| non-specific and specific active immunotherapy in a b16 murine melanoma system. | conflicts amongst reports concerning the efficacy of both nonspecific and specific attempts at immunotherapy may be ascribed to different animal models utilizing tumors of different immunogenicity. we have selected the b16 mouse melanoma model as the example of a spontaneously occurring neoplasm that is histocompatible with the host and does have tumor-associated antigens. attempts to alter tumor growth or survival with nonspecific active immunotherapy as well as with specific active immunothera ... | 1979 | 480953 |
| [growth of a propionibacterium shermanii culture under varying aeration conditions]. | the effect of aeration of growth of propionibacterium shermanii bkm b-103 was studied. aeration was found to stimulate growth of the culture in a complex medium containing sodium lactate as a source of carbon and energy. when the concentration of oxygen in the medium was raised, the economic coefficient increased, the ks value for lactate assimilation decreased, and the value of mum did not change. | 1979 | 481279 |
| activation of human monocytes by mediators from lymphocytes stimulated with corynebacterium parvum. | human monocytes activated in vitro by lymphokine-containing supernatants of autologous or allogeneic lymphocytes stimulated in vitro by corynebacterium parvum (cp) expressed increased ability to suppress dna-synthesis in a human tumour cell line. monocyte activation was not dependent on in vitro differentiation of monocytes, enhanced cytostatic ability being observed at all stages of in vitro differentiation. the lymphokine-induced cytostatic ability was not affected by intensive washing and try ... | 1979 | 484237 |
| monocytes and macrophages in malignant melanoma iv. effects of c. parvum on monocyte function. | assays for the capacity of peripheral-blood monocytes (a) to mature in vitro into macrophages, (b) to reduce nitro-blue tetrazolium (nbt) and (c) to lyse antibody-coated human group a red cells, were applied to a group of 82 patients with histologically proven malignant melanoma. in patients with micrometastatic disease there was an enhancement of red-cell lysis and nbt reduction, suggesting that their monocytes are in some way "activated", whereas nbt reduction was suppressed in those with over ... | 1979 | 486312 |
| fc receptor-bearing and phagocytic cells in syngeneic tumours of c. parvum- and carrageenan-treated mice. | | 1979 | 486317 |
| effects of repeated corynebacterium parvum and bcg therapy on immune parameters: a weekly study of melanoma patients ii. changes in serum immunoglobulins and lymphoid cell subpopulations. | two groups, each of eight patients, with metastatic melanoma were immunized with either c. parvum or bcg at 3-weekly intervals. the serum igg, iga, igm levels, e-rosettes, ea-rosetts, sig-bearing cells, 'null cells' and lymphocyte blastogenesis at four pha concentrations were assayed, before immunization and weekly thereafter for 8 weeks. the pre-immunization e-rosette values were significantly reduced, with a significant increase in 'null cell' values and ea-rosette percentages, when compared w ... | 1979 | 487647 |
| the reversal of surgically induced reticuloendothelial depression. | the function of the reticuloendothelial (re) system may be depressed by surgical operation. as this system is an important defence mechanism against tumour growth and metastasis, it would be an advantage if this depression could be prevented by the administration of a suitable agent. the agents studied in this case were levamisole, corynebacterium parvum (c. parvum) and glucan. as a measure of re function a carbon clearance test was performed in rats, 1 hr after partial colectomy, animals having ... | 1979 | 487654 |
| immunotherapy of a murine ovarian carcinoma with corynebacterium parvum and specific heteroantiserum. i. activation of peritoneal cells to mediate antibody-dependent cytotoxicity. | immunotherapy with a combination of corynebacterium parvum and specific heteroantiserum is significantly more effective than treatment with either single agent in prolonging the survival of mice that have recevied an i.p. injection of syngeneic murine ovarian carcinoma (mot) cells. invitro, a combination of c. parvum-activated peritoneal cells and specific heteroantiserum has proven significantly more effective than either single component in destroying 51cr-labeled mot cells in the absence of c ... | 1979 | 489967 |
| chemoimmunotherapy for meningeal melanocytoma of the thoracic spinal cord. report of a case. | | 1979 | 490860 |
| anaerobic bacteria in late infections following orthopedic surgery. | twelve patients with late anaerobic infection following hip or knee arthroplasty or tibia osteosynthesis are presented. peptococci (peptococcus asaccharolyticus, p. magnus, p. prevotii) and peptostreptococci (peptostreptococcus micros, p. anaerobius, p. parvulus) were present in nine of the cases. anaerobic gram-positive or -negative rods were found in three cases (propionibacterium acnes, actinomyces israelii, and bacteroides fragilis). antibodies against the isolated bacteria were detected in ... | 1979 | 492071 |
| recovery of anaerobic bacteria from pediatric patients. a one-year experience. | during 1975, 115 speciments from the soft tissues, body fluids, and purulent sites in 105 pediatric ward and nursery patients were submitted to the clinical laboratory for anaerobic study, and 75% of the cultures were positive. in most instance specimens were collected in anaerobic transport tubes containing peptone yeast extract and glucose. in the laboratory, samples were processed by the anaerobic holding jar method. nineteen different anaerobic organisms were isolated; the average number of ... | 1979 | 495592 |
| pharmacokinetics of phenytoin and liver cytochrome p-450 levels in mice after single doses of corynebacterium parvum [proceedings]. | | 1979 | 497584 |
| comparative antitumor effects of corynebacterium parvum, bordetella pertussis, bacillus calmette-guérin, and levamisole alone or in combination with cyclophosphamide in the cad2 murine mammary adenocarcinoma system. | the antitumor efficacy of various immune stimulants [corynebacterium parvum, bordetella pertussis, and bacillus calmette-guérin (bcg)] and levamisole alone or in conjunction with cyclophosphamide (cy) was studied in the cad2 mammary adenocarcinoma system using schedules developed previously with c. parvum and cy. weekly systemic treatment with c. parvum, b. pertussis, or bcg was effective in controlling tumor growth and had equivalent antitumor effects, but weekly treatment (or a single treatmen ... | 1979 | 498111 |
| surgery plus corynebacterium parvum immunotherapy for lewis lung carcinoma in mice. | | 1979 | 499275 |
| effects of whole body irradiation in mice treated with c. parvum. | | 1979 | 499277 |
| lung cancer patients treated with corynebacterium parvum. phase i toxicity study. | | 1979 | 503003 |
| host treatments affecting artificial pulmonary metastases: interpretation of loss of radioactively labelled cells from lungs. | the effect has been examined of various host treatments (c. parvum injection, immunization, thoracic irradiation, cyclophosphamide injection, and anticoagulation) on both lung colony formation and clearance of radioactive cells from the lungs after i.v. injection of tumour cells. two tumour-host models have been used: the non-immunogenic kht tumour in c3h/km mice, and the immunogenic emt6 tumour in balb/c/ka mice. even for the at most weakly immunogenic kht tumour, the number of artificial pulmo ... | 1979 | 508577 |
| [adriamycin-combination chemotherapy with or without immune stimulation by corynebacterium parvum in metastasizing carcinoma of the breast (author's transl)]. | in a randomised, multicentre study the effect of changing the combined cytostatic treatment with vac (vincristin, adriamycin, cyclophosphamide) to fmc (fluorouracil, methotrexate, cyclophosphamide) was tested, as well as the effect of active non-specific immune stimulation with corynebacterium parvum, and compared with a control group. in 59 of 120 patients (49%) in whom the results could be analysed there was a measurable significant regression of tumour size to less than 50% of its initial val ... | 1979 | 510206 |
| macrophage precursors in ovarian carcinoma. | | 1979 | 510998 |
| alterations induced in macrophage and granulocyte-macrophage colony-forming cells by a single injection of mice with corynebacterium parvum. | | 1979 | 513059 |
| chemical and biological properties of a purified lymphoreticular-stimulating fraction of corynebacterium parvum (propionibacterium acnes type i). | a method is described in which washed whole cells of corynebacterium parvum were chemically and enzymatically extracted to remove cytoplasm and cell-wall lipids. the resultant insoluble cell-wall residue possessed lympho-reticular stimulating properties as measured by their ability to increase spleen weight and protect against tumour-cell challenge. analysis of the final product by chromatography and infrared spectroscopy has shown it to consist of carbohydrate and peptidoglycan, both of which a ... | 1979 | 513108 |
| [synthesis of organic acids by immobilized propionic bacteria in the flow system and stabilization of the process]. | the capacity of immobilized cells of propionic bacteria to synthesize organic acids was examined. propionibacterium shermanii cells incorporated into polyacrylamide gel were capable to synthesize propionic, acetic and pyruvic acids in the flow system. as a carbon source glucose, lactate-na or whey lactose was used. the greatest amount of the acids was synthesized with the use of lactate-na. the life-time of the biocatalyst (immobilized cells) can be increased by its reactivation with a nutrient ... | 1979 | 514985 |
| [the bacterial contamination of epidural catheters with areobe and anaerobe germs in obstetrics (author's transl)]. | following anaesthesia the tips of 105 epidural catheters were investigated for aerobe and anaerobe bacteria. in one case aerobe germs (staphycoccus epidermidis) and in three cases anaerobe germs (propioni-bacteria were isolated. in two further cases aerobic growth was suspected but not confirmed in sub-cultures. the contamination rate was therefore 0.9% aerobe and 4.8% by anaerobe bacteria. even in the face of a clinically uncomplicated course the presence of bacteria on the catheter tip is cons ... | 1979 | 520786 |
| the facilitation of tumour growth in the lung by cyclophosphamide in artificial and spontaneous metastases models. | | 1979 | 527634 |
| active, specific immunotherapy of murine leukemia. ii. adjuvant effect of corynebacterium parvum. | we have previously shown that irradiated lstra cells (lx) were immunogenic and could prolong survival of mice bearing lstra tumors. this study demonstrated that addition of corynebacterium parvum to the lx dramatically improved the strength of tumor immunity. in pretreatment-challenge experiments, c. parvum augmented the immunogenicity of 10(6) lx given intradermally, with an optimum dose of 0.14-1.4 microgram c. parvum per mouse. in therapy experiments (intraperitoneal vaccine treatment after t ... | 1979 | 528079 |
| action of cancer chemotherapy-radiotherapy on spleen hypertrophy response to corynebacterium parvum adjuvant therapy. | | 1979 | 536279 |
| anaerobic isolates in chronic suppurative otitis media. | | 1979 | 538681 |
| [study and differentiation of some hydrolases active on triglycerides and esters, in anaerobic bacteria, using gas liquid chromatography (author's transl)]. | anaerobic bacteria are classified using presence or absence of phospholipase and lipase, among other criteria. techniques are described for the qualitative and quantitative detection of bacterial esterases (carboxylic ester hydrolase) and lipases (triacylglycerol acyl hydrolase) endo- or -exocellular, using gas liquid chromatographic method. results with representatives anaerobic species are briefly presented. | 1979 | 539692 |
| biotyping of propionibacterium acnes isolated from normal human facial skin. | biochemical and serological characteristics of 128 strains of propionibacterium acnes isolated from the facial skin of healthy japanese volunteers were compared with the three standard strains of the american type culture collection, atcc 6919, 11827, and 11828. accordingly, the isolated strains of p. acnes were classified into five biotypes (b1 to b5) on the basis of fermentation tests of ribose, erythritol, and sorbitol. two serotypes were distinguished by the agglutination test. p. acnes belo ... | 1979 | 539817 |
| effects of intravenous injection of two different strains of corynebacterium parvum in the mouse. | a strain of c. parvum, cn6134, known to have antitumour activity, caused thrombosis in the sites where the organism is phagocytosed. it bound to macrophages in vitro and activated the alternate pathway of complement. a strain of c. parvum, cn5888, which fails to show antitumour activity, did not show thrombosis. it did not bind to macrophages or activate guinea-pig complement. it did, however, cause marrow infarction which seems to result from a diminished clearance of the organism from the circ ... | 1979 | 540104 |
| growth of cutaneous propionibacteria on synthetic medium; growth yields and exoenzyme production. | | 1979 | 541303 |
| prolonged effect of corynebacterium parvum stimulation on granulopoiesis. | an intravenous injection of 548 microgram of killed corynebacterium parvum (c. parvum) into c57bl mice led to a significant increase in the number of both committed stem cells (gm-cfc) and proliferating cells of bone marrow granulocytic series. this increase appeared 24 h after the injection of the bacterium, persisted in varying levels until the 4th week and was accompanied by a marked and prolonged granulocytosis. the proportion of gm-cfc in dna synthesis was found to be increased 1, 2, and 5 ... | 1979 | 542797 |
| comparison of three methods of susceptibility testing of bacteroidaceae, peptococcaceae and other anaerobes to doxycycline. | the in vitro activity of doxycycline against 147 strains of gram-positive and gram-negative anaerobes was determined by broth dilution, agar dilution and agar diffusion tests. the strains were isolated from clinical specimens in 1977. saccharolytic bacteroides strains (39 b. fragilis, 6 b. thetaiotaomicron, 4 b. vulgatus) showed broth dilution mics of less than or equal to 0.0625-4.0 microgram/ml after 5 h incubation and mics of less than or equal to 16 microgram/ml after 15 h incubation at 37 d ... | 1979 | 543353 |
| protection of mice against endotoxin-induced liver damage by anti-inflammatory drugs. | mice were injected with corynebacterium parvum, which induces multiple granulomas in liver and renders animals hyper-reactive to the lethal effect of bacterial lipopolysaccharide (lps). such animals when challenged with lps developed also extensive liver parenchymal cell damage as estimated by elevated blood asparate transaminase levels and a hypoglycaemia. treatment with indomethacin, hydrocortisone, dexamethasone, promethazine, metiazinic acid and (+)-catechin ameliorated the liver damage. hyd ... | 1979 | 546179 |
| activation of pleural macrophages by intrapleural application of corynebacterium parvum. | a single ipl injection of 0.25 mg cp into cba mice led to accumulation of macrophages in the pleural cavity, but it did not influence res as an injection given iv ipl cp caused a three-to-five-fold increase in the number of nucleated cells in the pleural cavity which persisted at least 14 days. of these cells 86% were macrophages as shown by their esterase activity. less than 30% of cells from the pleural cavity of normal mice were esterase positive. macrophages from the pleural cavity of cp-tre ... | 1979 | 547729 |
| role of mononuclear phagocytes in decreased hepatic drug metabolism following administration of corynebacterium parvum. | | 1979 | 551956 |
| studies on propionibacterium isolated from paddy soils. | the anaerobic bacteria occurring in paddy soils sampled from fields belonging to the rice research station at ohmagari in akita prefecture, japan, were surveyed from may to november, 1974. irregularly shaped rods predominated which were found to be gram-positive, strictly anaerobic, catalase-positive and not heat-resistant; populations were estimated at 10(5)--10(-7) cells/g dry soil throughout the entire survey period. on the basis of their morphological and physiological characteristics these ... | 1979 | 552815 |
| [in vitro sensitivity of corynebacterium acnes (propionibacterium acnes) to meclocycline, clindamycin, erythromycin and neomycin]. | | 1979 | 554810 |
| biosynthesis of porphyrins and related macrocycles. part 9. biosynthesis of the corrin macrocycle of vitamin b12 including the sterochemistry of c-methylation in ring. | | 1977 | 556734 |
| biosynthesis of porphyrins and related macrocycles. part 10. vitamin b12: biochemical derivation of cobyrinic acid from uroporphyrinogen iii. studies with corresponding ring c methyl heptacarboxylic prophyrinogen, and proof of seven intact methyl transfers. | | 1977 | 556735 |
| corynebacterium parvum as an immunotherapeutic agent in an ovarian cancer model. | corynebacterium parvum, an anaerobic diptheroid, has been demonstrated to be therapeutic in several tumor models by stimulating immunologic defenses. formalin-killed c. parvum was investigated in the present study as an immunotherapeutic agent in the treatment of murine ovarian cancer, a model that closely simulates the activity of clinical disease. c. parvum successfully prolonged survival in murine ovarian cancer and its effectiveness improved with increasing dosage. the efficacy of c. parvum ... | 1977 | 560124 |
| [anerobic corynebacteria propionibacterium as immunostimulators and antioncogenic factors]. | | 1977 | 564299 |
| antibodies that bind biotin and inhibit biotin-containing enzymes. | | 1979 | 571508 |
| [antitumor effect of corynebacterium parvum]. | the protective effect produced by pre-treatment with c. parvum is studied in two syngeneic tumours: mammary carcinoma in c3h mice and lymphosarcoma in xvii mice. the best anti-tumour effect is obtained when both c. parvum and tumour cells are injected by the same route, which, however varies for the two tumours. the study of the recovery of c. parvum protective effect after its suppression by a sub-letal dose of x-irradiation shows that, in the c3h mice, it is parallel to the restoration of the ... | 1977 | 576780 |
| use of litmus milk agar for presumptive identification of cutaneous propionibacteria. | presumptive identification of cutaneous propionibacteria can be achieved with litmus milk agar as medium. | 1977 | 577927 |
| active experimental inhibition of cancer by corynebacterium parvum. | it has been demonstrated that 5 microgram corynebacterium parvum (mérieux, lyon) applied i.v. once weekly in mice of the strain c 3 h x swiss albino f 1 after three pregnancies effectively inhibits tumour growth. at this time those mice are 100% at risk. the appearance of tumours in the treated group is significantly delayed. | 1977 | 579449 |
| [use of propiono-acidophilus milk in the complex treatment of intestinal dysbacteriosis in infants with staphylococcal infections and sepsis]. | antistaphylococcal properties of the new lactic acid mixture propionic acidophilic milk (pam) against the microbe isolated from feces of children suffering from staphylococcal sepsis were comparatively studied by the dilution and diffusion methods. pam was found to have more pronounced antimicrobial properties against pathogenic staphylococcus than acidophilic milk and kefir. it is recommended to include pam into the diet of children with intestinal dysbacteriosis. | 1979 | 583187 |
| c. parvum immuntherapy of transplanted rat tumours. | c.parvum (wellcome cn 6134) has been tested for tumour suppression against a range of syngeneically transplanted rat tumours, both carcinogen-induced and of spontaneous origin. subcutaneous growth was not prevented by distant subcutaneous or intravenous injection of the preparation, although growth rates were sometimes depressed or accelerated. in contrast, c. parvum injected in admixture with tumour cells consistently suppressed their growth and with highly immunogenic tumours induced systemic ... | 1977 | 591131 |
| presumptive diagnosis of anaerobic bacteremia by gas-liquid chromatography of blood cultures. | by quantitative gas-liquid chromatography of glucose-containing blood cultures at the moment of first signs of growth, a presumptive diagnosis of anaerobic bacteremia could be made in 24 out of 26 cultures yielding obligate anaerobes upon subsequent culture. with bacteroides sp. (20 strains isolated), elevated levels of isovaleric acid (greater than or equal to 0.1 mumol/ml) and/or succinic acid (greater than or equal to 5 mumol/ml) were detected in the medium used. an exception to this were two ... | 1977 | 591630 |
| [sensitivity of sherman's propionic acid bacilli to antibacterial preparations and vitamin b12 synthesis]. | sherman propionic acid bacilli were sensitive to benzylpenicillin, ampicillin, ceporin, tetracyclines, oleandomycin, oletetrin, tetraolean, sigmamycin, levomycetin and furadonine. methicillin, oxacillin, monomycin, kanamycin, polymyxin and furazolidone had an insignificant effect on the above organism. the subbacteriostatic concentrations of methicillin, oxacillin, streptomycin, monomycin, kanamycin, neomycin, tetraolean, sigmamycin, polymyxin m and ristomycin increased the biosynthesis of vitam ... | 1977 | 596839 |
| effect of corynebacterium parvum on peripheral blood platelets. | the level of peripheral blood platelets was determined after i.v. injection of corynebacterium parvum in normal c57bl mice and in those bearing the lewis lung carcinoma. twenty minutes after injection of a formalin-killed active strain (cn6134, (cn6134, which inhibited tumour metastases) or a killed inactive strain (cn 5888, which did not inhibit metastases) the number of circulating blood platelets was reduced by 50%. the level of platelets returned to control values by 8 h after the active, an ... | 1977 | 597475 |
| effect of corynebacterium parvum on subcutaneous and skeletal methylcholanthrene sarcoma implants. | | 1977 | 598063 |
| combination chemo-immunotherapy with adriamycin in experimental tumor systems. | | 1977 | 598392 |
| [biosynthesis of corriphyrins - a new branch of metabolism of tetrapyrrole compounds]. | experiments were carried out to study biosynthesis of tetrapyrrole pigments--corriphyrins which are methylated reduced derivatives of uroporphyrin iii. it was shown that the pattern of corriphyrin synthesis was close to that of vitamin b12. the mutant strain of propionic bacteria which was active in the vitamin b12 formation synthesized significantly more corriphyrins than the original strain. the corriphyrin synthesis was stimulated by delta-aminolevulinic acid and methionine and was inhibited ... | 1977 | 600920 |
| [immunological problems in cancer]. | | 1977 | 601267 |
| the complications of intravenous corynebacterium parvum infusion. | 100 intravenous infusions of corynebacterium parvum were given to thirty-six patients at a dose of 5 mg/m2. fever and rigors occurred in all patients but these acute side effects were acceptable to all but two patients. seventeen patients suffered a delayed fall in blood pressure, which was marked in eight of them but was generally well tolerated. one patient died 18 hr after infusion from extensive myocardial infarction. herpes labialis complicated the first infusion in nine instances, which ma ... | 1977 | 606444 |
| comparative studies on the cell wall composition of some anaerobic coryneforms of varying lympho-reticular stimulatory activity. | anaerobic diphtheroids possessing lympho-reticular stimulatory properties may differ considerably in their peptidoglycan composition. spleen weight-increasing activity of strains directly parallels their antitumour properties. p. granulosum strains, inactive in assays for lympho-reticular stimulation, appear to have a higher cell wall alanine content than most of the p. acnes and p. avidum strains tested. two p. acnes strains, however, had equivalently high alanine ratios and were stimulatory. t ... | 1977 | 608497 |
| studies using labelled c. parvum preparations in mice. | the in vivo distribution of 125i and fluorescein labelled c. parvum, after local and systemic injection mice has been studied. after i.v. injection large amounts were found in the liver, spleen and lungs, with less in the lymph nodes. the distribution after s. c. injection was more restricted; the bulk of inoculum was retained at the injection site and draining lymph node, and only small amounts were found in the liver and spleen. c. parvum was found in bone marrow, but not peritoneal cells afte ... | 1977 | 608498 |
| distribution of technetium-99m labeled corynebacterium parvum in normal and tumor bearing mice. | | 1977 | 608499 |
| distribution of 3h-thymidine-labelled c. parvum in mice. | little is known of the localisation of injected corynebacterium parvum. we therefore developed a method of radiolabelling this vaccine and determined its distribution in the mouse. live c. parvum (wellcome, strain cn 6134) was grown in the presence of 3h-thymidine and subsequently killed by formalin. a high activity, 1-6 x 10(5) cpm/0.1 ml of a 7 mg dry weight/ml concentrated suspension, was obtained. its biological properties (hepatosplenomegaly and antitumour effects) were similar to those of ... | 1977 | 608501 |
| further studies on the enhancement of the antibody response in vitro by c. parvum. | we have shown previously that spleen cells from mice pre-treated with c. parvum give enhanced antibody response when cultured in the presence of thymus-dependent antigens. in order to establish if c. parvum has a similar effect when added in vitro, spleen cells from untreated mice were cultured with c. parvum in marbrook chambers with sheep erythrocytes (srbc) as antigen. at concentrations of 35-140 microgram/ml/20 x 10(6) spleen cells c. parvum enhanced, but at higher concentrations suppressed ... | 1977 | 608502 |
| antibody responses, antitumour antibodies and ig class and subclass levels in c. parvum treated mice. | this paper summarises the results of extensive studies performed in our laboratory during the past three years aimed at establishing the effect of c. parvum administration on humoral immunity in normal mice and mice previously challenged with viable mc fibrosarcoma cells. this treatment was found to (a) increase the response to thymus dependent and independent antigens, (b) result in the production of circulating immunoglobulins which bound to tumour cells and syngeneic tissues in vitro, and (c) ... | 1977 | 608503 |
| monocytosis-inducing activity (mia) of serum in corynebacterium parvum treated mice. | intravenous injection of 548 microgram of c. parvum (lot 0407, mérieux laboratories, france) into c57bl mice produced rapidly appearing monocytopenia which was followed by marked and prolonged monocytosis after the third day. the serum of these animals, collected during the monocytopenic but not the monocytosis phase, showed monocytosis inducing activity (mia) as was demonstrated by the intravenous injection of the serum into normal test mice. serum from normal untreated mice or from mice given ... | 1977 | 608504 |
| cell mediated immunity to corynebacterium parvum and its role in tumor control. | the injection of c. parvum provokes a transient cell-mediated immune response to its own bacterial antigens. during this period a subcutaneous challenge dose results in the appearance of a characteristic delayed type hypersensitivity (dth) skin reaction. if a suspension of syngeneic tumor cells is included in this challenge, they are destroyed in immune but not control animals. similarly, solid tumors of limited size can be made to regress under these conditions. adoptive transfer studies reveal ... | 1977 | 608505 |
| potentiation of anamnestic humoral responses by a single intravenous injection of corynebacterium parvum (c. parvum) in adult rabbits. | | 1977 | 608507 |
| effect of corynebacterium parvum on the immune response of the guinea pig to ovalbumin. | | 1977 | 608508 |
| in vitro anti-tumour properties of peritoneal exudate cells of conventional germ-free and stimulated mice. | adherent cells of peritoneal exudates were obtained from conventional, or from germ-free mice or from mice having received in intraperitoneal infection of a variety of phlogogenic substances such as corynebacterium parvum (c. parvum) (mérieux) 500 microgram, thioglycolate (difco) 3 ml, bayol (esso) 0.50 ml + 0.50 ml culture medium, glycogene 1.2 ml. the cytotoxic properties adherent cells were studied in vitro by the chromium release technique (crt) and their cytostatic properties by the inhibit ... | 1977 | 608509 |
| effects and mode of action of corynebacterium parvum on murine tumour metastases. | the effects of corynebacterium parvum (c. parvum) on lewis lung tumour metastases have been studied. i.v. and i.p., but not s.c. c. parvum significantly reduced pulmonary metastases. when combined with excision of the primary tumour, c. parvum given not more than 2 days before caused slight reduction of metastases, but when given earlier, it caused significant reduction and some mice were cured. metastases were increased by silica or cortisone acetate but were unaffected by trypan blue. the anti ... | 1977 | 608510 |
| effects of surgery and c. parvum on tumour metastases in mice. | surgical operations depress immune responses. the aim of this study was to observe the effects of operation (amputation) on the growth of the lewis lung tumour and its metastases and to see if c. parvum counteracted them. anaesthesia alone, or with amputation, did not affect the growth of the primary tumour but c. parvum depressed it. anaesthesia did not affect the number of pulmonary metastases but amputation caused significant increase. c. parvum inhibited metastases and completely counteracte ... | 1977 | 608511 |
| a comparison of the antitumour activities of glucans and c. parvum. | after i.v. injection of glucans (yeast cell walls, lentinan and pseudonigeran) into cba mice, the peritoneal macrophages became cytostatic to r1 leukemia cells in vitro, and the mice showed resistance to lung nodule formation by i.v. injected t3 fibrosarcoma cells. the glucans were less active than c. parvum. none of the glucans reproducibly acted as an adjuvant when mixed with irradiated tumour cells; nor did they inhibit the growth of a subcutaneous tumour when injected i.v. or directly into t ... | 1977 | 608513 |
| in vivo transfer of antitumor activity by peritoneal exudate cells from mice treated with c. parvum. | we have investigated whether peritoneal exudate cells (pec) from c. parvum (cp) treated (c3hf/bu mice could transfer in vivo the resistance against a syngeneic fibrosarcoma (fsa). inhibition of tumour development and prolongation of survival of recipients were observed when cp-activated pec were admixed with fsa cells before their intraperitoneal (ip) or subcutaneous (sc) injections into normal mice. the antitumour activity increased with the increase of the ratio of effector to target cells. he ... | 1977 | 608514 |
| effects of c. parvum on radiation response of murine tumors. | the effects of intravenous c. parvum (cp) on the efficacy of fractionated gamma-irradiation in treating a fairly immunogenic fibrosarcoma (fsa) and a weakly immunogenic mammary carcinoma (mdah-mca-4) were studied in c3hf/bu mice. tumors were 8 mm in diameter at the start of irradiation. the fsa was exposed to 500 and the mdah-mca-4 to 750 rads daily for 3.6 or 10 days. the administration of 0.25 mg cp varied from 4 days before to 14 days after the start of irradiation. cp greatly augmented radio ... | 1977 | 608515 |
| chemo-immunotherapy of mouse tumours using cyclophosphamide and c. parvum. | treatment of a chemically induced mouse solid fibrosarcoma using either non-specific (c. parvum 350 mu i.v.), or specific active (s.c. c. parvum mixed with 5.10(5) irradiated tumour cells) immunotherapy, 4 days after a single dose of cyclophosphamide (200 mg/kg) was synergistically more effective than either c. parvum or drug treatment alone. a contributory factor may be that cyclophosphamide pretreatment has been shown to potentiate the specific antitumour immunity that arises from c. parvum in ... | 1977 | 608516 |
| increased sensitivity of c. parvum treated mice to ionizing whole body irradiation. | we have investigated whether c. parvum (cp)-induced stimulation of hematopoiesis affects the survival of mice exposed to the whole body irradiation (wbi). c3hf/bu mice treated with cp exhibit an increased hematopoietic colony forming activity in their spleens and blood, but not in their bone marrow, as determined by the exogenous spleen colony assay. also, cp-treated c3hf/bu as well as cba mice show an increase in their endogenous colonies. this increased hematopoietic activity caused by cp trea ... | 1977 | 608517 |
| experimental evaluation of nonspecific immunostimulation and chemotherapy in the intracranial tumor-bearing fisher rat. | one hundred sixty rats bearing intracranial tumors were divided into groups of 10 to evaluate c-i interval +, effect of chemotherapy alone (bcnu), chemotherapy and c. parvum, and c. parvum alone in the rat tumor model. all therapy was administered intraperitoneally: bcnu -5.2 mg/kg (40% of ld10) on the 9th and 16th post tumor implant day, and c. parvum-1.4 mg regardless of the body weight and the 6th or 23rd day and 6th and 23rd post tumor implant day. an additional group received bcnu on the 9t ... | 1977 | 608518 |
| the effect of pretreatment with corynebacterium parvum on the growth of a chemical induced transplanted murine tumor. | unspecific immunostimulation with or without specific tumor immunotherapy may well contribute to the control of minimal residual cancer. corynebacterium parvum (c.p.) has been shown to raise the level of immunocompetence in tumor bearing animals. experiments are reported, which aim at an evaluation of optimal conditions for an immunostimulation with c.p. prior to the transplantation of balb/c meth a ascitis. using different dosages, routes of injection and schedules of vaccination it was found, ... | 1977 | 608519 |
| effects of bcg and c. parvum treatment on human melanoma xenografts. | cba mice rendered immunodeficient by thymectomy, potentially lethal gamma-irradiation and reconstitution with bone marrow cells were used to grow a wide variety of human tumours as subcutaneous implants. samples of human melanoma obtained at surgery were successfully passaged by transplantation and produced rapidly growing tumours, some of which metastasized to lung, lymph nodes and the para-aortic node; this system was used as a model for the study of immunotherapy of melanoma. preliminary resu ... | 1977 | 608525 |
| the local effect of intravesical corynebacterium parvum in the dog. a preliminary study. | high doses of c. parvum introduced in the bladder of the dog provoke changes in the submucosa. the significance and importance of these modifications should be investigated. | 1977 | 608526 |
| comparison of bcg and c. parvum preparations for adjuvant contact therapy of a rat sarcoma. | | 1977 | 608529 |
| histological changes of intravascular coagulation in mice resulting from intravenous injection of c. parvum. | a histological study was made of liver and other tissues of mice at intervals after injection of c. parvum. as early as 20 min after injection occasional thrombi surrounded by polymorphs were seen in the alveolar capillaries. a general thrombosis rapidly developed in the hepatic vessels with consequent hepatic necrosis. thrombi also developed in splenic vessels. this intravascular coagulation was prolonged, lasting at least 7 days. there was evidence that fresh thrombi were being formed througho ... | 1977 | 608532 |
| effects of corynebacterium parvum vaccine on drug metabolism in the mouse. | mice of the cba strain were given a single intraperitoneal injection of c. parvum vaccine at 50 mg/m2. there followed a progressive increase in liver weight and in the duration of hexobarbitone sleeping time, accompanied by a marked decrease in liver microsomal protein and in cytochrome p-450/mg microsomal protein. that is to say, content of cytochrome p-450 was reduced out of proportion to the apparent loss of endoplasmic reticulum. these changes were paralleled by decreases in microsomal o-dem ... | 1977 | 608533 |
| effects of bcg and corynebacterium parvum on immune reactivity in melanoma patients. | sixteen patients with disseminated melanoma were immunised with either bcg (8 cases) or c. parvum (8 cases) on three occasions at 21 day intervals. blood for assay was taken immediately before the first immunisation and weekly for eight weeks thereafter. total white count tended to increase but little change was seen in lymphocyte and monocyte counts. serum igg increased after bcg but not with c. parvum, serum iga and igm did not alter. the 'e' rosette % did show some increase mainly after c. pa ... | 1977 | 608536 |
| the effect of corynebacterium parvum on human effector cells in peripheral blood. | | 1977 | 608538 |
| in vitro and in vivo effects of corynebacterium parvum on lymphocyte transformation. | using a range of doses the effect of c. parvum on the in vitro response of lymphocytes to pha was studied. c. parvum was not found to be mitogenic itself when added to unfractioned lymphocyte cultures from normal donors and patients with breast cancer, nor did it effect the response to pha in these cultures except at the highest dose (20 microgram/100,000 cells) when it was inhibitory. 21 patients with breast cancer treated with c. parvum were assessed by their in vitro response to pha prior to, ... | 1977 | 608539 |
| the effect of intravenous infusion of corynebacterium parvum on an immune profile of women with breast cancer. | an immunological profile has been measured in 21 patients with advanced breast cancer who were treated with c. parvum (wellcome strain cn6134). thirteen patients received a single i.v. dose of 15 mg of c. parvum and 8 received 4 mg i.v. on 5 successive days. the "profile" was recorded before and 7-10 days after treatment and included measurement of total white count, absolute lymphocyte and monocyte counts, pha response, b and t cell percentages. dncb and mantoux skin tests, immunoglobulin class ... | 1977 | 608540 |
| further studies on the serological effects of c. parvum immunotherapy in cancer patients. | previous serological studies undertaken in our laboratory have shown that the repeated administration of small doses of c. parvum to a small group of cancer patients resulted in a consistant increase in the levels of igg (especially the igg2b subclass), the development of antibodies to c. parvum and the transient appearance of 'rheumatoid factor like' substances in the serum (br. j. cancer, 32, 310, 1975). in the present paper we report the results of similar studies in small numbers of (a) inop ... | 1977 | 608542 |
| first impressions of i.v. c. parvum in patients with solid tumours. | 6 patients with solid tumours (4 malignant melanomas, one fibrosarcoma, one osteogenic sarcoma) received i.v. c. parvum alone or in combination therapy (radiotherapy, levamisole and/or vitamin a). the single doses of c. parvum ranged from 5.0-7.5 mg/m2; no. of doses ranged from 1-8; interval between doses ranged from 2-140 days. 2 patients with malignant melanoma had no measurable disease, one of them (stage i) still has no evidence of disease. the patient with fibrosarcoma appeared to have a mi ... | 1977 | 608543 |
| experience with the quality control testing of c. parvum suspension for clinical trial. | the control of c. parvum suspension presents special problems. the intended application of this preparation is in a therapeutic area where clinicians are accustomed to using defined chemical entities the biological activity of which are usually predictable according to weight of active principle administered, the route and number of doses. but the essential character of c. parvum is that of a vaccine in which the character of the active principle or principles is not clearly identified and can v ... | 1977 | 608544 |
| toxic and immunologic side effects of daily c. parvum-infusion in treatment-resistant cancer patients. | daily increasing intravenous doses of corynebacterium parvum (c.p.) up to 5 mg/m2 i.v. x 10-14 days were given to 6 patients with widespread metastatic neoplastic diseases resistant to radio- and chemotherapy. the immunotherapy treatment-cycles were evaluated for toxic and immunologic side effects and also for possible clinical benefit to the patients. immunotherapy with i.v.-c.p. was moderately well tolerated. subjective discomfort for the patients (headache, chills, nausea) was not better tole ... | 1977 | 608546 |
| a survey of patients' reactions to intravenous corynebacterium parvum therapy. | a survey was made of patients' reactions to high dose (21 mg) corynebacterium parvum therapy given intravenously over four hours. the patients completed a pro forma. rigors were the commonest side effect complained of being present in nearly all patients (24/25) and being very severe in one-third (10/25). other problems were nausea (15/25), vomiting (17/25), bone pain (12/25) and headaches (12/25). one-third also usffered with other less common side effects. overall, patients felt the side effec ... | 1977 | 608547 |
| intra-tumour c. parvum therapy in gastric carcinoma; a pilot study. | in a preliminary and toxicological study, eight patients with carcinoma of the stomach were treated by corynebacterium parvum immunotherapy prior to surgical excision. killed c. parvum suspension was injected at multiple sites directly into the tumour mass with the aid of fibre-optic gastroscope at doses of 1.5 mg, 4 mg or 8 mg. each patient received two injections of the same dose with an interval of 14 days. the second injections were made 14 to 18 days prior to surgery. no chemotherapy was ad ... | 1977 | 608548 |
| mouse tumour tests for quality control of c. parvum preparations. | description of two mouse tumor tests selected to control the quality of c. parvum pilot productions: transplanted ehrlich's ascitic tumor in inbred swiss mice and transplanted yc8 ascitic tumor in isogenic balb/c mice. the results obtained during three years of control are analyzed, particularly accuracy, long range adequacy and reproducibility of tumor challenges and c, parvum stimulations. the effects of some parameters of the tumor tests, e.g. dose-responses, treatment schedules, tumor-host r ... | 1977 | 608550 |
| c. parvum skin testing antigen: study on guinea pig model. | a skin-testing antigen produced from c. parvum has been developed for exploring cell-mediated immunity and specially c. parvum specific cell-mediated immunity by delayed cutaneous hypersensitivity (dch) reaction. the dch antigen and the techniques of intradermal injection and multiple puncture are described. dch reactions are carried out in c. parvum specifically sensitized guinea pigs: sensitization procedure and adjuvant (ifa and cfa) effects are reported. measures of dch reactions for differe ... | 1977 | 608551 |
| proposals for quality control methods of bacterial vaccines for immunostimulation. i. general considerations. | control methods for this type of vaccines include tests on identity, absence of contaminating microorganisms and safety which are required for any vaccine. in addition, for the control of vaccines for immunostimulation in cancer therapy some special methods for safety and potency testing are required. some assays are proposed, such as stimulation of lymphocytes in vivo and in vitro, stimulation of macrophages (listeria clearance) in mice and the prophylactic and therapeutic tumor model in mice. ... | 1977 | 608552 |
| the effect of intradermal administration of corynebacterium parvum on the immune response to hepatitis bs antigen. | the immunopotentiating effect of the intradermal administration of a course of four doses (0.25 ml) of a standard suspension of killed c. parvum (2 mg/ml) was studied in a group of 10 asymptomatic chronic hepatitis b surface antigen (hbsag) carriers, as well as in 11 persons with antibodies to hbsag (anti-hbs) and six without hbsag or anti-hbs. hbsag, anti-hbs, and leukocyte migration inhibition (lmi) studies were performed in pre- and post-inoculation blood samples. c. parvum produced a substan ... | 1977 | 609027 |
| measurement of phagocytic activity of reticuloendothelial system (res) by intralipid: effect of c parvum treatment. | | 1977 | 617547 |
| [study of the tolerance of a submucous injection of coryné parvum in the dog bladder]. | | 1977 | 617754 |
| organ distribution of technetium-99m-labeled corynebacterium parvum in normal and tumor-bearing mice. | | 1978 | 618580 |
| correlation of antitumor chemoimmunotherapy with serum inhibition of tumor cell destruction. | the administration of cyclophosphamide and corynebacterium parvum in combination results in tumor growth inhibition greater than that resulting from the use of either agent alone. the precise mechanism(s) by which this chemoimmunotherapy combination results in a synergistic inhibiting effect is not known. the possibility was entertained that the tumor effect might be related to a greater decrease in serum-mediated interference with cellular cytotoxicity, i.e., "blocking" activity, by both agents ... | 1978 | 618581 |
| influence of immunotherapeutic agents on the progression of spontaneously arising, metastasizing rat mammary adenocarcinomas of varying immunogenicities. | | 1978 | 618584 |