| effect of hamster liver passage on the isoenzyme patterns of entamoeba histolytica. | the effect of hamster liver passage on the isoenzyme patterns of isolates of entamoeba histolytica was investigated. three isolates, f, g and t were originally obtained from patients with acute amebic dysentery and another strain, c, was obtained from an asymptomatic carrier. all these strains were maintained for over two years in axenic culture. the isoenzyme pattern (zymodeme) of hexokinase (hk), phosphoglucomutase (pgm) and glucose phosphoisomerase (gpi) of these strains was found to belong t ... | 1992 | 1304461 |
| amebiasis: a problem solved. what now? bernardo sepúlveda memorial lecture. | | 1992 | 1308698 |
| local and systemic antibody response in balb/c mice immunized with entamoeba histolytica trophozoites. | several immunization schedules with e. histolytica trophozoites were tested on balb/c mice in order to induce antibody responses, both in intestinal secretions and in serum. mice were immunized either orally, systemically, or using one of two combined schedules: the oral route followed by the systemic route (footpad), or vice versa. each of the immunization schedules used in this project induced an anti-e. histolytica antibody response and there appears to be a correlation between the immunizati ... | 1992 | 1308795 |
| chemotherapy and immunity in opportunistic parasitic infections in aids. | parasitic diseases are endemic in parts of the tropics, but there is no convincing evidence that their prevalence or incidence is increasing due to the hiv epidemic. available scientific data on parasitic infections in patients with the acquired immunodeficiency syndrome (aids) suggests a predominance of pneumocystis carinii, toxoplasma gondii and cryptosporidium spp. for reasons which are unclear, parasitic infections such as plasmodium falciparum, strongyloides stercoralis and entamoeba histol ... | 1992 | 1308934 |
| immunochemical characterisation of a 29-kda surface-associated molecule of entamoeba histolytica and its recognition by serum from patients with amoebiasis. | a 29-kda cytotoxic molecule of axenically-grown pathogenic entamoeba histolytica (strain hm1) was purified from an amoebic extract by immuno-affinity chromatography with monoclonal antibodies. immunoreactivity of the purified 29-kda molecule altered significantly (p less than 0.01) after exposure to heat or trypsin, but remained unaltered after treatment with sodium metaperiodate. the 29-kda molecule was recognised by serum from each of 13 patients with amoebic liver abscess. in an elisa system, ... | 1992 | 1309884 |
| macrophage cytotoxicity against entamoeba histolytica trophozoites is mediated by nitric oxide from l-arginine. | the killing of entamoeba histolytica trophozoites by phagocytes involves oxidative and nonoxidative mediators. in this study, we determine whether l-arginine-derived nitric oxide (no) is involved in the killing of e. histolytica trophozoites by activated murine macrophages in vitro. elicited peritoneal and bone marrow-derived macrophages activated with ifn-gamma alone or with ifn-gamma and lps killed 62 to 73% of amebae, concomitant with increased levels of nitrate (no2). depletion of l-arginine ... | 1992 | 1318338 |
| ubiquitin of entamoeba histolytica deviates in six amino acid residues from the consensus of all other known ubiquitins. | the amino acid sequence of ubiquitin from entamoeba histolytica, as deduced from a cdna nucleotide sequence, deviated at six positions from the consensus of all other known ubiquitins (ranging from trypanosoma cruzi to homo sapiens). the corresponding residues were scattered over the primary sequence, but came close together on the surface of the folded protein structure. we conclude that (i) e. histolytica branched off very early from the main eukaryotic line, and (ii) this organism may yield c ... | 1992 | 1322840 |
| [electron microscopic enzyme cytochemistry of entamoeba histolytica trophozoite]. | electron microscopic enzyme cytochemical reactions of entamoeba histolytica trophozoite showed that acid phosphatase (acp) and cytidine monophosphatase (cmpase) were located in the lysosomes. the lysosome containing enzymes were distributed in the endoplasm and beneath the plasmalemma, and the releasing enzymes by lysosomes excreted outside of the plasmalemma and caused the injury to host cells. the cytochemical positive reactions of catalase and glucose-6-phosphatase (g-6-pase) showed that e. h ... | 1992 | 1337724 |
| recent progress in the molecular biology of entamoeba histolytica. | entamoeba histolytica, a protozoan parasite causing human amoebiasis, has recently been found to comprise two genetically distinct forms, potentially pathogenic and constitutively nonpathogenic ones. host tissue destruction by pathogenic forms is believed to result from cell functions mediated by a lectin-type adherence receptor, a pore-forming peptide involved in host cell lysis, and abundant expression of cysteine proteinases. comparisons of these products from pathogenic and nonpathogenic e. ... | 1992 | 1338130 |
| entamoeba histolytica: phylogenetic considerations. a review. | comparison of 16s rrna sequences alone positions e. histolytica in rrna-based phylogenies branching after flagellates such as euglenoids and kinetoplastids, whereas morphological and functional features had suggested an earlier branching and different relationships. as several characteristics of this parasite do not precisely adjust to the phylogenetic frame obtained by comparison of ribosomal sequences, and the inferences from this approach could be skewed because of the high a+t content of ent ... | 1992 | 1340267 |
| subcellular distribution of amebapain, the major cysteine proteinase of entamoeba histolytica. | amebapain, the major cysteine proteinase of e. histolytica, appears to be important both for digestion and as a cytotoxic factor. by immunocytology we established that amebapain was bound to matrix-like structures both on the cell surface and within subcellular vesicles; the amebapain co-localized with pinocytized iron oxide, suggesting that the enzyme recycles between plasma membrane and pinocytic vesicles. we conclude from these data that (i) the pinocytic vesicles in e. histolytica perform fu ... | 1992 | 1340268 |
| entamoeba histolytica collagen binding proteins. | three main collagen binding proteins from trophozoites of e. histolytica have been isolated: 105 kda, 56 kda and 30 kda. they display a type i collagenolytic activity. the enzyme behaves as a mammalian collagenase regarding to its activity, collagen degradation products and inhibitors. antibodies against affinity-purified molecules inhibit the binding of trophozoites to collagen substrates. indirect evidence suggests that the 30 kda molecule is the putative collagen receptor. these surface molec ... | 1992 | 1340269 |
| collagenase activity in clinical isolates of entamoeba histolytica maintained in xenic cultures. | several isolates of pathogenic and non-pathogenic e. histolytica from xenic cultures were tested for their capacity to digest native type i collagen gels. the results demonstrate that the pathogenic isolate hm48:imss has a high collagenolytic activity. the non-pathogenic isolates, hm43:imss, hm44:imss and hm46:imss showed significantly lower collagenolytic activity. six groups showing different degrees of collagenase activity or non-activity were separated from the non-pathogenic isolates by a p ... | 1992 | 1340271 |
| identification and partial purification of an entamoeba histolytica membrane protein that binds fibronectin. | a 37 kda protein has been described as a putative receptor for fibronectin (fn) on e. histolytica trophozoites (1). we have now identified a membrane protein that binds biotinylated fibronectin (bfn) with an apparent molecular weight of 140 kda. using bfn we were able to follow this protein during partial purification through deae-cellulose and fn-sepharose chromatography. antisera prepared against a peptide corresponding to the deduced amino acid sequence for the putative receptor binding site ... | 1992 | 1340272 |
| fibronectin "receptor" in entamoeba histolytica: purification and association with the cytoskeleton. | preferential binding of e. histolytica trophozoites to fibronectin (fn) is mediated by a 37 kda peptide (1). we report now the further purification of this "putative" fn receptor (fn-r) and its characterization as an external membrane component also associated to the cortical cytoskeleton. the fn-r was solubilized from triton-cytoskeletons and separated by chromatography in fn-sepharose. the fn-r, labeled when live trophozoites were iodinated with 125i, remained associated to the cytoskeleton to ... | 1992 | 1340273 |
| actin associated proteins of entamoeba histolytica. | treatment of e. histolytica hm1-imss trophozoite extracts to conditions that produce gels of actin and associated cytoskeletal proteins in other ameboid cells caused formation of macroscopic actin rich complexes (arcs). the one-dimensional page protein profile of this arc was similar to those of dictyostelium and acanthamoeba actin gels. formation of the e. histolytica arcs was enhanced by added lipids. in addition to actin, the arc was enriched with proteins that showed cross-reactivity to anti ... | 1992 | 1340274 |
| inhibition of the complement membrane attack complex by the galactose-specific adhesin of entamoeba histolytica. | | 1992 | 1340275 |
| biochemical analysis of entamoeba histolytica hm1 strain resistant to complement lysis. | in this study, quantitation of several hydrolases and analysis of electrophoretic patterns of e. histolytica hm1 axenic strain periodically exposed to lytic-human serum (lytic-hs), heat-inactivated human (hi-hs) or bovine (hi-bs) sera were performed. the n-acetylglucosaminidase (nagase) levels increased in amebas resistant to complement. the acid phosphatase (ap) and proteolytic activity (pa) levels did not vary significantly in all groups of treated amebas, as compared with amebas from axenic c ... | 1992 | 1340276 |
| macrophage colony-stimulating factor enhances the respiratory burst of human monocytes in response to entamoeba histolytica. | mononuclear phagocytes (mp) are probably the most capable effector cells of the body in the defense against virulent strains of e. histolytica. killing of e. histolytica by mp appears to involve both oxidative and non-oxidative mechanisms. thus, in this study we have investigated whether trophozoites of an axenic virulent strain e. histolytica hm1:imss (eh) were capable of eliciting an oxidative response in pure populations of freshly isolated human monocytes. using a luminol-enhanced chemilumin ... | 1992 | 1340277 |
| the killing of entamoeba histolytica by activated human eosinophils. | unlike normal, opsonin aided human eosinophils, fmlp-activated human eosinophils are capable of destroying virulent e. histolytica. opsonins are not required for this action, although they enhance the effect. some activated eosinophils succumb in the action as well, probably victims of toxic products released by dying amebas. activated eosinophils thus appear to resemble activated macrophages in their dealing with this parasite. | 1992 | 1340278 |
| entamoeba histolytica modulates tnf-alpha, il-1 alpha/beta and c-fos gene expression in macrophages. | e. histolytica infections induce a state of transient suppression of cell-mediated immunity. as macrophages are involved in host defense in amebiasis, we determined whether soluble amebic lysates (eh) can modulate tnf-alpha, il-1 alpha/beta and c-fos gene expression in naive bone marrow-derived macrophages (bm delta). by northern analysis, the rna production of these genes after 0, 0.5, 1 and 3 h exposure to eh was determined and compared to lipopolysaccharide (lps) stimulation. in response to e ... | 1992 | 1340279 |
| further physicochemical characterization of the monocyte locomotion inhibition factor (mlif) produced by entamoeba histolytica. | ultrafiltration, gel-sieve chromatography and hplc were used to purify mlif. this material was hcl-hydrolyzed and the amino acids analyzed by hplc-ortho-ophthaldialdehyde. two hydrophobic non-polar (ile,leu), two uncharged polar (thr,tyr) and two positively charged (basic) (his, arg) amino acids were found in clear excess to their concentration in amc and thus may intervene in the composition of mlif. | 1992 | 1340280 |
| sex differences in systemic and local immune responses to entamoeba histolytica after intraperitoneal and rectal immunization in balb/c mice. | the antibody responses against entamoeba histolytica in peyer's patches and spleen after rectal, intraperitoneal and intragastric immunization with glutaraldehyde-fixed amebas (gfa) was compared between male and female mice by the use of a spot forming cell (sfc) assay. we found that female mice elicit significant higher anti-amebic sfc responses than male mice, in both spleen and peyer's patches after rectal and intraperitoneal stimulation, whereas by intragastric route females show higher resp ... | 1992 | 1340281 |
| effect of the monocyte locomotion inhibitory factor (mlif) produced by entamoeba histolytica upon the respiratory burst of human leukocytes. | in addition to inhibiting the locomotion of human mp, mlif appears capable of inhibiting the respiratory burst (measured by chemiluminescence) of mp and of pmn as well. the effect on the latter cells may or may not be relevant in the host-e. histolytica interaction, as pmn have been found to be notoriously inefficient in dealing with amebas and, foremost, do not use oxidative mechanisms in dealing with the parasite. on the other hand, the inhibitory effect on mp may represent a true evasion mech ... | 1992 | 1340282 |
| active immunization in hamsters with live entamoeba histolytica trophozoites of low virulence. | live trophozoites of entamoeba histolytica of low virulence (lv) were inoculated intraportally in hamsters. one week later they were challenged intrahepatically with highly virulent (hv) trophozoites of the same strain and sacrificed 1 week later. significant protection manifested by the production of smaller lesions was observed in immunized animals. antiamebic igg levels were significantly higher in the immunized group. histologically, livers of immunized animals presented small lesions contai ... | 1992 | 1340283 |
| kinetics of the anti-amebic antibody producing cells response in peyer's patches and spleen after both local and systemic stimulation in balb/c mice. | the kinetics of the anti-amebic antibody producing cell (apc) response was analyzed by a spot forming cell (sfc) assay, after a single dose of glutaraldehyde fixed amebas (gfa) by intragastric, rectal and intraperitoneal routes. it was found that mice elicited both local and systemic immune responses in peyer's patches and spleen, by either local or systemic stimulation. the analysis of the isotype of the anti-amebic apc in mice given four times the gfa by the mentioned routes revealed that mice ... | 1992 | 1340284 |
| identification of a parasite-specific cytoplasmic 67 kda entamoeba histolytica antigen recognized by patient sera and monoclonal antibodies. | to avoid false positive reactions in tests for anti-ameba antibodies, we wanted to identify parasite-specific component(s). amebiasis patient sera recognized an antigen of 67 kda by immunoblotting in an active e. histolytica fraction obtained by ion exchange chromatography. monoclonal antibodies against the fraction were made. antibody 3g2 reacted with three antigenic components of 67, 40 and 25 kda and in the immunocytology with an epitope located in the cytoplasm of e. histolytica trophozoites ... | 1992 | 1340285 |
| scanning electron microscopy of erythrophagocytosis by entamoeba histolytica trophozoites. | we analyzed by scanning electron microscopy (sem) the sequence of erythrocyte ingestion by a virulent strain of entamoeba histolytica trophozoites. axenic cultures of amebas were placed in chamber plastic slides for 30 min. human red blood cells (rbc) were added to the wells in a ratio of 1:100, ameba:erythrocytes. after different times of interaction, cells were fixed and processed for sem. erythrophagocytosis by e. histolytica is characterized by random suction of cells at early stages and pol ... | 1992 | 1340286 |
| phagocytosis and proteinase activity are not related to pathogenicity of entamoeba histolytica. | to examine the relationship between phagocytosis, proteinase activity and pathogenicity of axenically grown trophozoites of e. histolytica of strain hm-1:imss four different cultures were used: 1) a culture preserved in our laboratory for over 4 years, that lost its pathogenicity 3 years ago; 2) a culture passaged several times through hamster liver, that lost its pathogenicity recently; 3) a highly virulent culture supplied by another laboratory; and 4) amebas recovered from hamster liver absce ... | 1992 | 1340287 |
| a unique cysteine proteinase gene of pathogenic entamoeba histolytica correlates with virulence. | extracellular neutral cysteine proteinases are an important virulence factor of e. histolytica. experimental evidence supporting its role in invasion includes the ability to degrade components of the extracellular matrix and activate complement by specifically cleaving c3. we had previously reported the isolation of fragments encoding cysteine proteinase genes from hm-1 (acp1) and a nonpathogenic strain (ref291, acp2) by pcr using consensus sequences based on conserved structural motifs of eukar ... | 1992 | 1340288 |
| effect of intestinal bacteria on the virulence of entamoeba histolytica. | the effect on amebal virulence of escherichia coli, clostridium symbiosum and a mixture of lactobacilli was investigated. amebas from hm1 and hk axenic strains were incubated with a single bacterial strain or lactobacilli for short, intermediate, or long periods and analyzed for their erythrophagocytosis, hemolytic activity and cytotoxicity in vitro and hepatic abscesses formation in vivo. it was found that in vitro virulence was enhanced by all studied bacteria in hk9 but not in hm1 strains, wh ... | 1992 | 1340289 |
| cdna cloning of entamoeba histolytica histone h3. | e. histolytica has an unusual nuclear structure. although nucleosome-like particles were observed, no information about histones was available so far. in this paper we describe a cdna clone with significant homology to h3 histones that was isolated from a lambda zap library of pathogenic e. histolytica. the complete cdna encodes a 15 kda polypeptide, which is shorter by one residue than the human homologue. the amino acid sequence has only 69% identity with human h3.3 histone. our results indica ... | 1992 | 1340291 |
| immediate cell membrane damage produced by entamoeba histolytica subcellular extracts. | the alpha[14c]amino isobutyric acid is a synthetic amino acid with low molecular weight, not incorporated to protein. chinese hamster ovary (cho) cells were labeled with this radioactive compound and treated with the amebal subcellular fraction p30 at short times. we detected membrane damage in cho cells by specific radiolabel release from the first 30 sec of interaction between cell cultures and amebal fraction. | 1992 | 1340292 |
| interaction of various entamoeba histolytica strains with human intestinal cell lines. | the interaction of four pathogenic and three nonpathogenic e. histolytica strains with two human intestinal cell lines (caco-2 and ht-29) was examined. the adherence of pathogenic and nonpathogenic e. histolytica to these cells was similar, indicating that defective adherence to intestinal cells is not a common feature of nonpathogenic strains. the addition of different carbohydrates confirmed the importance of the galactose-binding lectin of e. histolytica in binding to these intestinal cells. ... | 1992 | 1340293 |
| model of intestinal amebiasis: structural and functional lesions to the rabbit colon mucosa by entamoeba histolytica lysates. | although the events occurring during the initial interaction of e. histolytica trophozoites with the mucosa of the large intestine probably determine the invasion by the parasites, an appropriate experimental model does not exist. to develop such a model we used full-thickness rabbit colon preparations (0.28 cm2) mounted in ussing-type chambers. untreated preparations had electrophysiological properties (potential difference, short-circuit current and electrical resistance) similar in magnitude ... | 1992 | 1340294 |
| morphological characterization of experimental amebic liver lesions in gerbils. | gerbils are susceptible to amebic liver abscess (ala) production under experimental conditions. however, little is known about the histopathological changes that occur during the evolution of the lesion. we analyzed microscopically the sequence of cellular events of ala in gerbils inoculated intraportally or intrahepatically with e. histolytica. at early stages trophozoites were associated with different degrees of inflammatory reaction. amebas were also observed in areas devoid of inflammatory ... | 1992 | 1340295 |
| histological changes during healing of experimental amebic liver abscess treated with metronidazole. | morphological changes during the healing of amebic liver abscess (ala) in hamsters treated with metronidazole were studied. animals with ala of 4 days of evolution were treated orally with metronidazole (5 mg/100 g for 10 days). liver samples were studied at different times from the beginning of treatment up to 22 days. the resolution of ala was accompanied by chronic inflammatory reaction, increased number of foamy macrophages and gradual disappearance of the granulomas. neoformed hepatocytes a ... | 1992 | 1340296 |
| growth inhibition of entamoeba histolytica by rat colon components. | susceptibility to invasive amebiasis has been suggested to be due to intrinsic amebic factors and/or to such host factors as intestinal microflora, mucus and colonic redox potential. we investigated the effect of rat colon components on the growth of axenically cultured e. histolytica trophozoites. extracts of rat colon tissue produced a 57% amebic growth inhibition. the main growth inhibitory components were precipitated by 65% ammonium sulfate and were heat-sensitive. these components were par ... | 1992 | 1340297 |
| the fast release of mucin secretion from human colonic cells induced by entamoeba histolytica is dependent on contact and protein kinase c activation. | the mucus producing colonic cell line, ls174t, was used as a model to study e. histolytica-induced mucin secretion. e. histolytica trophozoites in contact with the mucus layer overlying the ls174t cells and in response to pma, a protein kinase c activator, and ca2+ ionophore a23187 which elevates intracellular ca2+ ([ca]i), caused a time-dependent (0.25-2.00 h) release of mucin. pkc inhibitors, h7 and staurosporine inhibited e. histolytica (37 and 75%) and pma (46 and 100%)-induced mucin secreti ... | 1992 | 1340298 |
| bile salts promote adherence-decreasing effect of colonic luminal hydrolases on entamoeba histolytica. | e. histolytica trophozoites cultivated for > 7 h in the presence of glycosidases, produced by a subset of the colonic anaerobic bacteria of healthy humans, and pancreatic proteases show decreased adherence to chinese hamster ovary epithelial cells. since activities of the glycosidases are enhanced by bile salts we investigated whether bile salts would enhance the e. histolytica-cho cell adherence decreasing effects of the luminal hydrolases (glycosidases plus proteases). cho cell adherence of co ... | 1992 | 1340299 |
| inhibition of adhesion process mediated by anti-entamoeba histolytica specific monoclonal iga antibodies. | adhesion of trophozoites to target cells in the host intestine is the first of three consecutive steps (adherence, cytolytic effect and phagocytosis) involved in the invasion of colonic tissue by e. histolytica. to investigate the possible participation of the local secretory immune response in the interference with this early host-parasite relationship, we produced iga monoclonal anti-e. histolytica antibodies to test their capacity for blocking the adhesion process in vitro (mdck and ht-29 cel ... | 1992 | 1340300 |
| neutral proteinase activities in different strains and clones of entamoeba histolytica. correlation with virulence. | although several factors are involved in the invasive behavior of e. histolytica, proteinases seem to play a key role. different proteinases have been found in virulent trophozoites of this parasite. cytosols of clones a, 32-1 462-1 and l-6 of e. histolytica exhibiting various degrees of virulence were used to study the activity of trypsin-like, plasminogen activator and cathepsin b neutral proteinases with specific synthetic oligopeptides. cathepsin-b like activity showed the highest values in ... | 1992 | 1340301 |
| inhibition of adhesion by monoclonal antibody to 66 kda surface antigen of entamoeba histolytica. | the adherence of e. histolytica trophozoites to target cells was studied using a monoclonal antibody to a major surface antigen of 66 kda. preincubation of trophozoites with monoclonal antibody decreased their ability to adhere to and engulf erythrocytes and destruction of cho cells. the monoclonal antibody was specific for the 66 kda antigen which is possibly a major participant in adhesion which precedes other events in phagocytosis and cytopathic effects. | 1992 | 1340302 |
| purification and functional characterization of the 112 kda adhesin of entamoeba histolytica. | the 112 kda adhesin of e. histolytica is directly involved in the cytopathogenic activity of the parasite. we describe here the purification of the 112 kda protein by electroelution and immunoaffinity chromatography using a monoclonal antibody against the adhesin. two proteins of 70 and 50 kda were eluted from the immunoaffinity column along with the 112 kda adhesin. the three proteins were recognized by monospecific polyclonal antibodies against the adhesin. the same peptides (72 and 56 kda) we ... | 1992 | 1340303 |
| purification and partial characterization of an hemolytic activity from entamoeba histolytica. | it is generally accepted that hydrolytic and cytolytic amebic components are involved in the pathogenic mechanisms of e. histolytica. we have now identified a lytic activity in two membrane proteins of 23.5 kda and 25 kda, which are able to lyse rat erythrocytes. the activity was purified from total homogenates of the virulent strains hm1:imss and hm38:imss, and the erythrocyte lysis was directly related to protein concentration. the hemolytic activity was heat-sensitive and resistant to reducti ... | 1992 | 1340304 |
| early detection of complications in amebic liver abscess. | a retrospective analysis of 140 cases with amebic liver abscess (ala) seen at the aunl university hospital was done to see if patients with complications can be identified earlier in order to decrease morbidity and mortality. sixteen patients (11.4%) presented complications and six patients died (4.2%). patients with complications presented jaundice, large or multiple abscesses, acute abdomen, liver failure and sepsis more often than patients without complications. hemoglobin, hematocrit, prothr ... | 1992 | 1340306 |
| molecular characterization of igg and ige antigens of entamoeba histolytica. | several surface igg antigens of e. histolytica have been characterized. we isolated two novel cdnas by screening a library of pathogenic e. histolytica with rabbit antiserum against a membrane preparation of pathogenic amebas. cdna k15 encodes a large protein > 220 kda with sequence homology to myosins and k18 encodes a protein of around 40 kda. serum igg from patients suffering from amebiasis binds to both antigens. some ige antibodies are able to recognize homologous proteins in all eukaryotes ... | 1992 | 1340307 |
| iga reactivity in patients with intestinal or hepatic amebiasis. | the antigens of e. histolytica recognized by iga antibodies in sera from patients with either amebic liver abscess or intestinal amebiasis, as well as saliva from the latter, were determined in immunoblots of whole native trophozoite proteins. results show that sera of patients with amebic liver abscess reacted mainly with amebic proteins of more than 200 kda, whereas those of individuals with intestinal amebiasis recognized a protein of 145 kda. the saliva of the latter detected a protein of 21 ... | 1992 | 1340308 |
| amebiasis in nicaragua: class specific serum antibody responses. | with the aid of the indirect hemagglutination (iha) test and igg elisa the antibody profile against e. histolytica in león, nicaragua was investigated in 562 sera from individuals belonging to various age groups. the highest reactivity was invariably recorded in the age group 6-15 years where 48% were seropositive. several sera reactive by either one of iha and igg elisa were negative by the other test. the main reason for this seems to be reactivity in different ig classes. treatment with 2-mer ... | 1992 | 1340309 |
| a survey of entamoeba histolytica and entamoeba dispar (brumpt) infections on mahé, the seychelles. | e. histolytica, pathogenic, and e. dispar, non-pathogenic, being morphologically identical are, when recovered as cysts in feces, diagnosed as e. histolytica. a survey on the seychelles demonstrated that when culture and zymodeme characterization was used to compare against microscopy alone, the risk of overdiagnosis of e. histolytica infections was drastically reduced. from a total of 313 subjects tested 21 cultures grew amebas. by zymodeme analysis eight were e. histolytica, 40 were e. dispar ... | 1992 | 1340310 |
| recombinant expression, purification and biochemical characterization of a superoxide dismutase from entamoeba histolytica. | a recombinant iron-containing superoxide dismutase (recfesod) of entamoeba histolytica was produced in a prokaryotic expression system. purified recfesod was found to be enzymatically active as determined by (i) inhibition of ferri-cytochrome c reduction, (ii) dismutation of superoxide anions generated by human neutrophils and (iii) inhibition of nitroblue tetrazolium reduction. the enzymatic properties of recfesod were similar to those of the native protein in trophozoite extracts. in an elisa ... | 1992 | 1340312 |
| a dna probe specific to pathogenic entamoeba histolytica. | a dna sequence, ie-gen1 (3.1 kb), was isolated from the pathogenic strain of e. histolytica nih-200. ie-gen1 was identified by the subtractive hybridization of a genomic library to a cdna probe prepared from nih-200 trophozoites. the ie-gen1 probe specifically detected pathogenic e. histolytica in slot blots of genomic dna and northern blots, but not other entamoeba species and additional human parasites. this genomic probe could detect with complete specificity dna from about 10(3) organisms. t ... | 1992 | 1340313 |
| use of polymerase chain reaction and nonradioactive dna probes to diagnose entamoeba histolytica in clinical samples. | e. histolytica parasites in mexican children's stools were identified and typed as pathogenic or non-pathogenic using the polymerase chain reaction (pcr) and nonradioactive probes. pcrs were performed with primers specific for 145 base pair (bp) pathogenic or 133 bp non-pathogenic dna sequences, which are highly repeated in e. histolytica parasites with pathogenic or non-pathogenic isoenzyme patterns, respectively. dot-blotted pcr products were identified with a horseradish peroxidase-conjugated ... | 1992 | 1340314 |
| further diagnostic use of an invasive-specific monoclonal antibody against entamoeba histolytica. | genotypic differences between invasive and non-invasive e. histolytica could explain the 1:10 ratio of symptomatic/asymptomatic infection worldwide. currently, zymodeme analysis is used to differentiate invasive from non-invasive e. histolytica strains but the technique is cumbersome and expensive. in accordance with the who research priorities for amebiasis we report here the further use of an invasive-specific monoclonal antibody against e. histolytica in immunofluorescence, to identify isolat ... | 1992 | 1340315 |
| serodiagnosis of invasive amebiasis using a recombinant entamoeba histolytica antigen-based elisa. | amebic serology remains a critical component in the diagnosis of invasive amebiasis. a recombinant serine rich-entamoeba histolytica-protein/maltose binding protein (srehp/mbp) fusion protein was evaluated as the target antigen in an elisa test to detect acute invasive amebiasis. retrospective analysis of 65 serum samples from patients with amebic liver abscess and 40 asymptomatic control patients showed the srehp/mbp elisa test had a sensitivity of 74% and specificity of only 55% for the diagno ... | 1992 | 1340316 |
| low risk of invasive amebiasis in cyst carriers. a longitudinal molecular seroepidemiological study. | a seroepidemiological study of a household cohort, using both clinical observational and molecular criteria was conducted in a periurban area endemic for e. histolytica infection. this longitudinal study was undertaken to determine the risk of asymptomatic cyst carriers to develop invasive illness. zymodeme patterns of strains isolated from these patients were correlated both with the clinical presentation of disease and with the serological response against the m-17 ameba antigen and further co ... | 1992 | 1340317 |
| primary structures of alcohol and aldehyde dehydrogenase genes of entamoeba histolytica. | ethanol is the major metabolic product of glucose fermentation by the protozoan parasite e. histolytica under the anaerobic conditions found in the lumen of the colon. with the goal of finding new targets for anti-amebic drugs, the e. histolytica nadp(+)-dependent alcohol dehydrogenase gene (ehadh1; ec 1.1.1.2) and an aldehyde dehydrogenase gene (ehaldh1; ec 1.3.2) were cloned. the ehadh1 alcohol dehydrogenase gene encoded -39 kda protein with 62 and 60% amino acid identities, respectively, with ... | 1992 | 1340318 |
| partial nucleotide sequence of the enzyme pyruvate, orthophosphate dikinase of entamoeba histolytica hm1:imss. | we isolated a clone from a lambda gt11 cdna library of e. histolytica, subcloned its insert in the vector ptz18r and sequenced it by the method of sanger. sequence comparison with genbank and of the corresponding amino acid sequence with the nbrf/pir data bank using the fasta program, showed homologies between 54.9 and 58.5% for the nucleotides and between 48.6 and 49.4% for the amino acids with pyruvate, orthophosphate dikinases from maize, flaveria trinervia and bacteroides symbiosus. the sequ ... | 1992 | 1340319 |
| identification of a myosin heavy chain gene from entamoeba histolytica. | the myosin heavy chain gene a, mhca, from e. histolytica was identified by polymerase chain reaction. there is only one copy of this gene on the ameba genome. the mhca gene encodes a 6 kb messenger rna. the predicted protein sequence is homologous to myosin ii from both, dictyostelium discoideum and acanthamoeba castellanii. specific areas of the n-terminal region of myosin ii are highly conserved reflecting a similarity in functional domains, like the atp or the actin binding sites. | 1992 | 1340320 |
| chromosome walk in entamoeba histolytica: the gene encoding for ribosomal protein l21 neighbors one of the actin genes. | actin is one of the most abundant proteins in the motile intestinal protozoan parasite e. histolytica. a number of actin gene copies have been detected. the cdna and genomic sequences of two of the actin genes have been independently reported (1,2). almost complete homology was detected between the coding regions of the two genes; however, significant differences were observed in the sequences of their 5' untranslated regions. using the coding region of actin as the focal point, we performed a c ... | 1992 | 1340321 |
| axenic massive cultivation of entamoeba histolytica trophozoites in spinner flasks and in a microfermentor. | a medium, pehps, whose main components are extracts of ox liver, and ox and swine pancreas (ehp) was used to develop two alternative methods for axenic cultivation of trophozoites in suspension: with magnetic stirrer and with a 14 l capacity microfermentor, giving the first technic yields of 2.36 x 10(5) to 3.08 x 10(5) and the second in a 14 l capacity microfermentor, which produced 1.65 x 10(5) amebas/ml in one batch and 2.94 to 3.65 x 10(5) of such parasites/ml at semicontinuous flux. | 1992 | 1340322 |
| effects of albendazole on entamoeba histolytica and giardia lamblia trophozoites. | albendazole, a benzimidazole carbamate commonly used for the treatment and control of intestinal helminthic infections, is also useful for the treatment of giardiasis. therefore, it is of interest to determine whether the drug has activity against other intestinal protozoa, such as e. histolytica. the present results demonstrate that albendazole inhibits the growth of e. histolytica trophozoites in axenic cultures and induces fine structural changes such as polyribosome aggregation and loss of c ... | 1992 | 1340323 |
| further studies on the in vitro activity of gossypol as antiamebic agent. | gossypol has an in vitro antiamebic effect, which is 11,39, and 980 times more potent than emetime, metronidazole and diiodohydroxyquinolein, respectively, on five entamoeba histolytica axenic strains. the trophozoite nadp-dependent malic enzyme and alcohol dehydrogenase are inhibited by (+/-)-gossypol, in a noncompetitive manner with respect to the substrate. from the (-)- and (+)- enantiomers, which integrate the natural gossypol mixture, the first one is three and four times more active in in ... | 1992 | 1340324 |
| improved molecular karyotype of entamoeba histolytica. | different electrophoretic conditions were used to improve the molecular karyotype of entamoeba histolytica clone l6 derived from the heterogeneous strain hm1:imss. eleven to 17 bands ranging between 0.3 and over 3 megabases (mb) were resolved by transverse alternating field electrophoresis (tafe). amebic chromosomes presented similar pattern when they were tafe separated at 72, 90 or 120 h running time, but resolution of the bands was increased at 90 h, and the best electrophoretic pattern was o ... | 1992 | 1340325 |
| stability of the zymodemes of entamoeba histolytica in culture. | | 1992 | 1340326 |
| the fine structure of entamoeba histolytica processed by cryo-fixation and cryo-substitution. | recently, the use of cryo-fixation followed by freeze-substitution has been shown to produce a better ultrastructural preservation of cellular components due to the rapidity of the fixation procedure and the lack of distortion during dehydration. when these techniques are applied to study the fine structure of axenically cultured trophozoites of e. histolytica, an improved preservation of cytoplasmic and nuclear components was found. the surface coat of the parasite appears much thicker and unif ... | 1992 | 1340327 |
| entamoeba histolytica: electrophoretic analysis of isolated caps induced by several ligands. | caps induced by several ligands including sera from patients with amebic liver abscess (ala) were isolated by differential centrifugation. purification was verified by scanning and transmission electron microscopy. electrophoretic profiles obtained by sds-page and western blots were compared when probed with the ligand used to induce capping. it was confirmed that sera from different patients recognize different proteins. indeed, electrophoretic profiles reveal that capped membrane proteins vary ... | 1992 | 1340328 |
| variation of proteins and proteinases in entamoeba histolytica lysates containing a protease inhibitor. | sodium dodecyl sulfate (sds)-lysates of e. histolytica trophozoites were analyzed by electrophoresis in simple and gelatin-containing ("substrate") sds-polyacrylamide gels. in simple gels, boiled lysates with para hydroxymercuribenzoate (phmb) had a complex pattern of apparently undegraded proteins; boiled lysates without phmb showed a major 30 kda and four minor (43, 46, 63 and 117 kda) proteins, whereas unheated lysates displayed only the 117 kda protein. using substrate gels no gelatinases we ... | 1992 | 1340329 |
| the effect of axenic versus xenic culture conditions on the total and secreted proteolytic activity of entamoeba histolytica strains. | proteolytic activity was measured in lysates of four axenic and five xenic cultures of different strains of pathogenic e. histolytica, and in five non-pathogenic strains ("e. dispar") growing in xenic culture. there was no significant difference in total proteolytic activity, using either gelatin or albumin as substrate, between the two sets of xenic cultures; the axenic pathogens however had significantly higher levels of activity than the xenic pathogens, the levels appearing to correlate with ... | 1992 | 1340330 |
| phenotypic heterogeneity in the expression of a 30 kda cysteine proteinase in axenic cultures of entamoeba histolytica. | a polyclonal antibody raised against a purified approximately 30 kda cysteine proteinase derived from extracts of axenically grown trophozoites of e. histolytica strain hm-1:imss was used to stain 72 h cultures of the same amebas by indirect immunofluorescence. fluorescence was limited to the outer membrane of the parasite and was either uniformly distributed or more condensed on a segment, at times on a single point of the membrane. in relation to the intensity of fluorescence staining, three d ... | 1992 | 1340332 |
| host tissue destruction by entamoeba histolytica: molecules mediating adhesion, cytolysis, and proteolysis. | entamoeba histolytica, the protozoan parasite causing human amoebiasis, has recently been found to comprise two genetically distinct forms, potentially pathogenic and constitutively nonpathogenic ones. host tissue destruction by pathogenic forms is believed to result from cell functions mediated by a lectin-type adherence receptor, a pore-forming peptide involved in host cell lysis, and abundant expression of cysteine proteinase(s). isolation and molecular cloning of these amoeba products have p ... | 1992 | 1342717 |
| virulence of nonpathogenic zymodemes of entamoeba histolytica isolates from asymptomatic subjects in calcutta, india. | 1. the zymodeme and virulence of 31 entamoeba histolytica isolates obtained from asymptomatic human subjects in calcutta, india are described. 2. virulence was measured by the extent of lesion diameter (mm) induced by each isolate in the liver of golden hamsters and resistance of isolates to non-immune hamster sera in vitro. 3. two nonpathogenic zymodemes, iii (n = 17) and iv (n = 14), were detected among 31 isolates by isoenzyme electrophoresis. most of the zymodeme iii isolates were moderately ... | 1992 | 1342835 |
| seasonal prevalence of protozoan parasites in nsukka, nigeria. | five hundred fresh stool specimens of patients with clinical symptoms of protozoan infections were examined for parasites. during june 1988-may 1991, the total number of positive cases was 261 (52.2 per cent). when the infectivity frequencies were segregated among the samples of different localities, it was observed that 6 (1.2 per cent) persons from the university of nigeria community, 89 (17.8 per cent) urban dwellers and 166 (33.2 per cent) rural populace were infected. the highest incidence ... | 1992 | 1344957 |
| [in vitro decrease of the cytolytic effect of e. histolytica by inhibition of its phosphofructokinase]. | the c14 radioactive label of ppi analogues was incorporated to e. histolytica after 24 hours of incubation at 37 degrees c; more than 90% of trophozoites remained viable. the ppi dependent phosphofructokinase was isolated in order to determine its kinetic parameters. with ppi, the km was 18.06 +/- 0.91 micromol/ml-1. using three different ppi analogues (tetrasodium salts) of (i) 1,1 hydroxy-methyl diphosphonate; (ii) 1,1 hydroxy ethylene diphosphonate; (iii) 1,1 hydroxy-nonano diphosphonate, kii ... | 1992 | 1345117 |
| entamoeba histolytica extrachromosomal circular ribosomal dna: analysis of clonal variation in a hypervariable region. | the ribosomal rna genes of the protozoan parasite entamoeba histolytica are highly repeated and display restriction fragment length polymorphism. using a set of four dna probes spanning the coding region and part of the flanking region of the e. histolytica ribosomal rna genes, an analysis of the dna bands generated by ecori digestion of entamoeba dna is presented. this analysis included five strains of e. histolytica, four strains of e. moshkovskii, and one strain each of e. invadens and e. ter ... | 1992 | 1346767 |
| a model of interaction between entamoeba histolytica and shigella flexneri. | the establishment of a relationship between entamoeba histolytica and certain bacteria may contribute to the expression and/or enhancement of the pathogenicity of this parasite. recent experiments have shown that bacteria expressing mannose-binding lectins on their surface could attach to mannose-containing molecules on the surface of amoebae. in this study, we established a model of interaction between. e. histolytica and shigella flexneri. using well-characterized mutants of s. flexneri, we st ... | 1992 | 1353632 |
| primary sequences of two p-glycoprotein genes of entamoeba histolytica. | two p-glycoprotein genes (ehpgp1 and ehpgp2) from the protozoan parasite entamoeba histolytica were sequenced from a genomic library made with the dna of an emetine-resistant ameba mutant, which overexpresses mrnas homologous to segments of the human mdr1 (p-glycoprotein) gene. the open reading frames for ehpgp1 and ehpgp2 were 1302 and 1310 amino acids long, respectively, and showed a 67% positional identity with each other and 41% and 40% positional identities, respectively, with human mdr1 ge ... | 1992 | 1359408 |
| absence of an association between enteric parasites in the manifestations and pathogenesis of hiv enteropathy in gay men. the gi/hiv study group. | 49 gay men confirmed to be infected with the human immunodeficiency virus (hiv) and 9 hiv seronegative gay men participated in a pilot study comparing clinical status and enteric parasite load with gastrointestinal structure, function and symptomatology. cases included 16/49 (33%) men who were cdc stage ii, 7/49 (14%) who were cdc stage iii, and 26/49 (53%) who were cdc stage iv. the mean cd4-lymphocyte count was 476 +/- 199 (sd)/microliter. the prevalence of enteric parasitic flora was similar ... | 1992 | 1361241 |
| necrotising arteritis in amoebic colitis. | massive intestinal haemorrhage rarely occurs in amoebic colitis. we report a case of caecal amoebic ulcer in a 61 year old diabetic male who presented with massive lower intestinal haemorrhage requiring blood transfusion and emergency surgical intervention. histologically, trophozoites of entamoeba histolytica were seen invading the wall of the submucosal arteries, causing necrotising arteritis. rupture of a necrosed artery probably caused massive haemorrhage. | 1992 | 1363887 |
| differentiation of pathogenic entamoeba histolytica from other intestinal protozoa by riboprinting. | differentiation of the pathogen entamoeba histolytica from the variety of other amebas that can infect the human intestinal tract is vital for accurate diagnosis and treatment. morphology and serology alone are not adequate for positive identification to be achieved. we have developed methods using the polymerase chain reaction to amplify amebal ribosomal rna genes that allow either specific detection of e. histolytica or species identification. | 1992 | 1364099 |
| organization of repeated sequences in the region downstream to rrna genes in the rdna episome of entamoeba histolytica. | the e. histolytica rdna episome consists of a 3.7 kb hindiii fragment located downstream of the rdna inverted repeats. we have determined the complete nucleotide sequence of this fragment and have shown that it is comprised of two families of short tandem repeats, the 170 bp drai repeat and the 144 bp scai repeat. each drai repeat unit consists of 12-mer sequences with near complete homology to yeast consensus autonomously replicating sequence. in addition, a 21-mer subrepeat structure is also p ... | 1992 | 1364100 |
| p-glycoprotein genes of entamoeba histolytica. | six different p-glycoprotein gene segments were identified from an emetine-resistant e. histolytica mutant, which overexpresses mrnas homologous to segments of the human mdr1 (p-glycoprotein) gene. the open reading frames of two completely sequenced genes ehpgp1 and ehpgp2 were 1,302 and 1,310 amino acids long, respectively, and showed a 67% positional identity with each other and 41 and 40% positional identities, respectively, with human mdr1 gene. within each ameba p-glycoprotein were the atp- ... | 1992 | 1364101 |
| pathogenesis in amebiasis: is it genetic or acquired? | | 1992 | 1365521 |
| pathogenic versus nonpathogenic entamoeba histolytica. | | 1992 | 1365522 |
| pathogenesis in amebiasis. | | 1992 | 1365523 |
| pathogenesis in amebiasis. | | 1992 | 1365524 |
| adherence of pathogenic and non-pathogenic entamoeba histolytica strains to neutrophils. | the adherence of polymorphonuclear leukocytes (pmns) to eight pathogenic and nine nonpathogenic strains of entamoeba histolytica was examined. no difference between pathogenic and nonpathogenic strains was found. the addition of different carbohydrates confirmed the importance of the 170-kda lectin of e. histolytica in binding to pmns, corroborated by the finding that treatment of pmns with galactosidase inhibited adherence. inhibition of the microfilament system of e. histolytica using cytochal ... | 1992 | 1372977 |
| inhibition of the complement membrane attack complex by the galactose-specific adhesion of entamoeba histolytica. | the human complement system is an important early host defense against infection. entamoeba histolytica activates the complement system but is resistant to killing by complement c5b-9 complexes deposited on the membrane surface. our aim was to identify components of the amebic plasma membrane that mediate resistance to human complement c5b-9 by screening for neutralizing monoclonal antibodies. a monoclonal antibody was identified that abrogated amebic resistance to c5b-9, and the mab was shown t ... | 1992 | 1381719 |
| channel-forming proteins of man and amoebae. | | 1992 | 1381916 |
| studies in intestinal parasitic disease agents in stools of people in a rural area of nigeria. | a summary of 300 villagers who reported at the parasitology laboratory of the school of medical laboratory technology (s.m.l.t) vom, plateau state, nigeria was carried out for the presence of parasites' cysts, eggs, or larva. of the 300 faecal samples examined using the light microscope after formal-ether centrifugation, 127 (42.3%) harboured one or more parasites. the parasites identified and their prevalent rates were: entamoeba coli (19.0%) necator americanus (17.0%); entamoeba histolytica (4 ... | 1992 | 1390369 |
| case report: amoebic liver abscess complicated by a hepatoduodenal fistula. | amoebic liver abscess (ala) is a common extra-intestinal presentation of amoebiasis caused by entamoeba histolytica. the liver abscess may be complicated by rupture into adjacent structures. common organs involved include thorax, peritoneum and pericardium. rupture into the gastrointestinal tract is extremely rare. we report a patient who developed a hepatoduodenal fistula complicating an amoebic liver abscess. suspicions were raised on finding air in the liver abscess on ultrasound scanning. di ... | 1992 | 1395407 |
| protozoan infections and hiv-1 infection: a review. | reactivation of latent infection is the principal mechanism relating toxoplasma gondii and pneumocystis carinii to hiv. less common is reactivation in leishmania donovani, trypanosoma cruzi, and microsporidian infections. an impaired primary immune response occurs in all these infections, and also with cryptosporidium and isospora belli. association of hiv infection with gut parasites including giardia lamblia and entamoeba histolytica, and also with trichomonas vaginalis infection is likely to ... | 1992 | 1396192 |
| comparative aetiology of childhood diarrhoea in kakamega and kiambu districts, kenya. | two hundred diarrhoea specimens collected during january to february 1988, from rural children aged 0 to 60 months in kakamega district were examined for bacteria, parasites and rotavirus. the results were compared with a sample of 184 diarrhoea specimens matched for month of collection, taken from data collected in the same manner from children in kiambu district. the mean ages of children in the 2 samples did not differ significantly. there were significant differences in the prevalence of spe ... | 1992 | 1396210 |
| primary and secondary structure of the pore-forming peptide of pathogenic entamoeba histolytica. | a pore-forming peptide is implicated in the potent cytolytic activity of pathogenic entamoeba histolytica. using nh2-terminal sequence information of this peptide, the corresponding cdna was isolated. the cdna-deduced amino acid sequence revealed a putative signal peptide and a mature peptide of 77 amino acids including six cysteine residues. computer-aided secondary structure analysis predicted that the peptide would be composed of four adjacent alpha-helices, and cd spectroscopy indicated an a ... | 1992 | 1396552 |
| hookworm infection among the melka sedi banana plantation residents, middle awash valley, ethiopia. | a study of intestinal parasites was done among the people who lived within the banana plantation zone of the melka sedi agricultural enterprise, awash valley, ethiopia in april 1987. the methods of parasite detection were the ritchie formal ether and the charcoal culture method for hookworm larvae species identification. from the total 633 population, 311 were examined at random of whom 60.8% were positive for one or more intestinal parasites. eight parasites were encountered, ascaris lumbricoid ... | 1992 | 1396614 |
| entamoeba histolytica extract and interferon-gamma activation of macrophage-mediated amoebicidal function. | the effect of recombinant murine interferon-gamma (ifn-gamma) and e. histolytica extract (e.h.e.) on macrophage (m phi) activation for amoebicidal activity was examined. peritoneal macrophages were harvested from c57bl/6 and a/j mice and preincubated with ifn-gamma and/or e.h.e. it was found that amoebicidal activity could be induced in both c57bl/6 and a/j-derived macrophages by pretreatment with ifn-gamma and e.h.e. pretreatment of the m phi with e. histolytica extract or ifn-gamma alone did n ... | 1992 | 1398736 |
| kinetics of specific igm in patients of hepatic amoebiasis. | entamoeba histolytica (eh) specific igm was measured in 54 patients with diagnosed amoebic liver abscess (ala), 13 with non-suppurative hepatic amoebiasis (nsha) and 50 controls. the mean levels of eh specific igm, estimated by elisa were significantly raised in patients of invasive amoebiasis (both ala and nsha) compared to controls (p less than 0.05). eh specific igg was also raised in both groups of patients. follow up of patients with ala showed a significant decline (p less than 0.05) in th ... | 1992 | 1398809 |
| determination of taxonomic status of pathogenic and nonpathogenic entamoeba histolytica zymodemes using isoenzyme analysis. | entamoeba histolytica infection results in either asymptomatic colonization or invasion of host tissues leading generally to clinical symptoms. zymodemes studies have demonstrated a correlation between isoenzyme profiles and clinical presentation. thus, strains have been attributed to pathogenic or nonpathogenic groups according to their zymodeme. to determine the taxonomic relationship of these two groups, the isoenzyme profiles of 14 loci of 38 e. histolytica strains (pathogenic and nonpathoge ... | 1992 | 1403982 |
| [distinguishing between pathogenic and nonpathogenic isolates of entamoeba histolytica by polymerase chain reaction]. | | 1992 | 1404938 |
| adherence between entamoeba histolytica trophozoites and undifferentiated or dmso-induced hl-60 cells. | differentiation of promyelocytic hl-60 cells along the pathway toward granulocytes using dimethyl-sulfoxide (dmso) led to increased expression of the adhesion molecules cd11b (mac-1), cd11c (p150,95), and cd35 (cr1). undifferentiated and differentiated hl-60 cells adhered similarly to entamoeba histolytica trophozoites, and monoclonal antibodies against adhesion molecules cd11b and cd11c did not inhibit adherence. we therefore suggest that cd11b and cd11c are not involved in the adherence betwee ... | 1992 | 1409534 |