| transformation and toxicity evaluation of tetracycline in humic acid solution by laccase coupled with 1-hydroxybenzotriazole. | enzyme-based catalyzed oxidative coupling reactions (e-cocrs) are considered as viable technologies to transform a variety of pharmaceutical antibiotics. this study indicated that the extracellular fungal laccase from pleurotus ostreatus was effective in transforming tetracycline (tc) with 1-hydroxybenzotriazole (hbt) present at varying conditions during e-cocrs. the presence of humic acid (ha) showed suppressive effect on the transformation rate constants (k) of tc, and the k values for tc decr ... | 2017 | 28273567 |
| antimicrobial coumarins from the oyster culinary-medicinal mushroom, pleurotus ostreatus (agaricomycetes), from kenya. | pleurotus ostreatus has been widely used as food because of its nutritional and medicinal properties. these have been attributed to the presence of macronutrients, minerals, vitamins, and amino acids, among other secondary metabolites. there are, however, few reports on the antimicrobial activities of different classes of purified compounds from p. ostreatus. this led to the current study, the objective of which was to chemically characterize the antibiotic activities of p. ostreatus against sel ... | 2016 | 27910758 |
| comparative cold shock expression and characterization of fungal dye-decolorizing peroxidases. | dye-decolorizing peroxidases (dyps) from auricularia auricula-judae, bjerkandera adusta, pleurotus ostreatus and marasmius scorodonius (basidiomycota) were expressed in escherichia coli using the cold shock-inducible expression system pcold i dna. functional expression was achieved without the addition of hemin or the co-expression of any chaperones. the presence or absence of the native signal sequence had a strong impact on the success of the expression, but the effect was not consistent for t ... | 2016 | 27106285 |
| assessment of bacterial and fungal (hemi)cellulose-degrading enzymes in saccharification of ammonia fibre expansion-pretreated arundo donax. | this study reports enzymatic hydrolysis of the biomass of the giant reed (arundo donax l.) after ammonia fibre expansion (afex) pretreatment. in particular, the capacity of the arabinofuranosidase from the fungus pleurotus ostreatus recombinantly expressed in pichia pastoris rpoabf, its evolved mutant rpoabf f435y/y446f and the endo-cellulase from streptomyces sp. g12 celstrep recombinantly expressed in escherichia coli to enhance the hydrolysis of afex-treated a. donax was investigated, using t ... | 2016 | 26521250 |
| the effect of pleurotus ostreatus arabinofuranosidase and its evolved variant in lignocellulosic biomasses conversion. | the fungal arabinofuranosidase from pleurotus ostreatus poabf recombinantly expressed in pichia pastoris rpoabf and its evolved variant rpoabf f435y/y446f were tested for their effectiveness to enhance the enzymatic saccharification of three lignocellulosic biomasses, namely arundo donax, corn cobs and brewer's spent grains (bsg), after chemical or chemical-physical pretreatment. all the raw materials were subjected to an alkaline pretreatment by soaking in aqueous ammonia solution whilst the bi ... | 2014 | 25046861 |
| the inhibition of human tumor cell proliferation by rnase pol, a member of the rnase t1 family, from pleurotus ostreatus. | rnase po1 is a guanylic acid-specific ribonuclease (a rnase t1 family rnase) from pleurotus ostreatus. we determined the cdna sequence encoding rnase po1 and expressed rnase po1 in escherichia coli. a comparison of the enzymatic properties of rnase po1 and rnase t1 indicated that the optimum temperature for rnase po1 activity was 20 °c higher than that for rnase t1. an mtt assay indicated that rnase po1 inhibits the proliferation of human neuroblastoma cells (imr-32 and sk-n-sh) and human leukem ... | 2013 | 23832341 |
| expression of hygromycin b resistance in oyster culinary-medicinal mushroom, pleurotus ostreatus (jacq.:fr.)p. kumm. (higher basidiomycetes) using three gene expression systems. | three hygromycin b phosphotransferase (hph) gene expression systems for culinary-medicinal oyster mushroom, pleurotus ostreatus, plasmid pshc, pan7-1, and pbht1 were evaluated through peg/cacl(2)-mediated protoplast transformation. plasmid pshc is a newly constructed hph gene expression system, composed of escherichia coli hph gene, the p. ostreatus sdi promoter, and the camv35s terminator. the vector pan7-1 was commonly used for integrative transformation in filamentous fungi. plasmid pbhtl is ... | 2012 | 22339704 |
| efficacy of fungal decolorization of a mixture of dyes belonging to different classes. | dyes are the most difficult constituents to remove by conventional biological wastewater treatment. colored wastewater is mainly eliminated by physical and chemical procedures, which are very expensive and have drawbacks. therefore, the advantage of using biological processes, such as the biotransformation of dyes, is that they may lead to complete mineralization or formation of less toxic products. to prove the possibility of using fungal processes for decolorization and other applications, the ... | 2015 | 26273256 |
| effectiveness of dyes removal by mixed fungal cultures and toxicity of their metabolites. | decolorization of brilliant green (0.06 g/l), evans blue (0.15 g/l), and their mixture (total concentration 0.08 g/l, proportion 1:1 w/w) by fungi was studied. fungal strains [pleurotus ostreatus (bwph), gloeophyllum odoratum (dca), and fusarium oxysporum (g1)] were used separately and as a mixture of them. zootoxicity (daphnia magna) and phytotoxicity (lemna minor) changes were estimated after the end of experiment. mixtures of fungal strains were less effective in decolorization process than t ... | 2013 | 23687394 |
| molecular characterization of hetrv8-ir1, a partitivirus of the invasive conifer pathogenic fungus heterobasidion irregulare. | the heterobasidion annosum (fr.) bref. complex includes some of the most destructive conifer pathogenic fungi in the boreal forest region. h. irregulare, formerly known as the north american pine type of h. annosum, was introduced from north america into italy during the second world war and occurs as an invasive pathogen in pinus pinea stands together with the native european species h. annosum sensu stricto. we describe the complete nucleotide sequence of a new putative partitivirus from an it ... | 2013 | 23456423 |
| mushroom lectin enhanced immunogenicity of hbv dna vaccine in c57bl/6 and hbsag-transgenic mice. | dna vaccination is a promising strategy for activating immune responses against hepatitis b virus (hbv) infection. however, the accumulated data have shown that dna vaccination alone generates weak immune responses. to enhance the immunogenicity of hbv dna vaccine, lectin purified from pleurotus ostreatus (pol) was used as adjuvant of hbv dna vaccine for c57bl/6 and hbv surface antigen transgenic (hbvsag-tg) mice. our data demonstrate that low dose of pol (1 μg/mouse) in conjunction with hbv dna ... | 2013 | 23499522 |
| [protective activity of aqueous extracts from higher mushrooms against herpes simplex virus type-2 on albino mice model]. | toxicity and antiviral activity of aqueous extracts from higher mushrooms such as lentinula edodes (berk.) pegler (shiitake), pleurotus ostreatus (jacq.) p. kumm. (oyster), inonotus obliquus (ach. ex pers.) pilát (chaga), hydnellum compactum (pers.) p. karst. (compact tooth) were studied. in doses of 0.8 to 4.0 mg (dry weight) per mouse administered orally or intraperitoneally the extracts showed no acute toxicity. when the dose of the chaga extract was increased to 20 mg per mouse, a half of th ... | 2013 | 24738237 |
| comparative assessment of fungal augmentation treatments of a fine-textured and historically oil-contaminated soil. | the removal of aged hydrophobic contaminants from fine-textured soils is a challenging issue in remediation. the objective of this study was to compare the efficacy of augmentation treatments to that of biostimulation in terms of total aliphatic hydrocarbon (tah) and toxicity removal from a historically contaminated clay soil and to assess their impact on the resident microbial community. to this aim, pleurotus ostreatus, botryosphaeria rhodina and a combination of both were used as the inoculan ... | 2016 | 27220102 |
| production of cellobionate from cellulose using an engineered neurospora crassa strain with laccase and redox mediator addition. | we report a novel production process for cellobionic acid from cellulose using an engineered fungal strain with the exogenous addition of laccase and a redox mediator. a previously engineered strain of neurospora crassa (f5∆ace-1∆cre-1∆ndvb) was shown to produce cellobionate directly from cellulose without the addition of exogenous cellulases. specifically, n. crassa produces cellulases, which hydrolyze cellulose to cellobiose, and cellobiose dehydrogenase (cdh), which oxidizes cellobiose to cel ... | 2015 | 25849253 |
| chemical composition and nutritional and medicinal value of fruit bodies and submerged cultured mycelia of culinary-medicinal higher basidiomycetes mushrooms. | this research gives the results of a proximate analysis (moisture, ash, crude protein, fat, total carbohydrates, and total energy); a bioactive compounds analysis (γ-aminobutyric acid [gaba], ergothioneine, lovastatin, and cordycepin); fatty acid and amino acid analysis; and an analysis of macro- and microelement content of fruit bodies and mycelia of 15 higher basidiomycetes medicinal mushroom strains belonging to 12 species. the results obtained demonstrate that almost all investigated mushroo ... | 2014 | 24941169 |
| exploring the medicinal potential of the fruit bodies of oyster mushroom, pleurotus ostreatus (agaricomycetes), against multidrug-resistant bacterial isolates. | bacterial resistance to present-generation antibiotics is increasing drastically, which has become a major public health concern. the present study focuses on demonstrating the antimicrobial potential of fruit bodies of the culinary/medicinal oyster mushroom pleurotus ostreatus against clinical pathogens. five bacterial isolates were collected from sagar hospital in bangalore, india. the collected strains were grown on selective and differential media and antibiotic susceptibility testing was ap ... | 2016 | 27481158 |
| screening of antimicrobial, antioxidant properties and bioactive compounds of some edible mushrooms cultivated in bangladesh. | for a long time mushrooms have been playing an important role in several aspects of the human activity. recently edible mushrooms are used extensively in cooking and make part of new food in bangladesh for their beneficial properties. the aim of this study is to screen some values of mushrooms used in bangladesh. | 2015 | 25858107 |
| bioactivity of volatile organic compounds produced by pseudomonas tolaasii. | pseudomonas tolaasii is the main bacterial pathogen of several mushroom species. in this paper we report that strains of p. tolaasii produce volatile substances inducing in vitro mycelia growth inhibition of pleurotus ostreatus and p. eryngii, and agaricus bisporus and p. ostreatus basidiome tissue blocks brown discoloration. p. tolaasii strains produced the volatile ammonia but not hydrogen cyanide. among the volatiles detected by gc-ms, methanethiol, dimethyl disulfide (dmds), and 1-undecene w ... | 2015 | 26500627 |
| isolation of new pseudomonas tolaasii bacteriophages and genomic investigation of the lytic phage bf7. | sixteen lytic bacteriophages that infect pseudomonas tolaasii lmg 2342(t) were isolated from smashed sporocarps of oyster mushroom (pleurotus ostreatus) showing necrotic symptoms. on the basis of the host range investigation of the phages, they have wide infection abilities against the genus pseudomonas, mainly in the case of phages bf3, bf7, bf10, and bf15. molecular investigations have revealed that they all have dsdna genomes about 40 kbp in size. identical restriction patterns resulting from ... | 2012 | 22568623 |
| enzymatic modification of chitosan by cinnamic acids: antibacterial activity against ralstonia solanacearum. | this study aimed to identify chitosan polymers that have antibacterial activity against the bacterial wilt pathogen. the chitosan polymers were enzymatically synthesized using chitosan and five cinnamic acids (cads): caffeic acid (ca), ferulic acid (fa), cinnamic acid (cia), p-coumaric acid (coa) and chlorogenic acid (cha), using laccase from pleurotus ostreatus as a catalyst. the reaction was performed in a phosphate buffered solution under heterogenous reaction conditions. the chitosan derivat ... | 2016 | 26993531 |
| liposome-mediated mycelial transformation of filamentous fungi. | liposome-mediated transformation is common for cells with no cell wall, but has very limited usage in cells with walls, such as bacteria, fungi, and plants. in this study, we developed a procedure to introduce dna into mycelium of filamentous fungi, rhizopus nigricans lh 21 and pleurotus ostreatus td 300, by liposome-mediation but with no protoplast preparation. the dna was transformed into r. nigricans via plasmid pegfp-c1 and into p. ostreatus via 7.2 kb linear dna. the mycelia were ground in ... | 2013 | 24012298 |
| mutual interactions of pleurotus ostreatus with bacteria of activated sludge in solid-bed bioreactors. | white rot fungi are well known for their ability to degrade xenobiotics in pure cultures but few studies focus on their performance under bacterial stress in real wastewaters. this study investigated mutual interactions in co-cultures of pleurotus ostreatus and activated sludge microbes in batch reactors and different culture media. under the bacterial stress an increase in the dye decolorization efficiency (95 vs. 77.1 %) and a 2-fold elevated laccase activity (156.7 vs. 78.4 ul(-1)) were obser ... | 2016 | 27116960 |
| in vitro and in vivo antidiabetic evaluation of selected culinary-medicinal mushrooms (agaricomycetes). | management of type 2 diabetes by delaying or preventing glucose absorption using natural products is gaining significant attention. edible mushrooms are well documented for their nutritional and medicinal properties. this investigation was designed to evaluate the antidiabetic activity of aqueous extracts of selected culinary-medicinal mushrooms, namely, pleurotus ostreatus, calocybe indica, and volvariella volvacea, using in vitro models (α-amylase inhibition assay, glucose uptake by yeast cell ... | 2017 | 28322143 |
| effect of solid state fermentation medium optimization on pleurotus ostreatus laccase production. | the objective of this work was to increase laccase production by pleurotus ostreatus plab through culture medium optimization using solid state culture conditions. increased laccase activity was obtained through design of experiments (doe) using the taguchi orthogonal array (oa). seven factors, viz. lignocellulose, glucose, yeast extract, peptone, kh(2)po(4), mgso(4) 7h(2)o and mnso(4) h(2)o at three levels and ph at two levels. oa layout of l18 (2(1) x 3(7)) was selected for the proposed experi ... | 2015 | 26680722 |
| submerged cultivation of mycelium with high ergothioneine content from the culinary-medicinal golden oyster mushroom, pleurotus citrinopileatus (higher basidiomycetes). | the optimization of submerged culture of the culinary-medicinal golden oyster mushroom, pleurotus citrinopileatus, was studied using a one-factor-at-a-time, two-stage stimulation and central composite rotatable design to produce mycelia with high ergothioneine content. the optimal culture conditions for mycelia harvested at day 22 were a temperature of 25°c, an inoculation ratio of 5%, 2% glucose, 0.5% yeast extract, and adjustment of the initial ph value to 10. the biomass and ergothioneine con ... | 2015 | 26559861 |
| hydrophilic compounds in liquids of enzymatic hydrolyzed spruce and pine biomass. | organic acids are used for starting compounds in material sciences and in biorefinery, food, fuel, pharmaceutical, and medical industry. here, we provide the data from a biochemical approach made to investigate production of organic acids and isolation of metals from wood, which is the most abundant biomass. spruce and bark, phloem, and heartwood from pine were fermented with either microbes of oyster mushroom (pleurotus ostreatus), baker's yeast, or lactic acid bacteria to improve selective fer ... | 2015 | 26543881 |
| differential expression of laccase genes in pleurotus ostreatus and biochemical characterization of laccase isozymes produced in pichia pastoris. | in this study, transcriptome analysis of twelve laccase genes in pleurotus ostreatus revealed that their expression was differentially regulated at different developmental stages. lacc5 and lacc12 were specifically expressed in fruiting bodies and primordia, respectively, whereas lacc6 was expressed at all developmental stages. lacc1 and lacc3 were specific to the mycelial stage in solid medium. in order to investigate their biochemical characteristics, these laccases were heterologously express ... | 2015 | 26539044 |
| iron bioaccumulation in mycelium of pleurotus ostreatus. | pleurotus ostreatus is able to bioaccumulate several metals in its cell structures; however, there are no reports on its capacity to bioaccumulate iron. the objective of this study was to evaluate cultivation variables to increase iron bioaccumulation in p. ostreatus mycelium. a full factorial design and a central composite design were utilized to evaluate the effect of the following variables: nitrogen and carbon sources, ph and iron concentration in the solid culture medium to produce iron bio ... | 2015 | 26221108 |
| statistical optimization of laccase production and delignification of sugarcane bagasse by pleurotus ostreatus in solid-state fermentation. | laccases are oxidative enzymes related to the degradation of phenolic compounds, including lignin units, with concomitant reduction of oxygen to water. delignification is a necessary pretreatment step in the process of converting plant biomass into fermentable sugars. the objective of this work was to optimize the production of laccases and to evaluate the delignification of sugarcane bagasse by pleurotus ostreatus in solid-state fermentation. among eight variables (ph, water activity, temperatu ... | 2015 | 26180784 |
| hydrophilic compounds in liquids of enzymatic hydrolyzed spruce and pine biomass. | this study was focused on organic acids and metals in biofluids of wood. without seasoning, fresh woods from spruce and bark, phloem, and heartwood from pine were used as materials, which were degraded with either microbes of oyster mushroom, baker's yeast, or lactic acid bacteria. due to neutral ph of the fluids, ambient temperature, atmospheric pressure, and short reaction time, native wood microbe populations were supposed to be present. the water content of the fresh woods was 4 to 20%. the ... | 2015 | 26070851 |
| detoxification of 5-hydroxymethylfurfural by the pleurotus ostreatus lignolytic enzymes aryl alcohol oxidase and dehydrogenase. | current large-scale pretreatment processes for lignocellulosic biomass are generally accompanied by the formation of toxic degradation products, such as 5-hydroxymethylfurfural (hmf), which inhibit cellulolytic enzymes and fermentation by ethanol-producing yeast. overcoming these toxic effects is a key technical barrier in the biochemical conversion of plant biomass to biofuels. pleurotus ostreatus, a white-rot fungus, can efficiently degrade lignocellulose. in this study, we analyzed the abilit ... | 2015 | 25897324 |
| characterisation of potential antidiabetic-related proteins from pleurotus pulmonarius (fr.) quél. (grey oyster mushroom) by maldi-tof/tof mass spectrometry. | pleurotus pulmonarius has been reported to have a potent remedial effect on diabetic property and considered to be an alternative for type 2 diabetes mellitus treatment. this study aimed to investigate the antidiabetic properties of ammonium sulphate precipitated protein fractions from p. pulmonarius basidiocarps. preliminary results demonstrated that 30% (nh4)2so4 precipitated fraction (f30) inhibited saccharomyces cerevisiae α-glucosidase activity (24.18%), and 100% (nh4)2so4 precipitated frac ... | 2014 | 25243114 |
| medicinal and antimicrobial role of the oyster culinary-medicinal mushroom pleurotus ostreatus (higher basidiomycetes) cultivated on banana agrowastes in india. | oyster mushrooms, species of the genus pleurotus, are recognized for producing secondary metabolites with important medicinal properties. investigations were carried out to evaluate the antioxidative and antimicrobial properties of the edible mushroom pleurotus ostreatus (mtcc142) extracts cultivated on banana agrowastes. ethanolic extracts showed antimicrobial activities against gram-positive and gram-negative bacteria, and their in vitro antifungal activities against all fungi tested revealed ... | 2014 | 24941164 |
| culture media statistical optimization for biomass production of a ligninolytic fungus for future rice straw degradation. | the main objective of this study was to optimize a culture media for low scale biomass production of pleurotus spp. future applications of this optimization will be implemented for "in situ" rice straw degradation, increase soil nutrients availability, and lower residue and rice culture management costs. soil samples were taken from different points in six important rice production cities in colombia. for carbon and nitrogen source selection a factorial 4(2) design was carried out. the plackett- ... | 2013 | 24426109 |
| optimization of submerged fermentation conditions for lovastatin production by the culinary-medicinal oyster mushroom, pleurotus ostreatus (higher basidiomycetes). | statistical experimental designs were used to optimize lovastatin production by culinary-medicinal oyster mushroom pleurotus ostreatus obcc 1031 under submerged fermentation. the plackett-burman design was used to determine effective culture parameters, glucose, lactose, maltose, glycerol, peptone, yeast extract, nh4so2, nacl, thiamine, and agitation speed. statistical analyses of data from the plackett-burman design show that glucose, yeast extract, and agitation speed are significant parameter ... | 2013 | 24266373 |
| polysaccharide production by submerged and solid-state cultures from several medicinal higher basidiomycetes. | polysaccharides produced by microorganisms represent an industrially unexploited market. an important number of polysaccharides have been isolated from fungi, especially mushrooms, with many interesting biological functions, such as antitumor, hypoglycemic, and immunostimulating activities. in the search of new sources of fungal polysaccharides, the main goal of this research was to test the ability of several species of basidiomycetes, among them various edible mushrooms, to produce both extrac ... | 2013 | 23510286 |
| degradation and selective ligninolysis of wheat straw and banana stem for an efficient bioethanol production using fungal and chemical pretreatment. | lignocelluloses from agricultural, industrial, and forest residues constitute a majority of the total biomass present in the world. environmental concerns of disposal, costly pretreatment options prior to disposal, and increased need to save valuable resources have led to the development of value-added alternate technologies such as bioethanol production from lignocellulosic wastes. in the present study, biologically pretreated (with the fungus, pleurotus ostreatus hp-1) and chemically pretreate ... | 2013 | 28324332 |
| ethanol production by saccharomyces cerevisiae using lignocellulosic hydrolysate from chrysanthemum waste degradation. | ethanol production derived from saccharomyces cerevisiae fermentation of a hydrolysate from floriculture waste degradation was studied. the hydrolysate was produced from chrysanthemum (dendranthema grandiflora) waste degradation by pleurotus ostreatus and characterized to determine the presence of compounds that may inhibit fermentation. the products of hydrolysis confirmed by hplc were cellobiose, glucose, xylose and mannose. the hydrolysate was fermented by s. cerevisiae, and concentrations of ... | 2013 | 23117675 |
| a protein from pleurotus eryngii var. tuoliensis c.j. mou with strong removal activity against the natural steroid hormone, estriol: purification, characterization, and identification as a laccase. | a protein with strong removal activity against the natural estrogen estriol was purified from a culture supernatant of pleurotus eryngii var. tuoliensis c.j. mou. the protein was characterized as a laccase and had a molecular mass of 60kda on sds-page. the enzyme was most active at ph 7.0 and 50°c. the partial n-terminal amino acid sequence of the enzyme showed homology with laccases from mushrooms, such as pleurotus ostreatus, coriolus versicolor (current name: trametes versicolor), pycnoporus ... | 2012 | 23040398 |
| [cellulose acetate membrane electrophoresis cae and raman spectroscopy as a method identification of beta-glucans, used as biologically and therapeutically active biomaterials]. | the formation of ages progressively increases with normal aging, even in the absence of disease (the pathogenesis of diabetes associated vascular disorders and neurodegenerative diseases, including alzheimer's disease, parkinson's disease). however, they are formed at accelerated rates in age-related diseases. the polysaccharides might play a role in wound healing, both internally and externally, and also that they could play a role against inflammation and may lead to the production of better m ... | 2012 | 22783735 |
| characterization of laccase isoforms produced by pleurotus ostreatus in solid state fermentation of sugarcane bagasse. | laccases are oxidative enzymes linked to biological degradation of lignin. the aim of this work was to evaluate the effect of inducers and different concentrations of nitrogen on production level of total laccase activity and pattern of laccase isoforms, produced in solid state fermentation of sugarcane bagasse by a selected strain of pleurotus ostreatus. the addition of yeast extract 5 g/l, copper sulfate 150 μm and ferulic acid 2 mm provided highest enzymatic activity (167 u/g) and zymograms i ... | 2012 | 22487128 |
| anti-leukemic and immunomodulatory effects of fungal metabolites of pleurotus pulmonarius and pleurotus ostreatus on benzene-induced leukemia in wister rats. | the use of natural bioactive compounds in conventional chemotherapy is a new direction in cancer treatment that is gaining more research attention recently. bioactive polysaccharides and polysaccharide-protein complexes from some fungi (edible mushrooms) have been identified as sources of effective and non-toxic antineoplastic agents. selected oyster mushrooms (pleurotus pulmonarius and p. ostreatus being local [nigeria] and exotic strains, respectively) were cultured on a novel medium of yeast ... | 2012 | 22479280 |
| potentiation of innate immunity by β-glucans. | β-glucans have been known to exhibit antitumor activities by potentiating host immunity by an unknown mechanism. the c-type lectin dectin-1, a β-glucan receptor, is found on the macrophage and can recognize various β-glucans. previously, we demonstrated the presence of β-glucan receptor, dectin-1, on the raw 264.7 cells as well as on murine mucosal organs, such as the thymus, the lung, and the spleen. in order to investigate immunopotentiation of innate immunity by β-glucan, we stimulated a muri ... | 2010 | 23956643 |
| vegetative growth and phylogenetic relationship of commercially cultivated strains of pleurotus eryngii based on its sequence and rapd. | pleurotus eryngii, known as king oyster mushroom has been widely used for nutritional and medicinal purposes. this study was initiated to screen the suitable conditions for mycelial growth and to determine the phylogenetic relationship of the selected strains. optimal mycelial growth was observed at 30℃ and minimum mycelial growth observed at 10℃. this mushroom tolerates a broad ph range for mycelial growth, with most favorable growth observed at ph 6. results also indicated that glucose peptone ... | 2009 | 23983545 |
| antioxidant capacity and total phenolics content of the fruiting bodies and submerged cultured mycelia of sixteen higher basidiomycetes mushrooms from india. | the fruiting bodies and the submerged cultured mycelia of 16 higher basidiomycetes mushrooms- agaricus bisporus, armillaria mellea, auricularia auricula-judae, ganoderma applanatum, g. lucidum, laetiporus sulphureus, lentinus tigrinus, lycoperdon pyriforme, phellinus linteus, pleurotus ostreatus, p. sajor-caju, polyporus arcularius, russula brevipes, schizophyllum commune, sparassis crispa, and spongipellis unicolor-from different taxonomic groups were examined for their antioxidant capacity (ao ... | 2015 | 26756185 |
| some macronutrient content in mycelia and culture broth of medicinal mushrooms cultivated on amaranth flour. | the content of proteins, lipids, and amino and fatty acids was investigated in mycelium and culture broth of medicinal mushrooms cordyceps sinensis, pleurotus ostreatus, and schizophyllum commune. seven essential amino acids were present in the proteins of all mushroom samples, with aspartic (6.34%-14.29%) and glutamic (15.12%-17.51%) acids predominating in culture mycelium and glutamic acid (16.3%-19.1%) in culture broth. lipids in the mycelium of species c. sinensis, p. ostreatus, and sch. com ... | 2012 | 22577978 |
| intergeneric hybridization between pleurotus ostreatus and schizophyllum commune by peg-induced protoplast fusion. | intergeneric hybridization between pleurotus ostreatus and schizophyllum commune was studied using peg-induced fusion. the fusion of protoplasts from auxotrophic mutant strains resulted in the formation of fusion hybrids in the frequencies of 3.6 to 7.3×10(-5). most of these fusion hybrids were monokaryotic and sterile and no heterokaryosis occurred. most fusants showed a significantly higher nuclear dna content when compared to parental strains and no diploids (parent 1 genome plus parent 2 gen ... | 1996 | 24415417 |
| effects of pleurotus eryngii polysaccharides on bacterial growth, texture properties, proteolytic capacity, and angiotensin-i-converting enzyme-inhibitory activities of fermented milk. | pleurotus eryngii is one of the most favored oyster mushrooms and contains various beneficial bioactive compounds. polysaccharide extracted from p. eryngii (peps) was added as a natural-source ingredient to milk before fermentation, and the effects of additional peps on fermented milk were investigated in this study. the peps were extracted and added to reconstituted skim milk (12%, wt/vol) at 0.5, 0.25, and 0.125% (wt/vol) and fermented by a non-exopolysaccharide-producing strain, streptococcus ... | 2015 | 25747830 |
| fungi-based treatment of brewery wastewater-biomass production and nutrient reduction. | the beer-brewing process produces high amounts of nutrient-rich wastewater, and the increasing number of microbreweries worldwide has created a need for innovative solutions to deal with this waste. in the present study, fungal biomass production and the removal of organic carbon, phosphorus and nitrogen from synthetic brewery wastewater were studied. different filamentous fungi with a record of safe use were screened for growth, and trametes versicolor, pleurotus ostreatus and trichoderma harzi ... | 2017 | 28213731 |
| biodegradation of carbamazepine and clarithromycin by trichoderma harzianum and pleurotus ostreatus investigated by liquid chromatography - high-resolution tandem mass spectrometry (fticr ms-irmpd). | in this study, the capability of pharmaceutical biodegradation of fungus trichoderma harzianum was evaluated through the comparison with the well-known biodegradation capability of white-rot fungus pleurotus ostreatus. the study was performed in aqueous phase under aerobic conditions, using two of the most frequently detected drugs in water bodies: carbamazepine and clarithromycin, with concentrations commonly found in treated wastewater (4μg/l and 0.03μg/l respectively). for the first time, we ... | 2016 | 27039063 |
| hydrodynamics, fungal physiology, and morphology. | filamentous cultures, such as fungi and actinomycetes, contribute substantially to the pharmaceutical industry and to enzyme production, with an annual market of about 6 billion dollars. in mechanically stirred reactors, most frequently used in fermentation industry, microbial growth and metabolite productivity depend on complex interactions between hydrodynamics, oxygen transfer, and mycelial morphology. the dissipation of energy through mechanically stirring devices, either flasks or tanks, im ... | 2015 | 25652005 |
| in vitro antagonistic characteristics of bacilli isolates against trichoderma spp. and three species of mushrooms. | twenty isolates of bacillus species obtained from livestock manure composts and cotton-waste composts were tested for their antagonistic effects in vitro against three green mold pathogens of mushrooms (trichoderma harzianum, t. koningii, and t. viridescens). however, there exists a possibility bacillus species may have antagonistic effects against mushrooms themselves, and thus the same 20 isolates were tested in vitro against three species of mushrooms (flammulina velutipes, lentinus edodes, a ... | 2008 | 23997638 |
| interspecific interactions with trichoderma longibrachiatum induce pleurotus ostreatus defence reactions based on the production of laccase isozymes. | laccases are phenoloxidases involved in aromatic compound transformation but also in stress response towards antagonist species such as trichoderma sp. in this study intracellular isoforms of laccases produced by pleurotus ostreatus in liquid cultures with or without trichoderma longibrachiatum showed five isoforms with various intensities depending on the culture conditions suggesting a basal expression of these enzymes, which can be induced by interspecific interactions. a first attempt to ana ... | 2007 | 17609858 |
| variations of lignocellulosic activities in dual cultures of pleurotus ostreatus and trichoderma longibrachiatum on unsterilized wheat straw. | trichoderma spp., soil filamentous fungi, are antagonists that can cause great losses in mushroom production. we have investigated the influence of t. longibrachiatum on the production of lignocellulolytic enzymes by pleurotus ostreatus during its vegetative growth on a straw-based cultivation substrate that either had been sterilized, pasteurized or not heat treated. the variations in the lignocellulolytic activities and the electrophoretic patterns in single and dual cultures were used as a to ... | 2010 | 21148892 |
| [changes in the carbohydrate composition of cytosol from fungal spores in connection with environmental temperature and during storage]. | differences in the carbohydrate composition were revealed among spores of fungi belonging to zygomycetes, ascomycota, basidiomycota, and oomycota, part of the novel kingdom chromista. it was shown for the first time that phytophthora infestans contains arabitol in addition to glucose and trehalose. sucrose was detected in pleurotus ostreatus basidiospores. it was established that blakeslea trispora stylospores contain inositol. the dependence of the spore carbohydrate composition on the temperat ... | 2013 | 11008688 |
| evaluation of the cytotoxic effects of cam therapies: an in vitro study in normal kidney cell lines. | the purpose of this current study was to justify the incorporation of complementary and alternate medicine (cam) in current cancer treatments. the major drawback of anticancer drugs is their nonselective killing, which ultimately leads to attrition of normal cells. keeping this as the foundation of our study, we made an effort to compare the cytotoxicity associated with a known chemotherapeutic drug 5-fluorouracil (5-fu), with certain cam therapies previously reported to have anticancer activity ... | 2014 | 24672319 |
| cultivation of pleurotus ostreatus on weed plants. | oyster mushroom, pleurotus ostreatus (jacq.:fr.) kumm. itcc 3308 (collected from indian type culture collection, iari, new delhi, india, 110012) was grown on dry weed plants, leonotis sp, sida acuta, parthenium argentatum, ageratum conyzoides, cassia sophera, tephrosia purpurea and lantana camara. leonotis sp. was the best substrate in fruit body production of p. ostreatus when it was mixed with rice straw (1:1, wet wt/wet wt) for mushroom cultivation. the fruiting time for p. ostreatus was also ... | 2007 | 17161599 |
| comparative study of the physicochemical, compositional and functional properties of eight flours obtained from different plant materials found in colombia. | the physicochemical, compositional and functional properties of flour from green plantains (musa acuminata) of the large green plantain variety, oyster mushrooms (pleorotus ostreatus), pineapple peel (ananas comosus) of the 'apple pineapple' variety, yellow peas (pisum sativum), chickpeas (cicer arietinum), whole grain rice (oryza sativa), whole grain corn (zea mays) and whole grain white quinoa (chenopodium quinoa) were evaluated by using one-way analysis of variance, pearson correlations and p ... | 2016 | 27091150 |
| decolorization of salt-alkaline effluent with industrial reactive dyes by laccase-producing basidiomycetes strains. | the discharge of highly coloured synthetic dye effluents into rivers and lakes is harmful to the water bodies, and therefore, intensive researches have been focussed on the decolorization of wastewater by biological, physical or chemical treatments. in the present study, 12 basidiomycetes strains from the genus pleurotus, trametes, lentinus, peniophora, pycnoporus, rigidoporus, hygrocybe and psilocybe were evaluated for decolorization of the reactive dyes cibacron brilliant blue h-gr and cibacro ... | 2013 | 23350659 |
| development of bioprocess for the production of laccase by pleurotus ostreatus mtcc 1802 using evolutionary optimization technique. | for cost effective production of laccase enzyme (benzenediol: oxygen oxidoreductase) from p. ostreatus mtcc 1802 through solid sate fermentation, physico-chemical parameters such as temperature (20-35 degrees c), incubation period (9-17 days) and substrate (neem bark and wheat bran, in various ratios, w/w) were optimized first by one parameter at time approach and then obtained optimum conditions were considered as zero level in evolutionary optimization factorial design technique. at statistica ... | 2014 | 25434106 |
| biodegradation of aflatoxin b1 in contaminated rice straw by pleurotus ostreatus mtcc 142 and pleurotus ostreatus ghbbf10 in the presence of metal salts and surfactants. | aflatoxin b1 (afb1) is a highly toxic fungal metabolite having carcinogenic, mutagenic and teratogenic effects on human and animal health. accidental feeding of aflatoxin-contaminated rice straw may be detrimental for ruminant livestock and can lead to transmission of this toxin or its metabolites into the milk of dairy cattle. white-rot basidiomycetous fungus pleurotus ostreatus produces ligninolytic enzymes like laccase and manganese peroxidase (mnp). these extracellular enzymes have been repo ... | 2014 | 24770873 |
| effect of different substrates and casing materials on the growth and yield of calocybe indica. | calocybe indica, a tropical edible mushroom, is popular because it has good nutritive value and it can be cultivated commercially. the current investigation was undertaken to determine a suitable substrate and the appropriate thickness of casing materials for the cultivation of c. indica. optimum mycelial growth was observed in coconut coir substrate. primordia initiation with the different substrates and casing materials was observed between the 13th and 19th day. the maximum length of stalk wa ... | 2010 | 23956634 |
| solid-state fermentation of lignocellulosic plant residues from brassica napus by pleurotus ostreatus. | solid-state fermentation (ssf) of inedible parts of rapeseed was carried out using a white-rot fungus, pleurotus ostreatus, to degrade lignocellulosic material for mycelial-single cell protein (scp) production. this ssf system has the potential to be adapted to a controlled ecological life support system in space travel owing to the lack of storage space. the system for converting lignocellulosic material to scp by p. ostreatus is simple; it can be carried out in a compact reactor. the fungal ve ... | 1999 | 15304774 |
| nutrient digestibility, ruminal fermentation activities, serum parameters and milk production and composition of lactating goats fed diets containing rice straw treated with pleurotus ostreatus. | the study evaluated replacement of egyptian berseem clover (bc, trifolium alexandrinum) with spent rice straw (srs) of pleurotus ostreatus basidiomycete in diets of lactating baladi goats. nine lactating homo-parity baladi goats (average bw 23.8±0.4 kg) at 7 d postpartum were used in a triplicate 3×3 latin square design with 30 d experimental periods. goats were fed a basal diet containing 0 (control), 0.25 (srs25) and 0.45 (srs45) (w/w, dm basis) of srs. the control diet was berseem clover and ... | 2014 | 25049962 |
| treatment of petroleum drill cuttings using bioaugmentation and biostimulation supplemented with phytoremediation. | this study sought to compare the effectiveness of bioaugmentation and biostimulation, as well as the combination of both techniques, supplemented with phytoremediation, in the decontamination of petroleum drill cuttings. drill cuttings with relatively low concentration of total petroleum hydrocarbons (tph) and metals were mixed with soil in the ratio 5:1 and treated with three different combinations of the bioremediation options. option a entailed bioaugmentation supplemented with phytoremediati ... | 2016 | 27128694 |
| comparative study on the growth and yield of pleurotus ostreatus mushroom on different lignocellulosic by-products. | eight lignocellulosic by-products were evaluated as substrates for cultivation of the oyster mushroom, pleurotus ostreatus ( jacq. ex. fr) kummer. the yields of mushroom on the different substrates were 183.1, 151.8, 111.5, 87.8, 49.5, 23.3, 13.0 and 0.0 g for composted sawdust of triplochiton scleroxylon, rice straw, banana leaves, maize stover, corn husk, rice husk, fresh sawdust, and elephant grass, respectively. the biological efficiency (be) followed the same pattern and ranged from 61.0% f ... | 2003 | 12715251 |
| submerged cultivation of mycelium with high ergothioneine content from the culinary-medicinal king oyster mushroom pleurotus eryngii (higher basidiomycetes) and its composition. | the culinary-medicinal king oyster mushroom, pleurotus eryngii, was used to produce mycelia with high ergothioneine content using a one-factor-at-a-time method. the optimal culture conditions for mycelia harvested at day 14 were 25°c, 10% inoculation rate, 2% glucose, 0.5% yeast extract, and no adjustment to the initial ph value. with histidine or amino acid mix added, biomasses and the ergothioneine content of mycelia were higher than those of the control. the ergothioneine content of mycelia h ... | 2013 | 23557367 |
| degradation of benzo[a]pyrene by pleurotus ostreatus po-3 in the presence of defined fungal and bacterial co-cultures. | benzo[a]pyrene, a high molecular weight polycyclic aromatic hydrocarbon possesses carcinogenic, teratogenic, and mutagenic properties. the present study focuses on benzo[a]pyrene degradation by pleurotus ostreatus po-3, characterization and identification of metabolites produced and the extent of degradation in the presence of axenic culture of p. ostreatus po-3 and defined co-cultures of the basidiomycete with bacteria and non-basidiomycete fungi. thin-layer chromatography revealed that p. ostr ... | 2017 | 27874973 |
| ostreolysin, a pore-forming protein from the oyster mushroom, interacts specifically with membrane cholesterol-rich lipid domains. | ostreolysin, a 15 kda pore-forming protein from the edible oyster mushroom (pleurotus ostreatus), is lytic to membranes containing both cholesterol and sphingomyelin. its cytotoxicity to chinese hamster ovary cells correlates with their cholesterol contents and with the occurrence of ostreolysin in the cells detergent resistant membranes. moreover, ostreolysin binds to supported monolayers and efficiently permeabilizes sonicated lipid vesicles, only if cholesterol is combined with either sphingo ... | 2004 | 15388337 |
| synergic effect studies of the bi-enzymatic system laccase-peroxidase in a voltammetric biosensor for catecholamines. | several bi-enzymatic carbon paste biosensors modified with enzymes laccase from pleurotus ostreatus fungi and peroxidase from zucchini (cucurbita pepo) were constructed for evaluating the synergic effect of the two enzymes on the voltammetric biosensor response for various catecholamines. initially was investigated the effect of ph from 5.0 to 7.5, temperature from 25 to 50 degrees c, initial stirring time from 30 to 150 s, scan rate from 10 to 60 mvs(-1) and potential pulse amplitude from 10 to ... | 2003 | 18968977 |
| the effect of oyster mushroom β-1.3/1.6-d-glucan and oxytetracycline antibiotic on biometrical, haematological, biochemical, and immunological indices, and histopathological changes in common carp (cyprinus carpio l.). | the aim of the study was to evaluate the effect of micronized β-1.3/1.6-d-glucan (bg) derived from the oyster mushroom pleurotus ostreatus hiratake and tetracycline antibiotic oxytetracycline (otc) on biometrical, haematological, biochemical, and immunological indices, and histopathological changes in tissues of one- to two-year-old common carp (cyprinus carpio l.). the fish tested were divided into five experimental groups and one control. carp in the control group were fed commercial carp feed ... | 2013 | 24041844 |
| on the specificity of mushroom pleurotus ostreatus and pleurotus spodoleucus extracts. | extracts of mushrooms pleurotus ostreatus and pleurotus spodoleucus contain hemagglutinins with anti-h specificity similar to those found in cytisus sessilifolius and laburnum alpinum. | 1975 | 1169858 |
| phenolic removal in a model olive oil mill wastewater using pleurotus ostreatus in bioreactor cultures and biological evaluation of the process. | pleurotus ostreatus grown in bioreactor batch cultures in a model phenolic wastewater (diluted and sterilized olive oil mill wastewater-omw), caused significant phenolic removal. laccase, the sole ligninolytic enzyme detected in the growth environment, was produced during primary metabolic growth. the bioprocess was simulated with the aid of a mathematical model and the parameters of growth were determined. when the fungal biomass was increased in the reactor (during repeated batch experiments) ... | 2003 | 12909108 |
| can consumption of antioxidant rich mushrooms extend longevity?: antioxidant activity of pleurotus spp. and its effects on mexican fruit flies' (anastrepha ludens) longevity. | the variability of antioxidant capacity of 14 strains of the edible oyster mushroom pleurotus spp. was determined, and the effect of selected mushroom supplements on the longevity of the mexican fruit fly, anastrepha ludens, was evaluated. the antioxidant capacity of the fruiting bodies was determined by three different methods, measuring the free radical scavenging activity of methanolic extracts, the oh radical scavenging capacity, and the total phenol content. the inhibition percentage of the ... | 2015 | 26499817 |
| mass spectrometry based in vitro assay investigations on the transformation of pharmaceutical compounds by oxidative enzymes. | the ubiquitous presence of trace organic chemicals in wastewater and surface water leads to a growing demand for novel removal technologies. the use of isolated enzymes has been shown to possess the capability for a targeted application but requires a clearer mechanistic understanding. in this study, the potential of peroxidase from horseradish (hrp) and laccase from pleurotus ostreatus (lccpo) to transform selected trace organic chemicals was studied using mass spectrometry (ms)-based in vitro ... | 2017 | 28189026 |
| enzyme activities and chemical changes in wet olive cake after treatment with pleurotus ostreatus or eisenia fetida. | a laboratory experiment was conducted to evaluate the enzyme activities and chemical changes recorded in a recalcitrant phenolic-rich waste after treatment with pleurotus ostreatus or eisenia fetida. the waste used was wet olive cake (alperujo in spanish), a waste produced in huge amounts by the olive oil industry. both p. ostreatus and e. fetida were very effective in removing phenolic compounds, the initial concentration in the wet olive cake being reduced in both cases by around 90%. laccase ... | 2006 | 16453175 |
| mushroom extracts induce human colon cancer cell (colo-205) death by triggering the mitochondrial apoptosis pathway and go/g1-phase cell cycle arrest. | functional foods are extensively studied for their cancer preventive effects. in the present study, we compared the anti-cancer activity of aqueous extracts of three species of mushrooms including: pleurotus ostreatus (pae), auricularia polytricha (aae) and macrolepiota procera (mae) on colo-205 cells. | 2015 | 25959910 |
| enhanced antiproliferative effects of aqueous extracts of some medicinal mushrooms on colon cancer cells. | cancer is one of the most prevalent chronic diseases of the world. certain edible mushroom species are rich in antioxidants, which perform a vital role in preventing this risk in manifesting itself. initial screening was followed by qualitative phytochemical analysis; estimation of total phenolic content, dpph radical scavenging activity, and the ferric-reducing ability of plasma (frap) of the ethanolic and the aqueous extracts of 3 edible medicinal mushroom species, namely, auricularia polytric ... | 2013 | 23662617 |
| oyster culinary-medicinal mushroom, pleurotus ostreatus (higher basidiomycetes), growth in grain-based diet improves broiler chicken production. | many alternative compounds have been tested to improve poultry performance but few of them have previously used mycelial-colonized substrate to partially replace standard diet in broiler chickens. the objective of this study was to evaluate broiler chicken production, health, and meat sensory characteristics, with partial replacement of the standard diet by pleurotus ostreatus-colonized substrate. one hundred fifty 1-day-old male cobb chicks were given standard diet partially replaced by 0, 5, 1 ... | 2015 | 25746622 |
| assessing the effect of composting cassava peel based substrates on the yield, nutritional quality, and physical characteristics of pleurotus ostreatus (jacq. ex fr.) kummer. | cassava peel based substrate formulations as an alternative substrate were used to grow mushrooms. the effect of two compost heights, three composting periods on the mycelia growth, physical characteristics, yield, and nutritional qualities of pleurotus ostreatus (jacq. ex fr.) kummer was studied. mean mycelia growth of 16.2 cm after a period of seven (7) weeks was the best for 1.5 m compost height. cap diameter and stipe length differed significantly (p < 0.05) with the compost heights (0.8 m a ... | 2014 | 25580299 |
| clues for regulatory processes in fungal uptake and transfer of minerals to the basidiospore. | several fungal species are notorious for the preferential acquisition of toxicants such as ascdhgpbu in their wild-grown basidiomes, but it is not known how, or whether at all, mineral uptake is regulated. in this study, basidiomes of kuehneromyces mutabilis, pleurotus ostreatus, and hypholoma fasciculare were grown on fagus sylvatica logs embedded in sand, uranium-overburden soil, and garden soil (sio) at a lab scale to raise the accessible mineral resources 30 to >1,000 times over those availa ... | 2013 | 23761201 |
| exploring glycoside hydrolases and accessory proteins from wood decay fungi to enhance sugarcane bagasse saccharification. | glycoside hydrolases (ghs) and accessory proteins are key components for efficient and cost-effective enzymatic hydrolysis of polysaccharides in modern, biochemically based biorefineries. currently, commercialized ghs and accessory proteins are produced by ascomycetes. however, the role of wood decay basidiomycetes proteins in biomass saccharification has not been extensively pursued. wood decay fungi degrade polysaccharides in highly lignified tissues in natural environments, and are a promisin ... | 2016 | 27222665 |
| potential of fungal co-culturing for accelerated biodegradation of petroleum hydrocarbons in soil. | the potential of fungal co-culture of the filamentous pestalotiopsis sp. ng007 with four different basidiomycetes--trametes versicolor u97, pleurotus ostreatus pl1, cerena sp. f0607, and polyporus sp. s133--for accelerating biodegradation of petroleum hydrocarbons (phcs) was studied using three different physicochemical characteristic phcs in soil. all the combinations showed a mutual intermingling mycelial interaction on the agar plates. however, only ng007/s133 (50/50) exhibited an optimum gro ... | 2014 | 24997261 |
| efficiency of treatments for controlling trichoderma spp during spawning in cultivation of lignicolous mushrooms. | trichoderma spp is the cause of the green mold disease in mushroom cultivation production. many disinfection treatments are commonly applied to lignocellulose substrates to prevent contamination. mushroom growers are usually worried about the contaminations that may occur after these treatments during handling or spawning. the aim of this paper is to estimate the growth of the green mold trichoderma sp on lignocellulose substrates after different disinfection treatments to know which of them is ... | 2014 | 25763030 |
| box-behnken methodology for cr (vi) and leather dyes removal by an eco-friendly biosorbent: f. vesiculosus. | this study focused on leather industrial effluents treatment by biosorption using fucus vesiculosus as low-cost adsorbent. these effluents are yellowish-brown color and high concentration of cr (vi). therefore, biosorption process was optimized using response surface methodology based on box-behnken design operating with a simulated leather effluent obtained by mixture of cr (vi) solution and four leather dyes. the key variables selected were initial solution ph, biomass dosage and cacl2 concent ... | 2014 | 24484851 |
| preliminary results on antigenotoxic effects of dried mycelia of two medicinal mushrooms in drosophila melanogaster somatic mutation and recombination test. | in this study, the antigenotoxic effects of dried mycelia from trametes versicolor and pleurotus ostreatus were investigated using a drosophila melanogaster somatic mutation and recombination test (smart), which is based on the principle that the loss of heterozygosity of suitable recessive marker hairs, such as multiple wing (mwh) and flare-3 (flr3), can lead to the formation of mutant clones of larval cells, which then are expressed as spots on the wings of the adult flies. in this study, drie ... | 2013 | 23796223 |
| the mitochondrial genome of moniliophthora roreri, the frosty pod rot pathogen of cacao. | in this study, we report the sequence of the mitochondrial (mt) genome of the basidiomycete fungus moniliophthora roreri, which is the etiologic agent of frosty pod rot of cacao (theobroma cacao l.). we also compare it to the mtdna from the closely-related species moniliophthora perniciosa, which causes witches' broom disease of cacao. the 94 kb mtdna genome of m. roreri has a circular topology and codes for the typical 14 mt genes involved in oxidative phosphorylation. it also codes for both rr ... | 2012 | 22559916 |
| laccase production by the aquatic ascomycete phoma sp. uhh 5-1-03 and the white rot basidiomycete pleurotus ostreatus dsm 1833 during submerged cultivation on banana peels and enzyme applicability for the removal of endocrine-disrupting chemicals. | this work aimed to study the production of laccase from pleurotus ostreatus dsm 1833 and phoma sp. uhh 5-1-03 using banana peels as alternative carbon source, the subsequent partial purification and characterization of the enzyme, as well the applicability to degrade endocrine disruptors. the laccase stability with ph and temperature, the optimum ph, the k (m) and v(max) parameters, and the molar mass were determined. tests were conducted for assessing the ability of degradation of the endocrine ... | 2012 | 22371062 |
| selenium uptake by edible oyster mushrooms (pleurotus sp.) from selenium-hyperaccumulated wheat straw. | in an effort to produce selenium (se)-fortifying edible mushrooms, five species of oyster mushroom (pleurotus sp.), were cultivated on se-rich wheat straw collected from a seleniferous belt of punjab, india. total selenium was analyzed in the selenium hyperaccumulated wheat straw and the fruiting bodies. significantly high levels (p<0.0001) of se uptake were observed in fruiting bodies of all mushrooms grown on se-rich wheat straw. to the best of our knowledge, accumulation and quantification of ... | 2013 | 23535542 |
| extracellular enzymes of the white-rot fungus fomes fomentarius and purification of 1,4-β-glucosidase. | production of the lignocellulose-degrading enzymes endo-1,4-β-glucanase, 1,4-β-glucosidase, cellobiohydrolase, endo-1,4-β-xylanase, 1,4-β-xylosidase, mn peroxidase, and laccase was characterized in a common wood-rotting fungus fomes fomentarius, a species able to efficiently decompose dead wood, and compared to the production in eight other fungal species. the main aim of this study was to characterize the 1,4-β-glucosidase produced by f. fomentarius that was produced in high quantities in liqui ... | 2013 | 23149715 |
| optimization of substrate preparation for oyster mushroom (pleurotus ostreatus) cultivation by studying different raw materials and substrate preparation conditions (composting: phases i and ii). | in recent years, oyster mushroom (pleurotus ostreatus) has become one of the most cultivated mushrooms in the world, mainly in brazil. among many factors involved in a mushroom production, substrate preparation is the most critical step, which can be influenced by composting management techniques. looking forward to optimizing the substrate preparation process, were tested different composting conditions (7 and 14 days of composting with or without conditioning), potential raw materials (decumbe ... | 2016 | 27696289 |
| evaluation of the synergistic effect of mixed cultures of white-rot fungus pleurotus ostreatus and biosurfactant-producing bacteria on ddt biodegradation. | ddt (1,1,1-trichloro-2,2-bis(4-chlorophenyl) ethane) is one of the organic synthetic pesticides that has many negative effects for human health and the environment. the purpose of this study was to investigate the synergistic effect of mixed cutures of white-rot fungus, pleurotus ostreatus, and biosurfactant-producing bacteria, pseudomonas aeruginosa and bacillus subtilis, on ddt biodegradation. bacteria was added into p. ostreatus culture (mycelial wet weight on average by 8.53 g) in concentrat ... | 2017 | 28434213 |
| characterization of the rcsa gene from pantoea sp. strain ppe7 and its influence on extracellular polysaccharide production and virulence on pleurotus eryngii. | rcsa is a positive activator of extracellular polysaccharide (eps) synthesis in the enterobacteriaceae. the rcsa gene of the soft rot pathogen pantoea sp. strain ppe7 in pleurotus eryngii was cloned by pcr amplification, and its role in eps synthesis and virulence was investigated. the rcsa protein contains 3 highly conserved domains, and the c-terminal end of the open reading frame shared significant amino acid homology to the helix-turn-helix dna binding motif of bacterial activator proteins. ... | 2017 | 28592946 |
| photo-bio-synthesis of irregular shaped functionalized gold nanoparticles using edible mushroom pleurotus florida and its anticancer evaluation. | a green chemistry approach to the synthesis of gold nanoparticles using edible mushroom pleurotus florida (oyster mushroom) by photo-irradiation method has been attempted. the mixture containing the aqueous gold ions and the mushroom extract was exposed to sunlight; this resulted in the formation of biofunctionalized gold nanoparticles. these nanoparticles were characterized using various techniques like uv-visible spectroscopy; x-ray diffraction studies, energy dispersive x-ray analysis, field ... | 2013 | 23747539 |
| use of the yeast-like cells of tremella fuciformis as a cell factory to produce a pleurotus ostreatus hydrophobin. | to obtain hydrophobin, a class i hydrophobin gene, po.hyd from pleurotus ostreatus, was transformed into the yeast-like cells of tremella fuciformis using agrobacterium tumefaciens. | 2017 | 28470627 |
| biodegradation of pcbs by ligninolytic fungi and characterization of the degradation products. | the aim of the present study was to compare the degrading capabilities of eight ligninolytic fungal representatives towards a technical mixture of polychlorinated biphenyls (delor 103). axenic cultures of the fungi, either in complex or n-limited liquid media, were spiked with the technical mixture of delor 103. all of the fungal strains were able to degrade the pollutant significantly after 6weeks of incubation in both media. outstanding results were achieved by the treatment with pleurotus ost ... | 2012 | 22546633 |
| three new amino acid derivatives from edible mushroom pleurotus ostreatus. | three new amino acid derivatives, oxalamido-l-phenylalanine methyl ester (1), oxalamido-l-leucine methyl ester (2), and lumichrome hydrolyzate (3), together with nine known compounds (4-12), were isolated from the solid culture of edible mushroom pleurotus ostreatus. their structures were elucidated on the basis of extensive spectroscopic analysis. the absolute configurations of 1 and 2 were established by the chiral synthesis and confirmed by circular dichroism (cd) analysis of their total synt ... | 2017 | 28395537 |
| gene cloning, expression, and characterization of trehalose-6-phosphate synthase from pleurotus ostreatus. | trehalose-6-phosphate synthase (tps; ec2.4.1.15) catalyzes the first step in trehalose synthesis, which involves transfer of glucose from uridine diphosphate glucose (udpg) to glucose 6-phosphate (g6p) to form trehalose-6-phosphate. to determine the gene and enzymatic characteristics of tps in pleurotus ostreatus, we cloned and sequenced the cdna of potps1, which contains a 1665 bp open reading frame that encodes a 554-amino acid protein with a predicted molecular weight of 62.01 kda. this gene ... | 2017 | 28513878 |
| biodegradation of ciprofloxacin by white rot fungus pleurotus ostreatus. | unrestricted and reckless use of antibiotics has resulted in their accumulation in environment. this, in turn, has led to the emergence of multiple drug-resistant microbes. the present study focuses on degradation of ciprofloxacin (cip) by an edible white rot fungus pleurotus ostreatus. effect of cip was determined on radial growth and biomass of p. ostreatus. titrimetric and spectrophotometric assays were carried out to assess the degrading potential of p. ostreatus towards cip. it was found th ... | 2017 | 28452015 |
| mushroom lectin overcomes hepatitis b virus tolerance via tlr6 signaling. | currently, chronic hepatitis b virus (hbv) infection remains a serious public health problem in the world. recombinant hbv vaccine, as a preventive strategy against hbv infection, generates high antibody level, but it is not effective to activate innate and cellular immunity for chronic hbv infection therapy. lectins from mushroom are natural and active proteins which have been shown important biological functions. however, little is known about the immunological mechanism engaged by mushroom le ... | 2017 | 28724955 |