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[periosteal new bone formation in the horse induced by trauma. (author's transl)].the radiological interpretation of periosteal new bone formation in the horse induced by trauma is described and illustrated by some casereports. the better the margins of the new bone can be defined the lesser the activity. the greater the density of the new bone, the longer the duration of time since injury.1979112712
evaluation of the antiserum agar method for the serogroup identification of neisseria meningitidis.the antiserum agar method (asa), which is based on the formation of immunoprecipitates around bacterial growth on agar containing meningococcal hyperimmune horse serum, was evaluated for serogroup identification of neisseria meningitidis. four hundred meningococcal stains were serogrouped by asa employing horse antisera to serogroups a, b, c, y, w135, z, and 29e and compared to serogroup identification by bacterial slide agglutination (ba) employing rabbit antisera. overall, there was 95% agreem ...1979113070
androgen and estrogen metabolism in male rhesus monkeys.using constant infusions of 3h-labeled androgens and 14c-labeled estrogens, the dynamics of androgen and estrogen metabolism have been studied in male rhesus monkeys. the mean (+/-) mcr, as measured in whole blood, for testosterone (t; 140 +/- 10 liters/day) was significantly smaller than the mcrs for androstenedione (a), estrone (e1), and estradiol (e2; 650 +/- 8, 1010 +/- 160, and 500 +/- 20 liters/day, respectively). these findings are compatible with the specific globulin binding of t in thi ...1979113203
interaction of mycoplasma pneumoniae with human lung fibroblasts: characterization of the in vitro model.the interaction of pathogenic mycoplasma pneumoniae and host cells was studied in cell cultures of mrc-5 human lung fibroblasts. a comparison of results obtained with fibroblasts in a monolayer format and with hamster tracheal explant cultures indicated that the former can bind significantly larger numbers of mycoplasmas. in addition, the attachment was 96% specific, that is, mediated through a neuraminidase-sensitive receptor on the host cell. uptake of mycoplasmas was directly related to the n ...1979113348
scanning electron microscopy of ciliary zones of the ciliate protozoa in the large intestine of the horse.the surface structure of the ciliary zone in 13 species of ciliates found in the large intestine of the horse was observed by scanning electron microscopy. in holophryoides ovalis many fine depressions considered to be a result of phagocytosis or pinocytosis in the naked cytostome were noticed. in blepharocorys spp. a distinct section was present between the portion with cilia and that without cilia. it was not present, however, in some species of the family buetschliidae, such as bundleia postc ...1979113521
[stimulation of interferon production].data on the effect of some factors on interferon production in vitro are presented the kinetics of interferon synthesis in response to superinduction was similar to the respective curve of the effect of uv-radiation on the cell. possible similarity in the effect of these factors on the mechanisms controlling interferon production is noted. an increase in interferon synthesis under the effect of ascorbic acid in cells of chick embryo fibroblast and l-929 was found. combined use of the inductors p ...1979114105
the presence of precipitating antibodies in the sera of horses with chronic obstructive pulmonary disease (copd).the sera of horses affected and not affected with chronic obstructive pulmonary disease (copd) were examined for precipitins to micropolyspora faeni and aspergillus fumigatus. precipitins to both antigens were not restricted to copd cases but occurred more frequently in animals affected with copd. many animals without detectable precipitins responded clinically to inhalation challenge with these antigens.1979114387
growth enhancement and serum replacement in cloning of murine mastocytoma and granulocyte/macrophage precursor cells: two distinct activities present in hemolysates.in the present study we investigated the serum replacing and enhancing activities of erythrocyte lysates, obtained from different animal species, on the growth of murine mastocytoma cells and normal granulocyte/macrophage precursor cells (cfu-c) present in bone marrow. the soft agar technique for cloning hemopoietic cells in vitro was used to quantitate the growth of these cells in culture. rat, rabbit, guinea pig, mouse and sheep hemolysates were tested for their capacity to replace or to enhan ...1979114402
aromatase inhibitors and their use in controlling oestrogen-dependent processes. 1979114710
[isolation and properties of highly purified c1. botulinum toxin type e].a new method of isolation of highly purified cl. botulinum toxin of e type from the cultural fluid of strain 188 centrifugates was developed. the method allows to isolate the toxin both in a precursor and in activated forms with a yield of 10--15%. the method includes fractionation by ammonium sulfate, ultrafiltration and subsequent column chromatography on deae-cellulose, sephadex g-200 and deae-sephadex a-50. the preparations were found homogeneous during polyacrylamide gel electrophoresis and ...1979115504
long-term culture of canine peripheral blood monocytes in vitro.various cultural conditions were assessed for their ability to maintain canine peripheral blood monocytes in vitro. approximately ten days after incubation of peripheral blood leukocytes in earle's minimum essential medium supplemented with homologous red cell lysates and normal horse serum, virtually a pure macrophage culture was obtained which could then be maintained for about two months. this culture was judged to be pure by surface marker analysis and their phagocytic activity. the number o ...1979115559
epithelial or dandruff allergen for the diagnosis of dog allergy by rast?when used for screening by the rast technique, dog epithelium provided too few positive results (29.2%) compared with dog dandruff (58.8%), cat epithelium (53.6%) and horse dandruff (52.2%). however, dog dandruff can provide an unexpectedly high incidence of positive rast results (45.2%) in sera from patients with an allergic history to cat or horse, but not to dog. dog dandruff allergens were found to cross-react with some cat or horse serum proteins in about 25% of such patients. we conclude t ...1979115618
inhibition of the growth of neisseria meningitidis by reduced ferritin and other iron-binding agents.serogroups of n. meningitidis were characterized as virulent or avirulent according to their capacity to establish meningococcal infection in mice. an agar plate diffusion technique demonstrated that iron had a definite growth-supporting role for both of these meningococcal types. the avirulent strains could use ionic or chelated iron as well as the virulent strains. iron-reversible growth inhibition occurred to the same extent for both bacterial types in the presence of the synthetic iron-chela ...1979115792
stimulation of prostaglandin synthetase activity in rat granulosa cells by gonadotropins in vivo. 1979116319
[immunochemical characterization of rabies virus: determination of precipitable antigens].immunodiffusion and immuno-electrophoresis techniques were used to detect rabies precipitable antigens. the passive haemagglutination test with group "o" human red cells treated with tannic acid, was used as control test. the study was done with brain suspensions of rabies virus infected mice (cvs strain). mouse and horse rabies antisera were used. the analysis of the results of both immunochemical assays showed the presence of two specific antigenic fractions of rabies virus. the same two fract ...1978116328
manufacture of antithymocyte globulin (atgam) for clinical trials.methods are described for preparing large amounts of horse anti-human thymocyte globulin (atg, atgam; the upjohn company) for clinical use. these methods have been used since 1968 to provide material for clinical trials. characteristics of 40 lots of atg are summarized.1979116400
principles of therapy. 1979116413
clonal analysis of mammalian cell cultures persistently infected with japanese encephalitis virus.more than 200 cells were cloned from populations of mammalian cells persistently infected with japanese encephalitis virus. only four cloned cultures contained cells that had viral antigen measurable by immunofluorescence and that released infectious virus, yet all clones harbored virus-specific rna. superinfection of cloned cells with wild-type japanese encephalitis virus did not produce cytopathic effects, but resulted in production of viral antigen and infectious virus in formerly nonproducin ...1979117119
effects of influenza, mumps, and western equine encephalitis viruses on fetal rhesus monkeys (macaca mulatta).pregnant rhesus monkeys were infected via instillation of influenza, mumps and western equine encephalomyelitis viruses respectively into the amniotic sacs at approximately 90 days gestation to determine if fetal infections would occur. virus was recovered from fetal tissues after seven days in 100% of the exposed animals. thus, the viruses are capable of causing fetal infection. rhesus monkey fetuses were inoculated with influenza, mumps and wee viruses by the direct intracerebral route at appr ...1979117564
fetal mortality and malformations associated with experimental infections of western equine encephalomyelitis vaccine virus in rhesus monkeys (macaca mulatta).pregnant rhesus monkeys were infected via installation of western equine encephalomyelitis (wee) vaccine virus into the amniotic sacs at 50 and 80 days gestation to determine if the resulting infections would produce fetal mortality or fetal malformations, particularly within the central nervous system. of those receiving virus at 50 days gestation, 13 of 18 fetuses were aborted or dead in utero at time of caesarean section; 2 of 18 were malformed (hydrocephalus and polyarthrosis); and 3 of 18 w ...1979117565
preparation of protoplasts and whole cell ghosts from mycobacterium smegmatis.cell wall-deficient forms of mycobacterium smegmatis were produced in growth medium containing d-cycloserine and horse serum. these cells were transformed into protoplasts with edta and lysozyme. subsequent lysis by nucleases followed by osmotic shock produced membrane vesicles (whole cell ghosts).1979119034
biochemical and antigenic comparison of the envelope glycoproteins of venezuelan equine encephalomyelitis virus strains.pulse-chase experiments after synchronous initiation of translation indicate that the larger venzuelan equine encephalomyelitis (vee) virus membrane glycoprotein e2, is derived by proteolytic cleavage of the precursor, pe2. the structural proteins of vee virus strains representing each of the antigenic subtypes and varieties have been compared by discontinuous sds-polyacrylamide gel electrophoresis. nucleocapsid proteins of all isolates were similar in size (mol. wt. 35 to 36 x 10(3). the mol. w ...1979119035
tetracycline-mediated photodynamic inactivation of animal viruses.demethylchlortetracycline (dmct), doxycycline and, to a lesser extent, chlortetracycline were capable of mediating the in vitro photoinactivation of venezuelan equine encephalitis (vee) virus. other tetracyclines tested were found to be inactive in this respect. however, no correlation between chemical structure and photosensitizing activity could be established. the photoinactivation of vee virus by dmct proceeds through a photodynamic mechanism as shown by the absolute requirement of o2 for th ...1979120411
factors affecting the in vitro culture of plasmodium falciparum and plasmodium knowlesi.plasmodium falciparum and plasmodium knowlesi have been established in continuous culture using the basic method of trager & jensen. various parameters of the culture system have been examined, namely, the gas requirements, serum and red cell requirements, frequency of medium replacement, and a comparison of static and agitated cultures made. the most important factors affecting growth in vitro seem to be the oxygen tension, red cell concentration, the frequency with which old medium is replaced ...1979120767
[gonadostatin and gonadocrinin, polypeptides of ovarian origin with hypophysiotropic activity].crude acetic acid extract of rat ovaries pretreated with pregnant mare serum (pmsg) contains native peptides with two types of separable biological activities: one, molecular weight greater than 10,000 dalton inhibits the secretion of both lh and fsh as stimulated by luteinizing hormone releasing factor (lrf) in a pituitary monolayer culture system and is referred to as gonadostatin; the other, less than 3,500 dalton, stimulates the secretion of gonadotropins and is designated as gonadocrinin. t ...1979121254
preparative management of the equine patient with an abdominal crisis. 1979121901
immunological evidence of beta-lactoglobulins in human colostrum and milk.over thirty specimens of breast milk and colostrum were examined by the double diffusion method in agarose gel using antibovine beta-lactoglobulin antisera. cross reactions were obtained showing the presence of beta-lactoglobulins in human milk and colostrum; the strength of these reactions was comparable with those already observed with porcine and equine mammary secretions. identity reactions were obtained between human and sow's milk against anti-bovine beta-lactoglobulin antisera. results ar ...1979121967
improvement of the equine infectious anemia agar gel immunodiffusion test. 1979122030
activity of adenosine deaminase and purine nucleoside phosphorylase in erythrocytes and lymphocytes of man, horse and cattle.1. activities of ada and pnp were measured in erythrocytes and lymphocytes of man, horse and cattle. 2. in bovine hemolysates both enzyme activities are low when compared with activities in human hemolysates. in horse hemolysates both enzyme activities are virtually absent. 3. enzyme activities are consistently lower (about 50%) in intact lymphocytes than in sonicated lymphocytes. this finding suggests that the uptake of nucleosides is rate-limiting for both enzymes in intact lymphocytes. 4. the ...1978122570
[peripheral leukocytes of melanoma-bearing and melanoma-free grey horses of various ages]. 1979122775
clindamycin. a trojan horse?clindamycin is a widely used antibiotic with a spectrum that includes gram-positive bacteria, with the exception of enterococci, and gram-negative anaerobes. toxicities include clindamycin colitis, a pseudomembranous colitis that can be fatal. the colitis usually is related to dosage and duration of therapy, but can occur after ingestion of only several capsules. once diarrhea develops in a patient taking clindamycin, the drug should be stopped and diagnostic measures to define colitis initiated ...1975123444
schistosomus reflexus in an equine fetus. 1975123668
critical tests of suspension, paste, and pellet formulations of cambendazole in the horse.three formulations of cambendazole were evaluated for anthelmintic activity by critical testing method in 21 horses. cambendazole suspension was administered via stomach tube at the dose rate of 10 mg/kg to 3 horses and at the dose rate of 20 mg/kg to 3 horses. cambendazole paste was given intraorally to 8 horses, and the pellet formulation was fed to 7 horses at the dose rate of 20 mg/kg. anthelmintic activity of cambendazole was similar for all dose rates and formulations. gasterophilus intest ...1975123710
skin conditions in horses. 1975124396
symposium on back problems in the horse. (2) the diagnosis of diseases of the horse's back.a description of some of the clinical features of low back pain in the horse has been given and a number of methods for assisting diagnosis considered. as well as a complete clinical examination both at rest and during exercise, a useful diagnostic aid in some chronic cases was the injection of local anaesthetic into the interspinous spaces. a laboratory examination, including haematological and biochemical profiles, was undertaken in all cases. the serum enzymes got and cpk were particularly va ...1975124654
traumatic hyphema and iridocyclitis in the horse.traumatic iridocyclitis and hyphema in the horse usually follow blunt blows to the orbit and eye. the condition is characterized by miosis, ocular hypotony, ciliary flush, swelling of the iris, and hemorrhage with excessive fibrin in the anterior chamber which permits from 2 to 6 weeks. vigorous treatment with mydriatics, topical and systemic corticosteroids is recommended. possible complications include anterior and posterior synechiae, cataracts, and fibropupillary membranes.1975124815
initiation of precocious sexual maturation in the immature rat treated with dehydroepiandrosterone.administration of dehydroepiandrosterone (dha) to immature female rats on day 27 for 3 days resulted in an increase in uterine weight within 6 h of injection and a surge of fsh, lh and prolactin occurred on day 30 resulting in premature ovulation. increase in ovarian weight and vaginal patency also occurred on day 30. ovulations occurred at various times on day 30 and some as late as day 33 and these could be synchronized by the administration of pregnant mare serum gonadotropin (pmsg). the gona ...1975125650
the treatment of horses with chronic back pain by resecting the summits of the impinging dorsal spinous processes.this paper describes the surgical treatment of 14 horses exhibiting chronic back pain attributed to impaction and overriding of the dorsal spines in the thoracolumbar region. the technique involved the resection of the summits of one or more dorsal spines following midline division of the supraspinous ligament. all the horses returned to full work after a convalescent period of at least four months, and 12 of them showed considerable improvement in their form and performance. a post-surgical com ...1975125651
dehydroepiandrosterone-induced polycystic ovaries and acyclicity in the rat.administration of dehydroepiandrosterone (dha) to the immature female rat resulted in one precocious ovulation followed by ovulatory failure. the animals exhibited either "constant-estrus" or "constant-diestrus" vaginal smears, and the ovaries were either polycystic or contained corpus luteum-like structures. serum follicle-stimulating hormone (fsh) and luteinizing hormone (lh) levels showed an ovulatory type surge on day 30, at the time of the precocious ovulation. thereafter, serum fsh levels ...1975125663
leucocyte myosin and its location in the cell.the intracellular location of the binding site of antibody against purified myosin prepared from equine leucocytes was investigated in neutrophils and lymphocytes by electron microscopy using peroxidase-labelled antibody method. the myosin extracted from equine leucocytes could bind skeletal muscle f-actin and the formed complex showed the biophysical and biochemical properties and electron microscopic appearance of actomyosin. on immunodiffusion, the leucocyte myosin formed a single precipitin ...1975126083
radiographic examination of the equine back case. 1975126323
cell-mediated immunity to cell wall antigens of staphylococcus aureus in vitro. ii. the production of chemotactic factor by guinea pig peritoneal exudate cells.peritoneal exudate cells from sensitized guinea pigs were incubated in culture with staphylococcal cell walls. filtered supernatant fluids from the cultures were chemotactic for horse leukocytes. maximum chemotactic activity was observed in supernatant fluids of cultures incubated 1.5 hours. activity disappeared after 4.5 and 18 hours additional incubation.1975126573
[meiogenic function of follicular fluid. gonadotropins and labeling with na2 35so4 in follicular cells and ovocytes in immature mice].the injection in immature mouse of pregnant mare serum gonadotrophin (pmsg) and human chorionic gonadotrophin (hcg) with na2 35so4 in a strict equilibrium allowed us to show the concentration of 35s in the follicular fluid (meiogenic function) and around maturating oocytes (meiosis). this result allow new cytological progress in the field of vertebrates oogenesis.1975126755
[experimental foreign serum pancreatitis. histologic and histochemical findings in the exocrine pancreas of the mouse after repeated intraperitoneal injections of serum of other species (author's transl)].adult white mice were continually treated by intraperitoneal injections of normal serum of various species (neat, horse, man, rabbit, mouse) for 3 hours up to 16 days. control animals received injections of physiological saline under the same conditions. in the mouse pancreas, the repeated intraperitoneal injections of foreign serum conformably resulted in an interstitial edema, a first granulocytic and histiocytic, later on markedly lymphoplasmactyic interstitial inflammation with single dystro ...1975127488
atpase activity and filament formation of partially purified myosin from leucocytes.myosin was isolated from leucocytes in horse arterial blood by the same procedures used for the isolation of myosin from skeletal muscle. the ca2+-, edta-, and mg2+-atpase [ec 3.6.1.3] activities of the protein was 0.148, 0.147, and 0.001 mumoles/min/mg, respectively, in 0.5 m kcl at ph 7.0 and 25 degrees. the ca2+-atpase activity decreased with decrease in the ionic strength. no difference was found between leucocyte myosin and skeletal myosin in the ph profiles of ca2+- and edta-atpases. the r ...1975127790
erythrocyte enzyme activities and glutathione levels of the horse, cat, dog and man. 1975128434
follicular growth: the basic event in the mouse and human ovary.follicular growth is described as a continuum. it goes on at all times, at all ages, uninterrupted by pregnancy or other periods of non-ovulation. a distinction is made between the continuum at the beginning of follicular growth and events concerning the cyclicity at the end of follicular growth, i.e. ovulation. follicles grow sequentially. also large follicles continue to grow until they become atretic or ovulate. no evidence for a pool of large follicles held in reserve could be found. examina ...1975128630
the fine structure of the glycocalyx of equine spermatozoa: a high-resolution cytochemical study.equine spermatozoa were obtained from ejaculates of young stallions. the seminal plasma was removed and the sperm pellets washed three times with 0-15 m-nacl solution before final centrifugation at 4500 g for 15 min. the pellets were fixed in a mixture of 2-5% glutaraldehyde in 0-1 m-cacodylate buffer, ph 7-4, with 0-5% alcian blue and post-fixed in 1% osmium tetroxide with 1% lanthanum nitrate; other samples were treated with ruthenium red. all samples were dehydrated in ascending concentration ...1975128631
embryo transfer in pigs: a method for introducing genetic material into primary specific-pathogen-free herds.fifty-five embryos were transferred from 4 duroc donor sows into 4 crossbred recipient gilts. the embryo survival rate was 66%; 53% of eggs ovulated by donors were represented by live pigs at term. three of the litters were obtained by hysterectomy of the recipients and introduced into a primary specific-pathogen-free herd, thereby saving the donor sows for subsequent use. embryo transfer provided a method for exploiting the genetic potential of superior females.1976129018
dehydroepiandrosterone in the fetal gonads of the horse. 1976130484
pharmacological experiments as a basis for the administration of digoxin in the horse.it is shown that the concentration of ouabain necessary for 50 per cent inhibition of the na+k activated membrane atpase of red cells is similar in man and horse. this is taken to indicate that the two species have similar sensitivity towards cardiac glycosides in general. in five adult healthy horses plasma digoxin concentration was measured with a radioimmunoassay technique after a single intravenous injection of 1 mg/100 kg body weight digoxin. the half time of elimination was 23 h and the ap ...1976130670
fiber types and size in equine skeletal muscle.frozen sections of equine musculus semitendinosus were examined for myosin adenosine triphosphatase (atpase) and reduced nicotinamide adenine dinucleotide-tetrazolium reductase (nadh-tr), using standard histochemical procedures, and the proportions of the various fiber types and average fiber sectional size were determined. with atpase staining, approximately 70% of the fibers were classified as alpha fibers (atpase positive), and 30%, as beta fibers (atpase negative). in addition, 2 populations ...1976130814
assembly of the sarcoplasmic reticulum. biosynthesis of the adenosine triphosphatase in rat skeletal muscle cell culture.temporal patterns of biosynthesis of the ca2+ + mg2+-dependent adenosine triphosphatase of sarcomplasmic reticulum were obtained from studies with primary cultures of rat skeletal muscle cells. rates of synthesis at various stages of differentiation were estimated from the incorporation of tritium-labeled leucine into the atpase. cells were solubilized with detergent, and newly synthesized atpase was isolated from cells by antibody precipitation in the presence of carrier atpase. radioactivity i ...1976131798
the effect of induced hepatic microsomal amidopyrine demethylase activity on the susceptibility of the liver of the calf and horse to carbon disulphide. 1976131811
[cecal motricity and ceco-iliac transit in the horse].electrical spiking activity of the caecum is increased eight-fold and the rate of passage of digesta is increased four-fold when the volume of caecal contents is doubled. the role of caecal motility in regulating the volume of caecal contents is discussed.1975132258
role of adenosine deaminase in lymphocyte proliferation.activity of adenosine deaminase (ada), an enzyme known to be deficient in some patients with severe combined immunodeficiency, increased three-fold within a 24-hour exposure of human peripheral blood lymphocytes to phytohaemagglutinin (pha) in culture. this increase took place before the onset of dna synthesis. increased levels of ada activity were also observed in lymphocytes incubated with pokeweed mitogen (pwm) for 60 hr. dna synthesis induced by pha, pwm or mixed lymphocyte cultures (mlc) wa ...1976133008
[neurophysins of mammals: evolution and biological signification].neurohypophysial hormone-neurophysin complexes have been prepared from posterior pituitary glands of artiodactyla (ox, sheep, pig), perissodactyla (horse) and cetacea (whale), by fractionated salt precipitation. the components have been separated by molecular sieving in 0.2 m acetic acid and neurophysins have been purified by ion-exchange chromatography on deae-sephadex a-50. two types of neurophysins, msel-neurophysins and vldv-neurophysins, can be distinguished according to the amino acid resi ...1976134792
circulating factors controlling cell proliferation.dipolid human fibroblast-rich tissues contain a macromolecule with a molecular weight between 30,000 and 50,000 daltons which will inhibit the proliferation of fibroblasts in the g1 phase of the cell cycle (i.e., inhibit both 3h-thymidine uptake as well as the normal increase in cell number). the inhibitor is destroyed by trypsin but not by ribonuclease or deoxyribonuclease, and it is thermolabile. it has an acid iep. it is not cytotoxic, and its inhibitory activity appears to be completely reve ...1976135264
[therapy with the help of a horse - attempt at a situational analysis (author's transl)].with december 31, 1974 being the target day, the kuratorium für therapeutisches reiten carried out a questionnaire survey amongst its members. of these questionnaires, 89, predominantly completed by physical therapists, were usuable. as a result of this study it was possible for the first time to collect a number of facts relevant to hippotherapy and therapeutic riding based on a total of 154 415 registered equistrian therapies. amongst other things, the survey provided informative details regar ...1975135313
spontaneous and pha-induced rosetting of human blood, tonsil lymphocytes and mlc blasts with sheep, human and horse erythrocytes.compared to peripheral blood lymphocytes the ability of human tonsil t cells and mlc blasts to bind sheep, human and horse erythrocytes was found to be increased. tonsil and mlc t cells were able to bind sheep red blood cells without any cold incubation, i.e. they were 'early' rosettes, and higher percentage of human and horse erythrocyte rosettes were formed by these cells. low doses of phytohaemagglutinin increased the proportion of rosettes between peripheral blood, tonsil, mlc cells and huma ...1976136324
[radiographic elements of the diagnosis of severe sprain. exposure in forced equine varus]. 1975136689
lungworm infection (dictyocaulus arnfieldi) of horses and donkeys.dictyocaulus arnfieldi (cobbold 1884) infects the respiratory tract of horses, donkeys, mules, hinnies and zebra. a review of the literature has been given by round (1972). in the western hemisphere it is popularly believed that donkeys are the natural host and that horses become infected by association with infected donkeys. there is scant documentary evidence for this and, in the soviet union, patent infections may reach 70 per cent without mention of donkey association (koulikov 1935, borovko ...1976136786
assembly of the sarcoplasmic reticulum. biosynthesis of calsequestrin in rat skeletal muscle cell cultures.temporal patterns of biosynthesis of the sarcoplasmic reticulum protein, calsequestrin, were analyzed and compared with rates of atpase synthesis in primary cultures of rat skeletal muscle cells. rates of synthesis were measured by the incorporation of radioactive leucine into the isolated proteins. cells at various stages of differentiation were incubated for 2 h with tritium-labeled leucine and extracted with detergent. the extracts were incubated with antibodies specific against calsequestrin ...1976137241
acupuncture: clinical trials in the horse.a discussion of the merits of acupuncture therapy and a review of experiments that support the neurogenic mediation of acupuncture are presented. case reports are included as examples of the therapeutic value of acupuncture in treatment of intestinal and musculoskeletal disorders in the horse. statistical importance cannot be credited to the clinical experiences reported here because of the small number of cases. hopefully, the clinical success reported will encourage continued investigation of ...1977137886
animal disease agents transmitted by horse flies and deer flies (diptera: tabanidae). 1976137982
treatment of lungworm infestation in horses. 1976138414
studies of urticaria and acute serum sickness with the c1q precipitin test.the c1q precipitin test was performed in serum samples from five groups of patients: (1) 20 patients with acomplementemic systemic lupus erythematosus glomerulonephritis (sle), (2) 2 patients with serum sickness due to the administration of horse serum, (3) 2 patients with serum sickness preceding hepatitis b, (4) 50 patients with chronic urticaria, and (5) 30 normal controls. positive c1q precipitin tests were found in all patients with sle and the four cases of serum sickness. positive tests c ...1977139855
[the mode of action of antifungal agents. effect of horse erythrocyte membranes on amphotericin b].the tris-hcl solution of the heptaen antibiotic, amphotericin b, gives a very important circular dichroism exhibiting a disymetric couplet. the absorption is affected by a blue shift of the maxima. the addition of erythrocyte membranes reduces the couplet and increases the small negative peaks to positive values at 422 and 399 nm. as a time function the evolution of spectra is hyperbol like. such transformations are more important than those obtained with molecular cholesterol. these striking mo ...1976139999
proteases stimulate proliferation of human fibroblasts.incubation of primary human fibroblasts in serum-free medium with small concentrations of thrombin, trypsin or plasmin resulted in manyfold increase in total dna synthesis and in the number of 3h-thymidine labelled cells. rise in the frequency of mitoses indicates that the proteases stimulated also cell division. because proteases induced only a fraction of cells to proliferate increase in the total number of cells remained moderate. calf, horse and rabbit serum inhibited the growth stimulating ...1977140878
[gastrophilus infection in horses and the measures for its control]. 1977142341
the effect of training and detraining on muscle composition in the horse.1. percutaneous needle biopsies were obtained from six limb muscles in six horses before and during a training programme of 10 or 15 weeks designed to involve both aerobic and anaerobic work. in a subsequent detraining period, biopsies were also taken after 5 and 10 weeks. 2. samples were analysed biochemically for enzyme activity of lactic dehydrogenase (ldh), creatine phosphokinase (cpk), aldolase (ald), citrate synthase (cs), aspartate aminotransferase (ast), and alanine aminotransferase (alt ...1977142828
(mg2+ + k+)-dependent inhibition of nak-atpase due to a contaminant in equine muscle atp. 1977143283
the role of hypothalamic serotonin (5ht) before ovulation in immature rats treated with pregnant mare serum (pms). 1977143670
filarial infection of equidae in the tehran area of iran.microfilariae of three genera, namely elaeophora böhmi, onchocerca cervicalis and parafilaria multipapillosa were recovered from blood samples of equidae in tehran, iran. these microfilarial infections in equidae are reported for the first time from iran.1977143741
comparative pathologic classification of malignant lymphomas. 1977143811
aerobic oxidation of p-hydroquinone by horse radish peroxidase in the presence of a thiol and mncl2.in the presence of mncl2 and a thiol (glutathione, cysteine, 2-nitro-5-thiobenzoic acid) horse radish peroxidase oxidizes p-hydroquinone to p-benzoquinone which in turn immediately adds the thiol present yielding 2-s-substituted p-hydroquinone.1977143826
stimulation of prolactin secretion by estrogens and androgens in pms-hcg treated immature rats. 1977144538
[clinical and experimental studies on male infertility. 2. experimental studies. histochemical demonstration of acid phosphatase activity in the testes of mice and their responce to the drugs for the male infertility (author's transl)]. 1977144632
[breeding site on horse flies (diptera, tabanidae) in the vicinity of izhevsk]. 1977144848
[immunologic investigations of house-dust and house-dust mites. i. schultz-dale test (author's transl)].with dermatophagoides pteronyssinus and house-dust sensitized guinea-pigs showed cross-reactions between house-dust, d. pteronyssinus and d. farinae in the schultz-dale test. euroglyphus maynei gave positive reactions only in animals sensitized with d. pteronyssinus. the stored food mites tyrophagus putrescentiae, chortoglyphus arcuatus and glycyphagus destructor and the rearing medium (horse dander/yeast) did not cause any cross-reactions in any test group.1977145169
heparin: a potent inhibitor of ovarian luteinizing hormone-sensitive adenylate cyclase. 1977145375
giant cell tumor of bone: fine structural localization of acid phosphatase.the fine structural localization of acid phosphatase in the different cells in a benign giant cell tumor of bone has been studied. stromal cells type 1 and 2 (fibroblast-like and macrophage-like, respectively) showed the presence of lead phosphate precipitate following incubation in a gomori-type lead salt medium only in conventional lysosomes. in the multinucleated giant cells, the final product was deposited over lysosome-like organelles, and also over golgi cisternae, vesicles, and vacuoles. ...1977145723
mixed lymphocyte culture responses in combined immunodeficiency of horses.combined immunodeficiency in horses is a genetic disorder in which there is a defect in the production of committed b and t lymphocytes. in this study, peripheral blood mononuclear leukocytes from foals with combined immunodeficiency were examined for their capacity to stimulate and respond in one-way mixed lymphocyte cultures. irradiated cells from combined immunodeficient foals were uniformly capable of stimulating cells from unrelated horses. however, none were able to respond to allogeneic s ...1978146281
inhibition of the mixed lymphocyte proliferative response by phorbol esters.the phorbol diester, 12-o-tetradecanoyl-phorbol-13-acetate, a potent cocarcinogen in mice, blocks the induction of dna synthesis in lymphocytes undergoing the mixed lymphocyte response. at 10(-7) m diester, the induced dna synthesis is inhibited almost completely (99%). this action of the diester affects some early step in the response which is necessary for the triggering of cell replication; on-going dna replication is not significantly affected. phorbol 12,13-diacetate, a less potent analogue ...1978146519
microculture method for mixed lymphocyte cultures in the horse.a miniaturized method for the mixed lymphocyte culture test in the horse is described. the test is performed in either round- or flat-bottom microtitration tissue culture plates. concentrations of responsing and stimulating cells are varied, depening on the experiment. significant discrimination between isogeneic and allogenic mixtures is possible after 120 hours' culture when cells are labeled ([3h]thymidine) for the last 16 to 18 hours of the test.1978147041
a dynein atpase inhibitor isolated from a commercial atp preparation.preparations of atp from equine muscle contained an inhibitor of dynein mg2+-activated atpase. the inhibitory material was separated from the atp by molecular sieve filtration. the several molecular species of dynein extracted from three different axonemal sources were all inhibited; myosin atpase was not. with increasing amounts of inhibitor the inhibition did not go to completion but reached a plateau when the rate had been reduced to 1/5 the uninhibited rate. a plot of 1/[s] against 1/v at se ...1978147108
specific and non-specific skin test reactions with animal dander extract.intradermal skin tests have been performed in atopic patients with allergenic extracts of different animal danders at uniform concentration. a high incidence of positive test was recorded which could not be related to the clinical histories, especially with the allergens of horse, cow and dog dander (nr). better correlation was observed with guinea pig and cat allergens (r). the incidence of nr reactions was highest in the house dust allergy group of patients; with the exception of horse dander ...1977147614
lungworm (dictyocaulus arnfieldi) infection in the horse. 1978148137
atopy to dander from domestic animals.atopy to dander from domestic animals occurs rather frequently in atopic patients, especially danders from the cat, guinea-pig, horse, rabbit, and golden hamster. there is no clear sex difference. the age group in which the phenomenon is found most frequently is from 21 to 30 years. as in hay-fever patients during the season, we found a double-peaked frequency curve for the blood eosinophilia. animal-dander atopy was diagnosed particularly in patients with asthma and/or with atopic eczema. the s ...1978150218
the presence of two (na+ + k+)-atpase inhibitors in equine muscle atp: vanadate nad a dithioerythritol-dependent inhibitor.a potent inhibitor of (na+ + k+)-atpase activity was purified from sigma equine muscle atp by cation- and anion-exchange chromatography. the isolated inhibitor was identified by atomic absorption spectroscopy and proton resonance spectroscopy to be an inorganic vanadate. the isolated vanadate and a solution of v2o5 inhibit sarcolemma (na+ + k+)-atpase with an i50 of 1 micrometer in the presence of 1 mm ethyleneglycol-bis-(beta-aminoethylether)-n,n'-tetraacetic acid (egta), 145 mm nacl, 6mm mgcl2 ...1978150289
[how better to organize therapeutic work]. 1978151373
development of the venous drainage of the equine hypophysis cerebri. 1978151511
cryosurgical treatment of equine cutaneous habronemiasis. 1978151968
granulomatous and neoplastic diseases of the skin of horses.lesions encountered in a clinicopathological study of cutaneous lesions resembling 'swamp cancer' from horses in north queensland included 37 cases of subcutaneous phycomycosis, 5 of which were also infected with habronema sp larvae. in addition 9 cases of primary cutaneous habronemiasis, 58 sarcoids, 12 fibromas, 8 fibrosarcomas, 10 squamous cell carcinomas, 4 haemangiomas, 3 melanomas, 1 papilloma, 1 palpebral adenocarcinoma and 7 cases of simple granulation were diagnosed. subcutaneous phycom ...1978152106
[structure and carbohydrate histochemistry of the major salivary glands of the horse (author's transl)]. 1977152587
[melatonin and gonadoliberin (lh-rh) cells in rat hypothalamus].immature female rats were induced to ovulate by an injection of pregnant mare serum: melatonin, intraperitonealy injected, induce an increase of lh-rh immunoreactive neurons in the hypothalamus. these neurons are localized in septo preoptic, infundibular and premammillary areas.1978153177
[evaluation of the serological reponse of cattle infected naturally and experimentally with venezuelan equine encephalitis virus]. 1979154334
differences in the histochemical properties of skeletal muscles of different breeds of horses and dogs.histochemical profiles of individual muscle fibres were established using myosin adenosine triphosphatase (myosin atpase), succinate dehydrogenase (sdhase), and glycogen phosphorylase (gpase) reactions in three muscles (semitendinosus, diaphragm, and pectoralis transversus) of the horse and dog. the major histochemical difference between fibres lies in their myosin atpase activity; fibres can be subdivided into those with a high and those with a low activity. in horse muscle, all fibres have a h ...1978154495
formation of steroids by the pregnant mare. vi. metabolism of [14c]farnesyl pyrophosphate and [3h]dehydroepiandrosterone injected into the fetus.a mixture of [4,8,12-14c]farnesyl pyrophosphate and [3h]dehydroepiandrosterone was injected into a horse fetus im during laparotomy, after which maternal urine was collected for 6 days. steroid conjugates in the urine were extracted with amberlite xad-2 resin, hydrolyzed, and separated into phenolic and neutral fractions. estrone, 17 alpha-estradiol, equilin [3-hydroxy-1,3,5(10),7-estratetraen-17-one], and 17 alpha-dihydroequilin [1,3,4(10),7-estratetraene-3,17 alpha-diol] were isolated from the ...1978155006
[biopreparation production at orsk biologics factory]. 1979155348
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