| effect of 5-bromo-2'-deoxyuridine on the in vitro dna synthesizing activity of polyoma virus-infected cells. | the in vitro synthesizing activity of uninfected and polyoma virus-infected mouse embryo (me) cells was investigated by measuring the incorporation of 3h-thymidine (dtr) into acidinsoluble material in cell-free extracts. the character of the enzyme reaction was described in uninfected cells and an activating effect of 0.01 m dithiothreitol (dtt) was observed. a variation of the enzyme activity in the course of cultivation of me cells in primary culture was demonstrated. the induction of the dna ... | 1978 | 25003 |
| dengue viral antigens in host cell membranes. | membranes from type 2 dengue virus (den-2) infected bhk cells, when used as immunogen, elicit antibodies detectable by complement fixation, hemagglutination inhibition and plaque reduction neutralization tests with suckling-mouse-brain-derived den-antigens. membrane fractions from infected cells separated by sucrose density gradient centrifugation were employed as antigen in serological tests with anti-den-2 infected suckling mouse brain hyperimmune mouse serum and hyperimmune mouse ascitic flui ... | 1978 | 25005 |
| identification and properties of the nicotinamide adenine dinucleotide (phosphate)+-dependent malic enzyme in mouse ascites tumor mitochondria. | | 1978 | 25711 |
| toxoplasma antibodies and spontaneous abortion. | one hundred and fifty-two women with spontaneous abortion were investigated by hemagglutination (ha) and immunofluorescence antibody (ifa) tests for toxoplasmosis. in 48 cases, quantitive immunoglobulin (ig) studies and mouse inoculation with gestational material were performed. positive toxoplasma antibody titers were observed in 62 cases (40.8%) using ha and in 52 cases (38.2%) using ifa. this prevalence was significantly higher than that observed in 80 normal women who served as controls. tox ... | 1978 | 25801 |
| the development of lysosomal apparatus. i. lysosomal enzyme activities in the liver of mice at perinatal stages and those of their mothers. | the enzymatic activity of five acid hydrolases: acid phosphatase, arylsulfatase a, deoxyribonuclease, beta-glucuronidase, and cathepsin d, was assayed in fetal (fifteenth and eighteenth days of pregnancy) and neonatal (days 0, 5, 10, and 15 post-partum) mouse liver. with the exception of cathepsin d, the activity increased around birth to levels varying according to the enzyme. histochemical observations of other authors appear to justify, at least in part, the present results, which indicate th ... | 1978 | 25903 |
| effect of lithium on the membrane-bound magnesium-dependent atpase of mouse neuroblastoma cells. | | 1978 | 26497 |
| tricyclic antidepressants block histamine h1 receptors of mouse neuroblastoma cells. | | 1978 | 26884 |
| the effect of cyclophosphamide and methotrexate on the 'field effect' or unresponsiveness observed in the rat and mouse gvhr. | the field effect is the decreased biological response to a second challenge in the graft versus host reaction (gvhr). treatment of recipeint rats or mice during the initial gvhr by methotrexate or cyclophosphamide effected the secondary unresponsivienss. methotrexate and cyclophosphamide demonstrated different temporal responses indicating different mechanisms of their immunosuppresdive activities. | 1975 | 27086 |
| changes in the distribution of gamma-glutamyl transferase in the organs of the mouse as a function of development. | in the mouse, gamma-glutamyl transferase distribution changes with development in the kidney and the liver; on the contrary, its activity remains almost equal and low in other organs. in the liver, a low activity is observed in the fetus and the new-born up to the 3rd day of life; then it is no more measurable. in the kidney, the low enzyme activity of the fetus is multiplied by 10 in the 10 first days of life and by 50 in the 6 first weeks. | 1978 | 27317 |
| effects of acidified fetal bovine serum on the fibrinolytic activity and growth of cells in culture. | the fibrinolytic activity of cells in culture varied with the type of serum employed in the growth medium. degradation of iodinated fibrin occurred slowly when rous sarcoma virus-transformed chick embryo fibroblasts were grown in medium containing fetal bovine serum (fbs), and rapidly when chicken serum was employed. this difference reflected the low plasminogen and high inhibitor content of fbs. the inhibitors were found to be serum macromolecules that were precipitated with ammonium sulfate or ... | 1978 | 27528 |
| stimulation of tyrosine hydroxylase activity in cultured mouse neuroblastoma cells by monocarboxylic acids. | | 1978 | 27586 |
| abnormal cellular copper metabolism in the blotchy mouse. | defective copper metabolism was demonstrated in male mice bearing the blotchy (moblo/y) allele at the mottled locus on the x-chromosome. copper absorption from the gut was only 64% of that found in normal mice and hepatic copper levels were only 56% of the controls. ceruloplasmin and heart cytochrome c oxidase activities were normal, yet lysyl oxidase activity from cultured fibroblasts was only 45% of control levels. copper accumulated in fibroblasts cultured from these mutants to values that we ... | 1978 | 27591 |
| inhibitory effect of acetazolamide on the activity of acetyl coa carboxylase of mouse liver. | | 1978 | 27690 |
| characterisation of ribosomal proteins from hela and krebs ii mouse ascites tumor cells by different two-dimensional polyacrylamide gel electrophoresis techniques. | electrophoresis of ribosomal proteins according to kaltschmidt and wittmann, 1970a, b (ph 8.6/ph 4.5 urea system) yielded 29 proteins for the small subunits and 35 and 37 proteins for the large subunits of krebs ii ascites and hela ribosomes, respectively. analysis of the proteins according to a modified technique by mets and bogorad (1974) (ph 4.5/ph 8.6 sds system) revealed 28 and 29 proteins in the small subunits and 37 and 38 proteins in the large subunits of krebs ii ascites and hela riboso ... | 1978 | 27716 |
| the leeuwenhoek lecture, 1977. mouse teratocarcinoma and mouse embryo. | | 1978 | 27802 |
| some aspects of the h-2 system, the major histocompatibility system in the mouse. | | 1978 | 27811 |
| the effect of inhibitors of cellular rna synthesis on stimulation of mouse encephalomyocarditis virus reproduction by poliovirus in hela and mio cells. | inoculation of hela and mio cells with a guanidine-dependent mutant of poliovirus (gd-polio) in the absence of guanidine resulted in stimulation of mouse encephalomyocarditis (emc) virus reproduction. no stimulation was found to occur in the presence of actinomycin d, on pre-treatment of the cells with cordycepin, or on their uv irradiation or enucleation. in contrast to hela cells, the enhancement of emc virus reproduction by gd-polio in mio cells required no current synthesis of cellular rnas: ... | 1978 | 27964 |
| transmission of kyasanur forest disease virus by rhipicephalus haemaphysaloides ticks. | larvae of rhipicephalus haemaphysaloides were infected with kaysanur forest disease (kfd) virus by feeding on viraemic rodents and reared into next generation larvae. fed larvae, nymphs, unfed adults, fed adult males, and females after oviposition were found infected, while the larvae were found free from infection. nymphs and adults transmitted the infection by bite to rodents and rabbits respectively. the virus was also passed through a second rodent-tick cycle. adult ticks showed a titre of 3 ... | 1978 | 27975 |
| neuro-epithelial bodies in the lung of the rat and the mouse. | histochemical and ultrastructural examinations were performed on neuroepithelial bodies (n. e. b.) in the lung of the mouse and rat. the n. e. b. were identified as specialized groups of pale, columnar cells. they were located throughout the intrapulmonary airway. these cells displayed some special cytochemical properties also seen in the apud (amine precursors uptake and decarboxylation) endocrine system, such as cytoplasmic argyrophilia and the capability of selective uptake of amine precursor ... | 1977 | 28107 |
| glial fibrillary acidic protein in reaggregating and monolayer cultures of fetal mouse cerebral hemispheres. | | 1978 | 28168 |
| the anaerobic dechlorination of trichlorofluoromethane by rat liver preparations in vitro. | incubation of trichlorofluoromethane with a liver microsomal fraction and an nadph generating system under anaerobic conditions produced a metabolite dichlorofluoromethane, characterised by gas chromatography and mass spectrometry. the metabolic reaction was carried out by liver microsomes from the mouse, rabbit, hamster and rat and was increased by phenobarbitone pre-treatment. the formation of dichlorofluoromethane in vitro was enhanced by the addition of fmn, but partially inhibited by the pr ... | 1978 | 28182 |
| mammary tumor virus dna: a glucocorticoid-responsive transposable element. | | 1978 | 28200 |
| climbing behaviour induced by apomorphine in mice: a potential model for the detection of neuroleptic activity. | apomorphine and the putative dopamine agonist, 2-(n, n-dipropyl)-amino-5, 6-dihydroxytetralin induced dose-dependent climbing behaviour in the mouse which was measured in wire mesh lined cages as the percentage of time spent climbing in the 30 min period following the first climb and as the maximum time spent in a single climb throughout the drug effect. these these two measures were generally found to parallel excepting when the interacting agent caused muscular hypotonia. all potential interac ... | 1978 | 28233 |
| halothane-induced sleeping time in the mouse: its modification by benzodiazepines. | the conditions under which prolongation of halothane-induced sleeping time in the mouse may be used as a test for centrally acting drugs are described. the test can be recommended for its practical advantages over methods using barbiturates to induce hypnosis; due cognizance must be taken of a diurnal variation in the response of mice to halothane. to assess the usefulness of the test the effects of amphetamine, chlorpromazine, histamine, morphine, nikethamide, pentobarbitone and skf 525a have b ... | 1978 | 28236 |
| inhibition of allergic reactions by a new antiallergic drug, lc-6 (trans-2,3b,4,5,7,8b,9,10-octahydronaphthol1,2-c:5,6-c' dipyrazole). i. inhibition of the rat reaginic passive cutaneous anaphylaxis. | a new synthetic compound, lc-6, has been shown to inhibit the passive cutaneous anaphylaxis reactions induced in rats by mouse reaginic antibody. in this system, the ed50 was 35 mg/kg body weight of lc-6 administered per os. lc-6 prevented neither histamine skin reactions nor the reactions to histamine and other chemical mediators released by 48/80. therefore, its inhibitory activity is comparable to that of the model anti-allergic compound, disodium cromoglycate (dscg). in contrast to dscg, the ... | 1978 | 28295 |
| adrenergic enzymes in cultured mouse neuroblastoma: absence of detectable aromatic-l-amino-acid decarboxylase. | | 1978 | 28384 |
| concomitant elevation of tyrosine hydroxylase and dopamine beta-hydroxylase by cyclic amp in cultured mouse neuroblastoma cells. | | 1978 | 28385 |
| interaction between doxapram and pentobarbitone in the mouse. | | 1978 | 28408 |
| single-mouse urine collection and ph monitoring system. | a glass cage with minimal surface area was designed and used to house mice for 24-hour urine collections. an experiment was performed with a radio-labeled compound excreted in the urine to assess the collection efficiency of the cage. in this experiment 74.2 +/- 6.5% of the excreted radioactivity was recovered in the urine, with 25.8 +/- 6.5% found adhering to the cage surfaces. when a flow-through ph electrode, meter, and recorder were attached, the system provided a continuous ph versus time u ... | 1978 | 28449 |
| the interaction of phenoxybenzamine with the mouse brain opiate receptor. | | 1978 | 28470 |
| bone marrow transplantation in cancer therapy: inactivation by antibody and complement of tumor cells in mouse syngeneic marrow transplants. | | 1978 | 28530 |
| mechanism of interferon action. properties of an interferon-mediated ribonucleolytic activity from mouse l929 cells. | | 1978 | 28606 |
| glutathione depletion following inhalation anesthesia. | glutathione depletion following inhalation of halogenated anesthetics was investigated as a possible mechanism of toxic reactions associated with anesthesia. concentrations of reduced glutathione were measured in the blood, liver, lung and kidney of the mouse after anesthesia with enflurane, fluroxene, halothane, isoflurane, methoxyflurane, or trichloroethylene. the anesthetic had no effect on glutathione concentrations in tissues except when fluroxene was used. after two hours of fluroxene anes ... | 1978 | 28682 |
| deoxycytidine: a morphine antagonist. | deoxycytidine antagonizes the analgesic action of morphine in the rat, morphine-induced respiratory depression in the rabbit and mitigates withdrawal in the dependent mouse. administration of deoxycytidine does not precipitate the abstinence syndrome in dependent mice, a property shared by certain other endogenous morphine antagonsits. cytidine and thymidine closely related in structure to deoxycytidine, are interchangeable with saline as controls, indicating a high degree of specificity in the ... | 1978 | 28700 |
| esterase xxvii. purification and characterization of esterase-9a of mouse kidney. | esterase-9a, which appears electrophoretically as a triplet of the bands iii-50, iii-40 and iii-30, was isolated from the kidneys of male nmri-mice by isoelectrofocusing and refocusing followed by repeated molecular sieve chromography. the overall purification was approx. 250 fold and each of the three bands was isolated separately. the band of the triplet nearest to the cathode, iii-50, changed in vitro into the satellite bands iii-40 and iii-30 and, further, into the band iii-22 not observed b ... | 1978 | 28771 |
| status of t- and b-lymphocytes in long living cba--f1 (cba x c57bl/6) chimeras. | semiallogeneic chimeras were produced by injecting 3 x 10(7) spleen cells of mice cba (h--2k, mlsd) to lethally irradiated mice (cba x c57bl/6)f1. two days later recipients were given cyclophosphamide (cp), 2 mg per mouse, to prevent death of graft versus host reaction (gvhr). for 1.5--2 months after the creation of chimerism in 23 of 26 mice under study all cells producing antibodies to srbc were represented by donor cells of h-2 phenotype; 3 mice were partial chimeras. spontaneous blast transf ... | 1978 | 28802 |
| effect of autonomic agents on the amount of androgen-dependent granules in convoluted tubular cells of the mouse submandibular gland. | the convoluted tubular cells of the male mouse submandibular gland contain many serous-like granules in their apical cytoplasm. the autonomic regulation of the secretory process of the contents of these granules was studied by the following two methods: (1) immunochemical method using an antiserum specific to the granular components; and (2) histometric observations using light and electron microscopes. the results obtained by these two methods were well in agreement. when male mice were adminis ... | 1978 | 28825 |
| increase by calcium in production of interferon by l929 cells induced with polyriboinosinate-polyribocytidylate complex. | calcium chloride (5 to 20 mm) potentiated interferon production induced by rin:rcn in l929 mouse fibroblasts up to a thousand-fold. higher concentrations of calcium (20 to 65 mm) mixed with rin:rcn were associated with increased cytotoxicity and a more acidic medium, but were effective in enhancing interferon production if preparations were adjusted to a uniform ph. although calcium increased cellular binding of 3h-rcn:rin, only a partial correlation between binding and interferon production was ... | 1978 | 29078 |
| poly (a) polymerase activity in l cells following encephalomyocarditis virus infection. | poly (a) polymerase activity has been measured in crude cytoplasmic extracts of mouse l cells infected with encephalomyocarditis (emc) virus. after infection there is first a decrease in enzyme activity followed by an increase which itself precedes detectable virus rna and protein synthesis. the activity of the enzyme then declines before the release of mature virions and cell death take place. the early inhibition of poly (a) polymerase activity is correlated with the virus-induced shut-off of ... | 1978 | 29079 |
| the isolation of japanese encephalitis virus from taiwan mosquitoes by mosquito cell cultures and mouse inoculation. | | 1978 | 29127 |
| muscarinic cholinergic activation of mouse spleen cells cytotoxic to tumor cells in vitro. | carbamylcholine, acting via a pharmacologically specific receptor, had the ability to activate effector populations of spleen cells from female balb/cfc3h and balb/c mice; those cell populations were then significantly reactive in vitro against syngeneic tumor target cells but were only minimally reactive to normal syngeneic target tissues. the induced reactivity was inhibited by the muscarinic cholinergic antagonists atropine, scopolamine, and isopropamide, but not by the nicotinic antagonist d ... | 1978 | 29133 |
| experiments to increase the selectivity of tumor chemotherapy by means of in vivo activation of transport forms of cancerostatics by exogenous enzymes. | a significant tumor damaging effect (growth inhibition) on transplanted syngeneic sarcoma in mouse was obtained by means of ph-dependent activation of a transport form of a cancerostatic drug by an enzyme foreign to the organism. this effect was achieved by combined administration of 8-0-(alpha-l-arabinofuranosyl)beta-peltatin-a as a transport form of beta-peltatin-a and the exogenous enzyme alpha-l-arabinofuranosidase from aspergillus niger and additional increase of the acidity of the tumor by ... | 1978 | 29245 |
| enzymatic regulation of the concentration of cyclic gmp in mouse brain. | | 1978 | 29258 |
| effect of mouse age on the ability of hematopoietic stem cells to interact with thymus cells. | the effect of the thymus cells of the c57bl/6 mice on the colony forming ability of the stem hemopoietic cells of the embryonic liver and bone marrow of young (3 months) and old (2 years) mice was studied their joint transplantation into the mice (cbaxxc57bl/6) f1. the stimulating effect of the thymus cells on the colony forming ability of the stem hemopoietic cells of different age depends both on the dose of the stem hemopoietic cells of embryonic liver and the dose of t-lymphocytes. a suggest ... | 1978 | 29265 |
| retained inhibition of the beta receptor by propranolol after preincubation and washout. | mouse thymus cells possess a b2-adrenergic receptor and, when centrifuged through a solution of propanolol at 5 degrees and washed three times, will produce reduced amounts of cyclic amp during a 5 minute challenge with isoproterenol at room temperature. depending on the concentration and nature of the antagonist, the retained inhibition may be greater or less than that seen when it is added with the agonist. this retained inhibition is readily reversed at 37 degrees even in the absence of the a ... | 1978 | 29326 |
| interspecies interactions of arboviruses. iii. competition for virus envelope antigens in mixed getah and sindbis virus populations. | interactions of getah and sindbis virus populations in mixed infections of 5 tissue systems were competitive or neutral. the type of interaction is regulated by the host cell and virus population density. on reproduction in mouse brains and spev cell cultures the mixed population exhibited antigenic markers of getah virus; in syrian hamster kidney and chick embryo cell cultures those of sindbis virus; and in chinese hamster kidney cell cultures the antigenic markers of both viruses. mixed popula ... | 1978 | 29465 |
| correlation between dna repair of embryonic fibroblasts and different life span of 3 inbred mouse strains. | primary mouse fibroblast cultures were established from 10 day old embryos of 3 inbred strains with a genetically determined different life expectancy. the capacity for unscheduled dna synthesis following u.v. irradiation was studied in these cells at various passage levels. the mouse fibroblasts show considerable repair synthesis corresponding to the duration of exposure time. the capacity for induction of unscheduled dna synthesis was different in the cells of each strain and correlated to the ... | 1978 | 29506 |
| tissue sterility in uneviscerated carcasses. | sheep muscle tissue removed aseptically from control carcasses, from uneviscerated carcasses held at 20 degrees c for 24 h, and from carcasses of sheep subjected to stress before slaughter was examined for the presence of bacteria. all samples from a total of 68 carcasses were sterile. whole-body autoradiography of mouse carcasses showed that 14c-labeled fixed bacteria injected after death remained in the lumen of the intestine. live bacteria did not penetrate the mucosal surface until the tissu ... | 1978 | 29564 |
| alkaline deoxyribonuclease activity in mouse teratocarcinoma cells: variation of enzyme levels during differentiation. | | 1978 | 29624 |
| localization and significance of gamma-glutamyltranspeptidase in normal and neoplastic mouse skin. | | 1978 | 29703 |
| the effect of ketosis induced by medium chain triglycerides on intracellular ph of mouse brain. | | 1978 | 29752 |
| factors regulating development of an embryonic mouse sympathetic ganglion. | | 1978 | 29814 |
| long-term effect of ph on b-cell function in isolated islets of langerhans in tissue culture. | collagenase isolated mouse pancreatic islets were maintained in tissue culture for up to 5 months in a culture medium buffered with hepes and the ph varying between 6.8 and 7.6. the amount of insulin released into the medium and the insulin response to glucose and glucose plus theophylline were measured during the culture period. it was found that islets cultured at ph 7.2 maintained the ability to release insulin into the medium for at least 5 months, which was longer than islets cultured at th ... | 1978 | 29815 |
| t-lymphocyte heterogeneity in the rat: separation of functional subpopulations using a monoclonal antibody. | w3/25 antibody is the monoclonal product of a hybrid cell resulting from the fusion of a mouse myeloma cell line with spleen cells from a mouse immunized with rat thymocytes. pure clones have been derived, and segregants free of parental myeloma chains have been isolated. previous studies have shown that this antibody recognizes a subpopulation of t cells among rat thoracic duct lymphocytes. in the work reported here, three t-cell functions were assayed after separating rat thoracic duct lymphoc ... | 1978 | 29936 |
| in vitro studies on lymphocyte differentiation. i. long term in vitro culture of cells giving rise to functional lymphocytes in irradiated mice. | in vitro cultures of mouse bone marrow cells, maintained for periods up to 7 wk, were shown to contain cells able to repopulate irradiated hosts with t and b lymphocytes. the lymphocytes were fully functional and there did not appear to be any gross restriction of their receptor repertoire. the cultured cells reconstituted irradiated semiallogenic hosts without evidence of graft-versus-host disease, suggesting that culture of donor marrow might be a useful preliminary to transplantation when tis ... | 1978 | 29938 |
| guanylate cyclases in cns: enzymatic characteristics of soluble and particulate enzymes from mouse cerebellum and retina. | | 1978 | 29943 |
| mouse brain tyrosine hydroxylase and glutamic acid decarboxylase following treatment with adrenocorticotrophic hormone, vasopressin or corticosterone. | | 1978 | 29946 |
| effect of starvation, nutriment replacement, and hypothyroidism on in vitro hepatic t4 to t3 conversion in the rat. | to evaluate the effect of starvation, oral and i.v. nutriments, and hypothyroidism on the peripheral conversion of thyroxine (t4) to 3,3', 5-triiodothyronine (t3) in the rat and mouse, an in vitro system for assessing t4 conversion to t3 by fresh liver homogenates was used. a 2-day starvation in the rat reduced hepatic t3 generation from t4 by 47% +/- 3.5% (mean +/- se) in six separate experiments and also impaired the metabolism of 125i-r-t3. administration of carbohydrate (cho) and amino acids ... | 1978 | 30020 |
| role of neuroleptic agents on mouse pulmonary cyclic nucleotide systems. | the norepinephrine (ne)-induced accumulation of cyclic amp in incubated tissue slices of mouse lung was inhibited by chlorpromazine (cpz) and to a lesser extent by haloperidol. in particulate lung fractions both agents blocked dopamine-sensitive adenylate cyclase to a greater degree than the ne-responsive enzyme. again cpz was more potent than haloperidol. acute injections (1/2--8h) of the neuroleptics usually resulted in lower steady state levels of pulmonary cyclic amp and cyclic gmp following ... | 1978 | 30096 |
| mouse peyer's patches contain t cells capable of inducing the graft-versus-host reaction (gvhr). | the potential of cells from the peyer's patches (pp) of normal adult dba/2 tru mice (dba/2) to induce a graft-versus-host reaction when injected into (c57bl/6 tru x dba/2 tru)f1 hybrid (b6d2f1) mice was studied. the injection of 10(6) to 10(7) dba/2 pp or spleen cells i.p. into neonatal f1 mice produced a striking splenomegaly. comparable doses of parental pp or spleen cells injected into a rear footpad of adult f1 mice also induced a marked enlargement of the draining popliteal lymph node. in ... | 1978 | 30192 |
| effect of irradiated mouse spleen cells on severity of graft-versus-host disease. | | 1978 | 30193 |
| variations in biological properties of geographic strains of western equine encephalomyelitis virus before and after passage in culex tarsalis and culiseta melanura. | western equine encephalomyelitis (wee) virus strains from the eastern and western united states were compared using two biological markers: antigenic properties measured by cross complement fixation (cf) tests and the size of plaques produced in vero cell cultures. slight antigenic differences were discernible on a geographic basis, and all of the virus strains from the west produced significantly larger plaques than the eastern isolates. one virus strain from each region was serially passaged i ... | 1978 | 30265 |
| effect of acute and chronic alcoholization upon the activity of clobazam in mouse (author's transl). | | 1978 | 30385 |
| experimental analysis of interference between alpha-adrenergic stimulants with anti-hypertensive activity and the narcotic activity of pentobarbital. | the interactions of 3 classical alpha-adrenergic antihypertensives of prevalently central type (st 155 or clonidine st 600; br 750 or guanabenz) with the narcotic effects of pentobarbital have been investigated in the mus musculus. interferences between convulsant drugs and spontaneous and coordinatory motor activity were also analysed in comparative studies. | 1978 | 30418 |
| comparative physiological disposition of ellipticine in several animal species after intravenous administration. | the physiological dispositon of ellipticine (nsc 71795) has been studied in the mouse, rat, dog and monkey after administration of [1-14c]ellipticine at 6 mg/kg iv (3 mg/kg to monkey). ellipticine was very rapidly distributed from the blood of all species and was deposited in tissues. the rate of elimination of ellipticine from blood was species-dependent, half-times ranging from 22 min in mouse to 210 min in rat, and probably reflected the rate of metabolism of the drug. the rate of elimination ... | 1978 | 30602 |
| isolation of pure igg1, igg2a and igg2b immunoglobulins from mouse serum using protein a-sepharose. | | 1978 | 30693 |
| protective ability of salmonella ribosomal protein and rna in inbred mice. | ribosomal vaccines prepared from salmonella typhimurium were effective immunogens in a/j, c3h/hedub, and c3h/hej mice. purified ribosomal components were also tested as immunogens in the inbred mice. protein isolated from a salmonella ribosomal fraction could protect all three mouse strains. although purified rna was shown to be protective for a/j and c3h/hedub mice, it was not protective for c3h/hej mice. protective immunity could be induced in a/j and c3h/hedub mice by various immunostimulants ... | 1978 | 30697 |
| platelet derived growth factor(s) for a hormone-responsive rat mammary tumor cell line. | mammary tumor cell growth factor(s) has been identified in extracts of platelets from both male and female rats, as well as in extracts prepared from pooled outdated human platelets. when assayed by the growth promotion of mtw9/pl rat mammary tumor cells in culture, platelet extracts alone were able to support growth 50--75% as well as whole serum. the mitogenic activity from crude human platelet lysates was shown to be trypsin sensitive, relatively stable to extremes of ph, labile to heat treat ... | 1978 | 30782 |
| the beta-adrenergic receptor of newborn mouse skin. | binding of the potent beta-adrenergic antagonist [125i]iodohydroxybenzylpindolol ([125i]ihyp) to particulate preparations from newborn mouse skin was characterized. a number of criteria were used to establish that binding occurred to specific, high affinity beta-adrenergic receptors in the skin preparations. thus specific binding (that displaced by 10 micrometer concentrations of the beta-adrenergic antagonist (-)propranolol) reached equilibrium in 15--20 min, was saturable (ligand concentration ... | 1978 | 30802 |
| multiple forms of alpha-galactosidase of the mouse and their use as a cell marker in tumorigenesis. | | 1978 | 30845 |
| physico-chemical properties of mouse hepatitis virus (mhv-2) grown on dbt cell culture. | some properties of a strain of mouse hepatitis virus, mhv-2, grown on dbt cells were determined using a plaque assay on the cells. viral growth was not inhibited by the presence of actinomycin d or 5-iodo-2-deoxyuridine. mhv-2 was completely inactivated by ether, chloroform, sodium deoxycholate or beta-propiolactone, but showed a moderate resistance to trypsin. heating at 56 c for 30 min did not completely abolish the virus infectivity. the virus was stable after heating at 50 c for 15 min in 1m ... | 1978 | 30881 |
| methadone uptake by l5178y mouse leukemic cells. | | 1978 | 30891 |
| [manipulated hyperacidification of autochthonous tumors]. | measurement on 3,4-benzopyrene- and methylcholanthrene-induced tumours of the rat (and mouse) showed that the multiple established manipulated hyperacidification of transplantation tumours to values about ph 6 is possible also on autochthonous tumours. | 1978 | 30927 |
| [effect of different temperature fractions of rna on allogenic inhibition of hematopoietic stem cells of mouse bone marrow and embryonal liver]. | in the system of non-syngeneic transfer of stem hemopoietic cells, the preliminary incubation of the cells of bone marrow or embryonic liver of the c57bl mice with different temperature rna fractions isolated from the spleen of (cbaxc57bl) f1 was shown to lead to the complete or partial restoration of the colony forming ability of the donor cells. the 63 degrees rna fraction was shown to have the greatest restorative effect. | 1978 | 30930 |
| the effects of fractions (chalones) obtained from lymphoid organs on the immune response in vivo. | aqueous extracts of calf and pig lymphoid organs were prepared and fractionated by means of gel filtration, ion exchange chromatography, and isoelectric focusing. these fractions, which had been previously assessed on mitogen-stimulated mouse spleen lymphocytes and other cells in vitro, were tested for their in vivo activity on humoral (haemolytic pfc in mice) and on cell-mediated immunity (skin allograft survival in mice, lymph node weight assay in rats, and systemic gvh-reaction in mice). none ... | 1978 | 31079 |
| [effect of conditions of cultivation on quantitative assessment of the viability of an organism's tissues]. | viability of mouse kidney tissue explants as estimated quantitatively according to their growth in plasmafree culture depends on some factors connected with cultivation conditions. methods of serum preparation, ph of the medium, as well as peculiarities of tissue explants distribution in cultivation, their size and fastness of attachment to the substrate proved to influence the quantitative estimation of the tissue explants viability. | 1978 | 31213 |
| a technique for the measurement of breakage and repair of dna alkylated in vivo. | the alkaline elution technique has been adapted for use in the assessment of dna damage induced in the livers and lungs of mice after administration of an alkylating agent, methylemthanesulfonat (mms). at 4 h after administration of mms, damage ot dna was readily demonstrable; the damage was repaired in liver by 24 h. the lung, particularly of the a/j mouse, exhibited an increased alkaline elution rate when compared to c57bl/6j, and repair was not entirely complete (as judged from the rate of al ... | 1978 | 31244 |
| ultrastructural findings in the mouse exocrine pancreas during graft-versus host reaction. | 12- to 36-hours-old newborn cba mice were intraperitoneally injected each with 10 x 10(6) allogeneic spleen cells of adult c57bl mice. control mice received syngeneic spleen cells of adult cba mice either in the same way or remained untreated. the animals were killed 1, 3, 7, 14 or 21 days after the spleen cell injection. the pancreas was studied histologically and electron microscopically by common methods. together with an interstitial lymphohistiocytic infiltration of the pancreas different a ... | 1978 | 31294 |
| potentiation of hyperphagia and relief of hypothermia in the genetically obese mouse (genotype, ob/ob) by alpha-methyl tyrosine. | dl-alpha-methyl-p-tyrosine methyl ester hydrochloride affected the hyperphagia and hypothermia characteristic of the genetically obese mouse (genotype, ob/ob) throughout an experimental period of 5 days. intraperitoneal injections of 100 mg/kg body weight, daily, resulted in a significant increase in the average daily food consumption by 60 per cent, already elevated 35 per cent above that of lean litter-mates. the drug, administered at the same dose, caused a similar percentage elevation of foo ... | 1978 | 31336 |
| regulation of glycosphingolipid metabolism in mouse neuroblastoma and glioma cell lines. comparison of glioma (oligodendroglioma-like) with neutroblastoma cell lines. | | 1979 | 31364 |
| the mouse gut t lymphocyte, a novel type of t cell. nature, origin, and traffic in mice in normal and graft-versus-host conditions. | lymphocytes of the mouse intestinal mucosa, identified in tissue sections or purified suspensions of intraepithelial lymphocytes as t cells (gut t lymphocytes [gtl]), were studied in normal mice or in beige mice (the equivalent of the chediak-higashi syndrome in man, characterized by giant granules in various cell types, including mast cells). mice were studied in normal or in germ-free conditions, or during a graft versus host (gvh) reaction resulting from the injection of parental thymocytes i ... | 1978 | 31410 |
| comparison of adenylate cyclase activity and in vitro secretion in the parotid and sublingual glands of the mouse. | 1. adenylate cyclase (ec 4.6.1.1) activity has been determined in the parotid and sublingual glands of the mouse. optimal activity of the enzyme was obtained at a mg2+-concentration of 8 mm at ph 8.2, using amp-pnp as the substrate. 2. cyclic amp degradation during the adenylate cyclase assay was relatively high in both the homogenate and the 40,000 g pellet-fraction of the glands. theophylline was effective in inhibiting this degradation only in the parotid hemogenate, whereas isobutylmethylxan ... | 1978 | 31465 |
| adrenergic and 'non-adrenergic' components in the contractile response of the vas deferens to a single indirect stimulus. | 1. the mechanical response of the longitudinal smooth muscle of the rat vas deferens to stimulation of its motor nerves by a single pulse has been examined. the motor nerves were stimulated in vivo via the spinal outflows in the pithed rat or in vitro by field stimulation. 2. the contraction in the whole vas consisted of two components, an initial, rapid, brief contraction reaching a maximum at 300 msec and a second, slower and more prolonged contraction reaching its maximum at 600 msec. when th ... | 1978 | 31466 |
| the newborn mouse as a model for study of the effects of hormonal steroids in the young. | | 1978 | 31596 |
| california encephalitis group viruses isolated from mosquitoes collected in southern and arctic norway. | three virus strains serologically related to the california encephalitis group (bunyaviridae) of arboviruses were isolated from 7331 mosquitoes collected in norway in june-august 1975. two of the isolates (s 548 and s 618) seemed to be closely related and the third, s 568, more distantly related by serological techniques to tahyna virus. viruses were found in the mosquito species aedes sticticus, a. diantaeus and a. hexodontus colllected (in order) from oyern (59 degrees n, 11 degrees 12' e), tr ... | 1978 | 31761 |
| the in vitro interaction of trypanosoma cruzi bloodstream forms and mouse peritoneal macrophages. | the uptake and further development of bloodstream forms from t. cruzi y and cl strains in mouse peritoneal macrophages have been investigated. parasites from the y strain (which present predominance of slender forms) are 20 to 30-fold more infective to macrophages than those from cl strain in which stout forms highly predominate. a complete amastigote-trypomastigote cycle is observed in normal or thioglycollate-induced macrophages infected with parasites from both strains.--opsonization signific ... | 1978 | 31774 |
| effect of the mouse mutants testicular feminization and sex reversal on hormone-mediated induction and repression of enzymes. | the mouse mutants testicular feminization and sex reversal have been used to investigate hormone-mediated induction and repression of enzymes. tfm/y animals were already known to be androgen insensitive, rendering the androgen-inducible enzymes adh and beta-glucuronidase noninducible because of an inherited deficiency of a cytosol androgen-receptor complex. the animals display female secondary sexual characteristics. sxr/+,xx animals display male primary and secondary sexual characteristics with ... | 1978 | 31863 |
| effects of glucagon and insulin on fatty acid synthesis and glycogen degradation in the perfused liver of normal and genetically obese (ob/ob) mice. | 1. rapid effects of hormones on glycogen metabolism and fatty acid synthesis in the perfused liver of the mouse were studied. 2. in perfusions lasting 2h, of livers from normal mice, glucagon in successive doses, each producing concentrations of 10(-10) or 10(-9)m, inhibited fatty acid and cholesterol synthesis. in perfusions lasting 40--50 min, in which medium was not recycled, inhibition of fatty acid synthesis was only observed with glucagon at concentrations greater than 10(-9)m. this concen ... | 1978 | 31866 |
| phenotypic exclusion in mouse melanoma-rat hepatoma hybrid cells: pigment and albumin production are not reexpressed simultaneously. | hybridization of cells of defined and different histotypes has been carried out to investigate whether the expression (or reexpression) of parental functions is mutually exclusive, as is expected if the generally assumed rule of discreteness of differentiation applies to hybrid cells. a cross of pigmented mouse melanoma cells and albumin-producing rat hepatoma cells gave rise to hybrids containing essentially one set of chromosomes from each parent and producing neither melanin nor albumin. cell ... | 1978 | 31979 |
| the xvith international symposium on laboratory animals. hrubá skála. april 1978. section ii: the nude mouse. | | 1978 | 32081 |
| abstracts of papers presented at the workshop on molecular genetics of the mouse september 4-6, 1978 at the institute of ecology and genetics university of aarhus, denmark. | | 1978 | 32153 |
| toxicity of sodium ascorbate and alloxan monohydrate to 3t3 mouse cells [proceedings]. | | 1978 | 32336 |
| characterization of rauscher murine leukemia virus envelope glycoprotein receptor in membranes from murine fibroblasts. | plasma membrane preparations from ka31 (mouse) cells contained receptors for the binding of rauscher murine leukemia virus (r-mulv) envelope glycoprotein, gp70. this binding was demonstrated by gel filtration of a mixture of the microsomal fraction of the cells and 125i-labeled gp70. a rapid and convenient assay was developed to measure the complex formation between the membrane receptors and gp70 involving specific precipitation of the complex by 3 to 4% polyethylene glycol. the complex formati ... | 1978 | 32403 |
| purification of a nontoxic phospholipase a2 from the venom of indian krait (bungarus caeruleus). | a nontoxic phospholipase a2 was purified from the venom of indian krait (bungarus caeruleus) by a four-step procedure involving electrophoresis, gel filtration and ion-exchange chromatography. the recovery of the enzyme activity was 37% and the purified preparation was 38 times as active as the crude venom. the purified enzyme had a molecular weight of 12,500 and the optimum ph of 7.2. the enzyme showed higher specificity toward phosphatidylethanolamine than phosphatidylcholine. the preparation ... | 1978 | 32414 |
| electrophysiological examination of transmitter release in non-quantal form in the mouse diaphragm and the activity of membrane atp-ase. | the subsynaptic area of mouse diaphragm fibres was hyperpolarized by 1--2 mv during local curarization of the junctional zone in the presence of the reversible anticholinesteraze prostigmine (6 x 10(-6) m), or after treatment of the muscle with organophosphate cholinesterase inhibitor soman. in a solution containing 5 mm k+ the mean hyperpolarization was 1.1 +/- 0.27 mv at mean resting potential--70 mv. after adding 2 x 10(-5) m ouabain the hyperpolarization increased to 1.5 +/- 0.25 mv. removal ... | 1978 | 32568 |
| dopamine-receptor binding and adenylate-cyclase activity in mouse striatal tissue in the supersensitivity phase after neuroleptic treatment. | | 1978 | 32570 |
| ontogeny of suppressor cells. ii. suppression of graft-versus-host and mixed leukocyte culture responses by embryonic cells. | ontogenic development of suppressor cells was studied in mouse embryos. it was found that liver cells of embryos at various stages of gestation were capable of interfering with the mixed leukocyte culture (mlc) reaction. the mlc reaction was also inhibited by embryonic spleen and thymus cells. however, the latter were inhibitory only when originating in 16- and 17-day-old embryos. embryonic liver cells were also found to interfere with the graft-versus-host (gvh) response induced in neonatal (ba ... | 1978 | 32650 |
| studies on trypanosoma (nannomonas) congolense. i. on the morphological appearance of the parasite in the mouse. | the pleomorphism of bloodstream trypanosoma (nannomonas) congolense was studied during the course of the first parasitaemic wave in mice using cloned and uncloned derivatives of three recent field isolates. the different morphological types were identified using the criteria described by godfrey (1960). it was found that at any point of parasitaemia there were several morphological types of the parasite present, ranging from short to long forms. in the rising phase of parasitaemia, the short for ... | 1978 | 32752 |
| relationship between sperm concentration and polyspermy in intact and zona-free mouse eggs inseminated in vitro. | intact and zona-free mouse eggs were cultured with preincubated (capacitated) spermatozoa for 1 or 4 hr. high proportions of eggs (84%--100%), examined either 1 or 4 hr after insemination, were undergoing fertilization in the intact and zona-free eggs in sperm concentration from 25--800 x 10(3) sperm/ml. the average number of spermatozoa attached to the zona pellucida and to the vitellus was only slightly increased as the sperm concentration increased. polyspermy was increased from 25--200 x 10( ... | 1978 | 32851 |
| [metabolic activation of n-methyl-n-nitrosourea in normal and tumor cells]. | n-methyl-n-nitrosourea is metabolised by mouse liver microsomes yielding highly reactive product(s) capable of alkylating cellular macromolecules. based on cofactor requirement (nadph, oxygen), inhibition (nan3) and induction by phenobarbital, 3,4-benz(a)pyrene, and mnu, the reaction is cytochrome p-450-dependent. analysis of the kinetics and dose dependence of alkylation in vivo and in tissue homogenates confirms the fact that mnu must undergo metabolic activation in vivo. | 1978 | 32924 |