| curcumin reduces oxidative and nitrative dna damage through balancing of oxidant-antioxidant status in hamsters infected with opisthorchis viverrini. | opisthorchis viverrini (ov) infection is endemic in northeastern thailand. we have previously reported that ov infection induces oxidative and nitrative dna damage via chronic inflammation, which contributes to the disease and cholangiocarcinogenesis. here, we examined the effect of curcumin, an antioxidant, on pathogenesis in ov-infected hamsters. dna lesions were detected by double immunofluorescence and the hepatic expression of oxidant-generating and antioxidant genes was assessed by quantit ... | 2009 | 19753608 |
| a novel nuclear marker, pm-int9, for phylogenetic studies of opisthorchis felineus, opisthorchis viverrini, and clonorchis sinensis (opisthorchiidae, trematoda). | opisthorchis felineus, o. viverrini, and clonorchis sinensis, the trematodes of the family opisthorchiidae, are important human parasites. two previous studies (kang et al. parasitol int 57:191-197, 2008; katokhin et al. dokl biochem biophys 421:214-217, 2008) have provided evidence using ribosomal and mitochondrial sequences that o. viverrini, o. felineus, and c. sinensis are closely related. we developed a novel nuclear marker, pm-int9, which included the ninth intron of the paramyosin gene an ... | 2009 | 19777262 |
| a granulin-like growth factor secreted by the carcinogenic liver fluke, opisthorchis viverrini, promotes proliferation of host cells. | the human liver fluke, opisthorchis viverrini, infects millions of people throughout south-east asia and is a major cause of cholangiocarcinoma, or cancer of the bile ducts. the mechanisms by which chronic infection with o. viverrini results in cholangiocarcinogenesis are multi-factorial, but one such mechanism is the secretion of parasite proteins with mitogenic properties into the bile ducts, driving cell proliferation and creating a tumorigenic environment. using a proteomic approach, we iden ... | 2009 | 19816559 |
| helminths and malignancy. | it has been estimated that chronic infections with viruses, bacteria and parasites contribute to 17.8% of the global burden of cancer, although only a relatively small proportion of the infection-related cancers can be attributed to helminth infections. these are important because of the high number of people who are exposed or infected worldwide. carcinogenesis associated with helminth infections is a complex process, which may involve several different mechanisms, but chronic inflammation is a ... | 2009 | 19825108 |
| rapid molecular detection of opisthorchis viverrini in human fecal samples by real-time polymerase chain reaction. | real-time fluorescence resonance energy transfer (fret) polymerase chain reaction (pcr) supplemented with melting curve analysis is a highly sensitive and fast method offering a high throughput. we report the development of a real-time fret pcr for molecular detection of opisthorchis viverrini in human fecal samples. the diagnostic sensitivity, specificity, accuracy, and positive and negative predictive values of this method were 97.5%, 100%, 98.9%, 100%, and 98.2%, respectively. the sensitivity ... | 2009 | 19861632 |
| microsatellite loci in the carcinogenic liver fluke, opisthorchis viverrini and their application as population genetic markers. | opisthorchis viverrini is a carcinogenic foodborne trematode endemic in southeast asia especially in thailand and the lao people's democratic republic. opisthorchiasis causes hepatobiliary diseases and cholangiocarcinoma (bile duct cancer). currently there is substantial evidence on genetic variation of o. viverrini but the information on population genetic structure is lacking. because microsatellite dna of this parasite is not available, we for the first time isolated and utilized microsatelli ... | 2010 | 19900579 |
| formation of 8-nitroguanine, a nitrative dna lesion, in inflammation-related carcinogenesis and its significance. | chronic infection and inflammation contribute to a substantial part of environmental carcinogenesis. recently, it has been estimated that chronic inflammation accounts for approximately 25% of cancer cases. various infectious diseases and physical, chemical, and immunological factors participate in inflammation-related carcinogenesis. under inflammatory conditions, reactive oxygen and nitrogen species, which are generated from inflammatory and epithelial cells, may play an important role in carc ... | 2009 | 19921494 |
| elucidating the transcriptome of fasciola hepatica - a key to fundamental and biotechnological discoveries for a neglected parasite. | liver flukes of animals are parasitic flatworms (platyhelminthes: digenea) of major socioeconomic importance in many countries. key representatives, such as fasciola hepatica and f. gigantica, cause "liver fluke disease" (= fascioliasis), which is of major animal health significance worldwide. in particular, f. hepatica is a leading cause of production losses to the livestock (mainly sheep and cattle) and meat industries due to clinical disease, reduced weight gain and milk production, and death ... | 2010 | 20006979 |
| myristoylated alanine-rich c kinase substrate phosphorylation promotes cholangiocarcinoma cell migration and metastasis via the protein kinase c-dependent pathway. | myristoylated alanine-rich c kinase substrate (marcks), a substrate of protein kinase c (pkc) has been suggested to be implicated in cell adhesion, secretion, and motility through the regulation of the actin cytoskeletal structure. the quantitative real-time-polymerase chain reaction analysis revealed that marcks is significantly overexpressed in opisthorchis viverrini-associated cholangiocarcinoma (cca) (p = 0.001) in a hamster model, which correlated with the results of mrna in situ hybridizat ... | 2010 | 20047593 |
| the secreted and surface proteomes of the adult stage of the carcinogenic human liver fluke opisthorchis viverrini. | infection with the human liver fluke, opisthorchis viverrini, is a serious public health problem in thailand, laos and nearby locations in southeast asia. both experimental and epidemiological evidence strongly implicate liver fluke infection in the etiology of one of the liver cancer subtypes, cholangiocarcinoma (cca). to identify parasite proteins critical for liver fluke survival and the etiology of cca, offgel electrophoresis and multiple reaction monitoring were employed to characterize 300 ... | 2010 | 20049860 |
| lipid peroxidation and etheno dna adducts in white blood cells of liver fluke-infected patients: protection by plasma alpha-tocopherol and praziquantel. | chronic infection by the liver fluke opisthorchis viverrini is a strong risk factor for cholangiocarcinoma. to clarify the involvement of oxidative stress and lipid peroxidation-derived dna damage, etheno (epsilon)-dna adducts (epsilonda, epsilondc) in wbc and plasma alpha-tocopherol were measured in samples collected from o. viverrini-infected thai patients (n = 50) and healthy noninfected volunteers (n = 20). epsilonda and epsilondc levels were three to five times higher (p < 0.001) in infecte ... | 2010 | 20056652 |
| secreted cysteine proteases of the carcinogenic liver fluke, opisthorchis viverrini: regulation of cathepsin f activation by autocatalysis and trans-processing by cathepsin b. | opisthorchis viverrini is an important helminth pathogen of humans that is endemic in thailand and laos. adult flukes reside within host bile ducts and feed on epithelial tissue and blood cells. chronic opisthorchiasis is associated with severe hepatobiliary diseases such as cholangiocarcinoma. here we report that adult o. viverrini secrete two major cysteine proteases: cathepsin f (ov-cf-1) and cathepsin b1 (ov-cb-1). ov-cf-1 is secreted as an inactive zymogen that autocatalytically processes a ... | 2010 | 20070308 |
| opisthorchis viverrini: evaluation of 28 kda glutathione s-transferase as diagnostic tool in human opisthorchiasis. | the liver fluke opisthorchis viverrini is the agent of human opisthorchiasis in thailand with a high prevalence observed in the rural population of north and northeastern regions of the country. a focus of research has therefore been the development of diagnostic tools to indicate infection by this parasite. in the present study, a 28 kda glutathione s-transferase of o. viverrini (ov28gst), which is found in the excretion/secretion product of the parasite, was evaluated for its application in di ... | 2010 | 20096257 |
| prevalence and potential risk factors of rhinitis and atopic eczema among schoolchildren in vientiane capital, lao pdr: isaac questionnaire. | in 1998, an epidemiological study on asthma and allergic diseases using isaac questionnaire in laos was first conducted in the recommended schools located in vientiane capital showing that the prevalence of rhinoconjunctivitis and atopic eczema were 23.7% and 7.1% among children aged 13-14 year-old, respectively. this study aimed to reassess the prevalence of rhinoconjunctivitis and atopic eczema using the same isaac questionnaire by employing random sampling method and to identify the potential ... | 2008 | 20103927 |
| involvement of mmp-9 in peribiliary fibrosis and cholangiocarcinogenesis via rac1-dependent dna damage in a hamster model. | peribiliary fibrosis caused by chronic infection with opisthorchis viverrini (ov) is a risk factor of cholangiocarcinoma (cca) in northeastern thailand. matrix metalloproteinases (mmps) are enzymes capable of degrading and remodeling the extracellular matrix in the process of fibrosis and carcinogenesis. we examined mmps expression and their role in fibrogenesis and cholangiocarcinogenesis in hamsters treated with ov and n-nitrosodimethylamine (ndma). we assessed the time profiles of mmps, induc ... | 2010 | 20162672 |
| monophyly of opisthorchis viverrini populations in the lower mekong basin, using mitochondrial dna nad1 gene as the marker. | the liver fluke, opisthorchis viverrini, causes serious public-health problems in the lower mekong basin. this study aimed to clarify whether o. viverrini populations may be genetically divided into sub-specific taxa. we collected 6 populations of o. viverrini from different places in cambodia, lao pdr, and thailand, along both sides of the mekong river, and analyzed the population structure of these using the mitochondrial nad1 gene as a marker. the results of the dna polymorphism measurements, ... | 2010 | 20197110 |
| cholangiocarcinoma: descriptive epidemiology and risk factors. | in theory, the term of cholangiocarcinoma is reserved for the tumours originating from the intrahepatic bile ducts. the problems of classification of the most frequent hilar tumours and the absence of histopathological confirmation in a large percentage of cases in cancer registries from many countries show the difficulty of establishing the specific epidemiologic behaviour of intrahepatic cholangiocarcinoma (icc). there are clearly two types of icc: the first one is the consequence of the recur ... | 2010 | 20202771 |
| effect of fingerroot on reducing inflammatory cells in hamster infected with opisthorchis viverrini and n-nitrosodimethylamine administration. | the active compound in fingerroot is effective in the treatment of many inflammatory diseases. the aim of our present study was to evaluate the efficacy of fingerroot on reducing histopathological changes in hamsters that were infected with the liver fluke opisthorchis viverrini or were administered n-nitrosodimethylamine (ndma), and then treated with fingerroot. light microscopic observation and liver function tests for alanine transaminase (alt), alkaline phosphatase (alp), and direct bilirubi ... | 2010 | 20306207 |
| food-borne trematode infections of humans in the united states of america. | this review examines the literature on imported (allochthonous) and local (autochthonous) cases of food-borne trematode (fbt) infections in the united states of america (usa) from 1890 to 2009. most of the literature is concerned with imported cases of the opisthorchiids clonorchis sinensis and opisthorchis viverrini. these flukes cause serious pathology in the liver and biliary system of humans. chronic cases may induce liver (hepatocarcinoma) and bile duct (cholangiocarcinoma) cancers in human ... | 2010 | 20352454 |
| reduction of periductal fibrosis in liver fluke-infected hamsters after long-term curcumin treatment. | chronic infection with the liver fluke, opisthorchis viverrini, induces advanced periductal fibrosis and is a relative risk factor for cholangiocarcinoma in southeastern asia. we examined the reducing effect of curcumin on hepatobiliary fibrosis using o. viverrini-infected hamsters supplemented with dietary 1% curcumin (w/w) as an animal model. the expression profile of matrix metalloproteinases (mmps) and tissue inhibitors of mmps (timps), cytokines, and collagens was assessed in relation to li ... | 2010 | 20420820 |
| detection of salivary antibodies to crude antigens of opisthorchis viverrini in opisthorchiasis and cholangiocarcinoma patients. | opisthorchis viverrini (o. viverrini; known as human liver fluke) is a major health problem in the northeastern region of thailand. infection with o. viverrini is the cause of hepatobiliary disease and cholangiocarcinoma (cca). previous studies demonstrated specific antibodies to crude o. viverrini antigens in serum from o. viverrini-infected patients. however, no studies have measured specific antibodies to o. viverrini antigens in saliva from patients with opisthorchiasis and cca. the objectiv ... | 2010 | 20446100 |
| up-regulation of annexin a2 in cholangiocarcinoma caused by opisthorchis viverrini and its implication as a prognostic marker. | cholangiocarcinoma (cca), or cancer of the bile ducts, is primarily associated with infection with the liver fluke opisthorchis viverrini in northeast thailand. the disease is associated with late presentation, poses challenges for diagnosis and has a high mortality rate--features that highlight the need for tumor markers. at present, there are no specific tumor markers that can indicate the early stages and status of cca. proteomic analysis of the proteins expressed on the surface of tumor cell ... | 2010 | 20493868 |
| proteomic identification of peroxiredoxin 6 for host defence against opisthorchis viverrini infection. | opisthorchis viverrini infection causes opisthorchiasis and is a risk factor for cholangiocarcinoma via chronic inflammation. to investigate the mechanism of o. viverrini -induced liver disease, we applied a proteomic approach to examine alterations in hepatic protein levels in o. viverrini -infected hamsters. two-dimensional gel electrophoresis (2de) revealed that o. viverrini infection induced upregulation (1.5- to 4.3-fold) of 25 proteins and downregulation (1.5 to 2.5-fold) of 24 proteins co ... | 2010 | 20500660 |
| chronic bacterial and parasitic infections and cancer: a review. | a relatively underestimated facet of infectious diseases is the association of chronic bacterial and parasitic infections with cancer development. therefore, we sought to evaluate the evidence regarding the association of such infections with the development of malignancy, excluding the overwhelming evidence of the association of helicobacter pylori and cancer. | 2010 | 20539059 |
| accumulation of miscoding etheno-dna adducts and highly expressed dna repair during liver fluke-induced cholangiocarcinogenesis in hamsters. | infection by opisthorchis viverrini, a risk factor for cholangiocarcinoma (cca) may act through chronic inflammation, oxidative stress and lipid peroxidation (lpo)-related damage and growth stimuli. 1,n6-etheno-2'-deoxyadenosine (epsilonda), and 3,n4-etheno-2'-deoxycytidine (epsilondc), markers for lpo-derived dna damage were highly increased in white blood cell and urine of o. viverrini-infected thai patients. in order to investigate tissue specificity etheno adducts were measured in a cholangi ... | 2010 | 20541562 |
| effect of curcumin on pathogenesis of hamster-opisthorchiasis through apoptosis-related gene expression. | the present study investigated the effect of curcumin, a phenolic compound with yellow color from curcuma longa l., on the expression of the apoptosis-related genes [bax (bcl-2 associated protein x), pkb, p53, mdm2 (mouse double minute 2), caspase 9, c-ski, smad1 and smad4] in hamster opisthorchiasis. on opisthorchis viverrini infection treated with dietary curcumin apoptosis-related gene expression profiles were similar to o. viverrini-infected group, but the expression levels seemed lower. lig ... | 2009 | 20578454 |
| copro-dna diagnosis of opisthorchis viverrini and haplorchis taichui infection in an endemic area of lao pdr. | the utility of differential copro-dna diagnosis using modified sample preparation steps of small liver and minute intestinal fluke infections was tested. fecal samples containing parasite eggs were washed extensively with diluted detergent solution. parasite eggs were concentrated by sedimentation and broken by microwaving before dna extraction. pcr targeting its1 and its2 regions were performed using primer specific for opisthorchis viverrini, haplorchis taichui and other related species. of 12 ... | 2010 | 20578479 |
| prevalence of opisthorchis viverrini infection in the canine and feline hosts in three villages, khon kaen province, northeastern thailand. | to determine the current reservoir status and prevalence of opisthorchis viverrini infection in endemic areas, 78 dogs and 22 cats were sampled for fecal examination in 3 villages along the chi river in khon kaen province, northeastern thailand. sex, age, color, identifiable markings, subjective body condition scores (bcs) and diet were recorded and analyzed. fecal samples were evaluated using direct smear and modified formalin-ethyl acetate fecal assays. all parasites found were identified. in ... | 2010 | 20578480 |
| unlocking the transcriptomes of two carcinogenic parasites, clonorchis sinensis and opisthorchis viverrini. | the two parasitic trematodes, clonorchis sinensis and opisthorchis viverrini, have a major impact on the health of tens of millions of humans throughout asia. the greatest impact is through the malignant cancer ( = cholangiocarcinoma) that these parasites induce in chronically infected people. therefore, both c. sinensis and o. viverrini have been classified by the world health organization (who) as group 1 carcinogens. despite their impact, little is known about these parasites and their interp ... | 2010 | 20582164 |
| food-borne trematodiases in southeast asia epidemiology, pathology, clinical manifestation and control. | the food-borne trematodiases are an important group of neglected tropical diseases (ntds). over 40 million people are infected with food-borne trematodes and 750 million (>10% of the world's population) are at risk of these ntds. here, we review the life cycles, epidemiology, clinical manifestations, pathology and pathogenesis, diagnosis, treatment, and prevention and control of the major food-borne trematodiases in southeast asia. we focus particularly on opisthorchiasis caused by opisthorchis ... | 2010 | 20624536 |
| protective effect of melatonin against opisthorchis viverrini-induced oxidative and nitrosative dna damage and liver injury in hamsters. | the liver fluke, opisthorchis viverrini, is the risk factor of cholangiocarcinoma, which is a major health problem in northeastern thailand. production of reactive oxygen and nitrogen species during the host's response leads to oxidative and nitrosative stress contributing to carcinogenesis. we investigated the protective effect of melatonin against o. viverrini-induced oxidative and nitrosative stress and liver injury. hamsters were infected with o. viverrini followed by oral administration of ... | 2010 | 20626588 |
| opisthorchiasis and opisthorchis-associated cholangiocarcinoma in thailand and laos. | liver fluke infection caused by opisthorchis viverrini is a major public health problem in thailand and the lao people's democratic republic (lao pdr; laos). currently, more than 600 million people are at risk of infection with these fish-borne trematodes and/or their close relatives. opisthorchiasis has been studied extensively in thailand, where about 8 million people are infected with the liver fluke. here we review the pathogenesis, control and re-emergence of o. viverrini infection, in part ... | 2010 | 20655862 |
| helminths in human carcinogenesis. | this review examines the salient literature on selected helminths involved in carcinogenicity in humans and updates information in an earlier review on cancer and helminths by mayer and fried (2007, advances in parasitology 65, 239-296). the earlier review was concerned with various helminths, i.e., trematodes, cestodes, and nematodes, that are definitely implicated as being carcinogenic. this review examines only those helminths, all of which turn out to be trematodes, that are definitely impli ... | 2011 | 20667649 |
| progress on the transcriptomics of carcinogenic liver flukes of humans--unique biological and biotechnological prospects. | liver flukes, such as clonorchis sinensis and opisthorchis viverrini, are food-borne parasites that have a major impact on the health of humans and animals, particularly in asia. however, the impact of c. sinensis and o. viverrini, in particular, is exacerbated in that these parasites can induce a malignant, untreatable cancer (cholangiocarcinoma, cca) in chronically infected people. as a result, these flukes are classified as group 1 carcinogens. despite their substantial socio-economic importa ... | 2010 | 20673796 |
| update on the diagnosis and treatment of food-borne trematode infections. | more than 40 million people are affected by food-borne trematode infections. diagnosis is unsatisfactory and there are only two drugs available for treatment and control: praziquantel and triclabendazole. this review provides an update on recent developments in the diagnosis and treatment of food-borne trematodiases. | 2010 | 20683264 |
| indirect effect of a turmeric diet: enhanced bile duct proliferation in syrian hamsters with a combination of partial obstruction by opisthorchis viverrini infection and inflammation by n-nitrosodimethylamine administration. | the present study revealed the indirect effect of a turmeric (tur) diet on the histopathological changes and proliferating cell nuclear antigen staining in syrian hamsters with partial obstruction by liver fluke (opisthorchis viverrini) infection and inflammation by n-nitrosodimethylamine (ndma) administration. the result of the analysis of histopathological changes shows that a tur diet has an anti-inflammatory property in the case of a single condition of ndma administration or o. viverrini in ... | 2011 | 20821233 |
| curcumin decreases cholangiocarcinogenesis in hamsters by suppressing inflammation-mediated molecular events related to multistep carcinogenesis. | cholangiocarcinoma (cca) is a highly metastatic tumor linked to liver fluke infection and consumption of nitrosamine-contaminated foods and is a major health problem especially in south-eastern asia. in search for a suitable chemopreventive agents, we investigated the effect of curcumin, a traditional anti-inflammatory agent derived from turmeric (curcuma longa), on cca development in an animal model by infection with the liver fluke opisthorchis viverrini and administration of n-nitrosodimethyl ... | 2011 | 20824699 |
| involvement of c-ski oncoprotein in carcinogenesis of cholangiocacinoma induced by opisthorchis viverrini and n-nitrosodimethylamine. | opisthorchiasis is the major public health problem in the endemic areas of thailand and laos because opisthorchis viverrini infection causes serious hepatobiliary diseases including cca. the molecular mechanism of the cca carcinogenesis induced by the infection remains obscure. to reveal the potential genes and signaling pathways to involve in the carcinogenesis, the present study investigated the expression of c-ski, an oncogene, and two tgf-β signaling pathway relative genes, tgf-β and smad4, ... | 2011 | 20853076 |
| genetic relationships within the opisthorchis viverrini species complex with specific analysis of o. viverrini from savannakhet, lao pdr by multilocus enzyme electrophoresis. | recent systematic studies of opisthorchis viverrini based on multilocus enzyme electrophoresis (mee) have shown that there are at least five genetic groups and possibly two cryptic species occurring in thailand and lao pdr each associated with a specific wetland system. a study based on mitochondrial dna (mtdna) gene analyses of an o. viverrini population from savannakhet (sv, lao pdr) clustered with several isolates from thailand and lao pdr although they originated from different river wetland ... | 2010 | 20865431 |
| opisthorchis viverrini excretory/secretory products induce toll-like receptor 4 upregulation and production of interleukin 6 and 8 in cholangiocyte. | biliary tract infection with the group i carcinogenic liver fluke opisthorchis viverrini is associated with severe inflammation leading to cholangiocarcinoma--a major biliary cancer in southeast asia. however, mechanism(s) by which the liver fluke induces host mucosal immune/inflammatory responses is unclear. in the present study we address whether a normal immortalized human cholangiocyte cell line (h69 cells) recognizes and responds to o. viverrini excretory/secretory products (oves). expressi ... | 2010 | 20887801 |
| identification and characterization of micrornas in clonorchis sinensis of human health significance. | clonorchis sinensis is a zoonotic parasite causing clonorchiasis-associated human disease such as biliary calculi, cholecystitis, liver cirrhosis, and it is currently classified as carcinogenic to humans for cholangiocarcinoma. micrornas (mirnas) are non-coding, regulating small rna molecules which are essential for the complex life cycles of parasites and are involved in parasitic infections. to identify and characterize mirnas expressed in adult c. sinensis residing chronically in the biliary ... | 2010 | 20920166 |
| rapid identification and differentiation of fasciola hepatica and fasciola gigantica by a loop-mediated isothermal amplification (lamp) assay. | the present study developed and validated a species-specific loop-mediated isothermal amplification (lamp) assay for the rapid detection and discrimination of fasciola hepatica and fasciola gigantica. the lamp assay is inexpensive, easy to perform and shows rapid reaction, wherein the amplification can be obtained in 45 min under isothermal conditions of 61 °c or 62 °c by employing a set of four species-specific primer mixtures and results can be checked through naked-eye visualization. the opti ... | 2010 | 20933335 |
| molecular evidence of opisthorchis viverrini in infected bithyniid snails in the lao people's democratic republic by specific hybridization probe-based real-time fluorescence resonance energy transfer pcr method. | naturally occurring bithyniid snails, bithynia siamensis goniomphalos (prosobranchia: bithyniidae), and their intermediate hosts were sampled from khammouane province, the lao people's democratic republic, and the prevalence of the carcinogenic human liver fluke, opisthorchis viverrini, was examined. the presence of o. viverrini cercariae in snails was examined by cercarial shedding test and then confirmed by specific hybridization probe-based real-time fluorescence resonance energy transfer (fr ... | 2010 | 21069540 |
| iso-electricfocusing of bithynia snail antigens for igg- and igg(1-4)-elisa detection of human opisthorchiasis. | diagnosis of opisthorchiasis is confirmed by the presence of characteristic eggs and worms. however, misdiagnosis may occur in light infections, and also due to the morphological similarity of opisthorchid eggs to other species. a finding of specific immune mediators can help confirm infection. this study used indirect elisa to detect total igg and igg(1-4) with selected antigens of bithynia siamensis goniomphalos extract, which were derived by liquid-phase iso-electricfocusing (ife). antigens ( ... | 2010 | 21073055 |
| sensitive and rapid detection of paragonimus westermani infection in humans and animals by loop-mediated isothermal amplification (lamp). | in the present study, a loop-mediated isothermal amplification (lamp) assay was developed and validated for the detection of paragonimus westermani adults, metacercariae, and eggs in human and animal samples. the lamp amplification can be finished in 45 min under isothermal condition at 60°c by employing a set of four species-specific primer mixtures and the results can be checked by naked-eye visualization. no amplification products were detected with deoxyribunucleic acid (dna) of related trem ... | 2010 | 21107864 |
| efficacy and safety of mefloquine, artesunate, mefloquine-artesunate, tribendimidine, and praziquantel in patients with opisthorchis viverrini: a randomised, exploratory, open-label, phase 2 trial. | praziquantel is the only drug available for treatment of opisthorchis viverrini, although in-vivo studies point to activity of mefloquine, artesunate, and tribendimidine against this liver fluke. we aimed to assess the efficacy and safety of these drugs compared with that of praziquantel in patients with o viverrini infection. | 2010 | 21111681 |
| hepatitis viruses and risk of cholangiocarcinoma in northeast thailand. | liver cancer is the most common cancer in males in thailand and the third in females. a high incidence of cholangiocarcinoma (cca) is estimated in the northeast of thailand. chronic infection with opisthorchis viverrini (ov) is the major risk factor for development of cca. it has been demonstrated that hcv infection is a risk factor for cca in non - endemic area of ov infection. we examined the association of hbv and hcv and risk of cca in the northeast thailand. all cases of cca were recruited ... | 2010 | 21133611 |
| [research progress on the relationship between three kinds of liver fluke infections and cholangiocarcinoma]. | liver fluke disease caused by clonorchis sinensis, opisthorchis viverrini and o. felineus is a food-borne zoonotic parasitic disease and widely prevalent in asia. the definitive hosts including human beings get the infection by ingestion of raw or undercooked fish or shrimp infested with metacercariae. long-term or severe infection of the flukes can lead to dysfunction of the liver, such as cholelithiasis, cholecystitis, and so on. researches indicated that there is an etiology relation between ... | 2010 | 21137319 |
| characterization and localization of saposin-like protein-2 (sap-2) in fasciola gigantica. | fasciola gigantica saposin-like protein-2 (fgsap-2) belongs to a family of lipid-interacting proteins that are involved in the cytolysis of target cells. in this study, we have cloned and expressed fgsap-2 and produced the antibody against this recombinant protein. rabbit antiserum against rfgsap-2 reacted with a similar native protein in the whole body extracts of the 4-week-old juvenile and adult stage, as well as a protein in their excretion-secretion, but not in the tegument. in situ hybridi ... | 2010 | 21188603 |
| prevalence of the intestinal flukes haplorchis taichui and h. yokogawai in a mountainous area of phongsaly province, lao pdr. | phongsaly province, located in the northernmost area of lao pdr, was previously suggested to be endemic for the liver fluke opisthorchis viverrini infection. to confirm, or rule out, this suggestion, the phonxay village in the khoua district, phongsaly province, was selected for a survey. ten volunteers (8 men and 2 women aged 31-57 years) who consumed raw freshwater fish and had gastrointestinal troubles were treated with a single dose of praziquantel (40 mg/kg) and pyrantel pamoate (10 mg/kg) ... | 2010 | 21234239 |
| curcumin induces a nuclear factor-erythroid 2-related factor 2-driven response against oxidative and nitrative stress after praziquantel treatment in liver fluke-infected hamsters. | praziquantel has been used for the treatment of liver fluke infection, but an oxidative/nitrative stress may occur after a short-term treatment and participate in side effects. in an attempt to reduce the adverse effects, we administered curcumin, an anti-inflammatory agent, to opisthorchis viverrini-infected hamsters treated with praziquantel. at 12h after treatment, curcumin decreased eosinophil infiltration and increased mononuclear cell infiltration in parallel with nuclear factor-erythroid ... | 2011 | 21256849 |
| a novel serum carbohydrate marker on mucin 5ac: values for diagnostic and prognostic indicators for cholangiocarcinoma. | the incidence of cholangiocarcinoma (cca) is increasing globally. currently, there is no powerful marker for the diagnosis of cca, which has led to late diagnosis and poor patient outcome. this study was designed to establish a new monoclonal antibody (moab) for detecting a serum marker associated with cca. | 2011 | 21287531 |
| prevalence of intestinal parasitic infections in military personnel and military dogs, thailand. | to determine the prevalence of intestinal parasitic infections and risk factors among military personnel and military dogs at the military dog center, veterinary and remount department, royal thai army, thailand. | 2009 | 21302414 |
| infection with the carcinogenic human liver fluke, opisthorchis viverrini. | throughout southeast asia there is a strikingly high incidence of cholangiocarcinoma (cca--hepatic cancer of the bile duct epithelium), particularly in people from rural settings in laos and northeast thailand who are infected with the liver fluke, opisthorchis viverrini, one of only three carcinogenic eukaryotic pathogens. more ubiquitous carcinogenic microbes, such as helicobacter pylori, induce cancer in less than 1% of infected people, while as many as one-sixth of people with opisthorchiasi ... | 2011 | 21311794 |
| opisthorchis viverrini infection causes liver and biliary cirrhosis in gerbils. | opisthorchis viverrini infection causes many hepatobiliary diseases, including cholangiocarcinoma. hence, the study of o. viverrini infection in humans is subject to ethical limitations, so an animal model, the syrian hamster, is often used. o. viverrini can develop into the adult stage not only in syrian hamsters but also in other animals, including gerbils, but until now, there has been no report on pathology and susceptibility in gerbils. the present study revealed the pathology of o. viverri ... | 2011 | 21340565 |
| genetic markers for studies on the systematics and population genetics of snails, bithynia spp., the first intermediate hosts of opisthorchis viverrini in thailand. | snails are the critical amplifying hosts of the liver fluke opisthorchis viverrini, the causative agent of hepatobiliary disease and cholangiocarcinoma in the mekong area of southeast asia. bithynia funiculata, b. siamensis goniomphalos and b. s. siamensis are the first intermediate hosts of o. viverrini in thailand. morphological similarity between bithynia species and subspecies creates problems for their taxonomic identification and an understanding of bithynia systematics. in this study, mul ... | 2011 | 21352793 |
| candidate genes involving in tumorigenesis of cholangiocarcinoma induced by opisthorchis viverrini infection. | opisthorchiasis-associated cholangiocarcinoma (cca) is one of main public health problems in opisthorchis viverrini endemic areas. although the definite relationship between prevalence of cca and the parasite infection has been demonstrated, the molecular mechanism of tumorigenesis is still unknown. in the present study, by using animal model of opisthorchiasis-associated cca, a kinetic analysis of cdna microarray was performed to screen the candidate genes that involve in the development of opi ... | 2011 | 21380578 |
| helminth and intestinal protozoa infections, multiparasitism and risk factors in champasack province, lao people's democratic republic. | detailed investigations of multiparasitism are scarce in the mekong river basin. we assessed helminth (trematode, nematode, and cestode), and intestinal protozoa infections, and multiparasitism in random population samples from three different eco-epidemiological settings in champasack province, southern lao people's democratic republic (lao pdr), and determined underlying risk factors. | 2011 | 21532735 |
| rapid detection of opisthorchis viverrini and strongyloides stercoralis in human fecal samples using a duplex real-time pcr and melting curve analysis. | human opisthorchiasis caused by the liver fluke opisthorchis viverrini is an endemic disease in southeast asian countries including the lao people's democratic republic, cambodia, vietnam, and thailand. infection with the soil-transmitted roundworm strongyloides stercoralis is an important problem worldwide. in some areas, both parasitic infections are reported as co-infections. a duplex real-time fluorescence resonance energy transfer (fret) pcr merged with melting curve analysis was developed ... | 2011 | 21537984 |
| rna interference targeting cathepsin b of the carcinogenic liver fluke, opisthorchis viverrini. | functional genomics have not been reported for opisthorchis viverrini or the related fish-borne fluke, clonorchis sinensis. here we describe the introduction by square wave electroporation of cy3-labeled small rna into adult o. viverrini worms. adult flukes were subjected to square wave electroporation employing a single pulse for 20ms of 125v in the presence of 50μg/ml of cy3-sirna. the parasites tolerated this manipulation and, at 24 and 48h after electroporation, fluorescence from the cy3-sir ... | 2011 | 21565281 |
| effect of soil surface salt on the density and distribution of the snail bithynia siamensis goniomphalos in northeast thailand. | opisthorchis viverrini infection is associated with human cholangiocarcinoma and northeast thailand has the highest incidence of this disease in the world. bithynia siamensis goniomphalos is the major freshwater snail intermediate host of o. viverrini in this area and an analysis based on geographical information systems was used to determine the effect of variation in soil surface salt on the density and distribution of this snail. a malacological survey was carried out in 56 water bodies in th ... | 2011 | 21590668 |
| risk factors for gallbladder cancer and cholangiocarcinoma: similarities, differences and updates. | introduction: far-ranging variation in the incidence of gallbladder cancer (gbc) and cholangiocarcinoma (cca) in different geographic regions on the globe may reflect the risk factor distribution for these tumors methods: the authors give a comprehensive review on the known risk factors for gbc and cca, and analyze both similarities and differences between the risk factors for the two main types of biliary cancer discussion and conclusion: leading risk factors for gbc include gallstones, female ... | 2011 | 21597894 |
| liver flukes: the malady neglected. | liver fluke disease is a chronic parasitic inflammatory disease of the bile ducts. infection occurs through ingestion of fluke-infested, fresh-water raw fish. the most well-known species that cause human infection are clonorchis sinensis, opisthorchis viverrini and opisthorchis felineus. adult flukes settle in the small intrahepatic bile ducts and then they live there for 20-30 years. the long-lived flukes cause long-lasting chronic inflammation of the bile ducts and this produces epithelial hyp ... | 2011 | 21603286 |
| sequences and gene organization of the mitochondrial genomes of the liver flukes opisthorchis viverrini and clonorchis sinensis (trematoda). | opisthorchis viverrini and clonorchis sinensis are important trematodes infecting humans and animals, belonging to the family opisthorchiidae. in the present study, we sequenced the nearly complete mitochondrial (mt) dna (mtdna) sequences of o. viverrini from laos, obtained the complete mtdna sequences of c. sinensis from china and korea, and revealed their gene annotations and genome organizations. the mtdna sequences of o. viverrini, c. sinensis (china isolate), c. sinensis (korea isolate) wer ... | 2011 | 21626421 |
| molecular identification of clonorchis sinensis and discrimination with other opisthorchid liver fluke species using multiple ligation-depended probe amplification (mlpa). | abstract: background: infections with the opisthorchid liver flukes clonorchis sinensis, opisthorchis viverrini, and o. felineus cause severe health problems globally, particularly in southeast asia. early identification of the infection is essential to provide timely and appropriate chemotherapy to patients. results: in this study we evaluate a pcr-based molecular identification method, multiplex ligation-dependent probe amplification (mlpa), which allows rapid and specific detection of single ... | 2011 | 21649899 |
| organization of the nervous system in opisthorchis viverrini investigated by histochemical and immunohistochemical study. | the structure and organization of the nervous system has been documented for various helminth parasites. however, the neuroanatomy of the carcinogenic liver fluke, opisthorchis viverrini has not been described. this study therefore investigated the organization of the nervous system of this fluke using cholinesterase activity, aminergic and peptidergic (fmrfamide-like peptides) immunostaining to tag major neural elements. the nervous system, as detected by acetylcholinesterase (ache) reaction, w ... | 2011 | 21807116 |
| distribution of repetitive dna sequences in chromosomes of five opisthorchid species (trematoda, opisthorchiidae). | genomes of opisthorchid species are characterized by small size, suggesting a reduced amount of repetitive dna in their genomes. distribution of repetitive dna sequences in the chromosomes of five species of the family opisthorchiidae (opisthorchis felineus 2n=14 (rivolta, 1884), opisthorchis viverrini 2n=12 (poirier, 1886), metorchis xanthosomus 2n=14 (creplin, 1846), metorchis bilis 2n=14 (braun, 1890), clonorchis sinensis 2n=14 (cobbold, 1875)) was studied with c- and agnor-banding, generatio ... | 2011 | 21791251 |
| light and electron microscopy observations of embryogenesis and egg development in the human liver fluke, opisthorchis viverrini (platyhelminthes, digenea). | eggs of most species digenean flukes hatch in the external environment to liberate larvae that seek and penetrate a snail intermediate host. those of the human liver flukes, opisthorchis viverrini, hatch within the gastrointestinal canal of their snail hosts. while adult parasites are primarily responsible for the pathology in cases of human opisthorchiasis, their eggs also contribute by inducing granulomata and in serving as nidi for gallstone formation. in view of the peculiar biology of o. vi ... | 2011 | 21786067 |
| a novel carbohydrate antigen expression during development of opisthorchis viverrini- associated cholangiocarcinoma in golden hamster: a potential marker for early diagnosis. | poor prognosis of cholangiocarcinoma (cca) is primarily due to delayed diagnosis because of the lack of appropriate tumor marker(s) to detect cancer development at an early stage. we have recently established a s121 monoclonal antibody (mab) which recognizes an unidentified glycan epitope on muc5ac, designated as cca-associated carbohydrate antigen (cca-ca). this antigen is expressed in human cca cells but not in normal biliary epithelia. detection of cca-ca effectively distinguished cca patient ... | 2011 | 21784171 |
| cloning, expression, and characterization of a novel opisthorchis viverrini calcium-binding ef-hand protein. | a novel 22.8kda of opisthorchis viverrini (ov) calcium-binding ef-hand protein (ov cabp) was identified and isolated from an immunoscreening of the adult stage ov cdna library by using a human cholangiocarcinoma (cca) serum. this protein was related to other calcium-binding proteins and conserved among the trematodes. ov cabp shared 98% amino acid identity to 22.8kda of clonorchis sinensis cabp and both were classified as a new group of cabp ef-hand protein by multiple sequence alignment and phy ... | 2011 | 21782972 |
| secreted opisthorchis viverrini glutathione s-transferase regulates cell proliferation through akt and erk pathways in cholangiocarcinoma. | opisthorchis viverrini can develop mitogenic substances into the excretory/secretory product (esp) that may play an important role in promoting the genesis of cholangiocarcinoma (cca). in the present study, glutathione s-transferase (gst) is identified as being secreted into ov-esp and acting as one of the parasitic mitogens. its proliferative effect and possible mechanism were explored and its association with the tumor development is proposed. ov-esp was concentrated and purified by gel filtra ... | 2011 | 21782971 |
| overexpression of pdgfa and its receptor during carcinogenesis of opisthorchis viverrini-associated cholangiocarcinoma. | cholangiocarcinoma (cca) is a crucial health problem in northeastern part of thailand, which is caused by a combination of opisthorchis viverrini infection and nitrosamine. a better understanding of its molecular mechanism is an important step to discover and develop the new diagnostics and therapies for cca. to reveal the involvement of potential genes in the development of cca, the present study investigated the expression kinetics of platelet-derived growth factor alpha (pdgfa) and its recept ... | 2011 | 21777692 |
| ultrasonography assessment of hepatobiliary abnormalities in 3359 subjects with opisthorchis viverrini infection in endemic areas of thailand. | a cross sectional study on hepatobiliary abnormalities in opisthorchiasis was performed in 8936 males and females aged from 20 to 60years from 90 villages of khon kaen province, northeast thailand. all were stool-examined for opisthorchis viverrini infection by standard quantitative formalin/ethyl acetate concentration technique. of these, 3359 participants with stool egg positive underwent ultrasonography of the upper abdomen. the hepatobiliary abnormalities detected by ultrasound are described ... | 2011 | 21771664 |
| opisthorchis viverrini-antigen induces expression of marcks during inflammation-associated cholangiocarcinogenesis. | myristoylated alanine rich c kinase substrate (marcks) has been implicated in pkc-mediated membrane-cytoskeleton alterations that underlie lipopolysaccharide (lps)-induced macrophage responses. marcks is postulated to be involved in inflammation-associated cca based on its overexpression in cholangiocarcinoma (cca) and inflammatory cells. the aims of this study were to investigate localization patterns of marcks in hamster and human tissue during cholangiocarcinogenesis and to examine the involv ... | 2011 | 21763456 |
| expression of oxysterol binding protein isoforms in opisthorchiasis-associated cholangiocarcinoma: a potential molecular marker for tumor metastasis. | oxysterols are oxygenated derivatives of cholesterol generated by enzymatic reactions mediated by cytochrome p450 family enzymes or by inflammation-associated non-enzymatic reactions. oxysterol binding proteins (osbps) are cytosolic high affinity receptors for oxysterols. we previously found that osbpl-8 is upregulated in liver fluke (opisthorchis viverrini)-induced hamster cholangiocarcinoma (cca). our aims were to determine the expression patterns of osbp isoforms in human cca tissues and to e ... | 2011 | 21763455 |
| adult opisthorchis viverrini flukes in humans, takeo, cambodia. | to the editor: opisthorchis viverrini and clonorchis sinensis, the 2 major species of small liver flukes (family opisthorchiidae), cause chronic inflammation in the bile duct, which leads to cholangitis and cirrhosis of the liver, and are a predisposing factor for cholangiocarcinoma (1). human infections with o. viverrini flukes are found along riverside areas of indochina (thailand, lao people's democratic republic [pdr], and vietnam) (2). | 2011 | 21762595 |
| genome size estimation of liver fluke opisthorchis viverrini by real-time polymerase chain reaction based method. | the human liver fluke, opisthorchis viverrini, has been categorized as a class one carcinogenic organism according to its strong association with cholangiocarcinoma, bile duct cancer which has high incidence in the northeast of thailand. the lack of genome database of this parasite limited the studies aimed to understand the basic molecular biology of this carcinogenic liver fluke. the determination of the genome size is an initial step prior to the full genome sequencing. in this study, we appl ... | 2011 | 21757028 |
| expression, refolding and purification of ov-grn-1, a granulin-like growth factor from the carcinogenic liver fluke, that causes proliferation of mammalian host cells. | granulins (grns) are potent growth factors that are upregulated in many aggressive cancers from a wide range of organs. grns form tight, disulphide bonded, beta hairpin stacks, making them difficult to express in recombinant form. we recently described ov-grn-1, a grn family member secreted by the carcinogenic liver fluke of humans, opisthorchis viverrini, and showed that recombinant ov-grn-1 expressed and refolded from escherichia coli caused proliferation of mammalian cell lines at nanomolar c ... | 2011 | 21757010 |
| hepatic cytochrome p450 2a6 and 2e1 status in peri-tumor tissues of patients with opisthorchis viverrini-associated cholangiocarcinoma. | endogenous nitrosation due to chronic inflammation is enhanced in opisthorchiasis and plays a crucial role in the development of cholangiocarcinoma (cca). hepatic cytochrome p450 (cyp) family enzymes, especially cyp2a6 and cyp2e1, are involved in the metabolism of procarcinogens; these two enzymes metabolize endogenous nitrosamines to carcinogenic n-dimethylnitrosamine (ndma). cyp2a6 activity is increased in patients infected with opisthorchis viverrini. our aim was to determine whether the expr ... | 2011 | 21745589 |
| high prevalence of opisthorchis viverrini infection in reservoir hosts in four districts of khon kaen province, an opisthorchiasis endemic area of thailand. | khon kaen, a northeastern province of thailand, has been considered as one of the human opisthorchiasis endemic areas with continuing high prevalence. unsuccessful eradication of the disease is probably from the culture of eating raw and undercooked fish of local residence and the parasitic persistency in animal reservoir hosts, such as cats and dogs. in cooperation with the other human opisthorchiasis control programs in an endemic area of 29 villages in ban haet, ban phai, chonnabot and muncha ... | 2011 | 21843654 |
| evaluation of liver fluke recombinant cathepsin b-1 protease as a serodiagnostic antigen for human opisthorchiasis. | a cathepsin b-like cysteine protease belonging to family c1 is abundantly expressed in the transcriptome and proteome of the carcinogenic liver fluke of humans, opisthorchis viverrini. this enzyme is present in excretory/secretory (es) products released by parasites cultured in vitro. this study evaluated the performance of recombinant o. viverrini cathepsin b1 (rov-cb-1) as an antigen for immunodiagnosis of opisthorchiasis. the full length ov-cb-1 cdna was cloned and recombinant protein was pro ... | 2011 | 21704728 |
| molecular expression and enzymatic characterization of thioredoxin from the carcinogenic human liver fluke opisthorchis viverrini. | the human liver fluke, opisthorchis viverrini, induces inflammation of the hepatobiliary system. despite being constantly exposed to inimical oxygen radicals released from inflammatory cells, the parasite survives for years. defense against oxidative damage can be mediated through glutathione and/or thioredoxin utilizing systems. here, we report the molecular expression and biochemical characterization of a thioredoxin (trx) from o. viverrini. o. viverrini trx cdna encoded a polypeptide of 105 a ... | 2011 | 21740981 |
| the liver fluke opisthorchis viverrini expresses nitric oxide synthase but not gelatinases. | host-parasite interaction during infection with the liver fluke opisthorchis viverrini plays an important role in opisthorchiasis-associated cholangiocarcinoma via nitric oxide (no) production. host cells induce nitric oxide synthase (nos)-dependent dna damage and secrete ras-related c3 botulinum toxin substrate (rac)1, heme oxygenase (ho)-1, and gelatinases (matrix metalloproteinase (mmp)2 and mmp9). we evaluated whether these enzymes are expressed in o. viverrini. colocalization of nos and rac ... | 2011 | 21718800 |
| the systematics and population genetics of opisthorchis viverrini sensu lato: implications in parasite epidemiology and bile duct cancer. | together with host and environmental factors, the systematics and population genetic variation of opisthorchis viverrini may contribute to recorded local and regional differences in epidemiology and host morbidity in opisthorchiasis and cholangiocarcinoma (cca). in this review, we address recent findings that o. viverrini comprises a species complex with varying degrees of population genetic variation which are associated with specific river wetland systems within thailand as well as the lao pdr ... | 2011 | 21821147 |
| telomeric dna in chromosomes of five opisthorchid species. | the analysis of telomere repeat distribution in chromosomes of five opisthorchid species (opisthorchis felineus (rivolta, 1884), opisthorchis viverrini (poirier, 1886), metorchis xanthosomus (creplin, 1846), metorchis bilis (braun, 1890), clonorchis sinensis (cobbold, 1875)) was performed with fluorescent in situ hybridization (fish) of labeled (ttaggg)n dna-probe and pna telomere probe on mitotic and meiotic chromosomes of these species. it was shown that chromosome telomeres of all studied spe ... | 2011 | 21708281 |
| inflammatory response to liver fluke opisthorchis viverrini in mice depends on host master coregulator mta1, a marker for parasite-induced cholangiocarcinoma in humans. | based on the recently established role for the master coregulator mta1 and mta1-containing nuclear remodeling complexes in oncogenesis and inflammation, we explored the links between parasitism by the carcinogenic liver fluke opisthorchis viverrini and this coregulator using both an mta1(-/-) mouse model of infection and a tissue microarray of liver fluke-induced human cholangiocarcinomas (ccas). intense foci of inflammation and periductal fibrosis in the liver and kidneys of wild-type mta1(+/+) ... | 2011 | 21725997 |
| families opisthorchiidae and heterophyidae: are they distinct? | superfamily opisthorchioidea looss, 1899 consists of three well-known families, opisthorchiidae, heterophyidae, and cryptogonimidae, with basic similarities in morphology and life-cycles. many species in the first two of these families are human pathogens, such as opisthorchis viverrini, o. felineus, clonorchis sinensis, haplorchis spp. and metagonimus spp. recently, a molecular phylogenetic study on the classification of digenea revealed a paraphyletic relationship between opisthorchiidae and h ... | 2011 | 21740979 |
| oxidative and nitrative dna damage: key events in opisthorchiasis-induced carcinogenesis. | chronic inflammation induced by liver fluke (opisthorchis viverrini) infection is the major risk factor for cholangiocarcinoma (cca) in northeastern thailand. increased levels of proinflammatory cytokines and nuclear factor kappa b that control cyclooxygenase-2 and inducible nitric oxide activities, disturb the homeostasis of oxidants/anti-oxidants and dna repair enzymes, all of which appear to be involved in o. viverrini-associated inflammatory processes and cca. consequently oxidative and nitr ... | 2011 | 21704729 |
| specific serum igg, but not iga, antibody against purified opisthorchis viverrini antigen associated with hepatobiliary disease and cholangiocarcinoma. | opisthorchiasis caused by opisthorchis viverrini infection induces hepatobiliary disease (hbd)-associated cholangiocarcinoma (cca) via a chronic inflammatory immune response. here, we evaluated specific igg and iga antibodies against different fractions of o. viverrini antigen in residents from an endemic community in northeast thailand with varying hepatobiliary abnormalities. crude somatic o. viverrini antigen was purified into three fractions (viz., p1, p2 and p3) by gel infiltration chromato ... | 2011 | 21718798 |
| the ecology of the bithynia first intermediate hosts of opisthorchis viverrini. | opisthorchiasis, together with its associated cholangiocarcinoma, is one of the most important human parasitic diseases on continental southeast asia. a great deal of epidemiological data from humans is available on this disease, particularly from the northeast of thailand, however, only limited information is available on those aspects of the life cycle relating to its bithynia (gastropoda) and cyprinid fish intermediate hosts. here we review the information which is available on the bithynia h ... | 2011 | 21821148 |
| discovery of opisthorchis lobatus (trematoda: opisthorchiidae): new recorded species of small liver flukes in the greater mekong sub-region. | abstract metacercariae, morphologically similar to those of small liver flukes, were found to parasitize red-tailed snakehead fish, channa limbata, collected from the city of vientiane, lao pdr. adult worms that were recovered from experimentally-infected hamsters showed characteristics distinctly different from opisthorchis viverrini but closely similar to opisthorchis lobatus, which was first reported in poultry (anas sp.) from pakistan. the present study aimed to redescribe o. lobatus based o ... | 2011 | 21682557 |
| echinostoma ilocanum infection in oddar meanchey province, cambodia. | fecal examinations using the kato katz technique were performed on a total of 1,287 villagers (945 students and 342 general inhabitants) of oddar meanchey province, cambodia in may 2007 and november 2009. the overall intestinal helminth egg positive rate was 23.9%, and the most prevalent helminth species was hookworms (21.6%). other helminth eggs detected included echinostomes (1.0%), enterobius vermicularis (0.8%), small trematode eggs (0.7%), which may include opisthorchis viverrini and haplor ... | 2011 | 21738278 |
| development of a taqman based real-time pcr assay for detection of clonorchis sinensis dna in human stool samples and fishes. | clonorchiasis caused by the oriental liver fluke clonorchis sinensis is a fish-borne zoonosis endemic in a number of countries. this article describes the development of a taqman based real-time pcr assay for detection of c. sinensis dna in human feces and in fishes. primers targeting the first internal transcribed spacer (its-1) sequence of the fluke were highly specific for c. sinensis, as evidenced by the negative amplification of closely related trematodes in the test with the exception of o ... | 2011 | 21729765 |
| Raw attitudes, wetland cultures, life-cycles: Socio-cultural dynamics relating to Opisthorchis viverrini in the Mekong Basin. | Opisthorchis viverrini is one of the most common and medically important food-borne parasites in the Lower Mekong area of Southeast Asia. As we learn more about its ecology, pathology and epidemiology we see the need to consider more deeply the socio-cultural dynamics with which food-borne species complexes are associated. This paper argues that the Mekong region is characterized by strong livelihoods and life-style associations within wetland ecosystems, which are inseparable from human eating ... | 2011 | 21712097 |
| Diagnostic values of parasite-specific antibody detections in saliva and urine in comparison with serum in opisthorchiasis. | Infection by the liver fluke (Opisthorchis viverrini) causes hepatobiliary disease and bile duct cancer (cholangiocarcinoma, CCA) in endemic areas in Southeast Asia. Measurements of humoral immune response particularly parasite-specific antibodies are useful not only for serodiagnosis but they have been implicated as risk factors of CCA. In this study, we used indirect Enzyme Immunosorbent Assay (ELISA) to measure O. viverrini-specific immunoglobulins in serum, urine and saliva and assessed effi ... | 2011 | 21704727 |
| co-infection with opisthorchis viverrini and haplorchis taichui detected by human fecal examination in chomtong district, chiang mai province, thailand. | diseases caused by the liver fluke, opisthorchis viverrini and the minute intestinal fluke, haplorchis taichui, are clinically important, especially in the northeast and north regions of thailand. it is often difficult to distinguish between these trematode species using morphological methods due to the similarity of their eggs and larval stages both in mixed and co-infections. a sensitive, accurate, and specific detection method of these flukes is required for an effective epidemiological contr ... | 2012 | 22047704 |
| Situation Analysis of Risk Factors Related to Non-communicable Diseases in Khon Kaen Province, Thailand. | A descriptive cross-sectional study was carried out in Khon Kaen Province during January 1 to June 30, 2008. The aims were to assess: (1) the prevalence of risk factors for chronic diseases such as cancer, diabetes, blood pressure; and (2) health behaviour and health education needs. There were 338 sample subjects aged between 20-60 years, from urban, semi-urban and rural areas. Some 20.4 % of the sample subjects reported that they were unhealthy (10.4% diagnosed with hypertension, 9.8% with dia ... | 2011 | 21875293 |
| Liver fluke-induced hepatic oxysterols stimulate DNA damage and apoptosis in cultured human cholangiocytes. | Oxysterols are cholesterol oxidation products that are generated by enzymatic reactions through cytochrome P450 family enzymes or by non-enzymatic reactions involving reactive oxygen and nitrogen species. Oxysterols have been identified in bile in the setting of chronic inflammation, suggesting that biliary epithelial cells are chronically exposed to these compounds in certain clinical settings. We hypothesized that biliary oxysterols resulting from liver fluke infection participate in cholangio ... | 2011 | 22044627 |
| Anti-inflammatory effect of prednisolone on the growth of human liver fluke in experimental opisthorchiasis. | Opisthorchis viverrini is one of the risk factors for cholangiocarcinoma (CCA) development and is endemic in Southeast Asia including Thailand. CCA is induced by chronic inflammation from a combination of mechanical damage, parasite secretions, and immunopathology. Chronic infection with O. viverrini has been associated with several hepatobiliary diseases which affect the development of hepatobiliary cancer and CCA. Therefore, reducing the pathogenesis from O. viverrini infection may be one of t ... | 2011 | 22186974 |
| Increased expression of TLR-2, COX-2, and SOD-2 genes in the peripheral blood leukocytes of opisthorchiasis patients induced by Opisthorchis viverrini antigen. | Re-infection with liver fluke, Opisthorchis viverrini, increases proinflammatory molecules involved in inflammation-mediated disease and carcinogenesis in an animal model. To clarify whether these genes respond to parasite antigen in peripheral blood leukocytes (PBL) of opisthorchiasis patients, we examined the transcriptional level of oxidant-generating (toll-like receptor 2 (TLR-2), nuclear factor-kappa B (NF-KB), and cyclooxygenase 2 (COX-2)), anti-oxidant-generating (manganese superoxide dis ... | 2011 | 22160279 |