infected cells. the synthesis of host cell dna was only partially inhibited in actively growing cells at acycloguanosine concentrations several hundred-fold greater than the 50% effective dose for herpes simplex virus type 1. studies using partially purified enzymes revealed that the triphosp ... | 1979 | 232189 |
| the physical state of herpes simplex virus dna in infected human cells. | treatment of herpes simplex virus type 1 (hsv-1)-infected human embryo lung (hel) cells with phosphonoacetic acid (paa) resulted in complete inhibition of hsv dna replication. dna was extracted from paa-treated hel cells infected with hsv-1 and centrifuged in a neutral cscl density gradient. the hsv dna sequences in the nuclei of paa treated cells at 24 hr post infection banded at the same density as free hsv dna (1.725 g/cm3), but a significant amount of viral dna sequences were detected in the ... | 1979 | 232235 |
| isozyme studies on the association of the herpes simplex virus type 1 thymidine kinase gene with human chromosomes in somatic cell hybrids. | to investigate chromosomal site(s) of integration of the herpes simplex virus type 1 (hsv-1) thymidine kinase (tk) gene in biochemically transformed [hela(bu25)/kos 8-1] cells, these human cells which had been transformed by ultraviolet light-irradiated hsv-1 were fused with tk-negative mouse lm(tk-) cells, human-mouse somatic cell hybrid clones (lh81 clones 1-20) were isolated by hatg-ouabain selection and their chromosomes and isozymes were analyzed. electrophoretic and serological analyses sh ... | 1979 | 232492 |
| strain characteristics and features of ocular infection of herpes simplex virus type 1 isolates. | herpes simplex virus type 1 isolates from 63 patients with herpetic keratoconjunctivitis were investigated. for the purpose of detecting strain differences neurovirulence was determined in mice, and viral dna's were analyzed by digestion with ecori and hind iii restriction endonucleases. neurovirulence differed by a factor up to 10(5) between individual strains and proved to be independent of the ratio between infectious and noninfectious particles in the stocks used. the endonuclease cleavage p ... | 1979 | 232536 |
| chorioretinitis of the newborn with herpes simplex virus type 1. report of a case. | a disseminated herpes simplex virus (hsv) infection involving the central nervous system and accompanied by chrioretinitis in a a 3-week-old girl is described. the aetiologic diagnosis was established on the basis of vrius isolated from skin vesicles, and a significant rise in complement fixing antibodies to hsv type 1. the mode of transmission of the virus to the infant apparently was direct contact with an oral lesion in the mother, that was present at the time of delivery. the patient survive ... | 1979 | 232606 |
| resistance of c58 mice to primary systemic herpes simplex virus infection: macrophage dependence and t-cell independence. | the relative contribution of thymus-derived lymphocytes (t-cells) and of macrophages to resistance to primary infection with herpes simplex virus type 1 (hsv-1) was studied in c58 mice. resistance was dependent on macrophage competence, but was relatively independent of t-lymphocyte competence. although aging mice became progressively more deficient in functional t-cells, as demonstrated by a decreasing resistance to transplanted line ib leukemia and by declining responses to t-cell nitogens (co ... | 1979 | 232692 |
| experimental latent herpesvirus infection in rabbits, mice and hamsters: ultrastructure of the virus activation in explanted gasseric ganglia. | the frequency of latent infection as established in trigeminal ganglia of rabbits, mice and hamsters with human herpesvirus type 1 (hvh) was compared using two different virus strains. explantation proved to be effective in reisolation of hvh from ganglion tissue, which did not yield infectious virus at time of its removal. after healing of acute keratitis, the latent infection in homolateral gasseric ganglia of rabbits was detected at a relatively high frequency (60-80 per cent) up to 120 days ... | 1975 | 235193 |
| reproduction in unadapted and adapted cells at 40 degrees c of herpes simplex virus type 1 and type 2 strains. | differences in the reproduction of infectious virus in unadapted and adapted rabbit lung cells at 40 degrees c were observed among 8 strains of herpes simplex virus (hsv) type 1 and 6 strains of hsv type 2. the adapted cells represented a permissive system for some strains of both types at supraoptimal temperature. reproduction of hsv type 2 was not demonstrated in unadapted cells at 40 degrees c. strains of hsv type 1 reproduced to different titres in unadapted cells grown at 40 degrees c. | 1975 | 241225 |
| [immunological studies on herpes simplex virus (type 1 and 2) infection in guinea pig cornea (author's transl)]. | | 1979 | 316645 |
| acylovir in oral and ganglionic herpes simplex virus infections. | the local and trigeminal ganglionic therapeutic efficacy of two topical and systemic antiviral drugs was studied in mouse lips inoculated with herpes simplex virus type 1 after thermal injury. application of topical 3% acylovir (acycloguanosine) ointment three times daily for four days completely blocked the replication of virus in the lips, and the healing process was greatly accelerated compared with that in placebo-treated infected controls. however, neither the healing process nor the viral ... | 1979 | 316819 |
| fluorescent antibody study of diseased, end-stage human kidneys. | forty cases of diseased kidneys at end-stage were studied by fluorescent antibody technique in search for viral etiology of glomerulonephritis and other renal diseases. among these 40 cases, 12 (30%) were ascribed to immune complex disease because of detection of immunoglobulins and complement in glomeruli of the same kidney specimen. in 8 cases (20%) only complement was detected in glomeruli. in the remaining 50% neither complement nor immunoglobulin deposit was found in glomeruli. the etiologi ... | 1977 | 357100 |
| comparative evaluation of human leukocyte and fibroblast interferon in the prevention of herpes simplex virus keratitis in a monkey model. | the prophylactic potency of topically applied human leukocyte interferon and human fibroblast interferon was compared in a monkey model with herpes simplex virus type 1-induced keratitis. both interferons effectively prevented keratitis at 1.9 x 10(6) u of activity per ml but were ineffective at 1.9 x 10(3) u/ml. slight differences between human leukocyte interferon and human fibroblast interferon observed at the level of 1.9 x 10(5) u/ml were not statistically significant. | 1977 | 408274 |
| activities of 5,6-dihydro-5-azathymidine against herpes simplex virus infections in mice. | 5,6-dihydro-5-azathymidine (dhadt), a nucleoside antibiotic inhibitory for herpes simplex virus (hsv) in cell cultures (h. e. renis, antimicrob. agents chemother. 13: 613-617, 1978), was evaluated in mice with experimental hsv infections. dhadt protected mice infected with hsv type 1 (hsv-1) when the virus was inoculated intravenously and the drug was given by subcutaneous or oral routes. the activity observed was dependent on the dose and schedule of treatment. doses of 100 to 400 mg/kg given t ... | 1979 | 426515 |
| effect of pyran on latency after herpes simplex virus infections. | the immunomodulator pyran protected mice against both herpes simplex virus type 1 (hsv-1) and type 2 (hsv-2) infections. in infections of the lip with hsv-1, prophylactic administration of pyran reduced the severity of the herpetic lesions and enhanced their resolution, but did not decrease the high incidence of development of latent hsv-1 infection of the trigeminal ganglia. in vaginal infections with hsv-2, prophylactic administration of pyran either systemically or locally reduced mortality, ... | 1979 | 464586 |
| characteristics of herpesvirus mutants resistant to phosphonoformate and phosphonoacetate. | mutants of herpes simplex virus type 1, resistant to phosphonoformate (pfa) and phosphonoacetate (paa), have been selected in cell culture. the pfa-resistant mutant (hsv-pfa(r)) and the paa-resistant mutant (hsv-paa(r)) were also resistant to paa and pfa, respectively. this cross-resistance indicates that pfa and paa interact at the same site. the hsv-pfa(r) had a decreased susceptibility to vidarabine, but no difference in sensitivity to idoxuridine (5-iodo-2'-deoxyuridine) was observed when co ... | 1979 | 475360 |
| recurrent herpetic angular blepharitis in an adult. | a 36-year-old man had several episodes of left angular blepharoconjunctivitis associated with upper respiratory infections, herpes labialis, or emotional stress. the clinical diagnosis had always been a blepharitis of bacterial origin. during the last attack, cytologic examination of a scraping obtained from an eyelid pustule vesicle revealed multinucleated keratinocytes, diagnostic of a herpetic infection. virologic studies proved that herpes simplex virus type 1 was the causal agent. | 1979 | 507148 |
| latent infections of sensory ganglia as influenced by phosphonoformate treatment of herpes simplex virus-induced skin infections in hairless mice. | topical treatment with 3% phosphonoformate of herpes simplex virus type 1 (hsv)-induced skin infections of hairless mice reduced the severity of skin lesions when the treatment was initiated 3 h after virus inoculation in the lumbosacral area or 3 and 24 h after inoculation in the orofacial area. the mortality was significantly reduced in lumbosacral-infected mice and was completely prevented in orofacial-infected mice when the treatment was initiated with a delay of 24 h after virus inoculation ... | 1979 | 507784 |
| relative potencies of different anti-herpes agents in the topical treatment of cutaneous herpes simplex virus infection of athymic nude mice. | thirteen established anti-herpes compounds have been directly compared in a single assay system for their effects on the development of herpetic skin lesions, and mortality associated therewith, in athymic nude (nu/nu) mice inoculated intracutaneously with herpes simplex virus type 1 (kos). when applied topically (at 1% in a water-soluble ointment), phosphonoacetic acid, e-5-(2-bromovinyl)-2'-deoxyuridine, acycloguanosine, and trisodium phosphonoformate emerged as the most active agents. | 1979 | 526011 |
| rabbit beta-globin mrna production in mouse l cells transformed with cloned rabbit beta-globin chromosomal dna. | mouse thymidine kinase-negative l cells were transformed with a cloned rabbit chromosomal beta-globin gene linked to the clone thymidine kinase gene of herpes simplex virus type 1. most thymidine kinase-positive cell lines contained one or more copies of rabbit beta-globin dna and produced up to 2,000 copies of rabbit beta-globin rna per cell indistinguishable from its authentic counterpart. no mouse beta-globin mrna was detected. | 1979 | 551266 |
| herpes simplex infection and experimental allergic encephalomyelitis. an experimental model system for reactivation of eae. | the effects of herpes simplex virus type 1 (hsv-1) infection on the course of experimental allergic encephalomyelitis (eae) were studied in rats. fifty percent of animals given two intracerebral injections of hsv-1, one before and one after induction of eae, showed clinical and pathologic evidence of recently exacerbated eae 16 days after the second hsv-1 injection. when hsv-1 injections were administered subcutaneously before and after induction of eae, 45% of survivors showed pathologic change ... | 1977 | 559269 |
| [experimental protection with tyrothrizin against herpes simplex virus infections in mice]. | using 12-day old baby mice, strain nmri, the ld50 of a herpes simplex virus type 1 was calculated. intragastric inoculation of the mice was carried out applying a stomach tube. lethality of the mice was reduced from 67% to 23% by pre-incubation of the virus suspension for 30 min at 37 degrees c in the presence of 0.2 mg tyrothricin/ml. the effect was statistically significant and could be produced only after direct contact between tyrothricin and virus. under the same conditions there was no eff ... | 1979 | 582119 |
| a possible role for polymorphonuclear leucocytes in the defence against recrudescent herpes simplex virus infection in man. | we have used a 51cr release assay to demonstrate that human polymorphonuclear leucocytes (pmnl) can damage herpes simplex infected target cells sensitized with antiviral antibody. effective sensitizing antibodies were found in both serum and saliva of all those persons tested who were subject to recurrent cold sores. pmnl were much less effective as killer cells than peripheral blood mononuclear cells, but as they are the predominant inflammatory cell with the hsv1 lesion they may be, quantitati ... | 1978 | 640713 |
| herpetic whitlow: a manifestation of primary infection with herpes simplex virus type 1 or type 2. | | 1978 | 659928 |
| antiviral activity of extracts from marine algae. | extracts of two species of marine algae, constantinea simplex and farlowia mollis, were tested for antiviral activity in tissue culture and in experimental infections of mice. treatment of confluent mouse embryo fibroblast cell monolayers with either compound before viral inoculation was effective in inhibiting the replication of herpes simplex virus type 1 and type 2, vaccinia virus, and vesicular stomatitis virus, but not encephalomyocarditis virus, semliki forest virus, or murine cytomegalovi ... | 1978 | 686707 |
| cellular and humoral immune responses to herpes simplex virus during and after primary gingivostomatitis. | a total of 17 children, aged 1 to 15 years, with gingivostomatitis were investigated to follow the development of immune parameters in those who suffered from herpes simplex virus stomatitis. mouth swabs were obtained during the acute attack. blood samples were collected on this occasion and again about 3 weeks later. humoral immunity to herpes simplex virus was investigated by a complement fixation test and by an antibody-dependent cell-mediated cytotoxicity test. cell-mediated immunity was inv ... | 1978 | 730366 |
| role of interferon in the pathogenesis of herpes simplex virus disease in mice. | the use of a potent antiinterferon serum has demonstrated the importance of the interferon response in host resistance to infection with herpes simplex virus (hsv). inoculation of mice with potent antiinterferon globulin was associated with a very marked effect on the evolution of hsv disease, as shown by an acclerated appearance of signs of disease and death. in a total of seven experiments, i.v. administration of a sheep antimouse interferon globulin (titre 4 x 10(5)) resulted in the early app ... | 1978 | 751900 |
| effects of adenine arabinoside on cellular immune mechanisms in humans. | in vitro lymphocyte blastogenic responses to the commonly employed mitogens, phytohemagglutinin, pokeweed, and concanavalin a, were evaluated when adenine arabinoside (ara-a) in a concentration of 3 mug/ml was added to the culture materials. similarly, blastogenic and cytotoxic responses to cell cultures persistently infected with herpes simplex virus 1, herpes simplex virus 2, and varicella-zoster virus were determined in the presence of ara-a. no depression of these cellular immune responses b ... | 1975 | 1137371 |
| comparison of the antiviral effects of 5-methoxymethyl-deoxyuridine with 5-iododeoxyuridine, cytosine arabinoside, and adenine arabinoside. | the antiviral activity of 5-methoxymethyl-2'-deoxyuridine (mmudr) was compared with that of 5-iodo-2'-deoxyuridine (iudr), cytosine arabinoside (ara-c), and adenine arabinoside (ara-a). at concentrations of 2 to 4 mug/ml, mmudr was inhibitory to herpes simplex virus type 1, but concentrations as high as 128 mug/ml were not inhibitory to three other herpesviruses tested (equine rhinopneumonitis virus, murine cytomegalovirus, and feline rhinopneumonitis virus) or to vaccinia virus. the other nucle ... | 1975 | 1239978 |
| herpes simplex encephalitis simulating systemic embolism. | a case of herpes simplex virus encephalitis is described in a patient known to suffer from chronic atrial fibrillation and whose clinical symptoms suggested cerebral embolism of cardiac origin. the final diagnosis was based on the presence of herpes simplex virus type 1 antibodies in the cerebrospinal fluid and a significant rise in antibody titers in the serum. the patient regained consciousness after craniotomy and treatment with idoxuridine. the case emphasizes that the possibility of herpeti ... | 1976 | 1262205 |
| assembly of conformation-dependent neutralizing domains on glycoprotein b of human cytomegalovirus. | we analysed the antigenic properties of human cytomegalovirus (cmv) glycoprotein b (gb) by constructing a set of deletion derivatives lacking different portions of the carboxy terminus and reacting them with a panel of monoclonal antibodies with neutralizing activity. we found that two novel antigenic domains that bind neutralizing antibodies were assembled on truncated forms of gb, one in the amino-terminal half and one that spans the midregion of the molecule. assembly of the conformation-depe ... | 1992 | 1279102 |
| cyclic amp-mediated inhibition of vesicular stomatitis virus and herpes simplex virus replication in mouse macrophage-like cells. | in this study, we have analysed the effects of camp inducers on the multiplication of vesicular stomatitis virus (vsv) and herpes simplex virus type 1 (hsv-1) in mouse macrophage-like cells. the addition of dibutyryl camp (db-camp) or cholera toxin to resting peritoneal macrophages aged in vitro or p388d1 cells resulted in a 10- to 100-fold reduction of vsv yield compared to control cultures. in contrast, no camp-dependent inhibition was found in vsv-infected l929 cells. in macrophage-like cells ... | 1992 | 1279103 |
| distinct monoclonal antibodies separately label the hexons or the pentons of herpes simplex virus capsid. | the surface shell of the capsid of herpes simplex virus type 1 (hsv-1) is 15 nm thick and 125 nm in outer diameter and has the form of an icosahedral (t = 16) surface lattice, composed of 150 hexons and 12 pentons. hexons are traversed by axial channels and have six-fold symmetric external protrusions, separated by triangular nodules ("triplexes"). pentons resemble hexons morphologically, apart from their different order of symmetry. to localize vp5, the major capsid protein, in the shell struct ... | 1992 | 1280828 |
| [the isolation and characteristics of monoclonal antibodies to different proteins of the herpes simplex virus types 1 and 2]. | the authors prepared 156 mouse hybridomas producing monoclonal (mca) antibodies to type- and group-specific antigenic determinants of hsv-1 and hsv-2. seven of them were studied at length by western blot and radioimmunoprecipitation methods. the cell lines 1h97 and 2h141 were shown to produce immunoglobulins of g2 beta and m class, respectively, and were directed against group-specific antigenic determinants of the major nucleocapsid protein p150. the cell lines 1h38 and 1h110 produced immunoglo ... | 1992 | 1281945 |
| sequence determination and genetic content of an 8.9-kb restriction fragment in the short unique region and the internal inverted repeat of marek's disease virus type 1 dna. | the dna sequence (8.9 kb) covering about 70% of the short unique region (us) and part of the short inverted repeat of the marek's disease virus type 1 ga strain was determined. computer analysis of the sequence showed the presence of nine potential open reading frames (orfs), consisting of more than 300 nucleotides in the us region. of these orfs, four were found to be homologous to us10 (minor virion protein), us3 (protein kinase), us2, and us6 (gd) in the us region of alpha-herpesvirus herpes ... | 1992 | 1282282 |
| [the inhibitory effects of catechin derivatives on the activities of human immunodeficiency virus reverse transcriptase and dna polymerases]. | catechin derivatives including (-)-epicatechin gallate (ecg), (-)-epigallocatechin gallate (egcg), (-)-epigallocatechin (egc) and green tea extract (gte) were found to inhibit the activities of cloned human immunodeficiency virus type 1 reverse transcriptase (hiv-1 rt), duck hepatitis b virus replication complexes reverse transcriptase (dhbv rcs rt), herpes simplex virus 1 dna polymerase (hsv-1 dnap) and cow thymus dna polymerase alpha (ct dnap alpha). egcg and ecg were shown to be very potent i ... | 1992 | 1284389 |
| detection of herpesvirus dna in the large intestine of patients with ulcerative colitis and crohn's disease using the nested polymerase chain reaction. | the prevalence of herpesvirus dna was examined in inflammatory bowel disease tissue. dna was extracted from resection and biopsy specimens of the large intestine from patients with ulcerative colitis (n = 21), patients with crohn's disease (n = 29), and patients with noninflammatory bowel disease (controls) (n = 21). the nested polymerase chain reaction was used to detect viral dna using primer pairs specific for either cytomegalovirus (cmv), herpes simplex virus 1 (hsv1), human herpesvirus 6 (h ... | 1992 | 1287131 |
| [herpes simplex virus infection in pregnancy: epidemiologic, diagnostic and therapeutic data. an unusual case of hsv-1 in monozygotic twins]. | the authors report a case of two pre-term monocorial twins affected by hsv-1 infection (herpes simplex virus type 1), with generalized sepsis and involvement of the central nervous system (cns), born by mother with primary infection who presented a typical vesicular eruption a week before delivery. as the hsv-1 was a disseminated type and the cns was involved in both the twins, the diagnosis was based on clinical and laboratory findings (specific igm and igg) and on the use of delpech-lichtblau ... | 1992 | 1287430 |
| susceptibility of +/+, +/nu and nu/nu balb/c mice to ocular herpes simplex virus infection. | athymic (nude) mice have played an important role in defining the function of the immune system and its role in infectious diseases. in the majority of these studies, heterozygous +/nu mice have been used as normal controls for the nu/nu mice, and it has been assumed that +/nu mice have essentially normal immune systems. we have compared the response of +/+, +/nu and nu/nu balb/c mice following ocular infection with hsv-1 and have found that +/nu mice develop significantly more severe blephariti ... | 1992 | 1287512 |
| neonatal herpes simplex: royal women's hospital 10-year experience with management guidelines for herpes in pregnancy. | in the 10-year period 1982-1991 inclusive, 6 cases of neonatal herpes simplex were diagnosed at the royal women's hospital, melbourne (1 disseminated, 1 pneumonitis, 4 cutaneous [1 with central nervous system involvement]), resulting in an incidence of 1 in 11,000 livebirths for this population. three cases were due to hsv 1 and 3 to hsv 2. in no case did the mother have a recent or past history of overt genital herpes infection. two cases were acquired in utero, an uncommonly recognized form of ... | 1992 | 1290430 |
| oral herpes simplex virus type 1 infection following cadaveric renal transplantation in a young type 1 diabetic female. the role of acyclovir: a case report. | oral infection with herpes simplex virus (hsv) is a frequent and well documented complication in immunosuppressed individuals including patients on immunosuppressive medication. we report the development of severe oral infection with hsv type 1 in a 34 year old woman with type 1 diabetes mellitus and end stage renal disease (esrd) following cadaveric renal transplantation at the western general hospital, edinburgh. the role of acyclovir in therapy and chemoprophylaxis is discussed. | 1992 | 1298640 |
| in situ hybridisation: principles and applications. | in situ hybridisation (ish) is based on the complementary pairing of labelled dna or rna probes with normal or abnormal nucleic acid sequences in intact chromosomes, cells or tissue sections. compared with other molecular biology techniques applicable to anatomical pathology, ish enjoys better rapport with histopathologists because of its similarity to immunohistochemistry. it has the unique advantage over other molecular biology techniques--largely based on probe hybridisation with nucleic acid ... | 1992 | 1304627 |
| [influence of high dose intravenous immunoglobulins (ivig) on hsv-1 infection in immunosuppressed mice]. | influence of low and high doses of intravenous immunoglobulin (ivig) was compared on the basis of survival studies of mice infected with temperature sensitive (ts 28 degrees c) clone of herpes simplex virus (hsv-1) after previous treatment with cyclophosphamide (cy), sandoglobulin containing intact igg and gamma-venin containing only fragments of (fab)2 igg were used. it was found that 4 and 16 mg doses protected animals in contrast to the 32 mg dose of sandoglobulin which protected mice in only ... | 1992 | 1305921 |
| high levels of enhanced reactivation of herpes simplex virus in skin fibroblasts from various hereditary cancer-prone syndromes. | the dose response of the enhanced reactivation (er) of herpes simplex virus type 1 has been studied in uv-irradiated normal human skin fibroblasts and fibroblasts from the following hereditary cancer-prone syndromes: retinoblastoma, aniridia, polyposis coli, neurofibromatosis type 1 and 2, dysplastic nevus syndrome, von hippel-lindau syndrome, multiple endocrine neoplasia type 2, and bloom's syndrome. surprisingly, much higher levels of er were observed in all these genetically heterogeneous her ... | 1992 | 1309228 |
| deletion of the vp16 open reading frame of herpes simplex virus type 1. | vp16 (also called vmw65 and alpha tif) is a structural protein of herpes simplex virus type 1 (hsv-1) that trans-induces hsv-1 immediate-early gene transcription. this report describes an hsv-1 vp16 deletion mutant that was constructed and propagated in a cell line transformed with a vp16 expression vector. the vp16 deletion mutant replicated like wild-type hsv-1 during infection of the vp16-expressing cell line. deletion mutant virions propagated in this cell line contained wild-type, cell-deri ... | 1992 | 1309245 |
| herpes simplex virus type 1 recombination: role of dna replication and viral a sequences. | during the course of infection, elements of the herpes simplex virus type 1 (hsv-1) genome undergo inversion, a process that is believed to occur through the viral a sequences. to investigate the mechanism of this recombinational event, we have developed an assay that detects the deletion of dna segments flanked by directly repeated a sequences in plasmids transiently maintained in vero cells. with this assay, we have observed a high frequency of recombination (approximately 8%) in plasmids that ... | 1992 | 1309247 |
| construction and properties of a mutant of herpes simplex virus type 1 with glycoprotein h coding sequences deleted. | a mutant of herpes simplex virus type 1 (hsv-1) in which glycoprotein h (gh) coding sequences were deleted and replaced by the escherichia coli lacz gene under the control of the human cytomegalovirus ie-1 gene promoter was constructed. the mutant was propagated in vero cells which contained multiple copies of the hsv-1 gh gene under the control of the hsv-1 gd promoter and which therefore provide gh in trans following hsv-1 infection. phenotypically gh-negative virions were obtained by a single ... | 1992 | 1309250 |
| the varicella-zoster virus immediate-early protein ie62 is a major component of virus particles. | varicella-zoster virus (vzv) open reading frame (orf) 62 potentially encodes a protein with considerable amino acid homology to the herpes simplex virus (hsv) immediate-early regulatory polypeptide icp4 (or ie3). to identify and characterize its protein product(s) (ie62), we used a rabbit antiserum prepared against a synthetic peptide corresponding to the c-terminal 13 amino acids of the predicted protein. this antiserum reacted with phosphorylated polypeptides of 175 to 180 kda that were made i ... | 1992 | 1309252 |
| the fibroblast growth factor receptor is not required for herpes simplex virus type 1 infection. | the early events mediating herpes simplex virus type 1 (hsv-1) infection include virion attachment to cell surface heparan sulfates and subsequent penetration. recent evidence has suggested that the high-affinity fibroblast growth factor (fgf) receptor mediates hsv-1 entry. this report presents three lines of experimental evidence showing that the high-affinity fgf receptor is not required for hsv-1 infection. first, rat l6 myoblasts lacking fgf receptors were as susceptible to hsv-1 infection a ... | 1992 | 1309254 |
| the ul5 gene of herpes simplex virus type 1: isolation of a lacz insertion mutant and association of the ul5 gene product with other members of the helicase-primase complex. | the ul5 gene product is required continuously during viral dna synthesis (l. zhu and s. k. weller, virology 166:366-378, 1988) and has been shown to be a component of a three protein helicase-primase complex encoded by herpes simplex virus type 1 (j. j. crute, t. tsurumi, l. zhu, s. k. weller, p.d. olivo, m. d. challberg, e. s. mocarski, and i. r. lehman, proc. natl. acad. sci. usa 86:2186-2189, 1989). the other members of the complex are viral proteins encoded by genes ul8 and ul52. in this stu ... | 1992 | 1309255 |
| the six conserved helicase motifs of the ul5 gene product, a component of the herpes simplex virus type 1 helicase-primase, are essential for its function. | the ul5 protein of herpes simplex virus type 1, one component of the viral helicase-primase complex, contains six sequence motifs found in all members of a superfamily of dna and rna helicases. although this superfamily contains more than 20 members ranging from bacteria to mammalian cells and their viruses, the importance of these motifs has not been addressed experimentally for any one of them. in this study, we have examined the functional significance of these six motifs for the ul5 protein ... | 1992 | 1309257 |
| the unique sequence of the herpes simplex virus 1 l component contains an additional translated open reading frame designated ul49.5. | we present evidence for the existence of an additional herpes simplex virus 1 gene designated ul49.5. the sequence, located between genes ul49 and ul50, predicts a hydrophobic protein with 91 amino acids. attempts to delete ul49.5 were not successful. to demonstrate that ul49.5 is expressed, we made two recombinant viruses. first, we inserted in frame an oligonucleotide encoding a 15-amino-acid epitope known to react with a monoclonal antibody. this gene, consisting of the authentic promoter and ... | 1992 | 1309263 |
| evidence that the herpes simplex virus immediate early protein icp27 acts post-transcriptionally during infection to regulate gene expression. | the herpes simplex virus (hsv-1) immediate early protein icp27 is a regulatory protein which is essential for virus replication. the phenotype of temperature-sensitive and deletion mutants in icp27 includes overexpression of some immediate early and early gene products and greatly reduced levels of late gene products. to determine whether regulation by icp27 occurs primarily at the transcriptional level, we have studied the expression of two immediate early products (icp4 and icp27) and two late ... | 1992 | 1309283 |
| processing of the herpes simplex virus assembly protein icp35 near its carboxy terminal end requires the product of the whole of the ul26 reading frame. | the herpes simplex virus (hsv) type 1 assembly protein icp35 consists of a family of polypeptides, ranging in molecular weight from about 45,000-39,000. the lower molecular weight forms of icp35 are derived from the higher molecular weight species by slow post-translational modification. the reading frame of gene ul26 and the region within this gene which exhibited homology to the cytomegalovirus assembly protein, the analogous protein to icp35, were expressed separately under immediate-early (i ... | 1992 | 1309284 |
| detection of viral dna within skin of healed recurrent herpes simplex infection and erythema multiforme lesions. | the polymerase chain reaction (pcr) was used to detect hsv dna in genomic dna extracted from skin biopsies obtained from healed skin of five patients with hyperpigmented macules following recurrent cutaneous hsv infections and from eight patients with hsv-associated erythema multiforme (em). a 92-bp hsv-1 dna fragment was found in all the skin biopsies from the site of recurrent hsv infection and in five of eight (62%) biopsies from the em patients. virus dna was not found in tissues distant fro ... | 1992 | 1309462 |
| cell fusion caused by herpes simplex virus-1 (hsv-1) strains tsb5 and mp is inhibited at ph 6.7 and ph 7.0. | we investigated the effect of different ph conditions on vero cell cultures infected with herpes simplex virus-1 (hsv-1) wild-type strain kos, and syncytial mutants hsv-1 hfem (tsb5) and hsv-1 mp (mp). cell fusion was inhibited when infected cells were continuously incubated with culture media adjusted to ph 6.7 or ph 7.0. inhibition of cell fusion was rapidly reversible when infected cell cultures were returned to ph 7.5. the rate of synthesis and cell-surface expression of virus-specified glyc ... | 1992 | 1309636 |
| immunohistopathologic findings in herpes simplex virus chorioretinitis in the von szily model. | after anterior chamber inoculation of herpes simplex virus type 1 (hsv-1), some mice have a characteristic pattern of ocular disease, including ipsilateral anterior uveitis, relative sparing of the ipsilateral retina, and necrotizing contralateral chorioretinitis. it was reported previously that susceptibility to the contralateral chorioretinitis is associated with the igh-1 locus; congenic mice differing at this locus have different rates of contralateral disease. the immunohistopathologic find ... | 1992 | 1309731 |
| purification and characterization of the carboxyl-terminal transactivation domain of vmw65 from herpes simplex virus type 1. | a glutathione s-transferase fusion to the cooh-terminal acidic transactivation domain of vmw65 from herpes simplex virus type 1 was overexpressed in escherichia coli and isolated by affinity chromatography on glutathione-sepharose. following cleavage of the fusion protein with thrombin, the transactivation domain was purified to homogeneity by ion exchange chromatography yielding approximately 0.6 mg of protein/liter of bacterial culture. equilibrium sedimentation analysis showed the purified po ... | 1992 | 1309782 |
| herpes simplex virus infection as a risk factor for human immunodeficiency virus infection in heterosexuals. | to determine if infection with herpes simplex virus (hsv) type 2 is associated with human immunodeficiency virus (hiv) type 1 infection among patients attending sexually transmitted diseases clinics, a case-control study was done on coded sera from 179 hiv-1-infected patients and 367 age-, race-, and gender-matched hiv-1-seronegative patients. although only 13 (2.3%) of 546 patients had a history of genital herpes treatment, 72% and 56.6%, respectively, had serologic evidence of prior infection ... | 1992 | 1309846 |
| the unique n terminus of the herpes simplex virus type 1 large subunit is not required for ribonucleotide reductase activity. | using purified bacterially expressed herpes simplex virus type 1 ribonucleotide reductase large subunit (r1) and the proteolytic enzymes chymotrypsin and trypsin, we have generated stable n-terminal truncations. chymotrypsin removes 246 amino acids from the amino terminus to produce a fragment (dn246r1) which retains full enzymic activity and affinity for the small subunit (r2). treatment of r1 with trypsin produces a 120k protein and a cleavage at amino acid residue 305 to produce a fragment (d ... | 1992 | 1309856 |
| nucleotide sequence analysis of a homologue of herpes simplex virus type 1 gene us9 found in the genome of simian herpes b virus. | the 10k gene of simian herpes b virus (shbv) has been located and the nucleotide sequence determined. its relationship to homologous genes in other herpes-viruses has been examined. the shbv 10k gene exhibits a closer relationship to its homologue in hsv-1 than to those in the other viruses studied. nucleotide sequence identity of 61% was found between the hsv-1 and shbv 10k genes, and 57% identity was found between the corresponding predicted protein sequences. a comparison of the amino acid se ... | 1992 | 1309859 |
| the icp4 binding sites in the herpes simplex virus type 1 glycoprotein d (gd) promoter are not essential for efficient gd transcription during virus infection. | activation of the early and late genes of herpes simplex virus type 1 during infection in tissue culture requires functional immediate-early regulatory protein icp4. icp4 is a specific dna-binding protein which recognizes a variety of dna sequences, many of which contain the consensus atcgtc. in general, mutations which impair the ability of icp4 to bind to dna also eliminate its ability to activate viral early and late promoters both in transfection assays and in the infected cell. however, the ... | 1992 | 1309905 |
| herpes simplex virus infection can occur without involvement of the fibroblast growth factor receptor. | basic fibroblast growth factor (bfgf) has been reported to block uptake of herpes simplex virus type 1 (hsv-1) and plaque formation on arterial smooth muscle cells, suggesting a role for the bfgf receptor in hsv entry (r. j. kaner, a. baird, a. mansukhani, c. basilico, b. d. summers, r. z. florkiewicz, and d. p. hajjar, science 248:1410-1413, 1990). we confirmed the effect of bfgf on infection of this cell type with hsv-1 and hsv-2 and found the same result with umbilical vein endothelial cells. ... | 1992 | 1309916 |
| herpes simplex virus type 1 replicates in the lens and induces cataracts in mice. | in a mouse model, cataracts were produced after corneal inoculation with herpes simplex virus type 1 (hsv-1) strain f. viral antigen was detected in vivo by the immunoperoxidase technique in lens fibers and occasionally in lens epithelial cell nuclei at 4 and 5 days postinoculation. hsv-1 replication within the lens was further demonstrated by virus isolation from lenses that had been inoculated in vitro. lens lesions in mice were present as early as 4 days postinoculation and by later times (23 ... | 1992 | 1309928 |
| early induction of dna single-stranded breaks in cells infected by herpes simplex virus type 1. | in cells infected with herpesviruses a series of host-cell nuclear changes can be observed in a temporal sequence, such changes include chromosome aberrations. the precise mechanism by which virus infection produces chromosome damage is not known. previous studies have revealed modifications in the properties of chromatin from infected cells, but such modifications are not due to extensive breakdown of host dna or alteration of the nucleosomal structure in bulk host chromatin. we have adapted an ... | 1992 | 1309995 |
| structure-function studies of peptides inhibiting the ribonucleotide reductase activity of herpes simplex virus type i. | ac-tyr298-ala299-gly300-thr301-val302-i le303-asn304-asp305-leu306-oh (ac-vzv r2-(298-306)) represents the acetylated form of the c-terminus of varicella-zoster virus (vzv) ribonucleotide reductase subunit 2 (r2). this peptide possesses a high degree of homology with the c-terminus nonapeptide of the herpes simplex virus (hsv) type i and ii ribonucleotide reductase r2 protein and is 15 times more potent than the latter in its in vitro inhibition of hsv-1 reductase activity. accordingly, a new se ... | 1992 | 1310120 |
| intracellular metabolism of the antiherpes agent (s)-1-[3-hydroxy-2-(phosphonylmethoxy)propyl]cytosine. | (s)-1-[3-hydroxy-2-(phosphonylmethoxy)propyl]cytosine (hpmpc) is an antiviral phosphonate nucleotide analogue that displays activity against a range of herpesviruses. anion exchange high performance liquid chromatography analysis of the 60% methanol extract from [14c]hpmpc-treated cells reveals the formation of three major metabolites. two of these were identified as phosphorylated forms of hpmpc, hpmpc phosphate, and hpmpc diphosphate, by liberation of hpmpc upon acid digestion and coelution wi ... | 1992 | 1310143 |
| in vitro characterization of the hsv-1 ul53 gene product. | the ul53 gene is the locus altered in many syncytial mutants of herpes simplex virus type 1 (hsv-1). however, the protein encoded by this gene has not been characterized. in this study, the ul53 protein was produced by in vitro translation of in vitro-transcribed ul53 rna. post-translational processing of the protein was studied by translation in the presence of pancreatic microsomal membranes. these microsomes carry out the processing steps that normally occur in the rough endoplasmic reticulum ... | 1992 | 1310186 |
| transactivation of the hiv-1 ltr by hsv-1 immediate-early genes. | herpes simplex virus type 1 (hsv-1) activates transcription from the long terminal repeat (ltr) promoter region of the human immunodeficiency virus type 1 (hiv-1). hsv-1 immediate-early (ie) genes icp0 and icp4 are thought to be important mediators of this process, which is known to involve the induction of the cellular activators nf-kappa b and sp1. we demonstrate that icp0 and icp4 transactivation of the ltr is largely dependent on the presence of nf-kappa b and sp1 binding sites. however, in ... | 1992 | 1310199 |
| triple-helix formation is compatible with an adjacent dna-protein complex. | the effect of oligonucleotide-directed triple-helix formation on the binding of a protein to an immediately adjacent sequence has been examined. a double-stranded oligonucleotide was designed with a target site for the binding of a pyrimidine oligonucleotide located immediately adjacent to the recognition sequence for the herpes simplex virus type 1 (hsv-1) origin of replication binding protein, which is encoded by the ul9 gene of hsv-1. since the optimal conditions for the binding of the ul9 pr ... | 1992 | 1310426 |
| replication, latent infection, and reactivation in neuronal culture with a herpes simplex virus thymidine kinase-negative mutant. | herpes simplex virus type 1 (hsv-1) mutant viruses lacking functional viral thymidine kinase activity are reported to be incapable of replication in neurons. to investigate the role of viral thymidine kinase (tk) activity in the hsv-1 infection of the neuron, we studied a thymidine kinase-negative (tk-) mutant virus engineered to eliminate tk function without affecting the other known transcripts encoded in this region of the genome. studies using the mouse eye model demonstrated that the mutant ... | 1992 | 1310559 |
| synthesis, antiproliferative, and antiviral activity of certain 4-aminopyrrolo[2,3-d]pyridazine nucleosides: an entry into a novel series of adenosine analogues. | the preparation of novel heterocyclic base modified adenosine analogues, the 4-aminopyrrolo[2,3-d]pyridazine nucleosides, is described. crucial to their successful preparation was the use of the pyrrole glycoside intermediates 3-cyano-2-formyl-1-(2,3,5-tri-o-benzyl-beta-d-ribofuranosyl)pyrrole (11) and 3-cyano-2-formyl-1-(2,3,5-tri-o-benzyl-beta-d-arabinofuranosyl)pyrrole (17). treatment of 11 and 17 with hydrazine dihydrochloride followed by treatment with boron trichloride provided 4-amino-1-b ... | 1992 | 1310744 |
| human cd8+ herpes simplex virus-specific cytotoxic t-lymphocyte clones recognize diverse virion protein antigens. | the role of the hla class i-restricted, cd8+, herpes simplex virus (hsv)-specific cytotoxic t lymphocytes (ctl) in the control of human hsv infections is controversial because previous reports suggest that a substantial portion of the antigen-specific lytic response is mediated by cd4+ cells. to address this question directly, we isolated hsv-specific cd8+ ctl clones from a patient with recurrent genital herpes. these ctl were cloned by coculturing responder peripheral blood mononuclear cells (p ... | 1992 | 1310769 |
| polymerase chain reaction amplification of herpes simplex virus dna from clinical samples. | the polymerase chain reaction was used to amplify herpes simplex virus (hsv) dna from 79 clinical specimens from the female genital tract, and the results were compared with cell culture. combining the polymerase chain reaction with visualization of amplified products using a direct gel analysis, hsv dna was detected in 38 specimens, six of which were negative for virus by cell culture. hybridization of the amplified products detected hsv in three other specimens. one specimen was positive for h ... | 1992 | 1310807 |