| [intestinal amebiasis]. | infection produced by entamoeba histolytica constitutes a clinical problem of great importance due to its frequence and serious complications. age, diagnosis and clinical manifestations, phisic exploration, lab. procedures, proctosigmoidoscopy, radiology, complications, pathologic anatomy, statistical reports from the ministry of health and social asistence in addition we emphasize on the importance of an accurate and precocious diagnosis. we expose an effective terapeutic regimen for the differ ... | 1975 | 816701 |
| [the laboratory diagnosis of amoebic infections in man (author's transl)]. | the rapid progress made in both theoretical and practical fields in laboratory medicine, in general, and in microbiological diagnostic procedures, in particular, have also led to a significant enlargement of, and improvement in laboratory diagnosis of amoebic infections in man. in all intestinal infections with entamoeba histolytica the demonstration of the parasite is absolutely essential, but is facilitated nowadays by the great improvement in technical conditions. immunodiagnostic methods hav ... | 1977 | 842029 |
| [toxoplasmosis. anatomo clinical characteristics and morphological indentification of the parasite using the silver-impregnation technic]. | the clinical and anatomicopathological characteristics of acute and chronic toxoplasmosis are described as they affect different structures and systems. the lesion to adrenal glands is compared to that seen in disseminated herpes simplex; however, the question remains as to why in both congenital infections, necrosis of adrenal glands appears without inflammatory reaction. the investigation of special techniques for localization and identification of toxoplasma gondii "groups" or cysts, leads to ... | 1977 | 843413 |
| plasmalemmal modifications of entamoeba histolytica in vivo. | we used electron microscopy to study entamoeba histolytica trophyozoites in rectal and liver lesions. the amebal surface had bleblike structures. also noted were subplasmalemmal vacuoles and plasmalemmal extensions generally similar to the "surface lysosome" and "trigger" previously described. we found on serial sections that the plasmalemmal extensions were dendritic complexes enfolding the membrane of the subplasmalemmal vacuole and extending to contact host tissue. we postulate that membrane- ... | 1977 | 845740 |
| the pattern of parasitic infection in human gut at the specialist hospital, benin city, nigeria. | in a survey of 6213 persons conducted between january 1973 to december 1974, at the specialist hospital, benin city, the most common helminths were necator americanus (16.8%), ascaris lumbricoides (19l5%) and trichuris trichiur (5-9%). dicrocoelium hospes (0-06%) was also recorded and this may become an important liver parasite of man in nigeria. its snail vectors are believed to be species of limicolaria and achatina which are widely dispersed in nigeria. entamoeba coli and e. histolytica showe ... | 1977 | 849017 |
| filamentous virus-like material in the nucleus of entamoeba coli from the human colon and from culture. | | 1977 | 888506 |
| endemic amebiasis in an extended family. | two cousins from a large spanish-american family were simultaneously diagnosed as having amebic liver abscesses. survey of 183 extended-family members revealed that 45.7% of 162 had a positive amebiasis indirect hemagglutination test and 12.6% of 111 had cysts or trophozoites of entamoeba histolytica demonstrated in a single stool examination. a total of five family members had had liver abscesses; two deaths had occurred. in a random sample survey of the remainder of the community, only one per ... | 1977 | 889003 |
| cellular immune responses to amoebic liver abcess in the guinea-pig. | guinea-pigs infected in the liver with the biswas strain of entamoeba histolytica showed no dermal hypersensitivity but showed positive lymphocyte transformation and macrophage-migration inhibition. the time sequence showed an activated response at 4 days after infection, a full response at 8 days when the liver abscesses were resolving and a waning response at 12 days when the abscesses had healed. | 1977 | 891028 |
| [traveler's diarrhea]. | travellers' diarrhea afflicts up to 50 percent of tourists in certain geographic regions. it is a syndrome caused by a variety of pathogens, the most common of which is enterotoxigenic e. coli. other responsible pathogens include salmonellae, shigellae, entamoeba histolytica, yersinia enterocolitica, giardia lamblia and virus-like agents, seldom vibrios. symptoms persist for only a few days and are only serious in 1/5 of the cases. | 1977 | 914154 |
| [disinfecting effect of ammonia water on the cysts of intestinal protozoa in the feces]. | | 1977 | 927349 |
| protozoan and helminthic infections of the intestines of humans in the inyanga area of rhodesia. | a survey of the protozoan and helminth species which infest the intestines of the people in the lnyanga area of rhodesia has revealed a wide range of species. of the protozoa, entamoeba coli was by far the commonest species encountered (45,8%) and of the helminths, hookworms (8,2%) were the most common. | 1976 | 945617 |
| parasitology survey in northern sumatra, indonesia. | a parasitology survey was conducted in five villages in north sumatra, indonesia. a total of 3,207 blood smears, 2,066 stool specimens and 969 sera were examined. sixty (1.9%) inhabitants had malaria (plasmodium vivax 41, p. falciparum 19), and 20 had brugia malayi microfilaraemia. the most common intestinal helminths were trichuris trichiura (87%), ascaris lumbricoides (75%) and hookworm (58%). other helminths found in low numbers were enterobius vermicularis, strongyloides stercoralis, taenia ... | 1976 | 950682 |
| tinidazole: a review of its antiprotozoal activity and therapeutic efficacy. | tinidazole, a synthetic imidazole derivative, has been used in the oral treatment of several protozoal infections - trichomoniasis, giardiasis and amoebiasis. among the protozoal organisms inhibited by tinidazole are trichomonas vaginalis, trichomonas foetus, and entamoeba histolytica. in vitro, tinidazole has been shown to possess antiprotozoal activity at least comparable to, and in some cases greater than, metronidazole. tinidazole also has activity against some gram-negative anaerobic bacill ... | 1976 | 954609 |
| therapeutic evaluation of oxantel pamoate (1, 4, 5, 6-tetrahydro-1-methyl-2-[trans-3-hydroxystyryl] pyrimidine pamoate) in severe trichuris trichiura infection. | the efficacy of oxantel pamoate (1, 4, 5, 6-tetrahydro-2-[trans-3-hydroxystyryl]-1-methyl pyrimidine pamoate) was evaluated in 25 children with severe trichuris infection. the presence of chronic dysentery and sigmoidoscopic demonstration of whipworms in the intestinal mucosa were the criteria for inclusion in the trial. most of these patients had severe anemia, rectal prolapse, digital clubbing, hypoproteinemia, and growth retardation. there were a high incidence of concomitant parasitism with ... | 1976 | 961973 |
| the occurrence of human intestinal parasites in finland. | a survey of the occurrence of intestinal parasites covered 243 inmates of institution for the mentally retarded, 537 hospital patients from 4 areas, 100 military servicemen, and 65 prisoners. two faecal samples from each patient were examined by the formalin-ether concentration technique. among the mentally retarded the prevalence of intestinal parasites was high, 41.3% among children and 51.5% among adults. the commonest parasite was entamoeba coli. in helsinki, the prevalence among children in ... | 1976 | 968457 |
| a source of error in the laboratory diagnosis of amoebiasis. | | 1976 | 977966 |
| observations on the intestinal protozoa infecting man in rhodesia. | humans in rhodesia harbour a wide range of intestinal protozoa. of the species included, entamoeba histolytica, entamoeba coli and giardia lamblia have previously been recorded. other species which are either rarely reported or which have previously never been reported from this country, include trichomonas hominis, chilomastix mesnili, enteromonas hominis, retortamonas intestinalis, balantidum coli,entamoeba hartmanni,entamoeba histolytica laredo. endolimax nana, dientamoeba fragilis and isospo ... | 1976 | 988643 |
| action of antiamoebic drugs on ribonuclease and deoxyribonuclease activities of the caeca of rats infected with a virulent strain entamoeba histolytica. | | 1976 | 992730 |
| heavy trichuris infection and amoebic dysentery in orang asli children. a comparison of the two diseases. | children with heavy trichuris infestation were compared with paediatric amoebic dysentery patients and normal children. heavy trichuris infestation was diagnosed by visualization of worms on anoscopy. patients with heavy trichuris infection had a longer duration of disease, more frequent hospitalization and a higher rate of rectal prolapse than did patients with amoebiasis. five trichuris children also had clubbing. trichuris patients had lower mean haematrocrits (27%) and serum albumin (3-3 gm% ... | 1976 | 1006759 |
| first isolation of entamoeba moshkovskii from sewage in australia. | | 1976 | 1006774 |
| [oral protozoan diseases: experimental possibilities[]. | | 1976 | 1020710 |
| parasitological survey in gorontalo north sulawesi, indonesia. | a survey for blood and intestinal parasites was carried out in the municipality of gorontalo on the minahasa peninsula of north sulawesi. a total of 156 stool specimens were obtained from 83 males and 73 females. trichuris trichiura, ascaris lumbricoides, hookworm, unidentified dicrocoeliid-like ova and entamoeba coli, in that order, were the most common intestinal parasites detected. other intestinal parasites found less frequently were entamoeba histolytica, iodamoeba bütschlii, endolimax nan ... | 1976 | 1030844 |
| intestinal and blood parasites of man in bireuen and takengon, aceh province, sumatra, indonesia. | a survey for blood and intestinal parasites was carried out in aceh province of north sumatra, indonesia. a total of 348 stool specimens were obtained from 167 males and 181 females ranging in age from 6 months to 70 years. over 98% of the population sampled were found infected with at least one intestinal parasite. ascaris lumbricoides, trichuris trichiura, hookworm and entamoeba coli, in that order, were the most common parasites detected. other intestinal parasites found less frequently were ... | 1976 | 1030850 |
| amebic colitis mistaken for inflammatory bowel disease. | in ten patients, amebic colitis was mistakenly diagnosed as ulcerative colitis or crohn disease of the colon because of the similarity of history, physical examination, and routine laboratory studies as well as findings on proctoscopic and barium enema examination. multiple stool examinations failed to demonstrate ova or trophozoites of entamoeba histolytica. routine examinations of stools for ova and parasites are inadequate and even a meticulous search for amebas in fresh stool, in scrapings f ... | 1975 | 1052663 |
| entamoeba histolytica in new zealand. | | 1976 | 1067494 |
| [entamoeba gingivalis--factor in periodontal diseases]. | | 1976 | 1077247 |
| [entamoeba gingivalis incidence in normal gingiva, catarrhal and experimental gingivitis in man]. | | 1976 | 1077250 |
| uptake of metronidazole and its effect on viability in trichomonads and entamoeba invadens under anaerobic and aerobic conditions. | [(14)c]metronidazole used at the chemotherapeutic concentration of 10 mug/ml is taken up rapidly by the anaerobic protozoa tritrichomonas foetus, trichomonas vaginalis, and entamoeba invadens kept under anaerobic conditions. it can be calculated that within 30 to 60 min the intracellular concentration of the label is 50 to 100 times higher than in the medium. the presence of air markedly suppresses the uptake in the trichomonads and abolishes it in e. invadens. the suppression disappears after a ... | 1976 | 1083712 |
| massive development of amebas in the large intestine. fedor aleksandrovich lesh (lösch). | fedor aleksandrovich lesh (lösch) 1840-1903. although lösch was a distinguished russian physician, little has been written about him in english. a graduate of the medico-chirurgical academy in st. petersburg (1863), he defended a doctoral thesis on pulmonary embolism and infarction in 1866, and became an assistant professor of medicine in 1871. from 1872 to 1885 he was a lecturer on diagnosis at the women's medical college and a consultant to the nikolayev military hospital. in 1885 he became pr ... | 1975 | 1098489 |
| surveillance of some infectious diseases among aircrew personnel in southeast asia. | a 2-year analysis of specimens among aircrew personnel in southeast asia is reported. stool specimens were examined for intestinal parasites and enteric bacteria, blood smears for blood parasites, and sera tested for transaminases (sgot) and antibodies to entamoeba histolytica, toxoplasma gondii, chikungunya and japanese encephalitis viruses, rickettsia tsutsugamushi, leptospira sp. and pseudomonas pseudomallei. one to four specimens each were obtained from 537 adult males and 20 females. there ... | 1975 | 1100040 |
| eimeria taterae sp. n. and other intestinal parasites from the antelope rat, tatera indica in baghdad district. | of 8 fecal specimens from the antelope rat, tatera indica hardwicke examined, 4 were found positive for intestinal parasites. among these was eimeria taterae sp. n., sporulated oocysts of which are described, their characteristics serving for the diagnosis of this species. entamoeba coli cysts, as well as ova of hymenolepis sp. and trichuris sp., were also found. | 1975 | 1117434 |
| [extensiveness of occurrence of protozoa in man's oral cavity]. | | 1975 | 1121870 |
| dogs and intestinal parasites: a public health problem. | the stools of 239 stray dogs were examined for intestinal parasites. of the helminths found, toxocara canis (43.5%), tapeworms (25.5%), ascaris species (21.3%) and hookworms (12.5%) were the commonest. of the protozoans found, isospora species and entamoeba coli were the most prevalent. an unusual feature of the present study was the finding of ascaris species. the importance of the high prevalence of intestinal parasites in dogs, the close contact of humans with dogs' excreta and the possible r ... | 1975 | 1125888 |
| the mechanism of formation of inhibitor-induced ribosome helices in entamoeba invadens. | helices andaggregates of helices (chromatoid bodies) composed of ribosomelike particles appear in cysts and slow-growing trophozoites of entamoeba invadens. we found that similar helix aggregates were formed abundantly in actively growing e. invadens trophozoites treated with a variety of direct or indirect inhibitors of protein synthesis. the inhibitor-induced helices appeared cytochemically and ultrastructurally identical to those seen in cysts. numerous single helices and small arrays occurre ... | 1975 | 1133115 |
| comparision of the structure and function of polysomal and helical ribosomes from entamoeba invadens. | some structural and functional properties of ribosomes from polysomes and from helix aggregates of entamoeba invadens have been compared by sucrose gradient analysis and assays of in vitro protein synthesis. actively growing trophozoites, lacking helices, presented normal polysome profiles in sucrose gradients. the single large ribosomal helix aggregate (chromoatoid body) of cysts diappeared as the cells were disrupted. gradient profiles of cyst extracts contained predominantly large and small r ... | 1975 | 1133116 |
| epizootic of fatal amebiasis among exhibited snakes: epidemiologic, pathologic, and chemotherapeutic considerations. | an epizootic of reptilian amebiasis seems to have caused the death of 15 to 16 large and valuable captive snakes (boas, pythons, and anacondas) occupying one of 5 large display dioramas in the steinhart aquarium of the california academy of science, golden gate park, san francisco. subsequent review of previous snake deaths in the colony indicated that of 464 snakes that had died since early 1969, 89 snakes had intestinal or hepatic lesions, and 80 of these snakes had pathologic features which i ... | 1975 | 1147335 |
| membrane structure and surface coat of entamoeba histolytica. topochemistry and dynamics of the cell surface: cap formation and microexudate. | treatment of living entamoeba histolytica cells with low concentrations of concanavalin a (con a) and peroxidase results in redistribution of the plasma membrane con a receptors to one pole of the cell where a morphologically distinct region--the uroid--is formed. capping of con a receptors is not accompanied by parallel accumulation of ruthenium red-stainable components. in capped cells, the pattern of distribution of acidic sites ionized at ph 1.8 (labeled by colloidal iron) at the outer surfa ... | 1975 | 1150744 |
| phospholipids of entamoeba invadens. | | 1975 | 1190798 |
| prevalence of intestinal parasites in latino residents of chicago. | in 1970, 250,000 spanish-speaking persons resided in chicago, 80,000 of whom were puerto rican. because migration to chicago is constantly occurring from areas where intestinal parasites are endemic, a survey was conducted in a predominantly puerto rican neighborhood to determine the prevalence of these parasites and to provide impetus for further case finding and treatment programs. the survey was part of an epidemiologic and cutreach workers going door-to-door obtained histories, hematocrits, ... | 1975 | 1202954 |
| the surface and cytoplasmic membranes of entamoeba invadens (rodhain 1934)--ii. polypeptide and phospholipid composition. | | 1975 | 1204332 |
| spontaneous lesions and parasites of the mongolian gerbil, meriones unguiculatus. | four-hundred-eighty mongolian gerbils, meriones unguiculatus [uclp:(mon)], most of which were experimentally infected with filarial worms, were examined for spontaneous lesions. previously unrecognized lesions included cutaneous squamous cell carcinoma, duodenal adenocarcinoma, malignant blue nevus, hepatic choleangiocarcinoma, malignant hemangiopericytoma of the uterus, ovarian teratoma, chronic interstitial nephritis, renal cortical retention cysts, splenic hemangiomas, and various histologic ... | 1975 | 1207042 |
| intestinal parasites in university of dacca students. | nine hundred and thirty three students of dacca university were examined: mean haemoglobin levels were 80.5 per cent for men and 70.0 per cent for women. five hundred and thirty three (57.3%) of the students had single or multiple intestinal parasitic infections, of which ascaris lumbricoides (366), entamoeba histolytica (113) and trichuris trichiura (99) were the commonest. it is suggested that chronic nutritional deficiencies are compounded by these parasites, leading to low weights, and low h ... | 1975 | 1214308 |
| [evaluation of helminth carrier rates among foreign workers (author's transl)]. | the evaluated helminth carrier rate among foreign workers give rise to many problems under socio-economic, hygienic and occupational aspects. the evaluation of helminth infestation is connected with considerable uncertainty. based on the author's examinations these problems are critically investigated from the point of view of both, the employer and the employee. it is shown in tables that in the institute of hygiene and microbiology of würzburg during 1973/1974 497/702 respectively faecal exami ... | 1975 | 1214697 |
| a case of cutaneous amoebiasis. | the present report concerns a patient with ulceration around an artificial anus. the condition had been treated for some time as pyoderma gangrenosum but finally proved to be cutaneous amoebiasis; the presence of entamoeba histolytica was demonstrated. a cure was achieved with metronidazol (flagy) and diloxanide furoate (furamide) given orally. | 1975 | 1225662 |
| [chemotherapeutic effects of nitro compounds. 1. nitroanilines]. | more than 200 nitro compounds, most of them nitroaniline derivatives substituted with one or more radicals having a basic reaction, were prepared and investigated as to their therapeutic activity against bacteria, fungi, protozoa, helminths, viruses and tumors. several mono-nitrobenzenes with a radical having a basic reaction showed weak in-vitro activity against gram-positive bacteria and against crocker's sarcoma 180; they also showed systemic activity against nematodes (aspiculuris tetraptera ... | 1975 | 1242309 |
| treatment of intestinal parasites in australian aboriginal children. | five to nine-year-old aboriginal children on mitchell river community maintained a satisfactory growth rate in the presence of multiple infections with intestinal parasites. intensive treatment was successful in eliminating giardia lamblia, entamoeba histolytica, and strongyloides stercoralis infections, but reinfection with g. lamblia was rapid. treatment failed to produce any growth spurt in the group. this may have been due to the rapidity of reinfection, but raises the question of whether in ... | 1976 | 1263912 |
| intestinal parasitic infestation among parturients in trinidad and tobago. | 1. in this limited survey, 42.5% of parturients were found to be infested with intestinal parasites. 2. hookworm, trichuris and entamoeba infestations were the most common. 3. race, social class and locality influenced in the incidence of the different types of infestations. 4. those infested with hookworm had longer labor, smaller babies and higher incidence of prematurity. | 1976 | 1270222 |
| recognition of entamoeba histolytica lipophosphoglycan by a strain-specific monoclonal antibody and human immune sera. | western blot analysis showed that the monoclonal antibody 2d7.10 recognized lipophosphoglycan (lpg) from entamoeba histolytica hm-1:imss. sodium dodecyl sulfate-polyacrylamide gel electrophoresis (sds-page) pattern of [3h]galactose-labeled lpg and western blot analysis of total lysate of e. histolytica with 2d7.10 revealed patterns similar to that of lpg with 2d7.10. this antibody could also immunoprecipitate purified lpg from the strain hm-1:imss after biosynthetically labeling with [3h]galacto ... | 1992 | 1283004 |
| susceptibility of an emetine-resistant mutant of entamoeba histolytica to multiple drugs and to channel blockers. | previously a cloned emetine-resistant mutant of the protozoal parasite entamoeba histolytica was shown to overexpress a gene for an ameba homolog of the mammalian p-glycoprotein, a plasma membrane pump that removes hydrophobic drugs from multidrug-resistant tumor cells. three sets of experiments were performed to better characterize the multidrug-resistant phenotype of the emetine-resistant amebae. first, the emetine resistance of the mutant amebae was reversed by concentrations of calcium and s ... | 1992 | 1283295 |
| membrane perforation by entamoeba histolytica: structural implications derived from the sequence of the pore-forming peptide. | the pore-forming peptide amebapore is considered crucial for the cytolytic activity of e. histolytica. isolation and subsequent molecular cloning of the peptide allowed predictions as to its secondary structure, most of which were confirmed by experimental data. here, a computer-aided approach is applied to identify, within the peptide, highly amphipathic helical segments predicted to interact with biological membranes. two putative alpha-helices fulfill the criteria of membrane-seeking domains ... | 1992 | 1285083 |
| cloning and partial characterization of an antigen detected on membrane surfaces of non-pathogenic strains of entamoeba histolytica. | a novel and unique mab (318-28) which specifically interacts with a 60 kda antigen that is found only on the surfaces of non-pathogenic (np) strains of e. histolytica has been recently characterized. the antigen appears to be present also on (np) cyst forms of amebas but was not detected on any of the various (p)-strains tested. it was also not found on other entamoeba species such as moshkovskii, laredo, huff, coli, gingivalis, or invadens. clinical trails for the differentiation of (p) and (np ... | 1992 | 1285084 |
| sequence and structure of the galactose adherence lectin of entamoeba histolytica. | | 1992 | 1285085 |
| a rapid colorimetric assay with the tetrazolium salt mtt and phenazine methosulfate (pms) for viability of entamoeba histolytica. | a rapid colorimetric assay for viability of e. histolytica trophozoites using the tetrazolium salt mtt and phenazine methosulfate (pms) was developed. the mtt is normally reduced by dehydrogenases of viable cells and transformed to formazan, a colored compound; the reaction is catalyzed by pms. trophozoites of e. histolytica were incubated in the presence of mtt and pms during 15, 30, 45, 60 and 75 min, at 37 degrees c. trophozoites killed by heat or cold were used as controls. the formazan prod ... | 1992 | 1285086 |
| structural organization of chromatin during the cell cycle of entamoeba histolytica trophozoites. | the nuclear division of e. histolytica trophozoites was analyzed by using specific stains for dna, with the aim to define the sequential changes of chromatin during its life cycle. furthermore, we characterized the internal structural arrangements of microtubules in the microtubular organizing center (mtoc) and determined the number of chromosomes and its association with the spindle. the mtoc is formed by multiple microtubule-nucleating centers, that are involved in the displacement of dna duri ... | 1992 | 1285087 |
| human infections of entamoeba chattoni masquerade as entamoeba histolytica. | | 1992 | 1287923 |
| entamoeba polecki: human infections in venezuela. | | 1992 | 1287924 |
| energy metabolism of ancestral eukaryotes: a hypothesis based on the biochemistry of amitochondriate parasitic protists. | parasitic amitochondriate protists, representatives of early branches of eukaryote evolution, differ considerably in their central, energy metabolism from mitochondrion-bearing cells. these differences are: significant metabolic functions of inorganic pyrophosphate, major role of iron-sulfur proteins in key metabolic steps and in hydrogenosome-bearing organisms the disposal of electrons by h2 formation. cytochrome-mediated electron transport and electron transport-linked phosphorylation are abse ... | 1992 | 1292665 |
| pilot studies on the occurrence of some infectious diseases in two different areas in south yemen (aden). part i. parasitology. | two pilot studies on malaria, leishmaniasis, schistosomiasis and intestinal parasites were carried out in 104 children 6-15 years old from villages in the lowland and highland areas of south yemen in november 1988. some of the results presented are in the order lowland and highland. the occurrence of malaria parasites in blood smears was 6.7% and 3.8%. p. falciparum was detected only. the antimalarial ifat antibodies were proved in 66.7% and 11.5% respectively. ifat antibodies against leishmania ... | 1992 | 1293209 |
| isolation of entamoeba histolytica from arthritic knee joint. | amoebae were isolated from an arthritic knee of a male patient. the organisms grew in egg yolk infusion medium, and in diamond's biosate iron-serum-33 medium, which contained trypanosoma cruzi, and were identified microscopically as entamoeba histolytica. furthermore, amoebae-like organisms in aspirate and cultures were immunocytochemically identified using monoclonal antibodies against e. histolytica. on the basis of the morphologic and immunologic observations, it was concluded that the knee l ... | 1992 | 1295146 |
| [the living and health conditions of palestinian children]. | young people constitute 46% of the population in the west bank of israel and 48% in the gaza strip with a rate of growth of 3.1% and 3.4%, respectively. their living conditions are characterized by lack of sanitation, shortage of water (especially in comparison to israel proper), unemployment, curfews and closing of schools during tense periods by the military authorities as collective punishment for incidents. since 1967, israel has controlled and administered the health care service in these ... | 1992 | 1296273 |
| inhibition of target cell adhesion by monoclonal antibody to the 66 kd surface antigen of entamoeba histolytica. | the adherence of entamoeba histolytica trophozoites to target cells was studied using a monoclonal antibody to a major surface antigen of 66 kd. preincubation of trophozoites with monoclonal antibody decreased their ability to adhere to and engulf erythrocytes and to destroy chinese hamster ovary cells. the monoclonal antibody was specific for the 66 kd antigen, which is possibly a major participant in the adhesion that precedes phagocytosis and cytopathic effects. | 1992 | 1296611 |
| partial purification of acid phosphomonoesterase in axenically grown entamoeba histolytica nih-200. | entamoeba histolytica possesses significant acid phosphatase activity as compared to alkaline phosphatase activity. the acid phosphatase activity in the amoebic cells eluted at higher saline concentration as three distinct peaks at 200, 300 and 400 mm sodium chloride. | 1992 | 1296952 |
| colonic immunity in patients and amoebic liver abscess. | secretory immunoglobulin a (s-iga), coproantibody titre (antiamoebic) and iga, igg, igm immunocytes in rectal mucosa were studied in 13 patients with amoebic liver abscess (ala) prior to and 4-6 weeks after completion of antiamoebic therapy. ten asymptomatic entamoeba histolytica cyst passers and 17 healthy age and sex matched volunteers served as controls. fecal s-iga levels and counts of iga bearing immunocytes in mucosa were significantly higher in patients with ala and cyst passers as compar ... | 1992 | 1296954 |
| effect of hamster liver passage on the isoenzyme patterns of entamoeba histolytica. | the effect of hamster liver passage on the isoenzyme patterns of isolates of entamoeba histolytica was investigated. three isolates, f, g and t were originally obtained from patients with acute amebic dysentery and another strain, c, was obtained from an asymptomatic carrier. all these strains were maintained for over two years in axenic culture. the isoenzyme pattern (zymodeme) of hexokinase (hk), phosphoglucomutase (pgm) and glucose phosphoisomerase (gpi) of these strains was found to belong t ... | 1992 | 1304461 |
| amebiasis: a problem solved. what now? bernardo sepúlveda memorial lecture. | | 1992 | 1308698 |
| local and systemic antibody response in balb/c mice immunized with entamoeba histolytica trophozoites. | several immunization schedules with e. histolytica trophozoites were tested on balb/c mice in order to induce antibody responses, both in intestinal secretions and in serum. mice were immunized either orally, systemically, or using one of two combined schedules: the oral route followed by the systemic route (footpad), or vice versa. each of the immunization schedules used in this project induced an anti-e. histolytica antibody response and there appears to be a correlation between the immunizati ... | 1992 | 1308795 |
| chemotherapy and immunity in opportunistic parasitic infections in aids. | parasitic diseases are endemic in parts of the tropics, but there is no convincing evidence that their prevalence or incidence is increasing due to the hiv epidemic. available scientific data on parasitic infections in patients with the acquired immunodeficiency syndrome (aids) suggests a predominance of pneumocystis carinii, toxoplasma gondii and cryptosporidium spp. for reasons which are unclear, parasitic infections such as plasmodium falciparum, strongyloides stercoralis and entamoeba histol ... | 1992 | 1308934 |
| immunochemical characterisation of a 29-kda surface-associated molecule of entamoeba histolytica and its recognition by serum from patients with amoebiasis. | a 29-kda cytotoxic molecule of axenically-grown pathogenic entamoeba histolytica (strain hm1) was purified from an amoebic extract by immuno-affinity chromatography with monoclonal antibodies. immunoreactivity of the purified 29-kda molecule altered significantly (p less than 0.01) after exposure to heat or trypsin, but remained unaltered after treatment with sodium metaperiodate. the 29-kda molecule was recognised by serum from each of 13 patients with amoebic liver abscess. in an elisa system, ... | 1992 | 1309884 |
| macrophage cytotoxicity against entamoeba histolytica trophozoites is mediated by nitric oxide from l-arginine. | the killing of entamoeba histolytica trophozoites by phagocytes involves oxidative and nonoxidative mediators. in this study, we determine whether l-arginine-derived nitric oxide (no) is involved in the killing of e. histolytica trophozoites by activated murine macrophages in vitro. elicited peritoneal and bone marrow-derived macrophages activated with ifn-gamma alone or with ifn-gamma and lps killed 62 to 73% of amebae, concomitant with increased levels of nitrate (no2). depletion of l-arginine ... | 1992 | 1318338 |
| ubiquitin of entamoeba histolytica deviates in six amino acid residues from the consensus of all other known ubiquitins. | the amino acid sequence of ubiquitin from entamoeba histolytica, as deduced from a cdna nucleotide sequence, deviated at six positions from the consensus of all other known ubiquitins (ranging from trypanosoma cruzi to homo sapiens). the corresponding residues were scattered over the primary sequence, but came close together on the surface of the folded protein structure. we conclude that (i) e. histolytica branched off very early from the main eukaryotic line, and (ii) this organism may yield c ... | 1992 | 1322840 |
| population genetics of microbial organisms. | population data suggest that many parasitic protozoa (e.g. trypanosoma, leishmania, entamoeba and giardia) reproduce clonally, but this hypothesis has been highly controversial for plasmodium falciparum. although reproduction is predominantly clonal in the enteric bacteria escherichia coli and salmonella, the level of recombination affecting short (< 1 kb) regions of the chromosome is sufficient such that many genes are obviously mosaics of different ancestries. transposable insertion sequences ... | 1992 | 1335810 |
| [electron microscopic enzyme cytochemistry of entamoeba histolytica trophozoite]. | electron microscopic enzyme cytochemical reactions of entamoeba histolytica trophozoite showed that acid phosphatase (acp) and cytidine monophosphatase (cmpase) were located in the lysosomes. the lysosome containing enzymes were distributed in the endoplasm and beneath the plasmalemma, and the releasing enzymes by lysosomes excreted outside of the plasmalemma and caused the injury to host cells. the cytochemical positive reactions of catalase and glucose-6-phosphatase (g-6-pase) showed that e. h ... | 1992 | 1337724 |
| recent progress in the molecular biology of entamoeba histolytica. | entamoeba histolytica, a protozoan parasite causing human amoebiasis, has recently been found to comprise two genetically distinct forms, potentially pathogenic and constitutively nonpathogenic ones. host tissue destruction by pathogenic forms is believed to result from cell functions mediated by a lectin-type adherence receptor, a pore-forming peptide involved in host cell lysis, and abundant expression of cysteine proteinases. comparisons of these products from pathogenic and nonpathogenic e. ... | 1992 | 1338130 |
| entamoeba histolytica: phylogenetic considerations. a review. | comparison of 16s rrna sequences alone positions e. histolytica in rrna-based phylogenies branching after flagellates such as euglenoids and kinetoplastids, whereas morphological and functional features had suggested an earlier branching and different relationships. as several characteristics of this parasite do not precisely adjust to the phylogenetic frame obtained by comparison of ribosomal sequences, and the inferences from this approach could be skewed because of the high a+t content of ent ... | 1992 | 1340267 |
| subcellular distribution of amebapain, the major cysteine proteinase of entamoeba histolytica. | amebapain, the major cysteine proteinase of e. histolytica, appears to be important both for digestion and as a cytotoxic factor. by immunocytology we established that amebapain was bound to matrix-like structures both on the cell surface and within subcellular vesicles; the amebapain co-localized with pinocytized iron oxide, suggesting that the enzyme recycles between plasma membrane and pinocytic vesicles. we conclude from these data that (i) the pinocytic vesicles in e. histolytica perform fu ... | 1992 | 1340268 |
| entamoeba histolytica collagen binding proteins. | three main collagen binding proteins from trophozoites of e. histolytica have been isolated: 105 kda, 56 kda and 30 kda. they display a type i collagenolytic activity. the enzyme behaves as a mammalian collagenase regarding to its activity, collagen degradation products and inhibitors. antibodies against affinity-purified molecules inhibit the binding of trophozoites to collagen substrates. indirect evidence suggests that the 30 kda molecule is the putative collagen receptor. these surface molec ... | 1992 | 1340269 |
| high resolution two-dimensional protein gel evidence of differential gene expression during entamoeba encystation. | as an initial step of investigation into the regulatory mechanisms of encystation in entamoeba, we compared the protein profiles of newly formed cysts and trophozoites of e. invadens using high resolution two-dimensional page and digitized video image analysis of silver stained gels. a total of 155 proteins unique to trophozoites and a total of 72 proteins unique to cysts were detected. five of the most prominent cyst specific proteins were identified as candidates for further study. these resul ... | 1992 | 1340270 |
| collagenase activity in clinical isolates of entamoeba histolytica maintained in xenic cultures. | several isolates of pathogenic and non-pathogenic e. histolytica from xenic cultures were tested for their capacity to digest native type i collagen gels. the results demonstrate that the pathogenic isolate hm48:imss has a high collagenolytic activity. the non-pathogenic isolates, hm43:imss, hm44:imss and hm46:imss showed significantly lower collagenolytic activity. six groups showing different degrees of collagenase activity or non-activity were separated from the non-pathogenic isolates by a p ... | 1992 | 1340271 |
| identification and partial purification of an entamoeba histolytica membrane protein that binds fibronectin. | a 37 kda protein has been described as a putative receptor for fibronectin (fn) on e. histolytica trophozoites (1). we have now identified a membrane protein that binds biotinylated fibronectin (bfn) with an apparent molecular weight of 140 kda. using bfn we were able to follow this protein during partial purification through deae-cellulose and fn-sepharose chromatography. antisera prepared against a peptide corresponding to the deduced amino acid sequence for the putative receptor binding site ... | 1992 | 1340272 |
| fibronectin "receptor" in entamoeba histolytica: purification and association with the cytoskeleton. | preferential binding of e. histolytica trophozoites to fibronectin (fn) is mediated by a 37 kda peptide (1). we report now the further purification of this "putative" fn receptor (fn-r) and its characterization as an external membrane component also associated to the cortical cytoskeleton. the fn-r was solubilized from triton-cytoskeletons and separated by chromatography in fn-sepharose. the fn-r, labeled when live trophozoites were iodinated with 125i, remained associated to the cytoskeleton to ... | 1992 | 1340273 |
| actin associated proteins of entamoeba histolytica. | treatment of e. histolytica hm1-imss trophozoite extracts to conditions that produce gels of actin and associated cytoskeletal proteins in other ameboid cells caused formation of macroscopic actin rich complexes (arcs). the one-dimensional page protein profile of this arc was similar to those of dictyostelium and acanthamoeba actin gels. formation of the e. histolytica arcs was enhanced by added lipids. in addition to actin, the arc was enriched with proteins that showed cross-reactivity to anti ... | 1992 | 1340274 |
| inhibition of the complement membrane attack complex by the galactose-specific adhesin of entamoeba histolytica. | | 1992 | 1340275 |
| biochemical analysis of entamoeba histolytica hm1 strain resistant to complement lysis. | in this study, quantitation of several hydrolases and analysis of electrophoretic patterns of e. histolytica hm1 axenic strain periodically exposed to lytic-human serum (lytic-hs), heat-inactivated human (hi-hs) or bovine (hi-bs) sera were performed. the n-acetylglucosaminidase (nagase) levels increased in amebas resistant to complement. the acid phosphatase (ap) and proteolytic activity (pa) levels did not vary significantly in all groups of treated amebas, as compared with amebas from axenic c ... | 1992 | 1340276 |
| macrophage colony-stimulating factor enhances the respiratory burst of human monocytes in response to entamoeba histolytica. | mononuclear phagocytes (mp) are probably the most capable effector cells of the body in the defense against virulent strains of e. histolytica. killing of e. histolytica by mp appears to involve both oxidative and non-oxidative mechanisms. thus, in this study we have investigated whether trophozoites of an axenic virulent strain e. histolytica hm1:imss (eh) were capable of eliciting an oxidative response in pure populations of freshly isolated human monocytes. using a luminol-enhanced chemilumin ... | 1992 | 1340277 |
| the killing of entamoeba histolytica by activated human eosinophils. | unlike normal, opsonin aided human eosinophils, fmlp-activated human eosinophils are capable of destroying virulent e. histolytica. opsonins are not required for this action, although they enhance the effect. some activated eosinophils succumb in the action as well, probably victims of toxic products released by dying amebas. activated eosinophils thus appear to resemble activated macrophages in their dealing with this parasite. | 1992 | 1340278 |
| entamoeba histolytica modulates tnf-alpha, il-1 alpha/beta and c-fos gene expression in macrophages. | e. histolytica infections induce a state of transient suppression of cell-mediated immunity. as macrophages are involved in host defense in amebiasis, we determined whether soluble amebic lysates (eh) can modulate tnf-alpha, il-1 alpha/beta and c-fos gene expression in naive bone marrow-derived macrophages (bm delta). by northern analysis, the rna production of these genes after 0, 0.5, 1 and 3 h exposure to eh was determined and compared to lipopolysaccharide (lps) stimulation. in response to e ... | 1992 | 1340279 |
| further physicochemical characterization of the monocyte locomotion inhibition factor (mlif) produced by entamoeba histolytica. | ultrafiltration, gel-sieve chromatography and hplc were used to purify mlif. this material was hcl-hydrolyzed and the amino acids analyzed by hplc-ortho-ophthaldialdehyde. two hydrophobic non-polar (ile,leu), two uncharged polar (thr,tyr) and two positively charged (basic) (his, arg) amino acids were found in clear excess to their concentration in amc and thus may intervene in the composition of mlif. | 1992 | 1340280 |
| sex differences in systemic and local immune responses to entamoeba histolytica after intraperitoneal and rectal immunization in balb/c mice. | the antibody responses against entamoeba histolytica in peyer's patches and spleen after rectal, intraperitoneal and intragastric immunization with glutaraldehyde-fixed amebas (gfa) was compared between male and female mice by the use of a spot forming cell (sfc) assay. we found that female mice elicit significant higher anti-amebic sfc responses than male mice, in both spleen and peyer's patches after rectal and intraperitoneal stimulation, whereas by intragastric route females show higher resp ... | 1992 | 1340281 |
| effect of the monocyte locomotion inhibitory factor (mlif) produced by entamoeba histolytica upon the respiratory burst of human leukocytes. | in addition to inhibiting the locomotion of human mp, mlif appears capable of inhibiting the respiratory burst (measured by chemiluminescence) of mp and of pmn as well. the effect on the latter cells may or may not be relevant in the host-e. histolytica interaction, as pmn have been found to be notoriously inefficient in dealing with amebas and, foremost, do not use oxidative mechanisms in dealing with the parasite. on the other hand, the inhibitory effect on mp may represent a true evasion mech ... | 1992 | 1340282 |
| active immunization in hamsters with live entamoeba histolytica trophozoites of low virulence. | live trophozoites of entamoeba histolytica of low virulence (lv) were inoculated intraportally in hamsters. one week later they were challenged intrahepatically with highly virulent (hv) trophozoites of the same strain and sacrificed 1 week later. significant protection manifested by the production of smaller lesions was observed in immunized animals. antiamebic igg levels were significantly higher in the immunized group. histologically, livers of immunized animals presented small lesions contai ... | 1992 | 1340283 |
| kinetics of the anti-amebic antibody producing cells response in peyer's patches and spleen after both local and systemic stimulation in balb/c mice. | the kinetics of the anti-amebic antibody producing cell (apc) response was analyzed by a spot forming cell (sfc) assay, after a single dose of glutaraldehyde fixed amebas (gfa) by intragastric, rectal and intraperitoneal routes. it was found that mice elicited both local and systemic immune responses in peyer's patches and spleen, by either local or systemic stimulation. the analysis of the isotype of the anti-amebic apc in mice given four times the gfa by the mentioned routes revealed that mice ... | 1992 | 1340284 |
| identification of a parasite-specific cytoplasmic 67 kda entamoeba histolytica antigen recognized by patient sera and monoclonal antibodies. | to avoid false positive reactions in tests for anti-ameba antibodies, we wanted to identify parasite-specific component(s). amebiasis patient sera recognized an antigen of 67 kda by immunoblotting in an active e. histolytica fraction obtained by ion exchange chromatography. monoclonal antibodies against the fraction were made. antibody 3g2 reacted with three antigenic components of 67, 40 and 25 kda and in the immunocytology with an epitope located in the cytoplasm of e. histolytica trophozoites ... | 1992 | 1340285 |
| scanning electron microscopy of erythrophagocytosis by entamoeba histolytica trophozoites. | we analyzed by scanning electron microscopy (sem) the sequence of erythrocyte ingestion by a virulent strain of entamoeba histolytica trophozoites. axenic cultures of amebas were placed in chamber plastic slides for 30 min. human red blood cells (rbc) were added to the wells in a ratio of 1:100, ameba:erythrocytes. after different times of interaction, cells were fixed and processed for sem. erythrophagocytosis by e. histolytica is characterized by random suction of cells at early stages and pol ... | 1992 | 1340286 |
| phagocytosis and proteinase activity are not related to pathogenicity of entamoeba histolytica. | to examine the relationship between phagocytosis, proteinase activity and pathogenicity of axenically grown trophozoites of e. histolytica of strain hm-1:imss four different cultures were used: 1) a culture preserved in our laboratory for over 4 years, that lost its pathogenicity 3 years ago; 2) a culture passaged several times through hamster liver, that lost its pathogenicity recently; 3) a highly virulent culture supplied by another laboratory; and 4) amebas recovered from hamster liver absce ... | 1992 | 1340287 |
| a unique cysteine proteinase gene of pathogenic entamoeba histolytica correlates with virulence. | extracellular neutral cysteine proteinases are an important virulence factor of e. histolytica. experimental evidence supporting its role in invasion includes the ability to degrade components of the extracellular matrix and activate complement by specifically cleaving c3. we had previously reported the isolation of fragments encoding cysteine proteinase genes from hm-1 (acp1) and a nonpathogenic strain (ref291, acp2) by pcr using consensus sequences based on conserved structural motifs of eukar ... | 1992 | 1340288 |
| effect of intestinal bacteria on the virulence of entamoeba histolytica. | the effect on amebal virulence of escherichia coli, clostridium symbiosum and a mixture of lactobacilli was investigated. amebas from hm1 and hk axenic strains were incubated with a single bacterial strain or lactobacilli for short, intermediate, or long periods and analyzed for their erythrophagocytosis, hemolytic activity and cytotoxicity in vitro and hepatic abscesses formation in vivo. it was found that in vitro virulence was enhanced by all studied bacteria in hk9 but not in hm1 strains, wh ... | 1992 | 1340289 |
| cdna cloning of entamoeba histolytica histone h3. | e. histolytica has an unusual nuclear structure. although nucleosome-like particles were observed, no information about histones was available so far. in this paper we describe a cdna clone with significant homology to h3 histones that was isolated from a lambda zap library of pathogenic e. histolytica. the complete cdna encodes a 15 kda polypeptide, which is shorter by one residue than the human homologue. the amino acid sequence has only 69% identity with human h3.3 histone. our results indica ... | 1992 | 1340291 |
| immediate cell membrane damage produced by entamoeba histolytica subcellular extracts. | the alpha[14c]amino isobutyric acid is a synthetic amino acid with low molecular weight, not incorporated to protein. chinese hamster ovary (cho) cells were labeled with this radioactive compound and treated with the amebal subcellular fraction p30 at short times. we detected membrane damage in cho cells by specific radiolabel release from the first 30 sec of interaction between cell cultures and amebal fraction. | 1992 | 1340292 |
| interaction of various entamoeba histolytica strains with human intestinal cell lines. | the interaction of four pathogenic and three nonpathogenic e. histolytica strains with two human intestinal cell lines (caco-2 and ht-29) was examined. the adherence of pathogenic and nonpathogenic e. histolytica to these cells was similar, indicating that defective adherence to intestinal cells is not a common feature of nonpathogenic strains. the addition of different carbohydrates confirmed the importance of the galactose-binding lectin of e. histolytica in binding to these intestinal cells. ... | 1992 | 1340293 |
| model of intestinal amebiasis: structural and functional lesions to the rabbit colon mucosa by entamoeba histolytica lysates. | although the events occurring during the initial interaction of e. histolytica trophozoites with the mucosa of the large intestine probably determine the invasion by the parasites, an appropriate experimental model does not exist. to develop such a model we used full-thickness rabbit colon preparations (0.28 cm2) mounted in ussing-type chambers. untreated preparations had electrophysiological properties (potential difference, short-circuit current and electrical resistance) similar in magnitude ... | 1992 | 1340294 |