| the effect of giardia lamblia trophozoites on lipolysis in vitro. | infection with giardia lamblia often causes only minor mucosal changes to the small intestine yet frank fat malabsorption may still occur. some evidence suggests abnormal pancreatic exocrine function in subjects with giardiasis although the mechanism and significance of this is unclear. studies were conducted in vitro to determine the effect of g. lamblia trophozoites or culture filtrates from the organism on lipolysis of triglyceride by porcine pancreatic lipase. live trophozoites significantly ... | 1991 | 1945523 |
| prevalence of trichomonas vaginalis in men at high risk for sexually transmitted diseases. | this study determined the prevalence of trichomonas vaginalis in young men who were at high risk for sexually transmitted diseases; compared different diagnostic tests for trichomonads; and compared sexual behavior of men with positive and negative trichomonas test results. men (85) aged 16-22 years inclusive, were recruited from a job-training program to participate in this study. urethral specimens were obtained after prostatic massage for the isolation of neisseria gonorrhoeae, chlamydia trac ... | 1991 | 1948509 |
| medical health care for viennese prostitutes. | in vienna, legalized prostitution is tightly controlled by the advisory board of the viennese public health service. registered prostitutes are routinely screened for all important stds, such as syphilis, hiv, gonorrhea, chlamydial- and yeast-infections, and trichomonas vaginalis. furthermore, cytological smears are obtained from the cervix and chest x-rays are performed at least once a year. in all pathological findings, an appropriate therapy is implemented. presenting data of 1989, out of the ... | 1991 | 1948514 |
| adjunctive clindamycin therapy for preterm labor: results of a double-blind, placebo-controlled trial. | a double-blind, placebo-controlled, randomized trial was conducted to evaluate the efficacy, safety, and tolerance of a course of clindamycin (administered for 3 days intravenously and 4 days orally) among hospitalized women with preterm labor at less than or equal to 34 weeks' gestation who were treated with tocolytics. one hundred three woman-perinate pairs were analyzed. univariate analysis demonstrated that pregnancies were continued longer in women treated with clindamycin than in women who ... | 1991 | 1951545 |
| purification and characterization of methionine gamma-lyase from trichomonas vaginalis. | methionine gamma-lyase (ec 4.4.1.11) was purified to homogeneity from the anaerobic protozoan parasite trichomonas vaginalis by a series of f.p.l.c. procedures. the enzyme catalyses alpha gamma- and alpha beta-elimination reactions of a number of derivatives of methionine and cysteine. it also catalyses gamma-replacement reactions of the thiomethyl group of methionine, homocysteine and ethionine to yield the corresponding s-substituted homocysteine derivative. the enzyme is pyridoxal 5'-phosphat ... | 1991 | 1953661 |
| the parasitic flagellates trichomonas vaginalis and tritrichomonas foetus produce indole and dimethyl disulphide: direct characterization by membrane inlet tandem mass spectrometry. | the use of a membrane inlet triple quadrupole mass spectrometer revealed indole as an end product in the growth medium of cultures of the cattle parasite tritrichomonas foetus and the human parasite trichomonas vaginalis: formation of indole is enhanced in the presence of added tryptophan. two different clinical isolates of trich. vaginalis also produce dimethyl disulphide. electron impact ionization yielded complex fragmentation mixtures, but the facility for analysis of daughter ions enabled u ... | 1991 | 1955862 |
| chemorepulsion of trichomonads by products of neutrophil oxidative metabolism. | to determine whether secreted neutrophil products affect the migration of motile microorganisms such as trichomonas vaginalis, stimulated human neutrophils and cell-free oxygen metabolites were used as stimuli in a multiwell filter chemotaxis assay using tritiated t. vaginalis. when stimulated neutrophils were present on the opposite side of the filter, migration of t. vaginalis into the filter was significantly diminished, and this reduction varied with the dose of neutrophil stimulus. the redu ... | 1991 | 1984465 |
| no detection of characteristic fungal protein elongation factor ef-3 in pneumocystis carinii. | the taxonomic status of pneumocystis carinii is uncertain, and p. carinii has been categorized both as a fungus and as a protozoan. recent comparisons of rna sequence homologies between p. carinii and several genera of fungi and protozoa suggest that p. carinii has closer affinities with the ascomycetes than with the protozoa. the translatory systems of the fungi, however, require three soluble protein factors for peptide chain elongation rather than the two necessary in other eukaryotic systems ... | 1991 | 1995744 |
| the epidemiology of group b streptococcal colonization in pregnancy. vaginal infections and prematurity study group. | risk factors for cervicovaginal group b streptococcal colonization at 23-26 weeks' gestation were studied in 7742 women participating in the vaginal infections and prematurity study. the prevalence of group b streptococcus was 18.6%, and was greatest in (predominantly caribbean) hispanics from new york city, followed by blacks, whites, and other (predominantly mexican) hispanics. group b streptococcus was more common among older women and women of lower parity, and less common among women living ... | 1991 | 2002986 |
| antepartum cultures for ureaplasma urealyticum are not useful in predicting pregnancy outcome. the vaginal infections and prematurity study group. | to test the hypothesis that genital colonization with ureaplasma urealyticum would predict adverse pregnancy outcome, 4934 women from five medical centers were evaluated for vaginal colonization with u. urealyticum between 23 and 26 weeks' gestation and followed up to delivery. u. urealyticum colonization was associated with maternal age, parity, racial-ethnic group, martial status, income, education, smoking, number of sexual partners, and colonization with trichomonas vaginalis, mycoplasma hom ... | 1991 | 2003532 |
| phospholipid metabolism of cultured trichomonas vaginalis and tritrichomonas foetus. | trichomonas vaginalis and tritrichomonas foetus grown in a fetal calf serum-based culture medium were exposed to radiolabeled phospholipids and lipid precursors to determine the extent to which these organisms can incorporate complex lipids and/or de novo synthesize their major membrane phosphoglycerides. phosphatidylethanolamine and phosphatidylcholine were the dominant phospholipids (40-50% of extractable phospholipids), with acidic lipids, phosphatidylinositol, phosphatidylserine, phosphatidy ... | 1991 | 2011157 |
| sexually transmitted parasitic diseases. | sexual activity is the primary method of transmission for several important parasitic diseases and has resulted in a significant prevalence of enteric parasitic infection among male homosexuals. the majority of parasitic sexually transmitted diseases involve protozoan pathogens; however, nematode and arthropod illnesses are also included in this group. trichomoniasis, caused by trichomonas vaginalis, is the most common parasitic std. infection with this organism typically results in the signs an ... | 1991 | 2011632 |
| correlation of zymodeme patterns, virulence & drug sensitivity of trichomonas vaginalis isolates from women. | characterization of t. vaginalis strains isolated from symptomatic and asymptomatic subjects was done by isoenzyme analysis. the pathogenicity of these isolates was checked in mouse model and in vivo drug susceptibility was determined. the isolates from symptomatic and asymptomatic individuals could not be grouped on the basis of isoenzyme analysis alone. all the strains except one, were pathogenic for mice, and metronidazole (50 mg/kg body weight) was effective in protecting the mice from t. va ... | 1991 | 2022400 |
| epidemiology of trichomonas vaginalis (tv) in rural adolescent and juvenile children. | | 1991 | 2027176 |
| an evaluation of an inpouch tv culture method for diagnosing trichomonas vaginalis infection. | a new culture method for trichomonas vaginalis, the inpouch tv test, was evaluated for its sensitivity and specificity in supporting growth of trichomonads. five clinical isolates remained viable for periods from 41 to 131 days. a strain from the attc 30001 remained viable for 91 days. as few as four trichomonads/ml of culture medium could be viewed microscopically within 24 h. doubling time for growth of trichomonad varied between 5 to 8 h. in a clinical study of 102 wet-mount negative specimen ... | 1991 | 2032710 |
| dna single strand-breaks in lymphocytes after metronidazole therapy. | dna single strand-breaks were examined in lymphocytes of patients treated with metronidazole (cas 443-48-1) for trichomonas vaginalis infection before, on day one, and day seven of treatment. recommended dosage of metronidazole induces dna single strand-breaks. single strand-breaks were repaired after therapy. no irreversible dna damage remains. the dna repair mechanisms are genetically determined. therefore a mutagenic effect of metronidazole must be discussed in patients with dna repair defect ... | 1991 | 2043178 |
| [epidemiology and diagnostics in the vaginal area. i. study of the vaginal flora using microscopy and evaluation of the microbial picture of the vagina]. | the microscopic characteristic of the vaginal microflora by means of microbial vaginal smears (mvs) according to jírovec, peter and málek corresponds no longer to contemporary ideas on the physiology and pathology of the vaginal environment. the authors revealed on comparison of the results of mvs and the native preparation that in a group of 600 women selected at random the mvs diagnosis allowed to escape 80 cases of trichomoniasis (46.8%), 56 cases of mycotic infections (34.1%) and cases of su ... | 1991 | 2044156 |
| vaginal infection and preterm labour. | to study the vaginal flora of women in preterm labour (ptl) and determine whether the presence of specific vaginal microflora is significantly associated with onset of ptl. | 1991 | 2059587 |
| intestinal parasitoses and other infections in a college community. | in order to assess the health status of students doing their teaching diploma course at the siriba teachers college, maseno, a study of their blood slides, their stools for ova and cysts and their urine samples for urinary tract infection was carried out. a total of 298 students were investigated: 27 (6%) had intestinal parasites, 96 (32.2%) had urinary tract infection, i.e., pyuria, bacteria and epithelial cells in their urines, most of them being females (55/96 or 57.3%). the peak occurrence o ... | 1991 | 2060482 |
| the interaction of tritrichomonas foetus and trichomonas vaginalis with macrophages. | the process of interaction between macrophages and tritrichomonas foetus and trichomonas vaginalis was analysed using light microscopy, scanning and transmission electron microscopy. the parasites attach to the macrophage surface and are ingested through a phagocytic process. parasite-macrophage association index was higher for activated than for resident macrophages. previous incubation of the parasites in the presence of concanavalin a rendered their surface less negative and more hydrophobic, ... | 1991 | 2070357 |
| vaginal antibody of patients with trichomoniasis is to a prominent surface immunogen of trichomonas vaginalis. | twenty vaginal washes (vws) and ten vaginal mucus (vm) samples from patients with trichomoniasis were examined for the presence of antibody to surface protein immunogens of trichomonas vaginalis. fourteen of 20 vws (70%) and 8 of 10 vm (80%) had immunoglobulin g (igg) antibody (ab) that reacted in an immunoprecipitation (ip) assay with one iodinated trichomonas vaginalis surface protein immunogen with a relative molecular mass of 230,000 daltons (230-kda) (p230). no similar ip of any iodinated p ... | 1991 | 2071124 |
| glutathione transferase activity in some flagellates and amoebae, and purification of the soluble glutathione transferases from acanthamoeba. | glutathione transferase (gst) enzymes are toxicologically important from many points of view. nine protozoans were investigated here for their gst content. six aerobic amoebae had very different specific gst activities, but an anaerobic amoeba and two anaerobic flagellates did not have any gst activity, suggesting that the peroxidase activity of gst is an evolutionarily important property for aerobic organisms. the soluble gst isoenzymes of acanthamoeba culbertsoni and a. polyphaga were purified ... | 1990 | 2078188 |
| [antiprotozoal effects of benzodiazepine derivatives]. | it is known that some derivatives of the benzodiazepine group act not only as antipsychotic drugs but also have inhibitory effects on the growth of protozoa. the influence of imipramine and its derivatives clomipramine and desipramine on the multiplication of crithidia luciliae, leishmania mexicana amazonensis, trypanosoma cruzi and trichomonas vaginalis was investigated using in vitro cultivation of the parasites and compared with the effects of chlorpromazine and metronidazole. all trypanosoma ... | 1990 | 2085212 |
| sexually transmitted diseases and rape: the experience of a sexual assault centre. | epidemiological data and rates of sexually transmitted diseases (stds) are presented from a group of 90 women screened following rape. forty-eight (53%) women knew their assailant. in 6 cases multiple assailants were involved. in 21 (23%) rapes weapons were used. of 47 women examined within 48 hours of rape, 17 (36%) had evidence of genital trauma. anal injury was common (8 of 11, 73%) in those reporting anal intercourse. anal assault (17 of 90, 19%) and oral assault (16 of 90, 18%) commonly acc ... | 1990 | 2094402 |
| a cytotoxic effect of h2o2 on trichomonas vaginalis may be correlated to cytoskeleton modification. | | 1990 | 2097657 |
| treatment failure in trichomoniasis and persistence of the parasite after lactobacillus immunotherapy; two case reports. | this report describes the clinical and laboratory observations on two patients with a trichomonas vaginalis infection resistant to metronidazole. the metronidazole resistance was confirmed in in vitro cultures under aerobic conditions, after in vitro cultivation of the strains. trichomonas infection persisted during high-dose intravenous metronidazole administration and lactobacillus immunotherapy was unsuccessful in both patients. | 1990 | 2105899 |
| pattern of genito-urinary parasitic infections at the teaching hospital, sokoto, nigeria. | urinary schistosomiasis was more frequently observed among subjects aged 10-19 years old; and the degree of exposure to the infection was found to be high among the males. comparative analysis of t. vaginalis infection among the females revealed a ten-fold increase in the detection rate for the flagellate in the urine samples collected from the special treatment clinic than those from out-patient department. the age-specific morbidity pattern for trichomonal vaginitis was remarkably high among f ... | 1990 | 2112996 |
| a microbiological study of failed penicillin therapy for gonococcal urethritis in durban. | seventy-five men presenting with persistent urethral discharge after penicillin therapy were investigated for sexually transmitted pathogens during july - september 1987. the major aetiological agent isolated was neisseria gonorrhoeae (58 patients (77.3%]. penicillinase-producing n. gonorrhoeae (ppng) accounted for 58.6% of 58 isolates. chlamydia trachomatis was detected in 10.7% of patients and trichomonas vaginalis in 14.7% of patients. when there is a high prevalence of ppng, the use of penic ... | 1990 | 2116671 |
| trichomonas vaginalis requires traces of oxygen and high concentrations of carbon dioxide for optimal growth. | the effects of o2 and co2 on the growth in culture of trichomonas vaginalis strain c1-nih were investigated. growth under pre-purified n2 in the absence of co2 supplementation gave a doubling time of 4.4 h; when traces of o2 (less than 0.25 microm) were present, the doubling time was 3.5 h. organisms grew most rapidly (doubling time 2.3 h) with traces of o2 (less than 0.25 microm) and with the co2 level controlled at 5 mm. the balance of fermentation products from maltose was greatly influenced ... | 1990 | 2117256 |
| a further look at infection at the time of therapeutic abortion. | microbiological investigations and clinical follow-up were performed on 516 patients undergoing first-trimester termination of pregnancy, over a 2-year period. chlamydia trachomatis was isolated by pre-operative microbiological screening in 6.7% of patients, gonococcus in 0.4% and trichomonas vaginalis in 2%. candida spp and gardnerella vaginalis were isolated from 16% and 28% of the women, respectively. patients who had positive cervical chlamydial isolation were significantly younger (less tha ... | 1990 | 2121562 |
| neisseria gonorrhoeae, trichomonas vaginalis, and yeast reported from attenders at an antenatal clinic in a rural area in botswana. | | 1990 | 2125025 |
| in vitro antimicrobial activity of econazole and miconazole sulfosalicylate. | the in vitro activities of new sulfosalicylic salts of econazole (e.ssa) and miconazole (m.ssa) have been investigated in comparison with the respective nitrate (nit) salts and sulfosalicylic acid (ssa) alone. the results reveal good antimicrobial activity of m.ssa and e.ssa against different strains of candida, dermatophytes, moulds, gram-positive bacteria and trichomonas vaginalis. the mic values demonstrate that m.ssa is more active than m.nit on candida and t. vaginalis. e.ssa was also more ... | 1990 | 2127740 |
| effects of berberine, a plant alkaloid, on the growth of anaerobic protozoa in axenic culture. | extracts obtained by organic solvents from the root of coptis teeta in myanmar (burma), were tested for growth inhibitory activity against giardia lamblia, trichomonas vaginalis and entamoeba histolytica in axenic culture. all extracts had anti-protozoal activity with the methanol extract, in particular, being effective against all 3 parasites. by chromatographic analysis, the extracts were shown to contain berberine compounds and other alkaloids. comparing the inhibitory effects of the methanol ... | 1990 | 2131648 |
| [structure and evolution of trichomonadida]. | in the article the survey of data on morphology and ultrastructure of flagellates of the order trichomonadida is given with special attention paid to monocercomonadidae and trichomonadidae. the evolution within these flagellates is considered as a series of changes leading to formation of undulating membrane and reinforcement of the cytoskeleton. in both families other two evolutionary tendencies may be also observed: reduction of the flagellar apparatus (in monocercomonadidae) and enlargement o ... | 1990 | 2131689 |
| [effect of pseudomonas aeruginosa on trichomonas vaginalis in vitro--electron-microscopic studies]. | interaction between t. vaginalis and p. aeruginosa was studied in vitro. protozoan cells were incubated in 0.9% nacl at 37 degrees c for 30 and 150 min. with bacteria, alive or killed by heating for 10 min. at 100 degrees c. transmission electron microscope observations showed weak ++phagocytic activity (less than 10%) of t. vaginalis against living p. aeruginosa. phagocytosis of these bacteria caused death of t. vaginalis. disintegration and autolysis of t. vaginalis resulted probably from acti ... | 1990 | 2131691 |
| [incidence of trichomonas vaginalis infection among girls from the lódź population in the last 25-year period]. | 19,024 girls (aged: from 0 to 18) attending the pediatric gynaecology outpatient department and the center for treatment of parasitic and mycotic diseases were examined. the diagnosis of infection with t. vaginalis was based on preparations using various methods and cultures on specific media, from vaginal content and urine. it was found that the frequency of t. vaginalis invasions in girls had lessened 2-6 times in different groups from 1960 till 1985 and the higher percent of t. vaginalis in g ... | 1990 | 2131692 |
| [several years of observation of the incidence of trichomonas vaginalis infections among female workers in a single industrial plant]. | during 5 years cycle a prophylactic gynaecological, cytological and parasitological examinations of 1918 women were performed. 12.8% of women were infected with t. vaginalis. decreasing percentage of infections was observed: from 9.92% in 1984 to 2.2% in 1988. in the i. of papanicolaou cervical smears t. vaginalis has been found in 1.60% of cases, in the ii.--in 5.2%, in the iii.--in 14.29%, and in the iv.--in 22.22% of cases. in women with normal cervix uteri t. vaginalis have been found in 5.0 ... | 1990 | 2131693 |
| [trichomonas vaginitis at different life stages of women]. | using of fresh slides and culture t. vaginalis was found in 1094 persons (3.20%). it has been seen the morphological differentiation of t. vaginalis connected with the age, physiological state of macroorganisms and the clinic picture of trichomoniasis. in vivo, the spheroidal as well as ameboid forms of t. vaginalis were observed. the latter ones, characteristic in acute trichomoniasis, were often seen in pregnant women. on the other hand, nonmobile round-shaped forms of t. vaginalis occurred in ... | 1990 | 2131695 |
| [analysis of the relations between selected symptoms of inflammation of the genital and urinary organs and invasion of trichomonas vaginalis]. | data concerning 604 girls up 18 year old were analysed. on the basis clinical symptoms and results of culture of various biological materials on selected media--the monofocal trichomonas infection concerning the most often vulva and vagina, rarely urethra or other regions of urinary organ as well as the multifocal trichomonas infection, particularly complicated by mycosis were proved. it was found the convergence statistically significant (p less than or equal to 0.5) of the invasion of t. vagin ... | 1990 | 2131696 |
| [rare cases of prostatitis caused by invasion of trichomonas vaginalis with candida albicans]. | three cases of prostatitis caused by the invasion of t. vaginalis and c. albicans which has been found in different biological materials were described. after per rectum examination perineum biopsy of prostata was performed in all patients; in histopathological preparations pointing at the inflammation reaction of that gland the fungi were detected. also, the same microorganisms were proved in sexual partners of those patients but multifocal invasion of c. albicans--including genital and urinary ... | 1990 | 2131697 |
| [studies of compounds with potential antiparasitic action. viii. new oxime ethers in the 1-thiochromone system]. | the 50% antitrichomonal concentration of 7 examined ethers (t. vaginalis strain n. 1/86, roiron medium, safranine staining, 30 min.) was estimated by means of dose-response curve. the minimal mycostatic concentration (candida albicans l-45, geotrichum candidum; 3% sabouraud agar, 37 degrees c, 24 hr) was calculated with the aid of regression equation. all examined ethers show in vitro a marked antitrichomonal effect comparable with activity of ornidazole or phenol. mycostatic activity of new com ... | 1990 | 2131701 |
| [antitrichomonal properties of derivatives of ethers containing amine and halogenarylalkyl groups]. | the trichomonacidal activity in vitro of 6 new derivatives of theirs containing amine and halogenaryloalkyl groups in comparison with metronidazole, ornidazole and tinidazole has been examined. for all the examined compounds curves of action were obtained after 30 min, and lethal concentrations for 50% population (cl50) of three strains of t. vaginalis used for experiments were calculated. the trichomonacidal action of the following compounds was specially strong: 2-/1-methylpiperazine++-4-yl/et ... | 1990 | 2131703 |
| [in vitro sensitivity of entamoeba histolytica to metronidazole]. | the chemotherapy of amebiasis includes different substances with intra and extraintestinal action. nowadays, metronidazole is the election drug by its high efficacy and low toxicity. some therapeutic failures with metronidazole in patients with invader amebiasis and some reports of resistance to it, of certain strains of trichomonas vaginalis and species of bacteroides have caused an increased interest to do new in vitro evaluation of this compound and to search of new antiamebic drugs. the obje ... | 1990 | 2136490 |
| fibronectin- and laminin-mediated endocytic activity in the parasitic protozoa trichomonas vaginalis and tritrichomonas foetus. | the pathogenic protozoan tritrichomonas foetus is able to ingest polystyrene particles with a diameter up to 1.0 micron. trichomonas vaginalis, however, ingest particles as large as 4.4 microns in diameter. the particles are found within cytoplasmic membrane-bounded vacuoles. morphometrical analysis showed that t. vaginalis presents a higher endocytic activity than t. foetus. coating of the polystyrene particles with cationized ferritin increases their binding to the parasite surface but does no ... | 1990 | 2138055 |
| structural and histoenzymatic aspects of metronidazol on rat gonads. | metronidazol, an efficient drug for trichomonas vaginalis, lamblia intestinalis and some anaerobic germs, administered in a daily dose of 500 mg/kg body weight for 60 and 40 days in males and females, respectively, produces severe structural alterations (atrophy and cytolysis, more apparent in the testicle) and elicits, a functional impairment as well (modifications in the activity of some enzymes: alkaline and acide-phosphatase, mg(2+)-atpase, cyox, sdh, ldh). these morphological and histoenzym ... | 1990 | 2151289 |
| freeze-fracture study of trichomonas vaginalis. | the freeze-fracture technique was used to analyse the organization of the plasma membrane, as well as membranes of cytoplasmic organelles, of the pathogenic protozoan trichomonas vaginalis. rosettes formed by 4 to 14 intramembranous particles were seen on the fracture faces of the membrane lining the anterior flagella as well as in fracture faces of the plasma membrane enclosing the anterior region of the protozoan and in cytoplasmic organelles. special organization of the membrane particles wer ... | 1990 | 2152194 |
| sexually transmitted diseases in victims of rape. | the risk of acquiring a sexually transmitted disease as a result of rape is not known, in part because it is difficult to ascertain whether infections were present before the assault or acquired during it. to investigate this question, we examined female victims of rape within 72 hours of the assault and again at least one week after the assault. of the 204 girls and women initially examined within 72 hours of the rape, 88 (43 percent) were found to have at least one sexually transmitted disease ... | 1990 | 2155389 |
| cytomegalovirus infection among women attending a sexually transmitted disease clinic: association with clinical symptoms and other sexually transmitted diseases. | the prevalence rates of cytomegalovirus, neisseria gonorrhoeae, chlamydia trachomatis, trichomonas vaginalis, and herpes simplex virus infection were determined for 247 women attending a sexually transmitted disease clinic in halifax, nova scotia between july 1983 and december 1985. isolation rates were 8.5%, 32.8%, 27.1%, 7.3%, and 6.5% for the five infectious agents, respectively. with multiple logistic regression analysis, the presence of cervical cytomegalovirus infection was independently a ... | 1990 | 2156421 |
| inhibition of trichomonas vaginalis motility by monoclonal antibodies is associated with reduced adherence to hela cell monolayers. | adherence of trichomonads to host epithelial cells appears to be a critical step in the pathogenesis of trichomoniasis. we evaluated the effect of a panel of 10 monoclonal antibodies on attachment of [35s]methionine-radiolabeled trichomonas vaginalis strains to hela cell monolayers. of 10 monoclonal antibodies, 3 totally eliminated motility of phs2j strain trichomonads and reduced their adherence to 48 to 60% of control values (p less than 0.001). however, none of the monoclonal antibodies affec ... | 1990 | 2187803 |
| inhibition of pyruvate:ferredoxin oxidoreductase from trichomonas vaginalis by pyruvate and its analogues. comparison with the pyruvate decarboxylase component of the pyruvate dehydrogenase complex. | pyruvate:ferredoxin oxidoreductase and the pyruvate dehydrogenase multi-enzyme complex both catalyse the coa-dependent oxidative decarboxylation of pyruvate but differ in size, subunit composition and mechanism. comparison of the pyruvate:ferredoxin oxidoreductase from the protozoon trichomonas vaginalis and the pyruvate dehydrogenase component of the escherichia coli pyruvate dehydrogenase complex shows that both are inactivated by incubation with pyruvate under aerobic conditions in the absenc ... | 1990 | 2188649 |
| genital infections in women attending a genito-urinary clinic in harare, zimbabwe. | one hundred women attending a sexually transmitted diseases clinic in harare were examined for presenting features and genital infections. the most common presenting symptoms were of discharge, lower abdominal pain and dysuria, and on examination signs of discharge, inflammation, haemorrhage or ulcers/erosions were noticeable in all women. fourteen women had genital warts. pathogens were detected in 95% of patients. gonococcal infection occurred in 19 women, with 60% of the strains isolated bein ... | 1990 | 2196216 |
| cdna sequence analysis of a 29-kda cysteine-rich surface antigen of pathogenic entamoeba histolytica. | a gamma gt11 cdna library was constructed from poly(u)-sepharose-selected entamoeba histolytica trophozoite rna in order to clone and identify surface antigens. the library was screened with rabbit polyclonal anti-e. histolytica serum. a 700-base-pair cdna insert was isolated and the nucleotide sequence was determined. the deduced amino acid sequence of the cdna revealed a cysteine-rich protein. dna hybridizations showed that the gene was specific to e. histolytica since the cdna probe reacted w ... | 1990 | 2201027 |
| treatment of sexually transmitted vaginosis/vaginitis. | although much has been learned about sexually transmissible forms of vaginitis/vaginosis during the past decade, therapeutic options for these disorders remain limited. a single 2-g oral dose of metronidazole still remains highly effective against most trichomonas vaginalis infections. mildly resistant infections can usually be cured with metronidazole oral doses of 2 g daily for 3-7 days. when highly resistant infections occur, toxic levels of metronidazole may be needed for the drug to cure th ... | 1990 | 2201078 |
| glucokinase and fructokinase of trichomonas vaginalis and tritrichomonas foetus. | trichomonas vaginalis and tritrichomonas foetus contain glucokinase and not a hexokinase of broad hexose specificity. tritrichomonas foetus also contains a specific fructokinase which could be resolved from glucokinase by anion exchange chromatography. native t. vaginalis glucokinase had a mr of 76,000, and sds-pag electrophoresis showed two equally stained bands corresponding to mr 40,000 and 38,000. glucose and atp were by far the best substrates for both trichomonad glucokinases, with km valu ... | 1990 | 2213652 |
| [bound amino acids in local strains of trichomonas vaginalis]. | amino acid composition of water-soluble and water-insoluble proteins of 8 strains of tr. vaginalis is studied. 17 amino acids are found in both protein hydrolyzates. despite the complete coincidence of their qualitative compositions there are reliable differences in the quantitative contents of some amino acids. differences in the contents of main amino acids of water-soluble proteins of different strains reflect the belonging of the latter to different sero-groups. no reliable differences in th ... | 1990 | 2216532 |
| prevalence of gonorrhoea, syphilis and trichomoniasis in prostitutes in burkina faso. | prevalence of syphilis, gonorrhoea and trichomoniasis among 127 prostitutes in ougadougou, burkina faso, was investigated. 22% were positive of syphilis, 20% for intracellular gram negative diplococci, and 17% for trichomonas vaginalis infection. demographic data revealed that the prostitutes were a young group, with 69% between 15 and 25 years old. 14% of the women were from burkina faso; the majority (73%) were of ghanaian origin. our data highlight alarming rates of stds in prostitutes workin ... | 1990 | 2226226 |
| reduced lymphocyte responses to mitogens in natural and experimental trichomoniasis. | proliferative responses to mitogens were determined by using peripheral blood mononuclear cells from women with active trichomoniasis, with serological evidence of past infection with trichomonas vaginalis, and with no evidence of current or past infection. even after the human immunodeficiency virus antibody status of the patients was taken into account, cells from women with active trichomoniasis showed reduced responses to phytohemagglutinin, concanavalin a, pokeweed mitogen, and bacterial li ... | 1990 | 2228226 |
| antioxidant enzymes in trichomonas vaginalis. | the activities of artificial donor peroxidase, catalase, and superoxide dismutase in clinical isolates of t. vaginalis were studied. none of the tested strains exhibited activity of the three enzymes. inclusion of catalase in diamond-tym solid medium did not support growth under aerobic conditions. the correlation between the absence of these enzymes and the growth conditions of the organism is discussed. | 1990 | 2230334 |
| association of production of cell-detaching factor with the clinical presentation of trichomonas vaginalis. | recent work has shown that trichomonas vaginalis produces a cell-detaching factor (cdf) that causes detachment of monolayer cells in vitro. to study the role of cdf as a pathogenic marker of disease, we studied the production of cdf in 12 clinical isolates of t. vaginalis. these isolates were also utilized in the mouse subcutaneous assay of honigberg, which is the standard for pathogenicity of t. vaginalis. the isolates were divided into three groups based on clinical presentation (asymptomatic ... | 1990 | 2254417 |
| growth of trichomonas vaginalis in a serum-free mccoy cell culture system. | axenic cultures of trichomonas vaginalis normally require serum for proliferation, yet serum-containing medium may interfere with the detection of t. vaginalis-secreted virulence factors. trichomonas vaginalis can, however, grow in coculture with a mccoy cell monolayer in both the presence and absence of serum. for 6 t. vaginalis isolates examined, growth in this serum-free system shows lower peak concentrations of t. vaginalis and longer doubling times than those apparent in a serum-containing ... | 1990 | 2254829 |
| characterization of trichomonas vaginalis haemolysis. | the haemolytic activity of live trichomonas vaginalis organisms was investigated. optimal haemolysis of human erythrocytes was observed at a parasite to erythrocyte ratio of 1:5 during a 2 h incubation period. no haemolytic activity was detected in concentrated culture supernatants after overnight growth of trichomonads or when parasites were separated from erythrocytes by a 3 microns filter, suggesting a contact-dependent mechanism for haemolysis. the haemolytic activity was temperature-depende ... | 1990 | 2263411 |
| sexually transmitted diseases in rape victims. | from 1 january 1986 to 1 september 1989 124 women presented to the ambrose king centre (the department of genitourinary medicine of the london hospital) alleging rape. sexually transmitted diseases were found in 36 (29%) women (excluding candidosis and bacterial vaginosis). the commonest organisms detected were neisseria gonorrhoeae and trichomonas vaginalis, each being present in 15 patients. eleven women had genital warts. chlamydia trachomatis was isolated in six patients, two had herpes simp ... | 1990 | 2265841 |
| dot-immunobinding assay with monoclonal antibody for detection of trichomonas vaginalis in clinical specimens. | a rapid and specific dot-enzyme immunoassay (diba) using monoclonal antibody was introduced for detection of trichomonas vaginalis antigen in vaginal and urethral materials. the results of diba were compared with culture findings of the parasite in 245 female patients. taking culture as the reference method, diba had a sensitivity of 92% and a specificity of 93%. the predictive value of a positive test was 98% and that of a negative test 77%. the efficacy of wet mount, culture and diba were comp ... | 1990 | 2265844 |
| [detection of igg and igm antibodies with elisa technique in human trichomoniasis]. | the direct wet mount examination of vaginal secretion, widely applied for the diagnosis of trichomonas vaginalis infection in woman patients, is rapid and economical, however, the sensitivity of this technique is not so high. in this study enzyme-linked immunosorbent assay (elisa) was employed for the detection of serum anti-t. vaginalis igg and igm antibodies from 30 vaginal trichomoniasis patients and 30 non-infected healthy persons. the results were as follows: 1. serum elisa-igg value was 0. ... | 1990 | 2271497 |
| [cytotoxicity of resident and lymphokine-activated mouse peritoneal macrophage against trichomonas vaginalis]. | this study was aimed to observe the direct and lymphokine-activated cell mediated cytotoxic effects against trichomonas vaginalis by mouse peritoneal macrophages. cytotoxicity was measured as release of 3h-thymidine from prelabeled protozoa, and tested in u-bottom microtiter plates. a 0.1 ml suspension of labeled protozoa (2 x 10(5)/ml) was placed in each well, followed by 0.1 ml of a suspension containing increasing numbers of peritoneal cells. after a 24 hr incubation at 37 degrees c, 0.1 ml o ... | 1990 | 2271505 |
| [a method for the long-term preservation of trichomonas vaginalis strains]. | | 1990 | 2278206 |
| [the antitrichomonal activity of the sodium salts of anthranilic acid]. | | 1990 | 2278315 |
| characteristics of trichomonas vaginalis isolates from women with and without colpitis macularis. | colpitis macularis, the most specific clinical sign for diagnosis of trichomoniasis, was detected by colposcopy in 52 of 118 infected women. patients with colpitis macularis had a mean (+/- sd) of 18 +/- 20 trichomonas vaginalis organisms/field (x 400 magnification) compared with 7 +/- 17 in patients with colpitis macularis (p less than .003). to compare possible virulence markers in isolates from women with and without colpitis macularis, isolates were analyzed for hemolytic activity, abscess p ... | 1990 | 2299210 |
| transvaginal-peritoneal migration of trichomonas vaginalis as a cause of ascites. a report of two cases. | genital tract colonization with trichomonas vaginalis usually results in vaginitis without systemic manifestations. there are only four recorded cases of trichomonas recovered from pelvic organs or peritoneal fluid. this report describes two additional women in whom motile flagellate organisms were recovered from abdominopelvic ascites. we hypothesize that an ascending trichomonal infection may have played an etiologic role in the development of their acute disease. | 1990 | 2304041 |
| a prospective study of genital infections in a family-planning clinic. 1. microbiological findings and their association with vaginal symptoms. | a prospective study of genital infection was conducted in four inner-city family-planning clinics. fifteen per cent of routine attenders had symptoms and signs of vaginal infection and many more women attended primarily because of symptoms. among the women with both signs and symptoms, 70% had positive laboratory findings, trichomonas vaginalis, candida albicans and bacterial vaginosis being equally prevalent. measurement of vaginal ph in the clinic was the single most useful clinical finding fo ... | 1990 | 2307184 |
| the specificity of trichomonad cysteine proteinases analysed using fluorogenic substrates and specific inhibitors. | the multiple cysteine proteinases of trichomonas vaginalis and tritrichomonas foetus, both those retained intracellularly and those released, were separated using gelatin-sds-page, and their activity towards a range of 15 fluorogenic peptidyl aminomethylcoumarins determined together with their relative sensitivity to inhibitors. three types of enzyme were apparent in t. vaginalis: (i) an 86-kda enzyme active only on z-arg-arg-nhmec; (ii) a 54-kda proteinase which was most active on z-phe-arg-nhm ... | 1990 | 2320056 |
| [the effect of nitrofuran and asymmetrically substituted urea on trichomonas vaginalis and tritrichomonas foetus in vitro and in vivo]. | antitrichomonal activity of selected nitrofuran derivatives and asymmetrically substituted ureas was investigated in vitro and in vivo and compared with the activity of the effective therapeutic agent tinidazole. in experimental infection with trichomonas vaginalis and tritrichomonas foetus in mice, some substituted urea derivatives exhibited an antiparasitic effect slightly lower than that of tinidazole. the substance n1-(3-nitrophenyl)-n3-(2-pyrimidinyl)urea proved to be the most effective of ... | 1990 | 2322865 |
| fatty acid and sterol metabolism of cultured trichomonas vaginalis and tritrichomonas foetus. | trichomonas vaginalis and tritrichomonas foetus grown in a fetal calf serum-based culture medium, contained as major lipids (i.e., greater than 10% of total) cholesterol, phosphatidylethanolamine and phosphatidylcholine. t. vaginalis also contained sphingomyelin and t. foetus glycophosphosphingolipids. the culture medium contained (greater than 10%) cholesterol, phosphatidylethanolamine, phosphatidylcholine, sphingomyelin and lysophosphatidylcholine. the fatty acyl groups of these major lipids o ... | 1990 | 2325705 |
| the role and prevalence of gardnerella vaginalis in anaerobic vaginosis. | 310 cases of anaerobic vaginosis and 80 asymptomatic females were studied for the detection of various organisms from their genital specimens. the main complaint of the symptomatic cases was vaginal discharge. neisseria gonorrhoeae, candida albicans, trichomonas vaginalis. bacteroides species, anaerobic cocci and gardnerella vaginalis were detected. the frequency of detection of all except c. albicans was less in pregnant than non-pregnant women. however, the prevalence of anaerobes and g. vagin ... | 1990 | 2332249 |
| in vitro study on generation time (g) of trichomonas vaginalis. | 31 strains of t. vaginalis were isolated from female patients attending venereal diseases clinic, middlesex hospital, london. cloned organisms were studied in vitro for generation time (g). according to generation time t. vaginalis strains were divided into three different groups: slow, medium and fast grow. | 1990 | 2332655 |
| microbiological patterns of four new imidazole derivatives. | the authors present an in vitro experimental study concerning the microbiological properties of four imidazole derivatives, with a piperazine group between two benzene rings. seventy strains of gram-positive and gram-negative bacteria, 30 of yeasts, 14 of filamentous fungi and 10 strains of trichomonas vaginalis were tested. the new compounds revealed a good inhibitory activity against gram-positive bacteria, yeasts and dermatophytes. these results compare well with those obtained with the other ... | 1990 | 2332783 |
| growth of trichomonas vaginalis in commercial culture media. | there are only two commercially available, ready-to-use culture media which are approved by the food and drug administration for clinical diagnosis of vaginal trichomoniasis: kupferberg's sts and diamond's medium (modified). diamond's medium (klaas modification), recommended by the centers for disease control for the isolation of trichomonas vaginalis, was compared in vitro to kupferberg's (sts) medium. growth studies using six fresh clinical isolates, all from different patients, showed that wh ... | 1990 | 2351739 |
| specific erythrocyte binding is an additional nutrient acquisition system for trichomonas vaginalis. | specific receptor-mediated binding by trichomonas vaginalis of human erythrocytes was demonstrated. the ability of live parasites to internalize erythrocytes was also documented. in vitro growth assays during lipid-free and iron-limiting conditions that do not support the survival of t. vaginalis organisms showed that purified erythrocyte lipids and hemoglobin were each able to provide lipids and/or hemoglobin iron for trichomonal growth and multiplication. parasites bound hemoglobin in a highly ... | 1990 | 2351937 |
| difference in effect of dog seminal fluid and human seminal fluid and semen on in vitro survival of trichomonas vaginalis. | the authors examined the ability of dog seminal fluid to serve as an animal model in order to study survival of trichomonas vaginalis in human urogenital secretions. two strains were used: svi-1, a recent isolate from a male patient, and atcc 30001, a strain that has been cultured for many years. the authors incubated the t. vaginalis strains in dog seminal fluid, human seminal fluid, and human semen, at 37 degrees c for 6, 12, and 24 hrs. for both strains, survival in dog seminal fluid was much ... | 1990 | 2360129 |
| [further observation on the ultrastructural characteristics of trichomonas vaginalis]. | in longitudinal sections, t. vaginalis visualized by tem was round or ovoid with an ovoid nucleus situated close to the anterior one-third of the body. there was an independent and well-developed golgi complex dorsal to the nucleus and parabasal filaments. the anterior end of the cell had a blepharoplast complex which consisted of three parts, i.e., periflagellar canal, c-shaped structure of the pelta in the wall of periflagellar canal and five blepharoplasts. four anterior flagella and one post ... | 1990 | 2364506 |
| [the detection of trichomonas vaginalis in the rectum of patients with urogenital trichomoniasis]. | inoculations of scrapings of the rectal mucosa from 53 women with various urogenital diseases and urogenital trichomoniasis yielded trichomonas growth in johnson-trassel's medium in 8 cases. vaginal and rectal cultures of 6 patients were examined by scanning and transmission electron microscopy. vaginal trichomonas were detected in rectal and vaginal cultures of 6 patients. intestinal trichomonas were identified in rectal isolates from 5 patients. | 1990 | 2368486 |
| properties of trichomonas vaginalis grown under chemostat controlled growth conditions. | trichomonas vaginalis isolates nyh 286 and ir 78 were grown in continuous flow culture conditions in a complex trypticase-yeast extract-maltose medium supplemented with heat-inactivated horse serum. parasites could be stably maintained in the chemostat at high densities ranging from 1 x 10(6) to 1 x 10(7) organisms ml-1. growth densities, acid production, and profiles of total versus secreted trichomonad proteins were characterised at different rates of growth and ph. growth rate influenced the ... | 1990 | 2370063 |
| semen characteristics of asymptomatic males affected by trichomonas vaginalis. | trichomonas vaginalis is a sexually transmitted anaerobic parasite which causes vaginitis and cervicitis. the present study was carried out to determine the incidence of trichomonas infection in semen samples of asymptomatic men and also to understand the changes of semen characteristics in the infected individuals before treatment and after treatment with metronidazole (flagyl, 400 mg x 3x a day for 10 days). the semen obtained from 1131 men was examined for different characteristics and tricho ... | 1990 | 2380623 |
| rapid eradication of trichomonads by single-dosed nimorazole. | previous in vitro studies demonstrated the rapidity of trichomonacidal action of nimorazole (naxogin 500) which was twice that of metronidazole and many times that of tinidazole. since rapid eradication of parasites can lead to a significant decrease in transmission rate, and hence, a lower prevalence of this sexually transmitted disease, a pilot study was designed to investigate the in vivo speed of action of nimorazole. twenty females with positive wet smears for trichomonas vaginalis were tre ... | 1990 | 2380642 |
| persistent vaginitis caused by metronidazole-resistant trichomonas. | persistent trichomonal vaginitis is usually considered the result of either patient noncompliance with therapy or reinfection. drug-resistant trichomoniasis may also cause continued infection despite repeated courses of conventional metronidazole therapy. we report two cases of exceptionally persistent and prolonged infection despite repetitive and varied treatments, including parenteral metronidazole. an unconventional approach combining oral and topical therapy resulted in clinical and microbi ... | 1990 | 2381638 |
| in vivo effect of 2,4-dinitro-phenyl-substituted sugar hydrazones and phenylosazone against trichomonas vaginalis and tritrichomonas foetus. | antitrichomonal activity of selected 2,4-dinitro-phenylhydrazones and phenylosazones of sugars was investigated in vitro and in vivo and compared with the activity of tinidazole, an effective therapeutic agent. in experimental infections with trichomonas vaginalis and tritrichomonas foetus in mice glycolaldehyde-2,4-dinitro-phenylhydrazone acetate and 2,3-diacetylglycerolaldehyde-2,4-dinitro-phenylhydrazone exhibited an antiparasitic effect slightly inferior to that of tinidazole. structure-acti ... | 1990 | 2381964 |
| histopathology of endocervical infection caused by chlamydia trachomatis, herpes simplex virus, trichomonas vaginalis, and neisseria gonorrhoeae. | we determined the histologic correlates of clinically identified mucopurulent cervicitis, culture-proven cervical infection with chlamydia trachomatis, neisseria gonorrhoeae, herpes simplex virus (hsv), and vaginal infection with trichomonas vaginalis by examining cervical biopsies from 83 women. clinical mucopurulent cervicitis and culture-documented infection with one or more of these pathogens correlated histologically with intraepithelial neutrophils, reactive endocervical cells, edema, lumi ... | 1990 | 2387574 |
| trichomycin a, a heptaene macrolide: isolation and characterization. | | 1990 | 2387783 |
| oestrogen binding by and effect of oestrogen on trichomonads and bacteria. | it has been shown previously that trichomonas vaginalis, a common protozoan pathogen of the female genito-urinary tract, has binding sites for oestrogen and that its growth, adherence and chemotaxis are altered after exposure to physiological concentrations of oestrogens. two other species of trichomonad showed no physiological response to oestrogens but did have high affinity oestrogen binding with low binding capacity. to determine whether oestrogen binding sites occur in other pathogenic micr ... | 1990 | 2391707 |
| the limited value of symptoms and signs in the diagnosis of vaginal infections. | the etiology of vaginitis can be difficult to prove. to determine the relationship between clinical criteria (symptoms and signs) and three causes of vaginitis, we prospectively evaluated 22 criteria in 123 unselected symptomatic patients. diagnoses of candida albicans and trichomonas vaginalis infection were based on culture. bacterial vaginosis was defined by the presence of 3 of 4 clinical criteria. only 49% of our patients received diagnoses, and itching was the only symptom more frequently ... | 1990 | 2393324 |
| povidone-iodine in the treatment of metronidazole-resistant trichomonas vaginalis. | | 1990 | 2400364 |
| [the detection of the surface antigens of trichomonas vaginalis by using the scanning electron microscope]. | the method for the detection of surface antigens, based on antibody aggregation phenomenon, was used in scanning microscopy of t. vaginalis. after trichomonas treatment with immune blood serum diluted 1:5000--1:10,000 aggregates were detected, consisting of subunits 30-80 nm in diameter, localized on the protozoal cilia, projections, and, less frequently, body. analysis of the results following the material treatment with colloid gold-conjugated anti-igg permits a conclusion that the detected ag ... | 1990 | 2402936 |
| analysis of the proteinases of representative trichomonas vaginalis isolates. | isoelectric focusing and sodium dodecyl sulfate-polyacrylamide gel electrophoresis (sds-page) with acrylamide copolymerized with gelatin (substrate-sds-page) were combined to evaluate the proteinases of both long-term-grown and fresh isolates of trichomonas vaginalis. this two-dimensional substrate-sds-page resolved as many as 23 distinct proteinase activities in several isolates, and proteinases had relative molecular masses between 23 and 110 kilodaltons (kda). isoelectric points (pi) of prote ... | 1990 | 2403530 |
| heterogeneity of trichomonas vaginalis and discrimination among trichomonal isolates and subpopulations with sera of patients and experimentally infected mice. | the antibody response in trichomoniasis patients was examined with a variety of methodologies including enzyme-linked immunosorbent assays, indirect immunofluorescence, immunoblotting, and radioimmunoprecipitation-electrophoresis-autoradiography. based on enzyme-linked immunosorbent assay recognition of trichomonal isolates, sera from patients with trichomoniasis were categorized into reactive class i (ia, ib, and ic) and nonreactive class ii sera. a diminished ability to precipitate antibody-bi ... | 1985 | 2411656 |
| geographic variation among isolates of trichomonas vaginalis: demonstration of antigenic heterogeneity by using monoclonal antibodies and the indirect immunofluorescence technique. | although trichomonas vaginalis causes one of the most common sexually transmitted diseases, little is known about the antigenic variation of the parasite or about differences between strains in epidemiology or virulence. variation among isolates of t. vaginalis was investigated by using a panel of monoclonal antibodies, each reactive with different antigens, to test 88 isolates from diverse geographic areas of north america. all isolates of t. vaginalis reacted with at least one of the nine mono ... | 1985 | 2413147 |
| trichomonas vaginalis in the prostate gland. | although the prostate gland is believed to serve as a parasite reservoir in trichomoniasis in men, and clinical association of trichomonads with prostatitis is common, there has been, to our knowledge, no unequivocal demonstration of trichomonas vaginalis within the prostate gland. using established immunoperoxidase procedures, we have positively identified trichomonads in the prostatic urethra, glandular lumina, submucosa, and stroma. foci of nonspecific acute and chronic inflammation, as well ... | 1986 | 2421689 |
| further studies on the surface charge of various strains of trichomonas vaginalis and tritrichomonas foetus. | the surface charge of three strains of trichomonas vaginalis and five strains of tritrichomonas foetus was determined by direct measurement of the mean cellular electrophoretic mobility (epm) of cells suspended in solutions of different ionic strength and ph. no differences were observed in the mean epm among the two species, although significant differences among the strains exist. strains that are more pathogenic to mouse, as measured using the subcutaneous assay, had a surface more negative. ... | 1986 | 2425974 |
| phenotypic variation and diversity among trichomonas vaginalis isolates and correlation of phenotype with trichomonal virulence determinants. | the extent and nature of heterogeneity among representative trichomonas vaginalis isolates were evaluated by flow cytofluorometry analysis. monoclonal antibody and monospecific antiserum to an immunodominant trichomonad surface glycoprotein with a molecular weight of 267,000 (267k glycoprotein) were used to evaluate fresh isolates (jhh and ru375) and long-term grown isolates (nyh286, ir78, and jh31a) of t. vaginalis. isolates nyh286, jh31a, and jhh were made up of heterogeneous staining (positiv ... | 1986 | 2426199 |
| detection of microtubules of the flagellate trichomonas vaginalis by monoclonal antibodies specific for beta-tubulin. | monoclonal antibodies specific for mammalian beta-tubulin recognized the microtubule cytoskeleton of the flagellated protozoon trichomonas vaginalis. of seven antibodies, two demonstrated the axostyle, costa, recurrent flagellum, and anterior flagella by indirect immunofluorescence microscopy. the remaining five stained a hazy reticular pattern in the cytoplasm of formaldehydefixed, detergent-extracted organisms. western immunoblots of whole t. vaginalis extracts treated with protease inhibitors ... | 1986 | 2432269 |
| phenotypes and protein-epitope phenotypic variation among fresh isolates of trichomonas vaginalis. | fresh isolates of trichomonas vaginalis were examined for reactions to a panel of five monoclonal antibodies (mabs). four mabs (c20a3, dm126, dm116, and c55) were to distinct surface immunogens and one mab (l64) was to a cytoplasmic component. the fresh isolates were evaluated by indirect immunofluorescence (if), immunoblotting, and radioimmunoprecipitation. if assay with c20a3 mab gave isolates which were homogeneous nonstaining (negative [neg] phenotype) and isolates which were heterogeneous s ... | 1987 | 2437029 |