| effect of raw legume diets on intestinal absorption of d-galactose by chick. | the effect of four raw legume diets on the intestinal absorption of d-galactose and oxygen consumption were studied in chick. field beans (vicia faba), soybeans (glycine soja), bitter vetch (vicia ervilia), and navy beans (phaseolus vulgaris), were used. the intestinal absorption was determined by both in vivo and in vitro techniques. in vivo, only navy beans and soybeans inhibit intestinal transport of d-galactose, while in vitro all the diets do. oxygen consumption by intestinal rings increase ... | 1980 | 7394275 |
| response of young chicks to raw, defatted, kunitz trypsin inhibitor variant soybeans as sources of dietary protein. | two trials, using 264 8-day-old male crossbred chicks, were conducted to evaluate the nutritional value of two trypsin-inhibitor (kunitz) variant soybeans. these two variants, designated pi 157440 (pi) and number 661, have lower trypsin-inhibitor activities than do us commercially grown soybean cultivars such as amsoy 71. raw pi, 661, and amsoy 71 soybeans were defatted and then compared to a commercially processed solvent extracted soybean meal (sbm) using purified diets with soybeans or sbm as ... | 1980 | 7413562 |
| pollutants in breast milk of vegetarians. | in the article by rogan et al. on pollutants in breast milk, the statement that "there are no obvious...dietary predictors" of chemical pollutants in human milk is challenged by recent unpublished data on vegetarian women. we studied 12 woman who were all members of a religious community that eliminates all animal products from the diet and uses soybeans and grains for proteins. samples of breast milk were gathered in august 1979 and were analyzed for 17 chemical substances. the lipid levels ... | 1981 | 7464895 |
| increase of blood group a and loss of blood group sda activity in the mucus from human neoplastic colon. | aqueous extracts of human neoplastic and adjacent normal colonic mucosa were investigated for hemagglutination inhibition of both the anti-a serum and the dolichos biflorus lectin. the anti-a antiserum was inhibited by the extracts from neoplastic tissues to a much higher extent than by that from normal mucosa; the inhibition was strictly dependent on the blood group and the secretor status of the individual. the inhibition titers against the dolichos lectin were much lower for the tumor than fo ... | 1980 | 7466135 |
| interaction of native and asialo rat sublingual glycoproteins with lectins. | the binding properties of the rat sublingual glycoprotein (rsl) and its asialo product with lectins were characterized by quantitative precipitin(qpa) and precipitin inhibition(qpia) assays. among twenty lectins tested for qpa, native rsl reacted well only with artocarpus integrifolia (jacalin), but weakly or not at all with the other lectins. however, its asialo product (asialo-rsl) reacted strongly with many gal and galnac specific lectins-it bound best to three of the galnac alpha 1-->ser/thr ... | 1995 | 7475931 |
| selective inhibition of bitter taste of various drugs by lipoprotein. | previously, we demonstrated that lipoprotein composed of phosphatidic acid (pa) and beta-lactoglobulin (lg) selectively and reversibly suppress the frog taste nerve response to bitter substances. in the present study, we examined the effects of various lipoproteins on the taste sensation to various stimuli in humans by a psychophysical method. among various lipoproteins composed of different of lipids and proteins, the lipoproteins composed of pa and proteins were most effective in suppressing b ... | 1995 | 7479549 |
| soybean trypsin inhibitor(s) reduce absorption of exogenous and increase loss of endogenous protein in miniature pigs. | it was the purpose of this study to define whether trypsin inhibitors impair protein digestibility via enhanced loss of exogenous or endogenous protein by quantifying those losses using the homoarginine technique, recently developed in this laboratory. pigs fitted with permanent ileal t-cannulas were fed test meals containing homoarginine-labeled protein. the meals contained casein and increasing doses of trypsin inhibitors (experiment 1) or alternatively either heat-treated or raw ground soybea ... | 1993 | 7505318 |
| pancreatic enlargement is evident in rats fed diets containing raw soybeans (glycine max) or cowpeas (vigna unguiculata) for 800 days but not in those fed diets based on kidney beans (phaseolus vulgaris) or lupinseed (lupinus angustifolius). | pancreatic weights and composition were studied with rats fed diets containing raw legume seeds for up to 800 d. rapid pancreatic enlargement was induced by dietary soybeans (glycine max) (high kunitz and bowman-birk trypsin inhibitor contents, moderate lectin content) during the initial 150 d. over the next 200 d the rate of pancreatic growth was similar to that in controls. after 350 d a second period of rapid pancreatic growth occurred. macroscopic pancreatic nodules were evident in a number ... | 1993 | 7505319 |
| mutagenic effects of nitropyrenes in a soybean test system. | the mutagenic activity of 1-nitropyrene (1-np), 1,3-dinitropyrene (1,3-dnp), 1,6-dinitropyrene (1,6-dnp) and 1,8-dinitropyrene (1,8-dnp) was assayed in heterozygous soybean plants (y11y11), based on the appearance of mutational spots (yellow, dark green and twin) on the leaves. 1-np, 1,3-dnp, 1,6-dnp and 1,8-dnp were direct-acting mutagens in a soybean test system, and mutagenicity was enhanced by addition of pyrene as a precursor. the mutagenicity of dinitropyrenes was enhanced by pretreatment ... | 1994 | 7506387 |
| echinostoma caproni and e. trivolvis alter the binding of glycoconjugates in the intestinal mucosa of c3h mice as determined by lectin histochemistry. | mouse (c3h) mucosal glycoconjugates were examined in normal small intestines and intestines infected with echinostoma caproni or e. trivolvis using six different fluorescein-conjugated lectins: triticum vulgaris agglutinin (wga), ulex europaeus agglutinin i (uea-i), ricinus communis agglutinin i (rca-i), glycine max soybean agglutinin (sba), dolichos biflorus agglutinin (dba), and arachis hypogaea peanut agglutinin (pna). the expression of lectin-binding sites and the intensity of the binding of ... | 1993 | 7507137 |
| accumulation of reactive oxygen species and oxidation of cytokinin in germinating soybean seeds. | seed germination is an important developmental switch when quiescent seed cells initiate oxidative phosphorylation for further development and differentiation. during early imbibition of soybean seeds (glycine max l. cv. weber), a superoxide dismutase (sod) activity peak was observed, in embryonic axes, after 6 h imbibition. peroxidase activities, including catalase, were significantly increased after 12 h inhibition and during germination phase iii. catalase was the most efficient enzyme in cat ... | 1994 | 7521301 |
| [natural proteinase inhibitors as a basis for creating new drugs]. | isolation of the proteinases inhibitors, available for medicinal purposes, was described, where the inhibitor of the kunitz type was obtained from bovine pancreas and the inhibitor of the bowman-birk type from soybeans. screening of the immobilization procedures was carried out, which enabled the authors to produce the polymeric conjugates of the proteinase inhibitors exhibiting the maximal rate of activity against pancreatic proteinases and granulocyte elastases. pharmacokinetics of the protein ... | 1994 | 7521547 |
| differential localization of lectin binding sites and neuropeptides in human dorsal root ganglia. | the subpopulations were compared of neurons in human dorsal root ganglia (drg), as substance p, identified by somatostatin, glycine max lectin (sba) specific to terminal n-acetylgalactosamine, and ulex europaeus i agglutinin (uea-i) specific to l-fucose. the lectins and neuropeptides all bound to neurons of small diameter. furthermore, the majority of the sba binding neurons or somatostatin positive neurons were also uea-i binding neurons. however, sba binding neurons were not colocalized with s ... | 1994 | 7531191 |
| bradyrhizobium liaoningense sp. nov., isolated from the root nodules of soybeans. | seventeen strains of extra-slowly growing (esg) soybean rhizobia isolated from root nodules of glycine soja and glycine max growing in five provinces (liaoning, heilongjiang, shanxi, hubei, and anhui) in the people's republic of china were compared with 48 reference strains belonging to the genera bradyrhizobium, rhizobium, and agrobacterium by performing a numerical analysis of 191 phenotypic features. our results showed that all of the esg strains examined clustered closely in the genus bradyr ... | 1995 | 7547289 |
| glycans with n-acetyllactosamine type 2-like residues covering adult schistosoma mansoni, and glycomimesis as a putative mechanism of immune evasion. | glycans at the surface of adult schistosoma mansoni were investigated with gold-labelled lectins. the fragile complex of the glycans with the outer membranes could be preserved for electron microscopy by avoiding extensive pre-fixation with aldehydes and by introducing osmium-ferrocyanide as a membrane fixative. male and female worms were entirely covered with glycans that intensely bound lectins from erythrina cristagalli and datura stramonium, suggesting that galactose(beta 1-4)n-acetylglucosa ... | 1995 | 7567100 |
| deantigenation of human type b erythrocytes with glycine max alpha-d-galactosidase. | conversion of erythrocyte membrane b antigen to h antigen produces blood type o which is universally transfusable. if efficient large-scale production of enzymatically converted red blood cells is to be achieved, then optimal conditions for deantigenation must be determined. cell suspension assays were used to study the blood group b activity of glycine max (soybean) alpha-d-galactosidase on native human erythrocytes. the enzyme readily hydrolyzed the terminal alpha-d-galactosyl residue of the b ... | 1995 | 7579003 |
| transformation of soybean (glycine max) via agrobacterium tumefaciens and analysis of transformed plants. | | 1995 | 7581657 |
| induction of micronucleus formation in mouse splenocytes by the soy isoflavone genistein in vitro but not in vivo. | the effects of genistein (one of the major soybean isoflavones), genistein (the glucosylated form of genistein) and etoposide (a topoisomerase 11 inhibitor) have been studied in mouse splenocytes in culture. genistein (25 microm), genistein (25 microm) and etoposide (0.1 microm) all induced the production of large numbers of micronuclei; however, genistein at 12.5 or 2.5 microm had no clastogenic effect. in a second study, mice were gavaged with 20 mg genistein/kg body weight/day for 5 days (app ... | 1995 | 7590538 |
| the effect of roasting temperature applied to whole soybeans on site of digestion by steers: i. organic matter, energy, fiber, and fatty acid digestion. | a 4 x 5 youden square design was used to determine the effects of form of oil and roasting temperature of soybeans on site and extent of om, fiber, energy, lipid, and fatty acid digestion and the degree of ruminal biohydrogenation of c18 unsaturated fatty acids. four steers (373 kg +/- 30.7) with ruminal, duodenal, and ileal cannulas were fed each of five diets during five periods. diets contained (percentage of dm) corn silage (50%), alfalfa hay (24%), and a corn-urea concentrate (6%). cornstar ... | 1995 | 7592100 |
| the effects of roasting temperature applied to whole soybeans on site of digestion by steers: ii. protein and amino acid digestion. | a 4 x 5 youden square design was used to determine the effect of roasting temperature of whole soybeans on escape of cp from the rumen and disappearance of n from the small intestine in steers. four steers (average bw 373 kg +/- 30.7) cannulated at the rumen, duodenum, and ileum were fed each of five diets (1.8% of bw) over five periods. the basal diet contained corn silage (50% of diet dm), alfalfa hay (24%), corn-urea premix (6%), corn starch grits (16.6%), and soybean oil (3.4%). soybeans (16 ... | 1995 | 7592101 |
| effect of dietary raw soybeans on coccidiosis in chickens. | six battery experiments determined the effect of feeding raw soybeans (rsb) in corn-soybean diets on coccidiosis in broiler chickens. the experimental design represents a 2 x 3 factorial arrangement of treatments with three dietary levels of incorporation of rsb (0, 50, and 100%), and chickens either infected or uninfected. the coccidial species tested singularly were eimeria acervulina, eimeria maxima, eimeria necatrix, eimeria brunetti, and eimeria tenella. with all species of eimeria a signif ... | 1995 | 7603956 |
| two classes of cortical neurones labelled with vicia villosa lectin in the guinea-pig. | cell surface substance(s) containing terminal n-acetylgalactosamine (gainac) was localized in the cerebral cortex of the guinea-pig. vicia villosa and glycine max, both specific to gainac, labelled the surface of the cell body of multipolar and triangular neurones. the labelling extended to the apical dendrite and axon initial segment in the triangular neurones. they were distributed exclusively in layer 5. most of the labelled multipolar neurones contained a calcium-binding protein, parvalbumin ... | 1995 | 7605912 |
| effect of protein and starch degradation rates on rumen metabolism in taiwan native goat. | six ruminal and duodenal cannulated taiwan native goats (body weight = 20 kg) were fed 35% roughage, 65% concentrate diet (crude protein = 9.5%) in a 6 x 6 latin square design to study the effect of the combination of 3 varying starch (corn) and 2 varying protein (soybeans) ruminal degradation rates on ruminal microbial density and ruminal nutrient digestibilities. goats in treatment consisting of both rapid starch and protein ruminal degradation rates had higher total bacterial and protozoal nu ... | 1995 | 7624449 |
| the soybean gh2/4 gene that encodes a glutathione s-transferase has a promoter that is activated by a wide range of chemical agents. | transcriptional activation of the soybean (glycine max) gh2/4 gene (also referred to as gmhsp26-a) and increase in abundance of the gh2/4 mrna (also referred to as pce54) have been previously shown to occur following treatment of soybean seedlings with auxins, nonauxin analogs, heavy metals, and a variety of other agents. to determine whether the gh2/4 promoter is responsive to an array of different agents, we have analyzed the inducibility of the gh2/4 promoter fused to the beta-glucuronidase r ... | 1995 | 7630972 |
| the utilization of lupin (lupinus angustifolius) and faba bean globulins by rats is poorer than of soybean globulins or lactalbumin but the nutritional value of lupin seed meal is lower only than that of lactalbumin. | the effects of dietary sweet lupin (lupinus angustifolius, unicrop) seed meal or its insoluble fiber (nonstarch polysaccharides + lignin) on performance, digestibility and nitrogen utilization in growing rats were studied in four experiments. globulin proteins isolated from lupin, faba bean (vicia faba l. minor) or soybean (glycine max) were also incorporated into purified diets as replacements for lactalbumin (control) and the nutritional effects were evaluated. isocaloric, legume-based diets s ... | 1995 | 7643249 |
| procedures for measuring the quality of heat-treated soybeans. | soybeans were subjected to 12 heat treatments, differing in temperature and steeping (conditioning) time: control (no heat); 110 degrees c, 0 min; 110 degrees c, 30 min; 123 degrees c, 0 min; 123 degrees c, 30 min; 135 degrees c, 0 min; 135 degrees c, 30 min; 146 degrees c, 0 min; 146 degrees c, 15 min; 146 degrees c, 30 min; 153 degrees c, 30 min; and 160 degrees c, 30 min. these soybean treatments were tested for protein dispersibility index, absorbance at 420 nm, adin, pepsin-insoluble n, and ... | 1995 | 7673525 |
| deletion analysis and localization of sbprp1, a soybean cell wall protein gene, in roots of transgenic tobacco and cowpea. | sbprp1 is a member of the soybean (glycine max l. merr) proline-rich cell wall protein family and is expressed at high levels in root tissue. to characterize the sequences required for this expression, we have fused 1.1 kb of upstream flanking dna sequence from an sbprp1 genomic clone to a gene encoding beta-glucuronidase (gus). this construct was introduced into tobacco using agrobacterium tumefaciens-mediated transformation. histochemical staining of gus activity in transgenic tobacco indicate ... | 1993 | 7678758 |
| comparison of the mast chemiluminescent assay system with rast and skin tests in allergic children. | the mast chemiluminescent assay (cla) system uses enzyme-linked antihuman ige and a chemiluminogenic substance to determine specific ige antibodies. the mast cla system therefore not only makes unnecessary the precautions required for handling isotopic materials but has the advantage of long-term reagent stability. the mast cla system assay protocol consists of three steps: overnight incubation of serum, a 4-hour incubation with enzyme-labeled antibody, and a 30-minute chemiluminescent reaction, ... | 1993 | 7679259 |
| postnatal development and localization of an n-acetylgalactosamine containing glycoconjugate associated with nonpyramidal neurons in cat visual cortex. | we have analyzed the appearance of n-acetylgalactosamine containing glycoconjugates by staining sections of fixed cat visual cortex with the lectins from vicia villosa (vva) and glycine max (sba) conjugated to fluorescent labels, horseradish peroxidase, or biotin. the appearance of lectin staining during postnatal development followed an inside-out gradient starting in the deep cortical layers at the fourth postnatal week, successively including more of the superficial layers during the first th ... | 1993 | 7681454 |
| characterization of glycoconjugate expression during development of meckel's cartilage in the rat. | the staining patterns of 24 biotinylated lectins were analyzed in serial sections of the mandible of 13- to 21-day-old rat embryos by means of the avidin-biotin-peroxidase method. a ubiquitous distribution of binding sites was demonstrated after incubation with con a (canavalia ensiformis), dsl (datura stramonium; except bone matrix), and wga (triticum vulgare). ecl (erythrina cristagalli), gsl i (griffonia simplicifolia), sja (saphora japonica), vvl (vicia villosa), dba (dolichus biflorus), uea ... | 1995 | 7717533 |
| native and asialo-tamm-horsfall glycoproteins as important ligands for the detection of galnac beta 1-->and gal beta 1-->4glcnac active lectins. | the binding properties of human tamm-horsfall sd(a+) urinary glycoprotein(thgp) and asialo-thgp with various applied lectins was investigated by quantitative precipitin and precipitin inhibition assays. both glycoproteins completely precipitated abrus precatorius agglutinin(apa). they also reacted well with wistaria floribunda (wfa), glycine max (soybean, sba), and ricinus communis agglutinins and precipitated over 78% of the lectin nitrogen added, but reacted poorly or weakly with all alpha-ano ... | 1995 | 7726822 |
| changes in glycan distribution within the porcine interhaemal barrier during gestation. | changing patterns of glycan distribution are described in porcine placentae at 15, 19, 26, 43, 58, 69 and 109 days gestation, using a carefully selected panel of lectins that allowed partial analysis of saccharide classes and sequences. the lectins used were from galanthus nivalis, pisum sativum, phaseolus vulgaris (leukohaemagglutinin), triticum vulgaris, tetragonolobus purpureus, ulex europaeus-1, arachis hypogaea, erythrina cristagalli, glycine max, maclura pomifera, wisteria floribunda, doli ... | 1995 | 7736552 |
| cloning, nucleotide sequence and expression of the bifunctional dihydrofolate reductase-thymidylate synthase from glycine max. | cdnas encoding the bifunctional dihydrofolate reductase-thymidylate synthase from glycine max were isolated and sequenced. the 1794 base full length cdna contains a single open reading frame of 1593 bases. the predicted size of the encoded protein is 530 amino acids with a molecular weight of 59,707. the protein has two domains: a 226 residue dhfr domain in the n-terminus, which is over 30% identical to human dhfr or the dhfr domain of protozoal dhfr-ts, and a 304 residue thymidylate synthase (t ... | 1995 | 7742362 |
| simultaneous induction of postabscission and germination mrnas in cultured dicotyledonous embryos. | cloned mrnas identify three programs of gene expression in cotton (gossypium hirsutum l.) embryos that are associated with the maturation (reserve accumulation) stage, the postabscission stage, which is marked by expression of late-embryogenesis-abundant (lea) mrnas, and germination (broadly defined as including all events through early postgerminative growth). in order to test if the regulation of these programs is the same in other dicotyledonous species, their expression was studied in normal ... | 1994 | 7764404 |
| purification and molecular characterization of a soybean polygalacturonase-inhibiting protein. | a polygalacturonase-inhibiting protein (pgip) was detected in soybean (glycine max (l.) merr.) seedlings. the protein was purified from germinating seeds and appeared to consist of at least three components with very close molecular weights (between 37 and 40 kda) but each showing a unique n-terminal sequence. primers specific for n-terminal and c-terminal nucleotide sequences of field bean (phaseolus vulgaris l.) pgip were used in a polymerase chain reaction (pcr) on soybean dna, and only one a ... | 1994 | 7765794 |
| isolation and characterization of gmsti, a stress-inducible gene from soybean (glycine max) coding for a protein belonging to the tpr (tetratricopeptide repeats) family. | in close vicinity of two fus nuclear genes (chloroplast-specific translation elongation factor cef-g) of soybean (glycine max) we localized a split nuclear gene coding for a protein with tetratricopeptide repeats (tpr). a full-length cdna was sequenced (1871 nucleotides). it encodes a protein (569 amino acids) with high sequence identity to the yeast sti1 stress-inducible and the human transformation-sensitive ief ssp 3521 protein which both carry tpr elements. the soybean gene is heat-inducible ... | 1995 | 7766904 |
| lectin histochemistry of normal and herpesvirus-infected bovine nasal mucosa. | proliferation of pasteurella haemolytica serotype 1 in the nasal cavity following stress or viral infection is an important event in the pathogenesis of bovine pneumonic pasteurellosis. enhanced adhesion of p. haemolytica to nasal mucosa could be one factor that predisposes animals to this proliferation. nasal mucosa from normal and bovine herpesvirus-1 (bhv1)-infected cattle were examined histochemically for their glycoconjugate composition. twenty lectins were screened, six of which were chose ... | 1995 | 7771054 |
| estrous cycle-related alterations in the expression of glycoconjugates and lectins in the mouse endometrium shown histochemically. | alterations of the hormonal status may influence diverse cell features relevant to intra- and intercellular communication. we studied histochemically the expression of glycans and endogenous sugar-binding proteins (endolectins) in the mouse endometrium during the estrous cycle. the avidin-biotin-peroxidase complex (abc) technique was used on paraffin sections with a panel of biotinylated lectins and neoglycoproteins. stage-specific changes were observed with galanthus nivalis agglutinin (gna), u ... | 1995 | 7778095 |
| narbonin, a novel 2s protein from vicia narbonensis l. seeds: cdna, gene structure and developmentally regulated formation. | cdna and genomic clones encoding narbonin, a 2s globulin from the seed of narbon bean (vicia narbonensis l.), were obtained using the polymerase chain reaction (pcr) and sequenced. the full-length cdna as well as genomic clones contain a single open reading frame (orf) of 873 bp that encodes a protein with 291 amino acids comprising the mature narbonin polypeptide (m(r) ca. 33 100) and an initiation methionine. the deduced amino acid sequence lacks a transient n-terminal signal peptide. the geno ... | 1995 | 7787188 |
| galactose-containing glycoconjugates of the iris, the aqueous outflow passages and the cornea in capsular glaucoma. a lectin histochemical study. | the aim of the study was to compare galactose-containing glycoconjugates of the iris, the aqueous outflow passages and the cornea with exfoliation material in capsular glaucoma. | 1995 | 7797082 |
| the normal mycoflora of commodities from thailand. 2. beans, rice, small grains and other commodities. | part of a comprehensive study of fungi occurring in commodities in thailand, this paper reports results from 276 samples of mung beans, rice, sorghum and soybeans as well as other minor crops. samples for major commodities were taken from farmers' stocks, middlemen and retail outlets, while those for minor crops were mostly from retail. each sample was divided into two portions, one being examined in bangkok and the second at north ryde. mycological examinations were carried out by direct platin ... | 1994 | 7811571 |
| release of free fatty acids from raw or processed soybeans and subsequent effects on fiber digestibilities. | two in vitro experiments were conducted to determine the rates of lipolysis and the extent of biohydrogenation of fat from raw or processed soybeans and to examine the subsequent effects on fiber digestibilities. in experiment 1, substrates containing soy oil, raw soybeans, extruded soybeans, and soybeans roasted at 132, 146, or 163 degrees c were incubated with ruminal contents for 2, 4, 6, 12, or 24 h; and release of ffa was measured. the ffa released from substrates containing soy oil, extrud ... | 1994 | 7814716 |
| evaluation of a new high protein variety of soybeans as a source of protein and energy for dairy cows. | twenty holstein cows in midlactation were used in a latin square design to evaluate the nutritional quality of a high protein soybean (cp 45%) fed raw or roasted. treatments were 1) control (soybean meal); 2) conventional soybean (maple isle), raw; 3) conventional soybean, roasted; 4) high protein soybean (ac proteus), raw; and 5) high protein soybean, roasted. diets were formulated to be isonitrogenous and consisted of approximately 10% whole crop barley silage, 15% corn silage, 19% alfalfa sil ... | 1994 | 7814730 |
| lectin-histochemical analysis of glycans in ovine and bovine near-term placental binucleate cells. | chorionic binucleate cells (bnc) occur in several ruminants including cow, deer, goat and sheep. they migrate through the chorionic tight junction to fuse with uterine epithelial cells and discharge their granules into maternal connective tissue. we have compared the bnc of near-term, resin-embedded, ovine and bovine placentae using 15 biotinylated lectins and an avidinperoxidase revealing system. there was pronounced conservation of saccharides between the two species. several sub-types of n-gl ... | 1994 | 7850870 |
| consumption of diets containing raw soya beans (glycine max), kidney beans (phaseolus vulgaris), cowpeas (vigna unguiculata) or lupin seeds (lupinus angustifolius) by rats for up to 700 days: effects on body composition and organ weights. | feeding trials have been done with rats to assess the effects of long-term (700 d) consumption of diets based on raw cowpeas (vigna unguiculata; moderate bowman-birk inhibitor content, low lectin content), lupin seeds (lupinus angustifolius; low lectin and protease inhibitor content) or soya beans (glycine max; high kunitz inhibitor content, moderate bowman-birk inhibitor content, moderate lectin content) or diets containing low levels of raw kidney bean (phaseolus vulgaris; high lectin content, ... | 1995 | 7857911 |
| role of chemopreventers in human diet. | recent research has confirmed that many common foods contain nonnutritive components that may provide protection against chronic disease including some forms of cancer. these naturally occurring compounds, which possess anticarcinogenic and other beneficial properties, are referred to as chemopreventers. the predominant mechanism of their protective action is due to their antioxidant activity and the capacity to scavenge free radicals. among the most investigated chemopreventers are some vitamin ... | 1994 | 7867212 |
| duodenase, a new serine protease of unusual specificity from bovine duodenal mucosa. purification and properties. | in this paper, data are presented on purification and properties of a new serine endopeptidase (duodenase) isolated from bovine duodenum mucosa. the enzyme has been purified to homogeneity by combinations of ammonium sulphate fractionation, carboxymethyl-cellulose 52 chromatography, and affinity chromatography on sepharose 4b with kunitz soybean trypsin inhibitor as a ligand. some physicochemical properties of this protease have been investigated. the molecular mass of the purified duodenase was ... | 1995 | 7867648 |
| [influence of foods on the posterior longitudinal ligament of the cervical spine and serum sex hormones]. | to investigate the involvement of sex hormones as a potential cause of ossification in the posterior longitudinal ligament (opll) of the cervical spine, we experimentally examined the influence of ingested foods on the serum levels of sex hormones and changes in the spinal ligament. castrated rabbits were administered a sex hormone and raised with special feed. assay of the serum sex hormone levels, analysis of the carbon and nitrogen isotope ratios in body hairs, and histological studies were c ... | 1994 | 7876685 |
| the effects of phytoestrogens on neonatal rat uterine growth and development. | phytoestrogens found in clover, alfalfa, and soybeans have caused reproductive toxicity in several mammalian species. other estrogens, such as diethylstilbestrol (des), are developmental toxicants, reducing uterine estrogen receptor (er) concentration, altering uterine growth, and eliciting reproductive tract abnormalities in the rat. the present study examines the effects of the phytoestrogens coumestrol and equol on the developing rat uterus. various doses of these compounds were injected sc o ... | 1995 | 7878071 |
| [the human spleen in idiopathic thrombocytopenic purpura]. | idiopathic (autoimmune) thrombocytopenic purpura (itp, aitp) represents a relatively frequent impairment. it involves a syndrome of various diseases with a shortened thrombocytes survival caused by anti-platelet antibodies. the majority of cases are of secondary character. spleenectomy often evokes a complete remission of thrombocytopenia. the study describes morphologic findings in spleens of 30 patients with the clinical diagnosis of itp/aitp. the findings were gained by light microscopy from ... | 1994 | 7882065 |
| effect of dietary canola on fatty acid composition of bovine adipose tissue, muscle, kidney, and liver. | the purpose of this study was to determine effects of feeding canola and soybean products as protein supplements on fatty acid composition of adipose tissue and muscle of slaughter bulls and steers and on fatty acid composition of kidney and liver total lipids of bulls. products included canola meal (cm), extruded canola (ec, full-fat), ground canola (gc, full-fat), soybean meal (sbm), and extruded soybeans (es, full-fat). tissues were obtained at slaughter from 75 crossbred beef bulls and 50 cr ... | 1994 | 7883635 |
| overview of proposed mechanisms for the hypocholesterolemic effect of soy. | a large body of literature indicates that protein from soybeans reduces blood cholesterol concentrations in experimental animals as well as in humans. the mechanism and component of soy responsible has not been established fully. some suggest that when soy protein is fed, cholesterol absorption and/or bile acid reabsorption is impaired. this is observed in some animal species, such as rabbits and rats, but not in humans nor when amino acids replace intact soy protein. others propose that changes ... | 1995 | 7884541 |
| saponins as anticarcinogens. | saponins, which are present in plants, have been suggested as possible anticarcinogens. they possess surface-active characteristics that are due to the amphiphilic nature of their chemical structure. the proposed mechanisms of anticarcinogenic properties of saponins include direct cytotoxicity, immune-modulatory effects, bile acid binding and normalization of carcinogen-induced cell proliferation. however, the anticarcinogenic effects of saponins from commonly consumed plant foods have not been ... | 1995 | 7884557 |
| the evidence for soybean products as cancer preventive agents. | there is much evidence suggesting that compounds present in soybeans can prevent cancer in many different organ systems. the evidence for specific soybean-derived compounds having a suppressive effect on carcinogenesis in animal model systems is limited, however. there is evidence that the following isolated soybean derived products suppress carcinogenesis in vivo: a protease inhibitor, the bowman-birk inhibitor, inositol hexaphosphate (phytic acid) and the sterol beta-sitosterol. other compound ... | 1995 | 7884559 |
| possible adverse effects of soybean anticarcinogens. | for soybeans to serve as a good source of protein for feeding animals as well as humans, a certain amount of heat treatment or some other form of processing must be applied. this is because there are present in soybeans certain heat-labile factors that exert an adverse effect on the nutritional value of the protein. the so-called protease inhibitors have received the most attention in this regard and have been shown to exert their antinutritional effect in the short term by causing pancreatic hy ... | 1995 | 7884560 |
| antioxidant and antipromotional effects of the soybean isoflavone genistein. | antioxidant and antipromotional effects of the soybean isoflavone genistein have been studied in hl-60 cells and the mouse skin tumorigenesis model. effects of structure-related flavone/isoflavones on hydrogen peroxide (h2o2) production by 12-o-tetradecanoylphorbol-13-acetate (tpa)-activated hl-60 cells and superoxide anion (o2-) generation by xanthine/xanthine oxidase were compared. of tested isoflavones, genistein is the most potent inhibitor among tpa-induced h2o2 formation by (dimethyl sulfo ... | 1995 | 7892286 |
| control of de novo purine biosynthesis genes in ureide-producing legumes: induction of glutamine phosphoribosylpyrophosphate amidotransferase gene and characterization of its cdna from soybean and vigna. | soybean (glycine max) and mothbean (vigna aconitifolia) cdna clones encoding glutamine phosphoribosylpyrophosphate amidotransferase (prat), the first enzyme of the de novo purine biosynthesis pathway, have been isolated from nodule cdna libraries. the amino acid sequence deduced from soybean clone showed > 85% homology to the prat sequence of mothbean and 33-47% homology to those of bacteria, yeast, chicken, rat and human. the soybean clone encodes a protein with an n-terminal sequence resemblin ... | 1995 | 7894513 |
| high-performance liquid chromatographic assay of isoflavonoids and coumestrol from human urine. | a rapid, sensitive and precise diode-array reversed-phase hplc method was developed for human urine analysis of the most common dietary isoflavones daidzein, genistein, formononetin and biochanin-a, their mammalian metabolites equol and o-desmethylangolensin, and of coumestrol, another commonly occurring phytoestrogen. analytes were isolated and concentrated by solid-phase extraction and separated by hplc followed by identification through retention times and uv scans, and in the case of coumest ... | 1994 | 7894693 |
| identification of a homeobox-containing gene with enhanced expression during soybean (glycine max l.) somatic embryo development. | homeotic gene are key 'switches' that control developmental processes. homeotic genes containing the consensus 'homeobox' domain have been identified from a number of organisms including drosophila melanogaster, caenorhabditis elegans, homo sapiens, and zea mays. although homeotic genes have been demonstrated to be important in embryo development of some insects, amphibians, and mammals, there are no reports of their involvement in plant embryogenesis. here, we report the isolation and character ... | 1994 | 7907232 |
| structures of nodulation factors from the nitrogen-fixing soybean symbiont rhizobium fredii usda257. | we have isolated and characterized the extracellular nod factors of rhizobium fredii usda257, a nitrogen-fixing symbiont of soybean [glycine max (l.) merr.] and several other legume species. these signals are produced upon exposure to the isoflavone genistein and consist of a series of substituted, beta 1,4-linked tri-, tetra-, and pentamers of n-acetylglucosamine. n-vaccenic acid replaces acetate on the nonreducing residue, and the reducing residue contains alpha-linked 2-o-methylfucose on carb ... | 1994 | 7918395 |
| novel isoprenylated proteins identified by an expression library screen. | isoprenylated proteins are involved in eukaryotic cell growth and signal transduction. the protein determinant for prenylation is a short carboxyl-terminal motif containing a cysteine, to which the isoprenoid is covalently attached via thioether linkage. to date, isoprenylated proteins have almost all been identified by demonstrating the attachment of an isoprenoid to previously known proteins. thus, many isoprenylated proteins probably remain undiscovered. to identify novel isoprenylated protei ... | 1994 | 7929216 |
| a method of purifying feline t lymphocytes from peripheral blood using the plant lectin from pisum sativum. | the binding properties of several plant lectins on feline peripheral blood lymphocytes (pbl) were examined by flow cytometry to reveal if any could serve as probes for identifying specific lymphocyte subclasses. most of the lectins bound > or = 90% of pbl in a uniform manner. the exceptions were the lectins from glycine max, phaseolus limensis, and dolichos biflorus, which bound only a proportion of the pbl. the differential binding of the lectins to lymphocyte subclasses was examined by compari ... | 1994 | 7930648 |
| histochemical study by lectin binding of surface glycoconjugates in the developing olfactory system of rat. | lectin-binding histochemistry was used to investigate the distribution and density of defined carbohydrate sequences on the cell surface glycoproteins of the olfactory receptors of rat during development. the olfactory and vomeronasal receptors showed a positive labelling after biotinylated lycopersicum esculentum lectin binding on embryonic day 16 (e16), while horseradish peroxidase-labelled glycine max, bandeiraea simplicifolia (bsa-i) and its b4 isomer bsa-i-b4 agglutinins started to label fr ... | 1994 | 7942093 |
| in situ characterization of the human hyalocyte. | to provide more definitive evidence regarding the cellular origin of hyalocytes and to establish a basis for their identification in tissue specimens. | 1994 | 7945040 |
| the distribution of n-acetylgalactosamine in the cochlear nucleus of the gerbil revealed by lectin binding with soybean agglutinin. | a horseradish peroxidase conjugated lectin from glycine max (soy bean agglutinin; sba) was used to characterise the distribution of n-acetylgalactosamine in the cochlear nucleus of the mongolian gerbil. sba bound differentially to a variety of structures within the cochlear nucleus. specific sba labelling was associated with large non-granule neurones of variable size and shape throughout the cochlear nucleus. compared to adjacent nissl-stained sections 80% of the non-granule cells in the dorsal ... | 1994 | 7961177 |
| high-performance capillary electrophoresis for characterization of hapten-protein conjugates used for production of antibodies against soyasaponin i. | micellar electrokinetic capillary chromatography using sodium cholate as the micellar phase has been investigated for characterization of hapten-protein conjugates. special focus has been placed on the hapten soyasaponin i which is a quantitatively dominating glycoside in seeds of several legumes including pea (pisum sativum l.) and soybean [glycine max (l.) merr.]. soyasaponin i has been isolated from pea and used as hapten for production of anti-saponin specific polyclonal antibodies. soyasapo ... | 1994 | 7981825 |
| recovery of amino acids at the distal ileum for determining apparent and true ileal amino acid digestibilities in growing pigs fed various heat-processed full-fat soybean products. | ten crossbred gilts fitted with simple t-cannulas at the distal ileum were used to determine the apparent and true ileal amino acid digestibilities in five different soybean products: extruded, jetsploded, micronized, or roasted full-fat soybeans (ffsb) and soybean meal (sbm). the gilts with an average initial body weight of 36 kg were fed the different diets according to a replicated 5 x 5 latin square design. gilts were fed twice daily at 0800 and 1830 at 2.6 times maintenance energy requireme ... | 1994 | 7982832 |
| nod factors of rhizobium are a key to the legume door. | symbiotic interactions between rhizobia and legumes are largely controlled by reciprocal signal exchange. legume roots excrete flavonoids which induce rhizobial nodulation genes to synthesize and excrete lipo-oligosaccharide nod factors. in turn, nod factors provoke deformation of the root hairs and nodule primordium formation. normally, rhizobia enter roots through infection threads in markedly curled root hairs. if nod factors are responsible for symbiosis-specific root hair deformation, they ... | 1994 | 7984092 |
| seed-specific gene activation mediated by the cre/lox site-specific recombination system. | the cre/lox site-specific recombination system was used to activate a transgene in a tissue-specific manner. cre-mediated activation of a beta-glucuronidase marker gene, by removal of a lox-bounded blocking fragment, allowed the visualization of the activation process. by using seed-specific promoters, the timing and efficiency of gene activation could be followed within the developing tobacco (nicotiana tabacum) embryo. to serve as a basis for analyzing gene expression after-cre-mediated activa ... | 1994 | 7991679 |
| experimental evidence of a plant meridian system: ii. the effects of needle acupuncture on the temperature changes of soybean (glycine max). | when stainless steel needles were inserted opposite each other in the stem near the unifoliolate buds of soybean plants and retained there for the whole experiment, leaf temperatures increased for two consecutive days. following acupuncture, the mean temperature of the main vein in the first and second days increased 0.59 degree c and 0.48 degree c, respectively, while the temperature of mesophyll increased only 0.50 degree c and 0.47 degree c in the first and second days. these results are very ... | 1994 | 7992809 |
| position 713 is critical for catalysis but not iron binding in soybean lipoxygenase 3. | the role of asparagine-713 in iron atom incorporation and catalysis in soybean lipoxygenase 3 was investigated using site-directed mutagenesis. a full-length cdna for the lipoxygenase isoenzyme was obtained from a library derived from soybeans cv. provar. protein with native specific activity at ph 7.4 was obtained from expression in escherichia coli. two recent structure reports provided conflicting views about the participation of the side chain of asparagine-694 in the coordination of the iro ... | 1994 | 7999759 |
| cloning and expression of the soybean dapa gene encoding dihydrodipicolinate synthase. | the rate-limiting step in the pathway for lysine synthesis in plants is catalyzed by the enzyme dihydrodipicolinate synthase (ds). we have cloned the portion of the soybean (glycine max cv. century) dapa cdna that encodes the mature ds protein. expression of the cloned soybean cdna, as a lacz fusion protein was selected in a dapa- escherichia coli auxotroph. the ds activity of the fusion protein was characterized in e. coli extracts. the ds activity of the fusion protein was inhibited by lysine ... | 1994 | 8000011 |
| identification and characterization of the nolyz genes of bradyrhizobium japonicum. | characterization of an isoflavone-inducible locus closely linked to the common nod genes of bradyrhizobium japonicum usda110 led to the discovery of two open reading frames, designated noly and nolz. these open reading frames are preceded by a sequence with strong similarity to a consensus nodd-binding site (nod box). studies utilizing a nolz'-'lacz fusion indicated that inducible expression is dependent upon both nodd1 and nodw, transcriptional regulators that are required for the expression of ... | 1994 | 8012039 |
| plant delta-aminolevulinic acid dehydratase. expression in soybean root nodules and evidence for a bacterial lineage of the alad gene. | we isolated a soybean (glycine max) cdna encoding the heme and chlorophyll synthesis enzyme delta-aminolevulinic acid (ala) dehydratase by functional complementation of an escherichia coli hemb mutant, and we designated the gene alad. ala dehydratase was strongly expressed in nodules but not in uninfected roots, although alad mrna was only 2- to 3-fold greater in the symbiotic tissue. light was not essential for expression of alad in leaves of dark-grown etiolated plantlets as discerned by mrna, ... | 1994 | 8016269 |
| vitamin b12 production by citrobacter freundii or klebsiella pneumoniae during tempeh fermentation and proof of enterotoxin absence by pcr. | the influence of some fermentation parameters on vitamin b12 formation by strains of citrobacter freundii and klebsiella pneumoniae isolated from indonesian tempeh samples during tempeh fermentation was investigated. a decrease in fermentation temperature from 32 to 24 degrees c led to a decrease in vitamin b12 formation. inoculation of soybeans with different numbers of cells of c. freundii at the beginning of solid-substrate fermentation showed that only the velocity of vitamin formation and n ... | 1994 | 8017933 |
| characterization of glycoconjugates in the endometrium of the miniature pig during different reproductive periods. | lectin-binding glycoconjugates in the endometrium of miniature pigs were determined for sexually immature, dioestrus, oestrus and early pregnancy periods, using five horseradish peroxidase-labelled lectins. apparent changes associated with sexual maturity were increases in binding intensity of wheat germ agglutinin (wga), glycine max agglutinin (sba) and ulex europaeus agglutinin (uea) and the appearance of ricinus communis agglutinin (rca) binding. the binding intensity of wga, sba and uea furt ... | 1994 | 8021858 |
| purification and physicochemical characterization of soyatoxin, a novel toxic protein isolated from soybeans (glycine max). | physicochemical characterization and biological properties of a new toxic protein isolated from soybeans (glycine max) is reported. the purification procedure consisted basically of ammonium sulfate fractionation, ion exchange, and affinity chromatographies, the latter being used for the removal of the seed's lectin and of its trypsin inhibitor. the highly purified protein, designated soyatoxin, is a single chain acidic protein (pi 4.4-4.6) of 21 kda, dependent on reduced thiol groups to maintai ... | 1994 | 8037448 |
| lactational performance of dairy cows fed raw soybeans, with or without animal by-product proteins, or roasted soybeans. | twelve multiparous holstein cows averaged 10 wk postpartum and were used in a replicated 3 x 3 latin square design to compare two feeding strategies for increasing the ratio of dietary undegradable to degradable protein. treatments were raw soybeans, with or without meat and bone meal plus blood meal, and roasted soybeans as the primary protein supplements. meat and bone meal and blood meal were fed at 4.0 and .9% of dietary dm, respectively. basal diets were 30% alfalfa silage, 18% corn silage, ... | 1994 | 8046075 |
| in vitro determination of ruminal protein degradability using [15n]ammonia to correct for microbial nitrogen uptake. | an in vitro procedure was developed to estimate rate and extent of ruminal protein degradation using [15n]h3 to quantify uptake of protein degradation products for microbial protein synthesis. incubations were conducted for 6 h in stirrer flasks with ruminal inoculum plus buffer, reducing solution, pectin, soluble carbohydrates, and added (15nh4)2so4. seven protein concentrates were tested in the system. samples of media were analyzed for 15n enrichment of nh3, microbial n, and total solids n. d ... | 1994 | 8056683 |
| formation of b-vitamins by bacteria during the soaking process of soybeans for tempe fermentation. | the formation of b-vitamins (nicotinic acid and nicotinamide, thiamine, vitamin b6 and vitamin b12) during the soaking of soybeans by bacteria, isolated from tempe, was investigated. among the isolates examined no vitamin b6 producer was found. after inoculation of the soaking soybeans with citrobacter freundii, klebsiella pneumoniae, pseudomonas fluorescens and streptococcus spp. the concentrations of vitamin b12 increased significantly. for the first time it was found that soakings inoculated ... | 1994 | 8060790 |
| cloning of a higher-plant plastid omega-6 fatty acid desaturase cdna and its expression in a cyanobacterium. | oligomers based on amino acids conserved between known plant omega-3 and cyanobacterium omega-6 fatty acid desaturases were used to screen an arabidopsis cdna library for related sequences. an identified clone encoding a novel desaturase-like polypeptide was used to isolate its homologs from glycine max and brassica napus. the plant deduced amino acid sequences showed less than 27% similarity to known plant omega-6 and omega-3 desaturases but more than 48% similarity to cyanobacterial omega-6 de ... | 1994 | 8066133 |
| characterization of a soybean beta-conglycinin-degrading protease cleavage site. | protease c1, an enzyme from soybean (glycine max [l.] merrill cv amsoy 71) seedling cotyledons, was previously determined to be the enzyme responsible for the initial degradation of the alpha' and alpha subunits, but not the beta subunit, of beta-conglycinin storage protein. the sizes of the proteolytic products generated by the action of protease c1 suggest that the cleavage sites on the alpha' and alpha subunits of beta-conglycinin may be located in their n-terminal domain, which is not found ... | 1994 | 8115542 |
| raw soybeans and roasted soybeans of different particle sizes. 2. fatty acid utilization by lactating cows. | this study was conducted to examine the effects of feeding whole raw soybeans and roasted soybeans of different particle sizes on utilization by ruminal bacteria and lactating cows. five cows with ruminal and duodenal cannulas were used in a 5 x 5 latin square design. diets contained 55% corn silage and 45% concentrate. treatments were control (ca soaps at 4.24% of dietary dm), whole raw soybeans, whole roasted soybeans, cracked roasted soybeans, or ground roasted soybeans. all soybean diets con ... | 1994 | 8120185 |
| role of intramolecular high-mannose chains in the folding and assembly of soybean (glycine max) lectin polypeptides: studies by the combined use of spectroscopy and gel-filtration size analysis. | it was previously reported [nagai, k. & yamaguchi, h. (1993) j. biochem. 113, 123-125] that intramolecular high-mannose chains are essential for reconstitution of soybean lectin from denatured subunits. to obtain more detailed information on the role of the intramolecular high-mannose chains in the folding and assembly of soybean lectin polypeptides, the effects of asparagine-linked oligosaccharides, man9glcnac2asn (m9-asn) and glc1-3man9glcnac2asn (gm9-asn), on the reconstitution of soybean lec ... | 1993 | 8138539 |
| implications of antinutritional components in soybean foods. | there are a number of components present in soybeans that exert a negative impact on the nutritional quality of the protein. among those factors that are destroyed by heat treatment are the protease inhibitors and lectins. protease inhibitors exert their antinutritional effect by causing pancreatic hypertrophy/hyperplasia, which ultimately results in an inhibition of growth. the lectin, by virtue of its ability to bind to glycoprotein receptors on the epithelial cells lining the intestinal mucos ... | 1994 | 8142044 |
| ulex europaeus i and glycine max bind to the human olfactory bulb. | the distribution of binding sites for the fucose-selective lectin ulex europaeus i and the terminal n-acetylgalactosamine-selective lectin glycine max in the human olfactory bulb were studied. these lectins bound to primary olfactory axons in the olfactory nerve layer and the glomerular layer. they also bound to fibers located in the deeper layers such as the external plexiform layer and the granular layer. furthermore they projected to the olfactory stalk but not in the cerebrum. the deeper pro ... | 1993 | 8152605 |
| hypocotyl expression and light downregulation of the soybean tubulin gene, tubb1. | the tubb1 beta-tubulin gene of glycine max (previously named s beta 1) is highly expressed only in rapidly elongating regions of etiolated seedling hypocotyls and this expression is strongly downregulated when the seedlings are exposed to light. primer extension demonstrated that the gene was transcribed in these tissues and contained two sites of transcriptional initiation. to determine the mechanism regulating tubb1 expression, a chimeric reporter gene was constructed by fusing 5' upstream reg ... | 1994 | 8180620 |
| variable stability of antibiotic-resistance markers in bacillus cereus uw85 in the soybean rhizosphere in the field. | we compared the stability of antibiotic-resistance markers in strains derived from bacillus cereus uw85 in culture media and in the soybean rhizosphere in a growth chamber and in the field. we studied two independent, spontaneous mutants resistant to neomycin, three independent, spontaneous mutants resistant to streptomycin, and strains carrying plasmid pbc16, which encodes tetracycline resistance. antibiotic-resistance markers were maintained in populations of all uw85 derivatives in culture an ... | 1993 | 8180735 |
| infection of soybean and pea nodules by rhizobium spp. purine auxotrophs in the presence of 5-aminoimidazole-4-carboxamide riboside. | purine auxotrophs of various rhizobium species are symbiotically defective, usually unable to initiate or complete the infection process. earlier studies demonstrated that, in the rhizobium etli-bean symbiosis, infection by purine auxotrophs is partially restored by supplementation of the plant medium with 5-amino-imidazole-4-carboxamide (aica) riboside, the unphosphorylated form of the purine biosynthetic intermediate aicar. the addition of purine to the root environment does not have this effe ... | 1994 | 8195084 |
| lectins as markers of rumen epithelial cell differentiation. | lectins of different carbohydrate specificities (gna (galanthus nivalis), con a (canavalia ensiformis), vfl (vicia faba), psl (pisum sativum), lca (lens culinaris), pna (arachis hypogaea; with or without prior neuraminidase treatment), wga (triticum vulgare), sba (glycine max), uea-i (ulex europaeus), lpa (limulus polyphemus), bs-i b4 (bandeiraea simplicifolia, isolectin b4)) were explored for use as differentiation markers of rumen epithelial cells in vivo and in vitro. lectins specific for man ... | 1994 | 8206789 |
| phytogrowth-inhibitory activities of sulfur-containing compounds. ii. the inhibitory activities of thiosalicylic acid and dihydro-2(3h)-thiophenone-related compounds on plant growth. | thiosalicylic acid (i) showed rather strong inhibitory activity on the growth of roots of all plants treated except abelmoschus esculentus moench at the concentration of 5.0 x 10(-4) m. this compound strongly inhibited the growth of the root of echinochloa utilis ohwi et yabuno even at the low concentration of 5.0 x 10(-5) m. dihydro-2(3h)-thiophenone (vii) also exhibited inhibitory activity on the growth of roots of all plants treated except glycine max merrill. both compounds inhibited the ger ... | 1993 | 8220332 |
| [reconsideration of the role of aflatoxins in public health]. | the pathogenicity of aflatoxins for human has been largely demonstrated. although aflatoxin contamination of some foodstuffs has been unquestionably established, stress should be laid upon a certain lack of information: some of the affected foodstuffs have been "forgotten" in the enumeration of contaminated products (especially soybeans); relations between mycotoxins and food balance have not yet been taken into account: for instance, the influence of some vitamins, of various compounds (caroten ... | 1993 | 8221179 |
| rumen fermentation and lactation performance of cows fed roasted soybeans and tallow. | sixteen multiparous holstein cows were in a replicated 4 x 4 latin square design with 21-d periods to examine the effects of incremental tallow addition to diets containing whole roasted soybeans on rumen fermentation and lactational performance. total mixed rations were fed for ad libitum intake and contained, on a dm basis, 33% alfalfa silage, 12% corn silage, 14% roasted soybeans, and 41% concentrate based on ground corn and soybean meal. treatments were 0, 1, 2, or 3% supplemental tallow. di ... | 1993 | 8227669 |
| lupins (lupinus albus) as a protein supplement for lactating holstein dairy cows. | three experiments were conducted to determine optimal substitution of lupin for soybean meal in dairy cattle diets; the effects of whole versus ground lupins and the effects of whole lupins versus whole soybeans on milk production were examined. in the first experiment, 57 cows were assigned randomly to treatments in blocks of five by calving order within parity. treatments began 22 d postpartum and continued through d 140. diets were isonitrogenously balanced using soybean meal as control: lupi ... | 1993 | 8227670 |
| occurrence of antimicrobial activities of bacteria from soybean leaf spots. | bacteria were isolated from leaf spots of field grown soybeans during two growing seasons. the leaf spots yielded up to 4 different species and a total population size of about 10(7)-10(8) bacteria/cm2. the majority of the 192 isolates belonged to the species pseudomonas syringae pv. glycinea (55%), causing leaf spots of bacterial blight on soybeans, and erwinia herbicola (22%). the remaining isolates included bacteria from other genera, but occurred occasionally. the determination of biological ... | 1993 | 8229675 |
| cancer prevention by protease inhibitors. | there is now compelling evidence that certain protease inhibitors have strong anticarcinogenic activity. a particular protease inhibitor derived from soybeans, the bowman-birk inhibitor, appears to be highly promising as a cancer chemopreventive agent. in this article, studies performed to characterize an extract of soybeans containing the bowman-birk inhibitor, termed bowman-birk inhibitor concentrate, are described. | 1993 | 8234219 |
| miso from peas (pisum sativum) and beans (phaseolus vulgaris) of domestic origin. fermented foods from agricultural products in europe. ii. | miso is a fermented soybean paste widely used in japan as a soup base or as a seasoning agent. koji (cereal grains with the mold aspergillus oryzae) serves as enzyme source. peas (pisum sativum) and beans (phaseolus vulgaris) of german origin can be used as substitutes for soybeans in the preparation of miso-like products. the legumes (peas, beans and soybeans for comparison) are washed, soaked in boiled water, dehulled and cooked for 35 min at reduced pressure. after grounding the seeds are mix ... | 1993 | 8237083 |
| sire-1, a long interspersed repetitive dna element from soybean with weak sequence similarity to retrotransposons: initial characterization and partial sequence. | gm776 is a 776-bp subregion of a member of an interspersed family of relatively homogeneous repetitive dna elements from soybean (glycine max). the fragment was originally amplified from soybean dna by the polymerase chain reaction using a single 22-nucleotide primer, and consequently terminates in an inverted repeat. the elements defined by gm776 are at least 10.6 kb in length and constitute a family of 500-800 members per haploid genome. the family has been designated sire-1 (soybean intersper ... | 1993 | 8262373 |
| expression of the soybean (glycine max) glutamate 1-semialdehyde aminotransferase gene in symbiotic root nodules. | extracts of soybean (glycine max) root nodules and greening etiolated leaves catalyzed radiolabeled delta-aminolevulinic acid (ala) formation from 3,4-[3h]glutamate but not from 1-[14c]glutamate. nevertheless, those tissue extracts expressed the activity of glutamate 1-semialdehyde (gsa) aminotransferase, the c5 pathway enzyme that catalyzes ala synthesis from gsa for tetrapyrrole formation. a soybean nodule cdna clone that conferred ala prototrophy, gsa aminotransferase activity, and glutamate- ... | 1993 | 8278535 |
| molecular cloning and expression of 4-coumarate:coenzyme a ligase, an enzyme involved in the resistance response of soybean (glycine max l.) against pathogen attack. | we have isolated three classes of cdnas that probably encode three 4-coumarate:coenzyme a ligase (4cl) isoenzymes in soybean (glycine max l.). the deduced amino acid sequences reveal several regions of extended sequence identity among 4cls of all plants analyzed to date. the sequences of two of these regions are consistent with a domain structure proposed for a group of enzymes catalyzing the atp-dependent covalent binding of amp to their substrates during the reaction sequence. by using two cdn ... | 1993 | 8278545 |