| the effect of lectin from taro tuber (colocasia antiquorum) given by force-feeding on the growth of mice. | in earlier experiments in our laboratory, a lectin from the kintoki bean (phaseolus vulgaris) was found to have not only erythrocyte agglutinating activity but also toxicities for mice and rats, including growth inhibitory activity and even lethal activity. a number of studies on legume lectins have been carried out in other laboratories as well. but relatively little attention has been paid to lectins from non-leguminous foods. in the present study, we chose taro tuber as a source of non-legumi ... | 1990 | 2292730 |
| construction of a stable shuttle vector for high-frequency transformation in pseudomonas syringae pv. syringae. | a cryptic 80.3-kilobase plasmid, posu900, in pseudomonas syringae pv. syringae strain j900 could be cured by treatment with mitomycin without affecting the pathogenicity of j900 on the host, phaseolus vulgaris l. the replication region of posu900 was identified, subcloned, and modified for construction of a high-copy cloning vector. this vector could be transformed into pseudomonas strains with high efficiency (ca. 10(6) transformants per microgram of dna) and was very stable during growth of th ... | 1990 | 2294094 |
| arborization of corticothalamic axons in the auditory thalamus of the cat: a pha-l tracing study. | as a result of phaseolus vulgaris leucoagglutinin (pha-l) injection in the primary auditory cortex of the cat, portions of corticofugal axons could be traced individually in the reticular complex of the thalamus (re) and in the medial geniculate body (mgb). on their way to the mgb, axons were seen to give off collaterals which terminated in re. in the mgb, an individual corticofugal axon was fully reconstructed. it ramified at 3 distinct frontal levels in the rostral half of the pars lateralis ( ... | 1990 | 2304635 |
| secretory glycoconjugates of a mucin-synthesizing human colonic adenocarcinoma cell line. analysis using double labeling with lectins. | lectins were used to characterize mucin glycoproteins and other secretory glycoconjugates synthesized by a human colon adenocarcinoma-derived cell line which expresses a goblet cell phenotype. despite being clonally derived, ht29-18n2 (n2) cells, like normal goblet cells in situ were heterogeneous in their glycosylation of mucin. only wheat-germ agglutinin, which recognizes n-acetylglucosamine and sialic acid residues, and succinylated wheatgerm agglutinin, which binds n-acetylglucosamine, stain ... | 1990 | 2312359 |
| cortical projection patterns of the medial septum-diagonal band complex. | a detailed analysis of the cortical projections of the medial septum-diagonal band (ms/db) complex was carried out by means of anterograde transport of phaseolus vulgaris leucoagglutinin (pha-l). the tracer was injected iontophoretically into cell groups of the medial septum (ms) and the vertical and horizontal limbs of the diagonal band of broca (vdb and hdb), and sections were processed immunohistochemically for the intra-axonally transported pha-l. the labeled efferents showed remarkable diff ... | 1990 | 2312788 |
| human milk contains proteins that stimulate and suppress t lymphocyte proliferation. | the modulatory effect of human milk proteins from colostrum and late milk on the proliferative response of human t lymphocytes activated by mitogens (okt3 and leucoagglutinin from phaseolus vulgaris) and alloantigens was studied. high concentrations (10-100 micrograms/ml) of crude colostral milk proteins had an inhibitory effect on t cell growth while low concentrations (0.1-1 microgram/ml) enhanced t cells growth. in contrast, proteins from late milk did not inhibit t lymphocyte proliferation w ... | 1990 | 2317950 |
| branching patterns of corticospinal axon arbors in the rodent. | despite extensive study of corticospinal connections in a variety of species, little is known about the detailed morphology of corticospinal axon arbors. results in previous studies of primates based on intra-axonal filling with horseradish peroxidase (hrp) staining of a limited sample of fibers suggest that corticospinal arbors branch widely to multiple motoneuronal pools. to determine whether this pattern of corticospinal connectivity is present in nonprimate species as well, we studied the br ... | 1990 | 2324314 |
| evidence for a functional and anatomical relationship between the lateral septum and the hypothalamus in the control of flank marking behavior in golden hamsters. | golden hamsters with established dominant/subordinate relationships communicate their social status by rubbing pheromone-producing flank glands against objects in the environment. this behavior, called flank marking, is controlled by vasopressin-sensitive neurons localized to the anterior hypothalamus. vasopressinergic magnocellular neurons in the nucleus circularis and medial aspect of the supraoptic nucleus are thought to be a source of neurotransmitter for the initiation of flank marking. the ... | 1990 | 2324325 |
| demonstration of two separate descending noradrenergic pathways to the rat spinal cord: evidence for an intragriseal trajectory of locus coeruleus axons in the superficial layers of the dorsal horn. | the rat spinal cord receives noradrenergic (na) projections from the locus coeruleus (lc) and the a5 and a7 groups. in contradiction to previous statements about the distribution of descending na axons, we have recently proposed that in the rat lc neurons project primarily to the dorsal horn and intermediate zone, whereas a5 and a7 neurons project to somatic motoneurons and the intermediolateral cell column. the aim of the present study was to determine the funicular course and terminal distribu ... | 1990 | 2329191 |
| distribution of locus coeruleus axons within the rat brainstem demonstrated by phaseolus vulgaris leucoagglutinin anterograde tracing in combination with dopamine-beta-hydroxylase immunofluorescence. | projections of the locus coeruleus (lc) to the midbrain and hindbrain were analyzed by anterograde transport of the lectin phaseolus vulgaris leucoagglutinin (pha-l). following iontophoretic application of pha-l into the lc, the distribution of labeled axons was analyzed in sections processed for the immunoperoxidase method and in sections processed for double-immunofluorescence staining using antibodies to pha-l and to dopamine-beta-hydroxylase. this combined staining approach proved to be nece ... | 1990 | 2329197 |
| lectin staining of saccharides in the normal and hypothyroid developing organ of corti. | lectin staining has been used to detect mono- and oligosaccharides in normal and hypothyroid developing organs of corti in the rat. eight developmental stages were studied (1, 5, 8, 10, 15, 20, 50 and 60 days after birth). congenital hypothyroidism was induced by oral administration of propylthyouracil to pregnant rats. labelling of the tectorial membrane with 3 lectins, ulex europaeus agglutinin-i (uea-i), lens culinaris agglutinin (lca) and ricinus communis agglutinin-i (rca-i) showed no signi ... | 1990 | 2331782 |
| efferent projections of the subthalamic nucleus in the squirrel monkey as studied by the pha-l anterograde tracing method. | the organization of the efferent connections of the subthalamic nucleus was studied in the squirrel monkey (saimiri sciureus) by using the lectin phaseolus vulgaris-leucoagglutinin (pha-l) as an anterograde tracer. at the level of the basal forebrain, anterogradely labeled fibers and axon terminals were mostly found in the striatopallidal complex and the substantia innominata. in cases in which the pha-l injection sites were placed in the central or the lateral third of the subthalamic nucleus, ... | 1990 | 2332533 |
| [antimutagenic activity of vegetable and fruit extracts against in-vitro benzo(a)pyrene]. | about 80% of the juices from twenty vegetables and fruits showed antimutagenic activity when tested in the presence of the mutagen and carcinogen benzo[a]pyrene using the ames' salmonella/microsome assay. in a standardized test system juices from raw celeriac, broccoli, red cabbage, carrots, green, peppers, lettuce, asparagus, apricots, red-currants, gooseberries, raspberries, and pineapple showed more than 50% inhibition. leek, kohlrabi, cucumber, zucchini, french beans, fennel leaves, rhubarb, ... | 1990 | 2336853 |
| electron microscopic evidence of a monosynaptic pathway between cells in the caudal raphé nuclei and sympathetic preganglionic neurons in the rat spinal cord. | electrophysiological and anatomical studies have suggested the existence of a pathway between the caudal raphé nuclei and regions of the spinal cord containing the sympathetic preganglionic neurons. however synaptic connections between cells in the raphé nuclei and identified sympathetic preganglionic neurons have not yet been shown. we have used a combination of anterograde tracing using phaseolus vulgaris leucoagglutinin (pha-l), retrograde tracing using a conjugate of cholera b chain and hrp ... | 1990 | 2340876 |
| diversity of oligosaccharide structures on the envelope glycoprotein gp 120 of human immunodeficiency virus 1 from the lymphoblastoid cell line h9. presence of complex-type oligosaccharides with bisecting n-acetylglucosamine residues. | the n-linked oligosaccharide structures on the envelope glycoprotein gp120 of human immunodeficiency virus 1 derived from chronically infected lymphoblastoid (h9) cells have been investigated by enzymatic microsequencing after release from protein by hydrazinolysis, labeling with nab3h4, and chromatography on adsorbent columns of phaseolus vulgaris erythrophytohemagglutinin and ricinus communis agglutinin (mr 120,000) and on bio-gel p-4. a substantially greater diversity of oligosaccharide struc ... | 1990 | 2341393 |
| connections of the subthalamic nucleus with ventral striatopallidal parts of the basal ganglia in the rat. | the present study was undertaken to establish the precise anatomical relationship of the subthalamic nucleus (sth) with limbic lobe-afferented parts of the basal ganglia in the rat. the anterograde tracer phaseolus vulgaris-leucoagglutinin (pha-l), injected in the sth, the globus pallidus, the ventral pallidum, the ventral striatum, and the parafascicular thalamic nucleus, and the retrograde tracers fluoro-gold (fg) and cholera toxin b (ctb), injected in the globus pallidus, the ventral pallidum ... | 1990 | 2341628 |
| the basal forebrain projection to the region of the nuclei gemini in the rat; a combined light and electron microscopic study employing horseradish peroxidase, fluorescent tracers and phaseolus vulgaris-leucoagglutinin. | we have examined the location of basal forebrain cells projecting to the region of the nuclei gemini in the caudolateral hypothalamus of the rat using retrograde transport of wheatgerm agglutinin-horseradish peroxidase. since many tracer-positive neurons were identified in ventral pallidal areas known to project to the mediodorsal nucleus of the thalamus, we also prepared several animals with wheatgerm agglutinin-horseradish peroxidase injections in mediodorsal thalamus. many of the sections fro ... | 1990 | 2352648 |
| organization of intrahippocampal projections originating from ca3 pyramidal cells in the rat. | the distribution of intrahippocampal projections arising from the ca3 region of the rat hippocampus was investigated using in vitro and in vivo methods. in the in vitro hippocampal slice preparation, single ca3 pyramidal cells were intracellularly labeled with horseradish peroxidase (hrp), and the three-dimensional organization of the axonal plexus was analyzed by using a computer-aided digitizing system. as many as eight primary collaterals originated from the principal axon of ca3 pyramidal ce ... | 1990 | 2358523 |
| projection from the nucleus reuniens thalami to the hippocampal region: light and electron microscopic tracing study in the rat with the anterograde tracer phaseolus vulgaris-leucoagglutinin. | in order to study the morphological substrate of possible thalamic influence on the cells of origin and area of termination of the projection from the entorhinal cortex to the hippocampal formation, we examined the pathways, terminal distribution, and ultrastructure of the innervation of the hippocampal formation and parahippocampal region by the nucleus reuniens of the thalamus (nrt). we employed anterograde tracing with phaseolus vulgaris-leucoagglutinin (pha-l). injections of pha-l in the nrt ... | 1990 | 2358531 |
| hippocampal and midline thalamic fibers and terminals in relation to the choline acetyltransferase-immunoreactive neurons in nucleus accumbens of the rat: a light and electron microscopic study. | the synaptic interactions between terminals of allocorticostriatal and thalamostriatal fibers and the cholinergic neurons in the nucleus accumbens were investigated using degeneration and dual labelling immunocytochemistry in wistar rats. the presumptive cholinergic neurons were labelled with antibodies directed against choline acetyltransferase and the afferent fibers were labelled anterogradely with phaseolus vulgaris-leucoagglutinin. fibers from the subiculum of the hippocampal formation and ... | 1990 | 2358532 |
| iron retention by rats from casein-legume test meals: effect of tannin level and previous diet. | the effect of tannin content on iron (fe) bioavailability from several legumes was evaluated. absorption of fe from a casein (c), isolated soy protein (isp), chickpea (cp) or red kidney bean (rkb) test meal was tested in marginally fe-deficient rats [9.9 +/- 0.2 g hb/100 ml (mean +/- sd)] using the extrinsic tag technique. also, the effects of a casein habitual diet or of one of three casein-legume habitual diets fed before and after the test meal were investigated. weanling male rats were fed t ... | 1990 | 2366110 |
| amygdalonigral pathway: an anterograde study in the rat with phaseolus vulgaris leucoagglutinin (pha-l). | the projection from the central nucleus of the amygdala to the substantia nigra was labeled by injections of the anterograde tracer phaseolus vulgaris leucoagglutinin into different subregions of the nucleus. a sparse projection of labeled bouton-like swellings was observed in the rostral, medial substantia nigra pars compacta and ventral tegmental area from all subregions of the central nucleus of the amygdala that were injected. a dense projection of labeled axons and bouton-like swellings was ... | 1990 | 2370319 |
| termination patterns of serotoninergic medullary raphespinal fibers in the rat lumbar spinal cord: an anterograde immunohistochemical study. | electrical and chemical stimulation given in the ventral medullary raphe nuclei inhibits spinal nociceptive reflexes and spinal nociceptive transmission; serotoninergic receptors have been demonstrated to partially mediate that inhibition. in the present study, the termination patterns of raphespinal fibers in the rat lumbar spinal cord demonstrating serotonin-like immunoreactivity were examined by using the anterograde tracer phaseolus vulgaris leucoagglutinin (pha-l) in combination with immuno ... | 1990 | 2370323 |
| sti35, a stress-responsive gene in fusarium spp. | a stress-induced mrna was identified in the phytopathogenic fungus fusarium oxysporum f. sp. cucumerinum. treatment of the fungus with ethanol resulted in the induction of a major mrna species encoding a protein of approximate mr 37,000. a full-length cdna clone of the induced message was obtained. rna blot analysis indicated that the mrna was induced by various other stresses, including treatment with copper(ii) chloride and heat (37 degrees c). however, it was not greatly induced by treatment ... | 1990 | 2376567 |
| trigeminal projections to contralateral dorsal horn: central extent, peripheral origins, and plasticity. | prior studies have documented a trigeminal (v) mandibular primary afferent projection to the dorsomedial portion of the contralateral medullary and cervical dorsal horns in cat, hamster, and rat. we now report the existence of a much more substantial v ophthalmic primary afferent projection to the ventrolateral portion of contralateral medullary and cervical dorsal horns in rat. horseradish peroxidase (hrp) injections into the v ganglion or v brainstem complex anterogradely labeled a fascicle of ... | 1990 | 2378191 |
| analysis of the oligosaccharides on rat androgen-binding protein using serial lectin chromatography. | the microheterogeneity seen when rat androgen-binding protein (rabp) is analyzed by two-dimensional polyacrylamide gel electrophoresis is attributable, at least in part, to the differential glycosylation of a single promoter. further insight into the chemical nature of the oligosaccharide units on rabp was obtained by serial lectin chromatography. when rabp was chromatographed on immobilized concanavalin a (con-a), it was fractionated into three classes: (1) one that did not bind to the lectin ( ... | 1990 | 2378933 |
| topographical distribution of septohippocampal projections demonstrated by the pha-l immunohistochemical method in rats. | the anterograde tracer, phaseolus vulgaris-leucoagglutinin (pha-l) was injected into the medial septum-diagonal band complex of the rat. when pha-l was injected into the rostral part of the medial septum, labeled axon terminals were distributed largely in the dorsal hippocampus. after pha-l injection into the caudal part of the medial septum or vertical limb of diagonal band of broca, labeled terminals were observed predominantly in the ventral hippocampus. | 1990 | 2381561 |
| afferents to the cerebellar nuclei from the cervical enlargement in the rat, as demonstrated with the phaseolus vulgaris leucoagglutinin method. | injections of phaseolus vulgaris leucoagglutinin into the cervical enlargement labeled spinocerebellar fibers in the rat cerebellar nuclei. immunoreactive terminal axons and terminals were distributed in the medial nucleus, and the anterior interpositus and the posterior interpositus nucleus. the present study suggests that spinocerebellar neurons in the cervical enlargement (the medial lamina vi and the central lamina vii groups) project to the cerebellar nuclei. | 1990 | 2381562 |
| contralateral projections of cells in the motor trigeminal nucleus of the rat. | with horseradish peroxidase and phaseolus vulgaris lectin as tracers, a direct connection between the jaw closing parts of the ipsi- and contralateral motor trigeminal (mo5) nuclei of the rat is shown. the contralateral projecting cells in mo5 were small (18 x 11 microns) ovoid and bipolar. it is speculated that these contralateral projecting cells in mo5 are interneurons that are involved in the coordination of the bilateral activity of jaw closing motoneurons during orofacial motor behavior. | 1990 | 2381563 |
| efferent projections from the lateral septal nucleus to the anterior hypothalamus in the rat: a study combining phaseolus vulgaris-leucoagglutinin tracing with vasopressin immunocytochemistry. | the tracer phaseolus vulgaris-leucoagglutinin (pha-l) was injected into the lateral septum of the rat at different rostrocaudal locations to study the efferent septal projections to the anterior hypothalamus. for spatial correlation of these septofugal elements with the vasopressinergic system a dual immunocytochemical technique was used (i) to demonstrate nerve fibers and their corresponding bouton-like structures labeled with the tracer, and (ii) to identify vasopressin in the same section. th ... | 1990 | 2383883 |
| phaseolus vulgaris leucoagglutinin (pha-l): a neuroanatomical tracer for electron microscopic analysis of synaptic circuitry in the cat's dorsal lateral geniculate nucleus. | phaseolus vulgaris leucoagglutinin (pha-l) is a plant lectin that is anterogradely transported by neurons in the central nervous system. pha-l is selectively taken up by cells at iontophoretic injection sites and, when immunohistochemically demonstrated, labels individual neurons completely, including their dendrites, axons, and terminal boutons. pha-l is generally not taken up by fibers passing through the injection site and, because it produces a golgi-like staining of even very fine axons ove ... | 1990 | 2391562 |
| the subiculum: cytoarchitectonically a simple structure, but hodologically complex. | the subiculum gives rise to the majority of hippocampal projections to various telencephalic and diencephalic structures. previously, these projections have been described using anterograde tracing with radioactively labeled amino acids. as part of an ongoing detailed analysis of the connectivity of the hippocampal region in the rat, we studied the projections of the subiculum by means of the recently introduced sensitive anterograde tracer phaseolus vulgaris-leukoagglutinin (pha-l) and double-l ... | 1990 | 2392570 |
| subicular projections to the hypothalamus and brainstem: some novel aspects revealed in the rat by the anterograde phaseolus vulgaris leukoagglutinin (pha-l) tracing method. | the efferent projections from the subiculum to the hypothalamus were examined in the rat by using the anterograde pha-l tract-tracing method. the density of the subicular projections to the hypothalamus increases at successively more ventral levels of the subiculum. the ventral tip of the region projects to the hypothalamus via 3 different routes: the postcommissural fornix, the medial corticohypothalamic tract (mht) and a ventral pathway running via the amygdala. the fibers in the fornix innerv ... | 1990 | 2392571 |
| antiviral activity of s-adenosylhomocysteine hydrolase inhibitors against plant viruses. | three sah hydrolase inhibitors, (rs)-3-adenin-9-yl-2-hydroxypropanoic acid (isobutyl ester) [(rs)-ahpa]; (rs)-9-(2,3-dihydroxypropyl)adenine [(rs)-dhpa] and the carbocyclic analog of 3-deazaadenosine (c-c3ado) were evaluated for their inhibitory activity against tobacco mosaic virus (tmv) and potato virus x (pvx). using the local lesion assay and elisa, we demonstrated that all three compounds inhibit the replication of tmv and pvx. whereas the three compounds proved about equally active against ... | 1990 | 2396830 |
| concentrations of lead and cadmium in edible vegetables from tarragona province, spain. | the lead and cadmium content of 20 species of edible vegetable collected in tarragona province (spain) was investigated. samples consisting of bulbs, and leaves and soft stalks (chard, parsley, spinach and lettuce) contained the highest levels of both metals. in contrast, fruits and similar garden produce (tomato, green pepper, cucumber, artichoke, green bean and broad bean) contained the lowest concentrations of lead and cadmium in both the northern and southern area of the province. most speci ... | 1990 | 2402626 |
| cytochrome b-245 from human neutrophils is a glycoprotein. | cytochrome b-245 is a glycoprotein. it runs as a broad band on sodium dodecyl sulphate/polyacrylamide-gel electrophoresis and its apparent mr varies with the concentration of acrylamide. it stained positively with schiff reagent and with silver stains after oxidation with periodic acid. it preferentially bound the lectin of phaseolus vulgaris (type iii), and cleavage of carbohydrate with endoglycosidase f resulted in a sharp band on sodium dodecyl sulphate/polyacrylamide-gel electrophoresis with ... | 1985 | 2408606 |
| a projection from the deep layers of the entorhinal area to the hippocampal formation in the rat brain. | the methods of retrograde fluorescent tracing and anterograde transport of the lectin phaseolus vulgaris leucoagglutinin (pha-l) were used to demonstrate the existence of projections from layers iv and vi of the entorhinal area to the hippocampal formation in the rat brain. these two layers of the medial and lateral entorhinal area innervate the molecular layer of ammon's horn and the area dentata. in the area dentata the projection from layer iv follows that of the perforant path, while that fr ... | 1985 | 2409482 |
| analysis of n-linked oligosaccharide chains of glycoproteins on nitrocellulose sheets using lectin-peroxidase reagents. | a rapid and convenient method was established for analysis of the n-linked carbohydrate chains of glycoproteins on nitrocellulose sheets. proteins were separated by polyacrylamide gel electrophoresis, transferred to nitrocellulose sheets, reacted with peroxidase-coupled lectins, and detected by color development of the enzyme reaction. four glycoproteins having n-linked oligosaccharide chains were used as test materials: taka-amylase a (which has a high-mannose-type chain), ovalbumin (high-manno ... | 1985 | 2411164 |
| use of intrinsic antibiotic resistance for characterisation and identification of rhizobia from nodules of vigna unguiculata (l) walp. and phaseolus vulgaris (l). | intrinsic resistance to low concentrations of antibiotics was used to characterise 83 isolates from nodules of cowpea (vigna unguiculata) and field bean (phaseolus vulgaris). characterisation and differentiation of isolates from cowpea was made difficult by associated fast-growing bacteria inside the nodule tissue. thus, reliable pure culture was difficult to secure without repeated isolation and even via nodulation of the appropriate homologous host. although the technique may be satisfactory f ... | 1985 | 2412407 |
| the neostriatal mosaic. i. compartmental organization of projections from the striatum to the substantia nigra in the rat. | combined neuroanatomical techniques were used to examine the organization of the striatal projection to the substantia nigra in the rat. both double anterograde axonal tracing methods (phaseolus vulgaris leuco-agglutinin (pha-l) and 3h-amino acid tract tracing) and double fluorescent retrograde axonal transport tracing methods were used to examine the relationship among striatal neurons projecting to separate areas of the substantia nigra. additionally, the distributions of retrogradely labeled ... | 1985 | 2414339 |
| [isolation of a biologically active poly(a)+rna from tissues enriched with polysaccharides]. | a special technique is developed for isolation of biologically functional poly(a)+rna from higher plant cells to start with some procedures used to isolate rna of the eucaryote cells. the technique includes cell disruption with a 4 m guanidine thiocyanate solution, hot phenol extraction of rna following proteinase k digestion and methoxyethanol treatment to remove polysaccharides. finally, poly(a)+rna was purified by oligo(dt)-cellulose chromatography. using this technique poly(a)+rna preparatio ... | 1985 | 2416103 |
| binding of various lectins during chondrogenesis in mouse limb buds. | the binding of six different fitc-labelled lectins to cells and matrix was investigated during chondrogenesis in mouse limb buds from day 10 to 13 of development. in undifferentiated mesenchyme, concanavalin a and wheat germ agglutinin bound very strongly, whereas at later stages binding was decreased in the peripheral mesenchyme, but very strong in blastemata and cartilage. phaseolus vulgaris lectin showed the same properties, but the decrease in the peripheral mesenchyme was less pronounced. f ... | 1986 | 2422680 |
| allomyrina dichotoma lectin-nonreactive alpha-fetoprotein in hepatocellular carcinoma and other tumors: comparison with ricinus communis agglutinin-i. | allomyrina dichotoma lectin (allo a) with a specificity to beta-d-galactose was used to fractionate human alpha-fetoprotein (afp) by affinity electrophoresis. afp from cord sera and serum of a patient with fulminant hepatitis showed single bands with a high affinity for allo a. some patients with hepatocellular carcinoma and patients with gastric cancer and yolk sac tumor had two additional afp bands, one weakly reactive and the other nonreactive with allo a. patterns of afp bands obtained with ... | 1986 | 2424591 |
| cholinergic and non-cholinergic projections from the rat basal forebrain revealed by combined choline acetyltransferase and phaseolus vulgaris leucoagglutinin immunohistochemistry. | a two-color fluorescence method is described for demonstrating immunohistochemically the anterogradely transported plant lectin phaseolus vulgaris leucoagglutinin (phal, fluorescein isothiocyanate label) and choline acetyltransferase (chat, rhodamine label) on the same rat brain section. application of this method to the study of projection neurons in the vertical and horizontal limbs of the diagonal band, the substantia innominata and nucleus basalis revealed that both cholinergic and non-choli ... | 1986 | 2425289 |
| further resolution of human alpha-fetoprotein by affinity electrophoresis with erythroagglutinating phytohemagglutinin of phaseolus vulgaris lectin. | major molecular species of human alpha-fetoprotein(afp), which were separated as single components by serial affinity chromatography with concanavalin a(con-a) and lens culinaris agglutinin, were further resolved into several bands by affinity electrophoresis with erythroagglutinating phytohemagglutinin of phaseolus vulgaris lectin(e-pha). among the newly separated main molecular species, both con-a- and e-pha-reactive afp(afp-1x1) was demonstrated, contrary to the known sugar specificity of con ... | 1985 | 2426755 |
| increased asialo-alpha-fetoprotein in patients with alpha-fetoprotein-producing tumors: demonstration by affinity electrophoresis with erythroagglutinating phytohemagglutinin of phaseolus vulgaris lectin. | affinity electrophoresis of human alpha-fetoprotein (afp) with the erythroagglutinating phytohemagglutinin of phaseolus vulgaris (e-pha) gave up to five resolved bands (y, h, i, l and a; given in the order of decreasing affinity for e-pha); band a having no affinity and bands y and h representing asialo-afp's. the proportion of band y increased in extrahepatic tumors producing afp, including yolk sac tumor, and of band h, in addition, in hepatocellular carcinoma. the proportion of either band y ... | 1985 | 2426756 |
| studies on glomerular basement membrane in experimental diabetes using lectin histochemistry in wistar rats. | this study was designed to establish whether specific early changes in carbohydrate content of proteins in the glomerulus of the diabetic rat could be detected. lectin staining of kidney sections from streptozotocin-induced diabetic rats were compared with similar sections from healthy and diabetic rats that were treated with insulin. animal groups were killed 1 month, 3 months and 6 months after induction of diabetes. there were no differences in the staining of the glomerular basement membrane ... | 1986 | 2428692 |
| lectin-binding pattern in parotid acinar cells. the fracture-labelling method and post-embedding staining. | the localization of complex carbohydrates in the golgi apparatus, secretory granules and plasmalemma of mouse parotid acinar cells was studied using the fracture-labelling method. the hexose residues of glycoconjugates were identified using ferritin conjugated with wheat germ agglutinin (wga-), ricinnus communis agglutinin ii (rca-ii-), phaseolus vulgaris agglutinin (pha-) and limulus polyphemus agglutinin (lpa-). we found that the fracture-labelling method allows not only the labelling of membr ... | 1986 | 2430921 |
| significance of a common epitope of plant and animal endomembranes. | a rat monoclonal antibody that was raised against a common epitope on bean (phaseolus vulgaris) endomembranes has been shown to cross-react with microsomal polypeptides from a number of plant and animal species. immunoblotting has shown that the epitope is present on a large subset of polypeptides on microsomes of five animal species. the antigenic site appears to be accessible on intact bean membranes since it is readily digested by trypsin. the epitope is probably not derived post-translationa ... | 1986 | 2432075 |
| cholinergic neurons of the laterodorsal tegmental nucleus: efferent and afferent connections. | the ascending projections of cholinergic neurons in the laterodorsal tegmental nucleus (tld) were investigated in the rat by using phaseolus vulgaris leucoagglutinin (pha-l) and wheat germ agglutinin-conjugated horseradish peroxidase (wga-hrp) anterograde tracing techniques. two ascending pathways were identified after iontophoretic injections of pha-l into the tld. a long projection system courses through the dorsomedial tegmentum, caudal diencephalon, medial forebrain bundle, and diagonal band ... | 1986 | 2432101 |
| phaseolus vulgaris leuco-agglutinin tracing of intrahypothalamic connections of the lateral, ventromedial, dorsomedial and paraventricular hypothalamic nuclei in the rat. | intrahypothalamic connections of the lateral (lha), ventromedial (vmh), dorsomedial (dmh) and paraventricular (pvn) hypothalamic nuclei were studied with anterograde transport of iontophoretically injected phaseolus vulgaris leuco-agglutinin and the immunocytochemical detection of labeled structures. the lha was found to give rise to a minor projection in the vmh, whereas the vmh in reverse maintains few connections with the ventromedial part of the tuberal lha. tracer deposits in both the lha a ... | 1987 | 2436726 |
| efferent projections of the subthalamic nucleus in the rat: light and electron microscopic analysis with the pha-l method. | efferent projections of rat subthalamic nucleus were studied by use of the axonal transport of phaseolus vulgaris-leucoagglutinin (pha-l), and the results were analyzed with light and electron microscopes. pha-l injections in the subthalamic nucleus (sth) resulted in heavy labeling of fiber plexus with en passant boutons and terminals in the pallidal complex, i.e., the entopeduncular nucleus (ep), the globus pallidus (gp) and the ventral pallidum (vp), and the substantia nigra pars reticulata (s ... | 1987 | 2439552 |
| double-label immunocytochemistry: combination of anterograde neuroanatomical tracing with phaseolus vulgaris leucoagglutinin and enzyme immunocytochemistry of target neurons. | by the neuroanatomical tracing technique based on uptake, transport, and immunocytochemical detection of injected phaseolus vulgaris leucoagglutinin (pha-l), fiber trajectories of labeled neurons can be followed with great accuracy to their termination areas. to further analyze the connectivity of these fibers, the target neurons must be chemically characterized. in vibratome and frozen sections of rat brain, we tried to visualize pha-l-labeled fibers and, simultaneously, the target neuron-relat ... | 1987 | 2439583 |
| organization of galanin-immunoreactive inputs to the paraventricular nucleus with special reference to their relationship to catecholaminergic afferents. | immunohistochemical and axonal transport techniques were used to characterize the origin and distribution of galanin-immunoreactive inputs to the paraventricular (pvh) and supraoptic (so) nuclei of the hypothalamus in the rat. in the parvicellular division of the pvh, the most prominent inputs were confined to the anterior and periventricular parts of the nucleus rostrally and the dorsal and ventral medial subdivisions caudally; the galaninergic inputs to the magnocellular division of pvh and so ... | 1987 | 2440918 |
| anatomical evidence of a reciprocal connection between the posterior thalamic nucleus and the parvocellular division of the red nucleus in the rat. a combined retrograde and anterograde study. | the functional and anatomical organizations of the magnocellular part of the red nucleus are now well established. our knowledge of the parvocellular part is, however, more limited. using both anterograde and retrograde tracing methods, the present study suggests in the rat the existence of a large projection from the posterior thalamic nucleus to the parvocellular part of the red nucleus. in turn, the parvocellular part of the red nucleus sends a weaker projection to the posterior thalamic nucl ... | 1987 | 2441316 |
| two host-inducible genes of rhizobium fredii and characterization of the inducing compound. | random transcription fusions with mu d1(kan lac) generated three mutants in rhizobium fredii (strain usda 201) which showed induction of beta-galactosidase when grown in root exudate of the host plants glycine max, phaseolus vulgaris, and vigna ungliculata. two genes were isolated from a library of total plasmid dna of one of the mutants, 3f1. these genes, present in tandem on a 4.2-kilobase hindiii fragment, appear in one copy each on the symbiotic plasmid and do not hybridize to the rhizobium ... | 1988 | 2447061 |
| the efferent projections of the rat lateral habenular nucleus revealed by the pha-l anterograde tracing method. | the efferent connections of the rat lateral habenular nucleus (lhb) were demonstrated using anterograde transport of the lectin phaseolus vulgaris leucoagglutinin (pha-l). following pha-l injections into the lhb, neuronal somata located in the lateral two thirds of the lhb were labeled with pha-l. individual axonal fibers and terminal specializations were clearly visible. this permitted detailed mapping of both efferent fiber pathways and terminal distributions. previous reports on fiber pathway ... | 1988 | 2451982 |
| the basolateral amygdaloid complex as a cortical-like structure. | the thalamic innervation of the rat basolateral amygdaloid complex was studied with a combination of light- and electron microscopic techniques using anterogradely transported phaseolus vulgaris-leucoagglutinin (pha-l) as well as combined degeneration and single-section golgi impregnation for the identification of thalamo-amygdaloid synaptic relations. the results indicated that the basolateral amygdaloid nucleus corresponds in several features to a cortical structure. like all cortical areas, t ... | 1988 | 2451985 |
| organization of the afferent connections of the mediodorsal thalamic nucleus in the rat, related to the mediodorsal-prefrontal topography. | the aim of the present study was to determine the organization of the afferents of the mediodorsal thalamic nucleus in relation to the reciprocal connections of this nucleus with different areas of the prefrontal cortex. for this purpose injections of horseradish peroxidase, or horseradish peroxidase conjugated to wheatgerm agglutinin were placed in different parts of the mediodorsal nucleus. these experiments revealed the organization of the reciprocal mediodorsal-prefrontal connections since w ... | 1988 | 2452377 |
| retrograde transport of the lectin phaseolus vulgaris leucoagglutinin (pha-l) by rat spinal motoneurons. | the lectin phaseolus vulgaris leucoagglutinin (pha-l) has been used primarily as an anterograde transport tracer in the cns. we present evidence of pha-l retrograde transport by rat spinal motoneurons after injection into the triceps brachii. labelled motoneurons were localized in specific and well-defined neuron pools in the ventral horn. primary afferent labelling was not seen in the spinal gray matter. dorsal rhizotomy did not eliminate or decrease motoneuron labelling. the retrograde transpo ... | 1988 | 2453002 |
| developmental changes in the molecular weights of polypeptides in the human cns that carry the hnk-1 epitope and bind phaseolus vulgaris lectins. | the binding patterns of electrophoresed polypeptides from homogenates of human frontal lobe, cerebellum, and spinal cord obtained at various stages of development were determined for several lectins with specificities for a wide range of oligosaccharides. a discrete developmental change in the molecular-weight pattern was seen only among polypeptides binding the two phaseolus vulgaris agglutinins, e-phytohemagglutinin (e-pha) and l-pha. with increasing maturity, the apparent molecular weights of ... | 1988 | 2453614 |
| presence of crossed corticorubral fibers and increase of crossed projections after unilateral lesions of the cerebral cortex of the kitten: a demonstration using anterograde transport of phaseolus vulgaris leucoagglutinin. | brain lesions made during early developmental stages produce more prominent remodeling of synaptic organization than those made in adults. this difference in the extent of neuronal or synaptic plasticity between immature and mature animals may be due to difference in the capacity for axonal elongation. alternatively, it could be due to the prevention of retraction of exuberant projections present only in the early developmental stages. aberrant crossed corticorubral projections seen after neonat ... | 1988 | 2454705 |
| innervation of histaminergic neurons in the posterior hypothalamic region by medial preoptic neurons. anterograde tracing with phaseolus vulgaris leucoagglutinin combined with immunocytochemistry of histidine decarboxylase in the rat. | by means of anterograde neuroanatomical tracing with phaseolus vulgaris leucoagglutinin (pha-l) combined with immunohistochemistry of histidine decarboxylase (hdc), we studied in the rat whether the histaminergic neurons in the posterior hypothalamic region are innervated by fibers arising from neurons in the medial preoptic region (mpo). we injected the tracer at various locations in the mpo. following survival, frozen brain sections were dual-stained according to a protocol using pha-l and hdc ... | 1988 | 2458167 |
| anatomical specificity of noradrenergic inputs to the paraventricular and supraoptic nuclei of the rat hypothalamus. | the distribution of neural inputs to the paraventricular (pvh) and supraoptic (so) nuclei from the regions of the a1, the a2, and the a6 (locus coeruleus) noradrenergic cell groups was investigated by using a plant lectin, phaseolus vulgaris leucoagglutinin (pha-l), as an anterogradely transported tracer. an immunofluorescence double-labeling procedure was used to determine the extent to which individual anterogradely labeled fibers and terminals in the pvh and the so also displayed immunoreacti ... | 1988 | 2458397 |
| anterograde and retrograde axonal transport of phaseolus vulgaris leucoagglutinin (pha-l) from the globus pallidus to the striatum of the rat. | iontophoretic administration of pha-l into the globus pallidus of rats resulted in the labeling of neuronal perikarya in the striatum as well as axons and terminals in the striatum, entopeduncular nucleus, subthalamus and substantia nigra. the labeled striatal perikarya were densely stained in golgi fashion with virtually complete filling of the dendrites and spines. it is concluded that the striatal cells were filled by the retrograde transport of pha-l and represent either striatopallidal cell ... | 1988 | 2459567 |
| neural associations of the substantia innominata in the rat: afferent connections. | the afferent connections of the substantia innominata (si) in the rat were determined employing the anterograde axonal transport of phaseolus vulgaris leucoagglutinin (pha-l) and the retrograde transport of wheat germ agglutinin conjugated to horseradish peroxidase (wga-hrp), in combination with histochemical procedures to characterize the neuropil of the si and identify cholinergic cells. both neurochemical and connectional data establish that the si is organized into a dorsal and a ventral div ... | 1988 | 2461972 |
| efferent connections of the substantia innominata in the rat. | the efferent connections of the substantia innominata (si) were investigated employing the anterograde axonal transport of phaseolus vulgaris leucoagglutinin (pha-l) and the retrograde transport of wheat germ agglutinin conjugated to horseradish peroxidase (wga-hrp). the projections of the si largely reciprocate the afferent connections described by grove (j. comp. neurol. 277:315-346, '88) and thus further distinguish a dorsal and a ventral division in the si. efferents from both the dorsal and ... | 1988 | 2461973 |
| pha-l can be transported anterogradely through fibers of passage. | iontophoretic injections of the anterograde tracer phaseolus vulgaris leucoagglutinin (pha-l) were restricted to the dorsal columns in the spinal cords of 9 rats. in 5 of these, labeling was found in the dorsal column nuclei, indicating that fibers of passage can transport pha-l. however, labeled fibers were less dense and less intensely stained than those seen following similar injections that involved the dorsal horn. | 1988 | 2463043 |
| ascending and descending projections of the nucleus reticularis gigantocellularis in the cat demonstrated by the anterograde neural tracer, phaseolus vulgaris leucoagglutinin (pha-l). | ascending and descending projections of the nucleus reticularis gigantocellularis (nrgc) were studied in the cat by the anterograde tracer, phaseolus vulgaris leucoagglutinin. ascending fibers from the left or the right nrgc coursed through the bilateral medial reticular formation and some of them reached the diencephalon. in the brainstem, pha-l-labeled fibers and their terminals were observed in the medial reticular formation, the cranial motor nuclei (iii, iv, v, vi, vii, xii), the vestibular ... | 1988 | 2464400 |
| ascending projections from the pedunculopontine tegmental nucleus and the adjacent mesopontine tegmentum in the rat. | ascending projections from the pedunculopontine tegmental nucleus (ppt) and the surrounding mesopontine tegmentum to the forebrain in the rat are here examined by using both retrograde and anterograde tracing techniques combined with choline acetyltransferase (chat) immunohistochemistry. the anterogradely transported lectin phaseolus vulgaris-leukoagglutinin (pha-l) was iontophoretically injected into the ppt in 12 rats. anterogradely labelled fibers and varicosities were observed in the thalami ... | 1988 | 2464621 |
| glycoconjugates in normal human kidney. a histochemical study using 13 biotinylated lectins. | the avidin-biotin-peroxidase complex technique was used with 13 lectins to study the glycoconjugates of normal human renal tissue. the evaluated lectins included triticum vulgaris (wga), concanavalin ensiformis (cona), phaseolus vulgaris leukoagglutinin and erythroagglutinin (pha-l and pha-e), lens culinaris (lca), pisum sativum (psa), dolichos biflorus (dba), glycine max (sba), arachis hypogaea (pna), sophora japonica (sja), bandeiraea simplicifolia i (bsl-i), ulex europaeus i (uea-i) and ricin ... | 1988 | 2466021 |
| lectin-binding sites in squamous cell carcinomas and keratoacanthomas. | the lectin-binding pattern of malignant and benign human epidermal tumours was studied. twelve squamous cell carcinomas and ten kerato-acanthomas were investigated, using fluorochrome-conjugated lectins. lectins used were canavalia ensiformis agglutinin (con-a), helix pomatia agglutinin (hpa), phaseolus vulgaris agglutinin (pha-l), and ricinus communis agglutinin (rca-120). two types of fluorescence pattern, intercellular 'pemphigus-like' and peritumoral, were noted. both allowed classification ... | 1988 | 2467485 |
| arborization of single axons of the spinal dorsolateral funiculus to the contralateral superficial dorsal horn. | this study provides an anatomical basis for the observation that a unilateral lesion of the spinal dorsolateral funiculus (dlf) can reduce the inhibitory effect of electrical stimulation of the nucleus raphe magnus (nrm) on dorsal horn nociceptive neurons located caudal and contralateral to the lesion. we injected the anterograde tracer phaseolus vulgaris leucoagglutinin (pha-l) into the nrm and traced the arborization of single dlf axons in the spinal gray matter. although the majority of dlf a ... | 1989 | 2467725 |
| muscle and liver protein synthesis and degradation in growing rats fed a raw field bean (vicia faba l.) diet. | body weight gain, food intake, gastrocnemius muscle and liver weight, protein and rna content, as well as the fractional rates of muscle and liver protein synthesis (ks, according to the method of constant infusion of l-[14c]tyrosine), growth (kg) and degradation (kd), along with rna activity (g of protein synthesized per day/g rna) of both organs, were determined in growing male rats fed ad libitum over a period of 10 days on 18.7% protein diets containing either casein (5% of methionine added) ... | 1988 | 2469112 |
| cytochemical localization of carbohydrates in intercalated duct and acinar cells of mouse parotid gland. | the glycoconjugate composition of mouse intercalated duct and acinar cells of parotid gland has been compared. mucins containing 1,2-glycols were demonstrated by the tannic acid-uranyl acetate technique. hexose residues of glycoconjugates were identified using ferritin conjugated with canavalia ensiformis agglutinin (con a), triticum vulgare or wheat germ agglutinin (wga), ricinus communis i agglutinin (rca-i), phaseolus vulgaris agglutinin (pha-e) and arachis hypogaea agglutinin (pna). whereas ... | 1989 | 2470700 |
| a circumscribed projection from the nucleus of the solitary tract to the nucleus ambiguus in the rat: anatomical evidence for somatostatin-28-immunoreactive interneurons subserving reflex control of esophageal motility. | axonal transport and immunohistochemical methods were used to investigate the anatomical and biochemical organization of projections from the nucleus of the solitary tract (nts) to the rostral, esophageal, part of the nucleus ambiguus (na) in the rat. discrete iontophoretic deposits of a retrogradely transported tracer, fluorogold, placed in the rostral na labeled a column of cells within the nts, termed the central part of the nts (after ross et al., 1985), situated just medial to the solitary ... | 1989 | 2470875 |
| axonal arborizations of a magnocellular basal nucleus input and their relation to the neurons in the thalamic reticular nucleus of rats. | a dense axonal plexus, arising in a portion of the magnocellular basal nucleus, was identified in the thalamic reticular nucleus in adult rats. the details of these axonal arbors as well as their relation to the neurons of the reticular nucleus were investigated by using phaseolus vulgaris leucoagglutinin injections into the basal nucleus and intracellular injections of lucifer yellow into reticular nucleus neurons. axons arising in the caudal basal nucleus at the medial margin of the globus pal ... | 1989 | 2471979 |
| new substrate specificity of modified porcine pancreatic alpha-amylase. | conversion of the substrate specificity of porcine pancreatic alpha-amylase (ppa) was studied using chemical modification of his residues. diethyl pyrocarbonate modified his residues in ppa and the activity of the modified ppa for the hydrolysis of the alpha-d-(1,4)glucoside bond in starch or oligosaccharides decreased to less than 1% of that of the native enzyme. however, the activity for the hydrolysis of the bond between p-nitrophenol and oligosaccharides in p-nitrophenyl oligosaccharides was ... | 1989 | 2473713 |
| neurons of the substantia nigra reticulata receive a dense gaba-containing input from the globus pallidus in the rat. | the lectin phaseolus vulgaris leucoagglutinin (pha-l) was used as an anterograde tracer to study the topographical distribution and synaptic organization of pallidonigral fibres in the rat. injections of pha-l in the lateral part of the globus pallidus led to anterograde labelling of a rich plexus of varicose fibres that arborized profusely in the central core of the rostral three quarters of the substantia nigra pars reticulata (snr). however, few fibres were detected in snr after pha-l injecti ... | 1989 | 2476197 |
| the 'marginal division': a new subdivision in the neostriatum of the rat. | using a combination of anterograde and retrograde (phaseolus vulgaris leucoagglutinin; pha-l and wheat germ agglutinin conjugated horseradish peroxidase; wga-hrp) tract-tracing methods and histochemical techniques, a new subdivision of the neostriatum, the marginal division, has been found in the rat brain. the marginal division is approximately 120 microns wide and is located at the caudal extent of the neostriatum and surrounds the rostral edge of the globus pallidus. the neuronal somata of th ... | 1988 | 2477034 |
| host afferents into intrastriatal transplants of fetal ventral mesencephalon. | host afferents into fetal ventral mesencephalic tissue grafted to the neostriatum of adult rats have been studied by using anterograde transport of phaseolus vulgaris leucoagglutinin (pha-l) and immunocytochemistry for serotonin (5-ht), substance p (sp), and dopamine-adenosine 3':5'-monophosphate-regulated-phosphoprotein-32 (darpp-32). numerous fibers of cortical origin were detected in the transplants following multiple (11-15) iontophoretic injections of pha-l into the frontal and anterior cin ... | 1989 | 2477271 |
| supraoptic nucleus afferents from the main olfactory bulb--i. anatomical evidence from anterograde and retrograde tracers in rat. | the morphological features of a putative connection between the main olfactory bulb and the supraoptic nucleus of the rat was studied using a combination of anatomical techniques. immunocytochemistry of neurophysin-containing processes were employed to delineate morphological features of supraoptic dendrites. main olfactory bulb efferents to the supraoptic nucleus were studied by injection of the anterogradely transported substances, wheatgerm agglutinin conjugated horseradish peroxidase or phas ... | 1989 | 2477769 |
| secondary vestibulocerebellar mossy fiber projection to the caudal vermis in the rabbit. | the vestibulocerebellar projection in the rabbit has been investigated by the anterograde axonal transport of tritiated leucine, wheat germ-agglutinated horseradish peroxidase, and phaseolus vulgaris leucoagglutinin. mossy fiber terminals originating from all vestibular nuclei, with the exception of the lateral vestibular nucleus of deiters, were found bilaterally in the lobules x, ix, and viii of the caudal vermis, without a clear difference in laterality. most of the vestibular mossy fiber ter ... | 1989 | 2480371 |
| diverse afferents converge on the nucleus paragigantocellularis in the rat ventrolateral medulla: retrograde and anterograde tracing studies. | the nucleus paragigantocellularis in the ventrolateral medulla has been implicated in cardiovascular, pain, and analgesic functions; and it has also been found to be a major afferent to the pontine nucleus locus coeruleus. in the present study, afferents to the nucleus paragigantocellularis were identified in the rat by means of the retrograde tracers wheat germ agglutinin-conjugated horseradish peroxidase or fluoro-gold. projections to the nucleus paragigantocellularis arise from a wide variety ... | 1989 | 2482306 |
| [electrophoretic analysis of urinary glycoproteins in diabetic nephropathy using peroxidase-lectins]. | we examined the clinical usefulness determined by polyacrylamide gel electrophoresis, followed by reaction with peroxidase-coupled lectins using urinary glycoproteins for diabetic nephropathy in 20 patients with diabetes mellitus. lectins used were triticum vulgaris (wga), phaseolus vulgaris (pha-e4), dolichos biflorus (dba), and lens culinaris (lca), which have high affinity for beta 1----4n-acetyl-d-glucosamine (glcnac beta 1----4glcnac), n-acetyl-d-galactosamine (galnac), alpha-galactosamine ... | 1989 | 2483179 |
| collateralization of the cervical corticospinal tract in the rat. | anterograde staining with phaseolus vulgaris leucoagglutinin (pha-l) revealed the spinal arborization pattern of corticospinal tract (cst) fibers in the cervical enlargement of the rat. within the confines of the pyramidal tract local nets of small fibers are present in addition to the rather large cst fibers with varicosities. cst termination is primarily located in lamina iv and extends into lamina v and vi. extensive collateralization of cst axons was found interconnecting neurons located bot ... | 1989 | 2485885 |
| isolation and partial characterization of the cell membrane of walker-256 carcinosarcoma ascites cells. | cell membranes of carcinosarcoma-256 ascites cells were isolated by the zn++ method. neutral sugars, sialic acid, fucose and n-acetylhexosamines were determined in the cell membrane fraction. cells were agglutinated by wheat germ agglutinin, concanavalin a, and the lectins from phaseolus vulgaris and glycine max. no agglutination was observed with peanut lectin (arachis hypogaea). gel electrophoresis under dissociating conditions revealed more than 40 bands in the membrane fraction after silver ... | 1989 | 2486452 |
| bistratified distribution of terminal arbors of individual axons projecting from area v1 to middle temporal area (mt) in the macaque monkey. | in the present study, the anterograde tracer phaseolus vulgaris-leucoagglutinin was injected into area v1 in order to demonstrate the detailed morphology of individual axons terminating in prestriate area mt. on the basis of 24 axon reconstructions, several representative (but not necessarily comprehensive) characteristics have been identified: (1) most axons arborize in a patchy manner over a widespread territory, frequently greater than 1.0 mm and often up to 1.5 x 1.8 mm (dimensions uncorrect ... | 1989 | 2487098 |
| [food-borne botulism and its epidemiological features as seen in our country during the last few years]. | we evaluated and presented here, the features of the eleven cases of food-borne botulism who admitted to the infectious diseases department of ibni sina hospital of ankara university. three of the cases admitted in 1984 and eight of them admitted in 1987. all of the cases were caused by home-prepared foods which had been eaten without cooking; the types of the food were green beans and pepper with sauce. in these cases the main symptoms and signs were generalized muscular weakness, dry mouth, dy ... | 1989 | 2488935 |
| [fortification of bread with bean flour (phaseolus vulgaris). ii. nutritive value of the fortified bread]. | the protein value of breads containing bean flour (bf) (phaseolus vulgaris, cv tórtola) was tested in rats. bf replaced wheat flour by 2, 4, 6, 8 and 10 per cent. protein content was 9.3 (n x 5.7) per cent and 24.4 (n x 6.25) per cent for wheat flour and bean flour, respectively. the protein content in the control bread was 11.6%, and increased to 12.6% at the 10% level of substitution. parameters measured were protein efficiency ratio (per), net protein ratio (npr), and apparent and true digest ... | 1989 | 2490899 |
| expression of dna binding proteins in carrot somatic embryos that specifically interact with a cis regulatory element of the french bean phaseolin gene. | nuclear proteins from bean (phaseolus vulgarus) embryos bind specifically to a 55 bp dna sequence located upstream of the seed storage protein gene phaseolin. this sequence is capable of elevating gene expression in transgenic tobacco plants by as much as 150-fold when fused to a chimeric beta-glucuronidase reporter gene. results presented in this paper demonstrate that nuclear extracts from carrot embryos bind to a phaseolin dna sequence that includes a phaseolin activator sequence. this specif ... | 1989 | 2491678 |
| adherence of pseudomonas aeruginosa to tracheal epithelium. | adherence of mucoid and nonmucoid strains of pseudomonas aeruginosa to tracheal epithelium was studied with a perfused-trachea model. the species specificity of adherence was studied by infecting tracheas from hamsters, guinea pigs, or mice. perfused tracheas from hamsters were infected with strains of p. aeruginosa in the presence of various sugars, lectins, cations, or charged polymers. adherence of mucoid strains of p. aeruginosa was greatest for guinea pigs; that for hamsters and mice was ap ... | 1989 | 2494109 |
| antibacterial activity of hen egg white lysozyme against listeria monocytogenes scott a in foods. | egg white lysozyme killed or prevented growth of listeria monocytogenes scott a in several foods. lysozyme was more active in vegetables than in animal-derived foods that we tested. for maximum activity in certain foods, edta was required in addition to lysozyme. lysozyme with edta effectively killed inoculated populations of 10(4) l. monocytogenes per g in fresh corn, fresh green beans, shredded cabbage, shredded lettuce, and carrots during storage at 5 degrees c. control incubations without ly ... | 1989 | 2494938 |
| phytoalexin synthesis in soybean cells: elicitor induction of reductase involved in biosynthesis of 6'-deoxychalcone. | chromatofocusing on mono p proved to be an efficient purification procedure for the nadph-dependent reductase from soybean (glycine max l.) cell cultures which acts together with chalcone synthase in the biosynthesis of 2',4',4-trihydroxychalcone (6'-deoxychalcone). by isoelectric focusing the pi of reductase was determined to be 6.3. addition of pure soybean reductase to cell-free extracts from stimulated cell cultures of parsley and bean (phaseolus vulgaris) and from young flowers of dahlia va ... | 1989 | 2500065 |
| survey of some cereal grains and legume seeds for aflatoxin contamination in the sudan. | aflatoxins were detected in some cereals and leguminous seeds collected from 2 cities in the sudan (wad medani and khartoum). the levels of the aflatoxins in these seeds were below the reported hazard threshold. sound intact seeds contained low or no aflatoxins compared to significantly higher levels detectable in damaged and mouldy seeds of the tested crop plants. the optimum temperature for aflatoxin production was variable for different isolates of a. flavus. the optimum is 30 degrees c for t ... | 1989 | 2501947 |
| alpha 2,3-linked sialic acids are more abundant in cd45 antigens and leukosialins of abnormal t cells of lpr mice than in those of normal t cells. | t cells from enlarged lymph nodes of mrl/mpj-lpr/lpr (lpr) mice were found to express more binding sites for strongly hemagglutinating phaseolus vulgaris agglutinin (pha-e4) and fewer binding sites for ricinus communis aglutinin (rca) than those from normal mrl/mpj-+/+ (+/+) mouse lymph node. we found that high-molecular-weight (180k-220k) glycoproteins on lpr t cells were strongly stained with these lectins on western-blotting. these glycoproteins were found to belong to the cd45 family, by abs ... | 1989 | 2534127 |
| structural assessment of the n-linked oligosaccharides of cell-cam 105 by lectin-agarose affinity chromatography. | the n-linked oligosaccharides of cell-cam 105, a glycoprotein involved in the intercellular adhesion between rat hepatocytes, were studied by sequential lectin-agarose affinity chromatography of desialylated, [14c]-labelled glycopeptides. these glycopeptides were obtained by extensive pronase digestion followed by n-[14c]acetylation of the peptide moieties and desialylation by mild acid hydrolysis. assuming that all glycopeptides were radiolabelled to the same specific radioactivity, concanavali ... | 1989 | 2535484 |
| intestinal sphingolipid excretion associated with feeding of phytohemagglutinin lectin (phaseolus vulgaris) to germ-free and conventional rats. | intestinal sphingolipids of feces of germ-free and conventional rats were analyzed during the pair feeding of a complete defined diet containing phytohemagglutinin lectin (pha) from red kidney beans (phaseolus vulgaris) as 1% dietary protein in comparison to casein fed controls. phytohemagglutinin in the diet increased the total fecal excretion of sphingomyelins (18-fold for germ-free and 20-fold for conventional rats), of non-acid glycosphingolipids (3.5-fold for germ-free and 9-fold for conven ... | 1989 | 2535498 |
| cotyledon nuclear proteins bind to dna fragments harboring regulatory elements of phytohemagglutinin genes. | the effects of deleting dna sequences upstream from the phytohemagglutinin-l gene of phaseolus vulgaris have been examined with respect to the level of gene product produced in the seeds of transgenic tobacco. our studies indicate that several upstream regions quantitatively modulate expression. between -1000 and -675, a negative regulatory element reduces expression approximately threefold relative to shorter deletion mutants that do not contain this region. positive regulatory elements lie bet ... | 1989 | 2535513 |
| regulation of beta-glucuronidase expression in transgenic tobacco plants by an a/t-rich, cis-acting sequence found upstream of a french bean beta-phaseolin gene. | a 0.8-kilobase fragment from the 5'-flanking region of a french bean beta-phaseolin gene yielded strong, temporally regulated, and embryo-specific expression of beta-glucuronidase (gus) in transgenic tobacco plants, paralleling that found for the seed protein phaseolin [sengupta-gopalan, c., reichert, n.a., barker, r.f., hall. t.c., and kemp, j.d. (1985) proc. natl. acad. sci. usa 82, 3320-3324]. gel retardation and footprinting assays using nuclear extracts from immature bean cotyledons reveale ... | 1989 | 2535526 |