| microflora of feta cheese from four greek manufacturers. | the components of the microflora of four feta cheeses, produced by different greek manufacturers, were determined by culture dependent and independent techniques. isolates from microbiological media were first grouped by polymerase chain reaction-denaturing gradient gel electrophoresis (pcr-dgge) and then representatives of each dgge group were sequenced for identification purposes. dna and rna, extracted directly from the cheese, were subjected to pcr-dgge. moreover, feta cheeses were subjected ... | 2008 | 18555549 |
| grana padano cheese whey starters: microbial composition and strain distribution. | the aim of this work was to evaluate the species composition and the genotypic strain heterogeneity of dominant lactic acid bacteria (lab) isolated from whey starter cultures used to manufacture grana padano cheese. twenty-four grana padano cheese whey starters collected from dairies located over a wide geographic production area in the north of italy were analyzed. total thermophilic lab streptococci and lactobacilli were quantified by agar plate counting. population structure of the dominant a ... | 2008 | 18620769 |
| bacteriocin-producing lactobacillus strains isolated from poto poto, a congolese fermented maize product, and genetic fingerprinting of their plantaricin operons. | thirty one bacteriocin-producing lactobacillus isolates were identified among 135 lactobacilli isolated from the congolese fermented maize product poto poto, during the preparation and from the finished product. using species-specific pcr and 16s rrna gene sequencing, 28 and 3 isolates were identified as l. plantarum and l. fermentum, respectively. cluster analysis of rapd-pcr fingerprints revealed two main groups (g1 and g2) plus the l. fermentum isolate c4-13. group g1 contained 23 isolates wi ... | 2008 | 18620772 |
| in vitro effects of selected synbiotics on the human faecal microbiota composition. | synbiotics are recognized means of modulating gut microbiota composition and activities. however, whether synbiotics are superior to prebiotics and probiotics alone in moderating the gut microbiota towards a purportedly healthy composition has not been determined. eight selected synbiotics (short-chain fructooligosaccharides or fructooligosaccharides, each combined with one of four probiotics, lactobacillus fermentum me-3, lactobacillus plantarum wcfs1, lactobacillus paracasei 8700:2 or bifidoba ... | 2008 | 18673391 |
| eradication of salmonella typhimurium infection in a murine model of typhoid fever with the combination of probiotic lactobacillus fermentum me-3 and ofloxacin. | the aim of the study was to detect whether in experimental salmonella enterica typhimurium infection the probiotic lactobacillus fermentum me-3 in combination with fluoroquinolone therapy would eradicate s. typhimurium, prevent the development of liver and spleen granulomas and improve the indices of oxidative stress in the ileum mucosa. the selected bacteriological, histological and biochemical methods were applied. | 2008 | 18680567 |
| selection and use of autochthonous mixed starter for lactic acid fermentation of carrots, french beans or marrows. | strains of leuconostoc mesenteroides, lactobacillus plantarum, weissella soli/weissella koreensis, enterococcus faecalis, pediococcus pentosaceus and lactobacillus fermentum were identified from raw carrots, french beans and marrows by partial 16s rrna gene sequence. l. plantarum m1, leuc. mesenteroides c1 and p. pentosaceus f4 were selected based on the rates of growth and acidification in vegetable juice media, and used as the autochthonous mixed starter for the fermentation of carrots, french ... | 2008 | 18710789 |
| a traditional sudanese fermented camel's milk product, gariss, as a habitat of streptococcus infantarius subsp. infantarius. | samples of the traditional sudanese fermented camel's milk product gariss representing 9 different regions in sudan were microbiologically characterized using an integrated approach including phenotypic and genotypic methods. lactic acid bacteria [log(cfu/g)=7.76-8.66] and yeasts [log(cfu/g)=6.05-7.79] were detected in high numbers. a total of 180 lab isolates were identified of which 123 isolates were found to belong to the streptococcus bovis group. thirteen representative isolates were charac ... | 2008 | 18774196 |
| expanding substrate specificity of nucleoside 2'-deoxyribosyltransferase. | nucleoside 2'-deoxyribosyltransferase (ndt) is used to synthesize unnatural 2'-deoxyribonucleosides, modified mostly on the heterocyclic base. here we describe a strategy for improving 2,3-dideoxyribosyl(ddr) transfer activity of ndt by combining mutagenesis and in vivo selection in e. coli. | 2008 | 18776470 |
| kinetic analysis of growth and sugar consumption by lactobacillus fermentum imdo 130101 reveals adaptation to the acidic sourdough ecosystem. | the effect of ph on growth and carbohydrate metabolism of l. fermentum imdo 130101 was investigated. pronounced acid tolerance occurred together with marked responses in sugar metabolism due to acid stress. in accordance with the environment from which this strain was isolated, glucose and fructose metabolism remained active at low ph. fructose was quantitatively converted into mannitol under all conditions tested, yielding an energetic advantage to the strain. modelling of growth, sugar consump ... | 2008 | 18783841 |
| isolation and characterization of potential probiotic lactobacilli from pig feces. | this study examined four lactobacilli isolated from pig feces. two hundred lactic acid bacteria strains were obtained from pig feces using selective culture media (with vancomycin and bromocresol green; termed lamvab agar). microscopy, the catalase test, gram-staining, and rapd-pcr analysis were used to group the bacteria into 20 related clusters. phenotypic analysis using the api 50 ch test and genotypic analysis of 16s rdna sequences identified these clusters as representing single strains of ... | 2009 | 18792047 |
| effects of initial air removal methods on microorganisms and characteristics of fermented plant beverages. | the effects of 3 different methods for removing the initial air on the properties of fermented plant beverages produced from phom-nang seaweed (gracilaria fisheri) and wild forest noni (morinda coreia ham.) were investigated. only method m which covered the space above the fermentation liquid with a water filled plastic bag produced no surface film of yeast, had the highest acidity and also antibacterial activity from both plants after 90 days of fermentation. however, the yeast count still exce ... | 2008 | 18817186 |
| the effect of long-term lactobacilli (lactic acid bacteria) enteral treatment on the central nervous system of growing rats. | the aim of this study was to explore the relationship between consumption of large doses of lactic acid bacteria (lab) and the behaviour and brain morphobiochemistry of normal growing rats. four groups of rats were treated with lab cultures twice daily for 6 months. the control group received 1 ml of saline per treatment, while two experimental groups received 1 ml of living bacteria (lactobacillus plantarum and lactobacillus fermentum, respectively) and the remaining group received a heat-treat ... | 2009 | 18829284 |
| use of lactobacillus strains to start cassava fermentations for gari production. | two lactobacillus strains, lactobacillus plantarum bfe 6710 and lactobacillus fermentum bfe 6620, were used to start cassava fermentations in a pilot study under field production conditions in kenya, to determine their potential to establish themselves as predominant lactobacilli during the fermentation. predominant strains from three fermentations were isolated throughout the 48 h fermentation period. the use of these strains in high numbers clearly resulted in 1 to 2 log higher lactic acid bac ... | 2008 | 18834642 |
| enzyme activities of lactic acid bacteria from a pearl millet fermented gruel (ben-saalga) of functional interest in nutrition. | lactic acid bacteria responsible for the fermentation of a pearl-millet based fermented gruel, ben-saalga, were investigated for enzyme activity in relation with the nutritional characteristics of gruels used as complementary foods for young children. thirty pre-selected lab from a set of 155 isolates were characterized principally for their ability to produce amylase, phytase and alpha-galactosidase. two lactobacillus plantarum strains (4.4 and 6.1) and three lactobacillus fermentum strains (11 ... | 2008 | 18937991 |
| rep-pcr characterization and biochemical selection of lactic acid bacteria isolated from the delta area of egypt. | samples of raw milk and traditional dairy products were collected from different rural areas in the delta region. 170 isolates from these products were identified using repetitive genomic element-pcr (rep-pcr) fingerprinting. the identified isolates were tested for efficiency of biomass production and separation, acidifying activity, autolytic and aminopeptidase properties, antagonistic activities and exopolysaccharide production. the obtained results revealed that the lactobacillus delbrueckii ... | 2009 | 18992953 |
| small-intestinal manifestations of dextran sulfate sodium consumption in rats and assessment of the effects of lactobacillus fermentum br11. | the dextran sulfate sodium (dss) colitis model has been utilized to screen for novel therapeutics for ulcerative colitis. evidence suggests the small intestine may also be affected by dss. we characterized the effects of dss on the small intestine and assessed the potential for lactobacillus fermentum br11 to modify or normalize dss-induced changes. rats were allocated to three groups, water + vehicle, dss + vehicle, and dss + l. fermentum br11. br11 was administered twice daily for 14 days. dss ... | 2009 | 19005763 |
| lactobacillus fermentum br11 and fructo-oligosaccharide partially reduce jejunal inflammation in a model of intestinal mucositis in rats. | although probiotics are beginning to enter mainstream medicine for disorders of the colon, their effects on the small bowel remain largely unexplored. we investigated the recently identified probiotic, lactobacillus fermentum (l. fermentum) br11 (br11) and the prebiotic, fructo-oligosaccharide (fos), both individually and in synbiotic combination, for their potential to alleviate intestinal mucositis. from days 0-9, rats consumed skim milk (sm; saline + sm), low dose (ld-br11; 1 x 10(6)cfu/ml), ... | 2008 | 19005975 |
| development of tablets containing probiotics: effects of formulation and processing parameters on bacterial viability. | the probiotic products available in the market nowadays are mostly in the form of liquid or semisolid formulations which show low cell viability after oral administration, mainly because the bacteria do not survive the harsh conditions in the stomach. the development of suitable dry dosage forms enable higher bacterial survival and consequently is the main aim of the present study. an anticipated advantage is that due to the low water-activity lyophilized bacterial cells will preserve their viab ... | 2009 | 19059323 |
| probiotic potential of lactic acid bacteria isolated from fermented dairy milks on antiproliferation of colon cancer cells. | fifty-four strains of lactic acid bacteria obtained from fermented dairy milks were investigated for possible use as probiotics and for colon cancer biological products. five of these strains inhibited growth of eight food-borne pathogens including helicobacter pylori, escherichia coli, and salmonella typhimurium. three of these strains survived at ph 2.5 and in 0.3% bile salts. additionally they produced no haemolysis, were resistant to kanamycin and adhered to caco-2 cells. 16s rrna gene seque ... | 2009 | 19116692 |
| identification and characterization of the two-component cell lysis cassette encoded by temperate bacteriophage phipyb5 of lactobacillus fermentum. | to characterize the two-component cell lysis cassette comprised of holin (hyb5) and endolysin (lyb5) encoded by lactobacillus fermentum temperate bacteriophage phipyb5, and illustrate the potential application of lyb5 as therapeutic agents. | 2008 | 19120640 |
| intestinal microbiota differentially affect brush border enzyme activity and gene expression in the neonatal gnotobiotic pig. | to study microbial influence on intestinal development pertaining to nutrient digestion, two separate gnotobiotic experiments were performed, each with 16 piglets allocated to four treatment groups: germfree (gf), monoassociation with escherichia coli, monoassociation with lactobacillus fermentum or conventionalization with faecal bacteria (cv). enzyme activity and gene expression of lactase phlorizin hydrolase (lph) and aminopeptidase n (apn) were measured in isolated enterocytes, harvested on ... | 2009 | 19141103 |
| modeling bacterial contamination of fuel ethanol fermentation. | the emergence of antibiotic-resistant bacteria may limit the effectiveness of antibiotics to treat bacterial contamination in fuel ethanol plants, and therefore, new antibacterial intervention methods and tools to test their application are needed. using shake-flask cultures of saccharomyces cerevisiae grown on saccharified corn mash and strains of lactic acid bacteria isolated from a dry-grind ethanol facility, a simple model to simulate bacterial contamination and infection was developed. chal ... | 2009 | 19148876 |
| a qualified presumption of safety approach for the safety assessment of grana padano whey starters. | a qualified presumption of safety (qps) approach was applied to dominant lactic acid bacteria (lab) associated with grana padano cheese whey starters. thirty-two strains belonging to lactobacillus helveticus, lactobacillus delbrueckii subsp. lactis, streptococcus thermophilus, and lactobacillus fermentum, and representing the overall genotypic lab diversity associated with 24 previously collected whey starters [rossetti, l., fornasari, m.e., gatti, m., lazzi, c., neviani, e., giraffa, g., 2008. ... | 2009 | 19187995 |
| aflatoxin b1 binding capacity of autochthonous strains of lactic acid bacteria. | some foods are prone to contamination with aflatoxins, with detrimental effect on human health. lactic acid bacteria have been reported to bind aflatoxins and remove them from foods and feeds. reduction of aflatoxin b1 (afb1) from the liquid media by the autochthonous lactic acid bacteria (lactobacillus casei, lactobacillus plantarum, and lactobacillus fermentum) isolated from traditional iranian sourdough and dairy products is reported in the current study. the effect of incubation time on the ... | 2009 | 19205485 |
| molecular characterization and expression analysis of the glucansucrase dsrwc from weissella cibaria synthesizing a alpha(1-->6) glucan. | weissella cibaria isolated from human saliva produces a soluble glucan that predominantly has alpha-1,6-glucosidic type linkages. using degenerated primers that were selected based on the amino acid sequences of conserved regions from known glucansucrases, a single 2.7-kb fragment was isolated. in subsequent steps, a 4969-bp product was obtained using inverse pcr. the coding region for the glucansucrase gene (dsrwc) consisted of a 4419-bp orf that encoded a 1472-amino acid protein with a calcula ... | 2009 | 19222580 |
| adaptability of lactic acid bacteria and yeasts to sourdoughs prepared from cereals, pseudocereals and cassava and use of competitive strains as starters. | the adaptability of lactic acid bacteria (lab) and yeasts to sourdoughs prepared from cereals, pseudocereals and cassava was investigated using pcr-dgge and bacteriological culture combined with rrna gene sequence analysis. sourdoughs were prepared either from flours of the cereals wheat, rye, oat, barley, rice, maize, and millet, or from the pseudocereals amaranth, quinoa, and buckwheat, or from cassava, using a starter consisting of various species of lab and yeasts. doughs were propagated unt ... | 2009 | 19239979 |
| functional properties of novel protective lactic acid bacteria and application in raw chicken meat against listeria monocytogenes and salmonella enteritidis. | in this study 635 lactic acid bacteria of food origin were evaluated for their potential application as protective cultures in foods. a stepwise selection method was used to obtain the most appropriate strains for application as protective cultures in chicken meat. specifically, all strains were examined for antimicrobial activity against various gram positive and gram negative pathogenic and spoilage bacteria. strains exhibiting anti-bacterial activity were subsequently examined for survival in ... | 2009 | 19249112 |
| phenotypic and molecular identification of lactobacilli isolated from vaginal secretions. | twenty lactobacilli isolated from vaginal samples of healthy volunteers were characterized according to polyphasic taxonomy. a broad spectrum of activity and protective properties of these vaginal isolates has been reported. | 2008 | 19255690 |
| lactobacillus fermentum cect 5716 prevents and reverts intestinal damage on tnbs-induced colitis in mice. | probiotics attenuate gut inflammation when administered before experimental colitis, but data on their effect after colitis induction are scarce. we aimed to evaluate the effects of lactobacillus fermentum cect 5716 on gut injury when administered either before or after trinitrobencene sulfonic acid (tnbs) colitis in balb/c mice. | 2009 | 19266568 |
| safety assessment of lactobacillus fermentum cect5716, a probiotic strain isolated from human milk. | lactobacillus fermentum cect5716, a probiotic strain isolated from human milk, was characterized in a previous study. the objective of this study was to evaluate its sensitivity to antibiotics and its potential toxicity and translocation ability after oral administration to mice. for this purpose, 40 balb/c mice were divided in two groups (n=20 per group). one group was treated orally with 10(10) colony forming units (cfu)/mouse/day of lb. fermentum cect5716 during 28 d. the other group only rec ... | 2009 | 19281631 |
| lactobacillus spp. associated with early childhood caries. | a group of 69 lactobacilli was isolated from caries lesions and root canals of early childhood caries (ecc) affected children treated in the department of pedodontics (children's teaching hospital, brno, czech republic). biochemical and physiological properties of all strains were characterized by api 50 ch kit and conventional tube tests. the rep-pcr fingerprinting with the (gtg)(5) primer was used for genotypic grouping of the isolates. the (gtg)(5)-pcr fingerprinting grouped all analyzed stra ... | 2009 | 19330545 |
| bacillus d-stereospecific metallo-amidohydrolase: active-site metal-ion substitution changes substrate specificity. | from investigation of 2000 soil isolates, we identified a d-stereospecific metallo-amidohydrolase that can hydrolyze d-aminoacyl derivatives from the culture supernatant of bacillus sp. 62e11: 62e11dppa. the enzyme binds two equivalents of zinc, exhibits 70% identity with that of d-aminopeptidases from bacillus subtilis (dppa). in fact, 62e11dppa has strict specificity toward d-aminoacyl derivatives, i.e., the enzyme shows high activity toward d-aminoacyl benzyl esters and little activity toward ... | 2009 | 19340926 |
| effects of a synbiotic product on blood antioxidative activity in subjects colonized with helicobacter pylori. | to evaluate the impact of the consumption of a synbiotic product on the antioxidative activity markers of blood in asymptomatic h. pylori-colonized persons. | 2009 | 19344357 |
| influence of lactobacillus fermentum i5007 on the intestinal and systemic immune responses of healthy and e. coli challenged piglets. | the effect of feeding lactobacillus fermentum i5007 on the immune system of weaned pigs with or without e. coli challenge was determined. twenty-four weaned barrows (6.07 +/- 0.63 kg bw) were randomly assigned to one of four treatments (n = 6) in a factorial design experiment. the first two treatments consisted of healthy piglets with half of the pigs receiving no treatment while the other half was orally administered with l. fermentum i5007 (10(8) cfu/ml) at a daily dose of 20 ml. pigs in the s ... | 2009 | 19347600 |
| different fecal microbiotas and volatile organic compounds in treated and untreated children with celiac disease. | this study aimed at investigating the fecal microbiotas of children with celiac disease (cd) before (u-cd) and after (t-cd) they were fed a gluten-free diet and of healthy children (hc). brothers or sisters of t-cd were enrolled as hc. each group consisted of seven children. pcr-denaturing gradient gel electrophoresis (dgge) analysis with v3 universal primers revealed a unique profile for each fecal sample. pcr-dgge analysis with group- or genus-specific 16s rrna gene primers showed that the lac ... | 2009 | 19376912 |
| lactobacillus fermentum me-3 - an antimicrobial and antioxidative probiotic. | the paper lays out the short scientific history and characteristics of the new probiotic lactobacillus fermentum strain me-3 dsm-14241, elaborated according to the regulations of who/fao (2002). l. fermentum me-3 is a unique strain of lactobacillus species, having at the same time the antimicrobial and physiologically effective antioxidative properties and expressing health-promoting characteristics if consumed. tartu university has patented this strain in estonia (priority june 2001, patent in ... | 2009 | 19381356 |
| rapid detection of lactobacillus and yeast concentrations using a particle size distribution analyser. | to see the possibility of particle size distribution analyser (psda) in detecting concentration of lactobacillus contaminants in yeast fermentation. | 2009 | 19426260 |
| identification and evaluation of the probiotic potential of lactobacilli isolated from canine milk. | canine milk protects puppies against infectious diseases through a variety of mechanisms. in this study, the presence of potentially probiotic lactic acid bacteria grown on mrs-cys agar plates from milk of nine bitches was investigated. the gram positive catalase negative bacilli identified in this study belonged to four lactobacillus species (lactobacillus reuteri, lactobacillus fermentum, lactobacillus murinus, lactobacillus animalis), as well as one isolate that was identified as weissella vi ... | 2010 | 19451012 |
| [influence of lactobacillus fermentum metabolites on ultrastructure of pathogenic escherichia coli]. | to study ultrastructural changes of hemolytic and enterohemorrhagic escherichia coli during interaction with metabolites of lactobacillus fermentum. | 2009 | 19459468 |
| orally delivered microencapsulated live probiotic formulation lowers serum lipids in hypercholesterolemic hamsters. | elevated serum cholesterol is a major risk factor for coronary artery disease. nutritional therapies such as probiotics have been suggested to manage elevated cholesterol. this study investigates the cholesterol and triglyceride lowering potential of a microencapsulated feruloyl esterase-producing lactobacillus fermentum 11976 (lf11976) probiotic formulation. male bio f(1)b hamsters were assigned to two groups to receive either the microcapsule probiotic formulation (containing lf11976 cells at ... | 2009 | 19459731 |
| free radical scavenging activity of lactobacillus fermentum in vitro and its antioxidative effect on growing-finishing pigs. | to evaluate the free radical-scavenging capacity of lactobacillus fermentum and its effects on antioxidant enzyme levels in finishing pigs. | 2009 | 19486423 |
| lactic acid bacteria as reducing and capping agent for the fast and efficient production of silver nanoparticles. | there is a growing demand for silver-based biocides, including both ionic silver forms and metallic nanosilver. the use of metallic nanosilver, typically chemically produced, faces challenges including particle agglomeration, high costs, and upscaling difficulties . additionally, there exists a need for the development of a more eco-friendly production of nanosilver. in this study, gram-positive and gram-negative bacteria were utilized in the non-enzymatic production of silver nanoparticles via ... | 2009 | 19488750 |
| the microbiota of lafun, an african traditional cassava food product. | lafun is a fermented cassava food product consumed in parts of west africa. in the present work the microorganisms (aerobic bacteria (ab), lactic acid bacteria (lab) and yeasts) associated with the fermentation of lafun under traditional conditions have for the first time been studied using a combination of pheno- and genotypic methods. during lafun fermentation the ab count ranged from 6-7 log(10) cfu/g at the beginning to 9 log(10) cfu/g at the end. similarly, the number of lab increased from ... | 2009 | 19493582 |
| a new strategy to apply bacillus subtilis ma139 for the production of solid-state fermentation feed. | the study investigated the potential of using bacillus subtilis ma139 in combination with lactobacillus fermentum and saccharomyces cerevisae to produce solid-state fermentation feed. | 2009 | 19500243 |
| lactobacillus fermentum bcs87 expresses mucus- and mucin-binding proteins on the cell surface. | to identify and characterize adhesion-associated proteins in the potential probiotic lactobacillus fermentum bcs87. | 2009 | 19548890 |
| culture-based pcr analysis of plaque samples of japanese school children to assess the presence of six common cariogenic bacteria and its association with caries risk. | the aim of the study was to assess the presence of six common cariogenic bacteria from cariostat-inoculated plaque samples of japanese elementary school children through pcr analysis and check its associations with caries risk testing the validity of cariostat as a caries risk assessment tool. this epidemiological school-based study investigated plaque samples of 399 japanese elementary school children. assessed using the cariostat, 48.2% of the children had high caries risk. dna detection of st ... | 2009 | 19573592 |
| bacillus d-stereospecific metallo-amidohydrolase: active-site metal-ion substitution changes substrate specificity. | from investigation of 2000 soil isolates, we identified a d-stereospecific metallo-amidohydrolase that can hydrolyze d-aminoacyl derivatives from the culture supernatant of bacillus sp. 62e11: 62e11dppa. the enzyme binds two equivalents of zinc, exhibits 70% identity with that of d-aminopeptidases from bacillus subtilis (dppa). in fact, 62e11dppa has strict specificity toward d-aminoacyl derivatives, i.e., the enzyme shows high activity toward d-aminoacyl benzyl esters and little activity toward ... | 2009 | 19602397 |
| isolation of cultivable thermophilic lactic acid bacteria from cheeses made with mesophilic starter and molecular comparison with dairy-related lactobacillus helveticus strains. | to isolate cultivable thermophilic lactic acid bacteria from cheeses made with mesophilic starter and compare them with dairy-related lactobacillus helveticus strains using molecular typing methods. | 2009 | 19627475 |
| oral lactobacillus species in type 2 diabetic patients living in southern thailand. | although lactobacilli are part of normal oral, gastrointestinal and genitourinary flora, they are an uncommon cause of infections in human. lactobacillus-associated infections have generally occurred in patients with serious underlying conditions e.g. diabetes and cancer that might favour certain microorganisms. the aim of this study was to characterize species and genotypes of lactobacilli isolated from diabetic patients and non-diabetic subjects. one hundred and five type 2 diabetic patients a ... | 2009 | 19655427 |
| identification of lactic acid bacteria isolated from tarhana, a traditional turkish fermented food. | tarhana is a traditional fermented product produced from a mixture of spontaneously fermented yogurt and wheat flour in turkey. the aims of the present study were to enumerate and identify for the first time by molecular biology-based methods predominant lactic acid bacteria (lab) isolated during processing of tarhana. samples were collected from eight different regions of turkey. in order to explore the relationship between raw material and the microbiology of tarhana, yogurt and wheat flour we ... | 2009 | 19703719 |
| determination of an economical medium for growth of lactobacillus fermentum using response surface methodology. | lactobacillus fermentum is a widely utilized probiotic compound fed as an alternative to antibiotics for growth promotion in a wide variety of livestock species. the objective of this research is to develop an economical and practical fermentation medium for the growth of lact. fermentum using response surface methodology. | 2009 | 19709366 |
| gastrointestinal microbial community shifts observed following oral administration of a lactobacillus fermentum strain to mice. | the indigenous gastrointestinal microbiota acts as an integral defense against the colonisation of orally introduced microbes. whilst this can be important in host protection, some introduced species, including lactobacilli, can have a positive impact on existing microbial communities. the interaction of a candidate probiotic strain of lactobacillus fermentum within the gastrointestinal tract was monitored in a mouse model and its effect on the indigenous microbiota observed. l. fermentum kld wa ... | 2003 | 19719673 |
| decolorization of water and oil-soluble azo dyes by lactobacillus acidophilus and lactobacillus fermentum. | the capability of lactobacillus acidophilus and lactobacillus fermentum to degrade azo dyes was investigated. the bacteria were incubated under anaerobic conditions in the presence of 6 microg/ml methyl red, ponceau bs, orange g, amaranth, orange ii, and direct blue 15; 5 microg/ml sudan i and ii; or 1.5 microg/ml sudan iii and iv in demann-rogosa-sharpe broth at 37 degrees c for 36 h, and reduction of the dyes was monitored. both bacteria were capable of degrading all of the water-soluble azo d ... | 2009 | 19727875 |
| environmental ph determines citrulline and ornithine release through the arginine deiminase pathway in lactobacillus fermentum imdo 130101. | sourdough lactic acid bacteria (lab) need to be adapted to a highly acidic and, therefore, challenging environment. different mechanisms are employed to enhance competitiveness, among which conversion of arginine into ornithine through the arginine deiminase (adi) pathway is an important one. a combined molecular and kinetic approach of the adi pathway in lactobacillus fermentum imdo 130101, a highly competitive sourdough lab strain, identified mechanisms with advantageous technological effects ... | 2009 | 19732985 |
| modulation of the immune response by probiotic strains in a mouse model of gluten sensitivity. | probiotic strains play an important role in modulating activities in the gut-associated lymphoid tissue. elucidation of the mechanisms that mediate probiotic-driven immunomodulation may facilitate their therapeutic application for specific immune-mediated diseases or for prophylaxis. in this study, we explored the effect of different lactobacillus spp. and bifidobacterium lactis in transgenic mice expressing the human dq8 heterodimer, a hla molecule linked to celiac disease (cd). in vitro analys ... | 2009 | 19736022 |
| the arginine deiminase pathway of lactobacillus fermentum imdo 130101 responds to growth under stress conditions of both temperature and salt. | the arginine deiminase (adi) pathway is a means by which certain sourdough lactic acid bacteria (lab) convert arginine into ornithine via citrulline while producing ammonia and atp, thereby coping with acid stress and gaining an energetic advantage. lactobacillus fermentum imdo 130101, an isolate from a spontaneous laboratory rye sourdough, possesses an adi pathway which is modulated by environmental ph. in the present study, a broader view of the activity of the adi pathway in response to growt ... | 2009 | 19747605 |
| screening of lactic acid bacteria from indonesia reveals glucansucrase and fructansucrase genes in two different weissella confusa strains from soya. | homopolysaccharide (glucan and fructan) synthesis from sucrose by sucrase enzymes in lactic acid bacteria (lab) has been well studied in the genera leuconostoc, streptococcus and lactobacillus. this study aimed to identify and characterize genes encoding glucansucrase/glucosyltransferase (gtf) and fructansucrases/fructosyltransferase (ftf) enzymes from genomic dna of 'rare' indonesian exopolysaccharide-producing lab. from a total of 63 exopolysaccharide-producing lab isolates obtained from foods ... | 2009 | 19758326 |
| antimicrobial activity, inhibition of urogenital pathogens, and synergistic interactions between lactobacillus strains. | lactobacillus fermentum strain l23 and l. rhamnosus strain l60 were selected as an alternative treatment to prevent or treat urogenital infections based on their probiotic properties and production of bacteriocins. the objectives of the present work were to study the inhibitory activities of these two bacteriocin-producing strains, and to analyze the interactions between pairs of bacteriocins that inhibit urogenital pathogens. antimicrobial activity tests of l23 and l60 were performed by a diffu ... | 2009 | 19777307 |
| vaginal colonization and activity of the probiotic bacterium lactobacillus fermentum l23 in a murine model of vaginal tract infection. | a strain of lactobacillus, identified as lactobacillus fermentum l23, was selected from among 100 strains isolated from vaginal swabs of healthy, non-pregnant, pre-menopausal women. l. fermentum l23 was chosen on the basis of its bacteriocinogenic ability and its properties relevant to colonization, i.e. self-aggregation, adherence to vaginal epithelial cells and co-aggregation with bacterial pathogens. the antimicrobial preventative and curative effects produced by the probiotic l. fermentum l2 ... | 2010 | 19926731 |
| characterization and expression analysis of the exopolysaccharide gene cluster in lactobacillus fermentum tds030603. | part of the exopolysaccharide gene cluster of lactobacillus fermentum tds030603 was characterized. it consists of 11,890 base pairs and is located in the chromosomal dna, 13 open reading frames of which were encoded. out of the 13 open reading frames, six were found to be involved in exopolysaccharide synthesis; however, five were similar to transposase genes of other lactobacilli, and two were functionally unrelated. expression analysis revealed that the exopolysaccharide synthesis-related gene ... | 2009 | 19966483 |
| biogenic silver for disinfection of water contaminated with viruses. | the presence of enteric viruses in drinking water is a potential health risk. growing interest has arisen in nanometals for water disinfection, in particular the use of silver-based nanotechnology. in this study, lactobacillus fermentum served as a reducing agent and bacterial carrier matrix for zerovalent silver nanoparticles, referred to as biogenic ag(0). the antiviral action of biogenic ag(0) was examined in water spiked with an enterobacter aerogenes-infecting bacteriophage (uz1). addition ... | 2010 | 20038697 |
| probiotic attributes of lactobacillus strains isolated from food and of human origin. | lactobacilli isolated from various sources were identified on the basis of 16s-23s rrna gene intergenic region amplification and subsequent sequencing of the smaller intergenic region. an in vitro analysis of probiotic properties including binding, ability to tolerate different concentrations of bile, survival in acidic buffer and antimicrobial activity of four different isolates and two standard strains (lactobacillus plantarum american type culture collection (atcc) 8014 and l. rhamnosus gg (l ... | 2010 | 20070917 |
| characterization of a cryptic plasmid pd403 from lactobacillus plantarum and construction of shuttle vectors based on its replicon. | a cryptic plasmid pd403 was isolated from lactobacillus plantarum d403 derived from fermented dairy products. it was 2,791 bp in size with a g+c content of 37%. nucleotide sequence analysis revealed two open reading frames, orf1 and orf2. orf1 (318 amino acids) was identified as a replication protein (repa). orf2 (137 amino acids) shared 31% similarity with the transcriptional regulator of ralstonia pickettii 12d. functional investigation indicated that orf2 (tra) had the ability of improving th ... | 2010 | 20077035 |
| two stomach-originated lactobacillus strains improve helicobacter pylori infected murine gastritis. | to investigate the potential anti-helicobacter pylori (h. pylori) and anti-inflammation in vivo effects of two lactobacillus strains from human stomach. | 2010 | 20101769 |
| lactic acid bacteria efficiently protect human and animal intestinal epithelial and immune cells from enteric virus infection. | this study aimed to examine the potential antiviral activity of lactic acid bacteria (lab) using animal and human intestinal and macrophage cell line models of non tumor origin. to this end, lab strains selected on the basis of previous in vitro trials were co-incubated with cell line monolayers, which were subsequently challenged with rotavirus (rv) and transmissible gastroenteritis virus (tgev). in order to elucidate the possible mechanism responsible for the antiviral activity, the induction ... | 2010 | 20106541 |
| effect of stress pretreatment on survival of probiotic bacteria in gastrointestinal tract simulator. | the effect of stress pretreatment on survival of probiotic lactobacillus acidophilus la-5, lactobacillus rhamnosus gg, and lactobacillus fermentum me-3 cultures was investigated in the single bioreactor gastrointestinal tract simulator (gits). the cultures were pregrown in ph-auxostat, subjected to temperature, acid, or bile stress treatment, fast frozen in liquid nitrogen (ln(2)), and tested for survival in gits. after ln(2) freezing the colony forming ability of l. rhamnosus gg and l. fermentu ... | 2010 | 20107984 |
| [biodiversity of lactobacilli from fermented milk products in northern tibet]. | in order to study the biodiversity of lactobacilli from northern tibet in china, we developed enterobacterial repetitive intergenic consensus sequence polymerase chain reaction (eric-pcr) technique. | 2009 | 20112685 |
| lactobacillus species in supragingival plaque in subjects with hyposalivation. | the aim of the study was to analyse the frequency of different lactobacillus species in relation to the ph-lowering potential of the plaque. | 2010 | 20149344 |
| microbial risk markers for childhood caries in pediatricians' offices. | dental caries in pre-school children has significant public health and health disparity implications. to determine microbial risk markers for this infection, this study aimed to compare the microbiota of children with early childhood caries with that of caries-free children. plaque samples from incisors, molars, and the tongue from 195 children attending pediatricians' offices were assayed by 74 dna probes and by pcr to streptococcus mutans. caries-associated factors included visible plaque, chi ... | 2010 | 20164496 |
| identification of lactic acid bacteria associated with the production of plaa-som, a traditional fermented fish product of thailand. | plaa-som is a thai fermented fish product for which whole fish or fish fillets are fermented with either cooked rice or steamed sticky rice, salt, and garlic. a total of 762 lactic acid bacteria (lab) were isolated during plaa-som fermentation by culture on caco(3)-mrs agar plates. they were screened and grouped by amplified ribosomal dna restriction analysis (ardra), giving six groups that were identified by ribosomal dna sequencing as lactococcus garvieae, streptococcus bovis, weissella cibari ... | 2010 | 20167386 |
| characterization of a novel lysm domain from lactobacillus fermentum bacteriophage endolysin and its use as an anchor to display heterologous proteins on the surfaces of lactic acid bacteria. | the endolysin lyb5, from lactobacillus fermentum temperate bacteriophage phipyb5, showed a broad lytic spectrum against gram-positive as well as gram-negative bacteria. sequence analysis revealed that the c terminus of the endolysin lyb5 (ly5c) contained three putative lysin motif (lysm) repeat regions, implying that ly5c was involved in bacterial cell wall binding. to investigate the potential of ly5c for surface display, green fluorescent protein (gfp) was fused to ly5c at its n or c terminus ... | 2010 | 20173067 |
| in vitro immunomodulatory activity of lactobacillus fermentum cect5716 and lactobacillus salivarius cect5713: two probiotic strains isolated from human breast milk. | commensal bacteria, including some species of lactobacilli commonly present in human breast milk, appear to colonize the neonatal gut and contribute to protection against infant infections, suggesting that lactobacilli could potentially modulate immunity. in this study, we evaluated the potential of two lactobacillus strains isolated from human milk to modulate the activation and cytokine profile of peripheral blood mononuclear cell (pbmc) subsets in vitro. moreover, these effects were compared ... | 2010 | 20219262 |
| intestinal lactobacillus sp. is associated with some cellular and metabolic characteristics of blood in elderly people. | the higher counts or particular groups (firmicutes/bacteroidetes) of intestinal microbiota are related to host metabolic reactions, supporting a balance of human ecosystem. we further explored whether intestinal lactobacilli were associated with some principal cellular and metabolic markers of blood in 38 healthy >65-year-old persons. the questionnaire, routine clinical and laboratory data of blood indices as much as the oxidized low-density lipoprotein (ox-ldl) and baseline diene conjugates in ... | 2010 | 20223288 |
| modifications in the lactobacillus fermenti 36 assay for thiamine. | | 1946 | 20276194 |
| a novel nitric oxide producing probiotic patch and its antimicrobial efficacy: preparation and in vitro analysis. | microbial and fungal infections are a significant consideration in the etiology of all wounds. numerous antimicrobial and antifungal formulations have been developed with varying degrees of efficacy and stability. here, we report a nitric oxide producing probiotic adhesive patch device and investigate its antimicrobial and antifungal efficacy in vitro. this probiotic patch utilizes the metabolic activity of immobilized lactic acid bacteria, glucose, and nitrite salts for the production of gaseou ... | 2010 | 20300748 |
| lactobacillus species and genotypes associated with dental caries in thai preschool children. | lactobacilli have been associated with the presence and progression of dental caries. nevertheless, the relation between certain species or genotypes of lactobacillus and caries is unclear and there are no data available for the thai population. this study aimed to examine the distribution of species and genotypes of oral lactobacillus among children with rather high caries prevalence, and to investigate whether certain species or genotypes were more related to caries activity than others. one h ... | 2010 | 20331803 |
| effect of inoculated corn silage enriched with sunflower oil on rumen fermentation and lipid metabolism in an artificial rumen (rusitec). | some rumen isolates are able to produce conjugated linoleic acid (cla) from linoleic acid (la) in vitro. effects of providing diets containing corn silage (cs) and lucerne hay to an artificial rumen (rusitec) in which the corn was not inoculated (cs), or inoculated with lactobacillus plantarum ccm 4000 (cs + lp), lactobacillus fermentum lf2 (cs + lf) or enterococcus faecium ccm 4231 (cs + ef) and supplied with sunflower oil (so; 30 g kg(-1); w/w) on rumen metabolism were examined. | 2010 | 20355015 |
| comparative evaluation of automated ribotyping and rapd-pcr for typing of lactobacillus spp. occurring in dental caries. | a group of 67 lactobacillus spp. strains containing lactobacillus casei/paracasei, lactobacillus fermentum, lactobacillus gasseri, lactobacillus plantarum, lactobacillus rhamnosus and lactobacillus salivarius species isolated from early childhood caries and identified to the species level in a previous study (svec et al., folia microbiol 54:53-58, 2009) was characterized by automated ribotyping performed by the riboprinter microbial characterization system and by randomly amplified polymorphic d ... | 2010 | 20361257 |
| identification of a new adhesin-like protein from lactobacillus mucosae me-340 with specific affinity to the human blood group a and b antigens. | to identify and characterize a new adhesin-like protein of probiotics that show specific adhesion to human blood group a and b antigens. | 2010 | 20408914 |
| identification of the bacterial biodiversity in koumiss by denaturing gradient gel electrophoresis and species-specific polymerase chain reaction. | bacterial biodiversity in traditional koumiss fermented milk was studied by denaturing gradient gel electrophoresis (dgge). target dna bands were identified according to the reference species ladder, constructed in this study. comigrating bands present in the dgge profiles were resolved by species-specific pcr. the results revealed a novel bacterial profile and extensive bacterial biodiversity in koumiss. the dominant lactic acid bacteria included lactobacillus acidophilus, lactobacillus helveti ... | 2010 | 20412906 |
| safety and functional aspects of pre-selected lactobacilli for probiotic use in iberian dry-fermented sausages. | the purpose of this study was to investigate lactobacilli for potential probiotic use in iberian dry-fermented sausages. a total of 18 strains, previously selected, isolated from different sources were evaluated according to safety and functional characteristics including biogenic amines and d-lactic acid production, antibiotic susceptibility, haemolysis, cell adhesion, and antimicrobial activity against food-borne pathogens. the strains lactobacillus reuteri pl519, l. reuteri pl542, and lactoba ... | 2009 | 20416680 |
| [k+/na+ in colonies of lactic bacteria lactobacillus fermentum 94c]. | | 2010 | 20432712 |
| urea degradation in some white wines by immobilized acid urease in a stirred bioreactor. | a purified acid urease preparation was covalently immobilized onto either eupergit c 250 l or glutaraldehyde-cross-linked chitosan-derivative beads (i.e., chitopearls bcw-3003 and bcw-3010). the kinetics of urea degradation in two target italian white (i.e., grechetto and sauvignon blanc) wines, as well as in a model wine solution, by using the above eupergit c 250 l-, bcw-3003-, or bcw-3010-based biocatalysts, was confirmed to be of the pseudofirst order with respect to the urea concentration i ... | 2010 | 20446734 |
| antimicrobial activity of phenolic acids against commensal, probiotic and pathogenic bacteria. | phenolic acids (benzoic, phenylacetic and phenylpropionic acids) are the most abundant phenolic structures found in fecal water. as an approach towards the exploration of their action in the gut, this paper reports the antimicrobial activity of thirteen phenolic acids towards escherichia coli, lactobacillus spp., staphylococcus aureus, pseudomonas aeruginosa and candida albicans. the growth of e. coli atcc 25922 was inhibited by only four of the phenolic acids tested at a concentration of 1000 m ... | 2010 | 20451604 |
| treatment of infectious mastitis during lactation: antibiotics versus oral administration of lactobacilli isolated from breast milk. | background: mastitis is a common infectious disease during lactation, and the main etiological agents are staphylococci, streptococci, and/or corynebacteria. the efficacy of oral administration of lactobacillus fermentum cect5716 or lactobacillus salivarius cect5713, two lactobacilli strains isolated from breast milk, to treat lactational mastitis was evaluated and was compared with the efficacy of antibiotic therapy. methods: in this study, 352 women with infectious mastitis were randomly assig ... | 2010 | 20455694 |
| lactic acid bacteria colonization and clinical outcome after probiotic supplementation in conventionally treated bacterial vaginosis and vulvovaginal candidiasis. | this randomized double-blind placebo controlled study assessed the vaginal colonization of lactic acid bacteria and clinical outcome. vaginal capsules containing l gasseri ln40, lactobacillus fermentum ln99, l. casei subsp. rhamnosus ln113 and p. acidilactici ln23, or placebos were administered for five days to 95 women after conventional treatment of bacterial vaginosis and/or vulvovaginal candidiasis. vulvovaginal examinations and vaginal samplings were performed before and after administratio ... | 2010 | 20472091 |
| bioconversion of isoflavone glycosides to aglycones, mineral bioavailability and vitamin b complex in fermented soymilk by probiotic bacteria and yeast. | to study the role of β-glucosidase producing probiotic bacteria and yeast in the biotransformation of isoflavone glycosides to aglycones, mineral bioavailability and vitamin b complex in fermented soymilk. | 2010 | 20477889 |
| functional characteristics of lactobacillus fermentum f1. | in this study, lactobacillus fermentum (l. fermentum) f1 reduced cholesterol 48.87%. the strain was screened from cattle feces using an api 50 chl system and the 16s rrna sequence contrasting method. l. fermentum f1 showed acid and bile tolerance, and antimicrobial activity against pathogenic escherichia coli and staphylococcus aureus. l. fermentum f1 deconjugated 0.186 mm of free cholalic acid after it was incubated at 37°c in 0.20% sodium taurocholate (tca) broth for 24 h. heat-killed and rest ... | 2011 | 20490496 |
| identification and characterization of a glucan-producing enzyme from lactobacillus hilgardii tmw 1.828 involved in granule formation of water kefir. | water kefir is a home made fermented beverage based on a sucrose solution with fruit extracts. the inoculum of such fermentations consists of macroscopic granula containing lactic and acetic acid bacteria, and yeasts, which are embedded in an exopolysaccharide (eps) matrix. in this work, a strain of lactobacillus hilgardii producing large amounts of the granule-forming dextran could be isolated. the glycosyltransferans (gtf) commonly called glucansucrase responsible for the production of this de ... | 2010 | 20510787 |
| development of starter culture for improved processing of lafun, an african fermented cassava food product. | to select appropriate micro-organisms to be used as starter culture for reliable and reproducible fermentation of lafun. | 2010 | 20553347 |
| purification of bacterial genomic dna in less than 20 min using chelex-100 microwave: examples from strains of lactic acid bacteria isolated from soil samples. | we established a chelex 100-microwave method for the purification of bacterial genomic dna (gdna) in less than 20 min with high yield and good quality, useful for multiple purposes. it combines chelex 100, proteinase k, rnase a and heating in a microwave oven. the resulting gdna was used directly to identify bacterial species of the order lactobacillales by means of pcr amplification of their 16s rdna gene, isolated from sediments on the yucatan peninsula, mexico. this method produced gdna free ... | 2010 | 20556655 |
| community dynamics of bacteria in sourdough fermentations as revealed by their metatranscriptome. | the lactic acid bacterial (lab) community dynamics of two wheat and two spelt sourdough fermentations that were daily back-slopped were monitored during a period of 10 days by hybridizing time-related rna samples, representing the metatranscriptome, to an lab functional gene microarray. to indicate the species present in each hybridized sample, annotation information for the 2,269 oligonucleotides on the microarray was used. the overall hybridization data revealed that after a transition phase o ... | 2010 | 20581179 |
| effect of lactobacillus fermentum and enterococcus faecium strains on internal milieu, antioxidant status and body weight of broiler chickens. | the aim of the present study was to evaluate the functional efficiency of two probiotic strains lactobacillus fermentum ccm 7158 and enterococcus faecium m 74 given to the drinking water on internal milieu, antioxidant status and body weight of broiler chickens. the experiment was conducted on hybrid hybro (n = 180). the feeding period lasted 42 days. experimental chickens of e1 group received a probiotic preparation in drinking water with concentration of 1 × 10(9) colony forming units (cfu) of ... | 2010 | 20626505 |
| diversity of lactic acid bacteria from hussuwa, a traditional african fermented sorghum food. | the diversity of lactic acid bacteria associated with hussuwa fermentation, a sudanese fermented sorghum food, was studied using a polyphasic taxonomical approach. predominant strains could be well characterised based on a combination of phenotypic tests and genotypic methods such as ardra, rep-pcr and rapd-pcr, as well as 16s rrna gene sequencing of representative strains. thus, the majority (128 of 220, 58.3%) of strains exhibited phenotypic properties typical of heterofermentative lactobacill ... | 2010 | 20630317 |
| complete genome sequence of lactobacillus fermentum cect 5716, a probiotic strain isolated from human milk. | lactobacillus fermentum is a heterofermentative lactic acid bacterium and is frequently isolated from mucosal surfaces of healthy humans. lactobacillus fermentum cect 5716 is a well-characterized probiotic strain isolated from human milk and, at present, is used in commercial infant formulas. here, we report the complete and annotated genome sequence of this strain. | 2010 | 20639335 |
| identification of lactic acid bacteria isolated from the fecal samples of healthy humans and patients with dyspepsia, and determination of their ph, bile, and antibiotic tolerance properties. | the research of novel probiotic strains is important in order to satisfy the increasing request of the market and to obtain functional products in which the probiotic cultures are more active and have better probiotic characteristics than those already on the market. in this study, the probiotic potential of lactobacillus and enterococcus strains isolated from human feces was investigated. one hundred seven strains of lactic acid bacteria were isolated from the fecal samples of 19 adult voluntee ... | 2010 | 20668388 |
| phenolic acids and flavonoids in nonfermented and fermented red sorghum ( sorghum bicolor (l.) moench). | this study aimed to identify phenolic acids and flavonoids in the red sorghum variety pan 3860 and to determine changes in their concentrations during fermentation with lactobacilli. sorghum sourdoughs fermented with two binary strain combinations, lactobacillus plantarum and lactobacillus casei or lactobacillus fermentum and lactobacillus reuteri , were compared to chemically acidified controls. four glycerol esters were tentatively identified, caffeoylglycerol, dicaffeoylglycerol, coumaroyl-ca ... | 2010 | 20677784 |
| nisin inhibits dental caries-associated microorganism in vitro. | nisin, produced by lactococcus lactis, is an antibiotic peptide to effectively antagonize a broad spectrum of gram-positive bacteria, and is widely used as a safe food antimicrobial agent. in the present study, we investigated whether nisin could be used as an effective antibiotic peptide against the nine common cariogenic microorganisms, and its antimicrobial activity could be affected by the ingredients of saliva in oral cavity. in the minimal inhibitory concentration (mic) and minimal bacteri ... | 2010 | 20688123 |
| glycemic index and phenolics of partially-baked frozen bread with sourdough. | different lactic acid bacteria starters were used to prepare sourdough to make partially-baked frozen wholemeal wheat bread. the sourdough was prepared with a pure culture of lactobacillus plantarum or with commercial starters containing lactobacillus brevis combined with saccharomyces cerevisiae var. chevalieri (lv4), lactobacillus fermentum (pl1), or lactobacillus fermentum with phytase (pl3). we determined the acetic and lactic acid concentrations in sourdough, bread chemical composition, tot ... | 2011 | 20715901 |
| characterization of dextran-producing weissella strains isolated from sourdoughs and evidence of constitutive dextransucrase expression. | the study of exopolysaccharide production by heterofermentative sourdough lactic acid bacteria has shown that weissella strains isolated from sourdoughs produce linear dextrans containing α-(1→6) glucose residues with few α-(1→3) linkages from sucrose. in this study, several dextran-producing strains, weissella cibaria and weissella confusa, isolated from sourdough, were characterized according to carbohydrate fermentation, repetitive element-pcr fingerprinting using (gtg)(5) primers and glucans ... | 2010 | 20722740 |