| eosinophil chemotactic lymphokine produced by spleen cells of schistosoma japonicum-infected mice. | eosinophil chemotactic factor (ecf) was detected in the cell-free supernatant of the culture of spleen cells obtained from schistosoma japonicum-infected mice. ecf production by spleen cells was dependent on both the amount of soluble egg antigen (sea) added and the number of cells in the culture. when sufficient amount of sea was added to the culture, ecf production was detectable by 6 h after incubation, reached a plateau by 24 h and then rapidly decreased. ecf was produced by nonadherent, thy ... | 1987 | 3100450 |
| the induction of species-specific immunity against schistosoma japonicum by exposure of rats to ultra-violet attenuated cercariae. | single percutaneous immunizations of fischer rats with 1000 ultra-violet attenuated schistosoma japonicum cercariae induced 52-88% resistance to challenge 4 weeks later. increasing this to 3 immunizations induced 90% resistance to challenge, and this level of protection remained undiminished for up to 40 weeks after vaccination. rats vaccinated with gamma-irradiated s. mansoni cercariae were resistant to challenge with s. mansoni but not s. japonicum. similarly rats vaccinated with u.v.-attenuat ... | 1987 | 3103047 |
| modulation of schistosoma japonicum pulmonary egg granulomas with monoclonal antibodies. | we have examined the ability of various schistosoma japonicum egg antigens to sensitize mice for subsequent secondary pulmonary egg granuloma formation. further, we produced monoclonal antibodies (mabs) specific for egg antigens and evaluated their abilities to modulate pulmonary granuloma formation and inhibit soluble egg antigen (sea)-stimulated lymphocyte blastogenesis. a homogeneous, 140 kd egg glycoprotein, sje; 140-gp was nearly as effective as intact eggs in sensitizing for egg-focused pu ... | 1987 | 3103476 |
| [a comparative study of cop and iha in the epidemiological evaluation of schistosomiasis japonica in eradicated endemic area]. | | 1986 | 3105910 |
| [effects of praziquantel and artemether on tegumental surface antigens of different developmental stages of schistosoma japonicum]. | | 1986 | 3105912 |
| comparative efficacy of praziquantel and its optic isomers in experimental therapy of schistosomiasis japonica in rabbits. | | 1986 | 3105969 |
| the involvement of eggs and soluble egg antigens in resistance to schistosoma japonicum. | mice presensitized with sea and subsequently injected with s. japonicum eggs into their lungs or liver developed pathology similar to that found in infected mice and were consequently resistant to challenge (average 33% and 53%, respectively). however, soluble egg antigens were also capable of inducing low levels of resistance in mice (average 22.5%), but intact eggs alone injected into the lungs, liver or subcutaneous tissues were not. thus, prior sensitization to 'marginally' protective solubl ... | 1987 | 3106466 |
| [relationship between the efficacy of praziquantel on rabbits infected with schistosoma japonicum and the immune level of the host]. | | 1986 | 3107342 |
| ileocolonic schistosomiasis presenting as lymphoma. | the blood flukes of the genus schistosome have a wide geographic distribution. schistosoma japonicum is found in the far east while schistosoma mansoni is found in africa, south america, the middle east, and the carribean. there are numerous other species of schistosome; however, japonicum, mansoni, and hematobium account for the majority of human disease. infection of the gastrointestinal tract is not uncommon in the united states. recognition of this disease is important for appropriate medica ... | 1987 | 3107371 |
| reactivity of two major allergens isolated from schistosoma japonicum eggs against ige and igg antibodies in human serum. | antigenicity and specificity of two major allergens termed j1 and j2, both of which had been purified from soluble egg antigen (sea) preparation of schistosoma japonicum, were examined against serum from schistosomiasis japonica patients. by using enzyme-linked immunosorbent assay, specific ige or igg levels to j1 or j2 in the patients' sera correlated well with those to crude sea, indicating that j1 and j2 are the major antigens in sea binding human ige and igg antibodies. binding inhibition st ... | 1987 | 3108166 |
| hla-dq is epistatic to hla-dr in controlling the immune response to schistosomal antigen in humans. | antigens that produce an antibody response in some members of a species may fail to do so in others. the response to an antigen is controlled by a gene termed the immune response (ir) gene, which is transmitted as a single dominant trait. we have provided evidence for similar immune suppression (is) genes which control non-responsiveness through the antigen specific suppressor t cell. the non-responsiveness is also dominantly inherited and the is genes are linked to the histocompatibility (hla) ... | 1987 | 3108669 |
| eosinophil chemotactic lymphokine produced by spleen cells of schistosoma japonicum-infected mice. ii. physicochemical heterogeneity of eosinophil chemotactic lymphokines selective to bone marrow- or peritoneal exudate-eosinophils. | the physicochemical nature of eosinophil chemotactic factor (ecf) was examined by using different maturation stages of eosinophils, namely eosinophils obtained from bone marrow (bm-eo) and those obtained from peritoneal cavity (pec-eo), as the indicator of in vitro chemotaxis. as the ecf source, the conditioned medium of the culture of spleen cells obtained from schistosoma japonicum-infected mice was used. when the conditioned medium was chromatographed by sephadex g75, apparent molecular weigh ... | 1987 | 3110075 |
| collaborative study on antigens for immunodiagnosis of schistosoma japonicum infection. | six research laboratories in australia, japan, the philippines and the usa participated in a collaborative evaluation of immunodiagnostic tests for schistosoma japonicum infections. the serum bank consisted of 385 well-documented sera from brazil, kenya, philippines, republic of korea and europe. twelve s. japonicum antigen/test system combinations were evaluated.crude s. japonicum egg antigens showed the highest sensitivity and specificity. the defined or characterized antigens showed no advant ... | 1987 | 3111737 |
| passive transfer of resistance to mice with sera from rabbits, rats or mice vaccinated with ultraviolet-attenuated cercariae of schistosoma japonicum. | all serum transfers from donor rats or rabbits given single or multiple vaccinations of ultraviolet (u.v.)-attenuated schistosoma japonicum cercariae conferred significant resistance against challenge to mice. donors given 5 vaccinations, however, produced the most effective sera; rat sera giving up to 88% protection and rabbit sera up to 80%. this protective effect was species-specific and titratable. sera from vaccinated rabbits and rats were were most effective when transferred to mice 2 h be ... | 1987 | 3112703 |
| loss of resistance to reinfection with schistosoma japonicum in mice after treatment with praziquantel. | seven-week-old adult schistosoma japonicum worms were eliminated from mice within one week of treatment with praziquantel and oviposition ceased within 2 days. the excretion of faecal eggs ceased within 2 weeks of treatment. the numbers of eggs retained in the intestine and those in the liver remained constant for up to 20 weeks after cure. untreated, infected mice were on average 80% resistant to reinfection and mice challenged one week after treatment were equally resistant. however, reduction ... | 1987 | 3113003 |
| immunogenetic factors involved in the pathogenesis of distinct clinical manifestations of schistosomiasis japonica in the philippine population. | immunogenetic factors were studied in 60 patients with schistosomiasis japonica in the philippines, of whom 15 were characterized by marked hepatosplenic lesions and 45 characterized by cerebral symptoms. immune responsiveness of the patients to schistosomal antigen was measured by t cell proliferation in vitro, and their hla-a and -b specificities were typed. all but one hepatosplenic patients showed strong immune responsiveness to the schistosomal antigen, whereas both low and high responders ... | 1987 | 3113004 |
| prevalence of renal involvement in schistosoma japonicum infection. | 244 outpatients and 100 hospitalized patients with confirmed schistosoma japonicum infection were prospectively surveyed for the presence of nephropathy. there was no association between schistosomiasis and renal disease in the outpatient group. three hospitalized patients had evidence of significant nephropathy, but this number was not significantly higher than in a control group of 100 hospitalized age and sex-matched control patients without schistosomiasis. one schistosomiasis patient with s ... | 1987 | 3113006 |
| granulocyte-macrophage colony-stimulating factor enhances the production of eosinophil chemotactic lymphokine by egg-associated granulomas of schistosoma japonicum-infected mice. | granulocyte-macrophage colony-stimulating factor (gm-csf) produced by splenic lymphocytes obtained from schistosoma japonicum-infected mice was partially purified by a combination of deae anion-exchange chromatography, concanavalin a-sepharose affinity chromatography, and high-pressure liquid chromatography. when this partially purified gm-csf was added to the culture of isolated intact granulomas, eosinophil chemotactic factor (ecf) lymphokine production by granulomas was significantly enhanced ... | 1987 | 3114144 |
| production and properties of a mouse monoclonal ige antibody to schistosoma japonicum. | a monoclonal ige antibody was prepared by fusion of ns-1 myeloma cells with spleen cells of c3h/he mice immunized with an extract of adult worms of schistosoma japonicum (sj). the antibody was able to elicit passive cutaneous anaphylaxis in the rat skin against sj with the highest titer of 1/256,000 in an ascitic form but did not cross-react with any of antigens extracted from s. mansoni, fasciola hepatica, paragoniumus westermani, or trichinella spiralis. western blot analysis indicated that th ... | 1987 | 3114384 |
| [effects of schistosome-derived metabolic products on the proliferative response in mice infected with schistosoma japonicum]. | | 1987 | 3115622 |
| sera of schistosoma japonicum-infected patients cross-react with diagnostic 31/32 kd proteins of s. mansoni. | schistosoma mansoni adult worm antigens were tested for cross-reactions with sera obtained from patients infected with s. japonicum. the sera consistently recognized a doublet of bands, in immunoblots, which had molecular weights of approximately 31 and 32 kilodaltons (kd). this reaction was found to be markedly reduced with sera of patients who had received chemotherapy and who had a low risk of reinfection. sera obtained from uninfected persons or from patients infected with other parasites ne ... | 1987 | 3115655 |
| schistosoma japonicum: immunocytochemistry of adults using heterologous antiserum to bovine cathepsin d. | results from previous biochemical and cytochemical studies show that a pepstatin-sensitive hemoglobinase is present in the gastrodermis of adult schistosoma japonicum. to determine whether there is structural similarity to mammalian cathepsin d in addition to inhibition similarity, an immunocytochemical study was initiated using heterologous antiserum to bovine cathepsin d. with light microscopy, immunostaining was observed in the cecal lumen, the gastrodermis, and on the dorsal tegument and tub ... | 1987 | 3115814 |
| eosinophil chemotactic lymphokine produced by spleen cells of schistosoma japonicum-infected mice. iii. isolation and characterization of two distinctive eosinophil chemotactic lymphokines directed against different maturation stages of eosinophils. | two distinctive types of eosinophil chemotactic lymphokines (ecf-l) directed against eosinophils obtained from bone marrow (bm-eo) or from peritoneal cavity (pec-eo) were isolated from the media conditioned by spleen cells from mice infected with schistosoma japonicum by a combination of anion-exchange chromatography on de52, affinity chromatography on procion red agarose, and high-performance liquid chromatography. the molecular weight of ecf-l directed against bm-eo was 10,000 and that against ... | 1987 | 3115903 |
| studies on the immunological disturbance in murine schistosomiasis japonica from the viewpoint of the interleukin cascade reaction. | we examined the mechanisms of disturbed t-lymphocyte function that occurred during schistosoma japonicum infection of balb/c mice from the viewpoint of the interleukin cascade reaction. each point of the interleukin cascade reaction was examined. first, il-1 production by adherent peritoneal or spleen cells, as a source of macrophages, was normal or rather enhanced during the infection, the values being 100-180% of the control. secondly, proliferative response to exogenous il-1 of thymocytes fro ... | 1987 | 3119470 |
| schistosomiasis in malaysia. | schistosomiasis was discovered in malaysia in 1975 in an autopsy case. since 1975 autopsies, surveys, and resurveys have been carried out to identify animal hosts, snail intermediate hosts, and reservoir hosts. seroepidemiologic tests involving enzyme-linked immunosorbent and circumoval precipitin methods have been used to determine the true incidence and prevalence of this protean disease among the orang aslis (aborigines) in malaysia. with the use of better epidemiologic and parasitologic tool ... | 1987 | 3120271 |
| secondary amyloidosis in normal and immunocompromised mice infected with schistosoma japonicum. | secondary amyloidosis developed in various tissues of immunologically intact and t-cell deprived cba mice infected with schistosoma japonicum as indicated by histological, histochemical and immunofluorescence techniques. mice deprived of t-cells were most susceptible both in terms of the timing of the onset of amyloidosis, first noted 6 weeks post-infection, and in terms of the incidence, reaching 100% 12 weeks post-infection. amyloidosis developed later in the infection in immunologically intac ... | 1987 | 3122814 |
| [comparative efficacy of levopraziquantel and praziquantel in the treatment of schistosoma japonicum infection]. | | 1987 | 3123170 |
| recovery from immunesuppression in mice infected with schistosoma japonicum by treatment of praziquantel. | to learn the possible alteration of immune response, hemolytic plaque-forming cells (pfc) produced in the spleen of schistosoma japonicum infected mice treated with praziquantel and untreated group were counted. there was no significant difference in the immunesuppression percentage between the treated and untreated groups 1 and 3 weeks after treatment. in 5 weeks after treatment, however, the immunesuppression percentage in the treated mice was markedly reduced in comparison with that of the un ... | 1987 | 3123753 |
| epidemiologic studies on schistosomiasis japonica in a newly found habitat of oncomelania snails in japan. | epidemiologic studies' on schistosomiasis japonica were carried out in the obitsu river basin of central boso peninsula, a newly found habitat of oncomelania snails in japan. forty-five (4.1%) of 1,102 schoolchildren and 64 (10.4%) of 618 junior high school students were positive for the skin test with veronal buffered saline-extracted schistosoma japonicum antigen. none was positive by the enzyme-linked immunosorbent assay, complement fixation test, double diffusion test in agar, and circumoval ... | 1988 | 3124649 |
| praziquantel pharmacokinetics and side effects in schistosoma japonicum-infected patients with liver disease. | praziquantel undergoes extensive first-pass hepatic biotransformation, but there is little information on its disposition or toxicity when administered to patients with liver disease. to define the influence of liver disease on the pharmacokinetics of praziquantel, we administered it orally to 30 patients with proven schistosoma japonicum infection whose liver disease was carefully assessed as being severe, moderate, or absent. both the peak plasma concentration of praziquantel and the bioavaila ... | 1988 | 3125260 |
| schistosomal glomerulopathy: selection factors. | of several hundreds of millions of people infested with schistosomiasis, only a few hundreds have, so far, been documented to have one or other of the three schistosoma-associated immune-mediated glomerulopathies, namely proliferative glomerulonephritis, focal and segmental sclerosis, and amyloidosis. regardless of undoubted under-reporting, some factors must be involved in the selection of those who develop such glomerulopathies. on the basis of experimental and clinical evidence, this review h ... | 1987 | 3126449 |
| susceptibility of schistosoma japonicum to different developmental stages to praziquantel. | | 1987 | 3127152 |
| in vitro analysis of t-cell-mediated immunity to intact eggs in murine experimental schistosoma japonicum infection. | to characterize the mechanisms of induction and regulation of the cell population involved in granuloma formation around eggs of schistosoma japonicum, we utilized a simple method of in vitro experiments. lyt1+2-t cells were essential for in vitro responses to the intact s. japonicum eggs, which were assumed to be comparable to in vivo granulomatous responses. t-cell responses seemed to be macrophage-dependent, and responding t cells produced il-2-like activities in the culture supernatant. sera ... | 1987 | 3127618 |
| [efficacy of praziquantel silicone elastomers on animals infected with schistosoma japonicum]. | | 1987 | 3128057 |
| lack of effect of unmated schistosomes on the fecundity of mated worm pairs. | an imbalance between the numbers of male and female worms had no significant effect upon the fecundity of schistosoma mansoni in rhesus monkeys or upon the number of s. mansoni eggs in the tissues of infected persons or mice. the number of s. japonicum eggs in the tissues of infected rabbits was similarly unaffected by disproportion between worm genders. | 1988 | 3128653 |
| [susceptibility of the chinese hamster (cricetulus griseus) to parasitic infection. (2) experimental infection with schistosoma japonicum]. | chinese hamsters (cricetulus griseus milne-edwards: cha colony) were experimentally infected with schistosoma japonicum cercariae by the percutaneous route and intraperitoneal inoculation to confirm their susceptibility to the infection with this parasite in comparison with that of mice (dd). chinese hamsters proved to be highly susceptible to percutaneous and intraperitoneal infection with s. japonicum. the eggs of s. japonicum were recognized in feces of all chinese hamsters and mice from abou ... | 1988 | 3129351 |
| evidence of anti-embryonation immunity and egg destruction in mice sensitized with immature eggs of schistosoma japonicum. | balb/c mice sensitized by repeated injections of immature eggs of the trematode worm, schistosoma japonicum, were challenged with low numbers of cercariae and evidence was sought for inhibition of embryonation by examination of eggs in livers and intestines at days 40 - 42 of infection. in contrast to the situation in unsensitized control mice, a greater proportion of dead eggs was noted in tissues of many of egg-sensitized mice. there was also a decrease in the proportion of mature eggs relativ ... | 1987 | 3130066 |
| [competitive elisa using monoclonal antibody for the diagnosis of schistosomiasis japonica]. | | 1987 | 3130156 |
| hepatoma formation in ddy mice with chronic schistosomiasis japonica. | three hundred and ninety-five four-week-old spf female ddy mice were each exposed to 5 or 6 cercariae of schistosoma japonicum (japanese strain) on their shaved abdomens and were maintained in a conditioned clean environment and fed on sterilized food and water. fecal examinations at 8 to 10 weeks postinfection (pi) revealed 169 mice to be infected. more than half of them died within 30 weeks pi and 70 mice that survived to the 50th week pi were sacrificed. at autopsy, we could find no schistoso ... | 1988 | 3130351 |
| [histochemical studies of artemether, fuvinazole and niridazole on schistosomula of schistosoma japonicum and mouse livers]. | | 1987 | 3130747 |
| [detection of enzyme dissociated scic from sera of schistosomiasis japonica by two-direction counter-immunoelectrophoresis]. | | 1987 | 3131037 |
| [observation on the stability of schistosome egg output in final host feces]. | | 1987 | 3131039 |
| negative conversion of cop (circumoval precipitation) reactions after selective mass chemotherapy on schistosomiasis japonica with praziquantel in bohol, philippines. | for the longitudinal survey of human schistosomiasis japonica in the villages of sto. tomas and san vicente in the province of bohol, philippines, the circumoval precipitin (cop) test was used for case detection. plasma collected by finger prick from the community people and lyophilized eggs of schistosoma japonicum were used for the assay. degree of precipitation at type 2 or 3 of yokogawa et al. [1] was considered as positive. selective mass chemotherapy with praziquantel was given to the pati ... | 1987 | 3131561 |
| [the application of the indirect fluorescent antibody test (ifat) in epidemiological surveys on schistosomiasis japonica]. | | 1988 | 3133118 |
| [evaluation of urea-soluble egg antigen (jeu) in the diagnosis and assessment of cure of schistosomiasis japonica]. | | 1988 | 3133133 |
| ultrastructure of skin-phase schistosomula of schistosoma japonicum by transmission electron microscopy. | | 1987 | 3133177 |
| [preliminary report on the diagnosis of schistosomiasis japonica by the indirect fluorescent antibody test and immunoenzyme staining technic]. | | 1988 | 3133180 |
| immunoblotting analysis of the major integral membrane protein antigens of schistosoma japonicum. | triton x-114 has been employed to isolate integral membrane proteins from schistosoma japonicum and schistosoma mansoni adult worms. suitable marker molecules and antisera directed or raised against schistosome proteins partitioned by triton x-114 extraction indicated that the phase separation and purification of integral membrane proteins had been successful and this fraction was free of contamination with aqueous (soluble) or secretory antigens. two dimensional immunoblots further exemplified ... | 1988 | 3133562 |
| schistosomiasis japonica: epidemiology and genetic aspects. | | 1988 | 3136552 |
| worm burden and lymphocyte response in mice immunized with n-methyl-n'-nitro-n-nitrosoguanidine attenuated cercariae of schistosoma japonicum. | | 1988 | 3136992 |
| immunologic diagnosis of schistosomiasis japonica by indirect immunoperoxidase on liver-egg section (le-iip). | | 1988 | 3136993 |
| correlation between worm burden and the incidence of circulating anodic antigens or antibodies in mice infected with schistosoma japonicum or schistosoma mansoni. | | 1988 | 3141678 |
| hla-typing in schistosoma japonicum infection. | | 1988 | 3142119 |
| [effects of praziquantel on the immunological response in mice infected with schistosoma japonicum]. | | 1988 | 3142211 |
| [effect of praziquantel on ultrastructure of schistosoma japonicum cercariae and schistosomula within mouse skin]. | | 1988 | 3143224 |
| molecular and serological characteristics of the glutathione s-transferases of schistosoma japonicum and schistosoma mansoni. | when aqueous extracts of schistosoma japonicum and s. mansoni adult worms are passed over columns of glutathione-conjugated agarose, two molecular species of mr 26,000 and mr 28,000 are detected in eluates as analysed by sds-page, these eluates having glutathione s-transferase (gst) activity. the molecules, termed sj26 and sj28 from s. japonicum and sm26 and sm28 from s. mansoni, can be immunogenic in rabbits or mice and appear not to be linked together as subunits of gst heterodimers. the eluti ... | 1988 | 3146049 |
| [study on circumoval precipitin test using dry egg antigen smear on pvc membrane in the diagnosis of schistosomiasis japonica]. | | 1988 | 3149552 |
| [a preliminary study on applicability of latex agglutination test in schistosomiasis-eradicated area]. | | 1988 | 3149555 |
| [diagnostic proteins of 31/31 kd in schistosoma japonicum: reactivity with patient sera and monoclonal antibodies]. | | 1988 | 3150946 |
| [the reconformation of the existence of lithoglyphopsis modestus (gredler, 1886) in hunan province, china and its artificial infection with schistosoma japonicum]. | | 1988 | 3150947 |
| [a computer programme for automatic recognition of schistosome eggs]. | | 1988 | 3150948 |
| role of a mouse monoclonal ige antibody in passive transfer of immunity to schistosoma japonicum infection. | we have been able to produce a mouse monoclonal ige antibody specific to an adult worm antigen extracted from schistosoma japonicum (sj). the antibody was able to elicit passive cutaneous anaphylaxis in the rat skin against sj with the highest titer of 1:256,000 but did not cross-react with s. mansoni antigen. the antibody recognized a 97-kda molecule expressed on the surface of mechanically transformed schistosoma of s. japonicum. passive transfer of the antibody into mice in the early stage of ... | 1987 | 3151097 |
| an outbreak of schistosomiasis japonica in taiwan. | the present study was undertaken to determine the current status of schistosoma japonicum infection in the newly found endemic focus at hanpao farm, fangyuan district, changhua county, west-central taiwan. two field surveys and one laboratory investigation were conducted from july to october 1987. of 705 snails examined by shedding and crushing methods, 17.0% were found to harbor cercariae of s. japonicum. by sodium sulfate-acid-ether-triton centrifugal sedimentation technique, 25% of 16 cattle, ... | 1988 | 3151482 |
| sensitization against the parasite antigen sj26 is not sufficient for consistent expression of resistance to schistosoma japonicum in mice. | mice immunized with purified antigen preparations produced in escherichia coli and containing the glutathione s-transferase (gst) isoenzyme of schistosoma japonicum (sj26) can be partially resistant to infection with this parasite. maximum resistance was approximately 50% and no protection was obtained in balb/c mice, known low responders to sj26. although only freund's complete adjuvant has been used, the data obtained indicate that satisfactory levels of resistance to s. japonicum will not be ... | 1988 | 3151530 |
| serological evaluation of ultrasound examination for chronic schistosomiasis japonica in a previously endemic area--the chikugo river basin, japan. | ultrasound diagnosis of chronic schistosomiasis japonica was assessed by comparison with the results of serological tests using enzyme-linked immunosorbent assay (elisa) with egg and adult worm antigen, and the circumoval precipitation test. the subjects were persons resident in the chikugo river basin in kyushu, southwest japan, where schistosomiasis used to be highly prevalent. of 93 individuals with a past history of infection, 37 (39.8%) presented the characteristic echo pattern of the disea ... | 1988 | 3151543 |
| monoclonal ige-dependent eosinophil cytotoxicity to haptenated schistosomula of schistosoma japonicum: enhancement of the cytotoxicity and expression of fc receptors for ige by nippostrongylus brasiliensis infection. | to obtain direct evidence for the involvement of ige antibodies in eosinophil-mediated killing of schistosomula of s. japonicum, dinitrophenylated (dnp) schistosomula pretreated with mouse monoclonal ige antibodies were co-cultured with purified rat peritoneal eosinophils. it was found that the eosinophil-mediated adherence and damage to haptenated schistosomula were dependent on a monoclonal anti-dnp ige antibody, but not on monoclonal anti-ovalbumin ige antibody. moreover, eosinophils from n. ... | 1985 | 3156188 |
| [effect of calcium, magnesium and temperature on praziquantel-induced tegument damage of the male schistosoma japonicum]. | | 1985 | 3158162 |
| regulation of egg antigen-induced in vitro proliferative response by splenic suppressor t cells in murine schistosoma japonicum infection. | beginning about 5 weeks after infection, c57bl/6j mice infected with schistosoma japonicum developed granulomas around parasite eggs trapped in the liver. these granulomas attained peak size about 9 weeks after infection and then spontaneously regressed. this regression was also induced by the injection of serum immunoglobulin g1 but not lymphoid cells from chronically infected mice, but it was conceivable that lymphoid cells from mice infected for 10 weeks could also induce regression. we inves ... | 1985 | 3161831 |
| schistosoma japonicum (chinese): changes of the tegument surface in cercariae, schistosomula and juvenile parasites during development. | | 1988 | 3220650 |
| high-molecular-weight eosinophil chemotactic factor from schistosoma japonicum eggs. | | 1988 | 3226304 |
| [isolation and identification of schistosoma japonicum messenger ribonucleic acid]. | | 1988 | 3242967 |
| studies on antigenic communities between the yamanashi and chinese strains of schistosoma japonicum eggs and oncomelania snails by immunoelectrophoresis. | | 1988 | 3243720 |
| [studies on adenylate cyclase in schistosoma japonicum]. | | 1988 | 3253028 |
| peculiarities of mekong schistosomiasis with particular attention to the people's democratic republic of laos. | the authors examined 10 children with clinical symptoms suggestive of intestinal or hepatolienal schistosomiasis. all the children were from the endemic focus in laos. we found eggs of schistosomes that were smaller and rounder (60.95 x 51.55 microns) than those from a southern chinese strain of s. japonicum (62.64 x 73.49 microns). there were no oncomelania species found among the snails in this endemic area. these observations confirm those reports in the literature that assert a new species f ... | 1988 | 3270583 |
| expression of an enzymatically active parasite molecule in escherichia coli: schistosoma japonicum glutathione s-transferase. | the nh2-terminal amino acid sequence of the mr 26 000 glutathione s-transferase (ec 2.5.1.18) of schistosoma japonicum (sj26) has been deduced by rna and protein sequence analysis. using this information, a bacterial plasmid has been constructed that directs the synthesis of the entire sj26 molecule in escherichia coli. recombinant sj26 exhibits glutathione s-transferase activity and can be readily purified from bacteria in a one-step procedure under non-denaturing conditions. the availability o ... | 1988 | 3278228 |
| schistosoma japonicum and paragonimus ohirai: antagonism between s. japonicum and p. ohirai in oncomelania nosophora. | antagonistic interactions between schistosoma japonicum and paragonimus ohirai were examined in the snail host, oncomelania nosophora. when p. ohirai-infected snails were exposed to s. japonicum miracidia at intervals of 4 to 18 weeks post-first exposure, only a few snails (0-7%) were found to be superinfected with s. japonicum sporocysts. sporocysts were fewer in number than those of single infected controls. mature s. japonicum cercariae were not observed. furthermore, when the snails were exa ... | 1988 | 3338543 |
| schistosoma malayensis n. sp.: a schistosoma japonicum-complex schistosome from peninsular malaysia. | schistosoma malayensis n. sp., a member of the schistosoma japonicum complex is described from rattus muelleri in peninsular malaysia and 2 strains are characterized. the only morphological differences noted among adults from natural hosts were that s. malayensis are in general smaller than s. mekongi and s. japonicum. but these differences may be the result of host-induced variations and therefore are of little taxonomic value. to minimize the effects of host-induced variations, adult worms rec ... | 1988 | 3379527 |
| immune responses in mice infected with schistosoma japonicum and treated with praziquantel in special reference to the role of egg antigen. | the sequential change of class-specific antibodies to antigens of the schistosoma japonicum egg and adult worm in mice after treatment with a single oral dose of praziquantel (pzq) at 100 mg/kg was studied using elisa in regard to the resulting release of egg antigen. in 3 out of 4 mice treated with pzq, only igm antibody to egg antigen was drastically declined from 30 min to 6 hr after treatment, and then gradually rose to the initial level after 2 to 3 days. after adding 100 micrograms/ml pzq ... | 1988 | 3411793 |
| differential susceptibility of male and female oncomelania hupensis quadrasi infected with schistosoma japonicum. | | 1987 | 3429119 |
| the simple laboratory maintenance of a highly productive schistosoma japonicum life cycle. | the maintenance and infection of oncomelania hupensis hupensis, the intermediate host of the chinese strain of schistosoma japonicum, is described. 60% of the snails exposed to miracidia became patent, and 5000 patent snails can be produced which yield over 250,000 cercariae per week. the maintenance and infection of snails require less than 8 man-hours per week. | 1987 | 3445324 |
| effect of praziquantel on larval stages of schistosoma japonicum. | the effect of praziquantel on s. japonicum mother sporocysts, daughter sporocysts and cercariae was studied. at concentrations of 3 x 10(-7), 3 x 10(-6) and 3 x 10(-5) m and treatment times of 24 or 48 h, mother and daughter sporocysts and young cercarial embryos were not affected but nearly mature cercariae were killed and dissociated. the resistance of young cercariae could support the suggestion that the primitive cercarial epithelium arises from the sporocyst tegument. treatment with praziqu ... | 1987 | 3445349 |
| [effect of praziquantel on schistosoma japonicum cercariae]. | | 1987 | 3445780 |
| [role of respiration in the energy metabolism of schistosoma japonicum]. | | 1987 | 3447776 |
| [effects of praziquantel on the tegument of different developmental stages of schistosoma japonicum and host cell response]. | | 1987 | 3447783 |
| scanning electron microscopic study of the tegumental surface of the hybrid schistosome between schistosoma mekongi and s. japonicum-like (malaysian). | hybridization experiments between the two non-sibling species of schistosomes, schistosoma mekongi in man and s. japonicum-like (malaysian) in rodents, were carried out. two laboratory-bred snail species, tricula aperta (beta race), the snail host of s. mekongi and robertsiella kaporensis, the snail host of s. japonicum-like (malaysian), were used for the production of cercariae. cross mating between s. mekongi and s. japonicum-like (malaysian) were achieved in the laboratory by the usual proced ... | 1987 | 3448774 |
| [effects of praziquantel in vitro on adherence of neutrophils to schistosomula and killing of the worms]. | | 1987 | 3451669 |
| praziquantel: mode of action. | | 1987 | 3493930 |
| identification of a particular antigen from a parasite cdna library using antibodies affinity purified from selected portions of western blots. | portions of nitrocellulose filters containing blotted electrophoresed antigens of schistosoma japonicum adult worms were reacted with polyclonal rabbit antisera raised to this human parasite. eluted antibodies were used as probes for detection of antigen-positive clones in an escherichia coli lambda gt11 amp3 expression library of adult worm cdna. several cloned antigens corresponding to a s. japonicum antigen of mr 26 000, being sought as a candidate vaccine molecule in a mouse model of schisto ... | 1986 | 3511153 |
| expression of schistosoma japonicum antigens in escherichia coli. | a cloned library of dna complementary to the mrna of adult schistosoma japonicum has been prepared and expressed as fusion proteins with escherichia coli beta-galactosidase. colonies expressing the s. japonicum cdna clones were screened both with antibodies from individuals with a history of schistosomiasis and with antibodies obtained from a rabbit immunized with whole adult worms. in both cases colonies were detected which bound antibody, although the frequency of antigen-positive clones was m ... | 1986 | 3515179 |
| schistosoma japonicum: ultrastructural localization of a hemoglobinase using mercury labeled pepstatin. | mercury labeled pepstatin was used to demonstrate the site of a pepstatin sensitive hemoglobinase in paraformaldehyde fixed adult schistosoma japonicum. pepstatin was covalently attached to glutathione using dicyclohexylcarbodiimide followed by addition to methyl mercury chloride. deposition of mercury was observed in lipid-like globules and autophagic vacuoles in the gastrodermis. control studies were negative in all instances. these results complement previous cytochemical studies on the distr ... | 1986 | 3527739 |
| the phospholipid and fatty acid composition of schistosoma mansoni and of its purified tegumental membranes. | the lipid compositions of mature male and female schistosoma mansoni and cercariae were compared to that of the hepato-pancreas of unparasitised biomphalaria globrata (the intermediate snail host), and of red blood cells and sera of hamsters (the mammalian host). membranes were isolated from the tegument of mature schistosomes by spontaneous release into phosphate-buffered saline, with or without vortexing, and by removal from the parasite's surface using poly-lysine beads. the phospholipid comp ... | 1987 | 3574354 |
| [studies on the synthesis of salicylamides and alkamine salts of niclosamide and their activities against schistosoma japonicum and oncomelania]. | | 1986 | 3591335 |
| host identification by schistosoma japonicum cercariae. | attachment, the first phase of host identification by schistosoma japonicum cercariae, can occur in 2 different ways. cercariae clinging to the water surface simply swing around and transfer to the host skin. free-swimming cercariae behave like s. mansoni: upon touching a substrate, they switch from tailward to forward movement, swim in an arc, and attach to it with the penetration organ. neither type of attachment is influenced by chemical, thermal, or specific mechanical stimuli from the host. ... | 1987 | 3598807 |
| uptake and excretion of amino acids and utilization of glucose by schistosoma japonicum eggs. | the utilization of amino acids and glucose in the external nutrients and the excretion of nitrogenous compounds by schistosoma japonicum eggs were investigated with the eggs cultured in a chemically defined medium (memse-j). of the 15 amino acids in memse-j, arginine and glutamine markedly decreased in concentration during cultivation of s. japonicum eggs. the nitrogenous excretory products of developing eggs were demonstrated to be at least four amino acids (alanine, proline, glutamic acid and ... | 1986 | 3599527 |
| [observation on the difference in susceptibility of oncomelania snails to different isolates of schistosoma japonicum]. | | 1987 | 3621506 |
| [cell lines of hybridoma secreting monoclonal antibodies against schistosoma japonicum egg antigens]. | | 1987 | 3621514 |
| degenerative changes in the reproductive organs of female schistosomes during maintenance in vitro. | degenerative alteration of the reproductive organs of female schistosomes in correlation with the change in egg-laying rate of schistosome pairs in vitro was studied by electron microscopy. the production of normal eggs by adult s. japonicum pairs decreased after 4 days in vitro followed by an increase of abnormal egg laying up to day 8. in s. mansoni, the yield of both normal and abnormal eggs decreased gradually from the start of maintenance in vitro in spite of a much higher pairing rate than ... | 1987 | 3625433 |
| large genetic distance between chinese and philippine schistosoma japonicum. | | 1987 | 3625440 |
| [conversion of schistosoma japonicum cercariae to schistosomula under the action of praziquantel]. | | 1987 | 3652413 |
| comparative glucose utilization rates in separated and mated schistosomes. | the rate of phosphorylation of 2-deoxyglucose (2dg) was determined by sequential pulsing of schistosomes (schistosoma mansoni, s. japonicum, and s. haematobium) with 3h- and 14c-labeled 2-deoxy-d-glucose. subsequent column chromatographic separation of the neutral [3h]2dg and [14c]2dg from the 3h- and 14c-labeled 2-deoxy-d-glucose 6-phosphate permitted estimation of the quantity of [3h]2dg phosphorylated in 2 min, and the proportion of [14c]2dg phosphorylated in 1 min; thus a phosphorylation rat ... | 1987 | 3678450 |