| cloning and characterization of a brassica napus gene encoding a homologue of the b subunit of a heteromeric ccaat-binding factor. | the ccaat motif present in the promoter of several genes is recognized in yeast and animals by a highly specific heteromeric factor (variously called hap, cbf, cp1 or nf-y) which is composed of a minimum of three subunits. a plant homologue of the cbf-b/hap2 subunit is described for the first time in this report. sequence comparison of the brassica napus (bn) ccaat-binding factor (cbf) b subunit with the homologous yeast and animal proteins revealed that the critical amino-acid domains involved ... | 1995 | 8566779 |
| sequence comparison and classification of beet luteovirus isolates. | three distinct sequence groups were found among partial nucleotide sequences of 38 isolates of beet western yellows virus (bwyv) and beet mild yellowing virus (bmyv) from europe, iran and the usa. the first group contains both sugar beet and oilseed rape specific isolates, and the differentiating characteristic linked to this host range specificity are 2 single base pair changes in a 1,200 nucleotide region of the genome. it is proposed that the european bwyv strains that can be transferred at l ... | 1995 | 8572940 |
| characterization of the mitochondrial orfb gene and its derivative, orf224, a chimeric open reading frame specific to one mitochondrial genome of the "polima" male-sterile cytoplasm in rapeseed (brassica napus l.). | orf224 is a novel reading frame present upstream of the atp6 gene in the mitochondria of "polima" cms cytoplasm of rapeseed. in order to determine the origin of orf224, the sequences homologous to orf224 were isolated and characterized. sequence analysis indicated that orf224 originated by recombination events involving the 5'-flanking region and the amino-terminal segment of the coding region of orf158 (well-known as orfb in other plants), part of exon 1 of the ribosomal protein s3 (rps3) gene, ... | 1995 | 8593685 |
| influence of method of administration of rapeseed oil in dairy cows. 2. status of divalent cations. | the effects of method of administration of rapeseed oil on total, soluble, and ionizable ca and mg in ruminal fluid were determined for four diary cows fed a basal diet without (control) or with rapeseed oil (1 kg/d) added by continuous infusion or by a single administration via the ruminal cannula. the control diet contained 68% forage and 32% concentrate mix (dm basis). fatty acid, ca, and mg contents, respectively, were 1.78, 1.16, and .47% for the control diet and 7.38, 1.05, and .44% for th ... | 1995 | 8598408 |
| time-course study of adipose tissue fatty acid composition during mammary tumor growth in rats with controlled fat intake. | previous work in breast cancer patients has indicated an inverse relationship between the risk of relapse and the alpha-linolenic acid (18:3n-3) level in adipose breast tissue. to determine whether low alpha-linolenic levels in patients with aggressive breast cancer resulted from lower 18:3n-3 dietary intake and/or increased metabolism of stored 18:3n-3, we analyzed the fatty acid composition of mammary adipose tissue during tumor growth in a rat model of mammary carcinogenesis. rats were fed a ... | 1995 | 8610049 |
| nucleotide sequence of chinese rape mosaic virus (oilseed rape mosaic virus), a crucifer tobamovirus infectious on arabidopsis thaliana. | the complete nucleotide sequence of chinese rape mosaic virus has been determined. the virus is a member of the tobamovirus genus of plant virus and is able to infect arabidopsis thaliana (l.) heynh systemically. the analysis of the sequence shows a gene array that seems to be characteristic of crucifer tobamoviruses and which is slightly different from the one most frequently found in tobamoviruses. based on gene organization and on comparisons of sequence homologies between members of the toba ... | 1996 | 8616237 |
| cloning of a cdna encoded by a member of the arabidopsis thaliana atp sulfurylase multigene family. expression studies in yeast and in relation to plant sulfur nutrition. | an arabidopsis thaliana atp sulfurylase cdna (asa1), encoding a putative chloroplastic isoform, has been cloned by functional complementation of a saccharomyces cerevisiae (met3) atp sulfurylase mutant which also has a poor sulfate transport capacity. homologous complementation of the yeast mutant with the atp sulfurylase gene restores both atp sulfurylase function and sulfate transport. heterologous complementation restores only atp sulfurylase function as demonstrated by low [35s]sulfate influ ... | 1996 | 8647819 |
| characterization of an acyl-coa-binding protein from arabidopsis thaliana. | a cdna clone was obtained from arabidopsis thaliana that encodes a protein containing 92 amino acid residues with high sequence identity (57%) to bovine acyl-coa-binding protein (acbp). the coding sequence of this clone was expressed in escherichia coli and the gene product (10.4 kda) was purified. the recombinant a. thaliana acbp (rathacbp) was shown to bind acyl-coa esters and protect acyl-coas from degradation by microsomal acyl-hydrolases. antibodies that were raised to rathacbp recognized t ... | 1996 | 8660683 |
| biotin carboxyl carrier protein and carboxyltransferase subunits of the multi-subunit form of acetyl-coa carboxylase from brassica napus: cloning and analysis of expression during oilseed rape embryogenesis. | in the oilseed rape brassica napus there are two forms of acetyl-coa carboxylase (accase). as in other dicotyledonous plants there is a type i accase, the single polypeptide 220 kda form, and a type ii multi-subunit complex analogous to that of escherichia coli and anabaena. this paper describes the cloning and characterization of a plant biotin carboxyl carrier protein (bccp) from the type ii accase complex that shows 61% identity/79% similarity with anabaena bccp at the amino acid level. six c ... | 1996 | 8670092 |
| [transgenic brassica napus resistant to turnip mosaic virus]. | a system for obtaining regenerated plantlets of "double low" brassica napus by using cotyledonary petioles as material was established. the turnip mosaic virus coat protein (tumv-cp) gene inserted in the binary vector pbtu in the agrobacterium tumefaciens lba 4404 was integrated into brassica napus through co-culture of cotyledonary petioles with lba4404, and the material in co-culture was selected under the stress of kanamycin. regenerated plantlets were obtained, and the specific tumv-cp gene ... | 1996 | 8695178 |
| ultrastructure of liver from piglets fed tower rapeseed oil. | effect of tower rapeseed oil (ro) containing lower (0.4%) erucic acid on the piglet liver was studied by electron microscopy. the animals were placed in two groups of four and were fed the diets for eight weeks. animals in the treated group were given a basal diet comprising corn-soybean plus tower ro at a 10% concentration; animals that were given the basal diet with no added oil served as the controls. architecture of the liver from animals of the control group appeared normal. ultrastructural ... | 1996 | 8720445 |
| fats in indian diets and their nutritional and health implications. | to arrive at rat requirements for indians, the contribution of invisible fat should be determined. total lipids were extracted from common indian foods, and their fatty acid compositions were determined. this data and information on intake of various foods were used to estimate the contents of "invisible" fat and fatty acids in indian diets. taking into account world health organization (who) guidelines and the invisible-fat intake of indians, recommendations were made for lower and upper limits ... | 1996 | 8729136 |
| duodenal infusion of oil in midlactation cows. 7. interaction with niacin on responses to glucose, insulin, and beta-agonist challenges. | metabolic effects of rapeseed oil, niacin, or both were investigated for multiparous holstein-friesian cows in midlactation. cows were continuously infused with water (control), rapeseed oil, niacin, or rapeseed oil plus niacin into the proximal duodenum, using a 4 x 4 latin square design. oil infusion resulted in higher plasma concentrations of cholesterol and urea, but a lower plasma concentration of glucose, without changing basal concentrations of insulin, nefa, and bhba in plasma or causing ... | 1995 | 8747336 |
| cloning of brassica napus ctp: phosphocholine cytidylyltransferase cdnas by complementation in a yeast cct mutant. | ctp:phosphocholine cytidylyltransferase is a rate-limiting enzyme in biosynthesis of phosphatidylcholine in plant cells. we have isolated four cdnas for the cytidylyltransferase from a root cdna library of brassica napus by complementation in a yeast cct mutant. the deduced amino-acid sequences of the b. napus enzymes resembled rat and yeast enzymes in the central domain. although all cytidylyltransferases ever cloned from b. napus and other organisms were predicted to be structurally hydrophili ... | 1996 | 8756587 |
| [effect of feeding rapeseed oil to dairy cows on the fatty acid composition of butterfat]. | butter is rich in lauric, myristic, and palmitic acids which are assumed to be hypercholesterolemic. the replacement of usual dietary fat by rapeseed oil induces a serum cholesterol decrease. the objective of the study consisted in measuring the influence of feeding different amounts of full-fat rapeseed or oil-rich rapeseed cake to dairy cows to improve the fatty acid composition of milk fat. the results demonstrate a significant increase of iodine number and spreadability of butter. the percen ... | 1996 | 8766892 |
| identification of a peptide methionine sulphoxide reductase gene in an oleosin promoter from brassica napus. | a bidirectional promoter can be defined operationally as a short segment of dna that regulates divergent transcription. in an attempt to investigate whether the intergenic region between the oleosin and a second open reading frame (orfii) in brassica napus (l.) is a divergent promoter, and also to characterize the orfii, cdna clones homologous to orfii were isolated from a leaf cdna library. a representative cdna (clone d) of one of the two classes identified was identical, in dna sequence, to t ... | 1996 | 8771781 |
| exocrine pancreatic secretions in growing pigs fed diets containing fish oil, rapeseed oil or coconut oil. | two experiments were performed to study the effect of feeding diets containing oils with different fatty acid composition on exocrine pancreatic secretions in growing pigs using two different methods to collect pancreatic juice. in the first experiment, three barrows (initial weight 37 kg) were fitted with a pancreatic pouch re-entrant cannula. an isolated pouch was prepared where the pancreatic duct enters the duodenum. in the second experiment, also using three barrows (initial weight 32 kg), ... | 1996 | 8814194 |
| disruption of an overlapping e-box/abre motif abolished high transcription of the napa storage-protein promoter in transgenic brassica napus seeds. | the storage protein napin is one of the major protein components of brassica napus l. (oilseed rape) seeds. to investigate the transcriptional regulation of the napin promoter, different constructs of the napin gene napa promoter were fused to the escherichia coli uida gene and transformed into b. napus. a-152-bp promoter construct directed a strong expression of the marker gene in mature seeds. the 5' deletion of an additional 8 completely abolished this activity. this deletion disrupted sequen ... | 1996 | 8818291 |
| tissue-specific expression of arabidopsis akt1 gene is consistent with a role in k+ nutrition. | akt1, a putative inwardly directed k+ channel of arabidopsis, restores long-term potassium uptake in a yeast mutant defective in k+ absorption. in this paper, the expression pattern of the gene encoding akt1 is described. northern blots indicate that akt1 transcripts are preferentially accumulated in arabidopsis roots. owing to the difficulties in producing large quantities of arabidopsis roots under hydroponic conditions, experiments were undertaken with brassica napus, a related species. potas ... | 1996 | 8820606 |
| inclusion of oxidized vegetable oil in broiler diets. its influence on nutrient balance and on the antioxidative status of broilers. | over a period of 4 wk, 24 10-d-old broiler hens were fed diets containing 11% vegetable oil (9% rapeseed oil, 2% soybean oil), which was added either fresh (1 meq o2/kg oil) or oxidized (156 meq o2/kg oil). the effects of the dietary treatments on nutrient digestibility were examined in a balance experiment. the antioxidative status of the animals was evaluated using plasma concentrations of thiobarbituric acid-reactive substances (tbars), erythrocyte hemolysis in vitro, selenium-dependent and s ... | 1996 | 8829233 |
| interspecies cross-reactivity of a monoclonal antibody directed against wheat chloroplast fructose-1,6-bisphosphatase. | the primary structure of several chloroplast fructose-1,6-bisphosphatase (cfbpase) was deduced from dna sequences, but only spinach, pea and rapeseed enzymes have been characterized structurally. we analyzed whether cfbpases from different phylogenetic origin contain a common epitope. to this end a dna fragment of 1200 base pairs encoding 338 amino acid residues of wheat cfbpase (38 kda) was cloned in the expression plasmid pgex-1 in frame with the gene coding for glutathione s-transferase (gt) ... | 1996 | 8832098 |
| effects of dietary fatty acid pattern on melting point and composition of adipose tissues and intramuscular fat of broiler carcasses. | soybean oil (so), rapeseed oil (ro), or two commercial fat products (fp1, fp2) were incorporated at 3.5% levels into four different corn-soybean meal mash broiler diets. each of the four diets was fed to five replicates (pens) of broiler chickens for 42 d. after slaughtering the birds, samples of the abdominal fat, subcutaneous fat, and fat extracted from the thigh and the breast portion were collected from 16 birds per treatment. the fat samples were analyzed for their fatty acid composition us ... | 1996 | 8833373 |
| influence of frying fat on the formation of heterocyclic amines in fried beefburgers and pan residues. | the influence of six frying fats (butter, margarine, margarine fat phase, liquid margarine, rapeseed oil and sunflower seed oil) on the formation of mutagenic/carcinogenic heterocyclic amines (has) during the frying of beefburgers was investigated. frying was performed at 165 and 200 degrees c (i.e. under conditions that represented normal household cooking practices). the fried beefburgers and their corresponding pan residues were purified using solid-phase extraction and analysed for has using ... | 1995 | 8847005 |
| effect of rapeseed and dietary oils on the mean survival time of stroke-prone spontaneously hypertensive rats. | low-fat conventional diets supplemented with 5 or 10% vegetable oils were fed to stroke-prone spontaneously hypertensive rats (shr-sp) from weaning and the mean survival times were determined. a 1% aqueous sodium chloride solution was used as drinking water throughout the experiments. in four separate experiments, the rapeseed oil group showed a significantly shorter mean survival time. the relative mean survival times were 50-59% (rapeseed oil group), 78-100% (soybean oil group) and 86% (microb ... | 1996 | 8860957 |
| rhizobium leguminosarum as a plant growth-promoting rhizobacterium: direct growth promotion of canola and lettuce. | early seedling root growth of the nonlegumes canola (brassica campestris cv. tobin, brassica napus cv. westar) and lettuce (lactuca sativa cv. grand rapids) was significantly promoted by inoculation of seeds with certain strains of rhizobium leguminosarum, including nitrogen- and nonnitrogen-fixing derivatives under gnotobiotic conditions. the growth-promotive effect appears to be direct, with possible involvement of the plant growth regulators indole-3-acetic acid and cytokinin. auxotrophic rhi ... | 1996 | 8868235 |
| dietary sunflower oil reduces plasma and liver triacylglycerols in fasting rats and is associated with decreased liver microsomal phosphatidate phosphohydrolase activity. | plasma and liver lipids were studied in male weanling rats fed diets containing moderate levels of fat (6% by weight) as sunflower oil (sf diet, rich in linoleic acid), salmon oil (sm diet, rich in long-chain n-3 fatty acids), or a blend of peanut and rapeseed oil (pr diet, rich in oleic acid). after nine weeks of feeding, the fasting plasma cholesterol concentrations were 49 and 24% lower in groups sm and sf, respectively, as compared to group pr. both dietary salmon oil and sunflower oil lower ... | 1996 | 8869890 |
| fat infiltration in liver of rats induced by different dietary plant oils: high oleic-, medium oleic- and high petroselinic acid-oils. | beneficial effects on health parameters by ingestion of plant oils having high levels of monounsaturated fatty acids such as oleic (cis-9-octadecenoic) acid have been described in several studies. recently, we have shown that feeding of coriander oil containing high proportions of a positional isomer of oleic acid, i.e. petroselinic (cis-6-octadecenoic) acid, led to significantly decreasing proportions of arachidonic (all cis-5,8,11,14-eicosatetraenoic) acid in the cellular lipids. here, we repo ... | 1996 | 8896286 |
| effects of dietary vegetable oils on behavior and drug responses in mice. | previously, we noted significant differences in the behavioral patterns of mice fed safflower oil with a very low alpha-linolenate/linoleate ratio and perilla oil with a high alpha-linolenate/linoleate ratio from mothers to offsprings. in this report, we compared the behavior and drug responses in mice fed diets containing six different vegetable oils-corn, rapeseed, soybean, safflower, perilla and a mixture of perilla and safflower oils- for a relatively short period: 8 months after weaning. so ... | 1996 | 8924909 |
| high-fat diet-induced hyperglycemia and obesity in mice: differential effects of dietary oils. | mice fed a high-fat diet develop hyperglycemia and obesity. using non-insulin-dependent diabetes mellitus (niddm) model mice, we investigated the effects of seven different dietary oils on glucose metabolism: palm oil, which contains mainly 45% palmitic acid (16:0) and 40% oleic acid (18:1); lard oil, 24% palmitic and 44% oleic acid; rapeseed oil, 59% oleic and 20% linoleic acid (18:2); soybean oil, 24% oleic and 54% linoleic acid; safflower oil, 76% linoleic acid; perilla oil, 58% alpha-linolen ... | 1996 | 8969289 |
| castor bean isocitrate lyase lacking the putative peroxisomal targeting signal 1 arm is imported into plant peroxisomes both in vitro and in vivo. | to understand and manipulate plant peroxisomal protein targeting, it is important to establish the universality or otherwise of targeting signals. contradictory results have been published concerning the nature and location of the glyoxysomal/peroxisomal targeting signal of isocitrate lyase (icl). l.j. olsen, w.f. ettinger, b. damsz, k. matsudaira, a. webb, and j.j. harada ([1993] plant cell 5: 941-952) concluded that the last 5 amino acids (aksrm) of brassica napus icl were sufficient and the l ... | 1996 | 8972594 |
| maltophilin: a new antifungal compound produced by stenotrophomonas maltophilia r3089. | strains of stenotrophomonas maltophilia r3089, isolated from the rhizosphere of rape plants (brassica napus l.), produced a novel antifungal compound, named maltophilin, which inhibited the growth of various saprophytic, human-pathogenic and phytopathogenic fungi but was inactive against gram-positive and gram-negative bacteria. maltophilin is a novel macrocyclic lactam antibiotic with a molecular mass of 510 mu. the compound was isolated from the culture filtrate by ethyl acetate extraction and ... | 1996 | 8982338 |
| functional dissection of a napin gene promoter: identification of promoter elements required for embryo and endosperm-specific transcription. | the promoter region (-309 to +44) of the brassica napus storage protein gene napa was studied in transgenic tobacco by successive 5' as well as internal deletions fused to the reporter gene gus (beta-glucuronidase). the expression in the two main tissues of the seed, the endosperm and the embryo, was shown to be differentially regulated. this tissue-specific regulation within the seed was found to affect the developmental expression during seed development. the region between -309 to -152, which ... | 1996 | 9002600 |
| processing in vitro of pronapin, the 2s storage-protein precursor of brassica napus produced in a baculovirus expression system. | the maturation of the 2s albumin, napin, in brassica napus l. involves removal of an amino-terminal and an internal propeptide. pulse-chase experiments with b. napus embryos showed that intermediates are detectable during the pronapin processing. intact pronapin was expressed by baculovirus in spodoptera frugiperda insect cells in order to obtain substrate for studying the processing event. processing of pronapin with a crude b. napus embryo protein extract resulted in several fragments of simil ... | 1996 | 9004547 |
| olive oil and rapeseed oil differ in their effect on plasma low-density lipoprotein metabolism in the guinea-pig. | the effects of olive oil and rapeseed oil, two different high-oleic-acid oils, on plasma ldl and hepatic cholesterol metabolism were compared in guinea-pigs. animals were fed on semipurified diet containing 150 g fat/kg as either olive oil (ol), rapeseed oil plus 100 g palm oil/kg (c-p) or olive oil plus 350 g safflowerseed oil/kg (ol-s). olive oil was enriched with safflowerseed oil (ol-s diet) to increase linoleic acid and to decrease palmitic acid concentrations, in order to evaluate whether ... | 1996 | 9014655 |
| strains of the genus serratia as beneficial rhizobacteria of oilseed rape with antifungal properties. | isolates of serratia have been isolated from the rhizosphere of oilseed rape. the percentage of serratia in this microenvironment was determined as 12.4% of the total antifungal bacteria. serratia liquefaciens, s. plymuthica and s. rubidaea were found. all of the isolates showed an antifungal activity against different phytopathogenic fungi in vitro but the efficiency of strains was different. the antifungal mechanisms of 18 selected strains were investigated. direct antifungal effect may be bas ... | 1996 | 9022304 |
| analysis of the native forms of the 90 kda heat shock protein (hsp90) in plant cytosolic extracts. | a polyclonal antibody, r2, was raised against a fusion protein consisting of a portion of plant hsp90 fused to the trpe protein of escherichia coli. this antibody was found to be specific towards plant hsp90, showing little or no cross-reactivity with mouse and human hsp90 proteins. the r2 antibody identified an 83 kda protein as the hsp90 homologue in cytosolic extracts of several dicot and monocot plants. two-dimensional gel electrophoresis indicated that at least two different isoforms of hsp ... | 1997 | 9049266 |
| reciprocal responses to dietary diacylglycerol of hepatic enzymes of fatty acid synthesis and oxidation in the rat. | the activities of hepatic enzymes of fatty acid synthesis and oxidation were compared in rats fed on diacylglycerol and triacylglycerol. in the first trial, rats were fed on diacylglycerol or triacylglycerol (rapeseed oil) for 14 d. the diacylglycerol preparation contained 65.2 g and 32.6 g fatty acids/100 g total fatty acids as 1,3-species and 1,2-species respectively. fatty acid compositions of these dietary lipids were similar. dietary acylglycerols were added to experimental diets to provide ... | 1997 | 9059234 |
| oilseed rape pollen is a potentially relevant allergen. | oilseed rape (brassica napus) (osr) is a partly wind-pollinated crop which has been increasingly cultivated both in europe and overseas. allergological data about osr is scarce and controversial. we evaluated the frequency of sensitization to osr pollen by skin prick test and rast over a period of 1 yr. airborne osr pollen load and the agricultural role of this crop were analysed. furthermore, six patients were investigated by immunoblot. in 4468 patients with suspect inhalant allergy investigat ... | 1997 | 9061214 |
| oilseed isocitrate lyases lacking their essential type 1 peroxisomal targeting signal are piggybacked to glyoxysomes. | isocitrate lyase (il) is an essential enzyme in the glyoxylate cycle, which is a pathway involved in the mobilization of stored lipids during postgerminative growth of oil-rich seedlings. we determined experimentally the necessary and sufficient peroxisome targeting signals (ptss) for cottonseed, oilseed rape, and castor bean ils in a well-characterized in vivo import system, namely, suspension-cultured tobacco (bright yellow) by-2 cells. results were obtained by comparing immunofluorescence loc ... | 1997 | 9061950 |
| study of apoptosis in human lymphocytes by toxic substances implicated in toxic oil syndrome. | toxic oil syndrome is a multisystemic disease that occurred in epidemic proportions in spain in 1981 caused by the ingestion of rapeseed oil denatured with aniline. several data implicate t cells in the pathogenesis of the disease. we evaluated the mechanisms of cytotoxicity in human lymphocytes of tos-related products: aniline, 3-(n-phenylamino)-1,2-propanediol and its mono- and di-oleyl esters and eosinophilia myalgia-related product such as 3-(phenylamino)-l-alanine, which is chemically simil ... | 1997 | 9074655 |
| the effect of dietary oil containing (n-3) fatty acids on the fatty acid, physicochemical, and organoleptic characteristics of pig meat and fat. | an investigation was made to alter the fatty acid composition of pork and a pork product in line with human dietary advice while not adversely affecting factors controlling consumer acceptability. pigs (n = 150) were assigned to three dietary treatments with 25 intact male-female pairs per treatment. diet a (control) contained 3% of a 4:1 (wt/ wt) tallow-soybean oil mixture. diets b and c contained 2% rapeseed oil plus 1% fish oil. diets a, b, and c were supplemented with 100, 100, and 250 mg of ... | 1997 | 9078483 |
| a cysteine endopeptidase isolated from castor bean endosperm microbodies processes the glyoxysomal malate dehydrogenase precursor protein. | a plant cysteine endopeptidase with a molecular mass of 35 kd was purified from microbodies of germinating castor bean (ricinus communis) endosperm by virtue of its capacity to specifically process the glyoxysomal malate dehydrogenase precursor protein to the mature subunit in vitro. processing of the glyoxysomal malate dehydrogenase precursor occurs sequentially in three steps, the first intermediate resulting from cleavage after arginine-13 within the presequence and the second from cleavage a ... | 1997 | 9085576 |
| accumulation of ricinoleic, lesquerolic, and densipolic acids in seeds of transgenic arabidopsis plants that express a fatty acyl hydroxylase cdna from castor bean. | a cdna encoding the oleate 12-hydroxylase from castor bean (ricinus communis l.) has previously been shown to direct the synthesis of small amounts of ricinoleic acid (12-hydroxyoctadec-cis-9-enoic acid) in seeds of transgenic tobacco plants. expression of the cdna under control of the brassica napus napin promoter in transgenic arabidopsis thaliana plants resulted in the accumulation of up to 17% of seed fatty acids as ricinoleate and two novel fatty acids that have been identified by gas chrom ... | 1997 | 9085577 |
| effects of seed-specific expression of a cytokinin biosynthetic gene on canola and tobacco phenotypes. | the agrobacterium tumefaciens isopentenyl transferase gene (ipt), a cytokinin biosynthetic gene, was placed under the control of 1.9 kb of promoter sequence from the 2s albumin at2s1 gene isolated from an arabidopsis thaliana library. the construct was introduced into canola (brassica napus) and tobacco (nicotiana tabacum). ipt transcripts were followed during embryo development of transgenic plants by northern hybridizations. the phenotype of transformed plants from the t1 generation was analys ... | 1997 | 9090061 |
| multi-functional acetyl-coa carboxylase from brassica napus is encoded by a multi-gene family: indication for plastidic localization of at least one isoform. | three genes coding for different multifunctional acetyl-coa carboxylase (accase; ec 6.4.1.2) isoenzymes from brassica napus were isolated and divided into two major classes according to structural features in their 5' regions: class i comprises two genes with an additional coding exon of approximately 300 bp at the 5' end, and class ii is represented by one gene carrying an intron of 586 bp in its 5' untranslated region. fusion of the peptide sequence encoded by the additional first exon of a cl ... | 1997 | 9096417 |
| protein kinase c activation in rat colonic mucosa after diets differing in their fatty acid composition. | we studied the effects of different types of dietary fats on fatty acid composition and activity of protein kinase c (pkc) in rat colonic mucosa. activation of pkc, a key enzyme in signal transduction and growth regulation, provides a mechanism by which dietary components could be involved in colon carcinogenesis. male wistar rats (n = 12/group) were fed a semisynthetic high fat diet (43% of energy) containing either sunflower oil, rapeseed oil, or butter for 4 weeks ad libitum. the control grou ... | 1997 | 9103264 |
| brassica napus cdnas encoding fatty acyl-coa synthetase. | from a cdna library of developing siliques of rapeseed (brassica napus l.) we have isolated five full-length clones encoding polypeptides of the amp-binding protein family. two cdnas encode fatty acyl-coa synthetase activity (ec 6.2.1.3). the deduced polypeptides share about 52% identical amino acids. after expression in escherichia coli the predicted enzymatic activity was confirmed by in vitro assays and product analysis. the enzymatic activity for one of the clones was characterized in detail ... | 1997 | 9106514 |
| characterization of cysteine residues involved in the reductive activation and the structural stability of rapeseed (brassica napus) chloroplast fructose-1,6-bisphosphatase. | in higher plants, light enhances the activity of chloroplast fructose-1,6-bisphosphatase via a cascade of thiol/disulfide exchanges. we have examined the structural and functional role of seven conserved cysteine residues in the rapeseed (brassica napus) enzyme by site-directed mutagenesis. after lysis of escherichia coli cells, c53s and c191s variants partitioned mainly in the insoluble fraction whereas c96s, c157s, c174s, c179s, and c307s mutants were soluble. homogeneous preparations of the l ... | 1997 | 9125187 |
| insulin-induced maturation of xenopus oocytes is inhibited by microinjection of a brassica napus cdna clone with high similarity to a mammalian receptor for activated protein kinase c. | a cdna clone encoding a wd-40 repeat protein (bgb1) was characterized in brassica napus l. the clone contained an open reading frame of 327 amino acid residues almost entirely composed of seven segments of wd-40 repeats. among the wd-40 repeat proteins, bgb1 showed high similarity (63% identity) to a rat intracellular receptor for protein kinase c (rack1) that functions in the translocation of activated protein kinase c (pkc) from the cytosolic fraction to the membrane fraction. bgb1 also had tw ... | 1997 | 9129334 |
| effect of dietary fats rich in lauric, myristic, palmitic, oleic or linoleic acid on plasma, hepatic and biliary lipids in cholesterol-fed hamsters. | effects of different dietary fats on plasma, hepatic and biliary lipids were determined in male golden syrian hamsters (mesocricetus auratus) fed on purified diets for 7 weeks. diets were made by blending different fats containing characteristic fatty acids: butter (14:0 + 16:0), palm stearin (16:0), coconut oil (12:0 + 14:0), rapeseed oil (18:1), olive oil (18:1) and sunflowerseed oil (18:2). in all diets except the sunflowerseed oil diet dietary 18:2 was held constant at 2% energy. total fat s ... | 1997 | 9155509 |
| polyunsaturated fatty acid deficiencies: effects on hepatic plasma membrane fatty acid composition and enzyme activity. | research on dietary polyunsaturated fatty acids (pufa), on the activity of 5'nucleotidase and adenylate cyclase are largely contradictory due, mostly, to the absence of adequate control group. in this study; four different diets have been evaluated on the 5'nucleotidase and adenylate cyclase activities in rat liver plasma membranes. wistar rats were given a semisynthetic diet in which lipids were supplied by 5% of either peanut oil (n-3 pufa deficient diet), cod liver oil (n-6 pufa deficient die ... | 1995 | 9161445 |
| effects of dietary calcium soaps of unsaturated fatty acids on digestion, milk composition and physical properties of butter. | dairy cows fitted with ruminal, duodenal and ileal cannulas were utilized to investigate the effects of feeding with ca soaps (cas) of palm fatty acids (fa) and rapeseed fa. diets compared were control diet based on maize silage and concentrate, and two diets with 40 g cas of palm oil fa or rapeseed oil fa/kg diet, replacing part of the concentrates of the control diet. total digestibilities of dry matter, fibre and fat, and ruminal fermentation were not significantly altered by giving cas; the ... | 1997 | 9161912 |
| di-fluoresceinthiocarbamyl-insulin: a fluorescent substrate for the assay of protein disulfide oxidoreductase activity. | we have developed a novel method for the continuous assay of protein disulfide oxidoreductase activity using as substrate bovine pancreas insulin in which both n-terminal amino groups are chemically modified with fluorescein isothiocyanate. the reduction of intercatenary disulfide bonds of di-fluoresceinthiocarbamyl-insulin with dithiothreitol initially lowers but subsequently enhances the emission intensity. in this biphasic kinetics, the rate of increase is sensitive enough for the estimation ... | 1997 | 9177728 |
| broad-range and binary-range acyl-acyl-carrier protein thioesterases suggest an alternative mechanism for medium-chain production in seeds. | in the current model of medium-chain (c8-14) fatty acid biosynthesis in seeds, specialized fatb acyl-acyl-carrier-protein (acp) thioesterases are responsible for the production of medium chains. we have isolated and characterized fatb cdnas from the maturing seeds of elm (ulmus americana) and nutmeg (myristica fragrans), which accumulate predominantly caprate (10:0)- and myristate (14:0)-containing oils, respectively. in neither species were we able to find cdnas encoding enzymes specialized for ... | 1997 | 9193098 |
| modification of seed oil content and acyl composition in the brassicaceae by expression of a yeast sn-2 acyltransferase gene. | a putative yeast sn-2 acyltransferase gene (slc1-1), reportedly a variant acyltransferase that suppresses a genetic defect in sphingolipid long-chain base biosynthesis, has been expressed in a yeast slc deletion strain. the slc1-1 gene product was shown in vitro to encode an sn-2 acyltransferase capable of acylating sn-1 oleoyl-lysophosphatidic acid, using a range of acyl-coa thioesters, including 18:1-, 22:1-, and 24:0-coas. the slc1-1 gene was introduced into arabidopsis and a high erucic acid ... | 1997 | 9212466 |
| molecular genetic and biochemical analysis of brassica napus proliferating cell nuclear antigen function. | a cdna encoding the proliferating cell nuclear antigen (pcna) from brassica napus (oilseed rape) was shown to complement the lethal deletion mutation in the pcna gene (delta pol30) of saccharomyces cerevisiae. we provide unequivocal evidence that the b. napus pcna can perform all the essential functions of the yeast pcna in dna replication, although some species-specific differences may exist. in addition, the b. napus pcna expressed as a fusion polypeptide with glutathione s-transferase (gst) w ... | 1997 | 9247551 |
| unusual effects of some vegetable oils on the survival time of stroke-prone spontaneously hypertensive rats. | preliminary experiments have shown that a diet containing 10% rapeseed oil (low-erucic acid) markedly shortens the survival time of stroke-prone spontaneously hypertensive (shrsp) rats under 1% nacl loading as compared with diets containing perilla oil or soybean oil. high-oleate safflower oil and high-oleate sunflower oil were found to have survival time-shortening activities comparable to that of rapeseed oil; olive oil had slightly less activity. a mixture was made of soybean oil, perilla oil ... | 1997 | 9252963 |
| the glyoxysomal 3-ketoacyl-coa thiolase precursor from brassica napus has enzymatic activity when synthesized in escherichia coli. | glyoxysomal 3-ketoacyl-coa thiolase is the last enzyme in the beta-oxidation of fatty acids in plant glyoxysomes. a full-length cdna of the glyoxysomal 3-ketoacyl-coa thiolase from brassica napus and a truncated version, lacking the n-terminal targeting signal were cloned in a t7 promoter-based vector. both recombinant proteins were expressed in escherichia coli and activity was measured. full-length and truncated 3-ketoacyl-coa thiolase have comparable activity in e. coli. moreover, full-length ... | 1997 | 9257706 |
| recombinant antibody fragments that detect enoyl acyl carrier protein reductase in brassica napus. | purified brassica napus enoyl acyl carrier protein reductase (enr) was used to select specific antibodies from a library of antibody fragments, single-chain fv (scfv), displayed on filamentous phage. analysis of the selected clones by bstni fingerprinting and nucleotide sequencing showed that the scfv were derived from three different human vh germline genes. the binding specificities were confirmed by western blots and elisa. the scfv preparations reacted with b. napus enr, but not with beta-ke ... | 1997 | 9270971 |
| nutritional evaluation of low glucosinolate mustard meals (brassica juncea) in broiler diets. | experiments were conducted to evaluate the nutritional value of meal derived from low glucosinolate cultivars of mustard (brassica juncea) in comparison to samples of canola meal (brassica napus, brassica rapa). samples of brassica seed (four b. juncea, one b. napus, and one b. rapa) were processed using laboratory procedures to produce oil-extracted meals, which were examined for composition (dm basis), and nutritional value for broiler chickens as judged by nutrient retention (amen, ileal prot ... | 1997 | 9276890 |
| breast milk fatty acid composition: a comparative study between hong kong and chongqing chinese. | the fatty acids of milk samples obtained from 51 hong kong chinese and 33 chongqing chinese (si chuan province, china) were analyzed by gas-liquid chromatography. compared with those of published data for canadian and other western countries, the chinese milk from both hong kong and chongqing contained higher levels of longer-chain polyunsaturated fatty acids, particularly docosahexaenoic acid (22:6n-3) and arachidonic acid (20:4n-6). in contrast, the content of trans fatty acids in the chinese ... | 1997 | 9358432 |
| identification of a ca2+ binding protein as a new bermuda grass pollen allergen cyn d 7: ige cross-reactivity with oilseed rape pollen allergen bra r 1. | cdna clones encoding two isoforms of an allergen from pollen of bermuda grass (cynodon dactylon) have been isolated using ige from allergic patients. homologous transcripts are present in pollen of 15 other grasses tested. this allergen, tentatively designated as cyn d 7, contains two calcium binding domains and shows significant sequence similarity with other ca2+ binding pollen allergens, namely bet v 4 from birch and bra r 1 from oilseed rape. approximately 10% of allergic sera tested showed ... | 1997 | 9363908 |
| genome analysis of the fungal plant pathogen, leptosphaeria maculans using pulsed field gel electrophoresis. | pulsed field gel electrophoresis (pfge), or electrophoretic karyotyping, separates chromosomal-sized pieces of dna in agarose gels where the orientation of the electric field is periodically altered. this technique has revealed that many fungi have a high degree of chromosomal length polymorphisms. often the only isolates with identical karyotypes are derived from a single clone, thus pfge provides a 'genetic fingerprint' for them. the size range and number of chromosomes within isolates of a pa ... | 1997 | 9378119 |
| a functional s locus anther gene is not required for the self-incompatibility response in brassica oleracea. | the self-incompatibility (si) response in brassica involves recognition of self-pollen by the papillar cells of the stigma and is mediated by the products of genes localized at the s (self-incompatibility) locus. two s locus genes, srk and slg, are thought to encode components of a receptor complex present in the female partner. the putative gene product of sla, a third s locus-linked gene that is expressed specifically in anthers, is a candidate for the male component of the si recognition syst ... | 1997 | 9401128 |
| plant s1 sines as a model to study retroposition. | the s1 element is a plant sine (short interspersed element) that was first described and studied in brassica napus and is widely distributed among cruciferae, especially in species of the brassiceae tribe. we propose that s1 amplification in cruciferae could represent a good eukaryotic model to study retroposition. this is based on the fact that s1 elements share clear structural and evolutionary characteristics with mammalian sines but are present in a much lower copy number (500 loci by haploi ... | 1997 | 9440268 |
| strain-dependent cytokine profile and susceptibility to oleic acid anilide in a murine model of the toxic oil syndrome. | the toxic oil syndrome (tos) was caused by the ingestion of an adulterated rapeseed oil containing oleic acid anilide (oaa). it was characterized by lethal symptoms in the acute phase and by symptoms of idiopathic autoimmune diseases in the chronic phase. the pathogenetic mechanisms remain unclear. in a murine model of tos we demonstrate strain-dependent effects on the immune system after treatment with oaa intraperitoneally. while c57bl/6 (h-2b) mice develop a polyclonal b cell activation witho ... | 1998 | 9473529 |
| docosahexaenoic acid concentrations in retinal phospholipids of piglets fed an infant formula enriched with long-chain polyunsaturated fatty acids: effects of egg phospholipids and fish oils with different ratios of eicosapentaenoic acid to docosahexaenoic acid. | docosahexaenoic acid (dha; 22:6n-3) is the major fatty acid in the phosphatidylethanolamine of photoreceptor cells. the supply of preformed dha in milk may play an important role in early human visual development. we examined the effect of adding dietary dha from yolk or fish oil on its accretion in the retina of newborn piglets fed artificially for 2 wk. dha-enriched eggs from hens fed rapeseed oil and two fish oils with a high or low ratio of eicosapentaenoic acid (epa; 20:5n-3) to dha were us ... | 1998 | 9497179 |
| collinearity between a 30-centimorgan segment of arabidopsis thaliana chromosome 4 and duplicated regions within the brassica napus genome. | arabidopsis thaliana (the model dicotyledonous plant) is closely related to brassica crop species. genome collinearity, or conservation of marker order, between brassica napus (oilseed rape) and a. thaliana was assessed over a 7.5-mbp region of the long arm of a. thaliana chromosome 4, equivalent to 30 cm. estimates of copy number indicated that sequences present in a single copy in the haploid genome of a. thaliana (n = 5) were present in 2-8 copies in the haploid genome of b. napus (n = 19), w ... | 1998 | 9549059 |
| chronic dietary n-3 polyunsaturated fatty acids deficiency affects the fatty acid composition of plasmenylethanolamine and phosphatidylethanolamine differently in rat frontal cortex, striatum, and cerebellum. | as chronic consumption of a diet devoid of n-3 fatty acid induced modification of neurotransmission pathways in the frontal cortex of rats, plasmalogen alteration could occur in this area. because of the propensity to facilitate membrane fusion, plasmenylethanolamine (pme), a major plasmalogen of brain, may be involved in synaptic transmission. female rats were fed diet containing peanut oil [(n-3)-deficient diet] through two generations. two weeks before mating, half of the female rats of the s ... | 1998 | 9590628 |
| detection of nptii (kanamycin resistance) genes in genomes of transgenic plants by marker-rescue transformation. | we have developed a novel system for the sensitive detection of nptii genes (kanamycin resistance determinants) including those present in transgenic plant genomes. the assay is based on the recombinational repair of an nptii gene with an internal 10-bp deletion located on a plasmid downstream of a bacterial promoter. uptake of an nptii gene by transformation restores kanamycin resistance. in escherichia coli, promoterless nptii genes provided by electroporation were rescued with high efficiency ... | 1998 | 9604883 |
| isolation, characterisation and expression of a cdna for pea cholinephosphate cytidylyltransferase. | in plants, phosphatidylcholine is the major phospholipid in extra-plastid membranes and is synthesised mainly by the cdp-choline pathway. evidence from studies in animals, as well as in plants, suggests that the intermediate step catalysed by cholinephosphate cytidylyltransferase (cpct) has a major control in carbon flux to this lipid. we have isolated a full-length cpct cdna (designated pct2) from pisum sativum cv. feltham first using an arabidopsis probe and the polymerase chain reaction (pcr) ... | 1998 | 9620275 |
| field tolerance to fungal pathogens of brassica napus constitutively expressing a chimeric chitinase gene. | constitutive overexpression of a protein involved in plant defense mechanisms to disease is one of the strategies proposed to increase plant tolerance to fungal pathogens. a hybrid endochitinase gene under a constitutive promoter was introduced by agrobacterium-mediated transformation into a winter-type oilseed rape (brassica napus var. oleifera) inbred line. progeny from transformed plants was challenged using three different fungal pathogens (cylindrosporium concentricum, phoma lingam, sclerot ... | 1996 | 9630959 |
| the role of acyl carrier protein isoforms from cuphea lanceolata seeds in the de-novo biosynthesis of medium-chain fatty acids. | to investigate the role of acyl carrier protein (acp) in determining the fate of the acyl moieties linked to it in the course of de-novo fatty acid biosynthesis in higher plants, we carried out in vitro experiments to reconstitute the fatty acid synthase (fas) reaction in extracts of spinach (spinacia oleracea l.) leaves, rape (brassica napus l.) seeds and cuphea lanceolata ait. seeds. the action of two major c. lanceolata acp isoforms (acp 1 and acp 2) compared to acp from escherichia coli was ... | 1998 | 9637071 |
| can adults adequately convert alpha-linolenic acid (18:3n-3) to eicosapentaenoic acid (20:5n-3) and docosahexaenoic acid (22:6n-3)? | a diet including 2-3 portions of fatty fish per week, which corresponds to the intake of 1.25 g epa (20:5n-3) + dha (22:6n-3) per day, has been officially recommended on the basis of epidemiological findings showing a beneficial role of these n-3 long-chain pufa in the prevention of cardiovascular and inflammatory diseases. the parent fatty acid ala (18:3n-3), found in vegetable oils such as flaxseed or rapeseed oil, is used by the human organism partly as a source of energy, partly as a precurs ... | 1998 | 9637947 |
| effects of rapessed oil on activity of methylmalonyl-coa carboxyltransferase in culture of streptomyces fradiae. | to investigate why more tylosin was produced when streptomyces fradiae t1558 was cultured in a rapeseed oil medium than in a glucose or starch medium, we measured the activity of methylmalonyl-coa carboxyltransferase (ec 2.1.3.1) and intracellular propionic acid. the activity of the enzyme, which catalyzes the formation of the precursor of tylosin, protylonolide, was 0.19 u/mg protein in 5 days of culture in rapeseed oil medium, which was 2.5- and 1.3-fold that with the glucose or starch medium, ... | 1998 | 9648221 |
| biosynthesis, targeting and processing of oleosin-like proteins, which are major pollen coat components in brassica napus. | the purpose of this study is to characterise the biosynthesis, targeting and processing of some of the major protein components of the pollen coat, or tryphine, of brassica napus. the authors have n-terminally sequenced 11 of the most abundant pollen coat polypeptides, and nine of these sequences correspond to proteolytically cleaved products of seven oleosin-like genes, i.e. oln b;1 to oln b;6 and oln b;11. the oln b;11 gene product is co- or post-translationally targeted in vitro to canine mic ... | 1998 | 9680961 |
| promoter regions of the exta extensin gene from brassica napus control activation in response to wounding and tensile stress. | to identify controlling cis acting promoter regions in the b. napus exta extensin gene, expression in transgenic tobacco of 5' - 159, -433, -664, -789 and -940 bp promoter truncations linked to the uida (b-glucuronidase) reporter coding sequence were analysed. the - 159 and -433 bp truncations directed non specific expression in all cell types within the plant. an activator region which increased expression levels 10 fold in all cell types was located between - 159 to -433 bp. a repressor region ... | 1998 | 9687071 |
| free fatty acid fractions from some vegetable oils exhibit reduced survival time-shortening activity in stroke-prone spontaneously hypertensive rats. | previously, we demonstrated that several vegetable oils that included low-erucic rapeseed oil markedly shortened the survival time (by approximately 40%) of stroke-prone spontaneously hypertensive (shrsp) rats as compared with perilla oil, soybean oil, and fish oil. we considered that a factor other than fatty acids is toxic to shrsp rats, because the survival time-shortening activity could not be accounted for by the fatty acid compositions of these oils. in fact, a free fatty acid (ffa) fracti ... | 1998 | 9688167 |
| a brassica cdna clone encoding a bifunctional hydroxymethylpyrimidine kinase/thiamin-phosphate pyrophosphorylase involved in thiamin biosynthesis. | we report the characterization of a brassica napus cdna clone (pbthi) encoding a protein (bthi) with two enzymatic activities in the thiamin biosynthetic pathway, thiamin-phosphate pyrophosphorylase (tmp-ppase) and 2-methyl-4-amino-5-hydroxymethylpyrimidine-monophosphate kinase (hmp-p kinase). the cdna clone was isolated by a novel functional complementation strategy employing an escherichia coli mutant deficient in the tmp-ppase activity. a biochemical assay showed the clone to confer recovery ... | 1998 | 9700068 |
| mutagenic and cytotoxic effects of exhaust particulate matter of biodiesel compared to fossil diesel fuel. | the mutagenic and cytotoxic effects of diesel engine exhaust (dee) from a modern passenger car using rapeseed oil methyl esters (rme, biodiesel) as fuel were directly compared to dee of diesel fuel (df) derived from petroleum. combustion particulate matter was collected on glass fiber filters coated with polytetrafluoroethylene (ptfe) from an exhaust dilution tunnel using three different engine test cycles on a chassis dynamometer. filters were extracted with dichloromethane in a soxhlet apparat ... | 1998 | 9711258 |
| [asthma and rhinoconjunctivitis caused by rape flour: description of a clinical case]. | a 48 year old man, employed in a grain and animal feed store for 9 years, was referred to our clinic complaining of nasal blockage, rhinorrhea, sneezing, ocular burning, coughing and wheezing occurring over the last 12 months. the man's main task was to manually load and unload the unpackaged grain and feed. symptoms occurred only when he worked directly with oilseed rape flour and not when he worked with other types of grains. eye and nasal symptoms appeared during the work shift, while respira ... | 1998 | 9734192 |
| brassica napus hsp90 can autophosphorylate and phosphorylate other protein substrates. | a brassica napus cdna encoding the 90 kda heat shock protein, hsp90, was modified to add 6 histidines at the c-terminus and expressed in insect cells to prepare a recombinant histidine-tagged hsp90. the recombinant protein was purified over ni2+-nta agarose columns and its identity was confirmed by western blotting, using a plant hsp90-specific antiserum. incubation of purified hsp90 with [gamma-32p] atp in the presence of mn2+ resulted in its autophosphorylation on serine residues. the purified ... | 1998 | 9746209 |
| cloning and characterization of cold-regulated glycine-rich rna-binding protein genes from leafy spurge (euphorbia esula l.) and comparison to heterologous genomic clones. | leafy spurge (euphorbia esula) is a perennial weed which is capable of acclimating to sub-freezing temperatures. we have used the differential display technique to identify and clone a cdna for a cold-regulated gene (cor20) which hybridizes to mrnas that accumulate specifically during the cold acclamation process. the cor20 cdna was used to isolate two different genomic clones. both clones were similar but not identical to each other and the cdna. sequence analysis of the genomic clones indicate ... | 1998 | 9747799 |
| oilseed rape allergy presented as occupational asthma in the grain industry. | there have been several reports on respiratory allergic symptoms induced by pollen of oilseed rape. to the best of our knowledge, this is the first report dealing with oilseed rape dust mainly composed of seeds, as an occupational allergen in the grain industry. in this paper, we present a case of occupational asthma caused by oilseed rape dust from the animal feed industry, which proved to be induced by an ige-mediated reaction. | 1998 | 9761021 |
| cdna cloning of brassica napus malonyl-coa:acp transacylase (mcat) (fab d) and complementation of an e. coli mcat mutant. | the genbank database was searched using the e. coli malonyl coa:acp transacylase (mcat) sequence, for plant protein/cdna sequences corresponding to mcat, a component of plant fatty acid synthetase (fas), for which the plant cdna has not been isolated. a 272-bp zea mays est sequence (genbank accession number: aa030706) was identified which has strong homology to the e. coli mcat. a pcr derived cdna probe from zea mays was used to screen a brassica napus (rape) cdna library. this resulted in the i ... | 1998 | 9762909 |
| identification of allergens in oilseed rape (brassica napus) pollen. | pollen from oilseed rape (osr), brassica napus, an increasingly cultivated oilplant from the brassicaceae, has been recognized as a potential cause of allergic sensitization. allergens have been hardly investigated. | 1998 | 9784653 |
| a sphingolipid desaturase from higher plants. identification of a new cytochrome b5 fusion protein. | a recently cloned cdna from sunflower codes for a fusion protein composed of an n-terminal cytochrome b5 and a domain similar to membrane-bound acyl lipid desaturases. for a functional identification, homologous cdnas from brassica napus and arabidopsis thaliana were expressed in saccharomyces cerevisiae, and sphingolipid long chain bases were analyzed. the expression of the heterologous enzyme results in significant proportions of new delta8, 9-cis/trans-phytosphingenines that accompany the res ... | 1998 | 9786850 |
| nucleotide sequence analysis of the 3' terminal region of a wasabi strain of crucifer tobamovirus genomic rna: subgrouping of crucifer tobamoviruses. | the 3' terminal 2378 nucleotides of a wasabi strain of crucifer tobamovirus (ctmv-w) infectious to crucifer plants was determined. this includes the 3' non-coding region of 235 nucleotides, coat protein (cp) gene (468 nucleotides), movement protein (mp) gene (798 nucleotides) and c-terminal partial readthrough portion of 180 k protein gene (940 nucleotides). comparison of the sequence with homologous regions of thirteen other tobamovirus genomes showed that it had much higher identity to those o ... | 1998 | 9787663 |
| an evolutionary conserved group of plant gsk-3/shaggy-like protein kinase genes preferentially expressed in developing pollen. | genes and cdnas encoding plant protein kinases highly homologous to the animal gsk-3/shaggy subfamily were isolated from arabidopsis thaliana, brassica napus, petunia hybrida and nicotiana tabacum using the p. hybrida psk6 gsk-3/shaggy related cdna as a probe. all the derived protein sequences contained the characteristic catalytic domain of gsk-3/shaggy protein kinases. sequence comparisons within the catalytic domain with other plant gsk-3/shaggy like kinases clearly indicate that the novel se ... | 1998 | 9804971 |
| synthesis of medium-chain-length polyhydroxyalkanoates in arabidopsis thaliana using intermediates of peroxisomal fatty acid beta-oxidation. | polyhydroxyalkanoate (pha) is a family of polymers composed primarily of r-3-hydroxyalkanoic acids. these polymers have properties of biodegradable thermoplastics and elastomers. medium-chain-length phas (mcl-phas) are synthesized in bacteria by using intermediates of the beta-oxidation of alkanoic acids. to assess the feasibility of producing mcl-phas in plants, arabidopsis thaliana was transformed with the phac1 synthase from pseudomonas aeruginosa modified for peroxisome targeting by addition ... | 1998 | 9811811 |
| differential expression of a senescence-enhanced metallothionein gene in arabidopsis in response to isolates of peronospora parasitica and pseudomonas syringae. | the metallothionein gene, lsc54, shows increased expression during leaf senescence in brassica napus and arabidopsis thaliana. a number of abiotic and biotic stresses have been shown to induce senescence-like symptoms in plants and, to investigate this further, the promoter of the lsc54 gene was cloned and fused to the gus gene and transformed into arabidopsis. the promoter was highly induced during leaf senescence and also in response to wounding; histochemical analysis indicated that this indu ... | 1998 | 9839466 |
| independent deletions of a pathogen-resistance gene in brassica and arabidopsis. | plant disease resistance (r) genes confer race-specific resistance to pathogens and are genetically defined on the basis of intra-specific functional polymorphism. little is known about the evolutionary mechanisms that generate this polymorphism. most r loci examined to date contain alternate alleles and/or linked homologs even in disease-susceptible plant genotypes. in contrast, the resistance to pseudomonas syringae pathovar maculicola (rpm1) bacterial resistance gene is completely absent (rpm ... | 1998 | 9861058 |
| the presence of cyp79 homologues in glucosinolate-producing plants shows evolutionary conservation of the enzymes in the conversion of amino acid to aldoxime in the biosynthesis of cyanogenic glucosides and glucosinolates. | a cdna encoding cyp79b1 has been isolated from sinapis alba. cyp79b1 from s. alba shows 54% sequence identity and 73% similarity to sorghum cyp79a1 and 95% sequence identity to the arabidopsis t42902, assigned cyp79b2. the high identity and similarity to sorghum cyp79a1, which catalyses the conversion of tyrosine to p-hydroxyphenylacetaldoxime in the biosynthesis of the cyanogenic glucoside dhurrin, suggests that cyp79b1 similarly catalyses the conversion of amino acid(s) to aldoxime(s) in the b ... | 1998 | 9862490 |
| dietary fish oil affects monoaminergic neurotransmission and behavior in rats. | we studied the effects of a fish oil enriched diet on fatty acid composition of cerebral membranes and on several neurochemical and behavioral variables of monoaminergic function in rats. the frontal cortex, striatum, hippocampus and cerebellum were studied in rats fed fish oil (fpo, 50% salmon oil + 50% palm oil), which provided an (n-6)/(n-3) polyunsaturated fatty acid (pufa) ratio of 0.14 versus 6. 19 in controls fed a diet containing a mixture of african peanut oil and rapeseed oil. in the f ... | 1998 | 9868201 |
| elevation of conjugated cis-9, trans-11-octadecadienoic acid in bovine milk because of dietary supplementation. | cows on pasture were fed full fat soybeans (toasted, flaked, and pelleted) or ground full fat rapeseeds to investigate effects on cis-9, trans-11-octadecadienoic acid in milk. three herds of 16 cows each that were on pasture were fed 3.1 kg/d of unmolassed beet pulp (control), 3.0 kg/d of rapeseed concentrate, or 3.1 kg/d of a soybean supplement. the concentration of cis-9, trans-11-octadecadienoic acid in the milk of cows fed the rapeseed and soybean supplements was significantly higher than in ... | 1998 | 9891271 |
| molecular characterisation of plant cdnas bnmap4kalpha1 and bnmap4kalpha2 belonging to the gck/sps1 subfamily of map kinase kinase kinase kinase. | several yeast and mammal map kinase modules require, upstream of their map kinase kinase kinase (map3k), a map3k kinase (map4k). an arabidopsis thaliana est clone, sharing identity to map4ks from yeast and mammals, has been used to isolate cdna clones from a brassica napus microspore-derived embryo cdna library. the bnmap4kalpha1 and bnmap4k-alpha2 clones encode putative proteins possessing the 12 subdomains of the serine/threonine protein kinase catalytic domain. a detailed analysis showed that ... | 1999 | 9931402 |
| structure, properties, and tissue localization of apoplastic alpha-glucosidase in crucifers. | apoplastic alpha-glucosidases occur widely in plants but their function is unknown because appropriate substrates in the apoplast have not been identified. arabidopsis contains at least three alpha-glucosidase genes; aglu-1 and aglu-3 are sequenced and aglu-2 is known from six expressed sequence tags. antibodies raised to a portion of aglu-1 expressed in escherichia coli recognize two proteins of 96 and 81 kd, respectively, in vegetative tissues of arabidopsis, broccoli (brassica oleracea l.), a ... | 1999 | 9952433 |
| antioxidative and oxidative status in muscles of pigs fed rapeseed oil, vitamin e, and copper. | the susceptibility of a given muscle tissue to lipid oxidation may not only depend on the presence of unsaturated fatty acids and the balance between antioxidants and prooxidants, but also on the composition of the skeletal muscle. in the present study, the effects of dietary supplementation of vitamin e (dl-alpha-tocopheryl acetate) and copper in combination with a high level of monounsaturated fatty acids were examined with regard to the antioxidant concentration and the susceptibility to lipi ... | 1999 | 10064033 |
| radiocaesium and radiostrontium uptake by turnips and broad beans via leaf and root absorption. | one of the immediate consequences of massive radioisotope release into the atmosphere is contamination of the biosphere. this contamination can affect plants either by direct deposition onto the leaves, or by contaminating the soil followed by absorption by the roots. knowledge of the efficacy of the two routes of radionuclide incorporation into the food chain is fundamental to understanding the mechanisms by which radioactive contamination reaches man. the present work analyzes the incorporatio ... | 1999 | 10070709 |
| molecular characterization of catalytic-subunit cdna sequences encoding protein phosphatases 1 and 2a and study of their roles in the gibberellin-dependent osamy-c expression in rice. | to understand the molecular mechanism of gibberellin-dependent gene regulation, the effect of three phosphatase inhibitors on the germination of rice seeds and the expression of a target gene, the alpha-amylase gene, osamy-c, were measured. we found that okadaic acid, microcystin-lr, and calyculin a, which are known to specifically inhibit ser/thr phosphatases 1 and 2a, strongly inhibit the expression of the osamy-c and may be involved in the germination of rice seeds. the protein phosphatase en ... | 1999 | 10080713 |