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mosquito species composition and plasmodium vivax infection rates for korean army bases near the demilitarized zone in the republic of korea, 2011.vivax malaria is a significant military and civilian health threat in northern republic of korea (rok). mosquito collections were performed at two rok army installations, paju near the demilitarized zone (dmz) using black light traps in 2011. the dmz, a 4 km wide border, is the northernmost point of the rok and separates the rok from the democratic people's republic of korea (dprk). anopheles spp. were identified by polymerase chain reaction and screened for plasmodium vivax sporozoites. of 4,35 ...201223243112
molecular detection of dirofilaria immitis, dirofilaria repens and setaria tundra in mosquitoes from germany.as a result of globalization and climate change, dirofilaria immitis and dirofilaria repens, the causative agents of dirofilariosis in europe, continue to spread from endemic areas in the mediterranean to northern and northeastern regions of europe where autochthonous cases of dirofilarial infections have increasingly been observed in dogs and humans. whilst d. repens was recently reported from mosquitoes in putatively non-endemic areas, d. immitis has never been demonstrated in mosquitoes from ...201424433279
reemergence of rift valley fever, mauritania, 2010.a rift valley fever (rvf) outbreak in humans and animals occurred in mauritania in 2010. thirty cases of rvf in humans and 3 deaths were identified. rvfv isolates were recovered from humans, camels, sheep, goats, and culex antennatus mosquitoes. phylogenetic analysis of isolates indicated a virus origin from western africa.024447381
human and animal dirofilariasis: the emergence of a zoonotic mosaic.dirofilariasis represents a zoonotic mosaic, which includes two main filarial species (dirofilaria immitis and d. repens) that have adapted to canine, feline, and human hosts with distinct biological and clinical implications. at the same time, both d. immitis and d. repens are themselves hosts to symbiotic bacteria of the genus wolbachia, the study of which has resulted in a profound shift in the understanding of filarial biology, the mechanisms of the pathologies that they produce in their hos ...022763636
circulation of dirofilaria repens and dirofilaria immitis in moldova.over the last two decades, a significant spread of dirofilariasis has been observed in eastern and central europe. however, data on the circulation of dirofilaria spp. in moldova were absent although direct neighbor states reported high incidence rates of human dirofilariasis.201627912786
the fauna and ecology of mosquitoes (diptera: culicidae) in western iran.the aim of this study was to obtain new data which would be valuable to develop programs for future planning of mosquito controls in in western iran.201426114143
lack of evidence for the presence of schmallenberg virus in mosquitoes in germany, 2011.in 2011, a novel orthobunyavirus of the simbu serogroup was discovered near the german-dutch border and named schmallenberg virus (sbv). so far, sbv genome has been detected in various field-collected culicoides species; however, other members of the simbu serogroup are also transmitted by mosquitoes.201425174354
molecular detection of setaria tundra (nematoda: filarioidea) and an unidentified filarial species in mosquitoes in germany.knowledge of the potential vector role of culicidae mosquitoes in germany is very scanty, and until recently it was generally assumed that they are not involved in the transmission of anthroponotic or zoonotic pathogens in this country. however, anticipated changes in the course of global warming and globalization may alter their status.201222236560
monitoring resistance to bacillus thuringiensis subsp. israelensis in the field by performing bioassays with each cry toxin separately.bacillus thuringiensis subsp. israelensis (bti) is increasingly used worldwide for mosquito control and is the only larvicide used in the french rhône-alpes region since decades. the artificial selection of mosquitoes with field-persistent bti collected in breeding sites from this region led to a moderate level of resistance to bti, but to relatively high levels of resistance to individual bti cry toxins. based on this observation, we developed a bioassay procedure using each bti cry toxin separ ...024037105
dirofilaria repens microfilariae in aedes vexans mosquitoes in slovakia.in this study, we screened field-caught mosquitoes for presence of dirofilaria spp. by using a polymerase chain reaction (pcr) assay. potential occurrence of dirofilaria repens and dirofilaria immitis microfilariae was examined in 3,600 mosquitoes of eight species (aedes vexans, aedes cinereus, aedes rossicus, culex pipiens, culiseta annulata, ochlerotatus sticticus, ochlerotatus cantans and ochlerotatus caspius) collected from five locations in two districts (kosice and trebisov) of eastern slo ...201323846240
dirofilaria in humans, dogs, and vectors in austria (1978-2014)-from imported pathogens to the endemicity of dirofilaria repens.dirofilaria repens and d. immitis are filarioid helminths with domestic and wild canids as main hosts and mosquitoes as vectors. both species are known to cause zoonotic diseases, primarily pulmonary (d. immitis), ocular (d. repens), and subcutaneous (d. repens) dirofilariosis. both d. immitis and d. repens are known as invasive species, and their distribution seems associated with climate change. until very recently, both species were known to be nonendemic in austria.201627196049
microsporidium infecting anopheles supepictus (diptera: culicidae) larvae.microsporidia are known to infect a wide variety of animals including mosquitoes (diptera: culicidae). in a recent study on the mosquito fauna of chahar mahal and bakhtiari province, at the central western part of iran, a few larvae of anopheles superpictus were infected with a microsporidium-resembled microorganism. current investigation deals with the identification of the responsible microorganism at the genus level.201627308299
aedes mosquito species in western saudi arabia.the aedes meigen (diptera: culicidae) mosquito species populations in the western region of saudi arabia, especially in and around jeddah, are increasing, therefore increasing susceptibility of humans to the dengue virus. an extensive survey was carried out for one year, and four species were identified with the help of different pictorial keys available. the identification was based on morphological characteristics of adult female aedes mosquitoes.201425373216
risk of canine and human exposure to dirofilaria immitis infected mosquitoes in endemic areas of italy.the occurrence of infections by dirofilaria immitis in canine and human populations depends on several factors linked to both the definitive and intermediate hosts. little data are available on the risk of human and dog exposure to d. immitis in endemic areas. data collected on dog- and human-bait traps in endemic areas of north-eastern italy were used to estimate the likelihood of a receptive host coming into contact with an infected vector.201323510597
vector-borne helminths of dogs and humans in europe.presently, 45% of the total human population of europe, as well as their domestic and companion animals, are exposed to the risk of vector-borne helminths (vbh) causing diseases. a plethora of intrinsic biological and extrinsic factors affect the relationship among helminths, vectors and animal hosts, in a constantly changing environment. although canine dirofilarioses by dirofilaria immitis and dirofilaria repens are key examples of the success of vbh spreading into non-endemic areas, another e ...201323324440
approaches to passive mosquito surveillance in the eu.the recent emergence in europe of invasive mosquitoes and mosquito-borne disease associated with both invasive and native mosquito species has prompted intensified mosquito vector research in most european countries. central to the efforts are mosquito monitoring and surveillance activities in order to assess the current species occurrence, distribution and, when possible, abundance, in order to permit the early detection of invasive species and the spread of competent vectors. as active mosquit ...201525567671
evolutionary and plastic responses of freshwater invertebrates to climate change: realized patterns and future potential.we integrated the evidence for evolutionary and plastic trait changes in situ in response to climate change in freshwater invertebrates (aquatic insects and zooplankton). the synthesis on the trait changes in response to the expected reductions in hydroperiod and increases in salinity indicated little evidence for adaptive, plastic, and genetic trait changes and for local adaptation. with respect to responses to temperature, there are many studies on temporal trait changes in phenology and body ...201324454547
identifying the main mosquito species in china based on dna barcoding.mosquitoes are insects of the diptera, nematocera, and culicidae families, some species of which are important disease vectors. identifying mosquito species based on morphological characteristics is difficult, particularly the identification of specimens collected in the field as part of disease surveillance programs. because of this difficulty, we constructed dna barcodes of the cytochrome c oxidase subunit 1, the coi gene, for the more common mosquito species in china, including the major dise ...201223071708
mosquito oviposition behavior and vector control.the burden of gene transfer from one mosquito generation to the next falls on the female and her eggs. the selection of an oviposition site that guarantees egg and larval survival is a critical step in the reproductive process. the dangers associated with ephemeral aquatic habitats, lengthy droughts, freezing winters, and the absence of larval nutrition makes careful oviposition site selection by a female mosquito extremely important. mosquito species exhibit a remarkable diversity of ovipositio ...201627869724
impact of mosquito bites on asexual parasite density and gametocyte prevalence in asymptomatic chronic plasmodium falciparum infections and correlation with ige and igg titers.an immunomodulatory role of arthropod saliva has been well documented, but evidence for an effect on plasmodium sp. infectiousness remains controversial. mosquito saliva may orient the immune response toward a th2 profile, thereby priming a th2 response against subsequent antigens, including plasmodium. orientation toward a th1 versus a th2 profile promotes igg and ige proliferation, respectively, where the former is crucial for the development of an efficient antiparasite immune response. here ...201222451520
recent outbreaks of rift valley fever in east africa and the middle east.rift valley fever (rvf) is an important neglected, emerging, mosquito-borne disease with severe negative impact on human and animal health. mosquitoes in the aedes genus have been considered as the reservoir, as well as vectors, since their transovarially infected eggs withstand desiccation and larvae hatch when in contact with water. however, different mosquito species serve as epizootic/epidemic vectors of rvf, creating a complex epidemiologic pattern in east africa. the recent rvf outbreaks i ...201425340047
mapping eastern equine encephalitis virus risk for white-tailed deer in michigan.eastern equine encephalitis (eee) is a mosquito-borne viral disease that is often fatal to humans and horses. some species including white-tailed deer and passerine birds can survive infection with the eee virus (eeev) and develop antibodies that can be detected using laboratory techniques. in this way, collected serum samples from free ranging white-tailed deer can be used to monitor the presence of the virus in ecosystems. this study developed and tested a risk index model designed to predict ...026494931
cardio-pulmonary parasitic nematodes affecting cats in europe: unraveling the past, depicting the present, and predicting the future.various cardio-pulmonary parasitic nematodes infecting cats have recently been fascinating and stimulating the attention of the academia, pharmaceutical companies, and veterinary practitioners. this is the case of the metastrongyloids: aelurostrongylus abstrusus and troglostrongylus brevior, the trichuroid: capillaria aerophila (syn. eucoleus aerophilus), and the filarioid: dirofilaria immitis. apparently, these parasites have been emerging in several european countries, thus, gaining an importa ...201426664917
effects of co-habitation between anopheles gambiae s.s. and culex quinquefasciatus aquatic stages on life history traits.the effective measures for the control of malaria and filariasis vectors can be achieved by targeting immature stages of anopheline and culicine mosquitoes in productive habitat. to design this strategy, the mechanisms (like biotic interactions with conspecifc and heterospecific larvae) regulating mosquito aquatic stages survivorship, development time and the size of emerging adults should be understood. this study explored the effect of co-habitation between an. gambiae s.s. and cx. quinquefasc ...201222321562
modelling hotspots of the two dominant rift valley fever vectors (aedes vexans and culex poicilipes) in barkédji, sénégal.climatic and environmental variables were used successfully by using models to predict rift valley fever (rvf) virus outbreaks in east africa. however, these models are not replicable in the west african context due to a likely difference of the dynamic of the virus emergence. for these reasons specific models mainly oriented to the risk mapping have been developed. hence, the areas of high vector pressure or virus activity are commonly predicted. however, the factors impacting their occurrence ...201626922792
statistical modeling of the abundance of vectors of west african rift valley fever in barkédji, senegal.rift valley fever is an emerging mosquito-borne disease that represents a threat to human and animal health. the exophilic and exophagic behavior of the two main vector in west africa (aedes vexans and culex poicilipes), adverse events post-vaccination, and lack of treatment, render ineffective the disease control. therefore it is essential to develop an information system that facilitates decision-making and the implementation of adaptation strategies. in east africa, rvf outbreaks are linked w ...201425437856
rift valley fever outbreak, southern mauritania, 2012.after a period of heavy rainfall, an outbreak of rift valley fever occurred in southern mauritania during september-november 2012. a total of 41 human cases were confirmed, including 13 deaths, and 12 rift valley fever virus strains were isolated. moudjeria and temchecket departments were the most affected areas.024447334
socio-economic impact of rift valley fever to pastoralists and agro pastoralists in arusha, manyara and morogoro regions in tanzania.rift valley fever (rvf) is a viral notifiable zoonotic disease primarily of domestic ruminants that causes significant socio-economic impacts. using the 2006-07 outbreak cases, this study aimed to establish the socio-economic impact of rvf and assessing knowledge, attitude and practice of livestock keepers towards controlling rvf in selected areas of tanzania. data were collected in arusha, manyara and morogoro regions using questionnaires, focus group discussions and in-depth interviews with ke ...201324255846
unexpected rift valley fever outbreak, northern mauritania.during september-october 2010, an unprecedented outbreak of rift valley fever was reported in the northern sahelian region of mauritania after exceptionally heavy rainfall. camels probably played a central role in the local amplification of the virus. we describe the main clinical signs (hemorrhagic fever, icterus, and nervous symptoms) observed during the outbreak.022000364
the temporal spectrum of adult mosquito population fluctuations: conceptual and modeling implications.an improved understanding of mosquito population dynamics under natural environmental forcing requires adequate field observations spanning the full range of temporal scales over which mosquito abundance fluctuates in natural conditions. here we analyze a 9-year daily time series of uninterrupted observations of adult mosquito abundance for multiple mosquito species in north carolina to identify characteristic scales of temporal variability, the processes generating them, and the representativen ...201425478861
host compatibility rather than vector-host-encounter rate determines the host range of avian plasmodium parasites.blood-feeding arthropod vectors are responsible for transmitting many parasites between vertebrate hosts. while arthropod vectors often feed on limited subsets of potential host species, little is known about the extent to which this influences the distribution of vector-borne parasites in some systems. here, we test the hypothesis that different vector species structure parasite-host relationships by restricting access of certain parasites to a subset of available hosts. specifically, we invest ...201323595266
stable transmission of dirofilaria repens nematodes, northern germany. 024447653
folk knowledge of invertebrates in central europe - folk taxonomy, nomenclature, medicinal and other uses, folklore, and nature conservation.there is scarce information about european folk knowledge of wild invertebrate fauna. we have documented such folk knowledge in three regions, in romania, slovakia and croatia. we provide a list of folk taxa, and discuss folk biological classification and nomenclature, salient features, uses, related proverbs and sayings, and conservation.201627729074
first detection of heartland virus (bunyaviridae: phlebovirus) from field collected arthropods.heartland virus (hrtv), the first pathogenic phlebovirus (family: bunyaviridae) discovered in the united states, was recently described from two missouri farmers. in 2012, we collected 56,428 ticks representing three species at 12 sites including both patients' farms. amblyomma americanum and dermacentor variabilis accounted for nearly all ticks collected. ten pools composed of deplete nymphs of a. americanum collected at a patient farm and a nearby conservation area were reverse transcription-p ...201323878186
preliminary results on the control of aedes spp. in a remote guatemalan community vulnerable to dengue, chikungunya and zika virus: community participation and use of low-cost ecological ovillantas for mosquito control.objective: to study the effectiveness of an integrated intervention of health worker training, a low-cost ecological mosquito ovitrap, and community engagement on aedes spp. mosquito control over 10 months in 2015 in an urban remote community in guatemala at risk of dengue, chikungunya and zika virus transmission. methods: we implemented a three-component integrated intervention consisting of: web-based training of local health personnel in vector control, cluster-randomized assignment of an eco ...201628105304
biologically informed individual-based network model for rift valley fever in the us and evaluation of mitigation strategies.rift valley fever (rvf) is a zoonotic disease endemic in sub-saharan africa with periodic outbreaks in human and animal populations. mosquitoes are the primary disease vectors; however, rift valley fever virus (rvfv) can also spread by direct contact with infected tissues. the transmission cycle is complex, involving humans, livestock, and multiple species of mosquitoes. the epidemiology of rvfv in endemic areas is strongly affected by climatic conditions and environmental variables. in this res ...201627662585
a survival and reproduction trade-off is resolved in accordance with resource availability by virgin female mosquitoes.the first 2-4 days after an anopheles gambiae female mosquito emerges are critical to her survival and reproductive success. yet, the order of behavioural events (mating, sugar feeding, blood feeding) during this time has received little attention. we discovered that among female cohorts sampled from emergence, sugar feeding had a higher probability than blood feeding of occurring first, and mating rarely occurred before a meal was taken. the night after emergence, 48% of females fed on sugar in ...021499504
complete genome sequence of two rift valley fever virus strains isolated from outbreaks in saudi arabia (2000) and kenya (2006 to 2007).the complete genome sequence, including the untranslated regions, of two rift valley fever virus (rvfv) strains isolated from mosquitoes that were collected from disease outbreaks in saudi arabia (2001) and kenya (2006 to 2007) were sequenced using next-generation sequencing technology.201627609913
experimental infection of calves by two genetically-distinct strains of rift valley fever virus.recent outbreaks of rift valley fever in ruminant livestock, characterized by mass abortion and high mortality rates in neonates, have raised international interest in improving vaccine control strategies. previously, we developed a reliable challenge model for sheep that improves the evaluation of existing and novel vaccines in sheep. this sheep model demonstrated differences in the pathogenesis of rift valley fever virus (rvfv) infection between two genetically-distinct wild-type strains of th ...201627223298
serological and molecular detection of dirofilaria species in stray dogs and investigation of wolbachia dna by pcr in turkey.dirofilaria immitis and dirofilaria repens are the most common species of filarial nematodes described in the dogs. a single-step multiplex pcr was applied to detect and differentiate simultaneously and unequivocally d. immitis and d. repens on dna extracted from canine peripheral blood and besides to detect the seroprevalance of d. immitis by elisa in elazig province, turkey. a pcr detection of the wolbachia, which plays an important role in d. immitis biology and contributes to the inflammator ...201628032096
the prevalence of dirofilaria repens in cats, healthy dogs and dogs with concurrent babesiosis in an expansion zone in central europe.dirofilaria repens is a mosquito-transmitted, filarial nematode parasitizing dogs, cats and other carnivores. recently, this parasite has spread in central europe, including poland. the aim of the present study was to estimate the prevalence of d. repens in cats and dogs in different regions of the country and to investigate the occurrence and consequences of co-infection with another fast-spreading vector-borne parasite, babesia canis.201627595920
vector competence of aedes vexans (meigen), culex poicilipes (theobald) and cx. quinquefasciatus say from senegal for west and east african lineages of rift valley fever virus.rift valley fever virus (rvfv; phlebovirus, bunyaviridae) is a mosquito-borne, zoonotic pathogen. in senegal, rvfv was first isolated in 1974 from aedes dalzieli (theobald) and thereafter from ae. fowleri (de charmoy), ae. ochraceus theobald, ae. vexans (meigen), culex poicilipes (theobald), mansonia africana (theobald) and ma. uniformis (theobald). however, the vector competence of these local species has never been demonstrated making hypothetical the transmission cycle proposed for west afric ...201626897521
modeling the impact of climate change on the dynamics of rift valley fever.a deterministic seir model of rift valley fever (rvf) with climate change parameters was considered to compute the basic reproduction number ℛ 0 and investigate the impact of temperature and precipitation on ℛ 0. to study the effect of model parameters to ℛ 0, sensitivity and elasticity analysis of ℛ 0 were performed. when temperature and precipitation effects are not considered, ℛ 0 is more sensitive to the expected number of infected aedes spp. due to one infected livestock and more elastic to ...201424795775
phylogenetic and syntenic data support a single horizontal transference to a trypanosoma ancestor of a prokaryotic proline racemase implicated in parasite evasion from host defences.proline racemase (prac) enzymes of trypanosoma cruzi (tcprac), the agent of chagas disease, and trypanosoma vivax (tvprac), the agent of livestock trypanosomosis, have been implicated in the b-cells polyclonal activation contributing to immunosuppression and the evasion of host defences. the similarity to prokaryotic prac and the absence in trypanosoma brucei and trypanosoma congolense have raised many questions about the origin, evolution, and functions of trypanosome prac (tryprac) enzymes.201525890302
low temperature induces embryonic diapause in the spider mite, eotetranychus smithi.the spider mite, eotetranychus smithi pritchard & baker (acari: tetranychidae), exhibits a facultative diapause that occurs at the egg stage. diapause was induced by low temperatures alone (≤ 17.5°c) and averted by high temperatures (≥ 20°c). photoperiod had little effect on diapause induction. this is the first example of temperature-induced diapause in spider mites. the diapause eggs became larger and darker (orange) than non- diapause eggs (white to pale yellow), suggesting that egg size and ...201425373215
rift valley fever outbreaks in mauritania and related environmental conditions.four large outbreaks of rift valley fever (rvf) occurred in mauritania in 1998, 2003, 2010 and 2012 which caused lots of animal and several human deaths. we investigated rainfall and vegetation conditions that might have impacted on rvf transmission over the affected regions. our results corroborate that rvf transmission generally occurs during the months of september and october in mauritania, similarly to senegal. the four outbreaks were preceded by a rainless period lasting at least a week fo ...201424413703
host-seeking behavior and arbovirus detection in mosquitoes of habahe county, xinjiang uigur autonomous region, china.mosquitoes in habahe county of xinjiang uigur autonomous region in china are considered a serious nuisance problem to local residents, but little is known of their role in enzootic disease. therefore, host-seeking behavior and virus detection in mosquitoes were investigated in this study. adult host-seeking mosquitoes were sampled using the centers for disease control and prevention (cdc) light traps operated at three locations in june through august 2008. nine traps were used at each location a ...201526675454
circulation of dirofilaria repens, setaria tundra, and onchocercidae species in hungary during the period 2011-2013.dirofilaria repens and recently dirofilaria immitis are known to be endemic in hungary. since there is no related research on dirofilaria carrier mosquito species from hungary, we conducted a three-year mosquito surveillance study between 2011 and 2013. during the study period we examined 23,139 female mosquitoes with a generic filaria-specific taqman pcr assay, and characterized them by sequencing a 500 bp segment of 12s rrna. an important result of our study was the detection of setaria tundra ...201526412142
potential mosquito (diptera:culicidae) vector of dirofilaria repens and dirofilaria immitis in urban areas of eastern slovakia.this paper follows the study from 2013 focused on the molecular screening of mosquitoes as vectors of dirofilaria spp. which provided the information on aedes vexans as a potential vector of dirofilaria repens in slovakia. current entomological and molecular research indicates that ae. vexans can participate also in the transmission of dirofilaria immitis within the region. using the standard pcr method, we examined 10,500 mosquitoes (ae. vexans, ae. rossicus, anopheles maculipennis s.l., ochler ...201526391170
first molecular identification of dirofilaria spp. (onchocercidae) in mosquitoes from serbia.dirofilariosis is a common and widespread veterinary health issue in several european countries with notable zoonotic potential. the causative agents are dirofilaria immitis and dirofilaria repens nematoda species which are transmitted by different mosquito vectors. similar to other mosquito-borne infections, the knowledge about mosquito species involved in disease transmission is crucial for the complex understanding of local transmission cycles. since there is no available data on mosquito spe ...201627193348
molecular investigation of francisella-like endosymbiont in ticks and francisella tularensis in ixodid ticks and mosquitoes in turkey.this study was carried out to investigate the molecular prevalence of francisella-like endosymbionts (fles) and francisella tularensis in ticks (acari: ixodidae) and mosquitoes in turkey. genomic dna pools were constructed from a total of 1477 adult hard ticks of rhipicephalus (rh.) annulatus, rh. turanicus, rh. sanguineus, rh. bursa, haemaphysalis (hae.) parva, hae. sulcata, hyalomma marginatum marginatum, h. anatolicum anatolicum, h. anatolicum excavatum, h. detritum detritum, h. dromedarii, d ...201626741324
genetic and phenotypic variation in central and northern european populations of aedes (aedimorphus) vexans (meigen, 1830) (diptera, culicidae).the floodwater mosquito aedes vexans can be a massive nuisance in the flood plain areas of mainland europe, and is the vector of tahyna virus and a potential vector of dirofilaria immitis. this epidemiologically important species forms three subspecies worldwide, of which aedes vexans arabiensis has a wide distribution in europe and africa. we quantified the genetic and phenotypic variation in ae. vexans arabiensis in populations from sweden (northern europe), hungary, and serbia (central europe ...201627232139
[genetic characterisation of powassan virus (powv) isolated from haemophysalis longicornis ticks in primorye and two strains of tick-borne encephalitis virus (tbev) (flaviviridae, flavivirus): alma-arasan virus (aav) isolated from ixodes persulcatus ticks in kazakhstan and malyshevo virus isolated from aedes vexans nipponii mosquitoes in khabarovsk kray].the complete genomes of the three tick-borne flaviviruses (genus flavivirus, fam. bunyaviridae) were sequenced: povassan virus (powv, strain leiv-3070prm, isolated from haemophysalis logicornis in primorsky krai, russia in 1977), alma-arasan virus (aav, strain leiv-1380kaz, isolated from ixodes persulcatus ticks in kazakhstan in 1977) and malyshevo virus (isolated from a pool of aedes vexans nipponii mosquitoes, in the khabarovsk krai, russia in 1978). it is shown that aav and malyshevo virus ar ...201425895206
mosquitoes (diptera: culicidae) in mauritania: a review of their biodiversity, distribution and medical importance.although mosquitoes (diptera: culicidae) are important disease vectors, information on their biodiversity in mauritania is scarce and very dispersed in the literature. data from the scientific literature gathered in the country from 1948 to 2016 were collected and analyzed. overall 51 culicid species comprising 17 anopheles spp., 14 aedes spp., 18 culex spp. and two mansonia spp. have been described in mauritania among which anopheles arabiensis, aedes vexans, culex poicilipes and culex antennat ...201728103955
prevalence of dirofilaria immitis (nematoda: filarioidea) in mosquitoes from northeast arkansas, the united states.a mosquito survey was conducted to identify which species of mosquitoes carry dirofilaria immitis (leidy) (nematoda: filarioidea), dog heartworm, in northeast arkansas. using polymerase chain reaction, mosquitoes were analyzed for d. immitis, dirofilaria repens railliet & henry, and acanthocheilonema dracunculoides cobbold. mosquitoes were collected from april to october 2009 using black light ultraviolet traps baited with dry ice. sixteen mosquito species were identified. d. immitis was identif ...201323926787
morphological studies on adult mosquitoes (diptera: culicidae) and first report of the potential zika virus vector aedes (stegomyia) unilineatus (theobald, 1906) in iran.beside numerous extensive studies on anophelinae mosquitoes of iran, little is known on aedes species in the country and existing reports are dispersed. the objective of this study was to identify adults of culicinae species occurring in the sistan and baluchestan province, southeast of iran, during 2012-2014. mosquito collections were carried out three times (may-june, september, october-november) in four counties by centers for diseases control (cdc) light traps and human landing catches outdo ...201628028709
specific detection of the floodwater mosquitoes aedes sticticus and aedes vexans dna in predatory diving beetles.floodwater mosquitoes (diptera: culicidae) are associated with periodically flooded wet meadows, marshes, and swamps in floodplains of major rivers worldwide, and their larvae are abundant in the shallow parts of flooded areas. the nuisance caused by the blood-seeking adult female mosquitoes motivates mosquito control. larviciding with bacillus thuringiensis israelensis is considered the most environmentally safe method. however, some concern has been raised whether aquatic predatory insects cou ...201524895318
bunched, the drosophila homolog of the mammalian tumor suppressor tsc-22, promotes cellular growth.transforming growth factor-beta1 stimulated clone-22 (tsc-22) is assumed to act as a negative growth regulator and tumor suppressor. tsc-22 belongs to a family of putative transcription factors encoded by four distinct loci in mammals. possible redundancy among the members of the tsc-22/dip/bun protein family complicates a genetic analysis. in drosophila, all proteins homologous to the tsc-22/dip/bun family members are derived from a single locus called bunched (bun).200818226226
obesity-blocking neurons in drosophila.in mammals, fat store levels are communicated by leptin and insulin signaling to brain centers that regulate food intake and metabolism. by using transgenic manipulation of neural activity, we report the isolation of two distinct neuronal populations in flies that perform a similar function, the c673a-gal4 and fruitless-gal4 neurons. when either of these neuronal groups is silenced, fat store levels increase. this change is mediated through an increase in food intake and altered metabolism in c6 ...019679073
tabanidae and other diptera on camel's hump vermont: ecological observations.a canopy trap and aerial nets led to finding 8 species of tabanidae. there was an abundance of calyptrate muscoid flies. camel's hump is in the green mountains of western new england, usa. discovering diptera on camel's hump involved sixteen visits over 40 years. upwards of 23 other diptera species are listed. habitats on the east side and above 762 m (2500 ft) elevation on camel's hump differ from the west slope but the boreal forest on both sides is influenced by cloud and fog precipitation on ...201122371675
combining hydrology and mosquito population models to identify the drivers of rift valley fever emergence in semi-arid regions of west africa.rift valley fever (rvf) is a vector-borne viral zoonosis of increasing global importance. rvf virus (rvfv) is transmitted either through exposure to infected animals or through bites from different species of infected mosquitoes, mainly of aedes and culex genera. these mosquitoes are very sensitive to environmental conditions, which may determine their presence, biology, and abundance. in east africa, rvf outbreaks are known to be closely associated with heavy rainfall events, unlike in the semi ...201222928055
modifying the nss gene to improve live-attenuated vaccine for rift valley fever. 023249225
identifying landscape features associated with rift valley fever virus transmission, ferlo region, senegal, using very high spatial resolution satellite imagery.dynamics of most of vector-borne diseases are strongly linked to global and local environmental changes. landscape changes are indicators of human activities or natural processes that are likely to modify the ecology of the diseases. here, a landscape approach developed at a local scale is proposed for extracting mosquito favourable biotopes, and for testing ecological parameters when identifying risk areas of rift valley fever (rvf) transmission. the study was carried out around barkedji villag ...201323452759
the transmission potential of rift valley fever virus among livestock in the netherlands: a modelling study.rift valley fever virus (rvfv) is a zoonotic vector-borne infection and causes a potentially severe disease. many mammals are susceptible to infection including important livestock species. although currently confined to africa and the near-east, this disease causes concern in countries in temperate climates where both hosts and potential vectors are present, such as the netherlands. currently, an assessment of the probability of an outbreak occurring in this country is missing. to evaluate the ...201323876054
rift valley fever virus mp-12 vaccine is fully attenuated by a combination of partial attenuations in the s, m, and l segments.rift valley fever (rvf) is a mosquito-borne zoonotic disease endemic to africa and characterized by a high rate of abortion in ruminants and hemorrhagic fever, encephalitis, or blindness in humans. rvf is caused by rift valley fever virus (rvfv; family bunyaviridae, genus phlebovirus), which has a tripartite negative-stranded rna genome (consisting of the s, m, and l segments). further spread of rvf into countries where the disease is not endemic may affect the economy and public health, and vac ...201525948740
distribution and mosquito hosts of chaoyang virus, a newly reported flavivirus from the republic of korea, 2008-2011.in total, 183,602 female culicine mosquitoes were captured by mosquito magnet, black light, and new jersey light traps, and manual aspiration of resting blood-fed mosquitoes, in the republic of korea from 2008 to 2011. culicine mosquitoes were identified to species, placed in pools of up to 30 mosquitoes each, and screened for flavivirus rna by using an sybr green i-based reverse transcription-polymerase chain reaction assay. thirty-two of the 8,199 pools assayed were positive by quantitative po ...201424724298
potential for dna-based identification of great lakes fauna: match and mismatch between taxa inventories and dna barcode libraries.dna-based identification of mixed-organism samples offers the potential to greatly reduce the need for resource-intensive morphological identification, which would be of value both to bioassessment and non-native species monitoring. the ability to assign species identities to dna sequences found depends on the availability of comprehensive dna reference libraries. here, we compile inventories for aquatic metazoans extant in or threatening to invade the laurentian great lakes and examine the avai ...201526199185
a systematic review of rift valley fever epidemiology 1931-2014.rift valley fever (rvf) is a mosquito-borne viral zoonosis that was first isolated and characterized in 1931 in kenya. rvf outbreaks have resulted in significant losses through human illness and deaths, high livestock abortions and deaths. this report provides an overview on epidemiology of rvf including ecology, molecular diversity spatiotemporal analysis, and predictive risk modeling.201526234531
dirofilaria repens: emergence of autochthonous human infections in the czech republic (case reports).human dirofilariasis is a zoonotic infection that continues to spread to previously unaffected areas of europe. in the south moravian region of the czech republic (cr), imported as well as autochthonous canine infections were recorded in the last decade, and parasite dna was detected in mosquitoes of aedes vexans. in the present paper, human dirofilaria infections are reported from the country for the first time.201627094256
mortality and pathology in birds due to plasmodium (giovannolaia) homocircumflexum infection, with emphasis on the exoerythrocytic development of avian malaria parasites.species of avian malaria parasites (plasmodium) are widespread, but their virulence has been insufficiently investigated, particularly in wild birds. during avian malaria, several cycles of tissue merogony occur, and many plasmodium spp. produce secondary exoerythrocytic meronts (phanerozoites), which are induced by merozoites developing in erythrocytic meronts. phanerozoites markedly damage organs, but remain insufficiently investigated in the majority of described plasmodium spp. avian malaria ...201627146519
dietary niche partitioning of euphaea formosa and matrona cyanoptera (odonata: zygoptera) on the basis of dna barcoding of larval feces.odonate larvae are commonly considered opportunistic general predators in freshwater ecosystems. however, the dietary breadth of most odonate larvae in forest streams is still poorly documented. we characterized the prey species and estimated the level of dietary niche overlap of two damselflies, euphaea formosa hagen 1869 and matrona cyanoptera hämäläinen and yeh, 2000 in a forest stream of central taiwan on the basis of dna barcoding of larval feces. a collection of 23 successfully identified ...201627432350
insect-specific flaviviruses in aedes mosquitoes in greece.mosquitoes of the genus aedes are known vectors of pathogenic flaviviruses, and insect-specific flaviviruses (isfs) have been detected in members of this genus in numerous parts of the world. in order to gain insight into whether aedes mosquitoes in greece are infected by flaviviruses, 1173 aedes spp. mosquitoes collected in 2010 and 2012 were grouped in 53 pools and tested by rt nested pcr using flavivirus generic primers. eight pools (15.09 %) were found to be pcr positive: five pools (5/53, 9 ...201627198866
predicting rift valley fever inter-epidemic activities and outbreak patterns: insights from a stochastic host-vector model.rift valley fever (rvf) outbreaks are recurrent, occurring at irregular intervals of up to 15 years at least in east africa. between outbreaks disease inter-epidemic activities exist and occur at low levels and are maintained by female aedes mcintoshi mosquitoes which transmit the virus to their eggs leading to disease persistence during unfavourable seasons. here we formulate and analyse a full stochastic host-vector model with two routes of transmission: vertical and horizontal. by applying br ...201628002417
a regional model for malaria vector developmental habitats evaluated using explicit, pond-resolving surface hydrology simulations.dynamical malaria models can relate precipitation to the availability of vector breeding sites using simple models of surface hydrology. here, a revised scheme is developed for the vectri malaria model, which is evaluated alongside the default scheme using a two year simulation by hydremats, a 10 metre resolution, village-scale model that explicitly simulates individual ponds. despite the simplicity of the two vectri surface hydrology parametrization schemes, they can reproduce the sub-seasonal ...201627003834
bunyavirus-vector interactions.the bunyaviridae family is comprised of more than 350 viruses, of which many within the hantavirus, orthobunyavirus, nairovirus, tospovirus, and phlebovirus genera are significant human or agricultural pathogens. the viruses within the orthobunyavirus, nairovirus, and phlebovirus genera are transmitted by hematophagous arthropods, such as mosquitoes, midges, flies, and ticks, and their associated arthropods not only serve as vectors but also as virus reservoirs in many cases. this review present ...201425402172
evaluation of vegetation treatment with lambda-cyhalothrin to control aedes vexans and simulium maculatum in northern china.the mosquito aedes vexans and black fly simulium maculatum are important blood-sucking pests in northern china. control of these pests using current methods is difficult because of complex larval developmental sites. in the present study, lambda-cyhalothrin was applied as a barrier spray to vegetation and various structures surrounding human dwellings for control against the adults of each species. the barrier spray resulted in an 88.2-99.4% reduction in ae. vexans abundance and a 67.8-89.6% red ...201425843101
characterization of a novel circo-like virus in aedes vexans mosquitoes from germany: evidence for a new genus within the family circoviridae.over recent decades, metagenomic studies have expanded the number of newly described, often unclassified, viruses within the family circoviridae. using broad-spectrum circovirus and cyclovirus pcrs, we characterized a novel circo-like virus in aedes vexans mosquitoes from germany whose main putative orfs shared very low amino acid identity with those of previously characterized circoviruses and cycloviruses. phylogenetic and genetic distance analysis revealed that this new virus species defined, ...201525535324
description of the first cryptic avian malaria parasite, plasmodium homocircumflexum n. sp., with experimental data on its virulence and development in avian hosts and mosquitoes.for over 100 years studies on avian haemosporidian parasite species have relied on similarities in their morphology to establish a species concept. some exceptional cases have also included information about the life cycle and sporogonic development. more than 50 avian plasmodium spp. have now been described. however, pcr-based studies show a much broader diversity of haemosporidian parasites, indicating the possible existence of a diverse group of cryptic species. in the present study, using bo ...201525449950
dirofilaria repens railliet et henry, 1911--a new parasite acclimatized in poland.dirofilariosis caused by the dirofilaria repens nematodes is widely dispersed in southern europe, asia and africa among dogs, cats, other carnivores and occasionally, humans. the first case of d. repens infection, found in poland in 2007, concerned humans. in 2009, dirofilariosis was first registered in dogs in 3 focuses in central poland, warsaw, pruszków and zyrardów, whose range grew considerably with the subsequent identification of d. repens microfilariae in 119 dogs in warsaw and 18 distri ...201424930244
zoonotic dirofilaria repens (nematoda: filarioidea) in aedes vexans mosquitoes, czech republic.the surveillance of vectors for arthropod-borne pathogens is nowadays an important tool in surveillance programmes throughout europe. whereas many studies have been performed to screen arthropods for viruses or bacterial pathogens, only limited information is available concerning the geographical distribution and vector range of pathogenic filariae in central europe. to consider the prevalence of filarial parasites in mosquito vectors, we performed a molecular survey of mosquitoes for filarial d ...201425346197
genetic diversity and differentiation between palearctic and nearctic populations of aedimorphus (=aedes) vexans (meigen, 1830) (diptera, culicidae).genetic diversity was studied at allozyme loci in two palearctic and one nearctic population of aedimorphus (=aedes) vexans, a species of public health and veterinary importance. the population from serbia was the most polymorphic (p= 35%) with the highest observed heterozygosity (h(o) = 0.027). the lowest observed heterozygosity (h(o) = 0.010) was obtained for the nearctic population. all analyses based on individual (structure analysis) and population level (pairwise f(st), nm values, amova, n ...201323701620
canine dirofilariosis endemic in central europe-10 years of epidemiological study in slovakia.the study presents the comprehensive results of a detailed epidemiological study on canine dirofilariosis in slovakia, central europe. more than 4000 dogs were investigated, and several epidemiological factors were considered. the mean prevalence in individual regions ranged from 2.0 % in northern slovakia to more than 25.0 % in the south-western part of the country, with the nematode dirofilaria repens confirmed as the dominant causative agent. canine dirofilariosis occurred more often in anima ...201627021185
prevalence of dirofilaria immitis (spirurida: onchocercidae) infection in aedes, culex, and culiseta mosquitoes from north san joaquin valley, ca.canine heartworm is one of the most serious infections primarily affecting domestic dogs but will also infect cats and wild canids. to evaluate the potential of mosquitoes as vectors of dog heartworm, dirofilaria immitis (leidy) in san joaquin county, ca, we collected mosquitoes in 2011 and analyzed for infection with heartworm by using polymerase chain reaction. of 3,000 mosquito pools (total number of specimens = 36,554), d. immitis dna was detected in 97 pools of seven species, and the overal ...201324843938
detection of mosquito-only flaviviruses in europe.the genus flavivirus, family flaviviridae, includes a number of important arthropod-transmitted human pathogens such as dengue viruses, west nile virus, japanese encephalitis virus and yellow fever virus. in addition, the genus includes flaviviruses without a known vertebrate reservoir, which have been detected only in insects, particularly in mosquitoes, such as cell fusing agent virus, kamiti river virus, culex flavivirus, aedes flavivirus, quang binh virus, nakiwogo virus and calbertado virus ...201222377581
potential for mosquitoes (diptera: culicidae) from florida to transmit rift valley fever virus.we evaluated aedes atlanticus dyar and knab, aedes infirmatus dyar and knab, aedes vexans (meigen), anopheles crucians wiedemann, coquillettidia perturbans (walker), culex nigripalpus theobald, mansonia dyari belkin, heinemann, and page, and psorophora ferox (von humboldt) from florida to determine which of these species should be targeted for control should rift valley fever virus (rvfv) be detected in north america. female mosquitoes that had fed on adult hamsters inoculated with rvfv were inc ...201324180117
a novel flavivirus detected in two aedes spp. collected near the demilitarized zone of the republic of korea.flaviviruses comprise a large and diverse group of positive-stranded rna viruses, including tick-, mosquito- and unknown-vector-borne flaviviruses. a novel flavivirus was detected in pools of aedes vexans nipponii (n=1) and aedes esoensis (n=3) collected in 2012 and 2013 near the demilitarized zone (dmz), republic of korea (rok). phylogenetic analyses of the ns5, e gene and complete polyprotein coding sequence (cds) showed that the novel virus fell within the aedes-borne flaviviruses (abfvs), wi ...201728555540
worthy of their name: how floods drive outbreaks of two major floodwater mosquitoes (diptera: culicidae).an understanding of how climate variables drive seasonal dynamics of mosquito populations is critical to mitigating negative impacts of potential outbreaks, including both nuisance effects and risk of mosquito-borne infectious disease. here, we identify climate variables most affecting seasonal dynamics of two major floodwater mosquitoes, aedes vexans (meigen, 1830) and aedes sticticus (meigen, 1838) (diptera: culicidae), along the lower courses of the dyje river, at the border between the czech ...201424605456
an overview of mosquitoes and emerging arboviral infections in the zagreb area, croatia.mosquito control in the zagreb area has been conducted for many years, whereas the fauna has only been investigated in the last 20 years. so far 30 mosquito species have been detected in the city area. culex pipiens form molestus is the dominant mosquito species in indoor breeding sites. in forested areas and areas exposed to flooding, the active period is early spring and the dominant species are ochlerotatus sticticus, ochlerotatus cantans, ochlerotatus geniculatus and aedes vexans. the eudomi ...201628036307
mosquito capture rate using co2-baited traps in relation to distance from water and height: implications for avian disease transmission.accumulating evidence suggests that enzootic transmission of pathogens such as west nile virus (wnv) by mosquitoes is governed by host-bird interactions, including mosquito preferences for specific species and developmental stages of host birds, host bird availability, and host defensive behavior. here, we examined how the attack rate of five mosquito species in southern california was influenced by the position of co2-baited traps in relation to distance from water and trap height. we identifie ...201627493249
american aedes vexans mosquitoes are competent vectors of zika virus.abstractstarting in 2013-2014, the americas have experienced a massive outbreak of zika virus (zikv) which has now reached at least 49 countries. although most cases have occurred in south america and the caribbean, imported and autochthonous cases have occurred in the united states. aedes aegypti and aedes albopictus mosquitoes are known vectors of zikv. little is known about the potential for temperate aedes mosquitoes to transmit zikv. aedes vexans has a worldwide distribution, is highly abun ...201728719283
potential of a northern population of aedes vexans (diptera: culicidae) to transmit zika virus.zika virus is an emerging arbovirus of humans in the western hemisphere. with its potential spread into new geographical areas, it is important to define the vector competence of native mosquito species. we tested the vector competency of aedes vexans (meigen) from the lake agassiz plain of northwestern minnesota and northeastern north dakota. aedes aegypti (l.) was used as a positive control for comparison. mosquitoes were fed blood containing zika virus and 2 wk later were tested for viral inf ...201728499036
host preferences in host-seeking and blood-fed mosquitoes in switzerland.the avian zoonotic agent for west nile virus (wnv) can cause neuroinvasive disease in horses and humans and is expanding its range in europe. analyses of the risk for transmission to these hosts in non-endemic areas are necessary. host preferences of mosquitoes (diptera: culicidae), the main vectors of wnv, were determined in switzerland using animal-baited trap (horse, chickens) experiments at a natural and a periurban site. this was undertaken on four occasions during may-september 2014. in ad ...201626685926
mosquito survey during west nile virus outbreak 2012 in northeast croatia.during the august and september 2012, seven human cases of the west nile neuro-invasive disease were reported in croatia. medical entomology research on a potential vectors during the outbreak was supported by the ministry of health. a mosquito survey has been done in 64 sites in three eastern croatian counties (osijek-baranja county, vukovar-srijem county and in brod-posavina county). dry ice baited cdc traps were used for mosquito sampling in a period from the 10th to 25th september 2012. a to ...201425144969
equine seroprevalence rates as an additional indicator for a more accurate risk assessment of the west nile virus transmission.the west nile virus (wnv) is a zoonotic arbovirus that has recently been causing outbreaks in many countries in southern and central europe. in 2012, for the first time, it caused an outbreak in eastern croatia with total of 7 human clinical cases. with an aim of assisting public health personnel in order to improve survey protocols and vector control, the high risk areas of the wnv transmission were estimated and mapped. the study area included cities of osijek and slavonski brod and 8 municipa ...201324308242
ecological niche modelling of potential west nile virus vector mosquito species and their geographical association with equine epizootics in italy.in italy, west nile virus (wnv) equine outbreaks have occurred annually since 2008. characterizing wnv vector habitat requirements allows for the identification of areas at risk of viral amplification and transmission. maxent-based ecological niche models were developed using literature records of 13 potential wnv italian vector mosquito species to predict their habitat suitability range and to investigate possible geographical associations with wnv equine outbreak occurrence in italy from 2008 ...201424121802
evaluation of methods for collecting blood-engorged mosquitoes from habitats within a wildlife refuge.mortality of american white pelican (pelecanus erythrorhynchos) chicks attributed to west nile virus (wnv) prompted field studies on the bionomics of mosquitoes on a wildlife refuge in northern montana. one component of these studies was to identify blood meal sources for culex tarsalis, the primary vector of wnv in the region, and the potential bridge vectors aedes vexans and culiseta inornata. to accomplish this, 3 methods were evaluated to collect bloodfed mosquitoes: a gasoline powered aspir ...201323923324
mosquito and west nile virus surveillance in northeast montana, u.s.a., 2005 and 2006.mosquito and west nile virus (wnv) surveillance was conducted on a national wildlife refuge in northeast montana in 2005 and 2006, during which outbreaks of wnv in a colony of american white pelicans (pelecanus erythrorhynchos gmelin) (pelecaniformes: pelecanidae) resulted in juvenile mortality rates of ∼ 31%. both years, floodwater species ochlerotatus dorsalis (meigen) (diptera: culicidae), aedes vexans (meigen) (diptera: culicidae) and ochlerotatus flavescens (muller) (diptera: culicidae) com ...201423647177
species composition and seasonal distribution of mosquito larvae (diptera: culicidae) in southern new jersey, burlington county.a total of 36,495 larvae consisting of 45 species from 11 genera were collected from 7,189 sites from southern new jersey, burlington county between the months of march and october, 2001-2014. density and seasonal distribution were determined among natural and artificial habitat. the most dominant species collected from natural habitat was aedes vexans (meigen) followed by ochlerotatus canadensis canadensis (theobald), culex restuans theobald, culex pipiens l., and culex territans walker. the mo ...201526336214
hyperparasitism of mosquitoes by water mite larvae.hyperparasitism of ectoparasitic water mite larvae on mosquitoes is still a neglected relationship and was investigated only in a few studies. we analysed 2313 female mosquitoes from six different sampling localities with regard to their degree of parasitism with water mite larvae. in total, we found 38 mosquito individuals parasitized by 93 water mite larvae, ranging from 1 to 12 larvae per mosquito. water mite larvae detected are members of the two species parathyas cf. barbigera (n = 92) and ...201525899329
geographic distribution of wolbachial infections in mosquitoes from thailand.members of the genus wolbachia are inherited intracellular bacterial endosymbionts that infect a diverse range of arthropods. here i report the results of a survey of these endosymbionts in different mosquito species from six geographic regions of northern, northeastern, western, central, eastern and southern thailand. using gene amplification assays with wsp and groe gene primers, wolbachiae were detected in 999 mosquitoes representing 28 species of 1622 specimens collected representing 74 spec ...201323660513
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