| anisakis simplex: the high prevalence in madrid (spain) and its relation with fish consumption. | anisakiosis is a nematodosis with high prevalence in spain. in this work we (a) investigated whether a recently introduced elisa of anisakis simplex-specific ige in serum suffers from cross-reactivity with other common allergens; (b) used this assay to obtain an estimate of the prevalence of a. simplex-specific ige in the population of madrid; and (c) related positivity to fish consumption habits. no evidence of cross-reactivity between the elisa and other allergens was found. the prevalence of ... | 2008 | 17825294 |
| parasite communities of common dolphins (delphinus delphis) from patagonia: the relation with host distribution and diet and comparison with sympatric hosts. | we studied the helminths of 18 common dolphins, delphinus delphis, from northern patagonia. parasites were found only in the gastrointestinal tract. four species were in the stomach, the nematode anisakis simplex, the acanthocephalan corynosoma cetaceum, and the digeneans braunina cordiformis and pholeter gastrophilus, plus 1 digenean in the hepatopancreatic ducts, oschmarinella rochebruni. infection levels were low (0-155 parasites). braunina cordiformis and c. cetaceum were the most prevalent ... | 2007 | 18163339 |
| [use of anamnesis and immunological techniques in the diagnosis of anisakidosis in patients with acute abdomen]. | anisakis simplex can be a cause of digestive symptoms. our aim was to evaluate the epidemiological antecedents and immunological data available for a differentiation between patients with anisakidosis and those with other acute abdominal problems. | 2008 | 18416639 |
| identification and allergenic characterisation of a new isoform of the a. simplex allergen ani s 4. | anisakis simplex hypersensitivity is a growing disease in developed countries. a positive diagnosis usually leads to the dietary recommendation of avoiding fish and seafood consumption. the protein ani s 4 is a clinically relevant allergen due to its heat and pepsin resistant properties and its importance in the anaphylaxis process. the attempt of cloning ani s 4 has led to the identification and characterisation of a new isoform that differs only in one amino acid with the previously published. ... | 2008 | 18501441 |
| parasites in stranded cetaceans of patagonia. | there is an increasing interest in parasites of marine mammals of argentina. here, we examined several poorly known cetaceans, i.e., 2 spectacled porpoises and 1 burmeister's porpoise (phocoenidae), and 1 gray's beaked whale and 1 cuvier's beaked whale (ziphidae); we also updated the parasite information for 1 sperm whale (physeteridae). these hosts strand only occasionally. we found anisakis simplex s.l. in 2 spectacled porpoises and the burmeister's porpoise, and recorded its distribution amon ... | 2008 | 18576713 |
| protein contact dermatitis and allergic asthma caused by anisakis simplex. | | 2009 | 19338604 |
| [acute abdomen by anisakiasis and globalization]. | the aa report a case of a patient with acute abdomen caused by intestinal anisakiasis, a fish-transmitted infection that results from ingestion of raw or improperly cooked fish contaminated by live larvae of anisakis simplex, a round worm from the order of ascaridida. | 2009 | 19537127 |
| anisakid larvae in atlantic salmon (salmo salar l.) grilse and post-smolts: molecular identification and histopathology. | the molecular identification and histopathology are described for the parasitic larvae of a nematode species present in the abdominal cavity of atlantic salmon ( salmo salar ) grilse caught in fish traps on their natal river in the west of ireland and post-smolts collected during experimental trawls on the continental shelf edge of the northeast atlantic ocean. larvae in the adult and juvenile salmon were identified as anisakis simplex sensu stricto by pcr amplification and rflp and sequencing o ... | 2010 | 19747018 |
| pathological and ultrastructural studies on anisakis simplex rudolphi-1809 infecting carangoides bajad with special reference to intestinal maturation in puppies. | thirty five (70%) of 51 carangoides bajad were naturally infected with anisakis simplex during the period from september 2007 to january 2008. the fish were collected from eastern south coast of the red sea at hurgada. the morphological and ultrastructures of anisakis larvae and adults, and the induced lesions in the fish (intermediate host), five puppies (final host) were orally given infected fish. the body of the larvae is gradually tapering towards the anterior part. it is covered by striate ... | 2009 | 19795767 |
| different clinical presentation of anisakis simplex associated urticaria is dependent on the frequency of raw fish intake. | | 2010 | 20089345 |
| anisakiasis, an underestimated infection: effect on intestinal permeability of anisakis simplex-sensitized patients. | anisakis simplex is a parasite that, if present in uncooked and contaminated saltwater fish, can invade the human gut. two different clinical situations are recognized: the first, known as a gastrointestinal disease, varying from an asymptomatic episode to vomiting and diarrhea, and the second, classified as an adverse reaction to food, characterized by a wide spectrum of allergic reactions like rhinitis, conjunctivitis, or even anaphylaxis causing hypotension and/or shock. the intestinal epithe ... | 2010 | 20367330 |
| parasite excretory-secretory proteins elicit trif dependent cxcl1 and il-6 mediated allergic inflammation. | currently, little information is available regarding innate immunity to helminthic parasite infection. in this study, we isolated the excretory-secretory (es) proteins from anisakis simplex (sea mammal intestinal parasite) third stage larva. we determined that the levels of il-17 in the lung and lung draining lymph node of mice were increased sixfold as a result of intranasal treatment with es proteins. the es protein treatment elicited pro-inflammatory cytokine and chemokine secretion (especial ... | 2010 | 20500664 |
| aggregation patterns of macroendoparasites in phylogenetically related fish hosts. | summary: macroparasites are generally aggregated within their hosts with infection and aggregation levels resulting from a continuous arms race between maintaining high mating probability and host mortality low for which host and environmentally related factors contribute to some extent. here, infection and aggregation patterns of the macroendoparasites infecting the flatfish citharus linguatula, arnoglossus laterna, lepidorhombus boscii, scophthalmus rhombus and platichthys flesus in 3 areas al ... | 2010 | 20500921 |
| larvicidal constituents of zingiber officinale (ginger) against anisakis simplex. | in this study, we investigated the anthelmintic activity of [10]-shogaol, [6]-shogaol, [10]-gingerol and [6]-gingerol, compounds isolated from the roots of zingiber officinale l., zingiberaceae (ginger), against anisakis simplex. the above compounds kill or reduce spontaneous movement in a. simplex larvae. the maximum lethal efficacy of [10]-shogaol and [10]-gingerol was approximately 80% and 100%, respectively. we further examined the time course of compound-induced loss of mobility in a. simpl ... | 2010 | 20533167 |
| molecular differentiation and phylogenetic relationships of three angiostrongylus species and angiostrongylus cantonensis geographical isolates based on a 66-kda protein gene of a. cantonensis (nematoda: angiostrongylidae). | the phylogenetic relationships and molecular differentiation of three species of angiostrongylid nematodes (angiostrongylus cantonensis, angiostrongylus costaricensis and angiostrongylus malaysiensis) were studied using the ac primers for a 66-kda protein gene of a. cantonensis. the ac primers successfully amplified the genomic dna of these angiostrongylid nematodes. no amplification was detected for the dna of ascaris lumbricoides, ascaris suum, anisakis simplex, gnathostoma spinigerum, toxocar ... | 2010 | 20566366 |
| inhibition of cytokine production in gastro-allergic anisakiasis and anisakis simplex sensitization-associated chronic urticaria. | | 2010 | 20591123 |
| anisakis simplex sensitization-associated urticaria: short-lived immediate type or prolonged acute urticaria. | acute urticaria is defined as evanescent wheals with a duration period of up to 6 weeks. yet within acute urticaria, ige-mediated urticaria lasts rarely more than 48 h, whereas longer duration periods are frequently unfruitful with respect to diagnostic work-up. we hypothesize the differences in immunologic features in immediate type urticaria versus prolonged acute urticaria within the model of anisakis simplex (a. simplex) sensitization-associated urticaria. we included 57 patients with gastro ... | 2010 | 20628748 |
| elucidation of ige-binding epitopes of ani s 1: the major anisakis simplex allergen. | ani s 1, the major allergen of anisakis simplex, is expected to be a useful antigen in allergen-specific immunotherapy of anisakis allergy. to avoid side-effects such as anaphylactic shock in immunotherapy, it is desirable to use not native allergens but hypoallergenic mutants devoid of ige-binding epitopes. this study was hence aimed to elucidate ige-binding epitopes of ani s 1 toward the development of hypoallergenic ani s 1 mutants. mapping experiments using 32 peptides (p1-32) revealed that ... | 2010 | 20655337 |
| [cloning and expression of l-like cysteine protease of anisakis simplex]. | to clone and express the full lenth of l-like cysteine protease gene of anisakis simplex (ascp). | 2010 | 20666320 |
| genetic variability of anisakis simplex s.s. parasitizing european hake (merluccius merluccius) in the little sole bank area in the northeast atlantic. | in this study, we researched the presence of anisakids in specimens of merluccius merluccius caught in the area of little sole bank, in the northeast atlantic, and found that 100% of the european hake examined were infected and showed high average values of abundance (976.88) and intensity (976.88). the larvae were identified in morphological terms as morphotype type i and in molecular terms as anisakis simplex s.s via polymerase chain reaction (pcr) restriction fragment length polymorphism of t ... | 2010 | 20694563 |
| the hidden sense of symptoms: urticaria can be beneficial. | evolutionary medicine can be useful when analysing the origin of disease and symptoms. acute urticaria or anaphylaxis is bothersome and potentially life-threatening. we analyse this symptom-complex in the context of gastro-allergic anisakiasis (gaa), where the human is an incidental host for the cosmopolitan fish-nematode anisakis simplex (a. simplex). the immunological response against this nematode resembles that against other helminths, but overt expression of allergic symptoms is by far more ... | 2010 | 20729003 |
| anisakidosis: perils of the deep. | anisakidosis, human infection with nematodes of the family anisakidae, is caused most commonly by anisakis simplex and pseudoterranova decipiens. acquired by the consumption of raw or undercooked marine fish or squid, anisakidosis occurs where such dietary customs are practiced, including japan, coastal regions of europe, and the united states. severe epigastric pain, resulting from larval invasion of the gastric mucosa, characterizes gastric anisakidosis; other syndromes are intestinal and ecto ... | 2010 | 20804423 |
| [detection of anisakid nematodes by an sybr green i real-time pcr]. | to establish an sybr green i real-time quantitative pcr method for the detection of anisakid nematodes with zoonotic potential from taiwan strait. | 2010 | 20806503 |
| molecular identification of anisakis type i larvae isolated from hairtail fish off the coasts of taiwan and japan. | anisakid nematodes are known to cause the zoonotic disease, anisakiasis, through the consumption of raw or undercooked fish. the parasites most frequently associated with the disease in humans are categorized as anisakis type i, which comprise several species of the genus anisakis. the larvae show primitive forms and lack the detailed morphological characteristics required for precise species identification. thus, molecular characterization is necessary for determining the species of anisakis ty ... | 2010 | 20826034 |
| chronic urticaria is associated with a differential helminth-arthropod-related atopy phenotype. | the relationship between atopic sensitization and chronic urticaria is still controversial. in this study, we aimed to compare the prevalence of aeroallergen sensitization in chronic urticaria patients with (cu/as+) and without (cu/as-) sensitization against anisakis simplex. forty-nine cu/as+ and 80 cu/as- patients were studied and skin prick tests (spt) were performed against aeroallergens. we assessed sensitization in a subgroup of patients with allergic rhinoconjunctivitis and/or bronchial a ... | 2010 | 20883361 |
| occurrence and identification of anisakis spp. (nematoda: anisakidae) isolated from chum salmon (oncorhynchus keta) in korea. | the prevalence of infection and the identification of anisakid larvae in chum salmon (oncorhynchus keta) from the namdae river, the east coast of korea, were investigated. in total, 8,358 larvae were collected from 120 fish samples (male = 58 fish, female = 62 fish) in 2008. fish samples were collected during october and november 2008. all the chum salmon samples (120/120, 100%) caught were infected with anisakid larvae with a high intensity (69.65 ± 48.58 larvae/host). they were mostly found in ... | 2010 | 20938685 |
| multiple acute parasitization by anisakis simplex. | hypersensitivity to anisakis is an increasingly prominent medical problem throughout the world, due to a better understanding of diseases induced by parasites and to modern culinary habits of eating raw or undercooked fish. we describe the case of a patient who presented epigastric pain, wheals, erythema, and pruritus 3 hours after the ingestion of fish. more than 200 larvae were obtained by endoscopy. however, the patient only developed an immune response with specific immunoglobulin e and eosi ... | 2010 | 20945613 |
| anisakiasis outbreak by anisakis simplex larvae associated to peruvian food in spain. | | 2010 | 21039074 |
| experimental challenge of anisakis simplex sensu stricto and anisakis pegreffii (nematoda: anisakidae) in rainbow trout and olive flounder. | the third-stage larvae of anisakis simplex sensu lato (s.l.) are found in many marine fishes. to ensure food safety, it is important to determine whether these larvae are present in the body muscle of commercial fish species. however, there is little information regarding the tissue specificity of anisakis and two of its sibling species, a. simplex sensu stricto (s.s.) and anisakis pegreffii, that are common in marine fish in japanese waters. we orally challenged rainbow trout (oncorhynchus myki ... | 2010 | 21122822 |
| [exposure to anisakis simplex in the fishing industry: an emerging aspect in occupational medicine?]. | | 2010 | 21141458 |
| [infection and physico-chemical characteristics of anisakis among marine fish caught in zhoushan fishery.] | objective: to study the rates of infection and physicochemical characteristics of the third stage anisakis simplex larvae among marine fish caught in zhoushan fishery. methods: fish were dissected to detect anisakis larvae and identified morphologically. the survival tolerance of the third stage anisakis simplex larvae in various medium, anthelmintic drug, temperature were studied in laboratory. results: the total infection rate of anisakis simplex larvae in fish was 49.10%. high rates of anisak ... | 2010 | 21162864 |
| amelioration of intestinal colitis by macrophage migration inhibitory factor isolated from intestinal parasites through toll-like receptor 2. | in a previous study, we cloned type ii mifs (as-mif) from anisakis simplex 3rd stage larva and expressed a recombinant protein that suppressed allergic airway inflammation via regulatory t (cd4(+) cd25(+) foxp3(+) t; t(reg) )-cell recruitment. in this study, in an effort to evaluate the function of ras-mif on another immune disease, we induced intestinal inflammation in mice using dextran sodium sulphate (dss) with or without the application of ras-mif treatment to the mice. as a consequence, we ... | 2011 | 21204854 |
| parasitism between anisakis simplex (nematoda: anisakidae) third-stage larvae and the spotted mackerel scomber australasicus with regard to the application of stock identification. | the nematode fauna of 369 spotted mackerel of the species scomber australasicus, collected off the northeastern taiwanese coast of the northwestern pacific, was investigated monthly from april 2004 to march 2005. the following nematode species were recorded: anisakis simplex complex, hysterothylacium aduncum, porrocaecum decipiens and raphidascaris trichiuri. the seasonal variation in the infection with a. simplex third stage larva (l3) was studied throughout the 12 months. the prevalence of a. ... | 2010 | 21211909 |
| molecular characterization and phylogeny of anisakid nematodes from cetaceans from southeastern atlantic coasts of usa, gulf of mexico, and caribbean sea. | in the present study, 407 anisakid nematodes, collected from 11 different species of cetaceans of the families delphinidae, kogiidae, physeteridae, and ziphiidae, from the southeastern atlantic coasts of usa, the gulf of mexico, and the caribbean sea, were examined morphologically and genetically characterized by pcr restriction fragment length polymorphism to identify them to species level, assess their relative frequencies in definitive hosts, and determine any host preference. sequence data f ... | 2011 | 21212982 |
| [a study of the infection and physicochemical characteristics of the marine fish infected by anisakis l₃ caught in zhoushan fishery]. | the aim of this research was to investigate the third phase anisakis simplex larvae (anisakis l₃) infection in marine fish caught in zhoushan fishery and to find out its physicochemical and biological characteristics. | 2010 | 21215135 |
| cytokine production and immune modulation by anisakis simplex. | | 2011 | 21226728 |
| anisakis simplex-induced anaphylaxis. | in recent years, anisakis simplex has been shown to be an important etiologic agent responsible for food allergy and for gastrointestinal anisakiasis. we report a 61-year-old woman presenting with generalized urticaria and subsequent anaphylaxis after ingestion of raw mackerel. she rapidly recovered with administration of epinephrine and endoscopic extraction of an a. simplex larva. serologic testing revealed specific ige antibody to a. simplex was positive whereas that to mackerel was negative. ... | 2011 | 21258954 |
| identification of novel three allergens from anisakis simplex by chemiluminescent immunoscreening of an expression cdna library. | anisakis simplex is a representative nematode parasitizing marine organisms, such as fish and squids, and causes not only anisakiasis but also ige-mediated allergy. although 10 kinds of proteins have so far been identified as a. simplex allergens, many unknown allergens are considered to still exist. in this study, a chemiluminescent immunoscreening method with higher sensitivity than the conventional method was developed and used to isolate ige-positive clones from an expression cdna library of ... | 2011 | 21262386 |
| ani s 10, a new anisakis simplex allergen: cloning and heterologous expression. | anisakiasis is a human disease caused by accidental ingestion of larval nematodes belonging to the anisakidae family. anisakiasis is often associated with a strong allergic response. diagnosis of a. simplex allergy is currently carried out by test based on the ige reactivity to a complete extract of l3 anisakis larvae although the specificity of these diagnostic tests is poor. improving the specificity of the diagnostic test is possible using purified recombinant allergens. a new anisakis allerg ... | 2011 | 21310242 |
| morphological and molecular characterization of anisakis larvae (nematoda: anisakidae) in beryx splendens from japanese waters. | the third-stage (l3) larvae of anisakis, which are the etiological agents of human anisakiasis, have been categorized morphologically into anisakis type i larvae and anisakis type ii larvae. genetic analysis has allowed easy identification of these larvae: anisakis type i larvae include the species anisakis simplex sensu stricto, anisakis pegreffii, anisakis simplex c, anisakis typica, anisakis ziphidarum, and anisakis nascettii, whereas anisakis type ii larvae include the species anisakis physe ... | 2011 | 21377540 |
| distribution of anisakis species larvae from fishes of the japanese waters. | human anisakiasis is caused by the consumption of raw, marinated or undercooked fish and squid infected with nematodes of the genus anisakis dujardin, 1845. in view of food safety, this study was carried out to examine the distribution of anisakis species in marine fishes within japanese waters. seven fish species from six localities were collected and examined for anisakis infection. morphological and molecular (its region and mtdna cox2 gene) characterization revealed the presence of two, amon ... | 2011 | 21397715 |
| gastrointestinal bleeding due to suspected anisakiasis: challenging differential diagnosis for a rare disease. | as compared with eastern countries, the incidence of gastrointestinal diseases caused by the ingestion of nematode eggs is very low in europe, in spite of the fact that the consumption of raw fish has increased in these countries as well in recent years. the authors present here, a rare case of gastrointestinal bleeding due to anisakis simplex. this is a very uncommon clinical presentation which rise several problems in diagnosis and management. | 2011 | 21445646 |
| anisakis simplex larvae: infection status in marine fish and cephalopods purchased from the cooperative fish market in busan, korea. | the infection status of marine fish and cephalopods with anisakis simplex third stage larva (l3) was studied over a period of 1 year. a total of 2,537 specimens, which consisted of 40 species of fish and 3 species of cephalopods, were purchased from the cooperative fish market in busan, korea, from august 2006 to july 2007. they were examined for a. simplex l3 from the whole body cavity, viscera, and muscles. a. simplex l3 were confirmed by light microscopy. the overall infection rate reached 34 ... | 2011 | 21461267 |
| miss-prot: web server for self/non-self discrimination of protein residue networks in parasites; theory and experiments in fasciola peptides and anisakis allergens. | infections caused by human parasites (hps) affect the poorest 500 million people worldwide but chemotherapy has become expensive, toxic, and/or less effective due to drug resistance. on the other hand, many 3d structures in protein data bank (pdb) remain without function annotation. we need theoretical models to quickly predict biologically relevant parasite self proteins (psp), which are expressed differentially in a given parasite and are dissimilar to proteins expressed in other parasites and ... | 2011 | 21468430 |
| molecular characterization of anisakis larvae from fish caught off sardinia. | abstract anisakis spp. larvae are parasitic, and potentially zoonotic, nematodes transmitted by marine fish and cephalopods, which are the main intermediate hosts of the third larval stage. the accidental consumption of infected raw or poorly cooked fish may cause gastroenteric diseases and allergies in humans. the aim of the present study was to use pcr-rflp to define the occurrence, species variability, and host preferences of anisakis spp. larvae in fish caught off the coast of sardinia. necr ... | 2011 | 21501045 |
| seasonal occurrence of helminths in the anadromous fish coilia nasus (engraulidae): parasite indicators of fish migratory movements. | abstract to understand the seasonal migration of the anadromous coilia nasus , we attempted to identify the parasites infecting c. nasus and determine their seasonal occurrence. from june 2007 to july 2008, a survey of 775 c. nasus individuals from the estuary of the yangtze river and the coast of the east china sea revealed more than 7,300 parasites associated with the gills and alimentary tracts of c. nasus . the following 6 helminth taxa were identified, i.e., the monogeneans heteromazocraes ... | 2011 | 21506780 |
| population structure of three species of anisakis nematodes recovered from pacific sardine (sardinops sagax) distributed throughout the california current system. | abstract members of the anisakidae are known to infect over 200 pelagic fish species and have been frequently used as biological tags to identify fish populations. despite information on the global distribution of anisakis species, there is little information on the genetic diversity and population structure of this genus, which could be useful in assessing the stock structure of their fish hosts. from 2005 through 2008, 148 larval anisakids were recovered from pacific sardine (sardinops sagax) ... | 2011 | 21506810 |
| specific detection of angiostrongylus cantonensis in the snail achatina fulica using a loop-mediated isothermal amplification (lamp) assay. | angiostrongylus cantonensis, a rat lungworm, can cause eosinophilic meningitis and angiostrongyliasis in humans following ingestion of contaminated foods or intermediate/paratenic hosts with infective larvae. the snail achatina fulica is one of the important intermediate hosts of a. cantonensis and is commonly eaten by humans in some countries. in the present study, we developed a loop-mediated isothermal amplification (lamp) method for the specific detection of a. cantonensis in ac. fulica. pri ... | 2011 | 21515360 |
| different serum cytokine levels in chronic vs. acute anisakis simplex sensitization-associated urticaria. | the knowledge on immune mechanisms of chronic urticaria (cu) at the cytokine level is widely scarce. we compared pro- and anti-inflammatory as well as th1- and th2-associated serum cytokine levels in two phenotypes of cu: associated with (cu+) and without (cu-) sensitization against anisakis simplex, a ubiquitous fish parasite, that has been associated with acute urticaria in gastro-allergic anisakiasis (gaa) and with cu+. thirteen cu+ and 19 cu- patients were compared with 13 gaa patients and 1 ... | 2011 | 21554330 |
| prevalence and mean intensity of anisakis simplex (sensu stricto) in european sea bass (dicentrarchus labrax) from northeast atlantic ocean. | viscera and muscle of a total of 40 wild 1-2kg european sea bass (dicentrarchus labrax) from northeast atlantic (fao area 27) were examined for anisakidae larvae detection by digestion method. extracted parasites were counted and mean intensity was calculated. parasites were identified by genetic/molecular markers (allozymes and sequences analysis of the mtdna cox2 gene) as belonging to the species anisakis simplex (sensu stricto). in viscera, the main localisations of the larvae were under the ... | 2011 | 21620507 |
| complete mitochondrial genomes of baylisascaris schroederi, baylisascaris ailuri and baylisascaris transfuga from giant panda, red panda and polar bear. | roundworms of the genus baylisascaris are the most common parasitic nematodes of the intestinal tracts of wild mammals, and most of them have significant impacts in veterinary and public health. mitochondrial (mt) genomes provide a foundation for studying epidemiology and ecology of these parasites and therefore may be used to assist in the control of baylisascariasis. here, we determined the complete sequences of mtdnas for baylisascaris schroederi, baylisascaris ailuri and baylisascaris transf ... | 2011 | 21621593 |
| anisakis simplex (s.s.) larvae in wild alaska salmon: no indication of post-mortem migration from viscera into flesh. | the prevalence, mean intensity and distribution of anisakis nematode third-stage larvae (l3) in the muscle and viscera of wild-caught chum salmon oncorhynchus keta, pink salmon o. gorbuscha and sockeye salmon o. nerka were compared immediately after catch. salmon were collected during the fishing season in july 2007 in bristol bay and prince william sound close to cordova, alaska (usa). all fish were infected, and more than 90% of the nematode larvae were found in the edible muscle meat. the iso ... | 2011 | 21790067 |
| the effect of anti- anisakis simplex antibody levels on c3 and c4 complement components in human sera. | previously, an in vitro effect was observed on the complement system not only of the excretory-secretory products but also of somatic antigens from l3 anisakis simplex larvae. in the present work the effect of anti-a. simplex specific antibodies on c3 and c4 levels in human sera was investigated. up to 309 samples of sera were tested to determine levels of c3 and c4 and anti-a. simplex antibodies, including immunoglobulins igg, igm, iga and ige. significant differences were observed between leve ... | 2011 | 21729389 |
| synergism between prior anisakis simplex infections and intake of nsaids, on the risk of upper digestive bleeding: a case-control study. | the aim of this study was to investigate the relationship between prior anisakis infections and upper gastrointestinal bleeding (ugib), and its interaction with non-steroidal anti-inflammatory drug (nsaid) intake. | 2011 | 21738810 |
| Seroprevalence of antibodies against Anisakis simplex larvae among health-examined residents in three hospitals of southern parts of Korea. | The present study was performed to estimate the seroprevalence of larval Anisakis simplex infection among the residents health-examined in 3 hospitals in southern parts of Korea. A total of 498 serum samples (1 serum per person) were collected in 3 hospitals in Busan Metropolitan city, Masan city, and Geoje city in Gyeongsangnam-do (Province) and were examined by IgE-ELISA and IgE-western blotting with larval A. simplex crude extract and excretory-secretory products (ESP). The prevalence of anti ... | 2011 | 21738269 |
| A phage display system for the identification of novel Anisakis simplex antigens. | Anisakis simplex has been recognized as an important cause of disease in man and as a foodborne allergen source. Actually, this food-borne was recently identified as an emerging food safety risk including allergenic symptoms. This parasite contains a large variety of allergenic proteins enforcing the necessity to detect new allergens. Commonly, these efforts have been focused on the developing of cDNA libraries, where virtually all expressed mRNAs are present, by using immunoreactive patient ser ... | 2011 | 21893063 |
| viability and antigenicity of anisakis simplex after conventional and microwave heating at fixed temperatures. | inactivation of parasites in food by microwave treatment may vary due to differences in the characteristics of microwave ovens and food properties. microwave treatment in standard domestic ovens results in hot and cold spots, and the microwaves do not penetrate all areas of the samples depending on the thickness, which makes it difficult to compare microwave with conventional heat treatments. the viability of anisakis simplex (isolated larvae and infected fish muscle) heated in a microwave oven ... | 2011 | 22186053 |
| Infection levels and diversity of anisakid nematodes in blackspot seabream, Pagellus bogaraveo, from Portuguese waters. | The blackspot seabream, Pagellus bogaraveo, is a sparid fish of great economic importance in the northeast Atlantic. The main aim of this work was to assess the infection levels and diversity of anisakid nematodes parasitizing P. bogaraveo from Portuguese waters. The anisakid larvae were identified by polymerase chain reaction-restriction fragment length polymorphism analysis and ten different patterns were observed, four of which were not previously reported in the literature. Moreover, several ... | 2011 | 22139402 |
| Contracaecum rudolphii B: Gene content, arrangement and composition of its complete mitochondrial genome compared with Anisakis simplex s.l. | In the present study, we sequenced the complete mt genome (14,022bp) of parasitic nematode Contracaecum rudolphii B and its structure and organization compared with Anisakis simplex s.l. The mt genome of C. rudolphii B is slightly longer than that of A. simplex s.l. (13,916bp). C. rudolphii B mt genome is circular, and consists of 36 genes, including 12 genes for proteins, 2 genes for rRNA and 22 genes for tRNA. This genome contains a high A+T (70.5%) content. The mt gene order for C. rudolphii ... | 2011 | 22138443 |
| Anisakis simplex s.l. parasitization in mackerel (Scomber japonicus) caught in the North of Morocco - prevalence and analysis of risk factors. | Consumption of mackerel (Scomber japonicus) is very widespread in Morocco, where its production is an important economic pillar. We investigated the prevalence of infection by Anisakis spp. in mackerel caught in Moroccan waters, analyzing infection risk factors. The prevalence was generally higher in fish from the Atlantic (67.9%) than from the Mediterranean (57.0%), but they did not differ in the mean abundance, intensity, or prevalence of muscle parasitization. A. pegreffii was the predominant ... | 2011 | 21868119 |
| anisakis hypersensitivity in italy: prevalence and clinical features: a multicenter study. | anisakis simplex (as), a parasite in fish, is able to sensitize humans via the alimentary tract. the prevalence of hypersensitivity and allergy to as outside the iberian peninsula has not been investigated so far. we investigated anisakis hypersensitivity in different areas of italy. | 2011 | 21851362 |
| the anisakis allergy debate: does an evolutionary approach help? | allergic phenomena share common pathways with the immune response against helminth parasites. the definitions regarding allergens and their related concepts have their roots in the area of allergy research. the experience with the fish parasite anisakis simplex-associated allergic features still nurtures an open debate on the necessity of larvae being alive to induce allergic reactions such as urticaria or anaphylaxis. conceptual definitions of allergen, major allergen, as well as putatively cro ... | 2012 | 22079162 |
| identification of a 24 kda excretory secretory protein in anisakis simplex. | a gene coding for a 24 kda protein (22 u homologous; as22u) was isolated from the anisakis simplex third-stage larvae cdna library during expressed sequence tag analysis. as22u was 636 bp long, and was found to code for 212 amino acid residues with a calculated mass of 23.5 kda and a pi of 9.06. the as22u deduced amino acid sequence harbored a signal peptide region and 16 highly conserved cysteine residues, and it was identified in both the total extracts and excretory secretory (es) protein of ... | 2012 | 22001446 |
| a 24 kda excretory-secretory protein of anisakis simplex larvae could elicit allergic airway inflammation in mice. | we have reported that a 24 kda protein (22u homologous; as22u) of anisakis simplex larvae could elicit several th2-related chemokine gene expressions in the intestinal epithelial cell line which means that as22u may play a role as an allergen. in order to determine the contribution of as22u to allergic reactions, we treated mice with 6 times intra-nasal application of recombinant as22u (ras22u). in the group challenged with ras22u and ovalbumin (ova), the number of eosinophils in the bronchial a ... | 2011 | 22355204 |
| ige sensitisation to the fish parasite anisakis simplex in a norwegian population . a pilot study. | the reports on fish parasite anisakis simplex allergy have increased in countries with high fish consumption in the last decade. in norway, a high consumption country, the prevalence of immunoglobulin e (ige) sensitisation to a. simplex was still unknown. thus, our objective was to investigate the sensitisation prevalence in this country. at the haukeland university hospital, bergen, norway, two main groups of surplus serum samples were collected; one from newly recruited blood donors, and one f ... | 2011 | 22211721 |
| sensitization to anisakis simplex species in the population of northern morocco. | to investigate sensitization to third-stage anisakis simplex larvae in a randomly selected population in northern morocco. | 2012 | 23397674 |
| colonic polyp secondary to anisakis simplex. | | 2012 | 23268638 |
| characterisation of potential novel allergens in the fish parasite anisakis simplex. | the parasitic nematode anisakis simplex occurs in fish stocks in temperate seas. a. simplex contamination of fish products is unsavoury and a health concern considering human infection with live larvae (anisakiasis) and allergic reactions to anisakid proteins in seafood. protein extracts of a. simplex produce complex band patterns in gel electrophoresis and ige-immunostaining. in the present study potential allergens have been characterised using sera from a. simplex-sensitised patients and prot ... | 0 | 27110489 |
| expression and characterization of α-methylacyl coa racemase from anisakis simplex larvae. | larval excretory-secretory products of anisakis simplex are known to cause allergic reactions in humans. a cdna library of a. simplex 3rd-stage larvae (l3) was immunoscreened with polyclonal rabbit serum raised against a. simplex l3 excretory-secretory products to identify an antigen that elicits the immune response. one cdna clone, designated as α-methylacyl coa racemase (amacr) contained a 1,412 bp cdna transcript with a single open reading frame that encoded 418 amino acids. a. simplex amacr ... | 2012 | 22711931 |
| serine proteases of parasitic helminths. | serine proteases form one of the most important families of enzymes and perform significant functions in a broad range of biological processes, such as intra- and extracellular protein metabolism, digestion, blood coagulation, regulation of development, and fertilization. a number of serine proteases have been identified in parasitic helminths that have putative roles in parasite development and nutrition, host tissues and cell invasion, anticoagulation, and immune evasion. in this review, we de ... | 2015 | 25748703 |
| serine protease inhibitors of parasitic helminths. | serine protease inhibitors (serpins) are a superfamily of structurally conserved proteins that inhibit serine proteases and play key physiological roles in numerous biological systems such as blood coagulation, complement activation and inflammation. a number of serpins have now been identified in parasitic helminths with putative involvement in immune regulation and in parasite survival through interference with the host immune response. this review describes the serpins and smapins (small seri ... | 2012 | 22310379 |
| [cloning and expression of d-like aspartic protease of anisakis simplex]. | to clone and express the full length of d-like aspartic protease gene (asap) of the third stage larvae of anisakis simplex. | 2012 | 23072160 |
| scavenging hagfish as a transport host of anisakid nematodes. | hagfish are the most primitive craniates and scavengers, feeding on dead organisms as well as fish and invertebrates. hagfish play an important ecological role in recycling nutrients, helping to recycle biomass from the upper water column. we investigated 265 specimens of four hagfish species, including eptatretus burgeri, eptatretus yangi, eptatretus sheni and eptatretus taiwanae from northeastern taiwanese waters of the northwestern pacific from november 2013 to june 2014. eight species of ani ... | 2016 | 26872923 |
| occurrence and prevalence of fish-borne anisakis larvae in the spotted mackerel scomber australasicus from taiwanese waters. | anisakid nematodes have been found in a variety of marine fishes throughout the world and they are known to cause anisakiasis in human hosts. the present study investigated the prevalence of potentially zoonotic anisakid larvae in spotted mackerel caught from taiwanese waters where fish represents an important food sources. anisakis third-stage larvae (l3, n=502) were isolated from 250 spotted mackerel scomber australasicus. anisakis l3 larvae were divided morphologically into two types, anisaki ... | 2015 | 25733490 |
| experimental demonstration of pathogenic potential of anisakis physeteris and anisakis paggiae in wistar rats. | anisakis morphotype i is the principal etiologic agent of human anisakiasis, with differences in pathogenicity found between the anisakis simplex s.s. and a. pegreffii species; however, the role of morphotype ii larvae in this illness is not well understood. the purpose of this study is to verify the ability of morphotype ii larvae to invade tissues via the experimental infection of wistar rats, an animal model which simulates infection in humans. in the in vivo assay, 7.1% (4/56 l3 morphotype i ... | 2014 | 25240961 |
| occurrence of nematodes of the genus anisakis in mediterranean and atlantic fish marketed in sardinia. | anisakiasis is a gastrointestinal fish-borne zoonosis caused by the ingestion of third stage larvae of the genus anisakis. between january and december 2013, 1112 specimens of four commercial fish species (engraulis encrasicolus, merluccius merluccius, scomber colias and trachurus mediterraneus) marketed in sardinia (italy) were examined for anisakis sp. the overall prevalence of anisakis spp larvae was 39.9%, all morphologically identified as type i. scomber colias showed the highest prevalence ... | 2017 | 28299289 |
| anisakis simplex complex: ecological significance of recombinant genotypes in an allopatric area of the adriatic sea inferred by genome-derived simple sequence repeats. | the genus anisakis includes nine species which, due to close morphological resemblance even in the adult stage, have previously caused many issues in their correct identification. recently observed interspecific hybridisation in sympatric areas of two closely related species, anisakis simplex sensu stricto (s.s.) and anisakis pegreffii, has raised concerns whether a f1 hybrid generation is capable of overriding the breeding barrier, potentially giving rise to more resistant/pathogenic strains in ... | 2017 | 28057461 |
| survey on the presence of a. simplex s.s. and a. pegreffii hybrid forms in central-western mediterranean sea. | in this study, 1029 fish and cephalopod samples came from central-western mediterranean (fao 37.1.1 and fao 37.1.3) were analysed for anisakidae larvae research with the aim to identify possible hybridisations between anisakis pegreffii and anisakis simplex s.s. species. a total of 1765 larvae were detected, with prevalence values between 8.1% and 100%. the morphologic analysis revealed characters attributable to morphotype i of anisakis in 98.5% of the examined larvae, while 1.5% belonged to th ... | 2016 | 27568095 |
| anisakiasis mimics cancer recurrence: two cases of extragastrointestinal anisakiasis suspected to be recurrence of gynecological cancer on pet-ct and molecular biological investigation. | we report two cases of anisakiasis lesions that were initially suspected to be recurrence of gynecological cancer by positron emission tomography-computed tomography (pet-ct). both cases were extragastrointestinal anisakiasis that is very rare. | 2016 | 27112922 |
| no more time to stay 'single' in the detection of anisakis pegreffii, a. simplex (s. s.) and hybridization events between them: a multi-marker nuclear genotyping approach. | a multi-marker nuclear genotyping approach was performed on larval and adult specimens of anisakis spp. (n = 689) collected from fish and cetaceans in allopatric and sympatric areas of the two species anisakis pegreffii and anisakis simplex (s. s.), in order to: (1) identify specimens belonging to the parental taxa by using nuclear markers (allozymes loci) and sequence analysis of a new diagnostic nuclear dna locus (i.e. partial sequence of the ef1 α-1 ndna region) and (2) recognize hybrid categ ... | 2016 | 27046418 |
| anisakis pegreffii larvae in sea eels (astroconger myriaster) from the south sea, republic of korea. | anisakis simplex sensu stricto (s.s.), anisakis pegreffii, anisakis berlandi (=a. simplex sp. c), and anisakis typica are the 4 major species of anisakis type i larvae. in the republic of korea (korea), a. pegreffii, a. berlandi, and a. typica larvae in fish hosts has seldom been documented. in this study, molecular analysis was performed on anisakis larvae from the sea eels (astroconger myriaster), the major source of human anisakiasis in korea, collected from tongyeong city, a southern coastal ... | 2015 | 26174831 |
| macroparasites of allis shad (alosa alosa) and twaite shad (alosa fallax) of the western iberian peninsula rivers: ecological, phylogenetic and zoonotic insights. | samples of anadromous alosa alosa (clupeidae) (n = 163) and alosa fallax (clupeidae) (n = 223), caught in western iberian peninsula rivers from 2008 to 2013, were examined for buccal, branchial and internal macroparasites, which were identified using morphological and molecular methods. alosa alosa were infected with anisakis simplex s.s., anisakis pegreffii, hysterothylacium aduncum, rhadinorhynchus pristis, mazocraes alosae, hemiurus appendiculatus, ceratothoa italica and an unidentified ergas ... | 2015 | 26141433 |
| anisakis infection in allis shad, alosa alosa (linnaeus, 1758), and twaite shad, alosa fallax (lacépède, 1803), from western iberian peninsula rivers: zoonotic and ecological implications. | spawning individuals of allis shad, alosa alosa (linnaeus, 1758), and twaite shad, alosa fallax (lacépède, 1803), were sampled from three rivers on the atlantic coast of the iberian peninsula (ulla, minho, mondego) during 2008 to 2013 to assess the presence of the zoonotic marine parasite anisakis spp. larvae. the results revealed that both shad species were infected by third-larval stage anisakis simplex s.s. and anisakis pegreffii. the latter is reported in mixed infections in both shad specie ... | 2015 | 25810220 |
| fishing area and fish size as risk factors of anisakis infection in sardines (sardina pilchardus) from iberian waters, southwestern europe. | the sardine (sardina pilchardus) is a fish commonly consumed and appreciated in many countries, although they are more likely to be eaten fresh in western mediterranean countries such as spain, portugal, france or italy. a molecular epidemiological survey of sardines from 5 fishing areas of the spanish mediterranean (málaga, southern spain) and atlantic coasts (southern: cádiz and isla cristina; northern: a coruña and ondarroa) was carried out to determine the presence of anisakis spp. larvae. t ... | 2015 | 25770431 |
| pathogenic potential of two sibling species, anisakis simplex (s.s.) and anisakis pegreffii (nematoda: anisakidae): in vitro and in vivo studies. | the pathogenic potentials of two sibling nematodes anisakis simplex sensu stricto (s.s.) and a. pegreffii were compared by in vitro and in vivo studies. live third-stage larvae of each species were subjected to agar blocks made using pbs or rpmi-1640, overlaid with different supernatants (artificial gastric juice, pbs, and rpmi-1640), and their penetration ability was compared. their tolerance of artificial gastric juice was also tested. further, they were introduced into rats by gastric intubat ... | 2015 | 25685821 |
| haemoglobin, a new major allergen of anisakis simplex. | gastro-allergic anisakiasis and anisakis sensitisation associated chronic urticaria are diseases which differ in their ige and igg4 responses against both crude extract and specific allergens. anisakis and ascaris are closely related nematodes that usually cause problems with specificity in immunodiagnostics. in this study we measured ige and igg4 antibodies against anisakis simplex sensu lato (s. l.) and ascaris suum haemoglobins in sera of 21 gastro-allergic anisakiasis and 23 chronic urticari ... | 2015 | 25683373 |
| molecular diagnosis of cause of anisakiasis in humans, south korea. | anisakiasis in humans in south korea has been considered to be caused exclusively by the larvae of anisakis simplex sensu stricto and pseudoterranova decipiens. recently, however, dna sequencing of larvae from 15 of 16 anisakiasis patients confirmed the cause to be anisakis pegreffii infection. molecular analysis should be performed for all extracted larvae. | 2015 | 25625427 |
| molecular identification of anisakis and hysterothylacium larvae in marine fishes from the east china sea and the pacific coast of central japan. | anisakiasis is a human disease caused by the accidental ingestion of larvae belonging to the family anisakidae. three fish species, the small yellow croaker pseudosciaena polyactis, the mackerel pneumatophorus japonicus and the hairtail trichiurus haumela are important source for food products in the east china sea. the prevalence and the identification of anisakidae larvae in these fishes will benefit the prevention and control of anisakiasis. in this study, fish samples were obtained from fish ... | 2015 | 25617845 |
| genetic identification and distribution of the parasitic larvae of anisakis pegreffii and anisakis simplex (s. s.) in european hake merluccius merluccius from the tyrrhenian sea and spanish atlantic coast: implications for food safety. | the consumption of the hake merluccius merluccius is widespread in european countries, where this fish has a high commercial value. to date, different larval species of anisakis have been identified as parasites in m. merluccius from european waters, anisakis pegreffii and anisakis simplex (s. s.) being the two most common. the aim of the study is to present data on the occurrence of anisakis spp. larvae in the viscera and flesh of m. merluccius. consequently, the distribution and infection rate ... | 2015 | 25584776 |
| three anisakis spp. isolated from toothed whales stranded along the eastern adriatic sea coast. | knowledge concerning cetacean ecology in the mediterranean is limited but important for sustainable planning and enforcement of appropriate conservation measures. any information that might help to elucidate their ecology is essential. we explored the population and genetic structures of anisakis spp. nematodes isolated from four toothed whale species - bottlenose dolphins (tursiops truncatus), striped dolphins (stenella coeruleoalba), risso's dolphins (grampus griseus) and cuvier's beaked whale ... | 2015 | 25449948 |
| occurrence of anisakid nematode larvae in chub mackerel (scomber japonicus) caught off korea. | chub mackerel (scomber japonicus) is a pelagic fish species widely distributing in the indo-pacific and a commercially important fish species in korea. it is known to harbor anisakid nematodes larvae, and ingesting the raw or undercooked fish can accidentally cause human infection. in this study, we isolated the nematode larvae in 417 chub mackerel caught from 7 sampling locations around the korean peninsula in 2011 and 2012, and identified them by pcr-rflp of the its (internal transcribed space ... | 2014 | 25268324 |
| anisakidae in fishing products sold in sicily. | one of the parasite diseases associated with the consumption of raw fish that occurs with some frequency is the anisakiasis, a human disease caused by the accidental ingestion of larval nematodes of the genus anisakis, family anisakidae. at the national reference centre for anisakiasis (c.re.n.a.) from october 2012 to february 2013, a number of 231 bony fish (trichiuridae, engraulidae, scombridae and clupeidae) were received from different fishing sites in sicily. anisakis pegreffii is the main ... | 2014 | 27800344 |
| molecular identification of anisakis species (nematoda: anisakidae) from marine fishes collected in turkish waters. | anisakid nematodes are important etiological agents for zoonotic human anisakiasis (or anisakidosis). these parasites in the turkish waters still remain unexplored. this study aims the molecular identification of anisakis species in turkey's coast from black, aegean and mediterranean sea and specifically to screen for zoonotic species in commonly commercialized a total of 1145 fish belonging to 31 different species using both polymerase chain reaction-restriction fragment length polymorphism ana ... | 2014 | 24485564 |
| ecology and genetic structure of zoonotic anisakis spp. from adriatic commercial fish species. | consumption of raw or thermally inadequately treated fishery products represents a public health risk, with the possibility of propagation of live anisakis larvae, the causative agent of the zoonotic disease anisakidosis, or anisakiasis. we investigated the population dynamics of anisakis spp. in commercially important fish-anchovies (anisakis), sardines (sardina pilchardus), european hake (merluccius merluccius), whiting (merlangius merlangus), chub mackerel (scomber japonicus), and atlantic bl ... | 2014 | 24317085 |
| presence of anisakid larvae in the european anchovy, engraulis encrasicolus, fished off the tyrrhenian coast of central italy. | a survey was carried out to determine the prevalence of anisakid nematode larvae in european anchovy (engraulis encrasicolus) fished off the tyrrhenian coast of central italy. from february through july 2012, 1,490 specimens of e. encrasicolus caught in three different fishing areas (off civitavecchia, anzio, and gaeta in the northern, central, and southern lazio region of italy, respectively) were tested for the presence of anisakid larvae, both by visual microscopic inspection and enzymatic di ... | 2013 | 23992513 |
| molecular characterization and comparison of four anisakis allergens between anisakis simplex sensu stricto and anisakis pegreffii from japan. | it remains unclear whether allergens are the same among the sibling species of anisakis simplex sensu lato. this study was carried out to compare the amino acid sequences of three major (ani s 1, 2 and 12) and one minor (ani s 9) anisakis allergens between a. simplex s.s. and anisakis pegreffii. we found 2 (out of 163), 1 (out of 869), and 29 (out of 266) amino acid variable sites for ani s 1, 2, and 12, respectively. however, as both intra- and inter-species variations were present at the same ... | 2013 | 23742817 |
| experimental comparison of pathogenic potential of two sibling species anisakis simplex s.s. and anisakis pegreffii in wistar rat. | there are little data available on the pathology caused by the sibling species anisakis simplex s.s. and anisakis pegreffii. the differences shown in their ability to penetrate the muscle of fish may also be manifested in humans. the purpose of this study is to confirm possible differences in pathogenicity between a. simplex s.s. and a. pegreffii using an experimental model which simulates infection in humans. | 2013 | 23724976 |
| molecular characterisation of anisakidae larvae from fish in adriatic sea. | in the present study, anisakids from: tuna (thunnus thynnus) fattened in the croatian farm in middle adriatic sea, three different feral fish species caught near tuna farm (trachurus trachurus, scomber japonicus and oblada melanura) and fish marketed in croatia (t. trachurus) were analysed by morphology and molecular methods. larvae were identified to the species level by pcr restriction fragment length polymorphism and characterised by sequencing of nuclear (internal transcribed spacer) and mit ... | 2012 | 22983167 |
| anisakis simplex sensu stricto and anisakis pegreffii: biological characteristics and pathogenetic potential in human anisakiasis. | anisakiasis is one of the most common fishborne helminthic diseases in japan, which is contracted by ingesting the larvae of the nematode anisakis spp. carried by marine fish. anisakis simplex sensu stricto (s.s.) and a. pegreffii are the dominant species in fish caught offshore japan. the present study aimed to identify the anisakid species infecting japanese patients and determine whether there is any difference in the pathogenetic potential of a. simplex (s.s.) and a. pegreffii. in total, 41 ... | 2012 | 22545961 |
| molecular identification of anisakis spp. from fishes collected in the tyrrhenian sea (nw mediterranean). | the accurate identification of anisakid nematodes at any life cycle stage is important both to deepen the knowledge on their taxonomy, ecology, epidemiology and for diagnosis and control, as larval stages cause a clinical disease in humans known as anisakidosis. with the aim to investigate the presence of anisakid larvae, specimens of horse mackerel, trachurus trachurus (linnaeus, 1758), silver scabbardfish, lepidopus caudatus (euphrasen, 1788), european anchovy, engraulis encrasicolus (linnaeus ... | 2012 | 22360842 |