| effect of dietary macronutrients on the expression of cholecystokinin, leptin, ghrelin and neuropeptide y in gilthead sea bream (sparus aurata). | endocrine factors released from the central nervous system, gastrointestinal tract, adipose tissue and other peripheral organs mediate the regulation of food intake. although many studies have evaluated the effect of fed-to-starved transition on the expression of appetite-related genes, little is known about how the expression of appetite-regulating peptides is regulated by the macronutrient composition of the diet. the aim of the present study was to examine the effect of diet composition and n ... | 2017 | 27725144 |
| performance of maximum likelihood mixture models to estimate nursery habitat contributions to fish stocks: a case study on sea bream sparus aurata. | mixture models (mm) can be used to describe mixed stocks considering three sets of parameters: the total number of contributing sources, their chemical baseline signatures and their mixing proportions. when all nursery sources have been previously identified and sampled for juvenile fish to produce baseline nursery-signatures, mixing proportions are the only unknown set of parameters to be estimated from the mixed-stock data. otherwise, the number of sources, as well as some/all nursery-signatur ... | 2016 | 27761305 |
| having a blast: searchable transcriptome resources for the gilthead sea bream and the european sea bass. | the gilthead sea bream (sparus aurata) and the european sea bass (dicentrarchus labrax) are the most important aquaculture species in the mediterranean sea and since the last decade it has been seen an exponential increase in their available molecular resources. in order to improve accessibility to transcriptome resources, expressed sequence tags (ests), mrna sequences and raw read sequences were assembled and deposited in blast queryable databases. the publicly available sea bream and sea bass ... | 2016 | 27742405 |
| evaluation of a battery of marine species-based bioassays against raw and treated municipal wastewaters. | the present study evaluates a battery of marine species-based bioassays against chemically characterized municipal wastewater samples (raw and wwtp treated). we estimated dunaliella tertiolecta growth rate inhibition (24-96h ic50 values), artemia franciscana immobilization (24h lc50 values), mussel hemocytes viability and lipid peroxidation enhancement (in terms of neutral red retention assay/nrrt and malondialdehyde/mda content, respectively) in influent- and wwtp effluent-treated species. we f ... | 2017 | 27676080 |
| adipogenic gene expression in gilthead sea bream mesenchymal stem cells from different origin. | during the last decades, adipogenesis has become an emerging field of study in aquaculture due to the relevance of the adipose tissue in many physiological processes and its connection with the endocrine system. in this sense, recent studies have translated into the establishment of preadipocyte culture models from several fish species, sometimes lacking information on the mrna levels of adipogenic genes. thus, the aim of this study was to determine the gene expression profile of gilthead sea br ... | 2016 | 27597840 |
| replacement of marine fish oil with de novo omega-3 oils from transgenic camelina sativa in feeds for gilthead sea bream (sparus aurata l.). | omega-3 (n-3) long-chain polyunsaturated fatty acids (lc-pufa) are essential components of the diet of all vertebrates. the major dietary source of n-3 lc-pufa for humans has been fish and seafood but, paradoxically, farmed fish are also reliant on marine fisheries for fish meal and fish oil (fo), traditionally major ingredients of aquafeeds. currently, the only sustainable alternatives to fo are vegetable oils, which are rich in c18 pufa, but devoid of the eicosapentaenoic (epa) and docosahexae ... | 2016 | 27590240 |
| activity of r(+) limonene on the maximum growth rate of fish spoilage organisms and related effects on shelf-life prolongation of fresh gilthead sea bream fillets. | r(+)limonene (lmn) is the major aromatic compound in essential oils obtained from oranges, grapefruits, and lemons. the improvement of preservation techniques to reduce the growth and activity of spoilage microorganisms in foods is crucial to increase their shelf life and to reduce the losses due to spoilage. the aim of this work is to evaluate the effect of lmn on the shelf life of fish fillets. its effectiveness was preliminarily investigated in vitro against 60 strains of specific spoilage or ... | 2016 | 27552348 |
| transcriptomic changes in relation to early-life events in the gilthead sea bream (sparus aurata). | teleosts are exposed to a broad range of external stimuli, which may be either of acute or chronic nature. the larval phase of certain fish species offer a unique opportunity to study the interactions between genes and environmental factors during early life. the present study investigates the effects of early-life events, applied at different time points of early ontogeny (first feeding, flexion and development of all fins; phase 1) as well as on the subsequent juvenile stage after the applicat ... | 2016 | 27461489 |
| tissue-specific gene expression and fasting regulation of sirtuin family in gilthead sea bream (sparus aurata). | the seven sirtuin (sirt) counterparts of higher vertebrates were identified and molecularly characterized in a farmed fish of the sparidae family, order perciformes. these proteins are nad(+)-dependent deacetylases that couple protein deacetylation with the energy status of the cell via the cellular nad(+)/nadh ratio with a strict conservation of the characteristic catalytic domain surrounded by divergent n- and c- terminal regions. phylogenetic analysis showed three major clades corresponding t ... | 2017 | 27431591 |
| dna damage and differential gene expression associated with physical stress in gilthead seabream (sparus aurata). | fish stress may result in inhibition of reproduction, development and growth. thus, appropriate indices should be developed to accurately define the physiological plasticity of fish, in terms of coping with stress. sea bream individuals were subjected to physical stress (fasting and confinement). dna fragmentation of liver cells was assessed, in addition to gene expression of selected genes and plasma cortisol levels determination. stress response was characterized with significant temporal alte ... | 2016 | 27401265 |
| partitioning the metabolic scope: the importance of anaerobic metabolism and implications for the oxygen- and capacity-limited thermal tolerance (ocltt) hypothesis. | ongoing climate change is predicted to affect the distribution and abundance of aquatic ectotherms owing to increasing constraints on organismal physiology, in particular involving the metabolic scope (ms) available for performance and fitness. the oxygen- and capacity-limited thermal tolerance (ocltt) hypothesis prescribes ms as an overarching benchmark for fitness-related performance and assumes that any anaerobic contribution within the ms is insignificant. the ms is typically derived from re ... | 2016 | 27293766 |
| assessment of freshness and freeze-thawing of sea bream fillets (sparus aurata) by a cytosolic enzyme: lactate dehydrogenase. | the evaluation of freshness and freeze-thawing of fish fillets was carried out by assessment of autolysis of cells using a cytosolic enzyme lactate dehydrogenase. autolysis plays an important role in spoilage of fish and postmortem changes in fish tissue are due to the breakdown of the cellular structures and release of cytoplasmic contents. the outflow of a cytosolic enzyme, lactate dehydrogenase, was studied in sea bream fillets and the sparus aurata fibroblasts (saf-1) cell-line during an 8da ... | 2016 | 27211667 |
| nadph production, a growth marker, is stimulated by maslinic acid in gilthead sea bream by increased nadp-idh and me expression. | nadph plays a central role in reductive biosynthesis of membrane lipids, maintenance of cell integrity, protein synthesis and redox balance maintenance. hence, nadph is involved in the growth and proliferation processes. in addition, it has been shown that changes in nutritional conditions produced changes in nadph levels and growth rate. maslinic acid (ma), a pentacyclic triterpene of natural origin, is able to stimulate nadph production, through regulation of the two oxidative phase dehydrogen ... | 2016 | 27178358 |
| jellyfish stings trigger gill disorders and increased mortality in farmed sparus aurata (linnaeus, 1758) in the mediterranean sea. | jellyfish are of particular concern for marine finfish aquaculture. in recent years repeated mass mortality episodes of farmed fish were caused by blooms of gelatinous cnidarian stingers, as a consequence of a wide range of hemolytic, cytotoxic, and neurotoxic properties of associated cnidocytes venoms. the mauve stinger jellyfish pelagia noctiluca (scyphozoa) has been identified as direct causative agent for several documented fish mortality events both in northern europe and the mediterranean ... | 2016 | 27100175 |
| influence of time, storage temperature and freeze/thaw cycles on the activity of digestive enzymes from gilthead sea bream (sparus aurata). | in this study, we tested the effects of long-term storage (2 years) at -20 °c and short-term storage (several hours) in ice and freeze/thaw cycles on the activities of pancreatic, gastric and intestinal (brush border and cytosolic) digestive enzymes in a teleost fish species. the results revealed a significant lose in activity of pancreatic (trypsin, chymotrypsin, total alkaline proteases and α-amylase) and intestinal cytosolic (leucine-alanine peptidase) enzymes between 140 and 270 days of stor ... | 2016 | 27083435 |
| ocean warming alters cellular metabolism and induces mortality in fish early life stages: a proteomic approach. | climate change has pervasive effects on marine ecosystems, altering biodiversity patterns, abundance and distribution of species, biological interactions, phenology, and organisms' physiology, performance and fitness. fish early life stages have narrow thermal windows and are thus more vulnerable to further changes in water temperature. the aim of this study was to address the sensitivity and underlying molecular changes of larvae of a key fisheries species, the sea bream sparus aurata, towards ... | 2016 | 27062348 |
| dietary lipid and carbohydrate interactions: implications on lipid and glucose absorption, transport in gilthead sea bream (sparus aurata) juveniles. | a digestibility trial was performed with gilthead sea bream juveniles (ibw = 72 g) fed four diets differing in lipid source (fish oil, fo; or a blend of vegetable oil, vo) and starch content (0 %, ch-; or 20 %, ch+) to evaluate the potential interactive effects between carbohydrates and vo on the processes involved in digestion, absorption and transport of lipids and glucose. in fish fed vo diets a decrease in lipid digestibility and in cholesterol (c), high density lipoprotein(hdl)-c and low de ... | 2016 | 27023202 |
| fast real time pcr for control of intra-species recycling in aquaculture feed, focused to the most relevant fish species farmed in europe. | recent regulations in animal feed composition prohibit intra-species recycling, the recycling of one given animal species to the same species, in order to avoid potential safety risks to human and animal health. these regulations have generated the need of their control in aquaculture by effective and specific analytical techniques. to date, most studies of species identification and detection in feedstuffs are focused on land species, but few studies are focused on species composition in fish f ... | 2016 | 26988512 |
| determination of sulfadiazine, trimethoprim, and n(4) -acetyl-sulfadiazine in fish muscle plus skin by liquid chromatography-mass spectrometry. withdrawal-time calculation after in-feed administration in gilthead sea bream (sparus aurata l.) fed two different diets. | this study presents a depletion study for sulfadiazine and trimethoprim in muscle plus skin of gilthead sea bream (sparus aurata l.). n(4) -acetyl-sulfadiazine, the main metabolite of sulfadiazine (sdz), was also examined. the fish were held in seawater at a temperature of 24-26 °c. sdz and trimethoprim (tmp) were administered orally with medicated feed for five consecutive days at daily doses of 25 mg sdz and 5 mg tmp per kg of fish body weight per day. two different diets, fish oil- and plant ... | 2016 | 26987772 |
| cytotoxicity and alterations at transcriptional level caused by metals on fish erythrocytes in vitro. | the in vitro use of fish erythrocytes to test the toxicity of aquatic pollutants could be a valuable alternative to fish bioassays but has received little attention. in this study, erythrocytes from marine gilthead sea bream (sparus aurata l.) and european sea bass (dicentrarchus labrax l.) specimens were exposed for 24 h to cd, hg, pb and as and the resulting cytotoxicity was evaluated. exposure to metals produced a dose-dependent reduction in the viability, and mercury showed the highest toxic ... | 2016 | 26976014 |
| molecular endocrine changes of gh/igf1 axis in gilthead sea bream (sparus aurata l.) exposed to different environmental salinities during larvae to post-larvae stages. | the influence of acclimation of the euryhaline gilthead sea bream (sparus aurata) larvae/post-larvae to brackish water on growth, energetic contents, and mrna levels of selected hormones and growth-regulating hypothalamic neurohormones was assessed. specimens from 49 days post-hatching were acclimated during 28 days to two different environmental salinities: 38 and 20 psu (as brackish water). both groups were then transferred to 38 psu and acclimated for an additional week. early juveniles were ... | 2016 | 26947706 |
| comparison of the transcriptional responses of skeletal muscle and bone to a flooding dose of leucine in the gilthead sea bream (sparus aurata). | skeletal muscle, cartilage and bone must function in a co-ordinated fashion during locomotion and growth. in the present study on the gilthead sea bream (sparus aurata) we tested the hypothesis that muscle and bone differ in their responsiveness to stimuli eliciting fast growth, providing a potential mechanism for generating the skeletal deformities observed in aquaculture. to investigate transcription regulation in skeletal muscle and bone we stimulated protein synthesis using a flooding dose o ... | 2016 | 26921774 |
| characterization data of gilthead sea bream (sparus aurata) igf-i receptors (igf-ira/rb). | in this data article we describe the coding sequence of two igf-ir paralogues (igf-ira and igf-irb) obtained from gilthead sea bream embryos. the putative protein architecture (domains and other important motifs) was determined and, amino acid sequences alignment and phylogenetic analysis of both receptors together with igf-ir orthologues from different vertebrates was performed. additionally, a semi-quantitative conventional pcr was done to analyze the mrna expression of both receptors in diffe ... | 2016 | 26904713 |
| potential use of high levels of vegetal proteins in diets for market-sized gilthead sea bream (sparus aurata). | the effect of partial or total dietary substitution of fishmeal (fm) by vegetal protein sources on growth and feed efficiency was carried out in on-growing gilthead sea bream (mean initial weight 131 g). the control diet (fm 100) contained fm as the primary protein source, while in diets fm 25 and fm 0 the fm protein was replaced at 75% and 100%, respectively, by a vegetable protein mixture consisting of wheat gluten, soybean meal, rapeseed meal and crystalline amino acids. diets fm 25 and fm 0 ... | 2016 | 26880503 |
| unraveling the tissue-specific gene signatures of gilthead sea bream (sparus aurata l.) after hyper- and hypo-osmotic challenges. | a custom microarray was used for the transcriptomic profiling of liver, gills and hypothalamus in response to hypo- (38‰ → 5‰) or hyper- (38‰ → 55‰) osmotic challenges (7 days after salinity transfer) in gilthead sea bream (sparus aurata) juveniles. the total number of differentially expressed genes was 777. among them, 341 and 310 were differentially expressed in liver after hypo- and hyper-osmotic challenges, respectively. the magnitude of changes was lower in gills and hypothalamus with aroun ... | 2016 | 26828928 |
| lysine and leucine deficiencies affect myocytes development and igf signaling in gilthead sea bream (sparus aurata). | optimizing aquaculture production requires better knowledge of growth regulation and improvement in diet formulation. a great effort has been made to replace fish meal for plant protein sources in aquafeeds, making necessary the supplementation of such diets with crystalline amino acids (aa) to cover the nutritional requirements of each species. lysine and leucine are limiting essential aa in fish, and it has been demonstrated that supplementation with them improves growth in different species. ... | 2016 | 26808650 |
| morphological characterization of the oral cavity of the gilthead seabream (sparus aurata) with emphasis on the teeth-age adaptation. | gilthead sea bream with different age groups that collected from seawater fisheries, ismailia governorate, egypt, were examined by gross anatomy and scanning electron microscopy to assess the morphological characteristics of the oral cavity. teeth patterns showed that the gilthead sea bream is adapted to the feeding pattern according to age development, as it modified from spiny form teeth in young fishes to obelisk-like teeth and flat dome-like teeth in growing fishes, with differentiation of t ... | 2016 | 26791335 |
| effect of short chain fructooligosaccharides (scfos) on immunological status and gut microbiota of gilthead sea bream (sparus aurata) reared at two temperatures. | the effects of dietary short chain fructooligosaccharides (scfos) incorporation on hematology, fish immune status, gut microbiota composition, digestive enzymes activities, and gut morphology, was evaluated in gilthead sea bream (sparus aurata) juveniles reared at 18 °c and 25 °c. for that purpose, fish with 32 g were fed diets including 0, 0.1, 0.25 and 0.5% scfos during 8 weeks. overall, scfos had only minor effects on gilthead sea bream immune status. lymphocytes decreased in fish fed the 0.1 ... | 2016 | 26721230 |
| sedative effect of 2-phenoxyethanol and essential oil of lippia alba on stress response in gilthead sea bream (sparus aurata). | the anesthetic efficacy of the essential oil of lippia alba (eola) in sparus aurata was evaluated by induction and recovery times of anesthesia. after, specimens were exposed to anesthetics low concentrations for 4h, under nonstress or stress conditions. range 100-300 μl l(-1) eola induced anesthesia. plasmatic cortisol, glucose, lactate, and osmolality enhanced after eola exposure in the undisturbed (uf) and stressed fish (sf). lower corticotropin-releasing hormone binding-protein expression oc ... | 2015 | 26679791 |
| effects of sustained exercise on gh-igfs axis in gilthead sea bream (sparus aurata). | the endocrine system regulates growth mainly through the growth hormone (gh)/insulin-like growth factors (igfs) axis and, although exercise promotes growth, little is known about its modulation of these factors. the aim of this work was to characterize the effects of 5 wk of moderate sustained swimming on the gh-igfs axis in gilthead sea bream fingerlings. plasma igf-i/gh ratio and tissue gene expression of total igf-i and three splice variants, igf-ii, three igf binding proteins, two gh recepto ... | 2016 | 26661095 |
| igf-i and igf-ii effects on local igf system and signaling pathways in gilthead sea bream (sparus aurata) cultured myocytes. | the insulin-like growth factors (igfs) have a fundamental role in a vast range of functions acting through a tyrosine-kinase receptor (igf-ir). igfs in muscle can affect the expression of components of the local igf system, myogenic regulatory factors (mrfs), proliferating (proliferating cell nuclear antigen, pcna) or differentiating molecules (myosin heavy chain, mhc) and, lead to the activation of different signaling pathways. the response of all these genes to igfs incubation at two different ... | 2016 | 26602376 |
| vessel noise pollution as a human threat to fish: assessment of the stress response in gilthead sea bream (sparus aurata, linnaeus 1758). | this study examined the effects of boat noise pollution on the stress indices of gilthead sea bream (sparus aurata, linnaeus 1758). to assess the stress response in these fish, biometric values and plasma parameters such as acth, cortisol, glucose, lactate, haematocrit, hsp70, total protein, cholesterol, triglycerides and osmolarity were analysed. after acclimatization of the animals, the experiment was carried out in a tank fitted with underwater speakers where the fish were exposed to sound tr ... | 2016 | 26581747 |
| insulin-like growth factor 1 (igf-1) regulates prolactin, growth hormone, and igf-1 receptor expression in the pituitary gland of the gilthead sea bream sparus aurata. | the role of insulin-like growth factor 1 (igf-1) on regulation of growth hormone (gh) and prolactin (prl) as well as the possible involvement of igf-1 receptor subtype a (igf-1ra) mrna was assessed in juvenile specimens of sparus aurata. igf-1ra was successfully cloned, and active receptor domains were localized in its mrna precursor. also, phylogenetic analysis of the protein sequence indicated a closer proximity to igf-1ra isoform found in zebrafish and other teleosts, than to the isoform igf- ... | 2016 | 26486515 |
| supplementation with imuno-2865® in gilthead sea bream (sparus aurata linnaeus, 1758): effects on hematological and antioxidant parameters. | the aim of this study was to evaluate the effects of imuno-2865(®) on hematological and antioxidative parameters in sea bream. total of 640 sea bream were fed with diets containing 0 (group 1), 1 (group 2), 10 (group 3) and 25 (group 4) g of imuno-2865(®) kg(-1) feed during 90 days. samples were taken each month and three months after the supplementation. a significant heterophils increase was observed in group 4 compared to group 1 after two months, and an increase in monocytes number was obser ... | 2015 | 26455665 |
| dietary glutamine supplementation effects on amino acid metabolism, intestinal nutrient absorption capacity and antioxidant response of gilthead sea bream (sparus aurata) juveniles. | a study was undertaken to evaluate dietary glutamine supplementation effects on gilthead sea bream performance, intestinal nutrient absorption capacity, hepatic and intestinal glutamine metabolism and oxidative status. for that purpose gilthead sea bream juveniles (mean weight 13.0g) were fed four isolipidic (18% lipid) and isonitrogenous (43% protein) diets supplemented with 0, 0.5, 1 and 2% glutamine for 6weeks. fish performance, body composition and intestinal nutrient absorption capacity wer ... | 2016 | 26424608 |
| energy metabolism of hyperthyroid gilthead sea bream sparus aurata l. | thyroid hormones, in particular 3,5,3'-triiodothyronine or t3, are involved in multiple physiological processes in mammals such as protein, fat and carbohydrate metabolism. however, the metabolic actions of t3 in fish are still not fully elucidated. we therefore tested the effects of t3 on sparus aurata energy metabolism and osmoregulatory system, a hyperthyroid-induced model that was chosen. fish were implanted with coconut oil depots (containing 0, 2.5, 5.0 and 10.0μg t3/g body weight) and sam ... | 2016 | 26419695 |
| growth-promoting effects of sustained swimming in fingerlings of gilthead sea bream (sparus aurata l.). | fish growth is strongly influenced by environmental and nutritional factors and changing culture conditions can help optimize it. the importance of early-life experience on the muscle phenotype later in life is well known. here, we study the effects of 5 weeks of moderate and sustained swimming activity (5 bl s(-1)) in gilthead sea bream during early development. we analysed growth and body indexes, plasma igf-i and gh levels, feed conversion, composition [proximate and isotopic ((15)n/(13)c)] a ... | 2015 | 26391594 |
| xenobiotic-contaminated diets affect hepatic lipid metabolism: implications for liver steatosis in sparus aurata juveniles. | the metabolic effects induced by feed contaminated with a lower or a higher concentration of -nonylpnenol (np), 4-tert-octylphenol (t-op) or bisphenol a (bpa), three environmental endocrine disruptors, were assessed in juvenile sea bream liver. histological analysis demonstrated that all these three xenobiotics induced hepatic lipid accumulation and steatosis. these findings prompted analysis of the expression of the major molecules involved in lipid metabolism: peroxisome proliferator activated ... | 2015 | 26382854 |
| impact of fishmeal replacement in diets for gilthead sea bream (sparus aurata) on the gastrointestinal microbiota determined by pyrosequencing the 16s rrna gene. | recent studies have demonstrated the impact of diet on microbiota composition, but the essential need for the optimization of production rates and costs forces farms and aquaculture production to carry out continuous dietary tests. in order to understand the effect of total fishmeal replacement by vegetable-based feed in the sea bream (sparus aurata), the microbial composition of the stomach, foregut, midgut and hindgut was analysed using high-throughput 16s rdna sequencing, also considering par ... | 2015 | 26317431 |
| identification, phylogeny, and function of fabp2 paralogs in two non-model teleost fish species. | intestinal fatty-acid-binding protein (ifabp or fabp2) is a cytosolic transporter of long-chain fatty acids, which is mainly expressed in cells of intestinal tissue. fatty acids in teleosts are an important source of energy for growth, reproduction, and swimming and a main ingredient in the yolk sac of embryos and larvae. the fabp2 paralogs, fabp2a and fabp2b, were identified for 26 teleost fish species including the paralogs for the two non-model teleost fish species, namely the gilthead sea br ... | 2015 | 26272429 |
| transient up- and down-regulation of expression of myosin light chain 2 and myostatin mrna mark the changes from stratified hyperplasia to muscle fiber hypertrophy in larvae of gilthead sea bream (sparus aurata l.). | hyperplasia and hypertrophy are the two mechanisms by which muscle develops and grows. we study these two mechanisms, during the early development of white muscle in sparus aurata, by means of histology and the expression of structural and regulatory genes. a clear stage of stratified hyperplasia was identified early in the development of gilthead sea bream but ceased by 35 dph when hypertrophy took over. mosaic recruitment of new white fibers began as soon as 60 dph. the genes mlc2a and mlc2b w ... | 2016 | 26246399 |
| labeling and label free shotgun proteomics approaches to characterize muscle tissue from farmed and wild gilthead sea bream (sparus aurata). | the proteome characterization of fish muscle tissues, together with the relative expression of each individual protein, provides knowledge on the biochemical response of the organisms and allows to assess the effect of different types of feeding, growth site and nutritional quality of the investigated species. this type of study is usually performed by gel-based proteomics approaches, however shotgun proteomics can serve as well, reducing analysis time and improving sample high-throughput. in th ... | 2016 | 26233254 |
| exposure of sea bream (sparus aurata) to toxic concentrations of benzo[a]pyrene: possible human health effect. | polycyclic aromatic hydrocarbons (pahs) can accumulate in the food chain, due to their lipophilic properties. fish can accumulate contaminants including pahs and frequent consumption of such contaminated fish can pose risk to human health. the aim of this study was to clarify if acute exposure of sea bream (sparus aurata, a fish species of great economic importance in the atlantic and mediterranean areas) to a pah, benzo[a]pyrene (b[a]p), at a dose that can induce cyp1a and pathological changes ... | 2015 | 26232038 |
| skin mucus proteome of gilthead sea bream: a non-invasive method to screen for welfare indicators. | in teleosts, the skin mucus is the first physical barrier against physical and chemical attacks. it contains components related to metabolism, environmental influences and nutritional status. here, we study mucus and composition based on a proteome map of soluble epidermal mucus proteins obtained by 2d-electrophoresis in gilthead sea bream, sparus aurata. over 1300 spots were recorded and the 100 most abundant were further analysed by lc-ms/ms and identified by database retrieval; we also establ ... | 2015 | 26134830 |
| effects of short-chain fructooligosaccharides (scfos) and rearing temperature on growth performance and hepatic intermediary metabolism in gilthead sea bream (sparus aurata) juveniles. | the effect of dietary short-chain fructooligosaccharides (scfos) incorporation on growth, feed utilization, body composition, plasmatic metabolites and liver activity of key enzymes of lipogenic and amino acid catabolic pathways was evaluated in gilthead sea bream reared at 18 and 25 °c. four practical diets containing plant ingredients and fish meal (50:50) as protein sources and supplemented with 0, 0.1, 0.25 and 0.5 % scfos were fed to triplicate groups of fish for 8 weeks. growth performance ... | 2015 | 26100557 |
| identification of isobaric lyso-phosphatidylcholines in lipid extracts of gilthead sea bream (sparus aurata) fillets by hydrophilic interaction liquid chromatography coupled to high-resolution fourier-transform mass spectrometry. | the numerous and varied biological roles of phosphatidylcholines (pc) and lysophosphatidylcholines (lpc) have fueled a great demand for technologies that enable rapid, in-depth structural examination of these lipids in foodstuffs. here, we describe the capabilities of a newly configured combination of high-efficiency liquid chromatography and high-resolution/accuracy fourier-transform mass spectrometry with electrospray ionization (lc-esi-ftms), designed for lipidomics applications that require ... | 2015 | 25935670 |
| characterization of the ifn pathway in the teleost fish gonad against vertically transmitted viral nervous necrosis virus. | one of the most powerful innate immune responses against viruses is mediated by type i ifn. in teleost fish, it is known that virus infection triggers the expression of ifn and many ifn-stimulated genes, but the viral rna sensors and mediators leading to ifn production are scarcely known. thus, we have searched for the presence of these genes in gilt-head sea bream (sparus aurata) and european sea bass (dicentrarchus labrax), and evaluated their expression after infection with viral nervous necr ... | 2015 | 25918238 |
| characterisation and expression analysis of cathepsins and ubiquitin-proteasome genes in gilthead sea bream (sparus aurata) skeletal muscle. | the proteolytic enzymes involved in normal protein turnover in fish muscle are also responsible for post-mortem softening of the flesh and are therefore potential determinants of product quality. the main enzyme systems involved are calpains, cathepsins, and the ubiquitin-proteasome (ubp). in this study on sparus aurata (sa), the coding sequences of cathepsins (sactsb and sactsdb) and ubp family members (san3 and saub) were cloned from fast skeletal muscle, and their expression patterns were exa ... | 2015 | 25880457 |
| the toxicological impacts of some heavy metals on carbonic anhydrase from gilthead sea bream (sparus aurata) gills. | it is known that heavy metals have toxic effects on fish. insufficient measures are a serious problem in our country and around the world. this problem can threaten human health in areas where it is common for people to obtain nutrition from local bodies of water. in this study, the toxicological impacts of some heavy metals were investigated on carbonic anhydrase activity in gilthead gills. carbonic anhydrase (ca) was purified from gilthead sea bream (sparus aurata) gills with a specific activi ... | 2015 | 25769102 |
| draft genome sequences of vibrio renopiscarius strains cect 8603t and cect 8604, two marine gammaproteobacteria isolated from cultured gilthead sea bream (sparus aurata). | vibrio renopiscarius dcr 1-4-2(t) (cect 8603(t)) and dcr 1-4-12 (cect 8604) were isolated from healthy gilthead sea bream (sparus aurata) from mediterranean fish farms (castellón, spain). their draft genome sequences (30 and 44 contigs, respectively) have 4.3 mbp and a g+c content of 45.2 mol% and contain almost 3,700 protein-encoding genes. | 2015 | 25767236 |
| the influence of acute handling stress on some blood parameters in cultured sea bream (sparus aurata linnaeus, 1758). | the effect of acute handling stress on haematological profile, blood glucose and lactate (secondary stress markers) in cultured sea bream sparus aurata was evaluated. sixty six sparus aurata were used and equally divided into two groups (a and b). group a was not subjected to stress, group b was subjected to acute handling stress. from each fish, biometric data and blood samples were collected to evaluate haematological profile, blood glucose and lactate. unpaired t-test student was applied to e ... | 2015 | 27800375 |
| intraspecific variation in aerobic and anaerobic locomotion: gilthead sea bream (sparus aurata) and trinidadian guppy (poecilia reticulata) do not exhibit a trade-off between maximum sustained swimming speed and minimum cost of transport. | intraspecific variation and trade-off in aerobic and anaerobic traits remain poorly understood in aquatic locomotion. using gilthead sea bream (sparus aurata) and trinidadian guppy (poecilia reticulata), both axial swimmers, this study tested four hypotheses: (1) gait transition from steady to unsteady (i.e., burst-assisted) swimming is associated with anaerobic metabolism evidenced as excess post exercise oxygen consumption (epoc); (2) variation in swimming performance (critical swimming speed; ... | 2015 | 25741285 |
| effects of dietary next enhance®150 on growth performance and expression of immune and intestinal integrity related genes in gilthead sea bream (sparus aurata l.). | gilthead sea bream juveniles were fed different doses (0, 50, 100, 200, 300 ppm) of next enhance®150 (ne) for 9 weeks. feed gain ratio (fgr) was improved by a 10% with all the doses, but feed intake decreased in a dose dependent manner. the optimum inclusion level to achieve maximum growth was set at 100 ppm. the hepatosomatic index did not vary and only at the highest dose, viscerosomatic and splenosomatic indexes were significantly decreased. no significant changes were found in haematological ... | 2015 | 25681752 |
| vasotocin and isotocin regulate aquaporin 1 function in the sea bream. | aquaporins (aqps) are specific transmembrane water channels with an important function in water homeostasis. in terrestrial vertebrates, aqp2 function is regulated by vasopressin (avp) to accomplish key functions in osmoregulation. the endocrine control of aquaporin function in teleosts remains little studied. therefore, in this study we investigated the regulatory role of vasotocin (avtr) and isotocin (itr) receptors in aqp1 paralog gene function in the teleost gilthead sea bream (sparus aurata ... | 2015 | 25573823 |
| spray-dried plasma promotes growth, modulates the activity of antioxidant defenses, and enhances the immune status of gilthead sea bream (sparus aurata) fingerlings. | terrestrial animal byproduct meals, including nonruminant blood meal and blood products, represent the largest and largely untapped safe source of animal protein available within the international market for the aquafeed industry. spray-dried blood and spray-dried plasma (sdp) proteins have long been recognized as high-quality feed ingredients for farmed animals. in this study, we evaluated the inclusion of sdp from porcine blood (sdpp) in growing diets for gilthead sea bream. three isonitrogeno ... | 2015 | 25568376 |
| pthrp regulates water absorption and aquaporin expression in the intestine of the marine sea bream (sparus aurata, l.). | water ingestion by drinking is fundamental for ion homeostasis in marine fish. however, the fluid ingested requires processing to allow net water absorption in the intestine. the formation of luminal carbonate aggregates impacts on calcium homeostasis and requires epithelial hco3(-) secretion to enable water absorption. in light of its endocrine importance in calcium handling and the indication of involvement in hco3(-) secretion the present study was designed to expose the role of the parathyro ... | 2015 | 25562629 |
| avt and it regulate ion transport across the opercular epithelium of killifish (fundulus heteroclitus) and gilthead sea bream (sparus aurata). | the regulatory role of arginine vasotocin (avt) and isotocin (it) in cl(-) secretion was investigated with the short circuit current (isc) technique in opercular epithelia of killifish (fundulus heteroclitus) and gilthead sea bream (sparus aurata). sea bream operculum showed ~4-fold lower number of na/k-atpase immunoreactive cells and ~12-fold lower secretory current than the killifish. in sea bream opercular membranes, the basolateral addition of avt (10(-6) m) significantly stimulated cl(-) se ... | 2015 | 25536332 |
| cortisol modulates vasotocinergic and isotocinergic pathways in the gilthead sea bream. | in the present study, we assessed the responses of the vasotocinergic and isotocinergic systems to chronic stress induced by cortisol administration in the gilthead sea bream (sparus aurata). pituitary and plasma arginine vasotocin (avt) and isotocin (it) levels, as well as hypothalamic pro-vasotocin (pro-vt) and pro-isotocin (pro-it) mrna expression levels, were analysed. in addition, the mrna levels of three receptors, avtr type v1a2, avtr type v2 and itr, were analysed in several target organ ... | 2015 | 25524977 |
| acclimation to different environmental salinities induces molecular endocrine changes in the gh/igf-i axis of juvenile gilthead sea bream (sparus aurata l.). | to assess the role of the gh/igf-i axis in osmotic acclimation of the gilthead seabream sparus aurata, juvenile specimens were acclimated to four environmental salinities: hyposmotic (5 ‰), isosmotic (12 ‰) and hyperosmotic (40 and 55 ‰). the full-length cdnas for both pituitary adenylate cyclase-activating peptide (pacap) and prepro-somatostatin-i (pss-i), the precursor for mature somatostatin-i (ss-i), were cloned. hypothalamic pacap and pss-i, hypophyseal growth hormone (gh) and prolactin (pr ... | 2015 | 25395251 |
| impact of three commercial feed formulations on farmed gilthead sea bream (sparus aurata, l.) metabolism as inferred from liver and blood serum proteomics. | the zootechnical performance of three different commercial feeds and their impact on liver and serum proteins of gilthead sea bream (sparus aurata, l.) were assessed in a 12 week feeding trial. the three feeds, named a, b, and c, were subjected to lipid and protein characterization by gas chromatography (gc) and liquid chromatography-tandem mass spectrometry (lc-ms/ms), respectively. | 2014 | 25342931 |
| second generation genetic linkage map for the gilthead sea bream sparus aurata l. | an updated second linkage map was constructed for the gilthead sea bream, sparus aurata l., a fish species of great economic importance for the mediterranean aquaculture industry. in contrast to the first linkage map which mainly consisted of genomic microsatellites (ssrs), the new linkage map is highly enriched with ssrs found in expressed sequence tags (est-ssrs), which greatly facilitates comparative mapping with other teleosts. the new map consists of 321 genetic markers in 27 linkage groups ... | 2014 | 25307204 |
| diets labelled with (13)c-starch and (15)n-protein reveal daily rhythms of nutrient use in gilthead sea bream (sparus aurata). | all functions in animals rely on daily rhythms, and mealtime can act as a rhythm-marker of nutrients assimilation and use. the effects of meal timing and food composition on carbohydrate use and protein retention of gilthead sea bream were studied. three groups were fed twice a day (10am and at 5pm) for two months with two alternating diets: a commercial diet (cd) and a high-carbohydrate, low-protein diet (ed). the ed/cd group received the ed diet in the morning and the cd diet in the afternoon, ... | 2015 | 25263129 |
| liver transcriptome analysis in gilthead sea bream upon exposure to low temperature. | water temperature greatly influences the physiology and behaviour of teleost fish as other aquatic organisms. while fish are able to cope with seasonal temperature variations, thermal excursions outside their normal thermal range might exceed their ability to respond leading to severe diseases and death.profound differences exist in thermal tolerance across fish species living in the same geographical areas, promoting for investigating the molecular mechanisms involved in susceptibility and resi ... | 2014 | 25194679 |
| microbiological spoilage and investigation of volatile profile during storage of sea bream fillets under various conditions. | volatile organic compound (voc) profile was determined during storage of sea bream (sparus aurata) fillets under air and modified atmosphere packaging (map - co2/o2/n2: 60/10/30) at 0, 5 and 15°c. microbiological, tvb-n (total volatile base nitrogen) and sensory changes were also monitored. shelf-life of sea bream fillets stored under air was 14, 5 and 2days (d) at 0, 5 and 15°c respectively, while under map was 18, 8, and 2d at 0, 5 and 15°c respectively. at the end of shelf life, the total mic ... | 2014 | 25150673 |
| acute stress response in gilthead sea bream (sparus aurata l.) is time-of-day dependent: physiological and oxidative stress indicators. | since fish show daily rhythms in most physiological functions, it should not be surprising that stressors may have different effects depending on the timing of exposure. in this study, we investigated the influence of time of day on the stress responses, at both physiological and cellular levels, in gilthead sea bream (sparus aurata l.) submitted to air exposure for 30 s and then returned to their tank. one hour after air exposure, blood, hypothalamus and liver samples were taken. six fish per e ... | 2014 | 25102424 |
| different stressors induce differential responses of the crh-stress system in the gilthead sea bream (sparus aurata). | the hypothalamus-pituitary-interrenal (hpi) axis, involved in the regulation of the neuroendocrine stress responses, presents important players such as corticotropin-releasing hormone (crh, generally considered as the initiator of this pathway) and crh-binding protein (crh-bp, considered as an antagonist of crh function). crh and crh-bp full-length cdna sequences were obtained from sparus aurata by screening a brain cdna library, and their phylogenetic analysis as well as their roles during acut ... | 2014 | 25088183 |
| dietary differences are reflected on the gut prokaryotic community structure of wild and commercially reared sea bream (sparus aurata). | we compared the gut prokaryotic communities in wild, organically-, and conventionally reared sea bream (sparus aurata) individuals. gut microbial communities were identified using tag pyrosequencing of the 16s rrna genes. there were distinct prokaryotic communities in the three different fish nutritional treatments, with the bacteria dominating over the archaea. most of the bacteria belonged to the proteobacteria, firmicutes, actinobacteria, and bacteroidetes. the number of bacterial operational ... | 2014 | 25066034 |
| isolation of mast cells from the peritoneal exudate of the teleost fish gilthead sea bream (sparus aurata l.). | inflammation is the first response of animals to infection or tissue damage. sparus aurata (perciformes) was the first fish species shown to possess histamine-containing mast cells at mucosal tissues. we report a separation protocol for obtaining highly enriched (over 95% purity) preparations of fish mast cells in high numbers (5-20 million mast cells per fish). the peritoneal exudate of s. aurata is composed of lymphocytes, acidophilic granulocytes, macrophages and mast cells. we separated the ... | 2014 | 25047357 |
| alkylphenolic contaminants in the diet: sparus aurata juveniles hepatic response. | a wide range of endocrine disrupter chemicals can mimic steroid hormones causing adverse health effects. nonylphenol (np) and t-octhylphenol (t-op) are man-made alkylphenolic environmental contaminants possessing controversial endocrine disruption properties. this study has investigated the effects of np and t-op enriched diets on hepatic tissue and biotransformation activities in the liver. to this aim, sea bream juveniles were fed with commercial diet enriched with three different doses of np ... | 2014 | 24975542 |
| wide-gene expression analysis of lipid-relevant genes in nutritionally challenged gilthead sea bream (sparus aurata). | disturbances of lipid metabolism are a major problem in livestock fish and the present study analysed the different tissue expression patterns and regulations of 40 lipid-relevant genes in gilthead sea bream. nineteen sequences, including fatty acid elongases (4), phospholipases (7), acylglycerol lipases (8) and lipase-maturating enzymes (1), were new for gilthead sea bream (genbank, jx975700-jx975718jx975700jx975701jx975702jx975703jx975704jx975705jx975706jx975707jx975708jx975709jx975710jx975711 ... | 2014 | 24946022 |
| urotensin ii inhibits arginine vasotocin and stimulates isotocin release from nerve endings in the pituitary of gilthead sea bream (sparus aurata). | the aim of this in vitro study was to determine whether arginine vasotocin (avt) and isotocin (it) release from nerve endings is affected by urotensin ii (uii) in gilthead sea bream pituitary. primary cultures of pituitary cells were exposed to 10(-12), 10(-10), and 10(-8) m uii for 6, 24, and 48 hr. avt and it contents were determined in the culture media by high performance liquid chromatography (hplc). uii at all doses decreased avt release after 6, 24, and 48 hr of incubation. it release was ... | 2014 | 24888583 |
| igf-i and amino acids effects through tor signaling on proliferation and differentiation of gilthead sea bream cultured myocytes. | skeletal muscle growth and development is controlled by nutritional (amino acids, aa) as well as hormonal factors (insulin-like growth factor, igf-i); however, how its interaction modulates muscle mass in fish is not clearly elucidated. the purpose of this study was to analyze the development of gilthead sea bream cultured myocytes to describe the effects of aa and igf-i on proliferating cell nuclear antigen (pcna) and myogenic regulatory factors (mrfs) expression, as well as on the transduction ... | 2014 | 24882593 |
| adipose tissue and liver metabolic responses to different levels of dietary carbohydrates in gilthead sea bream (sparus aurata). | this study analyzes the effects of replacing dietary lipids by carbohydrates and carbohydrates by fiber on gilthead sea bream growth, as well as lipid and glucose metabolism in adipose tissue and liver over the course of a 15-week feeding trial. six different diets were formulated and fish were classified into two experimental groups sharing one diet. in the first group (ls), fish were fed four diets where lipids were reduced (23%-17%) by increasing carbohydrates (12%-28%) and, the second group ... | 2014 | 24875564 |
| prevalence, morphology, and molecular analysis of serrasentis sagittifer (acanthocephala: palaeacanthocephala: rhadinorhynchidae), a parasite of the gilthead sea bream sparus aurata (sparidae). | seventy specimens of the gilthead sea bream sparus aurata of the red sea were collected during the period from march to november 2013; they were dissected and examined for parasitic acanthocephalans. only 40 (57.14%) specimens were found to be naturally infected with serrasentis sagittifer belonging to family rhadinorhynchidae. the infection was recorded in the intestine, pyloric ceca, and the external surfaces of some internal organs of the infected fish. seasonally, the prevalence of infection ... | 2014 | 24828344 |
| genotoxic damage of benzo[a]pyrene in cultured sea bream (sparus aurata l.) hepatocytes: harmful effects of chronic exposure. | the large majority of studies on the genotoxic hazard of pahs polluted water widely applied the ena assay as versatile tool in large number of wild and farmed aquatic species. nuclear abnormalities are commonly considered to be a direct consequence of genotoxic lesions in dna macromolecule, and such evaluation might be helpful in identifying the genotoxic damage induced by the most harmful pahs such as b[a]p. regarding at the fish species subjected to aquaculture, most of the toxicological data ... | 2014 | 24794860 |
| metabolic and transcriptional responses of gilthead sea bream (sparus aurata l.) to environmental stress: new insights in fish mitochondrial phenotyping. | the aim of the current study was to phenotype fish metabolism and the transcriptionally-mediated response of hepatic mitochondria of gilthead sea bream to intermittent and repetitive environmental stressors: (i) changes in water temperature (t-st), (ii) changes in water level and chasing (c-st) and (iii) multiple sensory perception stressors (m-st). gene expression profiling was done using a quantitative pcr array of 60 mitochondria-related genes, selected as markers of transcriptional regulatio ... | 2014 | 24792819 |
| molecular cloning, characterization and functional assessment of the myosin light polypeptide chain 2 (mylz2) promoter of farmed carp, labeo rohita. | we cloned the 5'-flanking region (1.2 kb) of a muscle-specific gene, encoding myosin light chain 2 polypeptide (mylz2) of a farmed carp, labeo rohita (rohu). sequence analysis using transfac-database search identified the consensus cis acting regulatory elements of tata-box and e (canntg)-box, including the monocyte enhancer factor 2 motif, implying that it is likely to be a functional promoter. the proximal promoter (~620 bp) was highly homologous with that of danio rerio (zebrafish) as compare ... | 2014 | 24740361 |
| biometric and metabolic profiles associated to different rearing conditions in offshore farmed gilthead sea bream (sparus aurata l.). | modern multivariate methods are applied to both biometric measurements and nmr metabolic profiling of fillet to discriminate farmed gilthead sea bream reared in different farming conditions. two fish groups having the same average size, from the same farm, were caught in may and october. biometric data demonstrate that condition factor is higher for the leaner fish, sampled in may, while liver somatic index is lower in fish sampled in october. biometric features are related to metabolic changes ... | 2014 | 24634328 |
| transcriptional assessment by microarray analysis and large-scale meta-analysis of the metabolic capacity of cardiac and skeletal muscle tissues to cope with reduced nutrient availability in gilthead sea bream (sparus aurata l.). | the effects of nutrient availability on the transcriptome of cardiac and skeletal muscle tissues were assessed in juvenile gilthead sea bream fed with a standard diet at two feeding levels: (1) full ration size and (2) 70 % satiation followed by a finishing phase at the maintenance ration. microarray analysis evidenced a characteristic transcriptomic profile for each muscle tissue following changes in oxidative capacity (heart > red skeletal muscle > white skeletal muscle). the transcriptome of ... | 2014 | 24626932 |
| implications for chronic toxicity of benzo[a]pyrene in sea bream cultured hepatocytes: cytotoxicity, inflammation, and cancerogenesis. | benzo[a]pyrene (b[a]p) is the most studied dangerous polycyclic aromatic hydrocarbon for its hepatotoxic, carcinogenic, mutagenic, teratogenic, and immunosuppressant effects, which can affect both wild and farmed marine fish through the trophic chain. this study investigated, for the first time, the chronic effects induced in vitro by b[a]p prolonged exposure on gilthead sea bream (sparus aurata l.) hepatocytes, evaluating the cellular and nuclear latent damage. the purpose was to characterize t ... | 2015 | 24610634 |
| interleukin gene expression is strongly modulated at the local level in a fish-parasite model. | the goal of this work was to identify interleukin (il)-related genes in the gilthead sea bream (gsb) (sparus aurata l.) and how they are modulated by the parasite enteromyxum leei, a myxozoan that causes severe enteritis with a strong inflammatory response. a blast-x search of our transcriptomic gsb database (www.nutrigroup-iats.org/seabreamdb) identified 16 new sequences encompassing seven ils (il-7, il-8, il-10, il-12β, il-15, il-18, and il-34), the interleukin enhancer-binding factor 2 (ilf2) ... | 2014 | 24530812 |
| circadian rhythms of gene expression of lipid metabolism in gilthead sea bream liver: synchronisation to light and feeding time. | this research aimed at investigating circadian rhythm expression of key genes involved in lipid metabolism in the liver of a teleost fish (sparus aurata), and their synchronisation to different light-dark (l-d) and feeding cycles. to this end, 90 gilthead sea bream were kept in 12:12 h (light:dark, ld, lights on at zt0) and fed a single daily meal at mid-light (ml = zt6), mid-darkness (md = zt18) and randomly (rd) at a 1.5% body weight ration. a total of 18 tanks were used, six tanks per feeding ... | 2014 | 24517141 |
| multi-residue and multi-class determination of antibiotics in gilthead sea bream (sparus aurata) by ultra high-performance liquid chromatography-tandem mass spectrometry. | this paper describes a method for the determination of 41 antibiotics from seven different classes in gilthead sea bream (sparus aurata) by ultra-high-performance liquid chromatography with tandem mass spectrometry (uplc-ms/ms). sulfonamides, trimethoprim, tetracyclines, macrolides, quinolones, penicillins and chloramphenicol were simultaneously determined. fourteen procedures for sample treatment were tested and an extraction with acetonitrile and ethylenediaminetetra acetic acid was found to b ... | 2014 | 24512256 |
| endocrine regulation of carbonate precipitate formation in marine fish intestine by stanniocalcin and pthrp. | in marine fish, high epithelial bicarbonate secretion by the intestine generates luminal carbonate precipitates of divalent cations that play a key role in water and ion homeostasis. in vitro studies highlight the involvement of the calciotropic hormones pthrp (parathyroid hormone-related protein) and stanniocalcin (stc) in the regulation of epithelial bicarbonate transport. the present study tested the hypothesis that calciotropic hormones have a regulatory role in carbonate precipitate formati ... | 2014 | 24501133 |
| applications of self-organizing maps for ecomorphological investigations through early ontogeny of fish. | we propose a new graphical approach to the analysis of multi-temporal morphological and ecological data concerning the life history of fish, which can typically serves models in ecomorphological investigations because they often undergo significant ontogenetic changes. these changes can be very complex and difficult to describe, so that visualization, abstraction and interpretation of the underlying relationships are often impeded. therefore, classic ecomorphological analyses of covariation betw ... | 2014 | 24466185 |
| in vitro effect of cortisol and urotensin i on arginine vasotocin and isotocin secretion from pituitary cells of gilthead sea bream sparus aurata. | this study aimed at determining whether in vitro secretion of two neuropeptides, arginine vasotocin (avt) and isotocin (it), from pituitary cells of gilthead sea bream sparus aurata was affected by cortisol and urotensin (ui). pituitary cells were exposed to 1·4 × 10(-8) , 1·4 × 10(-7) and 0·4 × 10(-6) m cortisol and 10(-12) , 10(-10) and 10(-8) m ui for 6, 24 and 48 h, respectively. avt and it contents were determined in the culture media by high-performance liquid chromatography (hplc). an i ... | 2014 | 24405471 |
| mu opioid receptor in spermatozoa, eggs and larvae of gilthead sea bream (sparus aurata) and its involvement in stress related to aquaculture. | in aquaculture, unfavourable conditions experienced during early development may have strong downstream effects on the adult phenotype and fitness. sensitivity to stress, leading to disease, reduced growth and mortality, is higher in larvae than in adult fish. in this study, conducted on sea bream (sparus aurata), we evidenced the presence of the mu opioid receptor in gametes and larvae at different developmental stages. moreover, we evaluated the possibility of reducing the effects of artificia ... | 2014 | 24338156 |
| variations in the expression of vasotocin and isotocin receptor genes in the gilthead sea bream sparus aurata during different osmotic challenges. | the dynamic changes in mrna expression levels for vasotocin (avt) and isotocin (it) receptor gene levels were assessed in a time-course response study in immature male specimens of the gilthead sea bream (sparus aurata) submitted to hyper- (55‰ salinity) and hypo-osmotic (5‰ salinity) challenges. two different cdnas for the avt receptor and one for the it receptor (v1a2-type and v2-type avtr, and itr, respectively) were cloned by screening an s. aurata brain cdna library. genes for these recepto ... | 2014 | 24332959 |
| molecular cloning of four glutathione peroxidase (gpx) homologs and expression analysis during stress exposure of the marine teleost sparus aurata. | glutathione peroxidase (gpx; ec 1.11.1.9) is an important family of enzymes that protects organisms from oxidative damage. four full-length gpx cdnas were cloned and characterized by rapid amplification of cdna ends polymerase chain reaction (race-pcr) from the liver of gilthead sea bream (sparus aurata), an economically important species for mediterranean aquaculture. structural and functional annotations were performed for all paralogs, which suggested possible differences in function and subc ... | 2014 | 24269721 |
| modulation of leukocytic populations of gilthead sea bream (sparus aurata) by the intestinal parasite enteromyxum leei (myxozoa: myxosporea). | summary the cellular mucosal and systemic effectors of gilthead sea bream (gsb) (sparus aurata) involved in the acute immune response to the intestinal parasite enteromyxum leei were studied in fish experimentally infected by the anal route. in the intestinal inflammatory infiltrates and in lymphohaematopoietic organs (head kidney and spleen) of parasitized fish, the number of plasma cells, b cells (igm immunoreactive) and mast cells (histamine immunoreactive) were significantly higher, whereas ... | 2014 | 24230459 |
| dha but not epa, enhances sound induced escape behavior and mauthner cells activity in sparus aurata. | dietary omega-3 long chain polyunsaturated fatty acids (n-3 lcpufa), eicosapentaenoic acid (epa) and docosahexaenoic acid (dha), have a marked effect on fish behavior. there is limited information on the mechanisms involved in this effect and its relation to neuron development and functioning. deficiency of n-3 lcpufa reduces fish escape swimming. mauthner cells (m-cell) are neurons responsible for initiating an escape response. the aim was to compare the effect of dietary dha and epa on escape ... | 2014 | 24184412 |
| characterisation and expression of myogenesis regulatory factors during in vitro myoblast development and in vivo fasting in the gilthead sea bream (sparus aurata). | the aim of this study was to characterise a primary cell culture isolated from fast skeletal muscle of the gilthead sea bream. gene expression profiles during culture maturation were compared with those obtained from a fasting-refeeding model which is widely used to modulate myogenesis in vivo. myogenesis is controlled by numerous extracellular signals together with intracellular transcriptional factors whose coordinated expression is critical for the appropriate development of muscle fibres. fu ... | 2014 | 24157945 |
| tissue-specific gene expression and functional regulation of uncoupling protein 2 (ucp2) by hypoxia and nutrient availability in gilthead sea bream (sparus aurata): implications on the physiological significance of ucp1-3 variants. | the aim of this study was to assess in an integrative manner the physiological regulation of uncoupling protein 2 (ucp2) in gilthead sea bream. a contig of 1,325 nucleotides in length with an open reading frame of 307 amino acids was recognized as ucp2 after searches in our transcriptome reference database ( http://www.nutrigroup-iats.org/seabreamdb ). gene expression mapping by quantitative real-time pcr revealed a ubiquitous profile that clearly differs from that of ucp1 and ucp3 variants with ... | 2014 | 24154671 |
| biophysical evaluation of physiological effects of gilthead sea bream (sparus aurata) farming using ftir spectroscopy. | sparus aurata is one of the two most important cultured fish species in the mediterranean region. the present work investigates the effects of culturing in s. aurata liver tissue at the molecular level using fourier transform infrared (ftir) spectroscopy. ftir spectroscopy revealed dramatic differences between the wild and aquacultured fish liver cells, which mainly indicated that the level of glycogen increased in the aquacultured samples and the protein/lipid ratio decreased by 42.29% indicati ... | 2014 | 24128583 |
| in vitro characterization of acid secretion in the gilthead sea bream (sparus aurata) stomach. | the gastric acid secretion of juvenile sparus aurata was characterized in ussing chambers; secretion rates were determined by a ph-stat method at ph5.50 and bioelectrical parameters were measured in current-clamped tissues. the basal secretion equaled to 535±87nmol·cm(-2)·h(-1). serosal carbachol 100μm produced an increase (δjh(+)) of 725±133nmol·cm(-2)·h(-1) from basal secretion, this effect being inhibited by mucosal omeprazole 100μm. basal secretion was also sensitive to the combination of se ... | 2014 | 24126049 |
| a new type of lordosis and vertebral body compression in gilthead sea bream, sparus aurata l.: aetiology, anatomy and consequences for survival. | a new type of vertebral malformation is described, consisting of deformed cartilaginous neural and haemal processes and the compression and fusion of vertebral bodies. the malformation is designated as haemal vertebral compression and fusion (haemal vcf). we studied the aetiology of the malformations and described microanatomical histopathological alterations. the malformations were detected during routine quality control in one of six monitored gilthead sea bream populations. haemal vcf affecte ... | 2014 | 24117787 |
| characterisation and expression of calpain family members in relation to nutritional status, diet composition and flesh texture in gilthead sea bream (sparus aurata). | calpains are non-lysosomal calcium-activated neutral proteases involved in a wide range of cellular processes including muscle proteolysis linked to post-mortem flesh softening. the aims of this study were (a) to characterise several members of the calpain system in gilthead sea bream and (b) to examine their expression in relation to nutritional status and muscle tenderisation. we identified the complete open reading frame of gilthead sea bream calpains1-3, sacapn1, sacapn2, sacapn3, and two pa ... | 2013 | 24086513 |
| evaluation of the in vitro anti-atherogenic properties of lipid fractions of olive pomace, olive pomace enriched fish feed and gilthead sea bream (sparus aurata) fed with olive pomace enriched fish feed. | given the pivotal role of platelet-activating-factor (paf) in atherosclerosis and the cardio-protective role of paf-inhibitors derived from olive pomace, the inclusion of olive pomace in fish feed has been studied for gilthead sea bream (sparus aurata). the aim of the current research was to elucidate the anti-atherogenic properties of specific hplc lipid fractions obtained from olive pomace, olive pomace enriched fish feed and fish fed with the olive pomace enriched fish feed, by evaluating the ... | 2013 | 24084786 |
| vasotocinergic and isotocinergic systems in the gilthead sea bream (sparus aurata): an osmoregulatory story. | to investigate the physiological roles of arginine vasotocin (avt) and isotocin (it) in osmoregulatory process in gilthead sea bream (sparus aurata), a time course study (0, 12h, and 1, 3, 7 and 14 days) has been performed in specimens submitted to hypoosmotic (from 40‰ salinity to 5‰ salinity) or hyperosmotic (from 40‰ salinity to 55‰ salinity) challenges. plasma and liver osmoregulatory and metabolic parameters, as well as avt and it pituitary contents were determined concomitantly with hypoth ... | 2013 | 24021911 |
| avt is involved in the regulation of ion transport in the intestine of the sea bream (sparus aurata). | the intestine of marine fish plays a crucial role in ion homeostasis by selective processing of ingested fluid. although arginine vasotocin (avt) is suggested to play a role in ion regulation in fish, its action in the intestine has not been demonstrated. thus, the present study investigated in vitro the putative role of avt in intestinal ion transport in the sea bream (sparus aurata). a cdna encoding part of an avt receptor was isolated and phylogenetic analysis revealed it clustered with the v ... | 2013 | 23973797 |