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stereo and regioselective microbial reduction of the clerodane diterpene 3,12-dioxo-15,16-epoxy-4-hydroxycleroda-13(16),14-diene.the biotransformation of the clerodane diterpene, 3,12-dioxo-15,16-epoxy-4-hydroxy-cleroda-13(16),14-diene (1), obtained from croton micans var. argyroglossum (baill.) mill., was investigated for the first time. whole cells of cunninghamella echinulata and rhizopus stolonifer were used as enzymatic systems, and with both fungi the only biotransformation product obtained was the new ent-neo-clerodane diterpene (3r,4s,5s,8s,9r,10s)-3,4-dihydroxy-15,16-epoxy-12-oxo-cleroda-13(16),14-diene (2a). the ...201425115072
effect of linker flexibility on the catalytic features of a glycoside hydrolase family 45 endoglucanase from rhizopus stolonifer.this study investigated an endoglucanase (egii) from rhizopus stolonifer var. reflexus tp-02 that consists of a c-terminal catalytic domain and an n-terminal carbohydrate-binding module joined by a linker rich in glycine, serine, threonine, and alanine. site-directed mutagenesis was applied to characterize the conformation and dynamics of the linker. mutants were expressed in escherichia coli bl21 and purified by ni-chelating column. structural analysis indicated that glycine provided flexibilit ...201526210786
expression and functional analysis of a glycoside hydrolase family 45 endoglucanase from rhizopus stolonifer.a novel endoglucanase gene was cloned from rhizopus stolonifer and expressed in escherichia coli, the gene product eg ii (45 kda) was assigned to glycoside hydrolase family 45 (gh45), and its specific activity on phosphoric acid-swollen cellulose (pasc) was 48 iu/mg. to solve the problem of substrate accumulation in the cellulose hydrolysis and enhance the catalytic efficiency of endoglucanase, the eg2 gene was modified by site directed mutagenesis. mutations generated by overlapping pcr have be ...201425164957
a streptomyces chitosanase is active in transgenic tobacco.growth inhibition towards rhizopus nigricans, fusarium oxysporum f. sp. radicis-lycopersici, verticillium albo-atrum and pythium ultimum was observed in vitro using a purified chitosanase from an actinomycete, streptomyces sp, strain n174. the corresponding gene, with its own signal peptide, was inserted into pbi121.7 shuttle vector to transform tobacco. transgenic plants were analysed for chitosanase activity by a sodium dodecyl sulfate-polyacrylamide gel electrophoresis assay. two major and on ...199524185780
purification of cytochrome p450 from filamentous fungus rhyzopus nigricans.it has been reported in our previous studies that steroid hydroxylation system of rhizopus nigricans involves cytochrome p450 and nadph-cytochrome p450 reductase in the electron transport system. both enzymes are membrane bound and are located in the microsomal preparations of progesterone induced fungal mycelia. in order to identify and characterize the cytochrome p450 component of fungal monoxygenase system, microsomal proteins from induced mycelia were subjected to high q anion exchange and m ...200028176091
comparison of two monooxygenase systems with cytochrome p450 in filamentous fungus rhizopus nigricans.besides the progesterone inducible steroid hydroxylase which catalyses the transformation of progesterone to 11α-hydroxyprogesterone the presence of another monooxygenase system in filamentous fungus rhizopus nigricans (r. nigricans) - lanosterol demethylase was confirmed. lanosterol demethylase was not inducible with progesterone. after subcellular fractionation both monooxygenase systems were found in microsomal fraction of fungus r. nigricans. to find out how to differentiate between the hydr ...200028176089
zygomycota associated with traditional meju, a fermented soybean starting material for soy sauce and soybean paste.various zygomycota species were detected during a study of the mycobiota of meju, a brick of dried fermented soybeans, used in korean cuisine. two hundred and sixty-eight strains were isolated from 98 finished meju products collected in various regions of korea from 2009 to 2011, and 96 strains were isolated from in-process meju on various farms from 2010 to 2011. the isolated zygomycota were identified using phenotypic characteristics combined with dna sequences of the internal transcribed spac ...201222752901
extracellular biosynthesis of silver nanoparticles using rhizopus stolonifer.synthesis of silver nanoparticles (agnps) has become a necessary field of applied science. biological method for synthesis of agnps by rhizopus stolonifer aqueous mycelial extract was used. the agnps were identified by uv-visible spectrometry, x-ray diffraction (xrd), transmission electron microscopy (tem) and fourier transform infrared spectrometry (ft-ir). the presence of surface plasmon band around 420 nm indicates agnps formation. the characteristic of the agnps within the face-centered cubi ...201728053592
optimization of medium for extracellular nuclease formation from rhizopus stolonifer.a strain of rhizopus stolonifer produced a high activity of extracellular dnaase when grown on ypg (yeast extract peptone glucose) medium. the source of peptone had a marked effect on the enzyme yield and only one peptone (i.e. from sarabhai m. chemicals ltd, india) supported enzyme production. maximum enzyme activity (88 u/ml) was obtained after 4 days' growth under submerged conditions in ypg medium containing 100 μm mn(2+), co(2+) or mg(2+), and glucose as the sole carbon source. the unpurifi ...199324419948
effect of apiforol and apigeninidin on growth of selected fungi.selected fungi were grown on agar plates in the presence of naringenin, apiforol, apiforol 7-o-rhamnoglucoside, or apigeninidin. of the four compounds tested, only apigeninidin inhibited the growth offusarium oxysporum, gibberella zeae, gliocladium roseum, altemaria solani, and phytophthora infestons. in contrast, the growth ofrhizoctonia solani, sclerotium rolfsii, and rhizopus stolonifer (- and +) was not effected by any compound. since apigeninidin is present in seeds ofsorghum sp., we hypoth ...199124258604
production of cellulases by rhizopus stolonifer from glucose-containing media based on the regulation of transcriptional regulator cre.carbon catabolite repression is a crucial regulation mechanism in microorganisms, but its characteristic in rhizopus is still unclear. we extracted a carbon regulation gene, cre, that encoded a carbon catabolite repressor protein (cre) from rhizopus stolonifer tp-02, and studied the regulation of cre by real-time qpcr. cre responded to glucose in a certain range, where it could significantly regulate part of the cellulase genes (eg, bg, and cbh2) without cbh1. in the comparison of the response o ...201727880962
immune-enhancing activity of extracellular polysaccharides isolated from rhizopus nigricans.extracellular polysaccharides (eps1-1) was extracted from fermentation liquor of rhizopus nigricans and evaluated its immune-enhancing activities in vitro and in vivo. results suggested that the proliferation of lymphocyte was stimulated after treated with eps1-1. moreover, the activities of macrophages were enhanced by increasing the activities of phagocytosis and acid phosphatase, the production of no and the mrna levels of il-2, tnf-α and inos. furthermore, eps1-1 could significantly boost th ...201627185145
polysaccharides from rhizopus nigricans mycelia induced apoptosis and g2/m arrest in bgc-823 cells.in this study, we reported that polysaccharides (rps) from the mycelia of liquid-cultured rhizopus nigricans inhibited the proliferation and clonogenic potential of human gastric cancer bgc-823 cells. results of acridine orange/ethidium bromide and hoechst 33258 staining showed that treated cells displayed typical morphological characteristics of apoptosis such as condensation of chromatin, nuclear pyknosis and formation of apoptotic bodies. flow cytometry analyses and colorimetric assay indicat ...201323911518
the thermostability of two kinds of recombinant ∆6-fatty acid desaturase with different n-terminal sequence lengths in low temperature.two recombinant rhizopus stolonifer ∆6-fatty acid desaturase enzymes with different-length n-termini were cloned and expressed in saccharomyces cerevisiae strain invscl: lrsd6d begins with the sequence of the n-terminal of the r. stolonifer ∆6-fatty acid desaturase native, encoding a deduced polypeptide of 459 amino acids (m-s-t-l-d-r-q-s-i-f-t-i-k-e-l-e-s-i-s-q-r-i-h-d-g-d-e-e-a-m-k-f), whereas srsd6d begins with the amino acid sequence of the predicted orf, encoding a deduced polypeptide of 43 ...201323821293
biotransformation of clerodane diterpenoids by rhizopus stolonifer and antibacterial activity of resulting metabolites.microbial transformation of clerodane lactone (1) by a plant pathogen fungus, rhizopus stolonifer, resulted in the production of metabolites 3 and 4. while incubation of clerodane methyl ester (2) by r. stolonifer yielded metabolites 5-8. the structures of the transformed products were determined by the spectroscopic techniques and compounds 4, 7 and 8 were found. the antibacterial activity of clerodane diterpenoids 1 and 2 and their metabolites 3-8 were also studied. the metabolites 3-7 showed ...201323535269
degradation of metalaxyl and folpet by filamentous fungi isolated from portuguese (alentejo) vineyard soils.degradation of xenobiotics by microbial populations is a potential method to enhance the effectiveness of ex situ or in situ bioremediation. the purpose of this study was to evaluate the impact of repeated metalaxyl and folpet treatments on soil microbial communities and to select soil fungal strains able to degrade these fungicides. results showed enhanced degradation of metalaxyl and folpet in vineyards soils submitted to repeated treatments with these fungicides. indeed, the greatest degradat ...201323430293
production, statistical optimization and application of endoglucanase from rhizopus stolonifer utilizing coffee husk.coffee cherry husk (ch) is one of the major by-products obtained from coffee processing industry and accounts to 43 ± 5.9% of cellulose. screening of fungal organism for cellulase production was carried out and the potential organism was identified as rhizopus stolonifer by internal transcribed spacer's (its)-5.8s rdna analysis. a systematic study with response surface methodology (rsm) based on ccrd was used to study the interactions among the variables such as ph (3-7), moisture (40-80%) and p ...201323223909
the mitochondrial respiratory chain of rhizopus stolonifer (ehrenb.:fr.) vuill.rhizopus stolonifer (ehrenb.:fr.) vuill mitochondria contain the complete system for oxidative phosphorylation, formed by the classical components of the electron transport chain (complexes i, ii, iii, and iv) and the f(1)f(0)-atp synthase (complex v). using the native gel electrophoresis, we have shown the existence of supramolecular associations of the respiratory complexes. the composition and stoichiometry of the oxidative phosphorylation complexes were similar to those found in other organi ...201323064442
[harmful biological agents at museum workposts].the aim of the studies was to determine the level and kind of microbiological contamination of air and surfaces in museum premises with various collection specificities. in addition, the criteria for selecting indicators of contamination with harmful biological agents at museum workposts are proposed.201222779322
growth modeling to control (in vitro) fusarium verticillioides and rhizopus stolonifer with thymol and carvacrol.the aim of this study was to evaluate the antifungal activity (in vitro) of thymol and carvacrol alone or in mixtures against fusarium verticillioides and rhizopus stolonifer, and to obtain primary growth models. minimal inhibitory concentration (mic) was evaluated with fungal radial growth with thymol or carvacrol concentrations (0-1600mg/l). mixtures were evaluated using concentrations below mic values. radial growth curves were described by the modified gompertz equation. mic values of carvac ...201728947087
antioxidant, antifungal and antiviral activities of chitosan from the larvae of housefly, musca domestica l.antioxidant activity of the chitosan from the larvae of musca domestica l. was evaluated in two different reactive oxygen species assays, and inhibitory effects against seven fungi were also tested. the results showed that the chitosan had scavenging activity for hydroxyl and superoxide radicals which were similar to that of ascorbic acid. also the chitosan exhibited excellent antifungal activity, especially in the low concentration, it could significantly inhibit the growth of rhizopus stolonif ...201226434321
time-course changes in fungal elicitor-induced lignan synthesis and expression of the relevant genes in cell cultures of linum album.linum album has been shown to accumulate anti-tumor podophyllotoxin (ptox) and its related lignans. in the present study, we examined the effects of five fungal extracts on the production of lignans in l. album cell cultures. fusarium graminearum extract induced the highest increase of ptox [140μgg(-1) dry weight (dw) of the l. album cell culture] which is seven-fold greater than the untreated control, while rhizopus stolonifer extract enhanced the accumulation of lariciresinol, instead of ptox, ...201222218086
toxic ipomeamarone accumulation in healthy parts of sweetpotato (ipomoea batatas l. lam) storage roots upon infection by rhizopus stolonifer.furanoterpenoid accumulation in response to microbial attack in rotting sweetpotatoes has long been linked to deaths and lung edema of cattle in the world. however, it is not known whether furanoterpenoid ipomeamarone accumulates in the healthy-looking parts of infected sweetpotato storage roots. this is critical for effective utilization as animal feed and assessment of the potential negative impact on human health. therefore, we first identified the fungus from infected sweetpotatoes as a rhiz ...201525418792
in vitro antimalarial studies of novel artemisinin biotransformed products and its derivatives.biotransformation of antimalarial drug artemisinin by fungi rhizopus stolonifer afforded three sesquiterpenoid derivatives. the transformed products were 1α-hydroxyartemisinin (3), 3.0%, a new compound, 10β-hydroxyartemisinin, 54.5% (4) and deoxyartemisinin (2) in 9% yield. the fungus expressed high-metabolism activity (66.5%). the chemical structures of the compounds were elucidated by 1d, 2d nmr spectrometry and mass spectral data. the major compound 10β-hydroxyartemisinin (4) was chemically c ...201425183484
effects of chitosan and oligochitosan on development and mitochondrial function of rhizopus stolonifer.the antifungal activities of chitosan and oligochitosan have been used to control postharvest decay of the fruits. the effect of chitosan and oligochitosan on mycelium growth, spore germination, and mitochondrial function of rhizopus stolonifer was evaluated in order to establish a connection between fungus development and the main organelle in charge to provide energy to the cell. the mycelium growth of r. stolonifer was significantly reduced on minimum media amended with chitosan or oligochito ...201424771597
liposome-mediated mycelial transformation of filamentous fungi.liposome-mediated transformation is common for cells with no cell wall, but has very limited usage in cells with walls, such as bacteria, fungi, and plants. in this study, we developed a procedure to introduce dna into mycelium of filamentous fungi, rhizopus nigricans lh 21 and pleurotus ostreatus td 300, by liposome-mediation but with no protoplast preparation. the dna was transformed into r. nigricans via plasmid pegfp-c1 and into p. ostreatus via 7.2 kb linear dna. the mycelia were ground in ...201324012298
screening of functional rhizopus stolonifer for alcohol fermentation and production of high quality korean traditional rice wine.different strains of mold were screened for the production of high quality korean traditional rice wine with anti-hypertension and good acceptability. we isolated 867 nuruk mold strains and selected 24 for further study based on measurement of amylase activity. among them, mold no. 17 showed high ethanol production upon fermentation with saccharomyces cerevisiae as well as anti-hypertensive properties. the no. 17 strain was therefore selected as the functional mold and later identified as rhizop ...201023956639
antifungal effects of chitosan with different molecular weights on in vitro development of rhizopus stolonifer (ehrenb.:fr.) vuill.determination of the molecular weight of three types of chitosan was carried out by hpsec-ri. the effect of low, medium and high molecular weight chitosan was evaluated on development of three isolates of rhizopus stolonifer. image analysis and electronic microscopy observations were done in spores of this fungus. germination of r. stolonifer in potato dextrose broth with chitosan was also evaluated. results pointed out that the low molecular weight chitosan was more effective for inhibition of ...200826048219
enzyme activities and substrate degradation by fungal isolates on cassava waste during solid state fermentation.the growth and bioconversion potential of selected strains growing on cassava waste substrate during solid state fermentation were assessed. rhizopus stolonifer showed the highest and the fastest utilization of starch and cellulose in the cassava waste substrate. it showed 70% starch utilization and 81% cellulose utilization within eight days. the release of reducing sugars indicating the substrate saccharification or degradation potential of the organisms reached the highest value of 406.5 mg/g ...200724015097
rhizopus soft rot on pear (pyrus serotina) caused by rhizopus stolonifer in korea.rhizopus soft rot caused by rhizopus stolonifer occurred on pears (pyrus serotina) in the jinju city agricultural products wholesale market in korea from 2004 to 2005. the infection usually started from wounds due to cracking at harvest time. the lesions started as water-soaked, rapidly softened, then gradually expanded. the mycelia grew vigorously on the surface of the fruits and formed stolons. colonies on potato dextrose agar at 25℃ were white cottony to brownish black. sporangia were globose ...200624039490
bioconversion of straw into improved fodder: fungal flora decomposing rice straw.the fungal flora decomposing rice straw were investigated all over the soil of sharkia province, east of nile delta, egypt, using the nylon net bag technique. sixty-four straw-decomposing species belonging to 30 genera were isolated by the dilution plate method in ground rice straw-czapek's agar medium at ph 6. the plates were incubated separately at 5℃, 25℃ and 45℃, respectively. twenty nine species belonging to 14 genera were isolated at 5℃. the most frequent genus was penicillium (seven speci ...200524049492
characterization of plasma membrane fraction from filamentous fungus rhizopus nigricans.in the filamentous fungus rhizopus nigricans a steroid hydroxylating multienzyme system is inducible by progesterone and by several other steroids. the biological signal carried by progesterone might be mediated by receptors, located either in the plasma membrane or inside the cell. to elucidate the first possibility, plasma membrane fraction was examined for the presence of progesterone receptors. the isolation of plasma membrane from fungal homogenate containing different other membranes is di ...200028176100
isolation, partial length sequence and expression of steroid inducible hps 70 gene from rhizopus nigricans.previous studies have shown that filamentous fungus rhizopus nigricans responds to addition of different steroids into growth medium with induction of hydroxylation system and that some steroids provoke stress response. the purpose of this study was to investigate whether those steroids provoke induction of hsp70 gene(s), well studied markers of stress response in different cells and organisms. the expression studies of fungal hsp70 gene(s) using northern blot analysis showed that fungal hsp70 m ...200028176085
g-protein coupled progesterone receptors in the plasma membrane of fungus rhizopus nigricans.we have demonstrated simultaneous existence of progesterone receptors and gtpase activity in the membranes prepared from the filamentous fungus rhizopus nigricans. the results obtained with pertussis toxin treated fungal mycelium suggest that these receptors do not couple to gi-go-proteins and play a role in the induction of steroid hydroxylating enzyme system by steroid substrates in the fungus.200028008531
new anti-inflammatory metabolites by microbial transformation of medrysone.microbial transformation of the anti-inflammatory steroid medrysone (1) was carried out for the first time with the filamentous fungi cunninghamella blakesleeana (atcc 8688a), neurospora crassa (atcc 18419), and rhizopus stolonifer (tsy 0471). the objective was to evaluate the anti-inflammatory potential of the substrate (1) and its metabolites. this yielded seven new metabolites, 14α-hydroxy-6α-methylpregn-4-ene-3,11,20-trione (2), 6β-hydroxy-6α-methylpregn-4-ene-3,11,20-trione (3), 15β-hydroxy ...201627104348
antifungal activity of the pygidial gland secretion of laemostenus punctatus (coleoptera: carabidae) against cave-dwelling micromycetes.the antifungal potential of the pygidial gland secretion of the troglophilic ground beetle laemostenus punctatus from a cave in southeastern serbia against cave-dwelling micromycetes, isolated from the same habitat, has been investigated. eleven collected samples were analyzed and 32 isolates of cave-dwelling fungi were documented. a total of 14 fungal species were identified as members of the genera aspergillus, penicillium, alternaria, cladosporium, rhizopus, trichoderma, arthrinium, aureobasi ...201728584964
activity of the pterophyllins 2 and 4 against postharvest fruit pathogenic fungi. comparison with a synthetic analog and related intermediates.the antifungal activity of pterophyllin 2, pterophyllin 4, a 5-desmethyl analog of the latter and some of their synthetic intermediates, against three postharvest phytopathogenic fungi, was evaluated. the target fungi were rhizopus stolonifer, botrytis cinerea and monilinia fructicola, which affect fruits worldwide, causing important economic losses. the tests were carried out with imazalil and carbendazim as positive controls. minimum inhibitory concentrations and minimum fungicidal concentrati ...201729288027
mixed cropping regimes promote the soil fungal community under zero tillage.fungi of yield soils represent a significant portion of the microbial biomass and reflect sensitivity to changes in the ecosystem. our hypothesis was that crops included in cropping regimes under the zero tillage system modify the structure of the soil fungi community. conventional and molecular techniques provide complementary information for the analysis of diversity of fungal species and successful information to accept our hypothesis. the composition of the fungal community varied according ...201729270767
saprotrophic soil fungi to improve phosphorus solubilisation and release: in vitro abilities of several species.modern agriculture is dependent on phosphate rock (pr), which is a nonrenewable resource. improvement of phosphorus (p) availability for crops in agricultural soils represents a key strategy to slow down the depletion of pr. the aim of this study was to identify potential p biofertilisers among saprotrophic fungal species. we tested 30 fungal strains belonging to 28 taxa (4 zygomycota and 24 ascomycota) and with different life strategies. the study showed that many saprotrophic fungi have the ab ...201829159452
carvacrol and eugenol effectively inhibit rhizopus stolonifer and control postharvest soft rot decay in peaches.this study aimed to investigate the antifungal mechanism of carvacrol and eugenol to inhibit rhizopus stolonifer and the control of postharvest soft rot decay in peaches.201829044849
synthesis, characterization and antimicrobial activities of copper derivatives of nhc-ii complexes.caffeine, 1, 3, 7-trimethylxanthine is one of the xanthine derivatives that are for the most part utilized as a part of solutions as diuretics. the cu (ii) complexes have been synthesized from the n-heterocyclic carbene ligands.201729022998
physiological effects and mode of action of zno nanoparticles against postharvest fungal contaminants.increasing concerns continue to be expressed about health hazards and environmental pollution resulting from the use of conventional fungicides for postharvest disease control. nanoparticles represent an alternative solution for postharvest disease management. the objective of this work was to assess the physiological effects and the antifungal efficiency of zno nanoparticles (zno nps) against a number of fungal contaminants. the efficacy of zno nps was qualitatively and quantitatively assessed ...201728941694
self-induction system for cellulase production by cellobiose produced from glucose in rhizopus stolonifer.cellulolytic fungi have evolved a sophisticated genetic regulatory network of cellulase synthesis to adapt to the natural environment. even in the absence of lignocellulose, it still secretes low levels of "constitutive" cellulase for standby application. however, the mechanisms of this constitutive expression remain incompletely understood. here we identified a cellobiose synthetase (cbs) from rhizopus stolonifer, which has the capacity to catalyse the synthesis of cellobiose from uridine dipho ...201728860637
characterization of α-amylase from soursop (annona muricata linn.) fruits degraded by rhizopus stolonifer.rhizopus stolonifer is a fungus and one of the most common species of the genus rhizopus. the organism has been a very important microbe used in the field of industrial microbiology. it has been used in the production of many hydrolytic and extracellular enzymes among which is the α-amylase. this enzyme has found various uses in the industry. fruit juices are important sources of nutrients and they contain several important therapeutic properties that may reduce the risk of various diseases. an ...201629023043
reduction of chemical and biological oxygen demands from oil wastes via oleaginous fungi: an attempt to convert food by products to essential fatty acids.the production of waste pollutants has become a major problem for many food and oil industries. however, oil wastes can provide alternative substrates for industry, which could help to solve environmental pollution problems. furthermore, oil wastes can be used as substrates to produce unsaturated fatty acids, which are important for health.201528959287
reveromycins a and b from streptomyces sp. 3-10: antifungal activity against plant pathogenic fungi in vitro and in a strawberry food model system.this study was conducted to determine the antifungal activity of the metabolites from streptomyces sp. 3-10, and to purify and identify the metabolites. meanwhile, the taxonomic status of strain 3-10 was re-evaluated. the cultural filtrates of strain 3-10 in potato dextrose broth were extracted with ethyl acetate. the resulting crude extract at 1 and 5 μg/ml inhibited growth of 22 species in 18 genera of plant pathogenic fungi and oomycetes, accounting for 92% of the total 24 tested species, sug ...201728421050
isolation and identification of fungi associated with spoilt fruits vended in gwagwalada market, abuja, nigeria.annual reports have shown that 20% of fruits and vegetables produced are lost to spoilage. this study was undertaken to isolate and identify fungi that are associated with spoilt fruits commonly sold in gwagwalada market, abuja, and recommend appropriate control measure.201728507410
assessing the anti-fungal efficiency of filters coated with zinc oxide nanoparticles.air filters support fungal growth, leading to generation of conidia and volatile organic compounds, causing allergies, infections and food spoilage. filters that inhibit fungi are therefore necessary. zinc oxide (zno) nanoparticles have anti-fungal properties and therefore are good candidates for inhibiting growth. two concentrations (0.012 m and 0.12 m) were used to coat two types of filters (melt-blown and needle-punched) for three different periods (0.5, 5 and 50 min). rhizopus stolonifer and ...201728572995
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