| asthmatic reactions after nasal allergen provocation. | three bronchoconstrictive responses associated with acute rhinitis and occurring in two patients were observed among 400 nasal allergen challenges. causative substances were birch pollen, dermatophagoides pteronyssinus and hsa-conjugated pyromellitic dianhydride. both subjects showed bronchial hyperreactivity and immediate-type sensitization to these allergens. it is concluded that bronchoobstruction nearly occurs in nasal allergen challenges. the underline pathophysiological mechanism remains u ... | 1996 | 8966371 |
| atopy and nonspecific bronchial responsiveness. a population-based assessment. spanish group of the european community respiratory health survey. | the relative importance of specific immunoresponse in explaining nonspecific bronchial responsiveness (br) has scarcely been examined. we provide quantitative estimates of the proportion of br attributable to specific atopy to different common aeroallergens. we used data from a cross-sectional study on a random sample of the general population 20 to 44 yr of age from five spanish areas. there were 1,816 participants who performed a methacholine challenge and had atopy assessed. br was defined as ... | 1996 | 8970347 |
| false-positive skin prick test responses to commercially available dog dander extracts caused by contamination with house dust mite (dermatophagoides pteronyssinus) allergens. | in an outpatient population, a high frequency of positive skin prick test responses to dog dander was found in the absence of detectable ige to dog dander in the rast. the majority of these patients were sensitized to house dust mites (dermatophagoides pteronyssinus) and had no obvious dog-related allergic symptoms. these findings prompted us to investigate whether dog dander skin test preparations are contaminated with house dust mite allergens in amounts sufficient to cause false-positive skin ... | 1996 | 8977501 |
| prevalence of respiratory symptoms, bronchial hyperresponsiveness and atopy among adults: west and east germany. | the prevalence of respiratory symptoms, atopic sensitization and bronchial hyperresponsiveness was compared in a random sample of adults, 20-44 yrs of age, in two cities in west and east germany, hamburg and erfurt, respectively. there were much higher levels of outdoor air pollution due to sulphur dioxide and suspended particulates in erfurt, and major differences in living conditions during the last 40 yrs. within the european respiratory health survey, a short questionnaire was answered by 3, ... | 1996 | 8980967 |
| effect of allergen specific immunotherapy (it) on natural killer cell activity (nk), ige, ifn-gamma levels and clinical response in patients with allergic rhinitis and asthma. | allergen specific immunotherapy (it) has been widely used for many years as a specific treatment of allergic diseases. a variety of changes in immunological parameters have been described but it still remains uncertain as to which of them is responsible for the improvement of symptoms. the aim of our study was to evaluate the effect of it on natural killer (nk) cell activity, il-4, ifn-gamma, ige levels and skin test reactivity in addition to clinical efficacy. thirty-one patients with allergic ... | 1996 | 9015776 |
| the distribution of total and specific serum ige in the european community respiratory health survey. | variations in the prevalence of atopy could provide important clues to the etiology of atopy and asthma. although estimates of prevalence are available from different studies, a lack of standardization makes comparisons difficult. | 1997 | 9058686 |
| allergic and nonallergic interactions between house dust mite allergens and airway mucosa. | asthma and allergy are extremely frequent diseases, affecting 5-10% and 30% of the population, respectively. the prevalence of asthma has increased in many developed countries, which may be due to several factors, including increased exposure to house dust mite (hdm) allergens. hdm to which humans are most frequently sensitized are dermatophagoides pteronyssinus, dermatophagoides farinae, and euroglyphus maynei. these mites multiply in carpets, bedding and upholstered furniture in a hot and humi ... | 1997 | 9073012 |
| allergen skin test reactivities among asthmatic thai children. | skin prick tests with 14 selected local aeroallergens were performed on 100 asthmatic children aged 0-16 years attending the pediatric allergy clinic, faculty of medicine, siriraj hospital. the 14 allergens included dermatophagoides farinae, dermatophagoides pteronyssinus, american cockroach (periplaneta americana), johnson grass (sorghum halopense), cladosporium clarosporoides, alternaria tenuis, penicillium mix, aspergillus mix, cat dander, dog dander, milk, egg white, soy and shrimp. positive ... | 1997 | 9078689 |
| cleavage of the low-affinity receptor for human ige (cd23) by a mite cysteine protease: nature of the cleaved fragment in relation to the structure and function of cd23. | der p i, a cysteine protease representing a major allergen of the house dust mite dermatophagoides pteronyssinus, has recently been shown to cleave cd23 from the surface of cultured human b cells (rpmi 8866 b cell line). we have now undertaken a detailed investigation of cd23 cleavage by der p i. we demonstrate that der p i cleaves cd23 at two sites (ser155-ser156 and glu298-ser299) to produce a 17-kda fragment containing the lectin domain and only part of the c-terminal tail. no such effect was ... | 1997 | 9079796 |
| stimulation of blood mononuclear cells of atopic children with the relevant allergen induces the release of eosinophil chemotaxins such as il-3, il-5, and gm-csf. | peripheral blood mononuclear cells (pbmc) from 10 atopic asthmatic children (atopics), sensitized to dermatophagoides pteronyssinus (dp), and from 5 nonatopic healthy children (controls) were stimulated with dp extract or with birch extract (be). after 6 days we tested the supernatant's (sn) chemotactic activity toward purified blood eosinbnophils and t-lymphocyte proliferation. dp induced a statistically significant t-cell proliferation from atopics as compared to controls (p < 0.05), which cor ... | 1997 | 9088301 |
| allergen content in dust from homes and schools in northern norway in relation to sensitization and allergy symptoms in schoolchildren. | previous studies have shown a high prevalence of atopic diseases among school children in the community of sør-varanger. moreover, animal dander followed by pollen and house dust mite, were the most common allergens in skin prick tests. | 1997 | 9088651 |
| asthma and its relation with dermatophagoides pteronyssinus and dermatophagoides farinae in andean altitudes (quito, ecuador). | previous investigations in alpine altitudes suggest a very low etiopathogenic relation between house-dust mites and bronchial asthma in these geographical zones. our purpose was to analyze the relationship between asthma and sensitization to the house-dust mites d. pteronyssinus and d. farinae in andean altitudes. we studied 587 asthmatic patients, residents in quito, ecuador, (2800 m above sea level), ranging in age from 8 months to 84 years. the patients were divided into five age groups. we i ... | 1997 | 9093934 |
| [the isolation and characteristics of a chemically modified allergen (allergoid) from dermatophagoides pteronyssinus mites]. | chemically modified allergens (allergoids) were obtained from house dust mites (dermatophagoides pteronyssinus). the allergenicity of allergoids in comparison with commercial mite allergen was determined by the skin prick test and by the in vitro test of degranulation of passively sensitized rat mast cells. changes in allergoid molecules were determined with the use of gel filtration in a column packed with sephadex g-75, isoelectric focusing in polyacrylamide gel, the determination of the conce ... | 1996 | 9103081 |
| allergen challenge of passively sensitized human bronchi alters m2 and beta2 receptor function. | we investigated whether antigen challenge may alter m2 and beta2 receptor function in isolated passively sensitized human bronchi. bronchial rings (2-4 mm internal diameter) were obtained from 12 patients. passive sensitization was induced by serum containing high ige levels to dermatophagoides pteronyssinus and dermatophagoides farinae. rings from six patients were used to study m2 receptor function by incubating with cumulatively increasing concentrations (10(-8) m to 10(-4) m) of pilocarpine ... | 1997 | 9105059 |
| rush immunotherapy results in allergen-specific alterations in lymphocyte function and interferon-gamma production in cd4+ t cells. | allergen immunotherapy results in a number of changes in clinical, inflammatory, and immunologic parameters. however, the basis for the specificity of this form of therapy is unknown, especially in the context of changes in t- and b-lymphocyte function after desensitization to specific allergens. | 1997 | 9111499 |
| il-5 secretion by allergen-stimulated cd4+ t cells in primary culture: relationship to expression of allergic disease. | il-5-producing allergen-specific t cells are thought to play a prominent role in the pathogenesis of allergic inflammation. we hypothesized that t cell allergen-driven il-5 synthesis is elevated in patients with atopic disease as compared with that in atopic patients free of disease and nonatopic control subjects. | 1997 | 9111503 |
| prenatal allergen contact with milk proteins. | cellular proliferation to various allergens (dermatophagoides pteronyssinus, beta-lactoglobulin, bovine serum albumin, ovalbumin) has been found in cord blood cells. whether this reflects a sensitization during foetal life is uncertain. | 1997 | 9117877 |
| feather mites are potentially an important source of allergens for pigeon and budgerigar keepers. | previous studies on allergy to feathers have not addressed whether organisms living on feathers (mites, lice, moulds) are a source of allergens. | 1997 | 9117882 |
| evaluating the factors that relate to asthma severity in adolescents. | over the past 5 years, we have been engaged in a cross-sectional evaluation of risk factors for higher asthma severity in adolescents aged 13-18. all recruitment takes place through public and private schools. the sample from which our current findings are derived includes 151 adolescents covering a wide spectrum of asthma severity and socioeconomic status (ses) and representing both african american and caucasians. an asthma severity instrument has been developed and validated for the purpose o ... | 1997 | 9130491 |
| the sweat of patients with atopic dermatitis contains specific ige antibodies to inhalant allergens. | we have investigated levels of total and specific ige against inhalant allergens in the sweat of 15 patients with atopic dermatitis, 10 patients with allergic rhinitis and high levels of specific ige in the serum, and five patients with psoriasis without atopy as controls, by means of various commercial methods such as fluorescence immunoassay, nephelometry, chemiluminescence assay, enzyme immunoassay and the radioallergosorbent test. total ige and specific ige antibodies were detectable in the ... | 1996 | 9136153 |
| house dust mite allergen levels in public places in new zealand. | house dust mite allergens are a risk factor for asthma in new zealand, and levels in domestic dwellings have been found to be high compared with levels in most other countries. studies in other countries have demonstrated lower levels of dermatophagoides pteronyssinus allergens in public places compared with levels in domestic dwellings. | 1997 | 9155822 |
| transfer of atopy following bone marrow transplantation. | bone marrow transplantation is an optimal treatment of acute leukemia and aplastic anemia. allergic manifestations in recipients long after bone marrow transplantation have been reported. a case involving transfer of atopy manifested as food allergy-induced-atopic dermatitis is reported. | 1997 | 9164366 |
| rapid expression of the cd69 antigen on t cells and natural killer cells upon antigenic stimulation of peripheral blood mononuclear cell suspensions. | the cd69 antigen has been identified as the earliest activation marker on the surface of cytokine- or mitogen-activated lymphocytes. the expression of this molecule may be a useful early marker of antigen- or allergen-specific activation of lymphocytes in vitro. we evaluated the expression of the cd69 and cd25 antigens on antigen- or allergen-stimulated lymphocytes and the proliferative responses as detected by thymidine incorporation. peripheral blood mononuclear cells (pbmc) of allergic patien ... | 1997 | 9188933 |
| il-13 production by allergen-stimulated t cells is increased in allergic disease and associated with il-5 but not ifn-gamma expression. | interleukin-13 (il-13) shares many, but not all, of the properties of the prototypic t-helper type 2 (th2) cytokine il-4, but its role in allergen-driven t-cell responses remains poorly defined. we hypothesized that allergen stimulation of peripheral blood t cells from patients with atopic disease compared with non-atopic controls results in elevated il-13 synthesis in the context of a 'th2-type' pattern. freshly isolated peripheral blood mononuclear cells (pbmc) obtained from sensitized atopic ... | 1997 | 9203965 |
| the most common phenotypes of sensitization to inhalant allergens in childhood. | atopic individuals are frequently sensitized to a limited number of certain allergens, although most of them are exposed to multiple inhalant allergens in daily life. | 1997 | 9208185 |
| functional cd86 (b7-2/b70) is predominantly expressed on langerhans cells in atopic dermatitis. | recently, we reported the functional expression of cd86 on cultured human langerhans cells derived from normal epidermis. in the present study, we investigated the expression and function of co-stimulatory molecules in the pathogenesis of atopic dermatitis. in immunohistochemical analysis, cd80 and/or cd86 were detected on dendritic-shaped cells not only in the epidermis but also in the dermis in the inflammatory lesions of atopic dermatitis (n = 12). cd80 was expressed in only five cases (42%), ... | 1997 | 9217814 |
| determination of the n- and c-terminal sequences required to bind human ige of the major house dust mite allergen der f 2 and epitope mapping for monoclonal antibodies. | b cell epitopes of the major house dust mite allergen der f 2 from dermatophagoides farinae were analysed using deletion mutants of der f 2 expressed as fusion proteins in escherichia coli. the reactivities of these partial der f 2 molecules to human anti-mite ige antibodies in atopic patients and to murine anti-der f2 monoclonal antibodies (mabs) were examined by immunoblotting. a c-terminal deletion mutant of der f 2, 1-123, had almost the same reactivity to human ige as the whole der f 2 (1-1 ... | 1997 | 9224967 |
| prevalence of wheeze and asthma and relation to atopy in urban and rural ethiopia. | asthma and allergy in developing countries may be associated with adoption of an urbanised "western" lifestyle. we compared the rates of asthma symptoms and atopy in urban populations in jimma, southwest ethiopia, at an early stage of economic development with those among the population of remote, rural, subsistence areas, and assessed the potential role of environmental aetiological factors leading to the differences. | 1997 | 9228959 |
| house dust mite and cat allergen levels in domestic dwellings in christchurch. | to quantify the levels of dermatophagoides pteronyssinus allergen (der p i) and felis domesticus allergen (fel d i) in domestic dwellings in christchurch and to assess possible relationships with housing characteristics. | 1997 | 9236806 |
| prevalence of childhood asthma based on questionnaires and methacholine bronchial provocation test in korea. | in most epidemiological survey studies, only subjective symptoms and past medical history of asthma have been used as diagnostic criteria. even though a questionnaire survey can be performed in a large population study at low cost, limitations such as lack of objectivity and poor predictability in non-specific bronchial hyperresponsiveness cannot be avoided. | 1997 | 9249268 |
| adhesion molecules in atopic dermatitis: upregulation of alpha6 integrin expression in spontaneous lesional skin as well as in atopen, antigen and irritative induced patch test reactions. | atopic dermatitis is a lymphocyte-mediated skin disease. we studied the expression of the adhesion molecule alpha6 integrin by immunohistochemistry in spontaneous atopic inflammation as well as during the eliciting phase of atopen (dermatophagoides pteronyssinus), antigen (nickel sulfate) and irritative (anthralin) induced patch test reactions in atopic skin. results were compared with nickel sulfate patch test reactions in normal skin. a role of the alpha6 integrin, expressed at the luminal sid ... | 1997 | 9250597 |
| exposure to indoor allergens in early infancy and sensitization. | indoor allergens play a major role both in sensitization and as triggers of asthma in children. the relationship between allergen exposure and sensitization to cats, dogs, and mites was studied prospectively in 100 newborn babies with a history of allergy in both parents. | 1997 | 9275137 |
| identification of the domestic mite fauna of puerto rico. | this study was conducted to identify the domestic mite fauna of puerto rico. a total of 57 dust samples were collected from mattresses in homes of 11 cities on the island. the analysis of the samples revealed that 73.70% of the mattress samples had at least one mite species. the identified species include: dermatophagoides pteronyssinus (45.6%), blomia tropicalis (31.6%), cheyletus sp. (19.3%), dermatophagoides farinae (17.5%), euroglyphus maynei (5.3%), dermatophagoides sibonei (1.8%), dermatop ... | 1997 | 9285987 |
| mould-devouring mites differ in guanine excretion from dust-eating acari, a possible error source in mite allergen exposure studies. | measurement of guanine in dust proved a good assessment of mite allergen exposure. | 1997 | 9291290 |
| immune memory in cd4+ cd45ra+ t cells. | this study addresses the question of whether human peripheral cd4+ cd45ra+ t cells possess antigen-specific immune memory. cd4+ cd45ra+ t cells were isolated by a combination of positive and negative selection. putative cd4+ cd45ra+ cells expressed cd45ra (98.9%) and contained < 0.1% cd4+ cd45ro+ and < 0.5% cd4+ cd45ra+ cd45ro+ cells. putative cd45ro+ cells expressed cd45ro (90%) and contained 9% cd45ra+ cd45ro+ and < 0.1% cd4+ cd45ra+ cells. the responder frequency of dermatophagoides pteronyss ... | 1997 | 9301520 |
| biological activity of recombinant der p 2, der p 5 and der p 7 allergens of the house-dust mite dermatophagoides pteronyssinus. | der p 2, der p 5 and der p 7 are three allergens of the house-dust mite dermatophagoides pteronyssinus that have been cloned and expressed in escherichia coli as fusion proteins with glutathione-s-transferase (gst). we showed that these recombinant allergens produced immediate hypersensitivity skin-test reactions in 70, 60 and 52% respectively of a group of mite-sensitive allergic patients who were strongly positive to whole mite extract (wme). comparable positivities were found for serum levels ... | 1997 | 9303332 |
| potentiation of histamine release against inhalant allergens (dermatophagoides pteronyssinus) with bacterial antigens in bronchial asthma. | in the etiopathogenesis of bronchial asthma, the important role of bacterial infection is more evident every day, favoring inflammation and obstruction, that is, triggering an asthmatic response. we gathered 36 patients diagnosed of bronchial asthma with sensitization to dermatophagoides pteronyssinus and 32 healthy subjects. histamine release tests against staphylococcus aureus extract alone or together with d. pteronyssinus were performed, and the results were contrasted with or without the pr ... | 1997 | 9330183 |
| ige antibodies to aeroallergens in allergic children in são paulo, brazil. | the sensitization to inhaled allergens was studied in 80 brazilian children and 12 controls, aged 6 to 16 years. we performed skin tests with alternaria alternata, cat, dog, lolium perenne, grasses and the following domestic mites: blomia tropicalis, dermatophagoides pteronyssinus, dermatophagoides farinae, lepidoglyphus destructor, chortoglyphus arcutus and aleuroglyphus ovatus. the frequencies of positive skin tests (mean diameter > or = 23 mm) were respectively 15%, 11%, 11%, 6%, 7%, 95%, 92% ... | 1997 | 9330189 |
| lack of lysozyme activity of natural and yeast-derived recombinant der p 2. | for dermatophagoides pteronyssinus a number of allergens have been reported. although the function of several allergens is known, that of the house dust mite allergen der p 2 is unknown. a role as lysozyme has been suggested. we tested a yeast-derived recombinant der p 2 for its lysozymatic activity. this recombinant allergen and affinity-purified natural der p 2 lacked this enzymatic activity. whole-body mite extract retained its lysozymatic activity after removal of the group 2 allergen by mon ... | 1997 | 9338615 |
| salmeterol inhibits the allergen-induced mononuclear cell proliferation and downregulates gm-csf release and hla-dr expression by monocytes. | beta 2-adrenoceptor agonists are known to attenuate several functions of human mononuclear cells in response to activation. since monocytes and lymphocytes play a major pathogenetic role in allergic asthma, this study was designed to evaluate the hypothesis that salmeterol, a beta 2-adrenoceptor agonist with a long duration of action, could inhibit in vitro the allergen-induced activation of blood mononuclear cells (bmcs). bmcs were collected from subjects sensitized to dermatophagoides pteronys ... | 1997 | 9344832 |
| occupational allergic disease in cereal workers by stored grain pests. | it is well known that workers occupationally exposed to grain dust have a high prevalence of respiratory symptoms, but their pathogenesis remains obscure when sensitization to cereal flour cannot be demonstrated. storage mites, tenebroids, and cockroaches are stored-grain pests found in grain and cereal products frequently in our area, where the cereal industry is the most important industry. an epidemiological analysis of sensitization of these stored-grain pests was performed on 4379 patients ... | 1997 | 9350153 |
| gm-csf increases the ability of cultured macrophages to support autologous cd4+ t-cell proliferation in response to dermatophagoides pteronyssinus and ppd antigen. | previous studies have demonstrated an infiltration of monocytes and increased levels of granulocyte-macrophage colony-stimulating factor (gm-csf) in the asthmatic lung. to study the possible effects of this cytokine upon the differentiation and function of these newly recruited monocytes, we have developed a model in which monocytes isolated from human peripheral blood were differentiated into macrophages in serum in the presence or absence of gm-csf. after 7 days, the macrophages increased in s ... | 1997 | 9370934 |
| comparison of mrast and cap with skin end point titration for alternaria tenuis and dermatophagoides pteronyssinus. | there has been a recent explosion of new in vitro tests for the diagnosis of allergies. at present there is no general agreement on which type of in vitro test is best. recently our hospital switched in vitro testing from the modified radioallergosorbent system (mrast) to the pharmacia cap system (cap). while changing in vitro testing techniques, 47 patients were tested with both the mrast and cap tests. comparisons were made between the mrast and cap results of alternaria tenuis and dermatophag ... | 1997 | 9374169 |
| analysis of total ige and allergen-specific ige antibody levels of allergic children in taiwan. | with advances in technology, several in vitro screening tests such as mast and cap system have been used for analyzing the allergens involved in allergic diseases such as bronchial asthma (ba), allergic rhinitis (ar), atopic dermatitis (ad) and urticaria. in this study, cap system (pharmacia, sweden) was used to screen the prevalence of allergens responsible for these atopic diseases. a total of 392 children were enrolled in this study retrospectively, all these atopic children visited the aller ... | 1997 | 9401182 |
| ige responses to dermatophagoides pteronyssinus native major allergens der p 1 and der p 2 during long-term specific immunotherapy. | we investigated by elisa the ige response to whole extract of the house-dust mite dermatophagoides pteronyssinus (dp) and to the native major allergens, der p 1 and der p 2, in sera from 18 adult patients (group a) with dp-allergic asthma before (t0) and 1, 2, 3, and 4 (t1-t4) years after subcutaneous specific immunotherapy (sit). a qualitative reduction (p = 0.05) of the ige responses to dp and der p 2 was observed from t1 to t4, but a highly statistical significant decrease appeared at t3 (p < ... | 1997 | 9404565 |
| the effect of frequent vacuum cleaning on the house dust mite allergen, der p 1 in carpets: a pilot study. | to determine the effect of frequent vacuum cleaning of carpets on der p 1, the major group one allergen of the house dust mite dermatophagoides pteronyssinus. | 1997 | 9418840 |
| use of thiocyanate elution to estimate relative avidity of allergen specific ige antibodies. | although several methods for estimating avidity of antigen-antibody reactions are available, most are impractical for the study of human ige antibodies because of a requirement for pure allergen and antibody in relatively large amounts. to determine the relative avidity of specific ige antibodies for dermatophagoides pteronyssinus allergens, seven concentrations of the chaotropic thiocyanate ion were used to disrupt epitope-antibody binding in a specific ige immunoassay system, using sera from 1 ... | 1997 | 9429669 |
| evaluation of the mast cla allergy system for diagnosis of allergies to house dust mites and cats. | the mast cla system was evaluated against skin prick test (spt) for diagnosis of allergies to house dust mites (dermatophagoides pteronyssinus, dermatophagoides farinae) and cats. forty three asthmatic children were examined by spt and mast cla. chi-square analysis indicated significant association between spt and mast cla results for the house dust mites but not for cats. the sensitivities of mast cla for house dust mites and cats were 100 and 25% respectively; specificities were all less than ... | 1997 | 9438543 |
| kinetics of allergen expression in cultures of house dust mites, dermatophagoides pteronyssinus and d. farinae (acari: pyroglyphidae). | laboratory cultures of house dust mites dermatophagoides pteronyssinus (trouessart, 1897) and dermatophagoides farinae hughes, 1961 were used to study the population dynamics of the mites and the kinetics of antigen appearance. the analysis of extracts obtained after different incubation periods, carried out by sds-page, immunoblotting, and enzyme-linked immunosorbent assay, allows for the definition of 3 different growth phases: the latency phase (f1); the exponential growth phase (f2) during w ... | 1997 | 9439123 |
| comparison of major aeroallergens in taipei and kin-men. | ragweed pollen is an important aeroallergen in the usa, however, its role in the pathogenesis of allergic airway diseases in taiwan has not been reported. recently, a dramatic increase in patients with allergic rhinitis was noted in kin-men. most patients had characteristic seasonal variation with the symptoms waxing in august and waning in november, which correlated with the pollen season of ragweed. the aim of this study was to clarify the role of ragweed pollen in the pathogenesis of allergic ... | 1997 | 9444919 |
| sensitization to blomia tropicalis among asthmatic patients in taiwan. | house dust mites have been reported as one of the most important allergens in taiwan especially in asthmatic patients. this study was conducted to determine the allergenicity of blomia tropicalis and sensitization of asthmatic patients in taiwan. serial dust samples were collected every month between july 1993 and june 1994 from 13 houses of mite-allergic patients. about 1 m2 surface area of a quilt was vacuumed. the floating method was used to collect mites, then identification and counting wer ... | 1998 | 9482703 |
| indoor allergens and dwelling characteristics in two cities in spain. | indoor allergens are common causes of sensitization and asthma. climatic and home conditions can modify their levels. | 1997 | 9491197 |
| the epitope stability of group 1 and group 2 allergens in mite extracts. | commercial mite allergenic extracts sold in the us are prepared with whole body mites in buffer solutions containing 50% glycerol. mite extracts were reported to contain large number of proteolytic enzymes and their structural integrity in aqueous solutions have not been evaluated. | 1998 | 9494451 |
| allergen-induced migration of human cells in allergic severe combined immunodeficiency mice. | recently, we have shown that severe combined immunodeficiency (scid) mice, intraperitoneally reconstituted with peripheral blood mononuclear cells (pbmc) from dermatophagoides pteronyssinus (dpt)-sensitive patients, produced human ige and developed a pulmonary inflammatory-type reaction after exposure to allergen aerosol. in order to understand the potential mechanisms involved in the human cell migration in scid mice, we analysed their phenotypic profile in the lungs, spleen and thymus, 2 month ... | 1998 | 9496684 |
| the house dust mite allergen, dermatophagoides pteronyssinus, promotes type 2 responses by modulating the balance between il-4 and ifn-gamma. | a common property of allergens is their potential to generate type 2 cytokine responses. to understand the mechanisms involved in this phenomenon, we have evaluated the polarizing potential of a major allergen, dermatophagoides pteronyssinus 1 (der p 1), in an heterologous immunization system using the glutathione s-transferase of the parasite schistosoma mansoni (sm28-gst) as immunogen. in previous studies, we showed that immunization with the sm28-gst emulsified in cfa induced a nonpolarized i ... | 1998 | 9498790 |
| soluble cd30, dehydroepiandrosterone sulfate and dehydroepiandrosterone in atopic and non atopic children. | elevated levels of soluble cd30-scd30 (possibly related to th2) are seen in atopy and other diseases. dehydroepiandrosterone (dhea) may regulate th1/th2 balance. the aim was to study the relationship between the levels of scd30, dheas and dhea in atopic (a) and non atopic (b) children, to assess immune-endocrine interactions. we studied 36 children (8-15 years), 18 (a) monosensitised to dermatophagoides pteronyssinus (dpt.), and 18 (b) with isolate rhinitis (r) or asthma (ba) and r (33.3%) witho ... | 1998 | 9503096 |
| [the incidence of peculiar ige in response to inhalant allergens in relation to the age of the patient]. | 208 patients between the age of 1 month to 56 years, with symptoms of respiratory tract allergy were investigated. in all cases serum titre of sige to birch, thymoty, rye, mugwort pollen, alternaria alternata, dog, cat fur, dermatophagoides pteronyssinus and farinae was determined with the use of quidel immunoenzymatic method. we have found sige to alternaria alternata in 8 (38%), birch pollen in 5 (23.8%) dog fur in 5 (23.8%) and thymoty and rye pollen in 3 (14.3%) even among one years-old infa ... | 1997 | 9518328 |
| sudden infant death syndrome: a search for allergen hypersensitivity. | sudden infant death syndrome (sids) remains a diagnosis by exclusion which leaves few if any pathologic clues to its etiology. previous evaluations for anaphylaxis in sids have been few and limited. | 1998 | 9532970 |
| prevalence of atopy in students from málaga, spain. | epidemiologic studies are necessary to determine the prevalence of allergic diseases. this varies widely depending on allergen preparations and patients studied. | 1998 | 9532972 |
| determinants of eosinophil cationic protein in nasal lavages in children. | nasal lavages are increasingly used to assess airways inflammation in children. however, there are no studies assessing how measurement error as well as biological influences contribute to the concentration of nasal inflammatory parameters in a population based survey. | 1998 | 9543079 |
| sequencing and high level expression in escherichia coli of the tropomyosin allergen (der p 10) from dermatophagoides pteronyssinus. | the cdna encoding an allergen from the dust mite dermatophagoides pteronyssinus has been cloned and sequenced. the allergen (der p 10) is a tropomyosin that shared more than 65% identical residues with other invertebrate tropomyosins. the final recovery of recombinant der p 10 from the culture media after a single purification step was as much as 26 mg/l. the recombinant allergen is reactive to shrimp antitropomyosin igg antibodies and has a 5.6% frequency of ige reactivity in sera from mite-all ... | 1998 | 9545522 |
| immunoblotting analysis of twin sera provides evidence for limited genetic control of specific ige to house dust mite allergens. | although some studies have shown genetic control of specific ige responses to purified grass allergens, studies with other allergens have not supported this. the extent of such control for house dust mite (hdm) (dermatophagoides pteronyssinus) allergens is unclear. | 1998 | 9564802 |
| prevalence of skin reactions to aeroallergens in asthmatics of puerto rico. | in puerto rico, although a high prevalence of asthma has been reported, the sensitization rates to aeroallergens in these patients is unknown. the purpose of this study using a case control design, was to determine and compare the rates of sensitization to common aeroallergens in an asthmatic population of 576 asthmatics and 144 healthy controls. a skin prick test was conducted using standardized extracts of dermatophagoides farinae (df) and d. pteronyssinus (dp), house dust (hd), cat hair and e ... | 1997 | 9580406 |
| identification of tyrophagus putrescentiae allergens and evaluation of cross-reactivity with dermatophagoides pteronyssinus. | house dust mites are the most common cause of allergic sensitization in respiratory allergic patients in the world. tyrophagus putrescentiae (tp), which was followed by dermatophagoides farinae (df) and dermatophagoides pteronyssinus (dp), has been reported as the third most common house mite in korea. we previously reported that many respiratory allergic patients had become concomitantly sensitized to dp, df and tp. the aims of this study were to identify the allergic components of tp and to ev ... | 1998 | 9587250 |
| acute myelitis with hyperigeaemia and mite antigen specific ige: atopic myelitis. | an occurrence of acute localised myelitis was recently seen in four adult patients with atopic dermatitis who had hyperigeaemia and mite antigen specific ige. the total and mite antigen specific ige was therefore studied in serum samples from 19 consecutive patients with acute localised myelitis of unknown aetiology, 56 patients with clinically definite multiple sclerosis, and 40 healthy controls. the total ige concentration was significantly higher in acute localised myelitis (median=360 u/ml) ... | 1998 | 9598690 |
| prevalence and risk factors of latex sensitization in an unselected pediatric population. | the prevalence of latex sensitization has been investigated in population groups considered at high risk, but it has not been systematically surveyed among the general population. | 1998 | 9600498 |
| multiple sclerosis with mite antigen-specific ige. | the total and mite antigen-specific ige in the sera were measured in 60 patients with multiple sclerosis (ms) and 40 healthy controls by an enzyme-linked immunosorbent assay using two common mite antigens, dermatophagoides farinae and dermatophagoides pteronyssinus, in order to study the role of atopy in ms. neither the total ige level nor the frequency of mite antigen-specific ige differed significantly between the ms patients (mean total ige 162 u/ml, 42% mite ige-positive) and healthy control ... | 1998 | 9619635 |
| indoor allergens and longitudinal fev1 decline in older adults: the normative aging study. | we investigated the relationship between home allergen exposure and decline in fev1 in 10 asthmatic and 30 randomly selected, age-matched, nonasthmatic participants in the normative aging study. we defined asthma as subject-reported wheezing apart from colds, with either a physician's diagnosis of asthma or a methacholine pd20 fev1 of 8.6 micromol or less. we examined the relationship between the annual decline in fev1 and the concentrations of the cockroach (blattella germanica) allergens bla g ... | 1998 | 9648697 |
| increased expression of high affinity ige (fcepsilonri) receptor-alpha chain mrna and protein-bearing eosinophils in human allergen-induced atopic asthma. | fcepsilonri receptors play an important role in allergen-induced mediator release and antigen presentation by mast cells, basophils, and monocyte/macrophages in atopic disorders. the expression of fcepsilonri by tissue eosinophils in atopic asthma after allergen challenge has not been established. for this reason we attempted to identify mrna and protein product + fcepsilonrialpha eosinophils in cytospins made from bronchoalveolar lavage (bal) from atopic asthmatics (n = 9) and nonatopic normal ... | 1998 | 9655735 |
| salmeterol enhances the inhibitory activity of dexamethasone on allergen-induced blood mononuclear cell activation. | the possibility that salmeterol could interfere with the inhibitory effect of glucocorticosteroids on allergen-induced activation of leukocytes was assessed in cultures of human blood mononuclear cells (bmcs). bmcs, recovered from 27 patients (21 +/- 4 years of age) sensitized to dermatophagoides pteronyssinus (der p), were incubated with a purified der p extract (10 micrograms/ml) for 7 days in the presence of salmeterol (10(-8) and 10(-7) m) and/or dexamethasone (10(-10) and 10(-9) m). bmc pro ... | 1998 | 9670302 |
| a comparative study of the tryptase release test and the cellular allergen stimulation test (cast) in mite sensitive patients. | the stimulation of blood basophils to release mediators in vitro is widely used for diagnosis of allergic diseases. tryptase release and sulphidopeptideleukotriene production are both triggered by cross-bridging of adjacent ige molecules on the surface of ige-bearing basophils. | 1998 | 9677141 |
| requirement of cd28-cd86 costimulation for allergen-specific t cell proliferation and cytokine expression. | allergen-specific t lymphocytes biased to the production of type 2 cytokines play an important role in the pathophysiology of atopic disease. it is not known whether optimal activation of these t cells requires costimulation via interaction of b7 (cd86/cd80) with cd28. | 1998 | 9720814 |
| reactivity to recombinant house-dust-mite allergens in asthma and rhinitis in a tropical environment. | house-dust mites contain components that are allergenic in mite-sensitive patients, and a number of these have been produced in recombinant form. | 1998 | 9722232 |
| [factors aggravating bronchial asthma in urban children (i)--the involvement of indoor air pollution]. | the aggravation of bronchial asthma in today's urban child population was studied by an epidemiological study in order to elucidate the involvement of indoor air pollution in relation to housing style. the asthma group consisted of 210 children under 12 years old who had been recently diagnosed as having bronchial asthma and under the care of osaka prefectural habikino hospital. the non-asthma group consisted of 180 children under 12 years old who had been under care at osaka prefectural hospita ... | 1998 | 9739594 |
| [factors aggravating bronchial asthma in urban children (ii)--the involvement of atopy and serum fatty acids, and their interaction with urban living environment]. | the aggravation of bronchial asthma in today's urban child population was studied by an epidemiological study in order to elucidate the involvement of food habits as well as individual factors such as age, sex, and history of atopic dermatitis, and the interaction with urban living environments. the asthma group consisted of 202 children under 12 years old who had been recently diagnosed as having bronchial asthma and under the care of osaka prefectural habikino hospital. the non-asthma group co ... | 1998 | 9739595 |
| variability of house dust mite allergen exposure in dwellings. | the variability of repeated house dust mite (hdm) allergen determinations at the same site within 3-24 months was evaluated on previously collected samples. between two and four repeated measurements of der p 1, a major allergen of dermatophagoides pteronyssinus and der f 1, a major allergen of d. farinae, on 46 carpets and 31 mattresses were analyzed. in 90% of carpets and mattresses, hdm allergen concentrations were clinically relevant (at least one measurement >0.1 microg der p 1 + der f 1/g ... | 1998 | 9755142 |
| prevalence of sensitization to aeroallergens in california patients with respiratory allergy. allergy skin test project team. | the number of allergy skin tests required to evaluate patients with respiratory allergy has recently been challenged by the managed care community. | 1998 | 9759795 |
| association between giardiasis and allergy. | previous studies have indicated that there may be an association between infection by the intestinal protozoan giardia lamblia and the expression of allergic disease. | 1998 | 9759805 |
| ascaris-specific ige and allergic sensitization in a cohort of school children in the former east germany. | helminthic infections induce an il-4-dependent polyclonal stimulation of ige synthetization. it is still unclear, however, what role helminths play in allergic sensitization. | 1998 | 9768582 |
| genetic regulation of dermatophagoides pteronyssinus-specific ige responsiveness: a genome-wide multipoint linkage analysis in families recruited through 2 asthmatic sibs. collaborative study on the genetics of asthma (csga). | dermatophagoides pteronyssinus (der p) is one of the most frequently implicated allergens in atopic diseases. although hla could play an important role in the development of the ige response to the der p allergens, genetic regulation by non-hla genes influences certain hla-associated ige responses to complex allergens. | 1998 | 9768585 |
| linkage analysis of dermatophagoides pteronyssinus-specific ige responsiveness with polymorphic markers on chromosome 6p21 (hla-d region) in caucasian families by the transmission/disequilibrium test. collaborative study on the genetics of asthma (csga). | recently, we have obtained evidence for linkage between der p 1-specific ige antibodies and markers on chromosome 6p21 (hla-d region) in a genome-wide screening in caucasian families recruited as a part of the collaborative study on the genetics of asthma (csga). | 1998 | 9768586 |
| genetic influences of chromosomes 5q31-q33 and 11q13 on specific ige responsiveness to common inhaled allergens among african american families. collaborative study on the genetics of asthma (csga). | we have recently conducted a genome-wide screening for genes influencing dermatophagoides pteronyssinus-specific ige responsiveness as a part of the collaborative study on the genetics of asthma (csga), which showed evidence for linkage in some regions, including chromosomes 5131-q33 and 11q13 in african american families. | 1998 | 9768587 |
| hypersensitivity to chironomid larvae. | chironomid larvae (red midge larvae) are often used by aquarists as fish food. their hemoglobins can cause ige-mediated allergic diseases in exposed and unexposed people. the aim of this study was to find out the prevalence of positive skin tests to chironomids in patients suffering from rhinitis and/or bronchial asthma in ciudad real (spain). a total of 465 patients were submitted to skin prick tests with chironomids in addition to common inhalant allergens. the patients with positive skin pric ... | 1998 | 9777536 |
| sensitization to cockroach allergens in a sample from the urban population living in naples (southern italy). | the aim of this study was to evaluate the prevalence of allergic sensitization to cockroach allergens in a group of atopic patients living in the urban area of naples. four hundred and fourteen consecutive patients were submitted to the following diagnostic procedures: anamnestic data; clinical examination; and skin prick test by using a standardized pricker and allergenic extracts, one of which contained a mix of whole bodies of blattella orientatis, blattella germanica and periplaneta american ... | 1998 | 9777540 |
| indoor factors associated with concentrations of house dust mite allergen, der p 1, in a random sample of houses in norwich, uk. | studies of house dust mite allergen concentrations suggest large variations both within and between homes. it is likely that different household characteristics influence allergen levels in different locations within the house. | 1998 | 9824386 |
| expression of b7 co-stimulatory molecules and cd1a antigen by alveolar macrophages in allergic bronchial asthma. | lung allergen recognition that takes place in the airways of asthmatic subjects is still a controversial matter. | 1998 | 9824408 |
| oral tolerance induced to house dust mite extract in naive and sensitized mice: evaluation of immunoglobulin g anti-immunoglobulin e autoantibodies and igg-ige complexes. | we investigated the effect on specific antibody response of naive and sensitized mice orally administrated with low (0.25 mg) or high (10.0 mg) doses of dermatophagoides pteronyssinus (dp) extract. we also examined the effect of oral administration of dp on the production of autoantibodies to immunoglobulin g (igg) and immunoglobulin e (ige). naive and sensitized mice both showed a marked down-regulation of ige antibody production, regardless of the dose of dp. we also detected an inhibitory eff ... | 1998 | 9824475 |
| sensitization to mite allergens and acute asthma in a tropical environment. | the objective of this study was to determine whether sensitization to certain aeroallergens is associated with acute asthma in cartagena, colombia. ninety-nine subjects with acute asthma and 100 controls evaluated for other reasons were studied at a local emergency room. sensitization was established by specific ige determinations. statistically significant differences between asthmatic subjects and controls were found in the prevalence of specific ige to dermatophagoides pteronyssinus, 64.6% vs ... | 1998 | 9827423 |
| water balance of dermatophagoides pteronyssinus (acari: pyroglyphidae) maintained by brief daily spells of elevated air humidity. | a facility was constructed to expose cultured dermatophagoides pteronyssinus (trouessart) to 24-h cycles of changing relative humidity; low humidity was interrupted every day by a period of high humidity. the temperature was kept at 16 degrees c, a common temperature on the ground floor of dutch houses. partially dehydrated mites were exposed to various humidity regimes in the absence of food and liquid water. some mites gained weight when moist air was given for only 1.5 h every day. in the pre ... | 1998 | 9835678 |
| role of t-helper type 2 cytokines in down-modulation of fas mrna and receptor on the surface of activated cd4(+) t cells: molecular basis for the persistence of the allergic immune response. | the mechanisms responsible for persistence of t lymphocytes at the sites of allergic inflammation are not completely understood. activated t cells, usually expressing fas on their surface, undergo activation-induced apoptotic death, thus limiting the dangerous consequences of a persistent immune reaction. we have previously shown that pulmonary t lymphocytes from untreated asthmatic subjects do not express surface fas receptors nor do they contain fas mrna, yet they display normal levels of fas ... | 1998 | 9837865 |
| the allergenic acarofauna of house dust from dwellings, hospitals, libraries and institutes in upper silesia (poland). | a survey of dust samples from dwellings, hospitals and some public utilities (libraries, institutes) in several upper silesian towns was undertaken to determine the prevalence, number and species of mites. total seasonal dynamics of dust mite species of the family pyroglyphidae in dust from beds, floors/carpets and upholstery furniture in dwellings was analysed. over a 4-years period, 402 dust samples were studied: 238 samples from dwellings, 122 samples from hospitals, 14 from libraries and 28 ... | 1998 | 9852494 |
| [european study of asthma. prevalence of atopy in young adults of 5 areas in spain. spanish group of european asthma study]. | the aim of the current study is to show the prevalence of atopy in five spanish areas, and its variability according to area, age and gender. | 1998 | 9859090 |
| b7 costimulation is required for il-5 and il-13 secretion by bronchial biopsy tissue of atopic asthmatic subjects in response to allergen stimulation. | asthma is a complex disorder characterized by airway hyperreactivity and inflammation. to analyze cellular interactions required for the secretion of cytokines by the bronchial mucosa, we have evaluated the ex vivo response of tissue explants to allergen. endobronchial mucosal biopsy tissue from mild atopic asthmatic subjects and normal control subjects were maintained in culture for 24 h. to detect reactivity to allergen, the explants were stimulated with dust mite extract dermatophagoides pter ... | 1999 | 9870929 |
| allergen-specific ige and iggd antibodies in atopic and normal dogs. | intradermal skin tests (idsts) were performed on 65 atopic and 24 normal dogs. the levels of allergen-specific ige and iggd antibodies were determined in serum samples by enzyme-linked immunosorbent assay (elisa) using the same 12 allergens that were used in the idst on normal dogs. the correlation between the levels of ige and iggd to dermatophagoides farinae (df) and dermatophagoides pteronyssinus (dp) was examined. the sensitivity, specificity and positive and negative predictive values of al ... | 1998 | 9880099 |
| molecular analysis of the group 1 and 2 allergens from the house dust mite, euroglyphus maynei. | there is increasing evidence that the house dust mite euroglyphus maynei may be a significant source of allergic sensitization. the structural information for the e. maynei allergens is largely restricted to a single partial genomic sequence of eur m 1. | 1999 | 9925958 |
| the effect of dry heat on mite, cat, and dog allergens. | various techniques have been tried in an attempt to reduce allergen levels in homes. this study investigated the effect of dry heat on mite, cat, and dog allergens. | 1998 | 9930600 |
| [diagnostic use of enzymatic rast skin tests and determination of eosinophils in nasal mucosa in allergic rhinitis]. | allergic rhinitis is the most frequent disease mediated by immunoglobulin e (ige). nasal challenge is the gold standard for the diagnosis of allergic rhinitis. skin tests (st) are the most used diagnostic method to detect the presence of specific ige bind to skin mast cells. the exposition of the nasal mucous membrane to the allergen is followed by an increase of the local eosinophils; the count of eosinophils in nasal mucous (enm) is a diagnostic test for allergic rhinitis. enzymatic rast or en ... | 1998 | 9951327 |
| evaluating the relevance of aeroallergen sensitization in atopic eczema with the atopy patch test: a randomized, double-blind multicenter study. atopy patch test study group. | ige-mediated sensitization of questionable clinical relevance is routinely demonstrated in patients with atopic eczema (ae) by skin prick test or radioallergosorbent test (rast). we report the results of a randomized, double-blind multicenter study in patients with ae on the relevance and technical aspects of aeroallergen patch testing (atopy patch test, apt). | 1999 | 10025743 |
| immunogenetics of atopic asthma: association of drb1*1101 dqa1*0501 dqb1*0301 haplotype with dermatophagoides spp.-sensitive asthma in a sample of the venezuelan population. | genes linked to the major histocompatibility complex (mhc), have been implicated in atopic asthma. asthma is highly prevalent in the venezuelan population (estimated at 20%) and genetic markers are needed to identify populations at risk and plan intervention strategies. | 1999 | 10051703 |
| the kinetics of change in cytokine production by cd4 t cells during conventional allergen immunotherapy. | the effect of conventional allergen immunotherapy on allergen-specific t lymphocyte cytokine production is incompletely understood, particularly during the initial phase of treatment. | 1999 | 10069882 |