| effect of gas phase on carbohydrate metabolism in ascaris suum larvae. | the in vitro metabolism of [1-13c]glucose by ascaris suum third and fourth-stage larvae was analyzed under different gas phases using 13c nuclear magnetic resonance spectroscopy (13c-nmr). third-stage larvae (l3) incubated under a gas phase of 85% n2/5% o2/10% co2 produced trace amounts of [13c]succinate, and molted to fourth-stage larvae (l4) between days 3 and 4 in vitro. however, they appeared to arrest as l3s when incubated under air, or 85% n2/5% o2/10% co2 in the presence of 2 mm potassium ... | 1989 | 2509908 |
| divalent hapten-induced intestinal anaphylaxis in the mouse: uptake and characterization of a bystander protein. | we examined the mucosal barrier function during anaphylaxis induced by the hapten n,n'-di-2,4,dinitrophenyl-lysine (di-dnp-lysine) in bdf1 female mice immunized with dinitrophenylated ascaris suum extract. immunized mice were gavaged with 10 mg or 50 mg of ovalbumin (ova) with or without n,n'-di-2,4,-dnp-lysine (di-dnp-lysine). animals that received di-dnp-lysine underwent anaphylaxis and were observed to have significantly greater serum concentrations of immunoreactive ova (iova) than control m ... | 1989 | 2514138 |
| an abattoir survey of the prevalence of gastro-intestinal helminths and stephanurus dentatus in pigs in belize. | in an abattoir survey in belize 137 pigs were examined for gastro-intestinal helminths and stephanurus dentatus. hyostrongylus rubidus, physocephalus sexalatus, globocephalus species and trichostrongylus colubriformis were recorded for the first time. ascarops strongylina, ascaris suum, macracanthorynchus hirudinaceus, strongyloides ransomi, trichuris suis, oesophagostomum species and s. dentatus were also found. oesophagostomum species (45%) and s. dentatus (42%) were the most prevalent. the pr ... | 1989 | 2529683 |
| production and distribution of immunoglobulin-bearing cells in the intestine of young pigs infected with ascaris suum. | pigs of 10 days-1 month old were orally infected with eggs of ascaris suum at different rates and inoculation schedules. histological sections from various parts of the small intestines were prepared to observe the production and localization of immunoglobulin-bearing cells. fluorescent antibody and immunoperoxidase staining methods were used to determine the number of igm-, iga- and igg-producing plasma cells in the intestinal lamina propria. significant increases in immunoglobulin-bearing cell ... | 1989 | 2531490 |
| effects of combined receptor antagonists of leukotriene d4 (ltd4) and platelet-activating factor (paf) on rhesus airway responses to ltd4, paf and antigen. | rhesus monkeys with ige-mediated asthmatic-type responses to ascaris suum antigen were pretreated with a combination of 2 receptor antagonists to leukotriene d4 (ltd4) and platelet-activating factor (paf). this combination of anti-ltd4 and anti-paf was shown to inhibit airway responses to either ltd4 or paf in separate experiments in these ascaris airway-reactive animals. when the same combination of ltd4 and paf receptor antagonists was used to pretreat the same animal prior to aerosol challeng ... | 1989 | 2542169 |
| purification and properties of the cyclic 3',5'-amp-binding protein from the muscle of the nematode ascaris suum. | the cyclic 3',5'-amp-binding protein was isolated from the muscle of ascaris suum and purified to apparent homogeneity. it migrated as a protein with a relative mr 54,000 on electrophoresis under denaturing conditions. on gel filtration columns it was eluted at a volume corresponding to a protein of mr greater than 200,000 under conditions which kept the cyclic 3',5'-amp-binding property intact. the purified catalytic subunit of protein kinase from ascaris and the c subunit of cyclic 3',5'-amp-d ... | 1989 | 2543296 |
| effect of nedocromil sodium on antigen-induced airway responses in allergic sheep. | we studied the effects of nedocromil sodium and sodium cromoglycate on early and late bronchial responses, the airway inflammation associated with the late response to inhaled ascaris suum antigen in allergic sheep in vivo, and the antigen-induced contractile responses of sheep tracheal smooth muscle in vitro. in addition, we examined the effect of nedocromil sodium on the development of antigen-induced airway hyper-responsiveness in this model. pretreatment with either nedocromil sodium or sodi ... | 1989 | 2547569 |
| purification and properties of cyclic-3',5'-gmp-dependent protein kinase from the nematode ascaris suum. | a cyclic-3',5'-gmp-dependent protein kinase was purified 7400-fold from the reproductive tract of female ascarids to a specific activity of 718 nmol min-1 mg-1 (histone as substrate). the yield of the preparation was 25%. the enzyme protein obtained was homogeneous as judged by isoelectrofocusing and polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulfate. the native enzyme behaved as a dimer of two 82-kda subunits in gel permeation chromatography on superose 12. the protein ... | 1989 | 2549874 |
| gaba receptors on the somatic muscle cells of the parasitic nematode, ascaris suum: stereoselectivity indicates similarity to a gabaa-type agonist recognition site. | 1. the gamma-aminobutyric acid (gaba) receptors on the somatic muscle cells of ascaris, which mediate muscle cell hyperpolarization and relaxation, have been characterized by use of intracellular recording techniques. 2. these receptors are like mammalian gabaa-receptors in that the response is mediated by an increase conductance to chloride ions. the gabaa-mimetic, muscimol, has a relative potency of 0.40 +/- 0.02 (n = 3) compared to gaba. 3. the stereoselectivity of the gaba receptor on ascari ... | 1989 | 2556203 |
| role of arachidonic acid metabolites in allergen-induced late responses. | the sheep model of allergic airway disease shares many pathophysiological similarities with allergic airway disease in humans. studies performed in this animal model present strong evidence that the release of arachidonic acid metabolites plays an important role in the development of late bronchial responses to antigen challenge. the release of leukotrienes through the lipoxygenase pathway during the acute bronchial obstruction after inhalation of ascaris suum antigen represents the key factor f ... | 1989 | 2557658 |
| possible mode of action of toltrazuril: studies on two eimeria species and mammalian and ascaris suum enzymes. | the anticoccidial properties of toltrazuril in eimeria falciformis-infected mice were potentiated by the simultaneous application of pyrimethamine, trimethoprim, or sulfadimidine. the same drugs potentiate the effect of toltrazuril by killing e. tenella schizonts in chicken kidney-cell cultures. activities of some enzymes of the respiratory chain, such as succinate-cytochrome c reductase and nadh oxidase and succinate oxidase from mouse liver, were reduced in the presence of toltrazuril. the sam ... | 1989 | 2560189 |
| effect of picumast dihydrochloride on antigen-induced early and late airway responses in allergic sheep. | in this study the effects of picumast dihydrochloride (3,4-dimethyl-7-[4-(4-chlorobenzyl)piperazine-1-yl]propoxycoumarine++ + dihydrochloride) a novel antiallergic agent, on antigen-induced early and late responses in allergic sheep were examined. for all studies picumast dihydrochloride was administered as an aerosol approximately 20 min before airway challenge with ascaris suum antigen. allergic sheep were challenged on four occasions each greater than or equal to 14 days apart: one placebo tr ... | 1989 | 2576357 |
| serodiagnosis of ocular toxocariasis: a comparison of two antigens. | this study was designed to compare the sensitivity and specificity of enzyme linked immunosorbent assay (elisa) for the serodiagnosis of ocular toxocariasis using toxocara canis embryonated egg antigen (tee) and toxocara excretory-secretory or exoantigen (tex) produced in vitro. tee and tex elisa were comparably sensitive, but tex elisa was better able to discriminate between serum samples from patients with ocular toxocariasis and those from patients with retinoblastoma. in addition, preabsorpt ... | 1985 | 2578482 |
| gamma-aminobutyric acid- and piperazine-activated single-channel currents from ascaris suum body muscle. | gamma-aminobutyric acid (gaba)- and piperazine-activated single-channel currents were recorded from the bag region of the somatic muscle of the nematode parasite ascaris suum. cell-attached and outside-out patch-clamp techniques were used. clean membranes were routinely prepared using collagenase. gaba (concentrations greater than 1 microm) or piperazine (concentrations greater than 200 microm) applied to the extracellular surface of the patches brought about the opening of channels producing re ... | 1985 | 2579701 |
| exposure to 1 ppm ozone attenuates the immediate antigenic response of canine peripheral airways. | the effect of oxidant exposure on the immediate airway response to immunologic challenge is controversial. we investigated the response of canine peripheral airways to antigen aerosol, 1-3 h and 24 h after a 5-min exposure to 1 ppm ozone. in dogs that were natively sensitive to ascaris suum antigen, resistance to flow through the collateral system (rcs) was measured using the wedged bronchoscope technique. in eight dogs, four sublobar segments of each lung were wedged: two were exposed to ozone ... | 1989 | 2585539 |
| preexposure to ozone blocks the antigen-induced late asthmatic response of the canine peripheral airways. | the influence of exposure of the airways to ozone on acute allergic responsiveness has been investigated in several species. little is known, however, about the effect of this environmental pollutant on the late asthmatic response (lar) in animals in which it is exhibited. the purpose of this study was to evaluate this effect in the canine peripheral airways and to assess the potential role of mast cells in modulating the effect. a series of experiments on seven mongrel dogs demonstrated that th ... | 1989 | 2585540 |
| [the biological activity of the lipopolysaccharide complexes in ascarids]. | lipopolysaccharide complex with chemical structure similar to that of complexes of microbial origin was isolated from ascaris suum by not phenol-aqueous extraction. | 1989 | 2615713 |
| ym461, a paf antagonist, blocks antigen-induced late airway responses and airway hyperresponsiveness in allergic sheep. | we examined the effect of an orally active antagonist, ym461, of platelet activating factor (paf) on antigen-induced early and late airway responses and on the development of airway hyperresponsiveness 24 h after challenge in allergic sheep. early and late airway responses were determined by measuring specific lung resistance (srl) before and periodically after challenge. airway responsiveness was determined from the slopes of dose-response curves of srl vs. increasing doses of carbachol aerosol ... | 1989 | 2620695 |
| af1, a sequenced bioactive neuropeptide isolated from the nematode ascaris suum. | an fmrfamide-like neuropeptide, named af1, was isolated from head extracts of the nematode ascaris suum using five steps of hplc. af1 is a heptapeptide with the amino acid sequence lys-asn-glu-phe-ile-arg-phe-nh2. synthetic af1 (10(-9) to 10(-7) m) rapidly and reversibly abolished slow membrane potential oscillations of identified ventral and dorsal inhibitory motoneurons and selectively reduced their input resistances. synaptic transmission was not blocked. in intact ascaris, af1 inhibited loco ... | 1989 | 2627377 |
| [alpha-amylase activity in serum and some organs of guinea pigs with larval ascariasis]. | the studies were carried out on 40 guinea pig males weighting about 230 g. the experimental animals were infected with 7000 invasive eggs of ascaris suum. on the 3-rd, 7-th and 10-th day after infection the activity of alpha-amylase was estimated according to caraway's method in serum, liver, pancreas, lungs, kidneys and spleen. the infection of guinea pigs results in increased activity of enzyme in serum and spleen, and decreased in pancreas, liver, kidneys and lungs. on the 3-rd day after infe ... | 1989 | 2633502 |
| [trypsin and alpha-amylase activity in the pancreas of guinea pigs. i. the influence of infective dose and duration of infection by ascaris suum goeze, 1782]. | the studies were carried out on guinea pig males weighting about 280 g. the animals were infected with 5000 or 20,000 invasive eggs of ascaris suum. on the 3-rd, 7-th, 10-th and 14-th day of invasion the animals were dissected. lungs were weighted, and the number of ascaris larvae was estimated using baermann method. the activity of alpha-amylase (fennel's method) and trypsin (anson's method) were examined in pancreas homogenate. the infection of guinea pigs with a. suum larvae resulted in a dec ... | 1989 | 2633503 |
| the pyruvate dehydrogenase complex from anaerobic mitochondria of the parasitic nematode ascaris suum: stoichiometry of phosphorylation and inactivation. | | 1989 | 2634345 |
| carboxypeptidase inhibitors from ascaris suum: the primary structure. | the carboxypeptidase a inhibitor from ascaris suum was isolated from aqueous extracts by affinity chromatography toward immobilized carboxypeptidase a. the amino acid sequence is dqvrkclsdt10dctngekcvq20knkicstive30iqrcekehft40ipcksnndcq50vwahekicn k60lpwgl65 . the carboxypeptidase a inhibitor is not homologous with the chymotrypsin/elastase or trypsin inhibitors from ascaris, but shows homology in a 9-residue internal sequence with the 37/39-residue carboxypeptidase inhibitors from tomato and p ... | 1989 | 2648995 |
| synergistic effect of migrating ascaris larvae and escherichia coli in piglets. | the effect of intestinal flora on the establishment, development and pathogenicity of ascaris suum larvae in piglets (large white breed) was investigated. the infected piglets with ascaris and escherichia coli showed signs of pneumonia, cough with respiratory difficulties initially even though these moderated with time. they lost appetite and showed signs of unthriftiness with loss of weight. the packed cell volume was normal but the differential leucocyte counts of the pigs infected with ascari ... | 1989 | 2656846 |
| physicochemical characterization and monoclonal and polyclonal antibody recognition of baylisascaris procyonis larval excretory-secretory antigens. | baylisascaris procyonis larval excretory-secretory (es) antigens consisted of complex glycoproteins ranging from 10 kda to over 200 kda as determined by sodium dodecyl sulfate polyacrylamide gel electrophoresis and lectin binding. five monoclonal antibodies (bapr1-bapr5) produced against b. procyonis es antigens were assayed by western blotting with larval es antigens from b. procyonis, baylisascaris melis, baylisascaris transfuga, ascaris suum, and toxocara canis. bapr1 and bapr2 recognized per ... | 1989 | 2668490 |
| antigen-coated sepharose beads induce airway eosinophilia and airway hyperresponsiveness in cynomolgus monkeys. | a method of inducing sustained airway eosinophilia and airway hyperresponsiveness in primates has been developed. our method utilizes a series of intratracheal instillations of ascaris suum-coated sepharose 4b beads (3 x 10(5] administered once a week for four weeks. five cynomolgus monkeys (macaca fascicularis) demonstrating a naturally occurring skin and respiratory sensitivity to ascaris suum extract were studied. airway cell composition was measured by bronchoalveolar lavage (bal), and airwa ... | 1989 | 2675702 |
| detection of giardia antigen in stool samples by a semi-quantitative enzyme immunoassay (eia) test. | a semi-quantitative enzyme immunoassay (eia) test for the detection of giardia intestinalis antigen in faeces was developed. in order to avoid unspecific reactions due to anticalf serum activity, igg fractions of anti-giardia rabbit and sheep sera were purified from antibovine antibodies by immunoadsorption. faecal specimens tested in the assay were mixed with normal horse serum to avoid unspecific and proteolytic effects of stool components. out of a range of bacterial and parasitic antigens te ... | 1989 | 2685985 |
| [immunoenzymatic evaluation of serum ige in onchocerciasis in chiapas, mexico]. | concentrations of total serum ige were measured by an immunoenzymatic assay (phadezym-prist) in 60 mexican onchocerciasis patients. in order to detect ige antibodies against adult onchocerca volvulus antigens, separately six onchocerciasis sera were depleted of ige antibodies by using a mixture of onchocerca gutturosa, ascaris suum and fasciola hepatica antigenic extracts coupled with sepharose 4b. additionally, the sera were incubated with an adult o. volvulus antigenic extract coupled with sep ... | 1989 | 2697095 |
| responses of nfr/n inbred mice to very low-dose infections with ascaris suum. | mice were evaluated for immunological responses and resistance to challenge infections after single infections. when given infections simultaneously with thiabendazole, larval migration to the liver and lungs was blocked but not when mice were given infections only. enlargement of peyer's patches was observed after infection followed by increased numbers of iga-containing cells in the lamina propria of the intestines and increased levels of hepatobiliary iga. ig-containing cells were variable in ... | 1989 | 2707966 |
| succinate-dependent energy generation in ascaris suum mitochondria. | phosphorylation in isolated ascaris suum mitochondria was much greater in the presence of malate than succinate, but, in the absence of added adenine nucleotides, incubations in succinate resulted in substantial elevations in intramitochondrial atp levels. succinate-dependent phosphorylation was stimulated aerobically and this stimulation was due almost entirely to a site i, rotenone-sensitive, phosphorylation. increased substrate level phosphorylation, coupled to propionate formation, or additi ... | 1989 | 2710162 |
| physico-chemical characteristics of superoxide dismutase in ascaris suum. | 1. three sod isoenzymes obtained from purified extracts of ascaris suum were characterized. 2. the physico-chemical characteristics studied were: optimum ph, methods of preservation of enzymatic activity, molecular weight, and the u.v. and visible light absorption spectra. 3. the optimum ph for the cu, zn sod i and ii was 10.2 and 10.1 for the mn sod. 4. the extracts retained their levels of activity longer at -70 degrees c, and after lyophilization. the mn sod was more labile than the cu, zn so ... | 1989 | 2721158 |
| effect of fenbendazole and pyrantel tartrate on the induction of protective immunity in pigs naturally or experimentally infected with ascaris suum. | an experiment was conducted with 96 weanling pigs (avg initial wt 18.5 kg) divided into six treatment with two replicates of eight pigs each. pigs in treatments 1, 2 and 3 were penned in outside pens with dirt lots that previously were contaminated with a. suum ova to induce a natural ascaris infection. pigs in treatments 4, 5 and 6 were penned in an open-front building with solid concrete floors and were experimentally infected with 2,000 embryonated a. suum. ova on d 1, 15 and 29 of the experi ... | 1989 | 2722696 |
| airway responses to inhaled ascaris suum antigen in naturally sensitized beagle dogs. | to characterize the airway responses to inhaled ascaris suum antigen in a group of adult beagle dogs with cutaneous ascaris sensitivity, 9 dogs were challenged with aerosolized ascaris antigen and 6 were subsequently challenged 1 to 2 weeks later with aerosolized saline. changes in lung resistance, dynamic lung compliance, and responsiveness to aerosolized histamine were measured during the ensuing 6 hours. inhalation of ascaris antigen produced immediate bronchoconstriction, but no spontaneous ... | 1989 | 2727396 |
| evidence for platelet-activating factor secretion during immune activation in dogs. | to elucidate the potential physiological significance of platelet-activating factor (paf) in acute bronchoconstriction, we studied the effect of ascaris suum antigen on the tachyphylactic response to paf in 15 natively allergic mongrel dogs in vivo. active bronchial tension was measured isometrically, and mediator secretion was measured as the arteriovenous difference (avd) in plasma concentration across the lungs. administration of paf into the bronchial artery caused dose-related contraction i ... | 1989 | 2732178 |
| electron-transfer flavoprotein from anaerobic ascaris suum mitochondria and its role in nadh-dependent 2-methyl branched-chain enoyl-coa reduction. | electron-transfer flavoprotein was purified to apparent homogeneity from mitochondria of the parasitic nematode, ascaris suum. the native molecular weight of the enzyme was 70,000, as estimated by gel filtration, and it migrated as two bands with apparent subunit molecular weights of 37,000 and 31,500 during sodium dodecylsulfate polyacrylamide gel electrophoresis. the enzyme exhibited an absorption coefficient for the bound fad of 13.5 mm-1.cm-1 at 436 nm and a protein/flavin (270 nm/436 nm) ra ... | 1989 | 2736251 |
| the effect of an orally active leukotriene (lt) antagonist ym-16638 on antigen-induced early and late airway responses in allergic sheep. | in this study we examined the effects of an orally active leukotriene (lt) antagonist ym-16638 [[5-[[3-(4-acetyl-3-hydroxy-2-propyl-phenoxy)propyl]thio]-1,3,4- thiadiazol-2-yl]thio] acetic acid on antigen-induced early and late responses in allergic sheep. for all studies ym-16638 was administered via intragastric tube 1 h before airway challenge with ascaris suum antigen. six allergic sheep were challenged on four occasions (2 control and 2 drug trials) each greater than or equal to 14 days apa ... | 1989 | 2740413 |
| a possible role for paf in allergen-induced late responses: modification by a selective antagonist. | we determined whether platelet-activating factor (paf) plays a role in allergen-induced airway responses by studying the effects of a selective paf antagonist web-2086 on antigen-induced early and late airway responses in allergic sheep. in seven sheep, inhaled ascaris suum produced significant early (282%) and late (176%) increases in specific lung resistance (srl). web-2086 (1 mg/kg iv) given 20 min before antigen challenge did not affect the early response, but the peak late increase in srl w ... | 1989 | 2745300 |
| serotonin receptors in the tissues of adult ascaris suum. | serotonin (5-hydroxytryptamine, 5-ht) receptors in the muscle and intestinal tissues of adult ascaris suum have been investigated. [3h] lysergic acid diethylamide (lsd) exhibited specific and saturable binding to membranes prepared from both intestine and muscle. the intestinal tissue membranes had an equilibrium dissociation constant (kd) of 2.70 nm for lsd and a kd of 2.50 microm for 5-ht. as compared to the intestine, the muscle membranes had comparatively higher affinity for both lsd (kd = 1 ... | 1989 | 2747743 |
| accurate and efficient rna polymerase iii transcription in a cell-free extract prepared from ascaris suum embryos. | high-speed supernatant (s100) extracts derived from homogenized ascaris suum embryos efficiently transcribe added rna polymerase iii templates including cloned 5s rrna genes of the filarial parasite brugia malayi. several criteria, including two-dimensional rnase t1 oligonucleotide fingerprint analysis, indicate that in vitro transcription is accurately initiated and terminated. | 1989 | 2747746 |
| retrovesicular ganglion of the nematode ascaris. | the nematode nervous system is distinguished by the small number and morphological simplicity of its neurons. recently, the shapes and synaptic interactions of each of the 302 neurons in the small free-living nematode, caenorhabditis elegans, have been determined from reconstructions of serial sections by electron microscopy. comparable anatomical studies of the large parasitic nematode ascaris have concentrated on the dorsal and ventral nerve cords where reconstructions of motor neurons by ligh ... | 1989 | 2754041 |
| some aspects of the pharmacology and physiology of the ascaris suum muscle. | out of 11 agonists, including such agents as bradykinin, prostaglandin e2, eledoisin, only acetylcholine (ach) caused the isolated exposed ascaris muscle to contract during treatment for 30 sec and at 10(-7) g/ml (or lower concentrations). the possibility of using this preparation for the identification and quantification of ach in biological tissues and fluids was highlighted. neostigmine, but not physostigmine, augmented ach activity. this suggests that cholinesterases present in the ascaris m ... | 1989 | 2757466 |
| a paf antagonist blocks antigen-induced airway hyperresponsiveness and inflammation in sheep. | we studied the effects of web-2086, a specific antagonist of platelet-activating factor (paf), on the development of antigen-induced airway hyperresponsiveness and inflammation in sheep (n = 8). for these studies, airway responsiveness was determined from slopes of carbachol dose-response curves (drc) performed at base line (prechallenge) and 2 h after ascaris suum antigen challenges in the following three protocols: 1) antigen challenge alone (control trial), 2) web-2086 (1 mg/kg iv) given 30 m ... | 1989 | 2759969 |
| influence of helminth parasite exposure and strategic application of anthelmintics on the development of immunity and growth of swine. | infection of pigs with the intestinal roundworm parasite ascaris suum and strategic application of anthelmintic drugs during the growing phase of development were observed for specific effects on 1) development of immunity in feeder pigs and 2) growth rate during the finishing phase. management treatments included maintenance in a parasite-free concrete environment, maintenance in a concrete environment and inoculation with 1,000 infective a. suum eggs every other day over a 52-d period, and mai ... | 1989 | 2768122 |
| preparation of monoclonal antibody against angiostrongylus cantonensis antigen. | monoclonal antibodies (mab) against a cantonensis were produced through fusion of immunised spleen cells from balb/c mice with ns-1 myeloma cells at a ratio of 10:1. the successful fusion rate on the 3rd day of fusion was 90.1%. ten mab were characterised, six of which were igg1 and the remaining four were igg2a, igg2b, igm and iga respectively. among 6 igg1 mab, four were a. cantonensis-specific, of which three reacted to adult worm antigen only and one reacted to both adult worm and juvenile w ... | 1989 | 2772697 |
| a novel type of putrescine (diamine)-acetylating enzyme from the nematode ascaris suum. | a cytosolic enzyme catalysing the acetylation of the diamines putrescine, cadaverine, 1,3-diaminopropane and 1,6-diaminohexane has been partially purified from reproductive tissue of the intestinal parasitic nematode ascaris suum. the enzyme formed n-acetylated derivatives of the above diamines when incubated in the presence of acetyl-coa. the michaelis constants (km) for the above diamines were 0.25 nm, 0.1 mm, 1.25 mm and 0.4 mm respectively, and the apparent km for acetyl-coa was 7.7 microm. ... | 1989 | 2775189 |
| efficacy of an in-feed ivermectin formulation against gastrointestinal helminths, lungworms, and sarcoptic mites in swine. | the efficacy of ivermectin as an in-feed formulation was evaluated against naturally acquired gastrointestinal helminths, lungworms, and sarcoptic mites (experiment 1; n = 24) and against induced infection with intestinal nematodes (experiment 2; n = 24) in pigs. treatments consisted of ivermectin administered in feed at concentrations calculated to provide 100 or 200 micrograms/kg of body weight/d for 7 days or of nonmedicated feed (controls) for 7 days. at concentration of 100 micrograms of iv ... | 1989 | 2782722 |
| eosinophilia in mouse regulated by multiple factors following repeated stimulations with ascaris eggs into the peritoneal cavity. | repeated injections of viable eggs of ascaris suum into the peritoneal cavity of balb/c mice induced marked peritoneal eosinophilia (9.3 x 10(6) cells/mouse, 52.8%) without infection. extracted antigen (asc) and eggs that had been killed also were able to induce marked eosinophilia; however, their levels were significantly lower (p less than 0.001). this experimental system was able to demonstrate how various multiple factors cumulatively affected the degree of peritoneal eosinophilia. these fac ... | 1989 | 2786507 |
| nematode parasite infections of domestic pigs in a sub-tropical and high-rainfall area of india. | fourteen hundred and ninety-six domestic pigs, sus scrofa domestica l., from a sub-tropical and high-rainfall area of india were examined over a period of 18 months to ascertain their nematode parasite spectrum. the eleven species recovered, arranged in descending order of prevalence, are as follows: ascaris suum, oesophagostomum dentatum, bourgelatia diducta, stephanurus dentatus, globocephalus connorfilii, physocephalus sexalutus, ascarops dentata, a. strongylina, pseudocruzia orientalis, seta ... | 1989 | 2787072 |
| effect of dietary magnesium on ascaris suum infections of mice. | low amounts of dietary magnesium affected the inflammatory tissue response in nonimmunized mice differently than in immunized mice. eosinophil numbers and lpl activity in lung tissue following infection with a. suum larvae were altered by the level of magnesium in the diets of mice. average or higher dietary levels of magnesium resulted in decreased numbers of lung larvae indicating an overall protective effect. increases in eosinophil numbers or lpl activity were not directly related to the num ... | 1989 | 2789853 |
| substrate activation by malate induced by oxalate in the ascaris suum nad-malic enzyme reaction. | substrate activation of the rate of the nad-malic enzyme reaction by malate is obtained in the presence but not in the absence of oxalate. the substrate activation is a result of competition between malate and oxalate for the e.nadh complex, with malate binding to the form of the complex unprotonated at an enzyme group with a pk of 4.9 and oxalate binding preferentially to the protonated form. the off-rate for nadh from the e.nadh complex is completely rate limiting when the group with a pk of 4 ... | 1989 | 2790001 |
| effects of antigen on tracheal circulation and smooth muscle in sheep of different ages. | the effects of ascaris suum antigen on tracheal circulation and tracheal smooth muscle tone were compared in two groups of sheep: the first group was 1 yr old (14 sheep) and the second 5 yr old (8 sheep). cranial tracheal arteries of anesthetized and paralyzed sheep were perfused at constant flow with monitoring of perfusion pressure. tracheal smooth muscle tone was assessed by measuring changes in the external diameter of the cranial trachea. close-arterial injection of antigen (1-20 micrograms ... | 1989 | 2793719 |
| tissue and ultrastructural localization of 5-hydroxytryptamine (serotonin) in the tissues of ascaris suum with energy dispersive x-ray spectrometry of immunoreactive structures. | muscle, hypodermis and gastrointestinal epithelial cells from adult female ascaris lumbricoides var. suum were found to contain serotonin based upon glyoxylic acid induced histofluorescence and indirect immunolabeling with an antiserotonin monoclonal antibody conjugated to protein a-colloidal gold. histofluorescence indicated that muscle-hypodermis and intestinal epithelial cells contained significant concentrations of 5-hydroxytryptamine while fluorescence was absent in the nerve cord and cutic ... | 1989 | 2807715 |
| prevalence of helminth infections in swine in denmark. | during 1982-1984 the occurrence of helminths in 66 sow herds was determined by examining faecal samples from swine of different age groups. ascaris suum was found in 88% of the herds, while oesophagostomum spp. and trichuris suis were found in 58 and 23% of the herds, respectively. strongyloides ransomi and hyostrongylus rubidus was not identified in any of the herds. the occurrence of both a. suum and oesophagostomum spp. was strongly related to the age of the animals. a. suum was most frequent ... | 1989 | 2815533 |
| cytotoxicity of mouse serum and peritoneal fluid for ascaris suum infective larvae. | serum from balb/c mice infected with ascaris suum resulted in 51cr release from a. suum infective larvae during in vitro incubation. peritoneal fluid from infected or noninfected balb/c mice also produced in vitro release of 51cr from a. suum larvae. 51cr release did not occur when anti-a. suum serum or peritoneal fluid from infected or noninfected mice were heat-inactivated. serum and peritoneal fluid from infected or noninfected akr-c5 deficient mice did not generate 51cr release from a. suum ... | 1989 | 2818799 |
| enhancement of leukotriene c4 release from primate airway macrophages by cellular interactions. | 1. cells were obtained from the lungs of macaque monkeys by bronchoalveolar lavage in order to study the role of cellular interactions in the release of leukotriene c4 (ltc4). 2. in normal monkeys, macrophages were the most abundant cell type recovered, whereas in monkeys sensitized with ascaris suum there was an increase in the numbers of eosinophils and mast cells recovered. 3. challenge of cells from both groups of animals with an optimal concentration of opsonized zymosan (opz) resulted in t ... | 1989 | 2819337 |
| the effect of nedocromil sodium and cromolyn sodium on antigen-induced responses in allergic sheep in vivo and in vitro. | we studied the effects of nedocromil sodium and cromolyn sodium on early and late bronchial responses to inhaled ascaris suum antigen in allergic sheep in vivo, and the antigen-induced contractile responses of sheep tracheal smooth muscle in vitro. for the in vivo studies the sheep were pretreated with aerosols of placebo (buffered saline solution), 20 mg of nedocromil sodium, or 20 mg of cromolyn sodium (both dissolved in 3 ml of buffered saline solution) and then challenged with aerosol antige ... | 1987 | 2822361 |
| identification of two different phosphofructokinase-phosphorylating protein kinases from ascaris suum muscle. | two different phosphofructokinase-phosphorylating protein kinases were separated from extracts of ascaris suum muscle by chromatography on deae-fractogel. they were tentatively designated phosphofructokinase kinase i and phosphofructokinase kinase ii. phosphofructokinase kinase i eluted from the chromatography column at an ionic strength of 0.07 and contained about 25% of the phosphofructokinase-phosphorylating activity assayed in crude extracts. the protein kinase activity was not stimulated by ... | 1988 | 2826470 |
| electron-transfer complexes of ascaris suum muscle mitochondria. iii. composition and fumarate reductase activity of complex ii. | complex ii of the anaerobic respiratory chain in ascaris muscle mitochondria showed a high fumarate reductase activity when reduced methyl viologen was used as the electron donor. the maximum activity was 49 mumol/min per mg protein, which is much higher than that of the mammalian counterpart. the mitochondria of ascaris-fertilized eggs, which require oxygen for its development, also showed fumarate reductase activity with a specific activity intermediate between those of adult ascaris and mamma ... | 1988 | 2843227 |
| cyclic nucleotides of lung lavage following acute antigen exposure in chronically exposed sheep. | in the sheep model of chronic allergic airway disease, we measured sequentially for 7 h following antigen exposure (ascaris suum) histamine and the cyclic nucleotides camp and cgmp in bronchoalveolar lavage (bal) fluids. results in the six sheep with chronic allergic airway disease (reactive sheep) were compared to those of three sheep chronically exposed to antigen but without acute or chronic airway response (nonreactive sheep). histamine levels in bal fluids were not modified after antigen ex ... | 1988 | 2843465 |
| esr studies on iron-sulfur clusters of complex ii in ascaris suum mitochondria which exhibits strong fumarate reductase activity. | complex ii of ascaris suum mitochondria, which functions as fumarate reductase in physiological conditions, contains three types of iron-sulfur clusters. these correspond to clusters s-1, s-2 and s-3 and are distinguishable by low-temperature esr studies. cluster s-1 is reduced by succinate, giving esr signals with gz, gy and gx values at 2.033, 1.939 and 1.920. the existence of cluster s-2 is suggested by an enhancement of the s-1 spin relaxation induced upon reduction of s-2 by dithionite. clu ... | 1988 | 2849561 |
| antigen challenge and canine tracheal mucus. | tracheal mucus hypersecretion was demonstrated in 7 dogs with cutaneous sensitivity to extracts of ascaris suum when the dogs were exposed to aerosols of the same antigen. there was a correlation between antigen skin sensitivity and the aerosol dose at which hypersecretion first occurred. in addition to hypersecretion, antigen challenge led to significant alterations in mucus viscoelasticity. the dose-response pattern was similar to that seen with methacholine and histamine aerosol administratio ... | 1985 | 2861166 |
| transcuticular solute movement in parasitic nematodes: relationship between non-polar solute transport and partition coefficient. | the rate constants (k) for steroid transport across the isolated cuticle of ascaris suum were, linearly related to partition coefficients (p) of steroids in model solvents. the slope of log p vs log k for an isolated cuticle was closest to unity for the more polar solvents, octanol and ether, suggesting that the barrier to steroid movement across the cuticle has partition properties similar to these solvents. the transcuticular movement of steroids into intact adult a. suum and infective larvae ... | 1986 | 2874926 |
| effects of sk&f 93944 (temelastine), a potent histamine h1-receptor antagonist in pharmacologic and antigen-induced bronchoconstriction. | sk&f 93944, a previously reported non-sedating histamine h1-receptor antagonist, was evaluated for its ability to block pharmacologic-and antigen-induced bronchoconstriction. in the isolated guinea pig trachea, sk&f 93944 (10(-9)-10(-7) m) produced a concentration-dependent inhibition of contractions produced by histamine (pkb = 9.5). another histamine antagonist, mepyramine (10(-8)-10(-6) m), was less potent (pkb = 8.5). sk&f 93944 (10(-8), 10(-7) m) also significantly depressed the rapid initi ... | 1986 | 2875234 |
| gamma-glutamyl transpeptidase activity in ascaris suum. | using histochemical techniques gamma-glutamyl transpeptidase activity has been localized mainly in the cuticle-hypodermis in ascaris suum. the specific activity of gamma-glutamyl transpeptidase was 3.87 +/- 0.49 mumol h-1 (g tissue)-1 in cuticle-hypodermis as compared to gastrointestinal tract, where it was 0.07 +/- 0.02 mumol h-1 (g tissue)-1, and muscle and reproductive tract where it was less than 0.001 mumol h-1 (g tissue)-1. when cuticle sections were incubated with labelled glutamine a lar ... | 1986 | 2876379 |
| correlation of myosin light chain phosphorylation and gamma aminobutyric acid receptors in ascaris suum muscle. | four different muscle relaxants were compared as to their effects on tonic ascaris suum muscle to: physiological activity, intracellular response to regulatory light chain phosphorylation, and receptor pharmacology. perfusion of ascaris suum muscle with each relaxant, gamma-aminobutyric acid, avermectin, piperazine and chlorpromazine resulted in a dose-dependent loss of muscle tone. comparison of intracellular effects indicated that gamma-aminobutyric acid and avermectin perfusion initiated an i ... | 1987 | 2885136 |
| ecdysteroids during embryonation of eggs of ascaris suum. | 1. the optimal temperature for in vitro development of fertilized eggs of ascaris suum was 24 degrees c. 2. samples (2 x 10(7) eggs) were obtained from in vitro embryonating cultures every 3 days for 4 weeks; lipids were extracted, partially purified, fractionated with hplc and analyzed for ecdysteroids by radioimmunoassay. 3. free ecdysone and 20-hydroxyecdysone (20-he) were at low levels (less than 20 pg) in freshly excised eggs and rose to maximal values on day 6 of embryonation. 4. conjugate ... | 1987 | 2887364 |
| serotonin (5-hydroxytryptamine) turnover in adult female ascaris suum tissue. | 1. the 5-hydroxytryptamine (5-ht, serotonin) turnover was examined in the tissues of adult female ascaris suum. the 5-ht turnover was highest in the intestine at 34.7 ng 5-ht produced/mg protein/hr and 13.8 ng 5-ht produced/mg protein/hr in muscle tissue. 2. the levels of 5-ht metabolites namely tryptophan, 5-hydroxytryptophan, 5-hydroxytryptamine, 5-hydroxyindole acetic acid and 5-hydroxytryptophol were measured in muscle and intestinal tissue of adult a. suum. 3. parachlorophenylalanine inhibi ... | 1988 | 2905947 |
| a unique cytoskeleton associated with crawling in the amoeboid sperm of the nematode, ascaris suum. | nematode sperm extend pseudopods and pull themselves over substrates. they lack an axoneme or the actin and myosins of other types of motile cells, but their pseudopods contain abundant major sperm protein (msp), a family of 14-kd polypeptides found exclusively in male gametes. using high voltage electron microscopy, a unique cytoskeleton was discovered in the pseudopod of in vitro-activated, crawling sperm of the pig intestinal nematode ascaris suum. it consists of 5-10-nm fuzzy fibers organize ... | 1989 | 2910878 |
| passive membrane properties of motorneurons and their role in long-distance signaling in the nematode ascaris. | in the motornervous system of the large parasitic nematode, ascaris suum, the dorsal and ventral nerve cords are connected by a repeating pattern of single identified motorneuron processes, called commissures (stretton et al., 1978). by making microelectrode penetrations of the commissures, we here report the first successful intracellular recordings of nematode neurons. these cells, like muscle cells of ascaris, exhibit resting potentials of approximately -30 to -40 mv. several tests indicate t ... | 1989 | 2918369 |
| alterations in ascaris suum larval burdens, eosinophil numbers, and lysophospholipase activity associated with low levels of dietary iron. | the effect of various amounts of dietary iron on the immune response was investigated using balb/canncr/br mice infected with ascaris suum. changes in numbers of larvae, numbers of eosinophils, and levels of lysophospholipase (lpl) activity in lung or liver tissues were analyzed from nonimmune and immunized mice at 2 and 7 days postinfection (pi). various iron diets did not influence the numbers of tissue larvae, eosinophils, or the lpl activity in lungs or livers of nonimmunized mice at various ... | 1989 | 2926596 |
| effect of calcium antagonist gallopamil on antigen-induced early and late bronchoconstrictor responses in allergic sheep. | calcium channel blockers have been shown to modify acute antigen-induced bronchoconstriction in allergic animals and in some human subjects with bronchial asthma; however, the effects of calcium antagonists on late-phase bronchial responses are not known. in this investigation, we studied the effects of calcium antagonist gallopamil on antigen-induced early- and late-phase bronchial responses in sheep allergic to ascaris suum antigen. in 11 unsedated sheep, specific lung resistance (srl) was mea ... | 1989 | 2930069 |
| survey of internal parasites in western australian pig herds. 1. prevalence. | between september 1982 and march 1984, 101 western australian piggeries with 15 or more sows were surveyed to determine the prevalence of internal parasites and examine the relationship between parasitism and management practices. faecal samples were collected from 20 pigs in 4 age groups in randomly selected piggeries, and examined for the presence of eggs of helminth parasites and protozoan cysts. evidence of nematode parasites was found in 79% of piggeries. sows were more commonly affected th ... | 1989 | 2930392 |
| fc and c3b receptor changes on mouse peritoneal leucocytes following stimulation with an extract of ascaris suum. | changes in igg-fc and igm-c3b receptors of mouse peritoneal leucocytes after in vivo stimulation with ascaris suum adult extract were studied. erythrocyte igg-fc rosettes were found to initially be decreased with a. suum stimulated neutrophils, eosinophils and macrophages in comparison with nonstimulated cells. igg-fc rosettes then increased with time and remained high for a 60 min exposure time. igm-c3b rosette formation was lower with a. suum stimulated leucocytes throughout 60 min of exposure ... | 1985 | 2932876 |
| properties and localization of chitin synthase in ascaris suum eggs. | investigations were carried out into the properties and localization of chitin synthase (ec 2.4.1.16) in connection with the formation of ascaris suum egg-shells. the chitin synthase of eggs exhibited properties very similar to those found in other chitin-synthesizing organisms. it was stimulated by the presence of atp, glcnac and mg2+ with maximal activity at ph 7.0. in the phase of active chitin synthesis, during formation of the primary shell layers, chitin synthase was bound to the structure ... | 1986 | 2938060 |
| protective immunity to ascaris suum: analysis of swine peripheral blood cell subsets using monoclonal antibodies and flow cytometry. | outbred domestic swine or sla inbred miniature swine were exposed to ascaris suum either naturally on contaminated lots or by inoculation with uv-irradiated attenuated eggs. both inbred and outbred swine developed virtually complete protection to a challenge of 10 000 eggs after natural exposure, but inbred swine were less resistant than outbred swine after uv-egg exposure. flow cytometric analysis of peripheral blood mononuclear cells from these animals, performed to determine changes in cell s ... | 1986 | 2939618 |
| experimental larval ascaridosis (ascaris suum goetze, 1782) in white mice. i. histochemical studies on the liver. | | 1985 | 2940825 |
| correlation between hysteresis and allosteric properties for phosphofructokinase from ascaris suum. | the ascaris suum phosphofructokinase exhibits hysteretic transitions in the time course for fructose 6-phosphate (f6p) phosphorylation in addition to allosteric properties when assayed at ph values below 8. conditions that enhance hysteretic changes also enhance cooperative interactions and thus there appears to be a link between hysteresis and cooperativity. initiation of reaction with either f6p or phosphofructokinase results in a pronounced lag, while initiation of the reaction with mgatp res ... | 1987 | 2958457 |
| reaction of ascaris suum phosphofructokinase with diethylpyrocarbonate. inactivation and desensitization to allosteric modulation. | reaction of the phosphofructokinase from ascaris suum with the reagent, diethylpyrocarbonate (depc), results in the loss of enzymatic activity. treatment of the inactivated enzyme with hydroxylamine brings about the recovery of almost 80% of the original activity suggesting that the modified residues are histidines. further evidence for the modification of histidines is that concomitant with the loss of activity, there is a change in a242 nm that corresponds to the derivatization of 5-6 histidin ... | 1987 | 2958458 |
| kinetic mechanism of ascaris suum phosphofructokinase desensitized to allosteric modulation by diethylpyrocarbonate modification. | the kinetic mechanism of phosphofructokinase has been determined at ph 8 for native enzyme and ph 6.8 for an enzyme desensitized to allosteric modulation by diethylpyrocarbonate modification. in both cases, the mechanism is predominantly steady state ordered with mgatp binding first in the direction of fructose 6-phosphate (f6p) phosphorylation and rapid equilibrium random in the direction of mgadp phosphorylation. this is a unique kinetic mechanism for a phosphofructokinase. product inhibition ... | 1987 | 2958459 |
| losses to internal parasites in swine production. | experimental infections of pigs with strongyloides ransomi, stephanurus dentatus, ascaris suum, oesophagostomum spp. or trichuris suis at increasing levels generally decrease daily gain and increase feed to gain ratio linearly. at lower, subclinical levels of infection, the feed to gain ratio typically is increased 3% to 6% by an infection. based on low-level experimental infections with a. suum, the economic loss to producers in the u.s. from increased feed to gain ratio is estimated at $155 mi ... | 1988 | 2969380 |
| dolichol kinase in ascaris suum and onchocerca volvulus. | dolichol kinase was demonstrated in the microsomal fraction of ascaris suum and onchocerca volvulus. the enzyme from nematodes exhibited specificity for ctp as phosphoryl donor and was found to be inhibited by the reaction product cdp. enzyme activity was optimal at ph 7.4, in the temperature range between 30 degrees and 37 degrees c, and in the presence of 0.5% triton x-100. in addition, the enzyme was found to depend on divalent cations for activity; mg2+ being more effective than mn2+ and ca2 ... | 1985 | 2985982 |
| suppression of pulmonary and systemic vascular histamine h2-receptors in allergic sheep. | we have previously demonstrated a depression of airway h2-receptor function in sheep allergic to ascaris suum antigen. to investigate whether this is a generalized defect, we studied the h1- and h2- histamine receptor functions in the pulmonary and systemic circulations of allergic and nonallergic sheep. pulmonary arterial pressure, and cardiac output were measured for calculation of pulmonary vascular resistance (pvr) and systemic vascular resistance (svr) before and immediately after a rapid i ... | 1986 | 3007427 |
| structural analysis of the calcium-binding protein calmodulin from ascaris suum obliquely striated muscle. | calmodulin was purified from the obliquely striated skeletal muscle of ascaris suum. the calmodulin had a molecular weight of 16,400 and the amino acid composition indicated it is highly similar to other purified calmodulins, showing insignificant variation in 12 of 17 residues. in the residues that showed variation, a trend towards conservative substitution was observed. spectrophotometric absorption maxima of 276 nm and 283 nm were observed. a molar absorption coefficient of 7,800 was calculat ... | 1986 | 3016225 |
| prevention of canine anaphylaxis with isoproterenol. | sympathomimetic agents are used to reverse the cardiovascular and respiratory abnormalities that are associated with anaphylactic shock. in addition, in vitro studies have demonstrated that beta-adrenergic stimulation modulates ige-mediated release of chemical mediators. to separately evaluate the ability of isoproterenol, a beta-adrenergic agonist, to prevent mediator release and anaphylactic shock, as well as to reverse the cardiopulmonary manifestations of systemic anaphylaxis, we administere ... | 1986 | 3017167 |
| reversible activation and inactivation of phosphofructokinase from ascaris suum by the action of tissue-homologous protein phosphorylating and dephosphorylating enzymes. | in the presence of atp-mg2+, purified phosphofructokinase from ascaris suum muscle was effectively phosphorylated and activated in vitro by a protein kinase purified from the same tissue. both effects were reversed by the action of a purified protein phosphatase from the same tissue. the findings suggest the presence of a highly potent interconversion mechanism for phosphofructokinase in the muscle of the parasitic nematode. | 1986 | 3021124 |
| monkeys infected with ascaris suum (a new in vivo model of airway disease): protective effect of nedocromil sodium and sodium cromoglycate against bronchial antigen challenge. | the experimental infection of macaca arctoides monkeys with the nematode parasite ascaris suum induces an ige-mediated immediate hypersensitivity reaction similar to that seen in naturally sensitive animals. in the present study, the effects of inhalation of ascaris suum antigen on lung function in infected monkeys were investigated, together with the effects of aerosolized nedocromil sodium and sodium cromoglycate (each 4 mg) given 5 min prior to antigen challenge. ascaris suum-infected macaca ... | 1986 | 3021491 |
| ascaris suum phosphofructokinase. phosphorylation by protein kinase and sequence of the phosphopeptide. | phosphorylation of the ascarid phosphofructokinase with the catalytic subunit of beef heart cyclic amp-dependent protein kinase results in the incorporation of 1 mol of p/mol of subunit. accompanying the phosphorylation there is a 3-4-fold increase in catalytic activity when measured at ph 6.8 with inhibitory levels of atp. studies on the effect of phosphorylation on the atp saturation curve demonstrated that phosphorylation decreased the inhibitory action of atp. the apparent km of the catalyti ... | 1987 | 3025208 |
| biochemical changes during the aerobic-anaerobic transition in ascaris suum larvae. | ascaris suum l3 larvae isolated from rabbit lungs undergo the third ecdysis to l4 larvae after 3 days in culture under a gas phase of 85% n2/10% co2/5% o2. the l3 larvae contain substantial malic enzyme activity and are capable of producing small amounts of the reduced organic acids characteristic of the fermentative pathways which operate in the adult. however, only a small portion of the total carbon utilized is accounted for by these reduced acids and their motility is cyanide-sensitive, sugg ... | 1987 | 3033496 |
| ascaris suum: partial isolation and characterization of hypodermis from the adult female. | a method was developed to remove the muscle from body wall strips of adult female ascaris suum resulting in a hypodermis cuticle preparation. optimum treatment for obtaining the hypodermis cuticle was a 15 min incubation with trypsin (2.0 mg/ml) at room temperature, followed by mechanical removal of the muscle. the hypodermis cuticle prepared in this manner incorporated radiolabeled amino acids into cuticular and hypodermal proteins; incorporation was inhibited by protein synthesis inhibitors. c ... | 1987 | 3034657 |
| efficacy of piperazine dihydrochloride against ascaris suum and oesophagostomum species in naturally infected pigs. | a controlled trial was performed to evaluate the efficacy of piperazine dihydrochloride in a new granular formulation (ascarex d) against naturally occurring infections with ascaris suum, oesophagostomum dentatum and o quadrispinulatum. treatment effects were estimated on the basis of parasites recoverable from the intestinal contents. given orally at 200 mg per kg body weight the compound showed an efficacy of 99 to 100 per cent against a suum and the nodular worms. egg excretion of the respect ... | 1988 | 3046112 |
| immunocytochemical demonstration of a neuropeptide in ascaris suum (nematoda) using an antiserum to fmrfamide. | a fmrfamide-like peptide has been detected in the nematode ascaris suum, using the peroxidase-anti-peroxidase (pap) immunocytochemical technique. positive reactions were obtained in both the central nervous system and the peripheral nervous system of the worm, the strongest reactions being in the anterior nerve ring, the cephalic papillary ganglia, the lateral ganglia and the dorso-rectal ganglion. immunoreactivity was observed along the length of the main nerve cords of the worm and, to a lesse ... | 1988 | 3050818 |
| the secreted and somatic antigens of the third stage larva of anisakis simplex, and antigenic relationship with ascaris suum, ascaris lumbricoides, and toxocara canis. | the in vitro-released 'excretory/secretory' (es) and somatic antigens of the third stage (infective) larva of anisakis simplex were characterised by radioiodination, immunoprecipitation, and sodium dodecyl sulphate-polyacrylamide gel electrophoresis. oral infection with the parasite elicited a substantial igg antibody response to es in infected rabbits and humans, with a minimal response to somatic materials. serial serum sampling in experimental infection showed that there was a sequential reco ... | 1988 | 3054540 |
| murine delayed type hypersensitivity is suppressed by ascaris suum extract. | we studied the suppressive effect of an ascaris suum extract on delayed-type hypersensitivity (dth) reactions to ovalbumin (ova) and to bacillus calmette-guerin (bcg). the ability of mice to develop dth reactions to both antigens was suppressed when an immunizing dose of the antigen was given subcutaneously together with the ascaris extract. partial or complete suppression of the response to ova was obtained by the use of 400 or 1000 micrograms of ascaris extract, respectively. the response to b ... | 1988 | 3067811 |
| the polyamine metabolism of filarial worms as chemotherapeutic target. | parasite-specific putrescine-n-acetyltransferase and polyamine oxidase, both involved in the reversed pathway of polyamine metabolism, were demonstrated for ascaris suum and onchocerca volvulus. berenil-treatment was found to be correlated with accumulation of polyamines, especially spermine, obviously due to blockaded polyamine interconversion. furthermore it was shown that added spermine to the culture medium led to the death of worms. these specificities might be exploited for chemotherapy of ... | 1988 | 3076347 |
| ascaris suum: immunoperoxidase and fluorescent probe analysis of host proteases and parasite proteinase inhibitors in developing eggs and second stage larvae. | live ascaris suum females were incubated in medium containing chymotrypsin liganded to fluorescein-5-isothiocyanate, and eggs in the parasite's genital tract took up the probe and fluoresced. eggs passed by these worms into the medium containing fluorescent probe retained their fluorescence in formaldehyde-saline and by 65 days had developed into second stage infective larvae. eggs passed naturally by untreated worms were incubated in media containing fluorescent probes and all of the eggs expos ... | 1986 | 3082662 |
| comparative histochemical studies of glycosidase activity in some helminths. | comparative histochemical studies of glycosidase activity were carried out in clonorchis sinensis, eurytrema pancreaticum, fasciola hepatica, dipylidium caninum, hymenolepis nana, ascaris suum, toxocara canis, ancylostoma caninum, trichuris vulpis and dirofilaria immitis. the enzymes examined were: n-acetyl-beta-glucosaminidase (ec 3.2.1.30), beta-glucuronidase (ec 3.2.1.31) and beta-galactosidase (ec 3.2.1.23). there were variations in enzyme distribution and intensity among the species and als ... | 1986 | 3084622 |
| indomethacin and fpl-57231 inhibit antigen-induced airway hyperresponsiveness in sheep. | we compared the development of antigen-induced airway hyperresponsiveness (ahr) 24 h after challenge with ascaris suum antigen in allergic sheep with acute (n = 7) and with dual (n = 7) airway responses and then attempted to modify this ahr. cholinergic airway responsiveness was determined by measuring the carbachol dose required to increase specific lung resistance (srl) 150% (i.e., pc150). subsequently the sheep were challenged with antigen and srl was measured at predetermined times to docume ... | 1986 | 3093455 |
| quaternary structure of erythrocruorin from the nematode ascaris suum. evidence for unsaturated haem-binding sites. | the quaternary structure of erythrocruorin from the nematode ascaris suum was studied. the native protein had a sedimentation coefficient, at a protein concentration of 1 mg/ml, of 11.6 +/- 0.3 s and an mr, as determined by sedimentation equilibrium, of 332,000 +/- 17,000. sds/polyacrylamide-gel electrophoresis gave one band with a mobility corresponding to an mr of 43,000 +/- 2000. the mr of the polypeptide chain was determined to be 41,600 +/- 1,500 by sedimentation equilibrium in 6 m-guanidin ... | 1987 | 3109399 |
| biochemical and immunological systematics of some ascaridoid nematodes: genetic divergence between congeners. | vertical starch gel electrophoresis and trefoil immunodiffusion were used to study the systematics of some ascaridoid nematodes. within the ascarididae, the time scale of divergence was too great for intergeneric electrophoretic comparisons. congeneric electrophoretic comparisons of baylisascaris procyonis (host--raccoon) versus baylisascaris transfuga (host--black bear), and toxocara canis (host--domestic dog) versus toxocara cati (host--domestic cat) yielded nei genetic distance coefficients o ... | 1987 | 3114467 |
| a major role for phospholipase a2 in antigen-induced arachidonic acid release in rat mast cells. | cross-linking of ige receptors by antigen stimulation leads to histamine release and arachidonic acid release in rat peritoneal mast cells. investigators have reported a diverse distribution of [3h]arachidonate that is dependent on labelling conditions. mast cells from rat peritoneal cavity were labelled with [3h]arachidonic acid for different periods of time at either 30 or 37 degrees c. optimum labelling was found to be after 4 h incubation with [3h]arachidonate at 30 degrees c, as judged by c ... | 1987 | 3120703 |