| characterization of tumor rejection antigen molecules of chemically induced murine colon tumor c-c26. | the molecular nature of a tumor-specific transplantation antigen (tsta) of a chemically induced balb/c mouse colon tumor c-c26 was investigated. the antigen was noncytolytically extracted by 2.5% n-butanol treatment of the cells. crude butanol extract from c-c26, but not from colon tumor c-c51 and fibrosarcoma meth-a of balb/c mice could provide protection against the challenged c-c26 tumor in the transplantation experiment. crude butanol extract from another syngeneic colon tumor c-c36 also ind ... | 1987 | 3581064 |
| a human sperm coating antigen isolated from sperm cell membrane. | a human sperm cell membrane antigen that is highly specific to sperm and seminal plasma was isolated from plasma membrane fraction of spermatozoa using rabbit antiserum against human seminal plasma. in addition to the high specificity to sperm and seminal plasma, the isolated antigen showed the following characteristics: (1) it is a glycoprotein of approximately 12,000 daltons that has an affinity to lentil lectin; (2) it is distributed in human milk other than in sperm and seminal plasma, but i ... | 1985 | 4037175 |
| alterations in fucosyl oligosaccharides of glycoproteins during rat liver regeneration. | [3h]fucose-labelled glycopeptides in the slices of liver 24h after partial hepatectomy were fractionated on sephadex g-50. glycopeptides from regenerating liver contained a higher proportion of lower-mr components than did controls. regenerating liver contained a higher proportion of glycopeptides that were bound to concanavalin a-sepharose and were subsequently eluted with 20mm-methyl alpha-d-glucopyranoside than did controls. concanavalin a-bound glycopeptides from each source were entirely bo ... | 1985 | 4038278 |
| purification and characterization of a high molecular weight glycoprotein detectable in human milk and breast carcinomas. | the murine monoclonal antibody (mab) designated df3 has defined a high m.w. antigen detectable in human breast carcinomas and in human milk. df3 antigen is detectable on apical borders of secretory mammary epithelial cells and in the cytosol of less differentiated malignant cells. df3 antigen expression has been shown to correlate with the degree of human breast tumor differentiation, and the detection of a cross-reactive species in human milk has suggested that df3 antigen might be useful as a ... | 1985 | 4045199 |
| comparative biochemical analysis of purified s-antigen from human, bovine, porcine and rat retina. | highly purified s-antigen from human, bovine, porcine and rat retina was used to ascertain the molecular weights, isoelectric points, amino-acid composition and presence of carbohydrate moieties. all s-antigen preparations comigrate to 50,000 mw on sds-polyacrylamide gels and demonstrate microheterogeneity upon isoelectric focusing. human s-antigen exhibits two bands at ph 5.9 and 5.6, whilst with bovine s-antigen a broad band at ph 5.9 was observed. porcine and rat s-antigen gave four bands, fo ... | 1987 | 3582507 |
| effects of feeding ascochyta-infected and normal lentils to rats (short-term study). | weanling, female, sprague-dawley rats were fed diets containing either rat chow with no lentils, 80% normal lentils or 80% diseased lentils heavily infected with the fungus ascochyta lentis. body weight, feed consumption and clinical appearance were monitored over 90 days and blood samples were collected at the termination of the experiment. weight gain and feed consumption were similar in the control group and the group fed diseased lentils. weight gain was slightly depressed in the group fed n ... | 1985 | 4075241 |
| purification and characterization of a human tumor necrosis factor from the lukii cell line. | a factor with tumor necrosis factor (tnf) activity produced by the lukii human lymphoblastoid cell line [designated tnf(lukii)] was purified sequentially by using controlled-pore glass, lentil lectin-sepharose, and procion red agarose chromatography, yielding tnf with a specific activity of 1.5 x 10(7) units per mg of protein and an isoelectric point of approximately equal to 6.7. purified tnf(lukii) fractionated by nadodso4/page under reducing as well as nonreducing conditions was found to cont ... | 1985 | 3863119 |
| studies on phytohemagglutinins xv. hemagglutinins of the pea and the lentil: advantage of their application in comparison to concanavalin a in some agglutination reactions. | | 1973 | 4203917 |
| the effects of thrombin on phytohemagglutinin receptor sites in human platelets. | we have previously demonstrated that lentil phytohemagglutinin (lentil-pha) binds to human platelet membranes without causing either aggregation or the release reaction. when platelets are treated with thrombin, there is an increase in lentil-pha binding suggesting the appearance of new receptor sites on the cell surface. we prepared a lentil-pha-ferritin conjugate using affinity chromatography which was used to saturate cell surface receptor sites. studies using this conjugate suggest that thro ... | 1974 | 4207395 |
| the receptor proteins for concanavalin a and lens culinaris phytohemagglutinin in the membrane of the human erythrocyte. | | 1974 | 4211043 |
| purification of virus glycoproteins by affinity chromatography using lens culinaris phytohaemagglutinin. | | 1973 | 4352507 |
| stimulation of guinea pig lymphocytes by lens culinaris lectin-a. stimulation by insolubilized lcl-a and effect of erythrocytes and peritoneal macrophages on stimulation by soluble lcl-a. presence of a lymphokine in culture fluids of lcl-a-stimulated cells. | | 1974 | 4455382 |
| binding of purified lectins to guinea pig lymphocytes. studies of the number, binding constant, and distribution of lens culinaris lectin a and agaricus bisporus lectin molecules on lymphocyte surfaces. | | 1974 | 4615817 |
| studies on the appearance and location of hemagglutinins from a common lentil during the life cycle of the plant. | | 1972 | 4622779 |
| studies on a phytohemagglutinin from the common lentil. vi. stimulation of human peripheral lymphocytes in culture by lens culinaris hemagglutinin a. | | 1972 | 4674698 |
| a method of lentil inoculation with fusarium oxysporum schlecht. | | 1973 | 4740794 |
| studies on a phytohemagglutinin from the lentil. ii. multiple forms of lens culinaris hemagglutinin. | | 1971 | 4993956 |
| studies on phytohemagglutinins. ix. metal content and activity of the hemagglutinin from the lentil (lens esculenta moench). | | 1971 | 5002473 |
| isolation of glycoproteins from pig lymphocyte plasma membrane using lens culinaris phytohemagglutinin. | | 1972 | 5026302 |
| studies on a phytohemagglutinin from the lentil. v. binding of lens culinaris hemagglutinin to lymphocytes and erythrocytes. | | 1972 | 5065140 |
| [mutual effect of some parasitic fungi, rhizobium, and other microorganisms of lens (lens esculenta moench) and the biological possibilities of protection]. | | 1970 | 5537326 |
| the protein efficiency ratio of raw and cooked lentils (lens esculenta) and the supplenentary effect of rice and wheat proteins. | | 1967 | 5605721 |
| pulmonary granulomatosis caused by vegetable particles. so-called lentil pulse pneumonia. | | 1969 | 5812763 |
| effect of methionine, vitamin b12 and hydrolysed glucose cycloacetoacetate on protein nutritive value of lens esculenta (lentil). | | 1965 | 5892224 |
| recognition by a mouse monoclonal antibody of a glycoprotein antigen of rat brain which is expressed intracellularly by neurons. | we describe a previously uncharacterised glycoprotein antigen of rat brain. the antigen was localised by immunofluorescence on 10 micron cryostat tissue sections, and was found to be present intracellularly in neurons. no other cell types or structures within the brain were stained. the antigen is recognised by a mouse monoclonal antibody called ngp41. the antibody was produced after immunising a mouse with glycoproteins purified by lentil lectin affinity chromatography of solubilised rat brain ... | 1984 | 6083506 |
| serological cross-reactivities of murine and human class ii antigen determined by murine xeno anti-human class ii antibody. | murine anti-human class ii antibodies were shown to cross-react with polymorphic determinants of murine class ii antigens. the cross-reacting antibodies were raised in b10.s(9r) mice by immunizing with human nylon wool adherent cells (ad cells) from peripheral blood leukocytes. the b10.s(9r) anti-human ad cell antiserum bound to the molecules consisting of two chains with molecular weights of 35k and 28k dimers which were purified with a lentil-lectin column. the b10.s (9r) anti-human class ii a ... | 1984 | 6084163 |
| induction and characterization of lymphotoxins from tumor promoter-synergized, lectin-stimulated human lymphocytes in vitro. | the tumor promoters mezerein and phorbol myristate acetate, and the phorbol diesters phorbol diacetate, phorbol dibenzoate, and phorbol didecanoate synergistically enhanced the production of lymphotoxin (lt) by phytohemagglutinin-stimulated human peripheral blood or tonsil and adenoid lymphocytes. lt production was elevated 2-20-fold, depending on such parameters as the nature of the promoter and dose, the lectin dose, and the lymphocyte source. the increased lt levels were primarily due to enha ... | 1985 | 3873519 |
| adoptive transfer of experimental allergic encephalomyelitis with lectin-activated spleen cells. | adoptive transfer of experimental allergic encephalomyelitis (eae) was achieved in lewis rats using spleen cells incubated in vitro with various lectins. when stimulated with concanavalin a (con a), its derivative succinyl-con a, lens culinaris or pisum sativum agglutinin, spleen cells obtained from rats immunized with encephalitogenic emulsion could transfer eae to normal syngeneic recipients. in contrast, spleen cells stimulated with phaseolus vulgaris, robinia pseudoacacia or wisteria floribu ... | 1984 | 6085110 |
| immunoaffinity isolation of ductal carcinoma antigen using monoclonal antibody f36/22. | monoclonal antibody (mcab) f36/22, raised against a human breast tumor line, identifies an antigen found in the circulation of cancer patients. antigen was purified from malignant effusions using mcab-affinity chromatography followed by adsorption-desorption from immobilized wheat germ lectin. electrophoretic analysis demonstrated the isolation of a single high mol. wt glycoprotein exhibiting an isoionic point near ph 4.2 and a density of approx. 1.45 g/ml. although highly reactive with wheat ge ... | 1984 | 6095073 |
| biochemical characterization including amino acid and carbohydrate compositions of canine and human brain thy-1 antigen. | human and canine brain thy-1 antigens were solubilized in deoxycholate and antigen activity was followed both by conventional absorbed anti-brain xenosera of proven specificity and by mouse monoclonal antibodies to canine and human thy-1. it is shown that greater than 80% of thy-1 activity in the dog and man binds to lentil lectin, that the mobility on sodium dodecyl sulphate/polyacrylamide-gel electrophoresis of canine and human thy-1 is identical with that of rat thy-1 and that the stokes radi ... | 1981 | 6119980 |
| carbohydrate complexity of the mouse thymocyte thy-1 glycoprotein as demonstrated by lectin affinity and isoelectric focusing. | the thy-1 glycoprotein of mouse thymocytes was analysed with regard to the properties of its carbohydrate part. of the different lectins tested, partial binding of thy-1 from membrane extracts was found to lens culinaris agglutinin (approximately 50%) and wheat germ agglutinin (approximately 25%). in contrast, concanavalin a bound all thy-1. the results indicate that there is considerable microheterogeneity in the oligosaccaride chains within the thy-1 population. thy-1 was purified from thymocy ... | 1982 | 6121705 |
| the effect of h-2ij disparity on induction of allotransplantation tolerance by lentil lectin. | the effect of an h-2ij disparity on skin graft survival was studied in 18 mouse donor-recipient strain combinations, in which the recipients were treated with an efficient immunosuppressant, lentil lectin (lca). the simultaneous i-j disparity essentially had no (or a slightly adverse) effect on the graft survival times in strain combinations differing at the k and i-a loci or in the entire h-2 complex. in two strain combinations incompatible at the d locus, the simultaneous i-j disparity promote ... | 1985 | 3884497 |
| processing of rat and human angiotensinogen precursors by microsomal membranes. | we have studied the processing of rat and human angiotensinogen precursors by microsomal membranes as a means of determining the number of asparagine-linked oligosaccharide units per angiotensinogen molecule, and thus the utilization of potential sites of n-glycosylation. glycosylated, processed forms of angiotensinogen were isolated by chromatography on lentil lectin-sepharose 4b. 35s-methionine-labeled precursor and processed forms of angiotensinogen were compared with glycosylated and nonglyc ... | 1985 | 3934016 |
| partial characterization of the oligosaccharides of mouse thymocyte thy-1 glycoprotein. | four glycopeptides (i, iia, iib, iii) with different oligosaccharide structures were isolated from purified mouse thymocyte thy-1 glycoprotein. the glycoprotein was digested with pronase, and the glycopeptide fraction was isolated by gel filtration and acetylated with [3h]acetic anhydride. the different glycan structures were separated by affinity chromatography on concanavalin a-sepharose 4b and lentil lectin-sepharose 4b. size determinations of intact and exoglycosidase- and endoglycosidase-di ... | 1984 | 6148073 |
| nature of hapten-modified determinants involved in induction of t cell tolerance and suppressor t cells to ndfb contact sensitivity. | unresponsiveness to dnfb contact sensitivity induced by dnp-modified lymphoid cells (dnp-lc) is mediated by two separable pathways: a rapidly induced, long lasting inhibition of reactive t cell clones (donor tolerance), and a transient period of suppressor t cell (ts) activity. the present report has examined the nature of the hapten-modified determinants responsible for the induction of these pathways by utilizing soluble dnp-lc cell lysate preparations as tolerogens. the results indicate that ... | 1980 | 6153673 |
| proteins of rapid axonal transport: polypeptides interacting with the lectin from lens culinaris. | | 1980 | 6154775 |
| interaction of lectins with neisseria gonorrhoeae. | fifty-four strains of neisseria gonorrhoeae were examined for their ability to be agglutinated by various lectins. wheat germ agglutinin, ricin, soybean lectin, and peanut agglutinin agglutinated all strains tested. dolichos biflorus, sophora japonica, maclura pomifera, ulex europaeus, lens culinaris, canavalia ensiformis, phaseolus lunatus, and bandeiraea simplicifolia bs i and bs ii lectins either failed to agglutinate or agglutinated some strains but not others. agglutination of gonococci by ... | 1980 | 6155190 |
| the localization of lectin binding sites on photoreceptor outer segments and pigment epithelium of dystrophic retinas. | carbohydrate-containing macromolecules on pigment epithelium (pe) and photoreceptor outer segment (os) membranes of 14 to 16-day-old royal college of surgeons (rcs) rats and their genetic control (rcs-rdy+) have been localized with peroxidase-conjugated lectins from wheat germ agglutinin (wga), ricinus communis (rca), lens culinaris (lca), and concanavalin a (con a). all lectins stain the plasma membranes of photoreceptor inner segments and intact oss of normal (rcs-rdy+) and dystrophic (rcs) re ... | 1980 | 6156139 |
| creation and properties of histocompatibility antigen-cell conjugates. i. preparation and immunological properties of rat histocompatibility antigens. | a necessary step in the induction of unresponsiveness to transplantation antigens is the preparation and presentation of tissue antigens in a suitable form. a method is presented for obtaining rat histocompatibility antigen in a soluble, "heptenic" form suitable for coupling to a tolerogencie carrier. lewis and aci spleen cell membranes are solubilized with deoxycholate and purified by lens culinaris affinity chromatography. the ultracentrifuged, solubilized preparation of rat histocompatibility ... | 1980 | 6157235 |
| high molecular weight antigens present on human t cells. | a series of eight high molecular weight (140,000-220,000) glycoproteins on human peripheral t cells were recognized by radioimmunoprecipitation with a rabbit antiserum. the pattern of antigens present on each of eight human t cell lines studied was unique, and no line displayed the range of antigens present on peripheral t cells. the pattern of bands on peripheral t cells changed after allogeneic or lectin stimulation. adsorption/elution experiments with antiserum showed that some of these prote ... | 1980 | 6161372 |
| the biochemical characterization of a cell surface antigen associated with acute lymphoblastic leukemia and lymphocyte precursors. | the acute lymphoblastic leukemia- (all) associated membrane antigen is a single glycosylated polypeptide of approximate m.w. of 100,000 (gp100), containing no intrachain disulfide linkages. approximately 50% of gp100 will bind to lentil lectin, whereas 100% will bind to the lectin from ricinus communis. both lentil-binding and lentil nonbinding forms of the antigen appear to be identical by 2-dimensional isoelectric focusing/sds polyacrylamide gel electrophoresis and peptide mapping. carbohydrat ... | 1981 | 6163827 |
| separation and comparison of human tl-like antigens and hla(a, b, c) antigens expressed on cultured t cells. | beta 2-microglobulin-bound t-cell membrane components containing both human tl-like antigens and hla(a, b, c) antigens were partially purified from renex 30-solubilized membrane material of cells of a human t-cell-type leukemia cell line, hpb-all. the radioiodinated preparation was subjected to limited papain digestion; the hla(a, b, c) antigens split, whereas a large portion of the human tl-like antigens remained intact. the antigen molecules were recovered by lentil-lectin affinity chromatogra ... | 1981 | 6164635 |
| glycoproteins in the whorls of membrane produced by oligodendroglia in culture. | two glycoproteins of 99 kda and 77 kda which exhibit intense binding to wheat germ agglutinin have been purified from the whorls of membrane produced by oligodendroglia in culture. the whorls of membrane were isolated by gradient centrifugation from purified bovine oligodendroglia maintained in culture. the two glycoproteins were solubilized from the membranes using a non-ionic detergent and purified by sephadex lh-60 chromatography, wheat germ agglutinin affinity chromatography, and sds-polyacr ... | 1987 | 3651447 |
| the in vitro production of alpha--fetoprotein variants by human fetal organs. | we have demonstrated the immunological identity of alpha-fetoprotein (afp) produced in vitro by various fetal organs such as the yolk sac, liver, intestine and kidney. afp produced in vitro by these organs, present in fetal serum and in samples of amniotic fluid was, however, heterogeneous when studied by lectin affinity immunoelectrophoresis. results obtained with concanavalin a were comparable to those obtained with lentil agglutinin, but concanavalin a separated afp into two variants only, wh ... | 1980 | 6169062 |
| isolation from human chronic-lymphocytic-leukaemia cells of membrane glycoproteins associated with fc-receptor functions. physical parameters and production of polyclonal antibodies. | two membrane glycoproteins that bound immune complexes and inhibited fc-receptor- (fcr-)mediated functions in vitro were purified from human fcr+ chronic-lymphocytic-leukaemia cells. a multi-step purification was developed, consisting essentially in: (i) tween 40 extraction of crude cell membranes; (ii) solubilization of membrane fragments by renex-30; (iii) isolation of glycoproteins by affinity chromatography on lens culinaris haemagglutinin-sepharose; (iv) papain treatment of the eluted glyco ... | 1987 | 3663159 |
| evidence for glycoprotein components of the hepatocellular bile acid transporter. | the hepatocellular transporter, responsible for the uptake of bile acids and some foreign substances, can be shown to contain carbohydrate moieties. the hepatocellular uptake of cholate and phallotoxin is immediately inhibited by addition of wheat-germ agglutinin. concanavalin a and lentil lectin reduce the uptake in a time-dependent manner. apparently sialic acids or n-acetylglucosamine residues are involved in the translocation process. polypeptides (mr 50,000, 54,000) of the above transport s ... | 1987 | 3663657 |
| horseradish peroxidase (hrp) conjugates of cholera toxin and lectins are more sensitive retrogradely transported markers than free hrp. | horseradish peroxidase (hrp) conjugates of 8 different lectins (wheat germ agglutinin, ricinus communis i and ii, peanut agglutinin, lens culinaris, soybean agglutinin, limulus polyhemus, ulex europaeus i) and cholera toxin (ct) or free hrp (fhrp) were individually injected into the submandibular gland (smg) or anterior chamber (ac) of the eye and the retrogradely labeled neurons in the superior cervical ganglion (scg) were quantitated. the effect of using 3 different cross-linking reagents (glu ... | 1982 | 6173093 |
| developmental changes of amniotic fluid alpha-fetoprotein subfractions in early gestation. | using both concanavalin a crossed-line affinity immunoelectrophoresis and lentil lectin crossed-line affinity immunoelectrophoresis, developmental changes of alpha-fetoprotein (afp) subfractions were studied in 11 samples of human amniotic fluid obtained between 43 and 121 days of gestation. in the amniotic fluid at 43 or 48 days of gestation, only subfractions produced by the yolk sac were evident. the initial appearance of the subfractions produced by the fetal liver was first detected at 51 d ... | 1983 | 6190415 |
| application of lectin--gold complexes for electron microscopic localization of glycoconjugates on thin sections. | a method is described for the electron microscopic detection of lectin-binding sites in different cellular compartments and extracellular structures that uses thin sections from resin-embedded tissues. various lectins (ricinus communis lectin i and ii, peanut lectin, lotus tetragonolobus lectin, ulex europeus lectin i, lens culinaris lectin, helix pomatia lectin, and soybean lectin) were bound to particles of colloidal gold and used for direct staining of thin sections or glycoprotein--gold comp ... | 1983 | 6190857 |
| similarities in glycosylation of human neuroblastoma tumors and cell lines. | the presence of fucosyl residues linked alpha 1----3(4) to n-acetylglucosamine was demonstrated on the oligosaccharides from glycoproteins of 11 human neuroblastoma tumors from ten different patients. this finding is in complete agreement with the previous report that human neuroblastoma cell lines contained an unusually large proportion of metabolically incorporated l-[3h]fucose in this specific linkage (u. v. santer and m. c. glick, cancer res., 43:4159-4166, 1983). furthermore, the glycopepti ... | 1986 | 3708596 |
| t cell hybrids that express a vh idiotope-related determinant on a glycoprotein distinct from h-2, thy-1, and lyt-1 molecules. | two mouse monoclonal antibodies to chicken immunoglobulin vh-associated idiotypes (id), cid-1 and cid-2, were used as probes for id determinants on mouse t cells. cid-1, which recognized chicken antibodies to n-acetyl glucosamine (naga), and approximately 0.4% of chicken t lymphocytes also reacted with approximately 0.2% of balb/c splenic thy-1.2+ cells. when enriched cid-1+ splenic t cells from naga-immune balb/c mice were fused with the akr thymoma bw 5147 cell line, 2 of 72 resulting hybrids, ... | 1983 | 6193222 |
| synthesis of a spore-specific surface antigen during sporulation of saccharomyces cerevisiae. | antisera raised against purified yeast ascospores caused agglutination of both ascospores and vegetative cells. a spore-specific activity was obtained by absorbing out anti-vegetative activity with vegetative cells. the anti-vegetative cell activity was directed against mannan, and was probably due to exposure of some spore coat mannan at the spore surface since concanavalin a and lentil lectin also caused agglutination of ascospores. the spore-specific activity was probably determined by a prot ... | 1983 | 6193243 |
| vil-a1, a monoclonal antibody reactive with common acute lymphatic leukemia cells. | the vil-a1 monoclonal antibody raised against reh cells reacts with common acute lymphatic leukemia (call) cells but not with normal or malignant b or t lymphocytes. it also shows no binding to normal or malignant myeloid, monocytic or erythroid cells, nor does it react with thrombocytes. the antibody is of igm class and lyses call cells very efficiently in the presence of rabbit but not human complement. immunoprecipitation experiments followed by sds-polyacrylamide gel electrophoresis under re ... | 1982 | 6193374 |
| presence of an antigenic determinant common to rat ige-potentiating factor, ige-suppressive factor, and fc epsilon receptors on t and b lymphocytes. | polyclonal antibodies against purified ige-potentiating factor were prepared in guinea pigs, and mouse monoclonal antibodies were prepared against inactive ige-binding factor from a t cell hybridoma. the polyclonal antibodies and two of the monoclonal antibodies bound the ige-potentiating factor, the ige suppressive factor, and the inactive ige-binding factor. the results indicate that the three ige-binding factor molecules share a common antigenic determinant. the polyclonal antibodies and the ... | 1984 | 6197453 |
| characterization of the antigen (gp600) of heymann nephritis. | the nephritogenic brush border glycoprotein antigen (gp600) of autoimmune membranous glomerulonephropathy (heymann nephritis) isolated from crude proximal renal tubular membrane fraction (fx1a) by lens culinaris lectin affinity chromatography was characterized. the molecular weight of the antigen determined by molecular exclusion chromatography was found to be 600,000. on sodium dodecyl sulfate-polyacrylamide gel electrophoresis it resolved into five polypeptides which were in the molecular weig ... | 1984 | 6199583 |
| distinct molecular species of human alpha-fetoprotein due to differential affinities to lectins. | resolution of human alpha-fetoprotein (afp) into four distinct molecular species was demonstrated by a combination of two affinity chromatographies with crossed-immuno-affino-electrophoresis (ciae) using concanavalin a (con a) and lens culinaris hemagglutinin (lch)-a and lch-b as affinity media. of the four afps, afp1 had no affinity for con a, lch-a, or lch-b; afp2 showed a high affinity for con a, a low affinity for lch-a, and an intermediate affinity for lch-b (or a low affinity, depending on ... | 1983 | 6200049 |
| a rapid procedure for the enrichment of undenaturated, antigenically active herpes simplex virus glycoproteins. | the usefulness of lentil lectin affinity chromatography for the rapid enrichment of hsv glycoproteins in an undenatured state for both research and clinical purposes was investigated. in order to compare the lentil lectin-binding characteristics and immunologic specificities of undenatured hsv-1 and hsv-2 glycoproteins, [35s]methionine-labelled extracts of virus-infected hep-2 cells were subjected to lentil lectin affinity chromatography. individual hsv-1 and hsv-2 glycoproteins in bound and unb ... | 1984 | 6200489 |
| cell surface glycoproteins of rabbit lymphocytes: characterization with monoclonal antibodies. | the structural characteristics of antigens recognized by a panel of monoclonal antibodies prepared against a rabbit t-lymphocyte cell line have been investigated. those antigens which could be isolated using immunoadsorbents prepared from the monoclonal antibodies had mol. wts of 42,000, 90,000 and 120,000. the 42,000 mol. wt molecule is similar or identical to a rabbit class i major histocompatibility complex antigen and its characterization has been reported elsewhere. three different 90,000 m ... | 1984 | 6200770 |
| studies on lectin affinities of chicken alpha-fetoprotein during ontogeny. | alpha-fetoprotein in the sera of chicken embryos (ch-afp) of the 7th, 10th, 13th, 16th, and 19th day of incubation and of one-day-old chickens were examined in crossed-immunoaffino-electrophoresis (ciae) using different lectins [concanavalin a (con a), lentil, wheat germ lectin, and soybean lectin] as ligands. differences in lectin affinities, suggesting different degrees or forms of glycosylation of ch-afp during ontogeny, were demonstrated by this method. sera of chicken embryos of the same st ... | 1984 | 6200871 |
| interferon induction in mouse spleen cells by mitogenic and nonmitogenic lectins. | twenty-two species of lectin were tested for their ability to induce interferon (ifn) in mouse spleen cells. twenty-two species of lectins representing four groups, based on competition patterns with monosaccharides, were examined for their ability to induce ifn in cultured mouse spleen cells. the lectins, all belonging to the third group (concanavalin a, succinylated concanavalin a, lens culinaris lectins type a and b, and poke weed mitogen) induced ifn mainly composed of ifn gamma. they were e ... | 1984 | 6201544 |
| purification and characterization of rana pipiens brain thy-1 glycoprotein. | the occurrence of thy-1 antigens in rana brain has been studied by the use of heterologous anti-rana brain antisera raised in rabbit and balb/c mouse (thy-1.2) and akr/j mouse (thy-1.1) strains and by monoclonal anti-mouse thy-1.1 and anti-mouse thy-1.2 antibodies with the use of quantitative absorption assays. three antigenic determinants were defined on rana brain and referred to as: 1) the rana-specific xenoantigen, 2) the rana-mouse cross-reacting xenoantigen, and 3) the thy-1.1 antigen. thy ... | 1984 | 6201547 |
| a comparison of histamine secretion from peritoneal mast cells of the rat and hamster. | functional mast cells have been obtained by peritoneal lavage of the rat and hamster. both cell types released histamine on stimulation with appropriate dilutions of anti-rat ige and anti-hamster serum. the maximum response evoked by each reagent was significantly greater for the hamster cells. the release was non-cytotoxic and was in each case blocked by the corresponding soluble antigen. the rat and hamster cells responded to concanavalin a and the lectin from lentil. phosphatidylserine (ps) p ... | 1984 | 6202354 |
| alpha-fetoprotein subfractions in germ cell tumors identified by con a or lch crossed-line affinity immunoelectrophoresis. | using concanavalin a (con a) crossed-line affinity immunoelectrophoresis and lentil lectin (lch) crossed-line affinity immunoelectrophoresis, alpha-fetoprotein (afp) subfractions were studied in sera including three sera from nude mice heterotransplanted with human yolk sac tumor of the ovary and three sera from patients with yolk sac tumor, mature solid teratoma, or immature solid teratoma of the ovary. in sera of nude mice bearing yolk sac tumor or from a patient with yolk sac tumor, subfracti ... | 1984 | 6204543 |
| production and characterization of monoclonal antibody to a melanoma specific glycoprotein. | an immunogen consisting of a 4m urea extract derived from human melanoma cells (m14), that was devoid of hla-a,b,c, hla-dr antigens and fibronectin was adsorbed to lens culinaris lectin-sepharose 4b and used to immunize mice for production of monoclonal antibody to a melanoma-specific glycoprotein. screening for hybridomas secreting antibodies to melanoma associated antigens was facilitated by use of a solid phase target antigen of chemically defined medium of melanoma cells (cdm). use of these ... | 1981 | 6208119 |
| lymphocyte recognition of lymph node high endothelium. vi. evidence of distinct structures mediating binding to high endothelial cells of lymph nodes and peyer's patches. | lymphocytes migrate from blood into lymph nodes (ln) and peyer's patches (pp) of rats specifically at segments of venules lined by high endothelium (hev). we previously identified and isolated a lymphocyte surface component termed high endothelial binding factor (hebf) that appears to be involved in lymphocyte adhesion to high endothelial cells of ln. hebf has also been isolated from thoracic duct lymph and is antigenically related to the cell surface component. soluble hebf derived from deterge ... | 1984 | 6208268 |
| expression of cell surface lectins on activated human lymphoid cells. | a cell surface lectin found on activated human lymphoid cells has been identified and characterized using membrane glycoprotein micelles as probes. these micelles, which are large, water-soluble aggregates, are composed of glycoproteins isolated from detergent-solubilized membranes of human b lymphoblastoid cell lines by lens culinaris hemagglutinin affinity chromatography. the micelles have an average apparent molecular weight of 4 x 10(6) estimated by gel filtration and range in diameter from ... | 1982 | 6214410 |
| ige-binding factors from mouse t lymphocytes. i. formation of ige-binding factors by stimulation with homologous ige and interferon. | incubation of normal mouse spleen cells with homologous ige resulted in the formation of soluble factors that inhibited rosette formation of mouse fc epsilon r+ cells with ige-coated ox erythrocytes. the soluble factors could be absorbed with mouse or rat ige coupled to sepharose and recovered from the beads by acid elution. however, the factors had no affinity for either human ige or mouse igg. the ige-binding factors were derived from t cells. production of the factors required lyt1+ t cells a ... | 1983 | 6217246 |
| comparisons between true digestibility of total nitrogen and limiting amino acids in vegetable proteins fed to rats. | values (%) for true digestibility (td) of protein and individual amino acids in some vegetable proteins were determined by the rat balance (fecal) method. diets containing 8% crude protein (n x 6.25) from soaked and autoclaved samples of trapper and century field peas, lentil, pinto bean, seafarer bean, black bean or fababean and autoclaved samples of soybean, peanut, sunflower, rolled oat, rice + soybean and corn + pea were tested in two rat balance studies. in the case of blends, each protein ... | 1986 | 3746456 |
| modulation of the biologic activities of ige-binding factors. iii. switching of a t cell hybrid clone from the formation of ige-suppressive factor to the formation of ige-potentiating factor. | incubation of rat-mouse t cell hybridoma cells, 23b6, with rat immunoglobulin e (ige) results in the formation of the 15,000-dalton ige-suppressive factor and the 30,000-dalton ige-binding factor, which has neither potentiating activity nor suppressive activity on the ige response. another t cell hybridoma, 23a4 cells, produces the 30,000-dalton "inactive" ige-binding factor upon incubation with ige. both the 15,000-dalton ige-suppressive factor and the 30,000-dalton ige-binding factor lacked af ... | 1983 | 6224847 |
| biphasic changes of the immunological reactivity in the course of experimental lectin-induced arthritis of rabbits. | a single injection of lens culinaris lectin (lcl) into the knee joint cavity of non-sensitized rabbits produces an arthritis with an acute and chronic phase, lasting up to one year. the persistence of the lectin in the joint, related to the strong binding affinity of lectins to glycoproteins of connective tissue structures, and the presence of specific antibodies against lcl in the serum after the intra-articular injection make this model comparable to the antigen-induced arthritis. but in our s ... | 1983 | 6227494 |
| interaction of human platelet membrane glycoproteins with collagen and lectins. | the binding of platelets to collagen is the first step in hemostasis. we attempted three approaches for elucidation of the chemical nature of receptors of human platelets for collagen. first, we examined the effect of platelet surface alteration by chymotrypsin treatment. on increasing the concentration of chymotrypsin, collagen-induced platelet aggregation and the release reaction decreased, an in parallel with this change, remarkable decrease of membrane glycoproteins iib and v, as well as 400 ... | 1984 | 6229286 |
| fractionation and characterization of allergens extracted from eggs of schistosoma japonicum. | allergenic components were partially purified from the crude extract of eggs of schistosoma japonicum by gel filtration on sephadex g-200 and ion-exchange chromatography on deae-sephadex a-50. allergenic activity was assayed by the prausnitz-küstner type skin test with infected mouse sera and radioallergosorbent test with human ser. s. japonicum egg allergens (jeal) showed single and three allergenic peaks, by isoelectrofocusing (pi = 4.8) and polyacrylamide gel electrophoresis, respectively. th ... | 1981 | 6257614 |
| characterization of human platelet surface and intracellular membranes isolated by free flow electrophoresis. | high voltage free flow electrophoresis has been applied to the separation of human platelet membranes. after short treatment with neuraminidase at the whole cell level, three membrane vesicle subpopulations have been isolated. using a surface label (125i-labeled lens culinaris lectin), the marker enzyme nadh-cytochrome c reductase, and lipid analysis, two of the fractions have been identified as of surface origin and the other consists of intracellular membrane elements. the distribution of aden ... | 1981 | 6260785 |
| glycoprotein characteristics of the sodium channel saxitoxin-binding component from mammalian sarcolemma. | the saxitoxin-binding component of the excitable membrane sodium channel exhibits glycoprotein characteristics as evidenced by its specific interaction with various agarose-immobilized lectins. the detergent-solubilized saxitoxin-binding component interacts quantitatively with immobilized wheat germ agglutinin and concanavalin a and fractionally with immobilized lens culinaris hemagglutinin and ricinus communis agglutinin. these lectins preferentially bind n-acetylglucosamine and sialic acid (wh ... | 1981 | 6268157 |
| extracellular folate deaminase of dictyostelium discoideum. | folate deaminase released from cells of dictyostelium discoideum is heterogeneous with respect to molecular weight and stability at 60 degrees c. the most heat-stable component isoelectrofocuses in a broad band at approx. ph 6. the km value of this component for folate is approx. 7 x 10(-7)m and mr approx. 40 000. the major portion if not all of the deaminase binds to immobilized concanavalin a and lentil lectin. extracellular folate deaminase has a ph-optimum of approx. ph 6.0. this is higher t ... | 1981 | 6271253 |
| alkaline phosphodiesterase i and alkaline phosphatase i in plasma membranes of herpes simplex virus type 1 transformed hamster cells. | plasma membrane extracts from herpes simplex virus type 1 transformed hamster embryo fibroblasts were chromatographed on lens culinaris lectin coupled to sepharose (lch-sepharose) and analysed by dodecyl sulphate polyacrylamide gel electrophoresis. coomassie blue-staining revealed two major protein bands with apparent molecular weights of 125 000 and of about 75 000-90 000. in plasma membranes isolated from these tumor cells prior labeled with [3h]fucose or [3h]glucosamine these bands contained ... | 1982 | 6277377 |
| lectin affinity chromatography of sindbis and rous sarcoma virus glycopeptides and oligosaccharides. | glycopeptides and endogly cosidase-digested oligosaccharides from [3h]mannose-labeled rous sarcoma virus and sinbis virus have been fractionated by lentil lectin-sepharose and concanavalin a-agarose affinity chromatography and subsequently analyzed by biogel p-4 gel filtration. only a specific subset of the con a-bound asparaginly-oligosaccharides from he two viruses was also bound to lentil lectin, and this freaction apparently represented fucose-containing, diantennary acidic-type structures ( ... | 1982 | 6286708 |
| redistribution of membrane 5'-nucleotidase in rabbit peritoneal polymorphonuclear leucocytes during phagocytosis. | using a quantitative phagocytic model involving oil droplet internalisation rabbit polymorphonuclear leucocytes display selective segregation of membrane constituents during the phagocytic event. a resting cell surface membrane and fractions representing vesicle membranes and uninvolved surface membranes from the active cells have been purified by density gradient sedimentation and free flow electrophoresis. the specific activity of 5'-nucleotidase, a major neutrophil surface membrane glycoprote ... | 1983 | 6303859 |
| alteration by concanavalin a of the slow dissociable component in the human growth hormone-receptor interaction. | in mice liver plasma membranes (pm), the binding affinity of receptors for [125i] human growth hormone (hgh) was dependent on the association time: after 18 hours, a high affinity receptor form with ka = 6.8 x 10(9) m-1 accumulated and, as compared to after 1 hour, an increase up to 88%, in a slow dissociating component was observed. preincubation of pm with concanavalin a (con a) or other lectins from lens culinaris (lca), ricinus communis (rca i), wheat germ agglutinin (wga) specifically inhib ... | 1986 | 3758923 |
| characterization of the carbohydrate moiety of clerodendron trichotomum lectins. its structure and reactivity toward plant lectins. | lectins were isolated from fruits and leaves of clerodendron trichotomum by affinity chromatography on lactamyl-sepharose. the purified lectins (c. trichotomum agglutinin: cta) were homogeneous on sds/polyacrylamide gel electrophoresis, and the carbohydrate moiety was characterized by physicochemical and immunochemical methods. the asparagine-linked oligosaccharides were released by treatment with n-oligosaccharide glycopeptidase (almond, ec 3.5.1.52) of peptic glycopeptides obtained from fruit ... | 1986 | 3792315 |
| preparation of a highly purified surface membrane fraction from rabbit polymorphonuclear leucocytes by high-voltage free-flow electrophoresis. | a surface membrane fraction of high purity and good yield has been prepared from homogenates of rabbit peritoneal polymorphonuclear leucocytes, using a preliminary sorbitol density gradient sedimentation followed by preparative high voltage electrophoresis in a thin flowing buffer film. enrichment values for the plasma membrane marker enzyme 5'-nucleotidase and 125i-labelled lens culinaris lectin, after the latter had been applied at the whole cell level, were 18-fold and 6-fold, respectively. c ... | 1983 | 6309225 |
| isolation of equine infectious anemia virus glycoproteins. lectin affinity chromatography procedures for high avidity glycoproteins. | lectin affinity chromatography procedures were evaluated for the isolation of enveloped virus glycoproteins. the major glycoprotein of equine infectious anemia virus (eiav) bound to concanavalin a (con a)-sepharose through interactions which could not be reversed by alpha-methylglucoside, but elution could be accomplished with buffers containing guanidine hydrochloride or sodium dodecyl sulfate. these denaturants, however, also released about one-half of the con a protein from the sepharose matr ... | 1983 | 6309879 |
| reaction of beef plasma and lentil seedlings cu-amine oxidases with phenylhydrazine. | the reaction of copper amine oxidases from beef plasma and lentil seedlings with hydrazine derivatives has been studied. a 1:1 stoichiometry was always found for the irreversible binding to the dimeric proteins. the formation of the adduct does not require the presence of oxygen or copper. substrates compete with hydrazine derivatives for the binding to the enzymes. the binding of hydrazines and of substrate has different effects on the epr spectra of enzymic copper. | 1983 | 6312997 |
| solubilization and partial purification of putative calcium channels labelled with [3h]-nimodipine. | high-affinity binding sites for the potent 1,4-dihydropyridine calcium channel blocker [3h]-nimodipine were solubilized from guinea-pig skeletal muscle microsomes with digitonin and chaps [3-(3-cholamidopropyl)-dimethyl-ammonio-l-propanesulfonate]. detergent-solubilized binding sites could not be sedimented by centrifugation (50,000 x g, 4 h), passed freely through 0.2 micron nitrocellulose filters and were stable at 4 degrees c with half-lives of greater than 60 h. the solubilized 1,4-dihydropy ... | 1983 | 6314149 |
| unusual heterogeneity in the glycosylation of the g protein of the hazelhurst strain of vesicular stomatitis virus. | the asparagine-linked oligosaccharides of the g protein of the hazelhurst subtype of the new jersey serotype of vesicular stomatitis virus (vsv) have been compared with the oligosaccharides from the g protein of the well-characterized indiana serotype of vsv, with baby hamster kidney cells in monolayer culture as the host for both viruses. [3h]glucosamine- and [3h]mannose-labeled glycopeptides from the g protein of purified virus were analyzed by the combined techniques of endo-beta-n-acetylgluc ... | 1983 | 6314902 |
| effects of horse and fetal calf serum on the expression of tumor-associated antigen and tumorigenicity of l5178y leukemia/lymphoma cells. | a tumor antigen (ta) associated with murine leukemia-lymphoma l5178y cells has been identified by the enzyme-linked immunosorbent assay (elisa) and indirect immunofluorescence (iif) techniques. the antigen was present in both non-solubilized and 0.5% np-40 solubilized membrane extracts. rabbit anti-l5178y lymphoma serum (rals), extensively absorbed with normal mouse tissues, identified ta in extracts of l5178y lymphoma and l5178y leukemia cells grown in horse serum (l5178y/hs), but not in extrac ... | 1986 | 3796035 |
| glycoprotein gp118 of varicella-zoster virus: purification by serial affinity chromatography. | glycoprotein gp118, one of the major glycosylated proteins specified by varicella-zoster virus, is biologically of great importance since it possesses an epitope which elicits a complement-independent neutralizing antibody response. to purify this glycoprotein from a nonidet-solubilized extract of varicella-zoster virus-infected cells, we examined its affinity to a variety of ligands, including two lectins--concanavalin a and lens culinaris, cibacron blue and heparin, and finally an immunoadsorb ... | 1984 | 6321779 |
| the evaluation of a lectin-agarose based subunit vaccine and complementary diagnostic antigen for aujeszky's disease (pseudorabies) in the pig. | eleven out of 25 pigs were immunized with a lectin--agarose based subunit vaccine for aujeszky's disease (ad). the vaccine was prepared by extracting protective antigens from a non-ionic detergent (triton-x-100) extract of ad virus-infected pk-la cells with lens culinaris agglutinin immobilized on agarose beads. two groups of 3 and 4 pigs received 2 doses of vaccine each containing 426 micrograms of adsorbed protein. two groups of 2 pigs each received 2 vaccine doses containing either 23 or 33 m ... | 1984 | 6326377 |
| a structural analysis of the carbohydrate side chains on class i and class ii histocompatibility antigens of the swine facilitated by heteroantisera specific for the denatured polypeptides. | rabbit heteroantisera specific for the denatured glycoprotein subunits of swine class i and class ii major histocompatibility complex (mhc) antigens have been prepared and utilized to monitor changes in the mobilities of these polypeptides on sds-polyacrylamide gel electrophoresis subsequent to various deglycosylation procedures. this information, in combination with lectin reactivity patterns for the glycoproteins bound to nitrocellulose, has made it possible to define specific structural featu ... | 1986 | 3796628 |
| hepatocyte adhesion to collagen. isolation of membrane glycoproteins involved in adhesion to collagen. | adhesion of hepatocytes to collagenous substrates and their spreading have been shown to involve a specific recognition event, possibly mediated by membrane proteins with affinity for collagen. in the present communication, we describe the isolation of membrane components that are involved in the adhesion of rat hepatocytes to collagen. these components could be solubilized from liver microsomal membranes by treatment with detergents or papain--but not by treatment with edta, urea or high salt. ... | 1986 | 3956590 |
| two distinct antigenic markers for rat thymus and t cells defined by monoclonal antibodies. | rat thymus and t-cell antigens were defined by using two distinct monoclonal antibodies (r-1-3b3 and r-1-12c5). r-1-3b3 antibody, when tested for its reactivity with rat lymphoid cells by immunofluorescence, labelled virtually all thymus and t cells but not b cells and bone marrow cells. the antigen defined by this r-1-3b3 antibody occurred more abundantly on medullary thymocytes and peripheral t cells than on cortical thymocytes. immunochemical data showed that r-1-3b3 antibody recognized a sin ... | 1983 | 6339377 |
| lectin receptors in trypanosoma cruzi. an n-acetyl-d-glucosamine-containing surface glycoprotein specific for the trypomastigote stage. | we have investigated the interaction of three lectins, differing in their sugar specificities, with the surface of the three differentiation stages of trypanosoma cruzi. the scatchard constants for each lectin and parasite stage imply that differentiation of t. cruzi is accompanied by changes in the cell surface saccharides. trypomastigotes obtained from two different sources do not differ appreciably as to the number and affinity of binding sites for the three lectins employed, suggesting a sim ... | 1983 | 6340740 |
| adult transplantation tolerance induced by lentil lectin. iii. induction of transplantation tolerance by lentil lectin in mouse strain combinations with different h-2 disparities: tolerogenic effect of h-2d region antigens. | the survival time of skin allografts was investigated in 28 combinations of mouse strains differing at various loci, in adult recipients treated with lentil seed lectin (lca). the recipients were given 1 mg of lca daily (i.v. or i.p.) after transplantation of the skin graft. the immunosuppressive effect of lca was generally inversely related to the conventional strength of the allotransplantation reaction, i.e. the rate of rejection of allografts on untreated recipients. in the case of h-2d anti ... | 1983 | 6343508 |
| expression of lectin-binding surface glycoproteins during the development of schistosoma mansoni schistosomula. | tegumental glycoproteins of schistosoma mansoni cercariae, mechanically produced 24-hr and 48-hr schistosomula, and adult worms were radioiodinated with the bolton-hunter reagent, then isolated by lectin affinity chromatography. sds-page revealed con a binding glycoproteins with apparent molecular weights of 180,000, 150,000, 43,000, and 30,000 in detergent extracts of the tegument of cercariae. these glycoproteins are retained by 24-hr mechanically produced, cultured schistosomula and are accom ... | 1986 | 3819968 |
| the mechanism of natural binding of bacteria to human lymphocyte subpopulations. | some bacteria bind naturally to human lymphocyte subpopulations. here we investigated the nature of the interacting factors" on bacterial cells and on lymphocytes. first, surface ig was not involved in the binding of b. melitensis to b cells, since capping of surface ig did not affect the binding of this bacteria. we put forward the hypothesis that the binding is due to an interaction between a protein (probably a lectin) on the lymphocyte surface and a carbohydrate on the bacteria. the followin ... | 1983 | 6348298 |
| binding of mammalian beta 2-microglobulin by glycoproteins in fish serum. | the results demonstrate the presence in cod serum of beta 2-microglobulin (beta 2m)-binding molecules. upon fractionation on sephadex g-200, the bound beta 2m appears mainly in the void volume, but also as a minor peak with the apparent size of albumin. the complexes show affinity to con a-sepharose and lens culinaris lectin-sepharose, respectively, indicating that they contain glycoproteins. because of the high molecular weight of the beta 2m-containing complexes they can be separated from unbo ... | 1983 | 6353206 |
| insulin-stimulated phosphorylation of the insulin receptor precursor. | the alpha and beta subunits of the insulin receptor, mr = 135k and 95k, appear to be synthesized via a single polypeptide precursor of mr = 190k. we have investigated whether insulin stimulates the phosphorylation of this proreceptor, as is the case with mature receptor. rat liver endoplasmic reticulum membranes were solubilized in triton x-100 and chromatographed sequentially on wheat-germ agglutinin-agarose and lentil lectin-agarose columns. phosphorylation of the lentil eluate with [gamma 32p ... | 1983 | 6360168 |
| fetal forms of oligoaminopeptidase, dipeptidylaminopeptidase iv, and sucrase in human intestine and meconium. | particles of meconium sedimenting at 105,000 g contain sucrase and various brush border peptidase activities. oligoaminopeptidase, dipeptidylaminopeptidase, and sucrase solubilized by papain from meconium particles of preterm newborns or from brush border of human fetuses during the 4th month of gestation were compared with the same enzymes prepared from adult jejunal and ileal brush border. the following are characteristics of fetal intestinal brush border enzymes: (a) a faster anodal electroph ... | 1984 | 6363665 |
| characterization of a membrane antigen from rabbit testis and sperm isolated by using monoclonal antibodies and effect of its antiserum on fertility. | an antigen was isolated from deoxycholate-solubilized rabbit testis and sperm by using an immunosorbent column containing igg from a monoclonal antibody (8c10.5) that inhibits fertility. elution was by stepwise increases in ph (8.0, 10.0, and 11.4), with the ph 11.4 fraction after recycling through the column showing a single band at 63 kilodaltons in slab nadodso4/page with a silver stain. the antigen molecule was composed of two subunits, which on two-dimensional page showed many spots within ... | 1984 | 6366792 |
| assessment of lymphocyte function during vitamin a deficiency. | two variables of immune status were measured in vitamin a-deficient (a-), vitamin a-sufficient pair-fed (a + pf), and healthy control (a+) rats to examine the mechanisms of immune regulation by vitamin a. parallel samples were run in bovine fetal serum (bfs)-containing medium and serum-free medium to control bfs-origin vitamin a in the cultures. splenocytes derived from a- rats showed significantly depressed blastogenic response to all lectins tested in both mediums when compared with responses ... | 1986 | 3963587 |