| metabolically driven self-restoration of energy-linked functions by avocado mitochondria: general characteristics and phosphorylative aspects. | to assess the restorative capacity of isolated avocado (persea americana) fruit mitochondria, the organelles were first aged in the absence of an energy source at 25 degrees c for several hours until respiratory control and oxidative phosphorylation were greatly diminished or totally lost. energy-linked functions were then gradually restored over a period of several hours after the addition of substrate. restoration of respiratory control resulted from both an increase in state 3 and a decrease ... | 1991 | 16668096 |
| isolation and characterization of a cellulase gene family member expressed during avocado fruit ripening. | we present in this paper the structural analysis of two members of a small cellulase gene family, designated cel1 and cel2, from avocado. these genes were isolated by screening a lambda embl3 genomic library with a ripening-induced cellulase cdna. restriction endonuclease and southern blot analyses showed that the cel1 gene is highly homologous to the cellulase cdna and thus represents a ripening-related cellulase gene. the other cellulase gene, cel2, is closely related to cel1, but is divergent ... | 1990 | 2259345 |
| n-demethylation of p-chloro-n-methylaniline catalyzed by subcellular fractions from the avocado pear (persea americana). | subcellular fractions from the avocado pear ( persea americana) catalyzed formation of p-chloroaniline from p-chloro-n-methylaniline. fractions prepared by centrifugation of avocado homogenates at 20, 000g for 20 min formed p-chloroaniline (2900 +/- 500 pmol min-1 mg protein-1) with an nadph-generating system. p-chloroaniline formation required reduced pyridine nucleotide (nadph was 6-7 times more effective than nadh) and o2. n-demethylation was inhibited by co (55% inhibition at co:o2 = 1) and ... | 1984 | 6732241 |
| interrelationship of gene expression, polysome prevalence, and respiration during ripening of ethylene and/or cyanide-treated avocado fruit. | upon initiation of ripening in avocado fruit (persea americana mill. cv hass) with 10 microliters/liter ethylene, polysome prevalence and associated poly(a)(+) mrna increase approximately 3-fold early in the respiratory climacteric and drop off to preclimacteric levels at the peak of the respiratory climacteric. the increase in poly(a)(+) mrna on polysomes early in the respiratory climacteric constitutes a generic increase in constitutive mrnas. new gene expression associated with ripening is mi ... | 1984 | 16663414 |
| avocado cellulase: nucleotide sequence of a putative full-length cdna clone and evidence for a small gene family. | a cdna library was prepared from ripe avocado fruit (persea americana mill. cv. hass) and screened for clones hybridizing to a 600 bp cdna clone (pav5) coding for avocado fruit cellulase. this screening led to the isolation of a clone (pav363) containing a 2021 nucleotide transcribed sequence and an approximately 150 nucleotide poly(a) tail. hybridization of pav363 to a northern blot shows that the length of the homologous message is approximately 2.2 kb. the nucleotide sequence of this putative ... | 1987 | 24276968 |
| regulation of climacteric respiration in ripening avocado fruit. | ripening of avocado fruit is associated with a dramatic increase in respiration. in vivo(31)p nuclear magnetic resonance spectroscopy revealed large increases in atp levels accompanying the increase in respiration. both glycolytic enzymes, phosphofructokinase, and pyrophosphate: fructose-6-phosphate phosphotransferase were present in avocado fruit with the latter activity being highly stimulated by fructose 2,6-bisphosphate. fructose 2,6-bisphosphate levels increased approximately 90% at the ons ... | 1987 | 16665374 |
| interactions of avocado (persea americana) cytochrome p-450 with monoterpenoids. | the microsomal fraction of avocado (persea americana) mesocarp is a rich source of cytochrome p-450 active in the demethylation of xenobiotics. cytochrome p-450 from this tissue has been purified and well characterized at the molecular level (dp o'keefe, kj leto [1989] plant physiol 89: 1141-1149; kr bozak, h yu, r sirevag, re christoffersen [1990] proc natl acad sci usa 87: 3904-3908). despite this extensive characterization, the role of the enzyme in vivo was not established. optical and elect ... | 1992 | 16668790 |
| characterization and kinetic parameters of ethylene-forming enzyme from avocado fruit. | biosynthesis of the phytohormone ethylene in higher plants proceeds via the following pathway: s-adenosylmethionine----1-aminocyclopropane-1-carboxylic acid (acc)----ethylene. ethylene-forming enzyme (efe), the enzyme responsible for the oxidation of acc to ethylene, has been only partially characterized in vitro. we have obtained authentic efe activity in vitro from extracts of avocado fruit (persea americana mill. cv hass). ammonium sulfate fractionation revealed the presence of two efe activi ... | 1992 | 1556109 |
| ripening-related gene from avocado fruit : ethylene-inducible expression of the mrna and polypeptide. | fruit ripening involves a series of changes in gene expression regulated by the phytohormone ethylene. avoe3, a ripening-related gene in avocado fruit (persea americana mill. cv hass), was characterized with regard to its ethylene-regulated expression. the avoe3 mrna and immunopositive protein were induced in mature fruit within 12 hours of propylene treatment. the avoe3 mrna levels reached a maximum 1 to 2 days before the ethylene climacteric, whereas the immunopositive protein continued to acc ... | 1992 | 16668676 |
| cellulase occurs in multiple active forms in ripe avocado fruit mesocarp. | the existence of multiple forms of avocado (persea americana mill. cv hass) cellulase in crude protein extracts of ripe avocado fruit is reported. cellulase was separated into at least 11 multiple forms by native isoelectric focusing in the ph range between 4 and 7 and visualized by both activity staining using congo red and immunostaining. the enzyme components were acidic proteins with isoelectric points in the range of ph 5.10 to 6.80, the predominant forms having isoelectric points of 5.60, ... | 1992 | 16668672 |
| intermediates in the recycling of 5-methylthioribose to methionine in fruits. | the recycling of 5-methylthioribose (mtr) to methionine in avocado (persea americana mill, cv hass) and tomato (lycopersicum esculentum mill, cv unknown) was examined. [(14)ch(3)]mtr was not metabolized in cell free extract from avocado fruit. either [(14)ch(3)]mtr plus atp or [(14)ch(3)]5-methylthioribose-1-phosphate (mtr-1-p) alone, however, were metabolized to two new products by these extracts. mtr kinase activity has previously been detected in these fruit extracts. these data indicate that ... | 1983 | 16663204 |
| malate oxidation and cyanide-insensitive respiration in avocado mitochondria during the climacteric cycle. | after preparation on self-generated percoll gradients, avocado (persea americana mill, var. fuerte and hass) mitochondria retain a high proportion of cyanide-insensitive respiration, especially with alpha-ketoglutarate and malate as substrates. whereas alpha-ketoglutarate oxidation remains unchanged, the rate of malate oxidation increases as ripening advances through the climacteric. an enhancement of mitochondrial malic enzyme activity, measured by the accumulation of pyruvate, closely parallel ... | 1982 | 16662684 |
| reversible effects of moderately elevated temperature on the distribution of excitation energy between the two photosystems of photosynthesis in intact avocado leaves. | initial (fo), maximum (fm) and steady-state (fs) levels of modulated chlorophyll fluorescence were measured in intact avocado leaves (persea americana mill.) during state 1-state 2 transitions using a combination of modulated and non-modulated lights with synchronized detection. under normal temperature conditions (20°c), transition from state 2 to state 1 was associated with a substantial increase (about 20%) in fm and fo whereas the fm/fo ratio remained constant, reflecting increased absorptio ... | 1987 | 24430668 |
| synthesis and processing of cellulase from ripening avocado fruit. | the biosynthesis and processing of cellulase from ripening avocado fruit was studied. the mature protein is a glycoprotein, as judged by concanavalin a binding, with a molecular weight of 54,200. upon complete deglycosylation by treatment with trifluoromethane sulfonic acid the mature protein has a molecular weight of 52,800 whereas the immunoprecipitated in vitro translation product has a molecular weight of 54,000. this result indicates that cellulase is synthesized as a large molecular weight ... | 1986 | 16664911 |
| preparation of avocado mitochondria using self-generated percoll density gradients and changes in buoyant density during ripening. | mitochondria from avocado (persea americana mill, var. fuerte and hass) can be rapidly prepared at every stage of ripening using differential centrifugation and self-generated percoll gradients. the procedure results in improved oxidative and phosphorylative properties, especially for mitochondria isolated from preclimacteric fruits.a gradual change in the buoyant density of avocado mitochondria takes place during ripening. climacteric and postclimacteric avocado mitochondria have the same buoya ... | 1982 | 16662683 |
| gene expression during fruit ripening in avocado. | the poly(a) (+)rna populations from avocado fruit (persea americana mill cv. hass) at four stages of ripening were isolated by two cycles of oligo-dt-cellulose chromatography and examined by invitro translation, using the rabbit reticulocyte lysate system, followed by two-dimensional gel electrophoresis (isoelectric focusing followed by sodium dodecyl sulfate polyacrylamide gel electrophoresis) of the resulting translation products. three mrnas increased dramatically with the climacteric rise in ... | 1982 | 24271626 |
| regulation of ethylene biosynthesis in avocado fruit during ripening. | preclimacteric avocado (persea americana mill.) fruits produced very little ethylene and had only a trace amount of l-aminocyclopropane-1-carboxylic acid (acc) and a very low activity of acc synthase. in contrast, a significant amount of l-(malonylamino)cyclopropane-1-carboxylic acid (macc) was detected during the preclimacteric stage. in harvested fruits, both acc synthase activity and the level of acc increased markedly during the climacteric rise reaching a peak shortly before the climacteric ... | 1986 | 16664762 |
| duplicated phosphoglucose isomerase genes in avocado. | avocado (persea americana) cultivars were assayed for phosphoglucose isomerase (pgi) isozymes using starch gel electrophoresis. three pgi genes were identified: one monomorphic locus, pgi-i, coding for the plastid isozyme and two independently assorting loci, pgi-2 and pgi-3, coding for the cytosolic isozymes. the genetic analysis was based on comparisons of pgi zymograms from somatic and pollen tissue and on mendelian analysis of progeny from selfed trees. the isozymic variability for pgi can b ... | 1985 | 24247459 |
| [composition of the essential oil of persea americana]. | | 1981 | 17401983 |
| sequence analysis and comparison of avocado fruit and bean abscission cellulases. | a 1700 nucleotide cdna clone for a bean (phaseolus vulgaris cv red kidney) abscission cellulase (endo-(1,4)-beta-d-glucanase) has been identified and sequenced. this cdna clone contains a 1485 nucleotide open reading frame which includes coding sequences for a putative signal peptide and mature protein. the nucleotide and deduced amino acid sequences for the bean abscission cellulase are compared to the previously reported sequences of an avocado fruit ripening cellulase. optimal alignment of th ... | 1991 | 16668074 |
| the effect of ethylene and propylene pulses on respiration, ripening advancement, ethylene-forming enzyme, and 1-aminocyclopropane-1-carboxylic acid synthase activity in avocado fruit. | when early-season avocado fruit (persea americana mill. cv hass) were treated with ethylene or propylene for 24 hours immediately on picking, the time to the onset of the respiratory climacteric, i.e. the lag period, remained unchanged compared with that in untreated fruit. when fruit were pulsed 24 hours after picking, on the other hand, the lag period was shortened. in both cases, however, a 24 hour ethylene or propylene pulse induced a transient increase in respiration, called the pulse-peak, ... | 1991 | 16668073 |
| effects of low temperature and respiratory inhibitors on calcium flux in plant mitochondria. | the effects of low temperature on uptake and release of (45)ca(2+) were studied with sound, well-coupled mitochondria extracted at room temperature from avocado (persea americana mill, cv fuerte) fruits. low ca(2+) concentrations (10 micromolar) were employed to simulate physiological conditions. at 25 degrees c, the rate of ca(2+) uptake decreased with time, whereas at 5 degrees c the initial rate, though lower, remained linear. as a consequence total uptake at 5 degrees c was substantially gre ... | 1985 | 16664154 |
| cytokinin and inhibitor activities in the avocado fruit mesocarp. | no cytokinin activity was found in methanolic mesocarp extracts after purification with petroleum ether and ethyl acetate. cytokinin activity did appear, however, in aqueous fraction after acid hydrolysis or passage through dowex 50 (h(+)) ion exchange columns. the level of this activity, the fruit growth rate, and the cell division rate were found to be positively correlated (with each other).an inhibitor to cytokinin activity in soybean callus bioassay was found in the petroleum ether fraction ... | 1970 | 16657460 |
| cytokinin activity in avocado seeds during fruit development. | the soybean callus bioassay was used to determine levels of cytokinin activity in avocado (persea americana) seeds.in the embryo, levels are high during the early stages of development, but diminish as the fruit grows. the level of cytokinin activity in the endosperm is very high throughout the period that this tissue exists. the seed coats have very high activity levels while the fruit is young, reaching values comparable with those found in the endosperm. the activity level falls as the rate o ... | 1970 | 16657459 |
| the biosynthesis of steryl glucosides in plants. | mitochondrial preparations from pea root (pisum sativum l. var. alaska) cauliflower inflorescence (brassica cauliflora gars.) and avocado inner mesocarp (persea americana mill. var. fuerte), and chloroplast preparations from spinach leaf (spinacia oleracea l. var. bloomsdale) incorporate glucose into steryl glucoside and acylated steryl glucoside when either uridine diphosphate-glucose or uridine diphosphate-galactose is supplied as precursor. in the case of pea root mitochondria, galactosyl dig ... | 1970 | 5423466 |
| comparative studies of effect of auxin and ethylene on permeability and synthesis of rna and protein. | the effects of ethylene on permeability and rna and protein synthesis were assayed over a 6 to 26 hr period in tissue sections from avocado (persea gratissima gaertn. f., var. fuerte), both pulp and peel of banana (musa sapientum l., var. gros michel), bean endocarp (phaseolus vulgaris l., var. kentucky wonder pole beans) and leaves of rhoeo discolor. ethylene had no effect on permeability in 4 of the 5 tissues, but sometimes enhanced solute uptake in banana peel; it had either no effect or an i ... | 1969 | 16657212 |
| intratumor chemotherapy in combination with a systemic antimetastatic drug in the treatment of lewis-lung carcinoma. | the effect of an antimetastatic agent plus intratumor chemotherapy was evaluated in mice bearing lewis-lung carcinoma by measuring survival time and by histological examination. polymeric flavan-3,4-diol (apf) from avocado seeds, persea gratissima, administered alone directly into the tumor did not change survival time, although it partially destroyed the primary tumor. however, the drug administered in combination with an antimetastatic, 1,2-bis(3,5-dioxopiperazin-1-yl)ethane (icrf-154), result ... | 1985 | 3835980 |
| cellulase gene expression in ripening avocado fruit: the accumulation of cellulase mrna and protein as demonstrated by cdna hybridization and immunodetection. | a cdna library was constructed from poly(a)(+)rna of ripe avocado fruit. colony hybridization identified a number of ripening specific clones of which one, pav5, was shown to be specific for cellulase. hybrid selection with pav5 provided a message from ripe fruit that on in vitro translation yielded a polypeptide of 53kd, comigrating with purified avocado cellulase on sds polyacrylamide gel electrophoresis. the translation product was selectively immunoprecipitated by antiserum to purified avoca ... | 1984 | 24310572 |
| partial purification and characterization of arginine decarboxylase from avocado fruit, a thermostable enzyme. | a partially purified preparation of arginine decarboxylase (ec 4.1.1.19), a key enzyme in polyamine metabolism in plants, was isolated from avocado (persea americana mill. cv fuerte) fruit. the preparation obtained from the crude extract after ammonium sulfate precipitation, dialysis, and heat treatment, had maximal activity between ph 8.0 and 9.0 at 60 degrees c, in the presence of 1.2 millimolar mncl(2), 2 millimolar dithiothreitol, and 0.06 millimolar pyridoxal phosphate. the k(m), of arginin ... | 1984 | 16663805 |
| postharvest variation in cellulase, polygalacturonase, and pectinmethylesterase in avocado (persea americana mill, cv. fuerte) fruits in relation to respiration and ethylene production. | cellulase, polygalacturonase (pg), pectinmethylesterase (pme), respiration, and ethylene production were determined in single "fuerte" avocado fruits from the day of harvest through the start of fruit breakdown. pme declined from its maximum value at the time of picking to a low level early in the climacteric. pg activity was not detectable in the preclimacteric stage, increased during the climacteric, and continued to increase during the postclimacteric phase to a level three times greater than ... | 1979 | 16660954 |
| respiratory contribution of the alternate path during various stages of ripening in avocado and banana fruits. | the respiration of fresh slices of preclimacteric avocado (persea americana mill. var. hass) and banana (musa cavendishii var. valery) fruits is stimulated by cyanide and antimycin. the respiration is sensitive to m-chlorobenzhydroxamic acid in the presence of cyanide but much less so in the presence of antimycin. in the absence of cyanide the contribution of the cyanide-resistant pathway to the coupled preclimacteric respiration is zero. in uncoupled slices, by contrast, the alternate path is e ... | 1978 | 16660495 |
| the effect of indole-3-acetic acid and other growth regulators on the ripening of avocado fruits. | observations were made of the effects of several plant regulators, indole-3-acetic acid, kinetin, abscisic acid, and gibberellic acid, as well as of extracts prepared from leaves and fruit stalks on the respiration pattern, ethylene production, and the number of days to ripen of avocado fruits (persea americana mill.). these substances were vacuum infiltrated to insure good penetration and distribution. kinetin, abscisic acid, gibberellic acid, and the extracts had no effect on either ripening t ... | 1975 | 16659195 |
| acid phosphatase development during ripening of avocado. | the activity and subcellular distribution of acid phosphatase were assayed during ethylene-induced ripening of whole fruit or thick slices of avocado (persea americana mill. var. fuerte and hass). the activity increased up to 30-fold during ripening in both the supernatant fraction and the triton x-100 extract of the precipitate of a 30,000g centrifugation of tissue homogenates from whole fruit or slices ripening in moist air. enzyme activity in the residual precipitate after triton extraction r ... | 1975 | 16659087 |
| the synthesis of fatty acids in avocado mesocarp and cauliflower bud tissue. | 1. plastid and mitochondrial preparations were obtained by density-gradient centrifugation of homogenates made by gentle disintergration of avocado fruit mesocarp and cauliflower bud tissue. 2. the mitochondrial preparations had respiratory activity but did not incorporate [1-14c]acetate into fatty acids. 3. the plastid preparations incorporated [1--14c]acetate into the range of fatty acids found in the parent tissue. no fatty acid synthetase activity could be detected in the 12000g supernatant ... | 1975 | 1156380 |
| effects of preincubation invitro with 3, 4-benzopyrene and phenobarbital on the drug-metabolism systems present in the microsomal and soluble fractions of the avocado pear (persea americana). | | 1975 | 1132559 |
| cellulase activity and fruit softening in avocado. | cellulase activity in detached avocado (persea americana mill.) fruits was found to be directly correlated with ripening processes such as climacteric rise of respiration, ethylene evolutin, and softening. this activity in the pericarp could be induced by ethylene treatment, and the more mature the fruit-the faster and the greater was the response. only a very low cellulase activity could be detected in hard avocado fruit right after harvest. cellulase activity was highest at the distal end of t ... | 1978 | 16660305 |
| the maintenance of respiratory control at 25 degrees c by mitochondria from various animal and plant sources. | 1. mitochondria from four different animal and five different plant sources exhibit a wide variation in their capacity to maintain respiratory control at 25 degrees c. 2. beef heart mitochondria, and pear and avocado fruit mitochondria exhibit the longest retention of respiratory control extending to periods of approximately 80 and 40 hr, respectively. | 1978 | 318361 |
| succinoxidase activity of avocado fruit mitochondria in relation to temperature and chilling injury throughout the climacteric cycle. | mitochondria were isolated from ;fuerte' avocado fruit (persea americana mill.) at four different stages of the respiratory climacteric. preclimacteric fruit had the highest rate of succinate oxidation and the postclimacteric mitochondria the lowest. subsequently, successive additions of adp increased the respiratory control ratio.arrhenius plots of succinate oxidation of intact mitochondria from climacteric rise and climacteric peak fruit showed two transition temperatures, while only one was o ... | 1977 | 16660117 |
| ripening and in vitro retention of respiratory control by avocado and pear mitochondria. | the retention of respiratory control ("survival") by mitochondria held at 25 c was studied in relation to the ripening of two varieties of avocado (persea americana mill. var. ;fuerte' and ;hass') and one variety of pear (pyrus communis. l. var. ;bartlett') fruit. the survival of avocado mitochondria increased from 8 to 10 hours when isolated from unripe, preclimacteric fruit, to 48 hours when isolated from fully ripe, postclimacteric fruits. although rates of alpha-ketoglutarate oxidation, resp ... | 1975 | 16659279 |
| respiration & internal atmosphere of avocado fruit. | | 1963 | 16655774 |
| metabolic processes in cytoplasmic particles of the avocado fruit. iii. the operation of the tricarboxylic acid cycle. | | 1957 | 16654950 |
| relation of the production of an active emanation to respiration in the avocado fruit. | | 1944 | 16653933 |
| growth and water relations of the avocado fruit. | | 1936 | 16653349 |
| totipotency of cells from fruit pericarp tissue in vitro. | callus derived from avocado fruit pericarp grown in vitro for several generations developed roots with stele, endodermis, cortex, epidermis, and root cap. no correlation between environment and root production was demonstrated. | 1962 | 17832008 |
| metabolic processes in cytoplasmic particles of the avocado fruit. iv. ripening and the supernatant fraction. | | 1957 | 16655063 |
| metabolic processes in cytoplasmic particles of the avocado fruit. v. effect of oxalacetate on the oxidation of pyruvate and succinate. | | 1957 | 13416272 |
| metabolic processes in cytoplasmic particles of the avocado fruit. vii. oxidative and phosphorylative activities throughout the climacteric cycle. | | 1965 | 5842698 |
| effect of ionizing radiation on respiration and ethylene production of avocado fruit. | | 1965 | 5833212 |
| metabolic processes in cytoplasmic particles of the avocado fruit. vi. controlled oxidations and coupled phosphorylations. | | 1964 | 16655919 |
| [detection of vitamin a in the oil of the avocado persea gratissima gaerth]. | | 1963 | 14092099 |
| the effect of tonicity and metabolic inhibitors on respiration and ripening of avocado fruit slices. | the ripening of avocado (persea americana mill.) fruit slices was inhibited whether they were floated in water or in buffered aqueous 0.3 m mannitol, 0.25 m kcl, and sucrose. there was no evidence to support the contention that ripening occurred when the tonicity of the bathing medium was increased. decreased gaseous exchange is considered to be a major cause of this inhibition because by utilizing a technique that afforded better aeration, slices could be water infiltrated and still ripen norma ... | 1974 | 16658998 |
| similarities between the actions of ethylene and cyanide in initiating the climacteric and ripening of avocados. | a continuous exposure of intact avocados (persea americana) to 400 mul/l of cyanide results in a rapid increase in the rate of respiration, followed by a rise in ethylene production, and eventual ripening. the pattern of changes in the glycolytic intermediates glucose 6-phosphate, fructose diphosphate, 3-phosphoglyceric acid, and phosphoenolpyruvate during the rapid rise in respiration in both ethylene and cyanide-treated fruits is similar to that found in fruits made anaerobic where a 2.3- to 3 ... | 1974 | 16658918 |
| [isolation and determination of vitamin d in avocado (persea gratissima)]. | | 1974 | 4447702 |
| postharvest response of avocado fruits of different maturity to delayed ethylene treatments. | the effect of 10, 100, 1000, 10,000 ppm of ethylene, applied for 3, 6, 12, and 24 hours, on the ripening rate of "hass" avocado (persea americana mill.) fruits at three stages of maturity was investigated. ethylene treatments were started either immediately after picking or 2 days later. a sharp peak of ethylene production was found to precede full softening by about 2 days and the occurrence of this peak was used to determine ripening rate. hastening of fruit ripening was much more marked follo ... | 1974 | 16658812 |
| water-deficit stress, ethylene production, and ripening in avocado fruits. | differential rates of water loss were achieved in picked avocado (persea americana mill.) fruits, either by controlling the evaporation rate or by supplying water through the fruit stalk. a negative linear correlation was found between the daily rate of water loss from fruits and their ripening, as determined by the time from harvest to peak of ethylene production. ripening rate was hastened by 40% in fruits which had lost water at rate of 2.9% fresh weight per day, compared with those which los ... | 1974 | 16658649 |
| metabolic processes in cytoplasmic particles of the avocado fruit. 8. particles from different regions of the fruit. | | 1966 | 5916345 |
| protein synthesis in avocado fruit tissue. | | 1966 | 5966519 |
| metabolism of carbon-14-labeled benzene and tolueue in avocado fruit. | | 1969 | 16657134 |
| phosphorylation in avocado fruit slices in relation to the respiratory climacteric. | the rate of uptake of inorganic phosphate by tissue discs from both preclimacteric and climacteric peak avocados is linear for at least 60 minutes. the loss of (32)p upon excessive washing was much greater from peak than from preclimacteric tissue. short incubation periods and, most important, rapid washing procedures are essential for meaningful comparisons.phosphate esterification proceeded at a much greater rate in climacteric than in preclimacteric tissue. the phosphorylation was sensitive t ... | 1967 | 16656663 |
| metabolic processes in cytoplasmic particles of the avocado fruit. ix. the oxidation of pyruvate and malate during the climacteric cycle. | mitochondria isolated from preclimacteric avocado fruit oxidize pyruvate at a much lower rate than those separated from climacteric fruit. the external addition of thiamine pyrophosphate (tpp) increased the rate of pyruvate oxidation in both cases. the study of the influence of tpp on the rate of oxidation of malate by mitochondria obtained from both preclimacteric and climacteric fruit indicated that the effect of this cofactor could be understood by assuming that malate was converted to pyruva ... | 1967 | 6042356 |
| protein and nucleic acid metabolism in fruits. i. studies of amino acid incorporation during the climacteric rise in respiration of the avocado. | incorporation of (14)c l-valine and (14)c l-leucine into protein of tissue slices of the avocado fruit was relatively high during the early stages of the climacteric rise, declined sharply thereafter, and was virtually absent at the peak. the incorporation of amino acids in the preclimacteric stage was markedly lower than during the early stage of the respiratory rise. by following incorporation in relation to uptake at several concentrations it was established that the results were not a reflec ... | 1966 | 5978544 |
| some aspects of carnitine metabolism in avocado (persea americana) (short communication). | the fact that palmitoyl-l-carnitine is oxidized by avocado mitochondria at a rate comparable with that of succinate oxidation suggests that there are at least two systems for beta-oxidation in higher plants. the carnitine-associated system is located in a mitochondrial fraction, whereas the glyoxylate-cycle-associated system is located in the glyoxysomes. | 1973 | 16742829 |
| lipid composition of plant mitochondria and of chloroplasts. | the mitochondrial lipids from avocado fruit, cauliflower buds, and potato tubers, and the lipids of chloroplasts isolated from avocado fruit and from cauliflower leaves were identified and the concentrations were determined. the lipid composition was compared with that of beef heart mitochondria. phospholipids constituted 50-56% of total lipids in plant mitochondria while this fraction made up 90% of the lipids in beef heart mitochondria. in both cases the chief phospholipids were phosphatidylch ... | 1973 | 4698270 |
| [study of the nutritional value of avocado--persea americana miller]. | | 2009 | 4659314 |
| stereochemical aspects of the formation of double bonds in abscisic acid. | the stereochemistry of the hydrogen elimination that occurs during the formation of the delta(4)- and delta(2)'-double bonds of abscisic acid has been determined from the (14)c/(3)h ratios in abscisic acid biosynthesized by avocado fruit from [2-(14)c,(2r)-2-(3)h(1)]-, [2-(14)c,(2s)-2-(3)h(1)]- and [2-(14)c,(5s)-5-(3)h(1)]-mevalonate. setting the (14)c/(3)h ratio at 3:3 for [2-(14)c,(2r)-2-(3)h(1)]mevalonate, the corresponding ratio in derived methyl abscisate was 3:2.28; the analogous ratio for ... | 1972 | 4643698 |
| pectin methylesterase and polygalacturonase in avocado fruit at various stages of development. | | 1972 | 16658064 |
| the site of fatty acid biosynthesis in the avocado pear. | | 1970 | 5492822 |