| microbial growth and production of antibiotics. | | 1978 | 357271 |
| mitogenic effects of bacterial cell walls, their fragments, and related synthetic compounds on thymocytes and splenocytes of guinea pigs. | stimulation of [(3)h]thymidine incorporation of thymocytes and splenocytes from guinea pigs by various bacterial cell walls and their peptidoglycans, by enzymatic digests, and by synthetic muramyl dipeptides was studied as an indication of mitogenic activity. cell wall and peptidoglycan preparations, isolated from 19 strains belonging to 18 different species, definitely increased [(3)h]thymidine incorporation of thymocytes as well as splenocytes, regardless of mycolic acid contents as a non-pept ... | 1979 | 314932 |
| erythrocyte surface: novel determinant of drug susceptibility in rodent malaria. | to study the role of the erythrocyte membrane in the process of chloroquine accumulation, surface polypeptides were digested with a nonspecific protease from streptomyces griseus. this treatment activated a saturable process of chloroquine accumulation with an affinity and a specificity similar to those of mouse erythrocytes infected with plasmodium berghei cs (chloroquine susceptible). studies of competitive inhibitors of chloroquine accumulation yielded the following approximate values for k(i ... | 1978 | 358916 |
| effects of experimental conditions on the interaction of filipin and pimaricin with cholesterol. | | 1979 | 316813 |
| formation, properties, and germination of actinomycete spores. | | 1978 | 360964 |
| extrachromosomally determined antibiotic production. | | 1978 | 360970 |
| a method of rapid wetting and synchronous germination of streptomycete spores. | | 1977 | 321309 |
| ssti: a restriction endonuclease from streptomyces sp. stanford. | a strain of streptomyces has been isolated which is a convenient source of a new restriction endonuclease. the enzyme has been prepared from extracts of these cells and its cleavage sites localized on phage lambda dna. the enzyme, termed ssti, produces cohesive ends and should be useful for molecular cloning experiments. | 1978 | 365688 |
| streptomycin: irreversible association with superoxide dismutases. | | 1978 | 367274 |
| lambdaplac5 derivatives, potential vectors for dna fragments cleaved by streptomyces stanfordii restriction enzyme (ssti). | | 1977 | 338420 |
| anthracyclines. | | 1977 | 321310 |
| production of polyene macrolide antibiotics. | | 1977 | 322452 |
| [mutagenic effect of new chemical compounds. iv. mutagenic effect of dialkylaminoethyl esters of 5,6-dihydro-7h-benz(c)carbazol-carboxylic acids]. | the mutagenic effect of dialkylaminoet hyl esters of 5,6-dihydro-7h-benz(c)carbazole-carboxylic acids on biochemical mutants (escherichia coli p-678, actinomyces rimosus 222) is found. hydrochloride of diethylaminoethyl ester of 5,6-dihydro-7h-benz(c)carbazole-9-carboxylic acid, which induced reversible and direct mutations, proved to be the most active compound, its mutagenic activity exceeding considerably the activity of ethylene imine. | 1977 | 340341 |
| dna restriction site mapping of adenovirus type 16 with bami, ecori, hpai and sa/i. | | 1977 | 324798 |
| subunit structure of external invertase from saccharomyces cerevisiae. | because 50% of the mass of the external invertase of saccharomyces cerevisiae consists of carbohydrate, it has been extremely difficult to obtain an accurate molecular weight of this enzyme by centrifugal or electrophoretic techniques. however, on removing almost all of the oligosaccharide chains of this enzyme with the endo-beta-n-acetyl-glucosaminidase h from streptomyces plicatus, it has been possible to show that carbohydrate-free invertase is composed of two 60,000-dalton subunits. terminal ... | 1977 | 325007 |
| new microbial growth factor. | a screening procedure was used to isolate from soil a penicillium sp., two bacterial isolates, and a streptomyces sp. that produced a new microbial growth factor. this factor was an absolute growth requirement for three soil bacteria. the penicillium sp. and one of the bacteria requiring the factor, an arthrobacter sp., were selected for more extensive study concerning the production and characteristics of the growth factor. it did not seem to be related to the siderochromes. it was not present ... | 1977 | 327929 |
| pyrrolo(1,4)benzodiazepine antitumor antibiotics. comparative aspects of anthramycin, tomaymycin and sibiromycin. | | 1977 | 328469 |
| biosynthesis of aurodox (antibiotic x-5108). incorporation of 14c-labelled precursors into aurodox. | | 1977 | 328470 |
| spontaneous and induced variability in streptomyces nogalater producing nogalamycin. | variability in the production of nogalamycin by streptomyces nogalater var. nogalater was followed in untreated and mutagenized populations of the standard strain nrrl 3035 and its spontaneous variant k-18 using the method of agar blocks with subsequent tests under submerged conditions. in both strains the most active variants were obtained by natural selection without mutagenic treatment; in this way productivity increased by 108% after two selection steps. treatment with uv-radiation did not y ... | 1977 | 330365 |
| [biosynthesis of the nucleotide portion of the vitamin b12 molecule by microorganisms]. | | 1977 | 333243 |
| chartreusin: production and microbiological assay. | chartreusin was produced in the fermentation liquors of streptomyces chartreusis at peak concentrations of 200 to 300 mug/ml. the titers could be increased by 200 to 300% or more by incorporating d-fucose, a part of the chartreusin molecule, into the fermentation media. a microbiological assay with sarcina lutea could detect concentrations of the drug of 0.5 to 1.0 mug/ml. | 1977 | 335963 |
| antibiotics produced by streptomyces olivaceus 142. antifungal properties of antibiotic wr-142 fpg. | antifungal activity of antibiotic wr-142 fpg was evaluated in vitro and in vivo. in vitro, the minimal inhibitory concentration (mic) for candida and rhodotorula was 0.1 microgram/ml (preparation i) and 0.003 microgram/ml (preparation ii). in vivo, experiments were carried out with model of subacute candidiasis in balb mice. the criteria for antifungal activity of wr-142 fpg were based on survival time of infected animals and numbers of live c. albicans cells in the kidneys and spleen of animals ... | 1979 | 383040 |
| specific inhibition of human group i pepsins by two pepsin inhibitiors. | two pepsin-inhibitors, a peptide produced by streptomyces ef-44-201 and a peptide produced by actinomycetes have been found to inactivate completely the human group i pepsin, but to have little activity against the group ii pepsins. | 1977 | 336448 |
| classification and identification of actinomycetales causing actinomycosis. | | 1979 | 384381 |
| polyether antibiotics: versatile carboxylic acid ionophores produced by streptomyces. | | 1977 | 337768 |
| interspecies matings of streptomyces fradiae with streptomyces bikiniensis mediated by conventional and protoplast fusion techniques. | | 1978 | 356946 |
| increased production of the antibiotic aurodox (x-5108) by aurodox-resistant mutants. | conventional strain and media improvement techniques were of limited success in increasing yields of the antibiotic aurodox (x-5108) above 0.5 g/liter. higher yields were obtained by reversion of a zero producer followed by the selection of mutants resistant to aurodox. resistant strains of streptomyces goldiniensis atcc 21386 able to grow on 2 g/liter of aurodox produced greater than 2.5 g/liter of antibiotic. the rate of yield increase leveled off as the strains became resistant to greater tha ... | 1978 | 357362 |
| [antibiotics of the carbonyl-conjugated pentaene group]. | | 1978 | 363178 |
| [use of indirect immunofluorescence to study the soil actinomycete streptomyces olivocinereus]. | | 1978 | 370512 |
| carriomycin, a new polyether antibiotic produced by streptomyces hygroscopicus. | carriomycin, a new polyether antibiotic, was isolated from culture broth of streptomyces hygroscopicus strain t-42082. it is active against gram-positive bacteria, several fungi, yeasts and mycoplasma. it is also coccidiostatic. the free acid of carriomycin occurs as colorless prisms having the molecular formula c47h80o15 (m.w. 885.15), m.p. 120 approximately 122 degrees c and [alpha]25d -0.5 in methanol. it has no characteristic absorption maxima in the ultraviolet spectrum. the presence of one ... | 1978 | 342475 |
| fine structure, physiology and biochemistry of arthrospore germination in streptomyces antibioticus. | during germination, streptomyces antibioticus arthrospores passed through stages: darkening, swelling and germ tube emergence. the first stage, darkening, whose main features were a decrease in absorbance and a loss of refractility, only required exogenous divalent cations (ca2+, mg2+ or fe2+) and energy that can be obtained from the spore reserves. this stage was blocked by agents that inhibit atp formation but not by antibiotics that inhibit macromolecular synthesis. the second stage, swelling ... | 1978 | 347027 |
| globomycin, a new peptide antibiotic with spheroplast-forming activity. ii. isolation and physico-chemical and biological characterization. | the peptide antibiotic globomycin was extracted from the culture filtrate of streptomyces halstedii no. 13912, purified on silica-gel columns and crystallized from acetonitrile to give colorless needles. it is a neutral substance with m.p. of 115 degrees c and a molecular formula of c32h57n5o9. on amino-acid analysis, it gave serine, threonine, glycine and an unidentified amino acid. it is soluble in methanol, ethyl acetate and chloroform but sparingly soluble in water. the antimicrobial spectru ... | 1978 | 353012 |
| microbiological mutagenicity studies of pesticides in vitro. | 14 pesticides were tested as pure compounds for the induction of point mutations in four strains of salmonella typhimurium--ta1535, ta1536, ta1537 and ta1538--in the presence and in the absence of rat-liver microsomal fractions and for the induction of resistance to low concentrations of streptomycin in the filamentous bacterium, streptomyces coelicolor. the technique used was essentially the so-called "spot test". the pesticides investigated were: aminotriazole, benomyl, captafol, captan, dichl ... | 1978 | 353549 |
| "maturation" of dna duplexes. | reassociation of typical single-copy dnas, like e. coli dna, even when performed at relatively low temperatures, results in the formation of perfect duplexes with thermal stability very close to that of the native dna. in contrast, duplexes of mouse repeated dna as well as duplexes of streptomyces dna prepared under the same conditions, show a low thermal stability and undergo post-reassociation changes upon prolonged incubation. these changes, called "maturation" of the dna duplexes, result in ... | 1978 | 355859 |
| antibiotic nrc-501, a new antibiotic produced by streptomyces species. | a new antibiotic, nrc-501, was isolated and purified from the fermentation broth of streptomyces species nrc-501. physical, chemical, and biological characteristics of the antibiotic nrc-501 were revealed. | 1978 | 356477 |
| coupled evolution: adaptive interactions among the genomes of plasmids, viruses, and cells. | | 1978 | 385526 |
| preliminary investigation of the use of the enzyme linked immunosorbent assay (elisa) in the serodiagnosis of mycetoma. | antibody levels in a small group of sudanese patients with clinically diagnosed mycetoma, and control groups were measured by countercurrent immunoelectrophoresis (cie) and enzyme linked immunosorbent assay (elisa). antigens were prepared from the following organisms: streptomyces somaliensis, actinomadura madurae, a. pelletieri, nocardia brasiliensis and madurella mycetomi. positive reactions were obtained in clinical cases against the homologous antigens in both cie and elisa; all heterologous ... | 1978 | 360443 |
| cefoxitin: a review of its antibacterial activity, pharmacological properties and therapeutic use. | cefoxitin is a beta-lactam antibiotic derived from cephamycin c, a naturally occurring substance produced by streptomyces lactamdurans. its resistance to destruction by beta-lactamases results in a broad spectrum of antibacterial activity which includes anaerobic as well as gram-positive and gram-negative aerobic bacteria, including many resistant to cephalothin and other cephalosporins. given by intravenous or intramuscular injection, cefoxitin is effective against a wide variety of infections ... | 1979 | 369806 |
| [biosynthesis of beta-lactam antibiotics (author's transl)]. | | 1979 | 387944 |
| inhibition of translation in bacterial and eukaryotic systems by the antibiotic anthelmycin (hikizimycin). | anthelmycin inhibits protein synthesis on both pro- and eukaryotic ribosomes by preventing the peptide bond-forming reaction. the drug is structurally similar to certain other 4-aminohexosyl cytosine antibiotics including blasticidin s, gougerotin, amicetin and bamicetin although unlike these compounds anthelmycin lacks an aminoacyl moiety. it is proposed that anthelmycin inhibits the ribosomal peptidyl transferase centre by associating with a site that overlaps the (related) ribosomal receptor ... | 1979 | 371683 |
| [structure and functioning of the kanamycin transposon of actinomycetes transferred in vitro into escherichia coli k-12]. | | 1979 | 371941 |
| [persistency and resistance of streptococci isolated from periodontal pockets]. | intradermal injection of cell walls or cell wall constituents (peptidoglycane) of streptococcus sanguis ii in experimental animals caused a similarly severe inflammatory reaction as with streptococcus a. the three "viridans" species of streptococci proved to be resistant to complement (active serum) as well as to lysozyme and were superior to streptococcus a in their capacity for resistance to another type of muralytic enzyme isolated from streptomyces albus. the new acylureido penicillins (mezl ... | 1979 | 374053 |
| isolation and characterization of antibiotic x-14547a, a novel monocarboxylic acid ionophore produced by streptomyces antibioticus nrrl 8167. | a novel carboxylic acid ionophore, antibiotic x-14547a, closely related to the polyether antibiotics has been isolated along with four other metabolites from fermented cultures of a new strain of streptomyces antibioticus. the structure, determined by x-ray analysis of the r(+)-1-amino-1-(4-bromophenyl)-ethane salt contained pyrrole carbonyl and trans-butadienyl chromophores in addition to the unusual tetrahydroindane bicyclic ring system. a second novel metabolite was identified as 3-ethyl-1,3- ... | 1979 | 374323 |
| solid media containing carboxymethylcellulose to detect cx cellulose activity of micro-organisms. | solid media containing carboxymethyl cellulose (cmc) were developed to detect cx cellulose-producing micro-organisms. hydrolysis of cmc was seen as a clear zone around colonies after flooding plates with 1% aqueous hexadecyltrimethyl-ammonium bromide. tests with ten bacterial and four fungal species showed that the degree of substitution (ds) of the cmc affects both growth and enzyme production. most of the organisms produced more cx cellulase on cmc with a ds of 0-9, but cmc with a ds of 0-4 wa ... | 1977 | 401863 |
| construction and properties of hybrids obtained in interspecific crosses between streptomyces coelicolor a3(2) and streptomyces griseus kr-15. | recombinants between streptomyces coelicolor a3(2) and streptomyces griseus kr-15 were obtained using methods of hybrid construction. recombinant rcg1, obtained from a cross between s. griseus and a s. coelicolor uf (scpi-) strain, phenotypically resembled s. coelicolor uf strains and in crosses with a s. coelicolor nf donor strin produced recombinatn progeny at a frequency of 100%. recominant rcg3, like scp1-carrying s. coelicolor strains, inhibited scp1-strains of s. coelicolor and in crosses ... | 1977 | 401864 |
| antibiotic glycosides. 8. erythromycin d, a new macrolide antibiotic. | | 1977 | 402407 |
| [toxicity of bacterial polysaccharides (a review of the literature)]. | | 1977 | 402887 |
| x-14547a, a new ionophorous antibiotic produced by streptomyces antibioticus nrrl 8167. discovery, fermentation, biological properties and taxonomy of the producing culture. | x-14547a is a novel antibiotic produced by a new strain of streptomyces antibioticus (nrrl 8167). the antibiotic is active in vitro against gram-positive bacteria and is capable of complexing and transporting divalent as well as monovalent metal cations. | 1979 | 374324 |
| ultrastructural investigations on surface structures involved in coxiella burnetii phase variation. | by using the cytochemical staining procedure with concanavalin a, horseradish peroxidase, and diaminobenzidine, no surface carbohydrates with terminal alpha-glucosyl or sterically closely related residues could be detected on the cell walls of coxiella burnetii phases i and ii. using a polycationized ferritin derivative as a cytochemical probe, anionic binding sites were visualized in the electron microscope on cell membranes of c. burnetii phase ii, but not on phase i organisms. the sites appea ... | 1977 | 404251 |
| pulvomycin, an inhibitor of prokaryotic protein biosynthesis. | antibiotic 1063-z isolated from culture fluids of streptoverticillium mobaraense was identified as pulvomycin. pulvomycin was observed to inhibit protein biosynthesis in growing cells of bacillus brevis. the poly(u)-directed poly(phe) synthesis in cell-free systems of bacillus brevis and escherichia coli was highly susceptible to the antibiotic. pulvomycin did not affect the transfer of phe to trna. the results suggest that the target of pulvomycin action is the polypeptide chain elongation. | 1979 | 375861 |
| effect of penicillin on streptomycin production by streptomyces griseus. | the correlation between the biosynthesis of the cell wall and the formation of streptomycin (sm) in streptomyces griseus, influenced by a specific inhibitor of cell wall synthesis, was investigated. penicillin, in subinhibitory concentrations (1 to 5 mug/ml), was added to cultures of s. griseus in different stages of its life cycle. the inhibitor decreased sm production, when young cultures were treated; however, there was an increase in sm formation when penicillin was added to older cultures. ... | 1977 | 404959 |
| microbial formation of 4-thiouracil. | a soil organism identified as streptomyces libani var. soldani was found to produce 4-thiouracil. the product was isolated in a yield of 150 mug/ml of filtered beer and characterized by c-13 magnetic resonance and high-resolution mass spectroscopy. the product has a broad antibacterial spectrum but low specific activity. | 1977 | 404965 |
| feedback inhibition of the synthesis of an antibiotic: aurodox (x-5108). | the effect of aurodox on its own biosynthesis by streptomyces goldiniensis was studied. it was found that addition of exogenous aurodox inhibits further accumulation of aurodox by the antibiotic-producing culture. both long term fermentation studies with aurodox-14c and precursor incorporation studies over short time periods indicated that aurodox synthesis was regulated by feedback inhibition. the concentration of aurodox required to completely block further synthesis of the antibiotic was abou ... | 1977 | 405357 |
| primary structure of a chloramphenicol acetyltransferase specified by r plasmids. | naturally occurring isolates of chloramphenicol-resistant bacteria commonly synthesise chloramphenicol acetyltransferase (ec 2.3.28; cat) in amounts which are sufficient to account for the resistance phenotype and often harbour plasmids which carry the structural gene for cat. the findings of cat in such diverse prokaryotes as proteus mirabilis, agrobacterium tumefaciens, streptomyces sp., and a soil flavobacterium has led to speculation concerning the origin and evolution of the more commonly o ... | 1979 | 390404 |
| practical aspects of selection of streptomyces mutants. | the methods of industrial strains selection on the basis of some regulatory mechanisms are presented. the selection of the producing s. erythreus mutants exhibiting higher activity for transformation of erythromycin c to erythromycin a is one of the examples for the practical use of the presented method. some new techniques including isotopic methods are presented. | 1979 | 383039 |
| microbiological transformations of delta6a10a-tetrahydrocannabinol. | a screening program was conducted to find microorganisms that catalyze transformation reactions with cannabinoids. three hundred fifty-eight cultures, consisting of 97 bacteria, 175 actinomycetes, and 86 molds, were incubated in media containing 0.5 mg of delta(6a,10a)-tetrahydrocannabinol (delta(6a,10a)-thc) per ml. after 120 h of cultivation, ethyl acetate extracts of the cultures were examined by thin-layer chromatography (tlc) for transformation products. about 18% of the cultures modified d ... | 1977 | 406841 |
| method for the rapid screening of cellulolytic streptomycetes and their mutants. | a method for the rapid screening of cellulolytic streptomycetes and their mutants is reported. the technique consists of a plate assay on media containing filter paper fibres as cellulose substrate. the cellulolytic activity is detected and measured by the formation of clearing zones around the streptomycete colonies. the sensitivity of the method is increased considerably by subjecting the plates to an additional incubation period at 43 degrees c in the presence of a buffer at ph 5.3. by replic ... | 1979 | 383252 |
| glycinothricin, a new streptothricin-class antibiotic from streptomyces griseus. | glycinothricin is a streptothricin-class antibiotic obtained for the first time from the culture broth of a strain of streptomyces griseus. glycinothricin, the deformimino derivative of antibiotic ll-ab664, gives n-methylstreptolidine, n-methyl-d-glucosamine and glycine on acid hydrolysis. in comparison with ll-ab664, glycinothricin is less active against gram-positive and gram-negative bacteria and less toxic to mice. | 1977 | 407205 |
| peptidyl transfer ribonucleic acid hydrolase activity of proteinase k. | proteinase k, a seryl-protease obtained from tritirachium album, is able to specifically hydrolyze n-blocked aminoacyl transfer ribonucleic acids (trnas). the blocked amino acid is released, and the trna molecule remains able to be recharged by its cogante amino acid. aminoacyl-trnas are highly resistant to hydrolysis by the protease. this activity is not due to contamination of the protease preparation. a commercial protease from streptomyces griseus displayed a similar activity, while trypsin, ... | 1979 | 385046 |
| immobilization of enzymes and microbial cells using carrageenan as matrix. | conditions for the gelation k-carrageenan, which is a new polymer for immobilization of enzymes and microbial cells, were investigated in detail. k-carrageenan was easily induced to gel by contact with metal ions, amines, amino acid derivatives, and water-miscible organic solvents. by using this property of k-carrageenan, the immobilization of enzymes and microbial cells was investigated. several kinds of enzymes and microbial cells were easily immobilized with high enzyme activities. immobilize ... | 1979 | 385076 |
| [actinomycetales in cutaneous pathology]. | after discussing the classification of the actinomycetales, the authors give a detailed description of the epidemiology and particularly of the clinical features of cutaneous diseases induced by genera such as actinomyces, norcardia and streptomyces. histopathological and immunological studies, together with laboratory and differential diagnostics, are also examined. in the last section, present therapeutic treatments are briefly outlined. | 1979 | 385380 |
| epoxidation of aldrin to exo-dieldrin by soil bacteria. | twenty-two strains of soil bacteria, including representatives of the genera bacillus, micromonospora, mycobacterium, nocardia, streptomyces, thermoactinomyces, and pseudomonas and 10 unidentified gram-negative, motile, rod-shaped bacteria, were shown to degrade aldrin to its epoxide dieldrin. in every case, the exo-stereoisomer of dieldrin was produced exclusively. | 1977 | 407844 |
| progress in space biology. | over the past two decades there has arisen a new branch of biology--space biology. this short review is devoted to a discussion of its achievements. it considers the results of research in the area of gravitation biology, and an account is made of studies in those areas of radiobiology which have relevance to the study of the cosmos. there is a brief summary of the results of the search for the upper and lower limits of the biosphere, and information is presented regarding the measures employed ... | 1979 | 398718 |
| biosynthesis and mutasynthesis of aminocyclitol antibiotics. | | 1979 | 398906 |
| mutational biosynthesis of new antibiotics. | | 1979 | 386923 |
| biosynthesis of sisomicin and gentamicin. | | 1979 | 398907 |
| target substances of some antifungal agents in the cell membrane. | copiamycin, an antifungal antibiotic, exhibits antimicrobial activity against a few bacteria in addition to a wide variety of fungi. the methanol extract of sarcina lutea, one of the most susceptible bacteria, was found to reverse the antimicrobial activity of copiamycin. the reversing activity was associated with the phospholipid fraction of the bacteria. the s. lutea phospholipids also reversed the activities of azalomycin f and miconazole, but not that of clotrimazole. the effects of authenti ... | 1978 | 400824 |
| a new and improved assay for endo-beta-n-acetylglucosaminidase. | | 1977 | 401633 |
| biosynthesis of n-methyl-l-glucosamine from d-glucose by streptomyces griseus. | biosynthesis of n-methyl-l-glucosamine moiety of streptomycin from d-glucose by streptomyces griseus was studied. a mixture of d-[1-(14) c] glucose and d-[6(-3)h]glucose was given to the culture of s. griseus. the 3h/14c ratio found in n-methyl-l-glucosamine further supports a mechanism that the conversion of d-glucose to l-hexose is carried out without scission of carbon skeleton. when d-[1-14c]glucose and d-[3-3h]glucose were used, the fall of 3h/14c ratio in n-metyl-l-glucosamine showed that ... | 1977 | 410456 |
| cleavage of adenosine 5'-monophosphate during uptake by streptomyces griseus. | unlabeled adenine brought about a (delayed) decrease in radioactivity that had been taken up by phosphate-limited resting cells of streptomyces griseus from [14c]adenine-labeled adenosine 5'-monophosphate (amp). inorganic phosphate, on the other hand, stimulated adenine uptake from amp, presumably by activating an energy-dependent active transport mechanism. unlabeled phosphate rapidly diluted the uptake of radioactivity from [32p]amp. adenine inhibited uptake of [32p]amp but not that of [32p]or ... | 1977 | 410793 |
| purification and specificity of carboxypeptidase from streptomyces griseus k-1. | a carboxypeptidase of st. griseus k-1 (cpase s) was found to possess the specificities of both mammalian pancreatic cpase a and b. three adsorbents for affinity chromatography were prepared by coupling l-leu, d-leu, and d-arg with ch-sepharose 4b. d-arg-ch-sepharose and l-leu-ch-sepharose retained the purified cpase s but d-leu-ch-sepharose did not. the activities of cpase s toward cgl and bga were eluted in the same position. cpase s migrated as a single band on polyacrylamide gel electrophores ... | 1977 | 410802 |
| thioltrypsin. chemical transformation of the active-site serine residue of streptomyces griseus trypsin to a cysteine residue. | the active-site serine residue of streptomyces griseus trypsin was converted to a cysteine residue, and the product, thioltrypsin, was purified through two chromatographic steps with organomercurial-sepharose and soybean trypsin inhibitor-sepharose as specific adsorbents. the purified preparation of thioltrypsin was found to contain a single residue of cysteine and to react with almost equimolar amounts of normality titrants. it exhibited only traces of catalytic activity toward typical trypsin ... | 1977 | 410803 |
| microbial transformations of glaucine. | | 1977 | 401819 |
| interaction of granaticin b with the transcription system of bacillus subtilis. | the interaction of granaticin b, a quinone antibiotic produced by streptomyces granaticolor, with some biologically important bivalent metal ions, dna and atp was demonstrated spectrophotometrically. the activity of isolated rna polymerase was higher when the dna of phage sp 50 served as template than with dna isolated from bacillus subtilis. granaticin b inhibited in vitro rna synthesis, similarly to certain other antibiotics (the inhibition was three times lower than that caused by actinomycin ... | 1977 | 411719 |
| lipids of streptomyces griseus. | | 1977 | 411737 |
| effect of age on the major phospholipids of streptomyces griseus. | | 1977 | 411740 |
| restriction endonuclease map of euglena gracilis chloroplast dna. | a physical map of the euglena gracilis chloroplast genome has been constructed, based on cleavage sites of euglena gracilis chloroplast dna treated with bacterial restriction endonucleases. covalently close, circular chloroplast dna is cleaved by restriction endonuclease sali into three fragments and by restriction endonuclease bamhi into six fragments. these nine cleavage sites have been ordered by fragment molecular weight analysis, double digestions, partial digestions, and by digestion studi ... | 1977 | 403933 |
| [genetic homologies among staphylococcus aureus and related organisms (author's transl)]. | | 1977 | 411959 |
| studies on the ionophorous antibiotics. xi. the artifacts and the degradation products of lysocellin. | lysocellin is a new polyether antibiotic produced by streptomyces cacaoi var. asoensis and is characterized as a broad spectrum ionophore having a higher complexation affinity for divalent cations than for monovalents and also having an ability to transport biological amines. the structures of two artifacts designated l1 and m1 have been elucidated based on spectral evidence, and the formation mechanism of these compounds was discussed with respect to the reactivity of the antibiotic. in additio ... | 1977 | 412819 |
| effect of barbital on the biosynthesis of streptomycin in streptomyces griseus. | | 1977 | 412820 |
| [effect of the aeration and agitation states on tetracycline biosynthesis in semiproduction-capacity apparatus]. | the results of the experiments on determination of the effect of aeration and agitation conditions on biosynthesis of tetracycline in the apparatus of semi-production capacity are discussed. it was shown that the antibiotic production level was not connected with the rate of oxygen solution expressed in the sulphite numbers, i.e. this parameter cannot be used as a scaling-up criterion. accumulation of the antibiotic in the fermentation broth depended on the volume of the air supplied for aeratio ... | 1977 | 413474 |
| production of geosmin in fermentors and extraction with an ion-exchange resin. | a method for growing streptomyces griseus lp-16 in fermentors and extracting and purifying geosmin, using an ion-exchange resin, is described. | 1977 | 413484 |
| acetylated methylmannose polysaccharide of streptomyces. | a polysaccharide composed of 3-o-methyl-d-mannose and d-mannose in a molar ratio of approximately 10:1 and containing 3 to 4 esterified acetyl residues has been isolated from streptomyces griseus. this acetylated methylmannose polysaccharide (ammp) is similar to the methylmannose polysaccharide (mmp) of mycobacterium smegmatis (gray, g. r., and ballou, c. e. (1971) j. biol. chem. 246, 6835-6842) in its size and composition, the absence of acidic or basic groups, and the lack of a reducing end. i ... | 1977 | 404290 |
| anhydrotrypsin and trypsin: subtle difference in the active-site conformations detected by chemical modification and cd spectroscopy. | the reactivities of the active-site histidine residue in bovine trypsin and its anhydro-derivative, as well as in streptomyces griseus trypsin and its anhydro-derivative have been compared. the reactivity with tlck was found to be lost in both of the anhydrotrypsins. on the other hand, alkylation by iodoacetamide either in the presence or absence of 1-methylguanidine proceeded faster in anhydrotrypsins than in trypsins. these differential responses to alkylating reagents are discussed in terms o ... | 1977 | 405379 |
| 13c-nmr studies on the biosynthesis of aurodox (antibiotic x-5108). | | 1979 | 381271 |
| controlled biosynthesis of neoviridogriseins, new homologues of viridogrisein. i. taxonomy and fermentation. | neoviridogriseins, new homologues of viridogrisein, were produced with viridogrisein and griseoviridin by streptomyces sp. p8648 which was identified as a strain of streptomyces griseoviridus. | 1979 | 381272 |
| rapamycin (ay-22,989), a new antifungal antibiotic. iv. mechanism of action. | rapamycin, an antifungal antibiotic produced by streptomyces hygroscopicus showed a strong candicidal activity, which could not be reversed by sterols. it has no effect on efflux of k+, pi ir u.v. absorbing materials and cell permeability of candida albicans. thus, in its action it differs from the polyenes. mechanism of action of rapamycin appears to be different from many known antifungal agents. in c. albicans, rapamycin at the minimum growth inhibitory concentration inhibited: 1) phosphate i ... | 1979 | 381274 |
| on the structure of the teichoic acid from the cell wall of streptomyces antibioticus 39. localization of the phosphodiester linkages and elucidation of the monomeric units structure by means of 13c-nuclear-magnetic-resonance spectroscopy. | the cell walls of streptomyces antibioticus 39 contain a glycosylated poly(glycerol phosphate), in which the repeating monomeric unit is o-alpha-d-galactopyranosyl-(1--3)-o-2-acetamido-2-deoxy-beta-d-galactopyranosyl-(1--1)-glycerol monophosphate. the localization of the phosphodiester linkages between hydroxyl groups at positions 2 and 3 of the adjacent glycerol residues and the structure of the glycoside were established by 13c nuclear-magnetic-resonance spectroscopy. the spectral data are als ... | 1979 | 393509 |
| influence of antimicrobial agents on contamination and chlortetracycline production. | the possibility of shortening the thermal sterilization time for cultivating media was demonstrated in chlortetracycline fermentation with an industrial strain of streptomyces aureofaciens. the medium was artificially contaminated with a mixture of eight strains of g+ and g- bacteria isolated from contaminated industrial fermentors, and the following chemical agents, either alone or in combination, were added: formaldehyde, phenol. dimethylformamide, p-aminosalicylic acid and nitrofurazone. dime ... | 1978 | 414978 |
| 2-amino-5-methyl-5-hexenoic acid, a methionine analog produced by streptomyces sp. mf374-c4. | 2-amino-5-methyl-5-hexenoic acid (amha), a new methionine analog, was isolated from a fermentation broth of streptomyces sp. mf374-c4 based on its reversal of the effect of n-methyl-n'-nitro-n-nitrosoguanidine (mnng) in a test system that determines the size of growth zones of revertants (his+) of salmonella typhimurium ta1535. amha also inhibited growth of the tester strain in a synthetic medium. these amha activities were abolished by methionine. the incidence of spontaneous streptomycin-resis ... | 1979 | 393683 |
| volatile substances from actinomycetes: their role in the odor pollution of water. | | 1979 | 396107 |
| a new restriction endonuclease from streptomyces albus g. | | 1978 | 415144 |
| antibiotics from marine microorganisms with reference to plasmid involvement. | | 1979 | 396364 |
| [lysis of the cell walls of streptococcus group a by streptomyces griseus pronase]. | the effect of streptomyces griseus pronase on streptococcus group a cell walls was studied. cell walls were shown to be lysed by pronase, the lysis level being dependent on the molarity of the potassium-phosphate buffer used. with an increase in the buffer molarity from 0.005 m to 0.05 m lysis of cell walls decreased from 70-80% to 30%. by deae-cellulose chromatography lysates were separated into two fractions the first of which contained a group specific polysaccharide. a preparative method of ... | 1978 | 416431 |
| [biological effects of distamycin a]. | in the present review are examined different biological effects of distamycin a, an antibiotic produced by streptomyces distallicus. this drug is moderately active on bacteria but it is strongly active on dna viruses and on some oncornaviruses, moreover it possesses antineoplastic and antimitotic activity at high doses at which it can result tossic and cause immunodepression. | 1978 | 398699 |
| [studies on the glycopeptides of canine prothrombin]. | after degradation of canine prothrombin by the complex of streptomyces griseus proteases four glycopeptides were obtained. each of them contained aspartic acid, hexosamines, mannose, galactose and sialic acids. canine prothrombin contains two or three carbohydrate chanins, which are bound to aspartic (asparagine) residues. microheterogenity of the carbohydrate chains of canine prothrombin was found. | 1977 | 407947 |
| ribosomal ribonucleic acids from streptomyces griseus. | nucleic acids from streptomyces griseus 178 were isolated during cultivation. after their fractionation on a column of methylated serum albumin adsorbed on kieselguhr, the 16 s and 23 s rna were isolated. to characterize rnas their sedimentation coefficients, tm and nucleotide composition were determined. during cultivation of s. griseus 178 rrna level reaches two maximum peaks and the production of streptomycin influences nucleic aicds of the producer organism. | 1977 | 409702 |
| [interaction of streptidin-dependent actinomyces streptomycini (streptomyces griseus) mutants no. 170 and 145 with mutants having blocks at various stages of streptomycin biosynthesis]. | in complementation analysis of low active streptidine dependent strains of act. streptomycini, 170 and 145 with mutants having different blocks in biosynthesis of streptomycin it was found that these strains were the donors of some thermostable substances and could reduce the biosynthesis of streptomycin in the mutants having impairements in biosynthesis of streptidine and streptobiosamine, as well as in a number of strains with unknown blocks. it is supposed that the substances produced by muta ... | 1978 | 417668 |
| metabolic products of microorganisms 167. cyclopaldic acid from aspergillus duricaulis. 1. production, isolation and bioloical properties. | in the course of a screening for new metabolites from fungi we isolated a substance with antimicrobial activity from cultures of aspergillus duricaulis (cbs 481.65) (tü 679). it was antagonized by putrescine, spermidine, spermine, arginine, citrulline, lysine, ornithine, in higher concentration by aspraagine and glutamine too. the effect of ethylenediaminetetraacetate on the susceptibility of streptomyces viridochromogenes (tü 57) and bacillus subtilis atcc 6051 to this antibiotic has been studi ... | 1978 | 417692 |
| [the role of teichoic acids in the regulation of biochemical processes in microorganisms]. | the role of cell wall teichoic and lipoteichoic acids in the regulation of activity of some autolytic enzymes and in ion exchange, the role of lipoteichoic acids in biosynthesis of cell wall teichoic acids and some other functional features of these compounds in the bacterial cell are discussed. | 1978 | 417744 |