| isolation and molecular identification of the etiological agents of streptococcosis in nile tilapia (oreochromis niloticus) cultured in net cages in lake sentani, papua, indonesia. | infections with streptococcus spp. were observed in nile tilapia cultured in net cages in lake sentani, papua, indonesia. clinical signs included exophthalmia, erratic swimming, ascites in abdominal cavity, and external hemorrhages. four types of bacterial colonies (sk, k10, p20, and m12) were isolated from the brain, kidney, and eyes. based on phenotypic and genetic (16s rdna sequencing) characteristics, the isolates were identified as streptococcus iniae (sk), streptococcus agalactiae (k10 and ... | 2014 | 25392797 |
| comparison of genetic characteristics and pathogenicity of lactococcus garvieae isolated from aquatic animals in taiwan. | seventy-six taiwanese bacterial isolates including 74 from diseased, cultured, aquatic animals (54 grey mullet mugil cephalus, 3 basket mullet chelon alatus, 2 tilapia oreochromis niloticus, 1 grouper epinephelus coioides, 2 yellowfin seabream acanthopagrus latus, 1 borneo mullet chelon macrolepis, 1 bullfrog rana catesbeiana, 1 japanese eel anguilla japonica, and 9 giant freshwater prawns macrobrachium rosenbergii), 1 wild-caught seafood species (squid muscle collected from a restaurant) and 1 ... | 2012 | 23209077 |
| pcbs in fish and their cestode parasites in lake victoria. | polychlorinated biphenyls (pcbs) are classified as persistent organic pollutants (pops) regulated by the stockholm convention (2001). although their production and use was stopped almost three decades ago, pcbs are environmental persistent, toxic, and bioaccumulate in biota. we assessed the levels of 7 pcb congeners (iupac nos. 28, 52, 101, 118, 138, 153, and 180) in sediment and fish (oreochromis niloticus, lates niloticus, and rastrineobola argentea) and evaluated the potential of cestode fish ... | 2016 | 27456696 |
| first report of monogenean flatworms from lake tana, ethiopia: gill parasites of the commercially important clarias gariepinus (teleostei: clariidae) and oreochromis niloticus tana (teleostei: cichlidae). | lake tana is the largest lake in ethiopia and the source of the blue nile. the lake harbours unique endemic cyprinid fish species, as well as the commercially important endemic nile tilapia subspecies oreochromis niloticus tana and the north african catfish clarias gariepinus. its endemicity, especially within the labeobarbus radiation, its conservation importance and its economic indispensability attract scientific interest to the lake's ichthyofauna. fish parasites of lake tana, however, are h ... | 2016 | 27457488 |
| probiotic pediococcus acidilactici modulates both localised intestinal- and peripheral-immunity in tilapia (oreochromis niloticus). | the application of probiotics in aquaculture has received concerted research efforts but the localised intestinal immunological response of fish to probiotic bacteria is poorly understood. therefore, a study was conducted to evaluate the probiotic effect of pediococcus acidilactici on nile tilapia (oreochromis niloticus) with specific emphasis on intestinal health and probiotic levels as well as system level responses such as growth performance, feed utilization and haemato-immunological paramet ... | 2013 | 23871840 |
| [identification and pathological observation of a pathogenic plesiomonas shigelloides strain isolated from cultured tilapia (oreochromis niloticus)]. | a mass mortality of tilapia broke out in an aquaculture farm in panyu, guangdong province in may, 2013. affected fish showed blackening of body color, haemorrhageing on surface, scales shedding, fin rotting, and the presence of yellow liver, dark red spleen, enlarged gallbladder and ascitic fluid in the abdominal cavity. the purpose of this research was isolating and identifying the pathogen. | 2015 | 25958688 |
| occurrence of plesiomonas shigelloides in cultured red hybrid tilapia (oreochromis niloticus) from tropical rivers, east coast malaysia. | a total of 182 isolates of plesiomonas shigelloides were identified from 40 healthy red hybrid tilapia, oreochromis niloticus cultured at two important rivers in terengganu, malaysia namely como river and terengganu river from east coast malaysia. p. shigelloides count in digestive tract content (dtc) and muscle (mus) of red hybrid tilapia cultured at terengganu river was 1000-fold higher than como river. antibiotic susceptibility test was also performed on plesiomonas shigelloides isolates. the ... | 2012 | 24191623 |
| eutrophication, ammonia intoxication, and infectious diseases: interdisciplinary factors of mass mortalities in cultured nile tilapia. | the present study was designed to assess the possible causes of the mass mortalities of nile tilapia oreochromis niloticus at el-behera governorate, egypt, in relationship to environmental and microbiotic factors. water samples were collected from fish farms at different locations and from lake edku to analyze water temperature, water ph, salinity, biological oxygen demand, dissolved oxygen, total ammonia nitrogen, and un-ionized ammonia. a number of moribund and freshly dead fish were sampled a ... | 2016 | 27484819 |
| molecular cloning and expression analysis of small ubiquitin-like modifier (sumo) genes from grouper (epinephelus coioides). | small ubiquitin-like modifier (sumo) is a group of proteins binding to lysine residues of target proteins and thereby modifying their stability, activity and subcellular localization. in the present study, two sumo homolog genes (ecsumo1 and ecsumo2) from grouper (epinephelus coioides) were cloned and characterized. the full-length sequence of ecsumo1 was 749 bp in length and contained a predicted open reading frame of 306 bp encoding 101 amino acids with a molecular mass of 11.34 kda. the full- ... | 2016 | 26616235 |
| genetic characterization of a betanodavirus isolated from a clinical disease outbreak in farm-raised tilapia oreochromis niloticus (l.) in thailand. | betanodavirus infection was diagnosed in larvae of farm-raised tilapia oreochromis niloticus (l.), in central thailand. extensive vacuolar degeneration and neuronal necrosis were observed in histological sections with positive immunohistochemical staining for betanodavirus. molecular phylogenetic analysis was performed based on the nucleotide sequences (1333 bases) of the capsid protein gene. the virus strain was highly homologous (93.07-93.88%) and closely related to red-spotted grouper nervous ... | 2015 | 24164433 |
| the morphology and systematics of rhabdochona paski baylis, 1928 (nematoda: rhabdochonidae), a widespread parasite of freshwater fishes in africa. | nematodes of the genus rhabdochona railliet, 1916, identified as r. paski baylis, 1928, were collected from the intestine of hydrocynus forskahlii (cuvier) (characiformes: alestidae), oreochromis niloticus (linnaeus) and tilapia zillii (gervais) (both perciformes: cichlidae) from lake turkana, kenya during 2007-2009. their morphology was studied in detail using light and scanning electron microscopy. paratypes of r. paski and museum specimens of r. congolensis campana-rouget, 1961 from six other ... | 2013 | 23595492 |
| complement factor d homolog involved in the alternative complement pathway of rock bream (oplegnathus fasciatus): molecular and functional characterization and immune responsive mrna expression analysis. | the complement system serves conventional role in the innate defense against common invading pathogens. complement factor d (cfd) is vital to alternative complement pathway activation in cleaving complement factor b. this catalytic reaction forms the alternative c3 convertase that is crucial for complement-mediated pathogenesis. in this study, rock bream (oplegnathus fasciatus) cfd (ofcfd) was characterized and ofcfd mrna expression was investigated. ofcfd encodes 277 amino acids (aa) for a 30-k ... | 2016 | 27311435 |
| [connection of hepcidin genes from two fish species and their expression in pichia pastoris]. | hepcidin are small cationic peptides with antibacterial activity expressed mainly in the liver of living organisms, and they play important roles in the host's immune response against microbial invasion and regulation of iron metabolism. thus, they are considered to be good substitutes for traditional antibiotics. it is a good choice that the antimicrobial peptides are prepared by recombinant dna expression. in the present study, two hepcidin mature peptide cdnas from channel catfish (ictalurus ... | 2015 | 26571689 |
| the study on the candidate probiotic properties of encapsulated yeast, saccharomyces cerevisiae jcm 7255, in nile tilapia (oreochromis niloticus). | saccharomyces cerevisiae jcm 7255 was tested as a probiotic candidate in tilapia after encapsulating and freeze drying. viability and morphology during storage and during transit through simulated gut and bile conditions were determined. growth performance, anti-streptococcal activity and gut mucosal immune parameters were also tested. the viability of encapsulated yeasts was significantly high in simulated gastric and bile conditions and remained high after storage at room temperature for 14 da ... | 2015 | 26412528 |
| screening of multiple hormonal activities in water and sediment from the river nile, egypt, using in vitro bioassay and gonadal histology. | in egypt, until yet no records are available regarding possible multiple hormonal activities in the aquatic systems and especially in the river nile. in this paper, in vitro yeast estrogen screen (yes) and yeast androgen screen (yas) were used to assess (for the first time) the multiple hormonal activities in surface waters and sediments of the river nile. this study aimed to determine whether river nile water can cause changes in gonadal histology of nile tilapia (oreochromis niloticus niloticu ... | 2015 | 25937496 |
| acute aerocystitis in nile tilapia bred in net cages and supplemented with chromium carbochelate and saccharomyces cerevisiae. | oreochromis niloticus bred in net cages were supplemented with cell wall of saccharomyces cerevisiae (sc) (0.3%) or chromium carbochelate (cr) (18 mg/kg of feed) or in association (sc + cr), for 90 days. after this period, acute inflammation was induced in the swim bladder by inoculation of 3 × 10(8) cfu of inactivated streptococcus agalactiae, and another group received 0.65% saline solution (control). twelve, 24, and 48 h after stimulation, the inflammation was evaluated through total and diff ... | 2014 | 24309138 |
| characterization of fatty acid delta-6 desaturase gene in nile tilapia and heterogenous expression in saccharomyces cerevisiae. | fatty acid delta-6 desaturase (fads2)-like gene from nile tilapia (oreochromis niloticus) was characterized and designated as oni-fads2. the oni-fads2 showed the typical structure of microsomal fads2. the presence of oni-fads2 transcripts in unfertilized eggs demonstrated the maternal role of nile tilapia in providing the oni-fads2 transcript in their eggs. in addition, the expression of oni-fads2 was detectable in embryos throughout the hatching stage. real-time reverse transcription-pcr reveal ... | 2013 | 23939229 |
| inflammatory responses of nile tilapia oreochromis niloticus to streptococcus agalactiae: effects of vaccination and yeast diet supplement. | this study evaluated the effects of dietary supplementation with 0.3% saccharomyces cerevisiae yeast cell wall and of vaccination against streptococcus agalactiae on the cellular component of acute inflammation induced in the coelomic cavity of nile tilapia oreochromis niloticus and on survival of the fish after challenge. a total of 84 tilapia of mean (±sd) weight 125.0 ± 1.5 g were distributed among twelve 310 l fiberglass tanks according to a 2 × 2 × 3 factorial design in the following manner ... | 2012 | 22535873 |
| effects of dietary yeast (saccharomyces cerevisia) supplementation in practical diets of tilapia (oreochromis niloticus). | a 51-day feeding trial was carried out to determine the effects of various dietary levels of brewer's yeast, saccharomyces cerevisiae, in the growth performance, body composition and nutrient utilization in nile tilapia, oreochromis niloticus, juveniles. fish (7.6 ± 0.3 g) were stocked into eighteen 1,000-l tanks (100 fish per tank; n = 3) and fed to apparent satiation six isonitrogenous (27% crude protein) and isoenergetic (19 kj/g) diets, formulated to contain different dried yeast levels (0%, ... | 2012 | 26486773 |
| effect of lime juice on vibrio parahaemolyticus and salmonella enterica inactivation during the preparation of the raw fish dish ceviche. | ceviche is a raw fish dish common in peru and other latin american counties. the most characteristic feature of ceviche is the use of lime juice for marinating or "cooking" the raw fish. confirmed cases of cholera in peru, new jersey, and florida have been associated with ceviche. although the effect of organic acids on pathogenic bacteria has been well characterized, few data exist on the effect of these acids in seafood systems. the objective of the study was to evaluate the effects of lime ju ... | 2013 | 23726199 |
| quality properties, fatty acids, and biogenic amines profile of fresh tilapia stored in ice. | this work determines quality properties and fatty acids content of nile tilapia (oreochromis niloticus) stored in ice for 21 d. the quality properties consist of thiobarbituic acid (tba), total volatile basic nitrogen (tvb-n), trimethylamine (tma), and microbiological analysis (total viable count (tvc), total coliform, salmonella and staphylococcus aureus) and determination of biogenic amines content (histamine, cadaverine, putrescine, spermine, spermidine, 2-phenylethylamine, agmatine, tyramine ... | 2013 | 23865452 |
| molecular serotyping, virulence gene profiling and pathogenicity of streptococcus agalactiae isolated from tilapia farms in thailand by multiplex pcr. | this study aimed to biotype streptococcus agalactiae isolated from tilapia farms in thailand based on molecular biotyping methods and to determine the correlation between the serotype and virulence of bacteria. in addition to a biotyping (serotyping) technique based on multiplex pcr of cps genes, in this study, we developed multiplex pcr typing of group b streptococcus (gbs) virulence genes to examine 3 clusters of virulence genes and their correlation to the pathogenicity of s. agalactiae. the ... | 2017 | 28295891 |
| molecular identification and histopathological study of natural streptococcus agalactiae infection in hybrid tilapia (oreochromis niloticus). | the main objective of this study was to emphasize on histopathological examinations and molecular identification of streptococcus agalactiae isolated from natural infections in hybrid tilapia (oreochromis niloticus) in temerloh pahang, malaysia, as well as to determine the susceptibility of the pathogen strains to various currently available antimicrobial agents. | 2017 | 28246454 |
| gapa, a potential vaccine candidate antigen against streptococcus agalactiae in nile tilapia (oreochromis niloticus). | streptococcosis due to the bacterium streptococcus agalactiae (s. agalactiae) has resulted in enormous economic losses in aquaculture worldwide, especially in the tilapia culture industry. previously, there were limited vaccines that could be employed against streptococcosis in tilapia. this study aimed to develop a vaccine candidate using the glyceraldehyde-phosphate dehydrogenase protein (gapa) of s. agalactiae encoded by the gapa gene. tilapia were intraperitoneally injected with pbs, pbs + f ... | 2017 | 28219739 |
| draft genome sequence of an attenuated streptococcus agalactiae strain isolated from the gut of a nile tilapia (oreochromis niloticus). | streptococcus agalactiae is a pathogen that causes severe anthropozoonosis within a broad range of hosts from aquatic animals to mammals, including human beings. here, we describe the draft genome of s. agalactiae hzausc001, a low-virulent strain isolated from the gut of a moribund tilapia (oreochromis niloticus) in china. | 2017 | 28183773 |
| transcriptomic profiling analysis of tilapia (oreochromis niloticus) following streptococcus agalactiae challenge. | innate immune system is the primary defense mechanism against pathogen infection in teleost, which are living in pathogen-rich aquatic environment. it has been long hypothesized that the disease resistance in teleost are strongly correlated to the activities of innate immune genes. tilapia is an important economical fish around the world, especially in china, where the production accounts for nearly half of the global production. recently, s. agalactiae has become one of the most serious bacteri ... | 2017 | 28111359 |
| the effects of dietary kefir and low molecular weight sodium alginate on serum immune parameters, resistance against streptococcus agalactiae and growth performance in nile tilapia (oreochromis niloticus). | the present study evaluates the effects of dietary kefir and low molecular weight sodium alginate (lwmsa) (singular or combined) on non-specific immune response, disease resistance and growth performance of nile tilapia (oreochromis niloticus). fish with average weight of 18.60 ± 0.04 g were supplied and randomly stocked in sixteen glass tanks (150 l) at density of 20 fish per tank. fish were fed experimental diets as follows: 0 g kg(-1) lmwsa (control, diet 1), 10 g kg(-1) lmwsa (diet 2), 40 g ... | 2017 | 28088470 |
| pathological analysis, detection of antigens, fasl expression analysis and leucocytes survival analysis in tilapia (oreochromis niloticus) after infection with green fluorescent protein labeled streptococcus agalactiae. | the pathogenesis of streptococcus agalactiae infection in tilapia has not been fully described. to understand this, we investigated the clinic-pathological features of acute experimental septicemia in tilapia (oreochromis niloticus) after receiving an intra-peritoneal injection with s. agalactiae thn-1901gfp. immunohistochemistry and sections of pathological tissues were used to estimate the level of damage in the head-kidney, liver, spleen and trunk-kidney. the expression of fasl was analyzed b ... | 2017 | 28063953 |
| development of streptococcus agalactiae vaccines for tilapia. | vaccination is a widely accepted and effective method to prevent most pathogenic diseases in aquaculture. various species of tilapia, especially nile tilapia oreochromis niloticus, are farmed worldwide because of their high consumer demand. recently, the tilapia-breeding industry has been hampered by outbreaks of streptococcus agalactiae infection, which cause high mortality and huge economic losses. many researchers have attempted to develop effective s. agalactiae vaccines for tilapia. this re ... | 2016 | 28000606 |
| an overview of vaccination strategies and antigen delivery systems for streptococcus agalactiae vaccines in nile tilapia (oreochromis niloticus). | streptococcus agalactiae is an emerging infectious disease adversely affecting nile tilapia (niloticus oreochromis) production in aquaculture. research carried out in the last decade has focused on developing protective vaccines using different strategies, although no review has been carried out to evaluate the efficacy of these strategies. the purpose of this review is to provide a synopsis of vaccination strategies and antigen delivery systems currently used for s. agalactiae vaccines in tilap ... | 2016 | 27983591 |
| analysis of hematologic alterations, immune responses and metallothionein gene expression in nile tilapia (oreochromis niloticus) exposed to silver nanoparticles. | in this study, nile tilapia (oreochromis niloticus) fingerlings were used as a model to examine acute and chronic toxicity of silver nanoparticles (agnp). expression levels of metallothionein (mt) transcripts in fish exposed to 0, 1 or 100 mg agnp/kg fish were investigated by quantitative real-time rt-pcr. the results showed mt expression levels were significantly decreased 0.3-0.7-fold in the liver and spleen of fish exposed to 1 or 100 mg agnp/kg after 6-48 h. in contrast, during this period, ... | 2016 | 27967301 |
| integrated analysis neurimmirs of tilapia (oreochromis niloticus) involved in immune response to streptococcus agalactiae, a pathogen causing meningoencephalitis in teleosts. | micrornas (mirnas) are a class of noncoding rna molecules and play important roles in a wide spectrum of biological processes, including in immune response. recent years have witnessed considerable amount of research interest in studies on mirna-mediated modulation gene function during neuroinflammation. here, we evaluated streptococcus agalactiae infected tilapia (oreochromis niloticus) brain for the expression profile of mirnas, potential functions and their correlation with genes involved in ... | 2017 | 27956091 |
| the effect of feed-based vaccination on tilapia farm endemic for streptococcosis. | a tilapia farm experiencing endemic streptococcosis was selected to study the effect of vaccination with a feed-based vaccine on naturally ocurring streptococcosis. a total of 9000 red tilapia, oreochromis niloticus × oreochromis mossambicus of 100 ± 20 g were divided into 9 cages. fish of group 1 in cages 1, 2 and 3 were not vaccinated. group 2 in cages 4, 5 and 6 were vaccinated on days 0 and 14 (single booster) while group 3 in cages 7, 8 and 9 were vaccinated on days 0, 14 and 42 (double boo ... | 2017 | 27864157 |
| laboratory-controlled challenges of nile tilapia (oreochromis niloticus) with streptococcus agalactiae: comparisons between immersion, oral, intracoelomic and intramuscular routes of infection. | streptococcus agalactiae, the aetiological agent of streptococcosis in fish, is an important pathogen of cultured and wild fish worldwide. to gain a better understanding of the pathogenesis of streptococcosis in nile tilapia (oreochromis niloticus), and to identify the experimental route of infection that most closely mimics natural disease, fingerlings were challenged with s. agalactiae utilizing different delivery methods. fingerlings were challenged via intracoelomic injection (icinj), intram ... | 2016 | 27817831 |
| feed-based vaccination regime against streptococcosis in red tilapia, oreochromis niloticus x oreochromis mossambicus. | streptococcosis is an important disease of tilapia throughout the world. in malaysia, streptococcosis outbreak was commonly reported during the 3-month period of high water temperature between april and july. this study describes the duration of protection following single and double booster dose regimes against streptococcosis in tilapia using a feed-based vaccine containing formalin-killed streptococcus agalactiae. a total of 510 tilapias of 120 ± 10 g were selected and divided into 3 groups. ... | 2016 | 27608936 |
| complete genome sequence of streptococcus agalactiae strain s25 isolated from peritoneal liquid of nile tilapia. | streptococcus agalactiae (lancefield group b; gbs) is one of the major pathogens in fish production, especially in nile tilapia (oreochromis niloticus). the genomic characteristics of gbs isolated from fish must be more explored. thus, we present here the genome of gbs s25, isolated from nile tilapia from brazil. | 2016 | 27491974 |
| major surfome and secretome profile of streptococcus agalactiae from nile tilapia (oreochromis niloticus): insight into vaccine development. | streptococcus agalactiae is a major piscine pathogen that is responsible for huge economic losses to the aquaculture industry. safe recombinant vaccines, based on a small number of antigenic proteins, are emerging as the most attractive, cost-effective solution against s. agalactiae. the proteins of s. agalactiae exposed to the environment, including surface proteins and secretory proteins, are important targets for the immune system and they are likely to be good vaccine candidates. to obtain a ... | 2016 | 27327442 |
| effects of low molecular weight sodium alginate on growth performance, immunity, and disease resistance of tilapia, oreochromis niloticus. | present study was carried out to evaluate the effects of low molecular weight sodium alginate (lmwsa) as potential prebiotic source on growth performance, innate immunity and disease resistance of tilapia, oreochromis niloticus. three hundred twenty fish were divided into four treatments and fed following diets 0 (t1- control), 10 (t2), 20 (t3) and 30 (t4) g kg(-1) lmwsa for period of 60 days. a completely randomized design with four replications was applied. at the end of experiment, fish in ea ... | 2016 | 27238426 |
| comprehensive identification and profiling of nile tilapia (oreochromis niloticus) micrornas response to streptococcus agalactiae infection through high-throughput sequencing. | micrornas are a kind of small non-coding rnas that participate in various biological processes. deregulated microrna expression is associated with several types of diseases. tilapia (oreochromis niloticus) is an important commercial fish species in china. to identify mirnas and investigate immune-related mirnas of o. niloticus, we applied high-throughput sequencing technology to identify and analyze mirnas from tilapia infected with streptococcus agalactiae at a timescale of 72 h divided into si ... | 2016 | 27050313 |
| molecular and functional characterization of peptidoglycan-recognition protein sc2 (pgrp-sc2) from nile tilapia (oreochromis niloticus) involved in the immune response to streptococcus agalactiae. | pgrp-sc2, the member of pgrp family, plays an important role in regulation of innate immune response. in this paper, a pgrp-sc2 gene of nile tilapia, oreochromis niloticus (designated as on-pgrp-sc2) was cloned and its expression pattern under the infection of streptococcus agalactiae was investigated. sequence analysis showed main structural features required for amidase activity were detected in the deduced amino acid sequence of on-pgrp-sc2. in healthy tilapia, the on-pgrp-sc2 transcripts cou ... | 2016 | 27033804 |
| molecular cloning and expression analysis of igd in nile tilapia (oreochromis niloticus) in response to streptococcus agalactiae stimulus. | igd is considered to be a recently-evolved ig and a puzzling molecule, being previously found in all vertebrate taxa, except for birds. although igd likely plays an important role in vertebrate immune responses, the function of igd in nile tilapia (oreochromis niloticus) is virtually unknown. in the present study, a membrane form of igd (migd) heavy chains were cloned from the gift strain of nile tilapia (designated on-migd). the on-migd heavy chain's cdna is composed of 3347 bp with a 31 bp of ... | 2016 | 27005611 |
| discovery and expression of 3 siglecs-like in oreochromis niloticus neutrophil, and their interaction with group b streptococcal sialylated capsular polysaccharides. | sialic acid - binding immunoglobulin - like lectins (siglecs) are members of the largest superfamily of immune receptors; they recognize sialic acid and are mainly expressed in immune cells. studies on mammals indicate that streptococcus agalactiae (gbs) evade immune reactions by interacting with the host immune cells via the sialic acid of sialylated capsular polysaccharides. however, it is currently unknown if fish-derived gbs can interact with siglecs to evade host immunity. in this study, we ... | 2016 | 26847490 |
| electrotransfer of the tilapia piscidin 3 and tilapia piscidin 4 genes into skeletal muscle enhances the antibacterial and immunomodulatory functions of oreochromis niloticus. | tilapia piscidin 3 (tp3) and tilapia piscidin 4 (tp4) are antimicrobial peptides recently isolated from oreochromis niloticus. we previously showed that synthetic tp3 and tp4 possessed antimicrobial activities. here, we analyzed the bactericidal abilities and immunomodulatory properties of these amps following the electroporation of pcmv-gfp-tp3 or pcmv-gfp-tp4 plasmid into tilapia (o. niloticus) muscle and subsequent infection with vibrio vulnificus or streptococcus agalactiae. prior overexpres ... | 2016 | 26828260 |
| molecular characterization and expression of cd2 in nile tilapia (oreochromis niloticus) in response to streptococcus agalactiae stimulus. | the cluster of differentiation 2 (cd2), functioning as a cell adhesion and costimulatory molecule, plays a crucial role in t-cell activation. in this paper, the cd2 gene of nile tilapia, oreochromis niloticus (designated as on-cd2) was cloned and its expression pattern under the stimulation of streptococcus agalactiae was investigated. sequence analysis showed on-cd2 protein consists of two extracellular ig-like domains, a transmembrane region, and a long proline-rich cytoplasmic tail, which is ... | 2016 | 26804651 |
| serotype distribution and antimicrobial susceptibilities of streptococcus agalactiae isolated from infected cultured tilapia (oreochromis niloticus) in thailand: nine-year perspective. | streptococcus agalactiae (group b streptococcus, gbs) infection remains a major problem associated with high mortality of cultured tilapia worldwide. the present study reports the serotype distribution and antimicrobial susceptibilities of gbs isolated from infected tilapia cultured in thailand. one hundred and forty-four gbs isolates were identified by biochemical, serological and molecular analyses. of these 144 gbs isolates, 126 were serotype ia and 18 were serotype iii. antimicrobial suscept ... | 2016 | 26701807 |
| effects of some dietary crude plant extracts on the growth and gonadal maturity of nile tilapia (oreochromis niloticus) and their resistance to streptococcus agalactiae infection. | a 90-day feeding trial was conducted on the growth performance, feeding efficacy, body indices, various hematological and plasma biochemical parameters, and histopathological examination of the gonads from male and female nile tilapia fingerlings when fed different crude plant extracts from cinnamomum camphora, euphorbia hirta, azadirachta indica, or carica papaya at 2 g kg(-1) compared to a control diet. this was followed by a 14-day challenge to streptococcus agalactiae. all treatments were tr ... | 2016 | 26643907 |
| molecular characterization of galectin-8 from nile tilapia (oreochromis niloticus linn.) and its response to bacterial infection. | galectins belong to the family of galactoside-binding proteins and play a major role in the immune and inflammatory responses of vertebrates and invertebrates. the galectin family is divided into three subtypes based on molecular structure; prototypes, chimera types, and tandem-repeated types. we isolated and characterized the cdna of galectin-8 (ongal-8) in nile tilapia (oreochromis niloticus). ongal-8 consisted of a 966 bp open reading frame (orf) that encoded a 321 amino acid protein (43.47 k ... | 2015 | 26602818 |
| uncaria tomentosa increases growth and immune activity in oreochromis niloticus challenged with streptococcus agalactiae. | cat's claw (uncaria tomentosa) is an amazon herb using in native cultures in peru. in mammals, it has been described several effects of this herb. however, this is the first report of its use on the diet of fish. the aim of this study was to determinate the effect of this plant on the growth and immune activity in oreochromis niloticus. nile tilapia (81.3 ± 4.5 g) were distributed into 5 groups and supplemented with 0 (non-supplement fish), 75, 150, 300, and 450 mg of u. tomentosa.kg(-1) of diet ... | 2015 | 26434713 |
| application of probiotic, prebiotic and synbiotic for the control of streptococcosis in tilapia oreochromis niloticus. | one of the fish diseases that is becoming the main problem in tilapia culture is streptococcosis caused by streptococcus agalactiae. application of probiotic, prebiotic and synbiotic are expected to be an alternative for controlling the disease. the purpose of this study was to examine the effectiveness of the administration of probiotic, prebiotic and synbiotic through artificial feed to control streptococcosis in tilapia. this study consisted of five treatments with three replications, namely ... | 2015 | 26364355 |
| development of a quantitative pcr assay for monitoring streptococcus agalactiae colonization and tissue tropism in experimentally infected tilapia. | streptococcus agalactiae has become one of the most important emerging pathogens in the aquaculture industry and has resulted in large economic losses for tilapia farms in china. in this study, three pairs of specific primers were designed and tested for their specificities and sensitivities in quantitative real-time polymerase chain reactions (qpcrs) after optimization of the annealing temperature. the primer pair igs-s/igs-a, which targets the 16s-23s rrna intergenic spacer region, was finally ... | 2016 | 25858765 |
| cloning and primary immunological study of tgf-β1 and its receptors tβr i /tβr ii in tilapia(oreochromis niloticus). | the transforming growth factor β (tgf-β) superfamily plays critical roles in tumor suppression, cell proliferation and differentiation, tissue morphogenesis, lineage determination, cell migration and apoptosis. recently, tgf-β1, one important member of tgf-β superfamily, is suggested as an immune regulator in the teleost. in this study, we cloned the cdnas of tgf-β1 and its receptors, tβr i and tβr ii (including three isoforms) from tilapia (genbank accession numbers: kp754231- kp754235). a tiss ... | 2015 | 25819083 |
| pharmacokinetics of cefquinome in tilapia (oreochromis niloticus) after a single intramuscular or intraperitoneal administration. | the pharmacokinetics of cefquinome was studied in plasma after a single dose (10 mg/kg) of intramuscular (i.m.) or intraperitoneal (i.p.) administration to tilapia (oreochromis niloticus) in freshwater at 30 °c. ten fish per sampling point were examined after treatment. the data were fitted to two-compartment open models following both routes of administration. the estimates of total body clearance (cl/f), volume of distribution (vd/f), and absorption half-life (t1/2ka ) were 0.049 and 0.037 l/h ... | 2015 | 25707428 |
| molecular and functional characterization of cd59 from nile tilapia (oreochromis niloticus) involved in the immune response to streptococcus agalactiae. | cd59, the major inhibitor of membrane attack complex, plays a crucial role in regulation of complement activation. in this paper, a cd59 gene of nile tilapia, oreochromis niloticus (designated as on-cd59) was cloned and its expression pattern under the stimulation of streptococcus agalactiae was investigated. sequence analysis showed main structural features required for complement-inhibitory activity were detected in the deduced amino acid sequence of on-cd59. in healthy nile tilapia, the on-cd ... | 2015 | 25661843 |
| proteomic analysis of tilapia oreochromis niloticus streptococcus agalactiae strains with different genotypes and serotypes. | nine tilapia oreochromis niloticus group b streptococcus (gbs) strains differing in serotype and genotype were selected and paired. two-dimensional difference gel electrophoresis (2d dige) and matrix-assisted laser-desorption ionization time-of-flight-mass spectrometry (maldi-tof-ms) were used to analyse the protein profiles of the strain pairs. forty-three proteins corresponding to 66 spots were identified, of which 35 proteins were found in the seven selected strain pairs that represented pair ... | 2015 | 25604844 |
| draft genome sequences of streptococcus agalactiae strains isolated from nile tilapia (oreochromis niloticus) farms in thailand. | during 2009-2011, two clinical and one environmental strains of streptococcus agalactiae were isolated from nile tilapia (oreochromis niloticus) farms in thailand. draft genome sequences of two clinical isolates comprise 2,048,343 and 2,105,006 bp, while environmental isolates comprise 2,097,115 bp, having 1,573 to 1,578 coding sequences, respectively. | 2014 | 25502682 |
| molecular characterization and expression of lck in nile tilapia (oreochromis niloticus) in response to streptococcus agalactiae stimulus. | lymphocyte-specific protein tyrosine kinase (lck) plays a critical role in effective signal transductions that are fundamental to t cell differentiation, proliferation, and effector functions. in this paper, the lck gene of nile tilapia, oreochromis niloticus (designated as on-lck), was cloned and its expression pattern under the stimulation of streptococcus agalactiae was investigated. sequence analysis showed important structural characteristics required for t cell receptor (tcr) signal transd ... | 2015 | 25492689 |
| genomic comparison of virulent and non-virulent streptococcus agalactiae in fish. | streptococcus agalactiae infections in fish are predominantly caused by beta-haemolytic strains of clonal complex (cc) 7, notably its namesake sequence type (st) 7, or by non-haemolytic strains of cc552, including the globally distributed st260. in contrast, cc23, including its namesake st23, has been associated with a wide homeothermic and poikilothermic host range, but never with fish. the aim of this study was to determine whether st23 is virulent in fish and to identify genomic markers of fi ... | 2016 | 25399660 |
| molecular characterization and expression of cd2bp2 in nile tilapia (oreochromis niloticus) in response to streptococcus agalactiae stimulus. | cd2bp2 (cd2 cytoplasmic tail binding protein 2), one of several proteins interacting with the cytoplasmic tail of cd2, plays a crucial role in cd2-triggered t cell activation and nuclear splicing. the studies on cd2bp2 have tended to be confined to a few mammals, and little information is available to date regarding fish cd2bp2. in this paper, a cd2bp2 gene (on-cd2bp2) was cloned from nile tilapia, oreochromis niloticus. sequence analysis showed that the full length of on-cd2bp2 cdna was 1429 bp ... | 2014 | 25020258 |
| increasing of temperature induces pathogenicity of streptococcus agalactiae and the up-regulation of inflammatory related genes in infected nile tilapia (oreochromis niloticus). | temperature strongly affects the health of aquatic poikilotherms. in nile tilapia (oreochromis niloticus), elevated water temperatures increase the severity of streptococcosis. here we investigated the effects of temperature on the vulnerability and inflammatory response of nile tilapia to streptococcus agalactiae (group b streptococci; gbs). at 35 and 28 °c, gbs took 4 and 7h, respectively to reach the log-phase and, when incubated with tilapia whole blood, experienced survival rates of 97% and ... | 2014 | 24856132 |
| molecular characterization and virulence gene profiling of pathogenic streptococcus agalactiae populations from tilapia ( oreochromis sp.) farms in thailand. | streptococcus spp. were recovered from diseased tilapia in thailand during 2009-2010 ( n = 33), and were also continually collected from environmental samples (sediment and water) from tilapia farms for 9 months in 2011 ( n = 25). the relative percent recovery of streptococci from environmental samples was 13-67%. all streptococcal isolates were identified as s. agalactiae (group b streptococci [gbs]) by a species-specific polymerase chain reaction. in molecular characterization assays, 4 genoty ... | 2014 | 24842288 |
| characterization and expression analysis of the transferrin gene in nile tilapia (oreochromis niloticus) and its upregulation in response to streptococcus agalactiae infection. | in this study, full-length tilapia transferrin (ontf) isolated from liver cdna of nile tilapia (oreochromis niloticus) was found to have an open reading frame of 2,091-bp encoding 696 amino acid residues. two additional amino acids: gly(369) and gly(370) were observed compared with the reported nile tilapia transferrin protein sequence. pre-mature protein has a predicted molecular weight of 78.2 kda, while mature protein is 73.28 kda in size. comparative sequence analysis with transferrin from o ... | 2014 | 24770882 |
| safety and immunogenicity of an oral dna vaccine encoding sip of streptococcus agalactiae from nile tilapia oreochromis niloticus delivered by live attenuated salmonella typhimurium. | attenuated salmonella typhimurium sl7207 was used as a carrier for a reconstructed dna vaccine against streptococcus agalactiae. a 1.02 kb dna fragment, encoding for a portion of the surface immunogenic protein (sip) of s. agalactiae was inserted into pvax1. the recombinant plasmid pvax1-sip was transfected in epc cells to detect the transient expression by an indirect immunofluorescence assay, together with western blot analysis. the pvax1-sip was transformed by electroporation into sl7207. the ... | 2014 | 24631734 |
| carrier state induced by oxytetracycline therapy against streptococcosis in nile tilapia, oreochromis niloticus (l.). | | 2014 | 24131201 |
| transcriptome profiling and digital gene expression analysis of nile tilapia (oreochromis niloticus) infected by streptococcus agalactiae. | tilapia is an important freshwater aquaculture species worldwide. in recent years, streptococcal diseases have severely threatened development of tilapia aquaculture, while effective prevention and control methods have not yet been established. in order to understand the immunological response of tilapia to infection by streptococcus agalactiae (s. agalactiae), this study employed solexa/illumina rna-seq and digital gene expression (dge) technology to investigate changes in the tilapia transcrip ... | 2013 | 24068429 |
| complete genome sequence of streptococcus agalactiae strain sa20-06, a fish pathogen associated to meningoencephalitis outbreaks. | streptococcus agalactiae (lancefield group b; gbs) is the causative agent of meningoencephalitis in fish, mastitis in cows, and neonatal sepsis in humans. meningoencephalitis is a major health problem for tilapia farming and is responsible for high economic losses worldwide. despite its importance, the genomic characteristics and the main molecular mechanisms involved in virulence of s. agalactiae isolated from fish are still poorly understood. here, we present the genomic features of the 1,820, ... | 2013 | 23991251 |
| chronic streptococcosis in nile tilapia, oreochromis niloticus (l.), caused by streptococcus agalactiae. | | 2014 | 23952820 |
| evaluation of reference genes for quantitative real-time rt-pcr analysis of gene expression in nile tilapia (oreochromis niloticus). | quantitative real-time reverse-transcriptase polymerase chain reaction (rt-qpcr) has been used frequently to study gene expression related to fish immunology. in such studies, a stable reference gene should be selected to correct the expression of the target gene. in this study, seven candidate reference genes (glyceraldehyde-3-phosphate dehydrogenase (gadph), ubiquitin-conjugating enzyme (ubce), 18s ribosomal rna (18s rrna), beta-2-microglobulin (b2m), elongation factor 1 alpha (ef1a), tubulin ... | 2013 | 23792389 |
| transmission of streptococcus agalactiae from a hatchery into a newly established red hybrid tilapia, oreochromis niloticus (l.) × oreochromis mossambicus (peters), farm. | | 2013 | 23347250 |
| major histocompatibility complex class iia and iib genes of nile tilapia oreochromis niloticus: genomic structure, molecular polymorphism and expression patterns. | major histocompatibility complex (mhc) is a large genomic region characterized by extremely high polymorphism, and it plays an important role in the immune response of vertebrates. in the present study, we isolated mhc class ii genes from nile tilapia in order to investigate the immune mechanism in tilapia and develop better strategies for disease prevention. moreover, we cloned the full-length cdna sequences of mhc iia and iib from nile tilapia by the race approach. in addition, the genomic str ... | 2013 | 23261509 |
| characterization of tilapia (oreochromis niloticus) viperin expression, and inhibition of bacterial growth and modulation of immune-related gene expression by electrotransfer of viperin dna into zebrafish muscle. | viperin is an anti-viral protein, induced by viral infection. in this study, we examined whether over-expression of viperin in fish muscle could inhibit bacterial growth. we first obtained the cdna sequence of tilapia viperin, through rt-pcr-mediated cloning and sequencing. the cdna sequence was similar to those of several fish viperins in genbank, and it was predicted to encode the conserved domain of radical s-adenosylmethionine superfamily proteins. phylogenetic analysis revealed that tilapia ... | 2013 | 23237906 |
| five different piscidins from nile tilapia, oreochromis niloticus: analysis of their expressions and biological functions. | piscidins are antimicrobial peptides (amps) that play important roles in helping fish resist pathogenic infections. through comparisons of tilapia est clones, the coding sequences of five piscidin-like amps (named tp1∼5) of nile tilapia, oreochromis niloticus, were determined. the complete piscidin coding sequences of tp1, -2, -3, -4, and -5 were respectively composed of 207, 234, 231, 270, and 195 bases, and each contained a translated region of 68, 77, 76, 89, and 64 amino acids. the tissue-sp ... | 2012 | 23226256 |
| effect of sophora flavescens on non-specific immune response of tilapia (gift oreochromis niloticus) and disease resistance against streptococcus agalactiae. | the paper describes the effect of a diet supplemented with the chinese traditional herbal medicine sophora flavescens on the immunity and disease resistance of an oreochromis niloticus gift strain. experimental diets containing 0.025%, 0.050%, 0.100%, 0.200%, and 0.400% s. flavescens, as well as a control group without s. flavescens were used. we tested the non-specific humoral immune responses (lysozyme, antiprotease, and complement) and cellular immune responses (reactive oxygen species and ni ... | 2013 | 23092731 |
| complete genome sequence of streptococcus agalactiae zq0910, a pathogen causing meningoencephalitis in the gift strain of nile tilapia (oreochromis niloticus). | streptococcus agalactiae (group b streptococcus [gbs]) is a pathogen that causes meningoencephalitis in nile tilapia (oreochromis niloticus). here, we reported the complete genome sequence of s. agalactiae strain zq0910, which was isolated from the gift strain of nile tilapia in guangdong, china. | 2012 | 22933761 |
| genotyping of streptococcus dysgalactiae strains isolated from nile tilapia, oreochromis niloticus (l.). | streptococcus dysgalactiae is an emerging fish pathogen that is responsible for outbreaks of disease on fish farms around the world. recently, this bacterium was associated with an outbreak at a nile tilapia, oreochromis niloticus (l.), farm in brazil. the aim of this study was to evaluate the genetic diversity, best genotyping method and aspects of molecular epidemiology of s. dysgalactiae infections in nile tilapia farms in brazil. twenty-one isolates from four farms located in different brazi ... | 2014 | 23786245 |
| inhibition of mir-92d-3p enhances inflammation responses in genetically improved farmed tilapia (gift, oreochromis niloticus) with streptococcus iniae infection by modulating complement c3. | micrornas (mirnas) are small, non-coding rnas that regulate target gene expression by binding to the 3'-untranslated regions (3'-utrs) of their target mrnas. the mir-92 family is an important mirna family, which was discovered to be related to regulation of tumor proliferation, apoptosis, invasion, and metastasis. inhibition of mir-92d-3p was found previously in head kidney of genetically improved farmed tilapia (gift, oreochromis niloticus) exposed to streptococcus iniae infection. in this stud ... | 2017 | 28235637 |
| effects of exposure to streptococcus iniae on microrna expression in the head kidney of genetically improved farmed tilapia (oreochromis niloticus). | genetically improved farmed tilapia (gift, oreochromis niloticus) are susceptible to infection by streptococcus iniae when maintained in modern intensive culture systems. gift are commercially important fishes that are cultured widely in southern china. the role of micrornas (mirnas) in the regulatory response of gift to s. iniae infection has been underestimated and has not yet been well studied. head kidney has an important immune function in teleost fishes. the main aim of this study was to d ... | 2017 | 28219342 |
| complete genome sequence of streptococcus iniae uel-si1, isolated in diseased nile tilapia (oreochromis niloticus) from northern paraná, southern brazil. | the streptococcus iniae uel-si1 strain was isolated from diseased nile tilapia within the paranapanema river basin, northern paraná, brazil. this is an emerging infectious disease agent of fish from brazil, and sequencing of the complete genome is fundamental to understanding aspects relative to pathogenesis, infection, epidemiology, and immunity. | 2017 | 28082497 |
| effect of n-acetyl cysteine and glycine supplementation on growth performance, glutathione synthesis, anti-oxidative and immune ability of nile tilapia, oreochromis niloticus. | an 8-week feeding trial was conducted to evaluate the effect of n-acetyl cysteine (nac) and glycine supplementation on growth performance, glutathione (gsh) synthesis, anti-oxidative and immune ability of nile tilapia, oreochromis niloticus. four practical diets were formulated, control, control +0.2% nac, control +0.5% glycine, control +0.2% nac +0.5% glycine. each diet was randomly assigned to quadruplicate groups of 30 fish (approximately 9.5 g). the weight gain and specific growth rate were ... | 2016 | 27235905 |
| thermal preference predicts animal personality in nile tilapia oreochromis niloticus. | environmental temperature gradients provide habitat structure in which fish orientate and individual thermal choice may reflect an essential integrated response to the environment. the use of subtle thermal gradients likely impacts upon specific physiological and behavioural processes reflected as a suite of traits described by animal personality. in this study, we examine the relationship between thermal choice, animal personality and the impact of infection upon this interaction. we predicted ... | 2016 | 27219014 |
| trancriptomic profiling revealed the signatures of acute immune response in tilapia (oreochromis niloticus) following streptococcus iniae challenge. | streptococcus iniae is the most significant bacterial disease of tilapia throughout the world, and commonly leads to tremendous economic losses. in contrast to other important fish species, our knowledge about the molecular mechanisms of tilapia in response to bacterial infection is still limited. here, therefore, we utilized rna-seq to first profiling of host responses in tilapia spleen following s. iniae infection at transcriptome level. a total of 223 million reads were obtained and assembled ... | 2015 | 26117728 |
| dietary aloe vera improves plasma lipid profile, antioxidant, and hepatoprotective enzyme activities in gift-tilapia (oreochromis niloticus) after streptococcus iniae challenge. | the current study investigated the effects of dietary aloe vera on plasma lipid profile status, antioxidant, and hepatoprotective enzyme activities of gift-tilapia juveniles under streptococcus iniae challenge. five dietary groups were designed including a control and 100 % aloe powder incorporated into a tilapia feed at 0.5, 1, 2, and 4 %/kg feed, which were administered for 8 weeks. fish fed dietary aloe at 4 %/kg feed significantly reduced in total cholesterol, while triacylglycerol reduced ( ... | 2015 | 26109009 |
| construction of a streptococcus iniae sortase a mutant and evaluation of its potential as an attenuated modified live vaccine in nile tilapia (oreochromis niloticus). | streptococcus iniae is a major gram-positive aquatic pathogen, which causes invasive diseases in cultured fish worldwide. the identification of potential virulence determinants of streptococcal infections will help to understand and control this disease, but only a few have been confirmed in s. iniae. sortase a (srta) is the key enzyme that anchors pre-mature cell wall-attached proteins to peptidoglycan and it can affect the correct positioning of surface proteins, as well as the course of gram- ... | 2014 | 25090938 |
| identification of multiple genes and their expression profiles in four strains of oreochromis spp. in response to streptococcus iniae. | two subtractive complementary dna libraries were constructed from nile tilapia oreochromis niloticus vaccinated with formalin-killed streptococcus iniae cells, and a further two constructed from o. niloticus infected with s. iniae. of the 68 distinct expressed sequence tag (est) contigs and singletons, 45 and 13 est shared high similarities with genes of known and unknown functions, respectively. ten est contigs and singletons had no significant similarity to any sequences. five putative immune- ... | 2013 | 23398064 |
| establishment of a model of streptococcus iniae meningoencephalitis in nile tilapia (oreochromis niloticus). | streptococcus iniae is an invasive pathogen causing meningitis and other lesions in various fish species. furthermore, s. iniae is an emerging zoonotic agent that causes cellulitis in man. the aims of this study were to establish an intraperitoneal infection model for s. iniae in nile tilapia (oreochromis niloticus) and to develop a new histopathological scoring system to reflect the degree and extent of inflammation as well as the presence of necrosis in the brain and eye. intraperitoneal admin ... | 2013 | 23218409 |
| interacting effects of water temperature and dietary protein level on hematological parameters in nile tilapia juveniles, oreochromis niloticus (l.) and mortality under streptococcus iniae infection. | based on central composite rotatable experimental design and response surface method, the interacting effects of temperature (20 °c-34 °c) and dietary protein level (25%-50%) on hematological parameters including red blood cell (rbc), white blood cell (wbc) and hemoglobin (hb) of juvenile oreochromis niloticus were studied under laboratory conditions. the experiment lasted for 7 weeks. after the feeding trial, fish were challenged with streptococcus iniae and mortality was recorded for within 8 ... | 2013 | 23041506 |
| streptococcus iniae outbreaks in brazilian nile tilapia (oreochromis niloticus l:) farms. | this is the first report of outbreaks of streptococcus iniae in nile tilapia farms in south america. seven isolates were identified by biochemical, serological and molecular tests. their 16s rrna gene sequences showed 100% similarity with s. iniae atcc 29178 and two distinct pfge patterns were observed for brazilian isolates. | 2012 | 24031866 |
| influence of moderate hypoxia on vaccine efficacy against vibrio anguillarum in oreochromis niloticus (nile tilapia). | hypoxia is known as a potential immunomodulator in fish. this study therefore assesses the impact of chronic, moderate hypoxia on vaccine efficacy in oreochromis niloticus. serum antibody titer was used as a surrogate marker to detect vaccine efficacy. the fish were acclimatized to either moderate hypoxia (55 ± 5% do) or normoxia (85 ± 5%do) and immunized with formalin inactivated vibrio anguillarum. significantly, a higher antibody titer was found in normoxic fish than in moderate hypoxia. the ... | 2016 | 26915308 |
| transcriptome analysis of the effect of polyunsaturated fatty acids against vibrio vulnificus infection in oreochromis niloticus. | vibrio vulnificus infection causes severe economic losses in oreochromis niloticus aquaculture by inducing pro-inflammatory cytokines, that lead to inflammation and mortality. omega-3 fatty acids, such as docosahexaenoic acid (dha) and eicosapentaenoic acid (epa), have been reported for their anti-inflammatory and antibacterial abilities in murine and zebrafish models. however, the anti-inflammatory and antibacterial functions of dha and epa in commercial aquaculture organisms such as oreochromi ... | 2017 | 28108339 |
| study of the antimicrobial activity of tilapia piscidin 3 (tp3) and tp4 and their effects on immune functions in hybrid tilapia (oreochromis spp.). | to address the growing concern over antibiotic-resistant microbial infections in aquatic animals, we tested several promising alternative agents that have emerged as new drug candidates. specifically, the tilapia piscidins are a group of peptides that possess antimicrobial, wound-healing, and antitumor functions. in this study, we focused on tilapia piscidin 3 (tp3) and tp4, which are peptides derived from oreochromis niloticus, and investigated their inhibition of acute bacterial infections by ... | 2017 | 28085905 |
| assessment of heavy-metal pollution in sediments and tilapia fish species in kafue river of zambia. | we report results from an evaluation of the levels of heavy metals, i.e., copper (cu), cadmium (cd), lead (pb), nickel (ni), manganese (mn), chromium (cr), and iron (fe) in sediment and tilapia fish samples from a wide stretch of the kafue river of zambia. in sediment samples, the highest pb and fe concentrations were recorded at hippo dam, i.e., 36.2 ± 0.1 mg/kg dw and 733 ± 37 mg/kg dw at kafue town, respectively. other notably high metal concentrations in sediment were cr at kafue bridge (42. ... | 2016 | 27401599 |
| oestrogen and insulin-like growth factors during the reproduction and growth of the tilapia oreochromis niloticus and their interactions. | oestrogens and insulin-like growth factors (igfs) play both a central role in the regulation of reproduction and growth and can interact especially in species showing a clear-cut sex-linked growth dimorphism (sgd) like in tilapia. aromatase is essential in ovarian differentiation and oogenesis since it controls oestrogen synthesis. during tilapia sex differentiation, aromatase cyp19a1a expression increases from 9 days post-fertilization (dpf), resulting in high oestradiol level. high temperature ... | 2014 | 25058367 |
| status and future perspectives of vaccines for industrialised fin-fish farming. | fin fish farming is developing from extensive to intensive high industrial scale production. production of fish in high-density growth conditions requires effective vaccines in order to control persistent and emerging diseases. vaccines can also have significant positive impact on the reduced usage of antibiotics. this was demonstrated when vaccines were introduced in norway for atlantic salmon (salmo salar) in the late eighties and early nineties, resulting in a rapid decline of antibiotics con ... | 2013 | 23769873 |
| amounts and compositional analysis of glycosaminoglycans in the tissue of fish. | we isolated gags from the inedible parts; head, skin, internal organs, fins, scales and spine, of atlantic mackerel (scomber scombrus), japanese jack mackerel (trachurus japonicus), pacific bluefin tuna (thunnus orientalis), yellowfin sole (limanda aspera), broadbanded thornyhead (sebastolobus macrochir), golden threadfin bream (nemipterus virgatus), and nile tilapia (oreochromis niloticus). we also investigated deep-sea fish, eelpouts (bothrocara hollandi, lycodes toyamensis, and lycodes nakamu ... | 2013 | 23261779 |
| insulin-like growth factor-3 (igf-3) in male and female gonads of the tilapia: development and regulation of gene expression by growth hormone (gh) and 17alpha-ethinylestradiol (ee2). | recently, in addition to igf-1 and igf-2 the existence of a third form of igf, termed igf-3, limited to fishes, to be present only in the gonads and encoded by a separate gene has been reported. however, no further data have been presented on igf-3. the present study on tilapia (oreochromis niloticus) uses quantitative real-time pcr specific for tilapia igf-1 and igf-3. the organ distribution of igf-3 mrna in adult fish and the early ontogeny of igf-3 in male and female gonads were studied. the ... | 2010 | 20138177 |
| cloning, characterization, and tissue distribution of prolactin receptor in the sea bream (sparus aurata). | the prolactin receptor (prlr) was cloned and its tissue distribution characterized in adults of the protandrous hermaphrodite marine teleost, the sea bream (sparus aurata). an homologous cdna probe for sea bream prlr (sbprlr) was obtained by rt-pcr using gill mrna. this probe was used to screen intestine and kidney cdna libraries from which two overlapping clones (1100 and 2425 bp, respectively) were obtained. these clones had 100% sequence identity in the overlapping region (893 bp) and were us ... | 2001 | 11161768 |
| characterization of genes encoding prolactin and prolactin receptors in starry flounder platichthys stellatus and their expression upon acclimation to freshwater. | this study aims to investigate the genes encoding prolactin (prl) and prolactin receptors (prlr) and their tissue-specific expression in starry flounder platichthys stellatus. starry flounder prl gene consisting of five exons encodes an orf of 212 amino acid residue comprised of a putative signal peptide of 24 amino acids and a mature protein of 188 amino acids. it showed amino acid identities of 73 % with tuna thunnus thynnus, 71 % with black porgy acanthopagrus schlegelii, 69 % with nile tilap ... | 2013 | 22843312 |
| laboratory evaluation of aqueous leaf extract of tephrosia vogelii against larvae of aedes albopictus (diptera: culicidae) and non-target aquatic organisms. | mosquito control using insecticides has been the most successful intervention known to reduce malaria prevalence or incidence. however, vector control is facing a threat due to the emergence of resistance to synthetic insecticides. insecticides of botanical origin may serve as suitable alternative biocontrol techniques in the future. in this research, the leaf aqueous leachate of tephrosia vogelii was evaluated for its toxicity against larvae of the most invasive mosquito worldwide, aedes albopi ... | 2015 | 25771114 |
| cytogenotoxicity screening of source water, wastewater and treated water of drinking water treatment plants using two in vivo test systems: allium cepa root based and nile tilapia erythrocyte based tests. | biological effect directed in vivo tests with model organisms are useful in assessing potential health risks associated with chemical contaminations in surface waters. this study examined the applicability of two in vivo test systems viz. plant, allium cepa root based tests and fish, oreochromis niloticus erythrocyte based tests for screening cytogenotoxic potential of raw source water, water treatment waste (effluents) and treated water of drinking water treatment plants (dwtps) using two dwtps ... | 2017 | 27836175 |
| combination of physico-chemical analysis, allium cepa test system and oreochromis niloticus erythrocyte based comet assay/nuclear abnormalities tests for cyto-genotoxicity assessments of treated effluents discharged from textile industries. | bioassays for cyto-genotoxicity assessments are generally not required in current textile industry effluent discharge management regulations. the present study applied in vivo plant and fish based toxicity tests viz. allium cepa test system and oreochromis niloticus erythrocyte based comet assay and nuclear abnormalities tests in combination with physico-chemical analysis for assessing potential cytotoxic/genotoxic impacts of treated textile industry effluents reaching a major river (kelani rive ... | 2016 | 27209118 |
| mutagenic potential of fine wastes from dimension stone industry. | the industrial treatment of dimension stones, such as marbles and granites, includes a stage of plate polishing, in which resins and abrasives are used, producing a fine grained waste with high moisture content. these wastes pass through decantation tanks in order to separate the solid and liquid phases. until now, there is no knowledge about the mutagenic effects that this effluent can cause to organisms exposed to it. thus, this study evaluated the mutagenic potential of dimension stone polish ... | 2016 | 26685783 |