| biological role of nardonella endosymbiont in its weevil host. | weevils constitute the most species-rich animal group with over 60,000 described species, many of which possess specialized symbiotic organs and harbor bacterial endosymbionts. among the diverse microbial associates of weevils, nardonella spp. represent the most ancient and widespread endosymbiont lineage, having co-speciated with the host weevils for over 125 million years. thus far, however, no empirical work on the role of nardonella for weevil biology has been reported. here we investigated ... | 2010 | 20957033 |
| flufenerim, a novel insecticide acting on diverse insect pests: biological mode of action and biochemical aspects. | a new chemical compound was tested for its insecticidal activity against several major insect pests. the compound, called "flufenerim", has a core pyrimidine structure and an unknown mode of action and showed potent activity against the sweet potato whitefly bemisia tabaci (gennadius), the green peach aphid myzus persicae (sulzer), and the african cotton leafworm spodoptera littoralis (boisduval); however, it did not show any activity against two thrips species: western flower thrips frankliniel ... | 2010 | 20958045 |
| a sweet potato gene index established by de novo assembly of pyrosequencing and sanger sequences and mining for gene-based microsatellite markers. | sweetpotato (ipomoea batatas (l.) lam.), a hexaploid outcrossing crop, is an important staple and food security crop in developing countries in africa and asia. the availability of genomic resources for sweetpotato is in striking contrast to its importance for human nutrition. previously existing sequence data were restricted to around 22,000 expressed sequence tag (est) sequences and ~ 1,500 genbank sequences. we have used 454 pyrosequencing to augment the available gene sequence information to ... | 2010 | 20977749 |
| structural characterization and hypoglycemic effects of arabinogalactan-protein from the tuberous cortex of the white-skinned sweet potato (ipomoea batatas l.). | an arabinogalactan-protein (wssp-agp) was isolated from the tuberous cortex of the white-skinned sweet potato (wssp; ipomoea batatas l.). it consists of 95% (w/w) carbohydrate and 5% (w/w) protein with high contents of hydroxyproline, alanine, and serine. its sugar composition is α-l-rha:α-l-ara:β-d-gal:β-d-glca in a molar ratio of 1.0:4.1:7.6:1.3. its weight-average molecular weight was estimated to be 126,800 g/mol by high-performance size exclusion chromatography coupled with multiangle laser ... | 2010 | 21033712 |
| repression of mineral phosphate solubilizing phenotype in the presence of weak organic acids in plant growth promoting fluorescent pseudomonads. | two phosphate solubilizing bacteria (psb), m3 and sp1, were obtained from the rhizosphere of mungbean and sweet potato, respectively and identified as strains of pseudomonas aeruginosa. their rock phosphate (rp) solubilizing abilities were found to be due to secretion high amount of gluconic acid. in the presence of malate and succinate, individually and as mixture, the p solubilizing ability of both the strains was considerably reduced. this was correlated with a nearly 80% decrease in the acti ... | 2010 | 21050746 |
| ipomoea batatas attenuates amyloid β peptide-induced neurotoxicity in icr mice. | in this study, the protective effects of 17 korean native plants against amyloid β peptide (aβ)-induced oxidative stress were screened using the 2',7'-dichlorofluorescin diacetate assay and the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. ipomoea batatas exerted the highest protective effects against oxidative stress and was selected for further investigation. to confirm the protective activity of this extract, the i. batatas extract was fed to icr mice that had been injec ... | 2010 | 21142943 |
| [consumption to feed of resident adult population in rural area of the city of ibatiba (es, brazil)]. | it is a transverse study where a questionnaire of alimentary frequency was applied (qaf) in 150 adults resident of the rural area of the city of ibatiba (es, brazil). qaf classified the alimentary consumption as: habitual (>4 times in the week), not habitual (<4 times in the week) and rarely (1 time a month), with objective of correlating the alimentary consumption with the chronic-degenerative diseases. the results evidenced a habitual consumption of rice, breads, stalk, bean, cow milk, animal ... | 2011 | 21180826 |
| nutrigenomic analysis of the protective effects of bilberry anthocyanin-rich extract in apo e-deficient mice. | intake of anthocyanin-rich foods has been associated with a reduced risk of cardiovascular diseases. supplementation with anthocyanin-rich extracts from black rice or purple sweet potato was reported to attenuate atherosclerotic lesion development in apolipoprotein e-deficient (apo e(-/-)) mice. however, the mechanism(s) of their preventive action are not completely understood. previous studies revealed that anthocyanins altered mrna levels of genes related to atherosclerosis in cultured macroph ... | 2010 | 21189870 |
| ascorbic acid, β-carotene, sugars, phenols, and heavy metals in sweet potatoes grown in soil fertilized with municipal sewage sludge. | municipal sewage sludge (mss) used for land farming typically contains heavy metals that might impact crop quality and human health. a completely randomized experimental design with three treatments (six replicates each) was used to monitor the impact of mixing native soil with mss or yard waste (yw) mixed with mss (yw +mss) on: i) sweet potato yield and quality; ii) concentration of seven heavy metals (cd, cr, mo, cu, zn, pb, and ni) in sweet potato plant parts (edible roots, leaves, stem, and ... | 2011 | 21207309 |
| sequence characterization of a peruvian isolate of sweet potato chlorotic stunt virus: further variability and a model for p22 acquisition. | sweet potato chlorotic stunt virus (spcsv) is probably the most important virus infecting sweetpotato worldwide, causing severe synergistic disease complexes with several co-infecting viruses. to date only one isolate (ug), corresponding to the ea strain has been completely sequenced. it was later shown to be unusual in that, in contrast to most isolates, it encoded an additional p22 protein at the 3' end of rna1. we report the complete sequence and genome organization of a peruvian isolate of s ... | 2011 | 21262288 |
| application of simultaneous saccharification and fermentation (ssf) from viscosity reducing of raw sweet potato for bioethanol production at laboratory, pilot and industrial scales. | the aim of this work was to research a bioprocess for bioethanol production from raw sweet potato by saccharomyces cerevisiae at laboratory, pilot and industrial scales. the fermentation mode, inoculum size and pressure from different gases were determined in laboratory. the maximum ethanol concentration, average ethanol productivity rate and yield of ethanol after fermentation in laboratory scale (128.51 g/l, 4.76 g/l/h and 91.4%) were satisfactory with small decrease at pilot scale (109.06 g/l ... | 2011 | 21277777 |
| inhibition of proliferation by agricultural plant extracts in seven human adult t-cell leukaemia (atl)-related cell lines. | adult t-cell leukaemia (atl) is caused by human t-cell leukaemia virus type i (htlv-i) infection and is resistant to conventional chemotherapy. we evaluated the inhibitory effects of agricultural plants on the proliferation of seven atl-related human leukaemia cells, using three atl cell lines (ed, su9t01 and s1t), two human t-cell lines transformed by htlv-i infection (hut-102 and mt-2) and two htlv-i-negative human t-cell acute lymphoblastic leukaemia cell lines (jurkat and molt-4). a total of ... | 2011 | 21293936 |
| genetic diversity of sweet potato begomoviruses in the united states and identification of a natural recombinant between sweet potato leaf curl virus and sweet potato leaf curl georgia virus. | in the united states, two sweet potato begomoviruses, sweet potato leaf curl virus (splcv) and sweet potato leaf curl georgia virus (splcgv), were previously identified in louisiana. in recent years, at least seven additional sweet potato begomoviruses have been identified in other parts of the world. in an effort to determine the genetic diversity and distribution of sweet potato begomoviruses in the u.s., we focused our efforts on molecular characterization of field-collected begomovirus isola ... | 2011 | 21302123 |
| distinct cavemoviruses interact synergistically with sweet potato chlorotic stunt virus (genus crinivirus) in cultivated sweet potato. | two serologically unrelated sweet potato viruses causing symptoms of vein clearing in the indicator plant ipomoea setosa were isolated and their genomes have been sequenced. they are associated with symptomless infections in sweet potato but distinct vein-clearing symptoms and higher virus titres were observed when these viruses co-infected with sweet potato chlorotic stunt virus (spcsv), a virus that is distributed worldwide and is a mediator of severe virus diseases in this crop. molecular cha ... | 2011 | 21307225 |
| influence of preceding crop on wireworm (coleoptera: elateridae) abundance in the coastal plain of north carolina. | three studies were conducted to determine the effect of preceding crop on wireworm (coleoptera: elateridae) abundance in the coastal plain of north carolina. in all three studies, samples of wireworm populations were taken from the soil by using oat, avena sativa l., baits. treatments were defined by the previous year's crop and were chosen to reflect common crop rotations in the region. across all three studies, eight wireworm species were recovered from the baits: conoderus amplicollis (gyllen ... | 2010 | 21309229 |
| starch self-processing in transgenic sweet potato roots expressing a hyperthermophilic a-amylase. | sweet potato is a major crop in the southeastern united states, which requires few inputs and grows well on marginal land. it accumulates large quantities of starch in the storage roots and has been shown to give comparable or superior ethanol yields to corn per cultivated acre in the southeast. starch conversion to fermentable sugars (i.e., for ethanol production) is carried out at high temperatures and requires the action of thermostable and thermoactive amylolytic enzymes. these enzymes are a ... | 2011 | 21365786 |
| the effects of an arabinogalactan-protein from the white-skinned sweet potato (ipomoea batatas l.) on blood glucose in spontaneous diabetic mice. | we examined the effects of an arabinogalactanprotein (wssp-agp) from ipomoea batatas l. on hyperglycemia in db/db mice. an oral glucose tolerance test indicated significantly decreased plasma glucose levels by wssp-agp. additionally, an insulin tolerance test found improvement in insulin sensitivity due to treatment with wssp-agp. this suggests that amelioration of insulin resistance by wssp-agp causes to lead its hypoglycemic effects. | 2011 | 21389600 |
| hypouricemic effects of anthocyanin extracts of purple sweet potato on potassium oxonate-induced hyperuricemia in mice. | gout is a clinical syndrome in which tissue damage is induced by a chronic metabolic disorder associated with increased concentrations of uric acid in the blood. the study investigated the hypouricemic effects of anthocyanin extracts from purple sweet potato (apsp), and allopurinol, on serum uric acid levels in hyperuricemic mice. it was found that administration of a single oral dose of 100?mg/kg apsp to such animals reduced the serum uric acid concentration to 4.10?±?0.04?mg/dl, compared with ... | 2011 | 21433153 |
| effect of heat treatment on quality, thermal and pasting properties of sweet potato starch during yearlong storage. | proper postharvest handling and storage of sweet potato is an important link in the chain from producer to consumer or manufacturing industry. heat treatments have been used as a non-chemical means to modify the postharvest quality and reduce pathogen levels and disease development of a wide variety of horticultural products. the objective of this study was to investigate the effects of hot water treatment (hwt) on the quality, gelatinisation enthalpy and pasting properties of sweet potato starc ... | 2011 | 21445886 |
| homology modeling and dynamics study of aureusidin synthase-an important enzyme in aurone biosynthesis of snapdragon flower. | aurones, a class of plant flavonoids, provide bright yellow color on some important ornamental flowers, such as cosmos, coreopsis, and snapdragon (antirrhinum majus). recently, it has been elucidated that aureusidin synthase (aus), a homolog of plant polyphenol oxidase (ppo), plays a key role in the yellow coloration of snapdragon flowers. in addition, it has been shown that aus is a chalcone-specific ppo specialized for aurone biosynthesis. aus gene has been successfully demonstrated as an attr ... | 2011 | 21470561 |
| rapid spread of a bacterial symbiont in an invasive whitefly is driven by fitness benefits and female bias. | maternally inherited bacterial symbionts of arthropods are common, yet symbiont invasions of host populations have rarely been observed. here, we show that rickettsia sp. nr. bellii swept into a population of an invasive agricultural pest, the sweet potato whitefly, bemisia tabaci, in just 6 years. compared with uninfected whiteflies, rickettsia-infected whiteflies produced more offspring, had higher survival to adulthood, developed faster, and produced a higher proportion of daughters. the symb ... | 2011 | 21474763 |
| motor command for precision grip in the macaque monkey can be mediated by spinal interneurons. | in motor control, the general view still is that spinal interneurons mainly contribute to reflexes and automatic movements. the question raised here is whether spinal interneurons can mediate the cortical command for independent finger movements like precision grip between thumb and index finger in the macaque monkey, or if this function depends exclusively on a direct corticomotoneuronal pathway. this study is a follow up of a previous report (sasaki et al. 2004, j neurophysiol 92:3142-3147) in ... | 2011 | 21511706 |
| absorption of 6-o-caffeoylsophorose and its metabolites in sprague-dawley rats detected by electrochemical detector-high-performance liquid chromatography and electrospray ionization-time-of-flight-mass spectrometry methods. | absorption and metabolism of a natural compound, 6-o-caffeoylsophorose (cs) from acylated anthocyanins in a red vinegar fermented with purple sweetpotato, were clarified. the absorption of cs and conjugated cs in blood from orally administrated sprague-dawley rats at a dose of 400 mg/kg was investigated by electrochemical detection-high performance liquid chromatography. as a result, cs was successfully detected in rat plasma (auc(0-6h), 108.6 ± 8.1 nmol h/ml) and was found to be an intact absor ... | 2011 | 21534624 |
| phenoloxidase activity of intact and chemically modified functional unit rvh1-a from molluscan rapana venosa hemocyanin. | o-diphenol oxidase activities (o-dipo) of chemically modified functional unit rvh1-a of molluscan hemocyanin rapana venosa were studied using l-dopa and dopamine as substrates. with l-dopa as substrate the native fu rvh1-a did not show any o-dipo activity. therefore the native fu rvh1-a was converted to enzymatic active form, after treatment with sds, trypsin, urea and different values of ph when its o-dipo activity was studied. the highest artificial induction of o-dipo activity was observed af ... | 2011 | 21536147 |
| expressed nifh genes of endophytic bacteria detected in field-grown sweet potatoes (ipomoea batatas l.). | we examined the nitrogenase reductase (nifh) genes of endophytic diazotrophic bacteria expressed in field-grown sweet potatoes (ipomoea batatas l.) by reverse transcription (rt)-pcr. gene fragments corresponding to nifh were amplified from mrna obtained from the stems and storage roots of field-grown sweet potatoes several months after planting. sequence analysis revealed that these clones were homologous to the nifh sequences of bradyrhizobium, pelomonas, and bacillus sp. in the dna database. i ... | 2008 | 21558693 |
| ipomoea batatas and agarics blazei ameliorate diabetic disorders with therapeutic antioxidant potential in streptozotocin-induced diabetic rats. | ipomoea batatas, agaricus blazei and smallanthus sonchifolius are known to favorably influence diabetes mellitus. to clarify their antidiabetic efficacy and hypoglycemic mechanisms, we treated streptozotocin-induced diabetic rats with daily oral feeding of powdered ipomoea batatas (5 g kg(-1) d(-1)), agaricus blazei (1 g kg(-1) d(-1)) or smallanthus sonchifolius (4 g kg(-1) d(-1)) for 2 months. treatments with ipomoea batatas or agaricus blazei, but not smallanthus sonchifolius, significantly su ... | 2011 | 21562638 |
| sweet potato (ipomoea batatas l.) leaves suppressed oxidation of low density lipoprotein (ldl) in vitro and in human subjects. | sweet potato (ipomoea batatas l.) leaves are consumed as vegetables around the world, especially in southeast asia. the aim of this study was to investigate the inhibitory effect of sweet potato leaves on low-density lipoprotein oxidation in vitro and in human subjects. we compared the antioxidant activity of 8 kinds of sweet potato leaves. every sweet potato leaf had high radical scavenging activity and prolonged a lag time for starting low-density lipoprotein oxidation in vitro. we found that ... | 2011 | 21562639 |
| optimized construction of microsatellite-enriched libraries. | the construction of microsatellite-enriched libraries is an indispensable tool to search for molecular markers as complete genome sequences are still not available for the majority of species of interest. numerous protocols are available in the literature for the construction of these libraries; however, sometimes their low efficiency or lack of optimization in the protocols can restrict their efficacy. we have designed and tested various adapters and ligation methods; we also tested oligo-repea ... | 2010 | 21565050 |
| a novel monopartite begomovirus infecting sweet potato in brazil. | the complete genome sequences of two monopartite begomovirus isolates (genus begomovirus, family geminiviridae) present in a single sweet potato (ipomoea batatas) plant collected in são paulo, brazil, are presented. based on the current taxonomic criteria for the genus begomovirus, one of the isolates was shown to represent a novel species, tentatively named sweet potato leaf curl sao paulo virus (splcspv). the other isolate represented a new strain of sweet potato leaf curl virus, named sweet p ... | 2011 | 21594599 |
| protective mechanisms of anthocyanins from purple sweet potato against tert-butyl hydroperoxide-induced hepatotoxicity. | anthocyanins have been shown to exert anti-proliferative, anti-inflammatory effects and anti-carcinogenic activity. in the present work, we investigated the protective effects of anthocyanin fraction (af) from purple sweet potato on tert-butyl hydroperoxide (t-bhp)-induced hepatotoxicity in hepg2 cell line and in rat liver. the result showed that the oral pretreatment of af before t-bhp treatment significantly lowered the serum levels of the hepatic enzyme markers (alt and ast) and reduced oxida ... | 2011 | 21640154 |
| quality evaluation of functional chicken nuggets incorporated with ground carrot and mashed sweet potato. | this study was envisaged to evaluate the effect of ground raw carrot (0%, 5%, 10% and 15%) and mashed sweet potato (0%, 5%, 10% and 15%) as functional ingredients on the quality of chicken meat nuggets. the products were evaluated for physicochemical quality, proximate composition, nutritive value, sensory quality as well as color and texture profile analyses. additions of either raw carrot or mashed sweet potato represent an improvement in the nutritional value and have some beneficial effects ... | 2011 | 21652765 |
| anthocyanins isolated from the purple-fleshed sweet potato attenuate the proliferation of hepatic stellate cells by blocking the pdgf receptor. | during the process of liver fibrosis, hepatic stellate cells (hscs) play a critical role in the increased formation and reduced degradation of extracellular matrix in the liver. we investigated the anti-proliferative effects of an anthocyanin fraction (af), isolated from the purple-fleshed sweet potato, on platelet-derived growth factor (pdgf)-bb-dependent signaling pathways in hsc-t6 cells. hsc proliferation plays a pivotal role in liver fibrogenesis. the af suppressed hsc activation, including ... | 2011 | 21787688 |
| [effects of human disturbance on soil aggregates content and their organic c stability in karst regions]. | taking the primary forest land (pf), natural restoration land (nr), grazing grassland burned annually in winter (gb), and maize-sweet potato cropland (ms) in karst regions of northwest guangxi as test objects, this paper studied the soil aggregates content and their organic c stability in the four ecosystems under different human disturbance patterns. the soil water-stable aggregates (>0.25 mm) content in pf, nr, and gb accounted for more than 70%, while that in ms was only 37%. the destruction ... | 2011 | 21774320 |
| integrating crops and livestock in subtropical agricultural systems. | as the demand for livestock products increases, and is expected to continue to increase over the next few decades, especially in developing countries, smallholder mixed systems are becoming more intensive. however, with limited land and water resources and concern about the environmental impact of agricultural practices and climate change, the challenge is to find ways of increasing productivity that do not compromise household food security, but rather increase incomes equitably and sustain or ... | 2011 | 21769884 |
| constituents in purple sweet potato leaves inhibit in vitro angiogenesis with opposite effects ex vivo. | objective: the aim of this study was to investigate the in vitro effect of polyphenols in purple sweet potato leaves (pspls) on angiogenesis in human umbilical vascular endothelial cells (huvecs). the ex vivo effect was test in human serum collected from the subjects who consumed 200 g of pspl in a low polyphenol diet versus a low polyphenol diet. methods: methanolic extract from pspls and human sera from subjects were treated with huvecs and the effects of cell proliferation, migration, tube fo ... | 2011 | 21763106 |
| biofortification: a new tool to reduce micronutrient malnutrition. | the density of minerals and vitamins in food staples eaten widely by the poor may be increased either through conventional plant breeding or through the use of transgenic techniques, a process known as biofortification. | 2011 | 21717916 |
| phytochemical screening, antioxidant, and antimicrobial activities of the crude leaves' extract from ipomoea batatas (l.) lam. | ipomoea batatas (l.) lam., popularly known as sweet potato (sp), has played an important role as an energy and a phytochemical source in human nutrition and animal feeding. ethnopharmacological data show that sp leaves have been effectively used in herbal medicine to treat inflammatory and/or infectious oral diseases in brazil. the aim of this research was to evaluate the phytochemical, antioxidant, and antimicrobial activities of the crude leaves' extract of sp leaves. | 2011 | 21716926 |
| [effect of purple sweet potato flavonids on metabolism of glucose and lipids in diabetic rats]. | to investigate the effects of purple sweet potato flavonoids (pspf) on blood glucose and lipids levels in diabetic rats. | 2011 | 21845749 |
| calf thymus dna-binding ability study of anthocyanins from purple sweet potatoes ( ipomoea batatas l.). | a total of 10 anthocyanin compounds were identified from five purple sweet potato ( ipomoea batatas l.) varieties, qunzi, zishu038, ji18, jingshu6, and ziluolan, by high-performance liquid chromatography-tandem mass spectrometry (hplc-ms/ms) to assess their calf thymus dna-binding ability in vitro. the interaction between anthocyanins and calf thymus dna in tris-hcl buffer solution (ph 6.9) was evaluated by fluorescence spectroscopy. using ethidium bromide (eb) as a fluorescence probe, fluoresce ... | 2011 | 21678894 |
| [effect of purple sweet potato on lipid metabolism and oxidative stress in hyperlipidemic rats]. | to investigate the effect of purple sweet potato on lipid metabolism and oxidative stress in hyperlipidemic rats. | 2011 | 21845747 |
| efficient embryogenic suspension culturing and rapid transformation of a range of elite genotypes of sweet potato (ipomoea batatas [l.] lam.). | efficient agrobacterium tumefaciens-mediated transformation was developed using embryogenic suspension cell cultures of elite sweet potato (ipomoea batatas [l.] lam.) cultivars, including ayamurasaki, sushu2, sushu9, sushu11, wanshu1, xushu18 and xushu22. embryogenic suspension cultures were established in lcp medium using embryogenic calli induced from apical or axillary buds on an induction medium containing 2 mg l(-1) 2,4-d. suspension cultures were co-cultivated with a. tumefaciens strain lb ... | 2011 | 21958713 |
| Purple sweet potato color attenuates domoic acid-induced cognitive deficits by promoting estrogen receptor-a-mediated mitochondrial biogenesis signaling in mice. | Recent findings suggest that endoplasmic reticulum stress may be involved in the pathogenesis of domoic acid-induced neurodegeneration. Purple sweet potato color, a class of naturally occurring anthocyanins, has beneficial health and biological effects. Recent studies have also shown that anthocyanins have estrogenic activity and can enhance estrogen receptor-a expression. In this study, we evaluated the effect of purple sweet potato color on cognitive deficits induced by hippocampal mitochondri ... | 2011 | 22178976 |
| Low genetic diversity of Squash vein yellowing virus in wild and cultivated cucurbits in the U.S. suggests a recent introduction. | Squash vein yellowing virus (SqVYV) isolates were collected from cultivated and weedy cucurbits representing major hosts and locations in the U.S. and analyzed to better understand the diversity and population structure. No differences in symptoms were observed in field-collected isolate source plants or subsequently inoculated greenhouse plants, and the complete genome of an SqVYV isolate from a wild cucurbit host (smellmelon, Cucumis melo var. dudaim) was highly similar (99.4% nucleotide ident ... | 2011 | 22142477 |
| molecular characterization of two sweepoviruses from china and evaluation of the infectivity of cloned splcv-js in nicotiana benthamiana. | sweepoviruses are important begomoviruses that infect ipomoea plants worldwide and cause sweet potato yield losses and cultivar decline. two sweepoviruses, sweet potato leaf curl virus-jiangsu (splcv-js) and sweet potato leaf curl china virus-zhejiang (splccnv-zj), were cloned from diseased sweet potato plants collected in the jiangsu and zhejiang provinces of china. sequence characterization and phylogenetic analysis demonstrated that both are typical monopartite begomoviruses and have close re ... | 2011 | 22179901 |
| Purple sweet potato anthocyanins attenuate hepatic lipid accumulation through activating adenosine monophosphate-activated protein kinase in human HepG2 cells and obese mice. | Purple sweet potato is a functional food rich in anthocyanins that possess disease-preventive properties. Anthocyanins are known to possess potent antidiabetic properties. However, the effect of the anthocyanin fraction (AF) from purple sweet potato on hepatic lipid metabolism remains unclear. Our hypothesis is that AF inhibits hepatic lipid accumulation through the activation of adenosine monophosphate-activated protein kinase (AMPK) signaling pathways in vitro and in vivo. In this study, we ev ... | 2011 | 22153515 |
| molecular cloning and functional analysis of the ortho-hydroxylases of p-coumaroyl coenzyme a/feruloyl coenzyme a involved in formation of umbelliferone and scopoletin in sweet potato, ipomoea batatas (l.) lam. | ortho-hydroxylation of cinnamates is a key step in coumarin biosynthesis in plants. ortho-hydroxylated cinnamates undergo trans/cis isomerization of the side-chain and then lactonization to form coumarins. sweet potato [ipomoea batatas (l.) lam.] accumulates umbelliferone and scopoletin after biotic and abiotic stresses. to elucidate molecular aspects of ortho-hydroxylation involved in umbelliferone formation in sweet potato, isolation and characterization of cdnas encoding 2-oxoglutarate-depend ... | 2011 | 22169019 |
| Effects of Baking and Boiling on the Nutritional and Antioxidant Properties of Sweet Potato [Ipomoea batatas (L.) Lam.] Cultivars. | The effects of baking and boiling on the nutritional and antioxidant properties of three sweet potato cultivars (Beniazuma, Koganesengan, Kotobuki) cultivated in Turkey were investigated. The samples were analyzed for proximate composition, total phenolic content, ascorbic acid, ß-carotene, antiradical activity, and free sugars. The dry matter, protein, and starch contents of the sweet potatoes were significantly changed by the treatments while the ash and crude fiber contents did not differ as ... | 2011 | 22101780 |
| polyphenol-rich sweet potato greens extract inhibits proliferation and induces apoptosis in prostate cancer cells in vitro and in vivo. | sweet potato (ipomoea batatas) leaves or greens, extensively consumed as a vegetable in africa and asia, are an excellent source of dietary polyphenols such as anthocyanins and phenolic acids. here, we show that sweet potato greens extract (spge) has the maximum polyphenol content compared with several commercial vegetables including spinach. the polyphenol-rich spge exerts significant antiproliferative activity in a panel of prostate cancer cell lines while sparing normal prostate epithelial ce ... | 2011 | 21948980 |
| [construction of a bivalent plant expression vector carrying vvsuc11 and vvsuc12 genes and its genetic transformation in sugar beet]. | we have recombined genes vvsuc11, vvsuc12 from vitis vinifera l., and root-specific promoters of sweet potato storage protein gene from ipomoea batatas l. lam., named as sp1 and sp2. we have constructed a vector pcambia2301-sp1- vvsuc11-sp2-vvsuc12 using pcambia2301 as an original vector. vvsuc11 and vvsuc12 were under the control of root-specific promoters of sweet potato storage protein gene. we transformed the vector into kws-9103 breeding line of beta vulgaris l. with agrobacterium-mediated ... | 2011 | 22097805 |
| molecular characterization of sweet potato leaf curl virus (splcv) isolates from korea: phylogenetic relationship and recombination analysis. | the complete dna genome of sweet potato leaf curl virus (splcv) from samples obtained from eight regions was amplified by pcr and characterized in this study. the dna genome of one group (splcv korea group 1) consisted of 2828 nucleotides and that of the second group (splcv korea group 2) consisted of 2829 nucleotides. sequence comparisons showed that the genome sequences of splcv korea isolates were closely related to those of splcv brazil isolates (fj969834, fj969835, and fj969836), splcv japa ... | 2011 | 22149498 |
| effect of antioxidant activity and functional properties of chingshey purple sweet potato fermented milk by lactobacillus acidophilus, l. delbrueckii subsp. lactis, and l. gasseri strains. | in this study, individual selected lactic acid bacteria strains lactobacillus acidophilus (la), l. delbrueckii subsp. lactis (ldl), and l. gasseri (lga) were grown in chingshey purple sweet potato (cpsp) substrate/media. cpsp is rich in anthocyanin, which possesses antioxidant activity and in vitro cell assay. the antioxidant ability and functional properties of the fermented milk were examined. high-performance liquid chromatographic (hplc) method was used to analyze the free amino acid, organi ... | 2011 | 22182227 |
| cloning a peanut resveratrol synthase gene and its expression in purple sweet potato. | a resveratrol synthase gene was cloned from the peanut plant (arachis hypogaea) by rt-pcr and was transformed into purple sweet potato (ipomoea batatas) by agrobacterium-mediated transformation. stem sections were infected with bacterial solution of od(600) = 0.4 for 20 min and then cocultured for 2 days. infected explants were cultured on ms media containing 50 mg/l kanamycin, 0.02 mg/l naa and 1 mg/l 6-ba for bud induction or containing 75 mg/l kanamycin, 1.0 mg/l naa and 0.1 mg/l 6-ba for roo ... | 2012 | 21932029 |
| Expression of a cloned sweet potato catalase SPCAT1 alleviates ethephon-mediated leaf senescence and H(2)O(2) elevation. | In this report a full-length cDNA, SPCAT1, was isolated from ethephon-treated mature L3 leaves of sweet potato. SPCAT1 contained 1479 nucleotides (492 amino acids) in its open reading frame, and exhibited high amino acid sequence identities (ca. 71.2-80.9%) with several plant catalases, including Arabidopsis, eggplant, grey mangrove, pea, potato, tobacco and tomato. Gene structural analysis showed that SPCAT1 encoded a catalase and contained a putative conserved internal peroxisomal targeting si ... | 2012 | 21893366 |
| Sweepoviruses cause disease in sweet potato and related Ipomoea spp.: fulfilling Koch's postulates for a divergent group in the genus begomovirus. | Sweet potato (Ipomoea batatas) and related Ipomoea species are frequently infected by monopartite begomoviruses (genus Begomovirus, family Geminiviridae), known as sweepoviruses. Unlike other geminiviruses, the genomes of sweepoviruses have been recalcitrant to rendering infectious clones to date. Thus, Koch's postulates have not been fullfilled for any of the viruses in this group. Three novel species of sweepoviruses have recently been described in Spain: Sweet potato leaf curl Lanzarote virus ... | 2011 | 22073314 |
| evidence that thaxtomin c is a pathogenicity determinant of streptomyces ipomoeae, the causative agent of streptomyces soil rot disease of sweetpotato. | streptomyces ipomoeae is the causal agent of streptomyces soil rot of sweetpotato, a disease marked by highly necrotic destruction of adventitious roots, including the development of necrotic lesions on the fleshy storage roots. streptomyces potato scab pathogens produce a phytotoxin (thaxtomin a) that appears to facilitate their entrance into host plants. s. ipomoeae produces a less-modified thaxtomin derivative (thaxtomin c) whose role in pathogenicity has not been examined. here, we cloned ... | 2011 | 22088193 |
| cloning and characterization of a sweet potato calmodulin spcam that participates in ethephon-mediated leaf senescence, h(2)o(2) elevation and senescence-associated gene expression. | in this report a full-length cdna, spcam, was isolated from ethephon-treated mature leaves of sweet potato. spcam contained 450 nucleotides (149 amino acids) in its open reading frame, and exhibited high amino acid sequence identities (ca. 76-100%) with several plant calmodulins, including arabidopsis, carrot, ghost needle weed, pea, potato, soybean, sweet chestnut, and tobacco. sweet potato spcam also contained four putative conserved calmodulin ef-hand motifs, which responded for ca(2+) bindin ... | 2012 | 22226342 |
| immunomodulatory effects of alliums and ipomoea batata extracts on lymphocytes and macrophages functions in white leghorn chickens: in vitro study. | we previously described that supplementary garlic, onion and purple sweet potato (psp) enhance humoral immune response in white leghorn chickens. in the present in vitro study, we investigated the effects of garlic (ge), onion (oe) and psp (pspe) extracts on proliferation, interleukin (il)-2 and interferon (inf)-γ gene expression of stimulated lymphocytes. the effects on microbicidal activity, reactive oxygen species (ros) and nitric oxide (no) productions of stimulated peritoneal macrophages we ... | 2012 | 22250742 |
| transgenic sweet potato expressing thionin from barley gives resistance to black rot disease caused by ceratocystis fimbriata in leaves and storage roots. | black rot of sweet potato caused by pathogenic fungus ceratocystis fimbriata severely deteriorates both growth of plants and post-harvest storage. antimicrobial peptides from various organisms have broad range activities of killing bacteria, mycobacteria, and fungi. plant thionin peptide exhibited anti-fungal activity against c. fimbriata. a gene for barley α-hordothionin (αht) was placed downstream of a strong constitutive promoter of e12ω or the promoter of a sweet potato gene for β-amylase of ... | 2012 | 22212462 |
| fastannotator--an efficient transcript annotation web tool. | recent developments in high-throughput sequencing (hts) technologies have made it feasible to sequence the complete transcriptomes of non-model organisms or metatranscriptomes from environmental samples. the challenge after generating hundreds of millions of sequences is to annotate these transcripts and classify the transcripts based on their putative functions. because many biological scientists lack the knowledge to install linux-based software packages or maintain databases used for transcri ... | 2012 | 23281853 |
| time-dose-mortality data and modeling for the entomopathogenic fungus aschersonia placenta against the whitefly bemisia tabaci. | the fungus aschersonia placenta fjsm was evaluated for control of the sweet potato whitefly, bemisia tabaci. bemisia tabaci nymphs (1st-4th instars) on tomato plants in the greenhouse (25-27 °c, 70%-85% relative humidity) were sprayed with suspensions containing 0, 10(4), 10(5), 10(6), 10(7), or 10(8) a. placenta fjsm conidia/ml. mortality of fungus-treated 1st to 3rd instar nymphs ranged from 93% to 100% but was <25% for 4th instar nymphs; the fungus sporulated from 70% to 80% of the fungus-tre ... | 2012 | 23461516 |
| a lung oedema factor from mouldy sweet potatoes (ipomoea batatas). | | 2015 | 4930474 |
| childhood malnutrition in the western province of the solomon islands. | cross-sectional anthropometric studies conducted in the western province of the solomon islands indicate that over half the children become malnourished before the age of 2 years, regardless of the nutritional indicator (weight for age, weight for height, height for age) used. in comparison to reference children, weight gain slows from the age of 6 months and mean weight is static from 9-17 months. stunting is more important than wasting. dietary research suggests that the principle cause of thi ... | 2013 | 3765099 |
| hydrolysis of aryl beta-maltotriosides by sweet potato beta-amylase and soybean beta-amylase. | sweet potato beta-amylase [ec 3.2.1.2, alpha 1,4-d-glucan maltohydrolase]-catalyzed hydrolyses of aryl beta-maltotriosides with substituents, no2-, cl-, and br- at the o-, m-, and p-positions in the phenyl ring were studied at ph 4.8 and 25 degrees c. the hydrolyses of a few of the maltotriosides by soybean beta-amylase [ec 3.2.1.2, alpha-1,4-d-glucan maltohydrolase] were also studied at ph 5.4 and 25 degrees c. it was found that the aryl beta-maltotriosides were preferentially hydrolyzed into m ... | 1998 | 147271 |
| a new method for the preparation of beta-amylase from sweet potato. | a simple method for the preparation of sweet potato beta-amylase by thymol amylose adsorption is described. the method is far more efficient and gives higher recovery of the enzyme. the crystalline enzyme thus obtained is found to be homogeneous by gel chromatography, polyacrylamide gel electrophoresis. | 2003 | 155814 |
| toxicity of novel sequiterpenoids from the stressed sweet potato (ipomoea batatas). | | 2004 | 520970 |
| separation, amino-terminal sequence and cell-free synthesis of the smallest subunit of sweet potato cytochrome c oxidase. | the smallest subunit (v) of sweet potato cytochrome c oxidase was separated into three polypeptides, va, vb and vc with different molecular masses (7.4 kda, 6.8 kda and 6.2 kda respectively) by highly resolving sodium dodecylsulfate polyacrylamide gel electrophoresis. antibody against subunit v reacted specifically with the polypeptide vc. when polyadenylated mrna from sweet potato root tissue was translated in a wheat germ cell-free system, the smallest subunit (vc) of the polypeptides was synt ... | 2010 | 2885192 |
| [study of red color development of sweet potato epidermis by treatment with phosphate preparations]. | | 2012 | 3567432 |
| cf1atpase beta- and epsilon-subunit genes are separated in the sweet potato chloroplast genome. | | 2010 | 2889184 |
| evaluation of several methods for analysis of sweet potato phenolics. | | 1967 | 546955 |
| studies of nitrogen balance in male highlanders in papua new guinea. | nitrogen metabolism in papua new guinea highlanders was studied by examining their nitrogen balance when they ate their usual diet (experiment 1) or an experimental diet (experiment 2). studies were made on 39 male highlanders between october and december in 1980 and 1982. in experiment 1, the average protein and energy intakes (mean +/- sd) for three consecutive days of usual diets were 116.2 +/- 31.2 mg n/kg body weight and 46.9 +/- 8.9 kcal/kg body weight in 1980 and 143.5 +/- 54.8 mg n/kg bo ... | 2014 | 3958801 |
| the facultative symbiont rickettsia protects an invasive whitefly against entomopathogenic pseudomonas syringae strains. | facultative endosymbionts can benefit insect hosts in a variety of ways, including context-dependent roles, such as providing defense against pathogens. the role of some symbionts in defense may be overlooked, however, when pathogen infection is transient, sporadic, or asymptomatic. the facultative endosymbiont rickettsia increases the fitness of the sweet potato whitefly (bemisia tabaci) in some populations through mechanisms that are not yet understood. in this study, we investigated the role ... | 2014 | 25217020 |
| the gcp molecular marker toolkit, an instrument for use in breeding food security crops. | crop genetic resources carry variation useful for overcoming the challenges of modern agriculture. molecular markers can facilitate the selection of agronomically important traits. the pervasiveness of genomics research has led to an overwhelming number of publications and databases, which are, nevertheless, scattered and hence often difficult for plant breeders to access, particularly those in developing countries. this situation separates them from developed countries, which have better endowe ... | 2010 | 22162942 |
| an ipomoea batatas iron-sulfur cluster scaffold protein gene, ibnfu1, is involved in salt tolerance. | iron-sulfur cluster biosynthesis involving the nitrogen fixation (nif) proteins has been proposed as a general mechanism acting in various organisms. nifu-like protein may play an important role in protecting plants against abiotic and biotic stresses. an iron-sulfur cluster scaffold protein gene, ibnfu1, was isolated from a salt-tolerant sweetpotato (ipomoea batatas (l.) lam.) line lm79 in our previous study, but its role in sweetpotato stress tolerance was not investigated. in the present stud ... | 2014 | 24695556 |
| analysis of a sugar response mutant of arabidopsis identified a novel b3 domain protein that functions as an active transcriptional repressor. | a recessive mutation hsi2 of arabidopsis (arabidopsis thaliana) expressing luciferase (luc) under control of a short promoter derived from a sweet potato (ipomoea batatas) sporamin gene (spo(min)luc) caused enhanced luc expression under both low- and high-sugar conditions, which was not due to increased level of abscisic acid. the hsi2 mutant contained a nonsense mutation in a gene encoding a protein with b3 dna-binding domain. hsi2 and two other arabidopsis proteins appear to constitute a novel ... | 2005 | 15894743 |
| isolation of a regulatory gene of anthocyanin biosynthesis in tuberous roots of purple-fleshed sweet potato. | many transcriptional factors harboring the r2r3-myb domain, basic helix-loop-helix domain, or wd40 repeats have been identified in various plant species as regulators of flavonoid biosynthesis in flowers, seeds, and fruits. however, the regulatory elements of flavonoid biosynthesis in underground organs have not yet been elucidated. we isolated the novel myb genes ibmyb1 and ibmyb2s from purple-fleshed sweet potato (ipomoea batatas l. lam. cv ayamurasaki). ibmyb1 was predominantly expressed in t ... | 2007 | 17208956 |
| ibmads1 (ipomoea batatas mads-box 1 gene) is involved in tuberous root initiation in sweet potato (ipomoea batatas). | the tuberization mechanism of sweet potato (ipomoea batatas) has long been studied using various approaches. morphological data have revealed that the tuberizing events result from the activation of the cambium, followed by cell proliferation. however, uncertainties still remain regarding the regulators participating in this signal-transduction pathway. an attempt was made to characterize the role of one mads-box transcription factor, which was preferentially expressed in sweet potato roots at t ... | 2008 | 18463111 |
| the genome of cultivated sweet potato contains agrobacterium t-dnas with expressed genes: an example of a naturally transgenic food crop. | agrobacterium rhizogenes and agrobacterium tumefaciens are plant pathogenic bacteria capable of transferring dna fragments [transfer dna (t-dna)] bearing functional genes into the host plant genome. this naturally occurring mechanism has been adapted by plant biotechnologists to develop genetically modified crops that today are grown on more than 10% of the world's arable land, although their use can result in considerable controversy. while assembling small interfering rnas, or sirnas, of sweet ... | 2015 | 25902487 |
| integrative biological hydrogen production: an overview. | biological hydrogen (h2) production by dark and photo-fermentative organisms is a promising area of research for generating bioenergy. a large number of organisms have been widely studied for producing h2 from diverse feeds, both as pure and as mixed cultures. however, their h2 producing efficiencies have been found to vary (from 3 to 8 mol/mol hexose) with physiological conditions, type of organisms and composition of feed (starchy waste from sweet potato, wheat, cassava and algal biomass). the ... | 2012 | 24426072 |
| implication of the bacterial endosymbiont rickettsia spp. in interactions of the whitefly bemisia tabaci with tomato yellow leaf curl virus. | numerous animal and plant viruses are transmitted by arthropod vectors in a persistent, circulative manner. tomato yellow leaf curl virus (tylcv) is transmitted by the sweet potato whitefly bemisia tabaci. we report here that infection with rickettsia spp., a facultative endosymbiont of whiteflies, altered tylcv-b. tabaci interactions. a b. tabaci strain infected with rickettsia acquired more tylcv from infected plants, retained the virus longer, and exhibited nearly double the transmission effi ... | 2014 | 24600010 |
| biotype characterization, developmental profiling, insecticide response and binding property of bemisia tabaci chemosensory proteins: role of csp in insect defense. | chemosensory proteins (csps) are believed to play a key role in the chemosensory process in insects. sequencing genomic dna and rna encoding csp1, csp2 and csp3 in the sweet potato whitefly bemisia tabaci showed strong variation between b and q biotypes. analyzing csp-rna levels showed not only biotype, but also age and developmental stage-specific expression. interestingly, applying neonicotinoid thiamethoxam insecticide using twenty-five different dose/time treatments in b and q young adults s ... | 2016 | 27167733 |
| influence of additives on the yield and pathogenicity of conidia produced by solid state cultivation of an isaria javanica isolate. | recently, the q biotype of tobacco whitefly has been recognized as the most hazardous strain of bemisia tabaci worldwide, because of its increased resistance to some insecticide groups. as an alternative control agent, we selected an isaria javanica isolate as a candidate for the development of a mycopesticide against the q biotype of sweet potato whitefly. to select optimal mass production media for solid-state fermentation, we compared the production yield and virulence of conidia between 2 su ... | 2014 | 25606006 |
| historical collections reveal patterns of diffusion of sweet potato in oceania obscured by modern plant movements and recombination. | the history of sweet potato in the pacific has long been an enigma. archaeological, linguistic, and ethnobotanical data suggest that prehistoric human-mediated dispersal events contributed to the distribution in oceania of this american domesticate. according to the "tripartite hypothesis," sweet potato was introduced into oceania from south america in pre-columbian times and was then later newly introduced, and diffused widely across the pacific, by europeans via two historically documented rou ... | 2013 | 23341603 |
| complete genome sequence of kosakonia sacchari strain bo-1, an endophytic diazotroph isolated from a sweet potato. | the complete genome sequence of the endophytic diazotroph kosakonia sacchari, isolated from a sweet potato, was analyzed. the 4,902,106-bp genome with 53.7% g+c content comprises 4,638 open reading frames, including nif genes, 84 trnas, and seven complete rrnas in a circular chromosome. | 2016 | 27609910 |
| development of transgenic sweet potato with multiple virus resistance in south africa (sa). | multiple infections of sweet potato feathery mottle virus (spfmv), sweet potato chlorotic stunt virus (spcsv), sweet potato virus g (spvg) and sweet potato mild mottle virus (spmmv) cause a devastating synergistic disease complex of sweet potato (ipomoea batatas lam.) in kwazulu-natal, south africa. in order to address the problem of multiple virus infections and synergism, this study aimed to develop transgenic sweet potato (cv. blesbok) plants with broad virus resistance. coat protein gene seg ... | 2014 | 24158330 |
| transplastomic nicotiana benthamiana plants expressing multiple defence genes encoding protease inhibitors and chitinase display broad-spectrum resistance against insects, pathogens and abiotic stresses. | plastid engineering provides several advantages for the next generation of transgenic technology, including the convenient use of transgene stacking and the generation of high expression levels of foreign proteins. with the goal of generating transplastomic plants with multiresistance against both phytopathogens and insects, a construct containing a monocistronic patterned gene stack was transformed into nicotiana benthamiana plastids harbouring sweet potato sporamin, taro cystatin and chitinase ... | 2014 | 24479648 |
| mycotoxicoses of ruminants and horses. | in the current study, mycotoxicoses of ruminants and horses are reviewed, with an emphasis on the occurrence of these diseases in south america. the main mycotoxicoses observed in grazing cattle include intoxications by indole-diterpenoid mycotoxins (paspalum spp. contaminated by claviceps paspali, lolium perenne infected by neotyphodium lolii, cynodon dactylon infected by claviceps cynodontis, and poa huecu), gangrenous ergotism and dysthermic syndrome (hyperthermia) caused by festuca arundinac ... | 2013 | 24091682 |
| starch saccharification and fermentation of uncooked sweet potato roots for fuel ethanol production. | an energy-saving ethanol fermentation technology was developed using uncooked fresh sweet potato as raw material. a mutant strain of aspergillus niger isolated from mildewed sweet potato was used to produce abundant raw starch saccharification enzymes for treating uncooked sweet potato storage roots. the viscosity of the fermentation paste of uncooked sweet potato roots was lower than that of the cooked roots. the ethanol fermentation was carried out by zymomonas mobilis, and 14.4 g of ethanol ( ... | 2013 | 23219688 |
| statistical optimization of process parameters for exopolysaccharide production by aureobasidium pullulans using sweet potato based medium. | statistical experimental designs were applied to optimize the fermentation medium for exopolysaccharide (eps) production. plackett-burman design was applied to identify the significance of seven medium variables, in which sweet potato and yeast extract were found to be the significant variables for eps production. central composite design was applied to evaluate the optimum condition of the selected variables. maximum eps production of 9.3 g/l was obtained with the predicted optimal level of swe ... | 2015 | 28324414 |
| synergistic effect of photosynthetic bacteria and isolated bacteria in their antifungal activities against root rot fungi. | antifungal bacteria (ab) in root rot fungus (rrf)-contaminated sweet potato farms were isolated, and seven strains were initially chosen as antagonistic candidates. an antagonistic test by using the mycelial disk placement method revealed that one ab strain by itself could inhibit the rrf growth. this ab strain was identified as bacillus polyfermenticus based on phylogeny of 16s ribosomal rna genes. two ab strains (bacillus aerophilus) displayed high levels of antifungal activity when paired wit ... | 2016 | 27667522 |
| production of α-1,4-glucosidase from bacillus licheniformis kibge-ib4 by utilizing sweet potato peel. | in the current study, sweet potato peel (ipomoea batatas) was observed as the most favorable substrate for the maximum synthesis of α-1,4-glucosidase among various agro-industrial residues. bacillus licheniformis kibge-ib4 produced 6533.0 u ml(-1) of α-1,4-glucosidase when growth medium was supplemented with 1% dried and crushed sweet potato peel. it was evident from the results that bacterial isolate secreted 6539.0 u ml(-1) of α-1,4-glucosidase in the presence of 0.4% peptone and meat extract ... | 2017 | 27928754 |
| influencing the monophenolase/diphenolase activity ratio in tyrosinase. | tyrosinase is a type 3 copper enzyme with great potential for production of commercially valuable diphenols from monophenols. however, the use of tyrosinase is limited by its further oxidation of diphenols to quinones. we recently determined the structure of the bacillus megaterium tyrosinase revealing a residue, v218, which we proposed to take part in positioning of substrates within the active site. in the structure of catechol oxidase from ipomoea batatas, the lack of monophenolase activity w ... | 2013 | 23305929 |
| [the effect of phospholipids on starch metabolism]. | the presence of phospholipids reduces the breakdown of amylose catalyzed by β-amylase, phosphorylase and α-amylase. the activities of the β-amylases of sweet potato (ipomoea batatas) and barley (hordeum vulgare l.) disminish to less than 10% of the activity in the control without the phospholipids. when the amylose was complexed with phospholipids the activity of the α-amylase of bacillus subtilis was reduced to about 25% of the control value. a similar effect was observed for the amylases of ze ... | 1976 | 24430907 |
| assembling of holotrichia parallela (dark black chafer) midgut tissue transcriptome and identification of midgut proteins that bind to cry8ea toxin from bacillus thuringiensis. | holotrichia parallela is one of the most severe crop pests in china, affecting peanut, soybean, and sweet potato crops. previous work showed that cry8ea toxin is highly effective against this insect. in order to identify cry8ea-binding proteins in the midgut cells of h. parallela larvae, we assembled a midgut tissue transcriptome by high-throughput sequencing and used this assembled transcriptome to identify cry8ea-binding proteins by liquid chromatography-tandem mass spectrometry (lc-ms/ms). fi ... | 2015 | 26135984 |
| shared binding sites for the bacillus thuringiensis proteins cry3bb, cry3ca, and cry7aa in the african sweet potato pest cylas puncticollis (brentidae). | bacillus thuringiensis cry3bb, cry3ca, and cry7aa have been reported to be toxic against larvae of the genus cylas, which are important pests of sweet potato worldwide and particularly in sub-saharan africa. however, relatively little is known about the processing and binding interactions of these coleopteran-specific cry proteins. the aim of the present study was to determine whether cry3bb, cry3ca, and cry7aa proteins have shared binding sites in cylas puncticollis to orient the pest resistanc ... | 2014 | 25261517 |
| distinct gut microbiota of healthy children from two different geographic regions of thailand. | in thailand, food consumption by people from each region is different. this can be an important environmental factor which shapes the gut microbiota further affecting their health. this study aimed to use quantitative pcr (qpcr) to investigate the intestinal microbial community in 60 healthy children (aged 8-11 years) living in specific areas, namely central (ct) and northeastern (ne) thailand where each region has its own typical food consumption. the children from ne had significantly higher c ... | 2015 | 25644242 |
| nutraceuticals of anti-inflammatory activity as complementary therapy for rheumatoid arthritis. | rheumatoid arthritis (ra) is a chronic inflammatory disease characterized by elevated oxidative stress and inflammatory biomarkers. the severe side effects of drug used during such disease necessitate the search for new and safe approaches. food is a rich source of antioxidants and anti-inflammatory bioactive constituents including phenolic compounds, polyunsaturated fatty acids, phytosterols, toccopherols, and carotenoids. we have a series of publications dealing with the anti-inflammatory acti ... | 2014 | 23104728 |
| symbiotic n 2 -fixation estimated by the (15) n tracer technique and growth of pueraria phaseoloides (roxb.) benth. inoculated with bradyrhizobium strain in field conditions. | this field experiment was established in eastern cameroon to examine the effect of selected rhizobial inoculation on n2-fixation and growth of pueraria phaseoloides. treatments consisted of noninoculated and bradyrhizobium yuanmingense s3-4-inoculated pueraria with three replications each. ipomoea batatas as a non-n2-fixing reference was interspersed in each pueraria plot. all the twelve plots received 2 gn/m(2) of (15)n ammonium sulfate 10% atom excess. at harvest, dry matter yields and the nit ... | 2016 | 26904363 |
| [characterization of ipomoea batatas extract to be used as nutrient basis for culture media]. | an ipomoea batatas extract obtained by enzymatic hydrolysis with alpha amylase was characterized to be used as nutrient basis. among its quality indicators, it had a content of over 50% whole carbohydrates with respect to the nominal mass, estimated by phenol-sulphur method. the same content of aminonitrogen detected and quantified by potentiometric titration using formaldehyde and of total nitrogen (0,23%) by kjeldahl method were detected. the content of necessary mineral elements for microbial ... | 2013 | 23427460 |
| enhanced accumulation of carotenoids in sweetpotato plants overexpressing ibor-ins gene in purple-fleshed sweetpotato cultivar. | sweetpotato [ipomoea batatas (l.) lam] is an important root crop that produces low molecular weight antioxidants such as carotenoids and anthocyanin. the sweetpotato orange (ibor) protein is involved in the accumulation of carotenoids. to increase the levels of carotenoids in the storage roots of sweetpotato, we generated transgenic sweetpotato plants overexpressing ibor-ins under the control of the cauliflower mosaic virus (camv) 35s promoter in an anthocyanin-rich purple-fleshed cultivar (refe ... | 2015 | 25438140 |
| cloning and characterization of an orange gene that increases carotenoid accumulation and salt stress tolerance in transgenic sweetpotato cultures. | the orange (or) gene is responsible for the accumulation of carotenoids in plants. we isolated the or gene (ibor) from storage roots of orange-fleshed sweetpotato (ipomoea batatas l. lam. cv. sinhwangmi), and analyzed its function in transgenic sweetpotato calli. the ibor gene has an open reading frame in the 942 bp cdna, which encodes a 313-amino acid protein containing a cysteine-rich zinc finger domain. ibor was strongly expressed in storage roots of orange-fleshed sweetpotato cultivars; it a ... | 2013 | 23835362 |