| vaccination of pregnant sows with clostridium perfringens type c toxoid. | | 1977 | 194385 |
| [early stages of the infectious process]. | | 1977 | 194438 |
| [effect of alcoholic intoxication on the body's natural resistance to exposure to infectious and toxic agents]. | the influence of alcoholic intoxication on the resistance of albino mice to bacterial toxins and staphylococcus cultures was investigated. five-day administration of 40% ethyl alcohol to the animals was accompanied by a significant increase of their resistance to the intoxication caused by c1. perfringens toxins and staphylococcus. thirty-day alcoholic intoxication promoted a marked reduction of albino mice resistance to the both toxins used and the staphylococcus cultures. | 1977 | 194439 |
| experience with a new agar culture medium for demonstrating the presence of anaerobic bacteria. | in view of the fact that anaerobic diagnostics have become a matter of primary importance, the authors have developed the rcm medium with certain modifications. owing to its good keeping quality, this medium can be used also on smaller laboratories for demonstrating the presence of anaerobic bacteria and for carrying out germ counts. it constitutes and adequate base for the anaerobic blood medium and for the wilson-blair medium, and also for the determination of the antibiotic resistance of the ... | 1975 | 194528 |
| comparison of sulfite-polymyxin-sulfadiazine medium and tryptose-sulfite-cycloserine medium without egg yolk for recovering clostridium perfringens. | the overall recoveries of spores and of actively growing, heat-stressed, coldshocked, and frozen cells of five strains of clostridium perfringens were significantly greater (95% confidence limits) on tryptose-sulfite-cycloserine medium without egg yolk than on sulfite-polymyxin-sulfadiazine medium. | 1977 | 194535 |
| the pathology of necrotic enteritis of chickens following infusion of broth cultures of clostridium perfringens into the duodenum. | necrotic enteritis was consistently reproduced when enough active broth culture of clostridium perfringens type a was infused intraduodenally. typical lesions of necrotic enteritis were seen as early as 5 hr after infusion was begun. the histologic lesions observed at 1 hr were characterized by edema in the lamina propria and desquamation of epithelial cells. large numbers of clostridia were seen among these sloughed cells. coagulation necrosis of the tips of villi became evident at 3 hr and was ... | 1977 | 194570 |
| the pathology of necrotic enteritis of chickens following infusion of crude toxins of clostridium perfringens into the duodenum. | necrotic enteritis was produced in 4-week-old chickens with bacteria-free crude toxins of clostridium perfringens. typical gross lesions could be seen as early as 3 hr after intraduodenal infusion of toxin was begun. early microscopic lesions were observed at 20 minutes, progressing with time to involve most of the mucosa. death was related to the amount of toxin administered. because systemic absorption of toxin is likely, acutely affected birds may die from toxemia. | 1977 | 194571 |
| the interaction of clostridium perfringens and its toxins in the production of necrotic enteritis of chickens. | the intraduodenal administration of large numbers of clostridium perfringens cells harvested from broth cultures and resuspended in pbs or fresh sterile thioglycollate broth produced a very mild form of necrotic enteritis. administering an appropriate number of cells in culture supernatant, however, produced typical field-type disease. alpha toxin was shown to be the significant toxin recoverable from broth-culture supernatant fluids. requirements to produce the disease are minor intestinal dama ... | 1977 | 194572 |
| [tween 80 influence on lecithinase c clostridium perfringens bp6k production (author's transl)]. | | 1977 | 194712 |
| the effect of phospholipase c, phospholipase a2 and neuraminidase on the uptake of [3h]norepinephrine and [3h]serotonin by rat brain synaptosomes. | | 1977 | 195016 |
| effect of protease on the production.of clostridium perfringens alpha toxin. | | 1977 | 195109 |
| equine intestinal clostridiosis. an acute disease in horses associated with high intestinal counts of clostridium perfringens type a. | | 1977 | 195449 |
| reproduction and treatment of necrotic enteritis in broilers. | a change in ration from one containing 50% fish meal to a standard chick starter containing 10(8) clostridium perfringens/g of feed resulted in the production of necrotic enteritis in 2-week-old broiler chicks. lincomycin added to the inoculated ration at a concentration of 20 g/907.2 kg significantly reduced mortality in the chicks. inoculation of broth cultures of c perfringens directly into the duodenum, using a surgically inserted tetrafluoroethylene resin tube, indicated a relationship exis ... | 1977 | 195498 |
| induction of bacteriocins from clostridium perfringens by treatment with mitomycin c. | six of ten bacteriocinogenic strains of clostridium perfringens were inducible by treating the cultures with mitomycin c. induction did not occur in the continued presence of mitomycin c but only when excess mitomycin c was removed. cell death was associated with induction of bacteriocin. | 1977 | 195516 |
| bacteriology of the human biliary tract and the duodenum. | using the modern anaerobic transport media and meticulous culture techniques, 74 patients undergoing biliary tract surgery were studied. the biliary system was found to be sterile in 58 patients (78%). fifteen patients had 35 isolates of aerobic and facultative bacteria. the most common ones were klebsiella, enterococcus, and escherichia coli. the only anaerobe isolated was clostridium perfringens. eight of 17 patients (47%) with acute cholecystitis and five of 49 patients (10%) with chronic cho ... | 1977 | 195558 |
| action of highly purified phospholipases on blood platelets. evidence for an asymmetric distribution of phospholipids in the surface membrane. | | 1977 | 195603 |
| decreased iodination of the red cell surface following phospholipase c treatment. | human red blood cells were treated with phospholipase c from clostridium welchii. lipase concentrations which produced less than 1% hemolysis and 10-15% hydrolysis of the membrane phospholipids reduced markedly (greater than 80%) the accessibility of membrane proteins to the external surface as measured by lactoperoxidase-catalyzed iodination. | 1977 | 195610 |
| icmsf methods studies. viii. comparative study for the enumeration of clostridium perfringens in foods. | four methods were compared in an international comparative study for the enumeration of clostridium perfringens: the sfp (shahidi-ferguson perfringens) agar (a), tsc (tryptose-sulfite-cycloserin) agar (b), sc (sulfite-cycloserine) agar (c), and neomycin blood agar (d) methods. the confirmed c. perfringens counts were slightly lower for d than for a-c. the percentages of presumptive colonies confirmed as c. perfringens were essentially the same in each method. the relative numbers of nonspecific ... | 1977 | 195698 |
| enterotoxin formation by clostridium perfringens type a studied by the use of fluorescent antibody. | fluorescein isothiocyanate-conjugated antibody to purified enterotoxin of clostridium perfringens was used to study the intracellular formation of enterotoxin by this organism. enterotoxin was detected at 4 h of growth at the end of the cell containing forespore. with the development of the spore, enterotoxin accumulation continued and involved the entire length of the cell until its lysis with the release of enterotoxin and mature spore. the spores did not contain demonstrable enterotoxin. only ... | 1977 | 195700 |
| detection of enterotoxin in faeces and anti-enterotoxin in serum after clostridium perfringens food-poisoning. | | 1977 | 195924 |
| spore coat protein and enterotoxin synthesis in clostridium perfringens. | polyacrylamide gel profiles of clostridium perfringens spore coat protein revealed four and occasionally five components. pulse-chase experiments indicated that synthesis of coat protein polypeptide and enterotoxin was an early sporulation event. however, maximum synthesis occurred coincident with the onset of heat resistance. | 1977 | 195933 |
| monoplace hyperbaric oxygen therapy for gas gangrene. | untreated gas gangrene is a fulminating infection that can lead to extensive tissue necrosis and death. hyperbaric oxygen, when used with antibiotics and surgical debridement, can lead to decreased mortality. nine patients with gas gangrene proven by positive clostridial cultures have been treated at st luke's hospital of kansas city with a mortality of 11.1%. a case of gas gangrene developing from a perirectal abscess is presented. | 1977 | 196119 |
| [value of bacterioscopy in diagnosis of gas gangrene]. | | 1977 | 196134 |
| antitoxin response in cattle vaccinated with clostridium perfringens type c toxoid. | | 1977 | 196381 |
| air bubbles present an embalming dilemma. | | 1977 | 196429 |
| [fecal flora of man. v. communication: the fluctuation of the fecal flora of the healthy adult (author's transl)]. | seven fecal specimens from each of healthy persons, aged 25 to 42 years, during the period of 2 months were analyzed to obtain the knowledge on the day-to-day variation in the same person and the person-to-person variation. irrespective of individuals, bacteroidaceae, catenabacteria (eubacteria and strict anaerobic lactobacilli), peptostreptococci (anaerobic gram positive cocci) and bifidobacteria constituted the predominant flora. spirillaceae, megasphaerae and clostridia (except cl. perfringen ... | 1977 | 196456 |
| cutaneous abscesses. anaerobic and aerobic bacteriology and outpatient management. | specimens from 135 cutaneous abscesses in outpatients were cultured anaerobically and aerobically. of these, 4% were sterile and 29% yielded pure cultures, predominantly of staphylococcus aureus. aerobic species were isolated from all anatomic areas. anaerobes were found with a frequency comparable to aerobes in all nonperineal areas except the hand. in contrast, abscesses in the perineal region contained a greater variety and frequency of anaerobes. only two patients were febrile. all abscesses ... | 1977 | 196534 |
| [the actions of low-molecular fragments of substrate and their analogs on the activity of isoenzymes of phospholipase c from clostridium perfringens]. | the interaction of the lecithin molecule fragments and their analogues with phospholipase c cl. perfringens was studied by gel-diffusion in agarose-lecithin gels. it was found intense inhibition of phospholipase c activity in the presence of cathionic compounds; this phenomenon shows the existence of anionic centre in the active site of enzyme. the esteric centre is probably hydrophobic nature and is not capable to bind the negatively charged groups. however, phosphoserine, phosphothreonine, gam ... | 1977 | 196687 |
| [changes in the electric activity of the cerebral cortex in poisoning caused by cl. perfringens toxin type a]. | the electrical activity of the cerebral cortex was recorded in cats under mild nembutal anesthesia (15-20 mg/kg of body weight) during the development of cl. perfringens, type a, toxin poisoning (the toxin was injected intramuscularly in a dose of 100 mld per kg of body weight). two phases of the changes in the electrical activity of the cerebral cortex were noted. the first phase was attended by the desynchronization of the electrical activity, persistence of the induced potentials and of the r ... | 1976 | 196701 |
| gas gangrene and related infection: classification, clinical features and aetiology, management and mortality. a report of 88 cases. | the clinical features of gas gangrene and related infection seen in 88 patients over a 10-year period are described. it is suggested that clostridial infection could be simply classified as either 'gas-forming' or 'non-gas-forming'. the gas-forming group represents the more severe form of infection. non-clostridial gas gangrene may present in a variety of forms. the anaerobic streptococcus was the organism most frequently responsible, but these cases were indistinguishable from clostridial infec ... | 1977 | 196711 |
| acute clostridial enteritis. | | 1977 | 196815 |
| incorporation of the 1-pro-r and the 1-pro-s hydrogen atoms of ethanol into steroids and phosphatidylcholines in vivo. | the transfer of deuterium from chiral 1-monodeuteroethanols to various metabolites formed in the liver was studied in order to investigate the coupling of metabolic reductions to the alcohol dehydrogenase and the aldehyde dehydrogenase reactions. the ethanols were administered to female bile fistula rats for 10 h. the hydrogen at c-2 in the glycerol moiety of newly formed phosphatidylcholine molecules in bile, liver and plasma was derived to 22-25% from the 1-pro-r position and to 5-6% from the ... | 1977 | 196853 |
| [evaluation and determination of the antibacterial properties of pesticide combinations]. | | 1977 | 196981 |
| immobilized clostridium perfringens neuraminidase. substrate cleavage and enzyme release during incubation. | pure clostridium perfringens neuraminidase was immobilized on sepharose 4 b, azido-sepharose 4 b and controlled pore glass (cpg)- glycophase using different coupling procedures. the immobilized enzyme showed increased stability under various conditions relative to the soluble enzyme. the low release of active enzyme from the supports under incubation conditions was quantitated using a highly sensitive radioactive assay. the activity of the immobilized enzyme was dependent on the nature of the su ... | 1977 | 196995 |
| preparative polyacrylamide gel electrophoresis purification of clostridium perfringens enterotoxin. | preparative polyacrylamide gel electrophoresis has been used to purify the enterotoxin of clostridium perfringens from sephadex g-100 extracts. purified toxin of high specific activity was eluted in 1 to 3 h, depending upon the length of the acrylamide gel used. recovery of biological activity with this technique ranged from 80 to 90%. the purity and physical characteristics of the toxin were similar to those previously reported for the protein purified by other methods. use of preparative elect ... | 1977 | 197021 |
| fulminant puerperal sepsis associated with aplastic anemia: the case for prophylactic antibiotic therapy. | fulminant puerperal sepsis due to clostridium perfringens occurred in a primiparous 19-year-old woman who developed aplastic anemia during pregnancy. although the risk of infectious complications among pregnant women with compromised host defenses has not been accurately determined, it appears to be increased, particularly in patients with granulocytopenia. it therefore seems reasonable to give antibiotic prophylaxis during the intrapartum period to immunosuppressed women who come to term. consi ... | 1977 | 197236 |
| [the significance of the presence of cl. perfringens in the feces and of alpha antitoxins in the serum of patients with rheumatoid arthritis (author's transl)]. | | 1977 | 197360 |
| activation of a procarcinogen to a mutagen by cell-free extracts of anaerobic bacteria. | the salmonella mutagenicity assay can be coupled to cell-free preparations derived from anaerobic bacteria (clostridium perfringens and bacteroides fragilis) to activate a procarcinogen to a mutagen. this activity is destroyed by heating and by digestion with pronase and it is sensitive to oxygen. these findings indicate that the salmonella mutagenicity assay can be adapted to the study of the role of anaerobes in the activation of carcinogens. | 1977 | 197405 |
| [gas gangrene. five cases of an endemic infectious disease (author's transl)]. | | 1977 | 197510 |
| bacterial hazards. | | 1977 | 197534 |
| [influence of animal fatigue on seeding of meat and internal organs with c1. perfringens]. | | 1977 | 197710 |
| [characteristics of the immunologic response of animals to clostridium perfringens anatoxin]. | experiments were conducted on guinea pigs, rabbits and mice (mongrel and inbred); immunogenic properties of cl. perfringens toxoids of different purity were studied. toxin neutralization and passive hemagglutination tests were used to determine the antitoxic immunity level. it appeared that in the immunization of guinea pigs and rabbits the degree of immunogenicity of the preparations increased with the elevation of their specific activity. under the same conditions both the mongrel and the inbr ... | 1977 | 197752 |
| [clostridium perfringens enterotoxin]. | | 1977 | 197754 |
| action of sphingomyelinase c and other lipid-specific agents as inhibitors of fc binding and locomotion in human leucocytes. | the binding of antibody-coated chicken erythrocytes (ea) to human blood lymphocytes and monocytes is inhibited by pretreatment of the leucocytes with sphingomyelinase c. inhibition of rosetting of neuraminidase-treated ea with neutrophils is also seen with this enzyme. the cholesterol-binding theta-toxin of clostridium perfringens and pronase also inhibit ea-rosette formation, but less strongly than sphingomyelinase. the lipid-specific agents also inhibit chemotactic migration of leucocytes to c ... | 1977 | 198359 |
| association of clostridium perfringens type a with human diarrhoeal cases. | | 1977 | 198362 |
| microbiology of new zealand feral venison. | | 1977 | 198612 |
| mastitis in a heifer caused by clostridium welchii, type "a". | | 1977 | 198940 |
| rapid detection and quantitation of clostridium perfringens enterostoxin by counterimmunoelectrophoresis. | conditions for detection and quantitation of clostridium perfringens enterotoxin by counterimmunoelectrophoresis are described. as little as 0.2 microgram of enterotoxin per ml could be detected. the test was found to be rapid, sensitive, specific and easy for the detection and quantitation of enterotoxin. | 1977 | 199110 |
| increased spore yields of clostridium perfringens in the presence of methylxanthines. | the methylxanthines caffeine, theophylline, and isobutylmethylxanthine greatly increased spore yields of clostridium perfringens strains fd-1, ps52, and ps49 when grown on duncan-strong medium or on a new casein-digest medium. four other strains (ka3, and national collection of type cultures strains 8798, 8238, and 10240) failed to show any significant increase when tested under similar conditions. the degree of sporulation increase was influenced by the carbohydrate energy source in some strain ... | 1977 | 199111 |
| dialysis technique for containment of microbial populations inoculated into food systems. | a technique utilizing dialysis tubing was developed for the containment of microbial populations introduced into a food system. the entrapment of the inoculum was accomplished while reducing interference with interactions between the sample and the surrounding environment. | 1977 | 199112 |
| thermal inactivation of ileal loop-reactive clostridium perfringens type a strains in phosphate buffer and beef gravy. | the thermal resistance of spore crops produced from each of two ileal loop-reactive strains of clostridium perfringens type a was determined in two suspending vehicles consisting of 0.067 m (ph 7.0) phosphate buffer and a commercial beef gravy. d115.6 values obtained in buffer and enumerated after pretreatment with sodium ethylenediaminetetraacetate and recovery in plating medium containing lysozyme were two- to threefold greater than those obtained without this treatment. d115.6 values obtained ... | 1977 | 199113 |
| antibody formation in mice in various genotypes after immunization with highly purified perfringens alpha-toxoid. | | 1976 | 199137 |
| clostridial wound infection: a hazard of small-bowel decompression. | | 1977 | 199149 |
| the substrate specificities of endo-beta-n-acetylglucosaminidases cii and h. | | 1977 | 199169 |
| structural studies on epsilon-prototoxin of clostridium perfringens type d. localization of the site of tryptic scission necessary for activation to epsilon-toxin. | | 1977 | 199192 |
| theta-toxin of clostridium perfringens. i. purification and some properties. | theta-toxin, an oxygen-labile hemolysin produced by clostridium perfringens, was purified 3300 fold from culture filtrate by successive chromatography on deae-sephadex a-50 and sephadex g-150. the purified toxin gave two distinct bands in disc electrophoresis, while the same material, after mild reduction with dithiothreitol, yielded a single band, indicating that the purified theta-toxin contained, as well as a reduced, active form, an oxidized, inactive form of toxin. these two forms of the to ... | 1977 | 199270 |
| [effect of aminazin on changes in the electrical activity of the cerebral cortex under the influence of the toxin c1. perfringens type a]. | experiments on cats revealed that injection of chlorpromazine (3 mg/kg of body weight, intramuscular) one hour prior to the cl. perfingens toxin type a injection prevented the appearance of desynchronization of the electrical activity of the cerebral cortex usually observed during the first phase of intoxication. chlorpromazine injection postpones the appearance of the electrical activity depression phase during the second phase and prolonged the animals' life span from 1.5 to 2 times. the effec ... | 1977 | 199290 |
| processive and synchronous mechanism of polynucleotide phosphorylase phosphorolysis. a comparison between experimental results and those calculated from a theoretical study. | | 1977 | 199439 |
| regional localization of activity of clostridium perfringens type a enterotoxin in the rabbit ileum, jejunum, and duodenum. | rabbit ileal, jejunal, and duodenal loops were exposed to purified enterotoxin from clostridium perfringens type a and then perfused for comparative analysis of effects of the enterotoxin on each region of the intestine. ileal loops responded with enhanced net secretion of fluid and sodium, inhibition of chloride and glucose uptake, and substantial sloughing of epithelial cells. the jejunum responded with fluid secretion, enhancement of sodium secretion only during the first 20 min, inhibition o ... | 1977 | 199674 |
| application of 13c-nmr spectroscopy to in vitro analysis of enzyme kinetics. | the conversion of d,l-alpha-13c-histidine to similarly labeled alpha-13c histamine by bacterial and mammalian histidine decarboxylase was studied by 13c-nmr spectroscopy and glc-mass spectrometry. the results obtained with the partially purified bacterial enzyme were in essentially perfect agreement with results obtained simultaneously with a standard radioisotopic method using carboxyl-labeled-14c-l-histidine. for a crude tissue preparation of the mammalian enzyme, the radioisotopic method in ... | 1977 | 199715 |
| synthesis and biological activity of two metabolites of 1-methyl-5-(1-methylethyl)-2-nitro-1 h-imidazole, an antiprotozoal agent. | 5-(-hydroxyl-1-methylethyl-)-1-methyl-2-nitro-1 h-imidazole (6) and 5-(1,2-dihydroxyl-1-methylethyl)-1-methyl-2-nitro-1 h-imidazole (9) are the principal metabolites found in urines of animals (mice, rats, and dogs) treated with 1-methyl-5-(1-methylethyl)-2-nitro-1 h-imidazole (1), an effective antitrichomonas agent. these two metabolites have been synthesized. compound 6 was found to be less toxic than the parent compound 1 and to possess essentially the same activity against trichomonas vagina ... | 1977 | 199729 |
| [characteristics of alpha-toxin of clostridium perfringens and its role in the pathogenesis of gas gangrene (author's transl)]. | | 1977 | 199761 |
| [study of the variability of cl. perfringens type d]. | | 1977 | 199982 |
| [biologic-economic study of processes for cultivating clostridium perfringens. ii. comparison of the effectiveness of actual and predicted processes for cultivating microorganisms]. | the authors analyzed the efficacy of actual and prognosticated processes of cl. perfringens, type a, cultivation: periodic, semicontinuous, continuous, and combined monocyclic. comparative study permitted to choose the economically optimal processes, without any expenditure for their realization. monocyclic combined process which could provide a great amount of active toxin at short periods proved to be the most advantageous among the cultivation processes studied. | 1977 | 200039 |
| effect of microwave radiation on cells treated with membrane-injuring substances. | wish cells grown in vitro were pretreated with subcytotoxic concentrations of digitonin, cortisol and purified bacterial toxins -- staphylococcal beta-haemolysin or clostridium perfringens alpha-toxin and irradiated with 3 ghz electromagnetic wave (microwaves) at the field power densities 5 or 40 mw/cm2. at 40 mw/cm2 increase in temperature of the culture medium of about 2-3 degrees c was noted, while at 5 mw/cm2 no detectable increase in temperature was found. control and pretreated wish cells ... | 1977 | 200452 |
| physical changes in the epsilon prototoxin molecule of clostridium perfringens during enzymatic activation. | enzymatic activation of clostridium perfringens epsilon prototoxin removed a small basic part of the molecule, causing a slight change in molecular weight (32,700 to 31,200) and a large change in isoelectric point (from ph 8.02 into fractions of 5.36 and 5.74). | 1977 | 200566 |
| anaerobes in human biliary tracts. | during a 2-year period, 1,892 patients underwent biliary tract surgery at the mayo clinic. both aerobic and anaerobic cultures of bile were performed in 371 of these patients. sixty-nine percent of the cultures were positive, and 41% (117) of these grew anaerobes, although they were present in pure culture only twice. mixed cultures most commonly contained four different organisms (three aerobes and one anaerobe). bacteroides fragilis was the single most commonly isolated anaerobe and ranked fou ... | 1977 | 200634 |
| antimicrobial agent-induced diarrhea--a bacterial disease. | | 1977 | 200682 |
| clostridial myonecrosis of the postpartum uterus with radiologic diagnosis. | a case of clostridial uterine infection following prolonged labor and vaginal delivery is presented. uterine myonecrosis with gas formation was diagnosed by radiologic studies. antimicrobial therapy with megadosage penicillin was initiated and total abdominal hysterectomy performed. three episodes of cardiac arrest occurred during surgery. resuscitation was carried out with recovery. | 1978 | 200877 |
| [loss of myolysis and decrease of toxicity in clostridium perfringens type a mutants: in vitro study of a relationship among hemolysins, growth, and metabolism]. | | 1977 | 200986 |
| the in vitro and in vivo activity of taurolin against anaerobic pathogenic organisms. | an antibacterial agent, taurolin, formed by the combination of 2 molecules of taurine with 3 molecules of formaldehyde has been tested against anaerobes in vitro and in vivo. in vitro, taurolin is highly active against bacteroides species and anaerobic streptococci as measured by the minimum inhibitory concentration. in vivo, small dosages of taurolin given intraperitoneally increase the mortality in mice with bacteroides peritonitis. however, by increasing the dosage of taurolin, survival is pr ... | 1977 | 201040 |
| [isolation of clostridium perfringens from the muscles and internal organs of animals]. | | 1977 | 201077 |
| [activity of anatoxins against infectious enterotoxemia of sheep, prepared with different adjuvants]. | | 1977 | 201079 |
| [prevention of anaerobic gastrotoxemia of lambs]. | | 1977 | 201080 |
| vaccination of cattle and sheep with a combined clostridium perfringens types c and d toxoid. | cattle and sheep (30 each) were vaccinated with a combined clostridium perfringens type c and c perfringens type d toxoid. vaccination and blood sample collections were made every 2 weeks over a period of 8 weeks. increases in antitoxin titers occurred after the 2nd administration of the 2.0-ml combined product. highest titers were recorded when the 2nd vaccinal dose was given 2 weeks after the first. this confirms reports that response to clostridial antigens is greater when the 2nd immunizing ... | 1977 | 201186 |
| [microcirculatory changes following the combined action of the toxin of c1. perfringens type a and biological products of c1. butyricum]. | | 1977 | 201314 |
| purification of beta-toxin from clostridium perfringens type c. | beta-toxin was purified about 340-fold from culture supernatant fluid of clostridium perfringens type c with a yield of about 24% in terms of biologically active beta-toxin. the purification involved ammonium sulfate fractionation, gel filtration through sephadex g-100, isoelectrofocusing in a ph 3 to 6 gradient, and immunoaffinity chromatography. the purified beta-toxin gave a single band on polyacrylamide gel electrophoresis. | 1977 | 201565 |
| experimental diarrhoea in human volunteers following oral administration of clostridium perfringens enterotoxin. | | 1977 | 201601 |
| hydrolytic action of phospholipases on bacterial membranes. | substrate specificities of phospholipases c[ec 3.1.4.3] from clostridium novyi, clostridium perfringens, bacillus cereus, and pseudomonas aureofaciens were studied under the same conditions. phospholipases c from clostridium novyi and bacillus cereus show wide substrate specificities while those of clostridium perfringens and pseudomonas aureofaciens show relatively narrow specificities. on the basis of these results, the hydrolytic actions of these phospholipases on membrane lipids of escherich ... | 1977 | 201610 |
| increased numbers of heat-resistnat spores produced by two strains of clostridium perfringens bearing temperate phage s9. | sporulation kinetics and spore heat resistance data were compared for a lysogenic strain of clostridium perfringens, s9, before and after curing with ultraviolet irradiation. the cured strain showed the same growth rate in broth media as the lysogenic strain but took 6 h longer to form refractile spores. for lysogenized and cured strains the percentages of refractile spores produced that were heat-resistant (80 degrees c for 15 min) were 50 and 0.2, respectively. when reinfected with the tempera ... | 1977 | 201726 |
| [biological-economic study of the clostridium perfringens cultivation processes. 1. value of the phasic portrait in the prognosis of the processes of microbial cultivation]. | | 1977 | 202118 |
| [cultural properties of c1. perfringens resistant and sensitive to morphocycline]. | | 1977 | 202190 |
| [immunogenicity and thymus-dependence of the polymerized alpha-toxoid of clostridium perfringens]. | polymeric alpha-toxoid with a molecular weight of 450 000--600 000 was obtained by condensation of alpha-toxoid of cl. perfringens, type a, with glutaric aldehyde. experiments on guinea pigs showed that in the adsorbed preparations the immunogenic properties of both monomeric and polymeric alpha-toxoids are practically identical. the primary immune response after the immunization with nonadsorbed antigens was 3 times greater than that induced by the monomer. polymerization of alpha-toxoid failed ... | 1977 | 202354 |
| [pathogenesis of necrotizing enteritis caused by clostridium perfringens]. | | 1977 | 202542 |
| studies on nitrate reductase of clostridium perfringens. purification, some properties, and effect of tungstate on its formation. | nitrate reductase (nar) linked to reduced methyl viologen from clostridium perfringens was purified by ammonium sulfate precipitation. deae-cellulose chromatography, disc electrophoresis on polyacrylamide gel, and triple deae-sephadex chromatography. the specific activity was increased 1,200-fold with a yield of 9%. the purified preparation was nearly homogeneous in disc electrophoresis. it was brown, and its spectrum showed a slight shoulder near 420 nm as well as a peak at 280 nm. the molecula ... | 1977 | 202590 |
| microbiological investigation of meat wholesale premises and beef carcases in johannesburg. | microbiological surveillance by swabbing meat wholesaler premises revealed ineffective cleaning and build-up of bacteria. proper cleaning, sanitation and handling resulted in a vast improvement during 1975-77. beef samples from the neck of carcases in the wholesale trade were investigated by microbiological methods. excessive total bacterial counts were obtained from numerous carcases. most carcases carried coliform organisms. roughly 90% of carcases were contaminated with e. coli i; counts exce ... | 1977 | 202708 |
| the effect of antibiotics, primary and secondary closure on clostridial contaminated open fracture wounds in rats. | in a study of experimentally induced open tibial or femoral fractures in rats, after either closing or leaving the wounds open, the animals were given: no antibiotic, cephalothin (keflin), or penicillin. the rats with wounds closed primarily and receiving no antibiotics had the highest mortality rate (11 of 25) from experimentally produced clostridial myonecrosis. the lowest overall mortality rate (5 of 99) was found in the penicillin-treated groups. the higher mortality rate in the femur fractu ... | 1978 | 202725 |
| [toxicity and sporulation of cl. perfringens strains type a]. | | 1977 | 202818 |
| [effect of various antibiotics, disinfectants and uv light on the viability of vegetative cells and spores of cl. perfringens type a]. | | 1977 | 202819 |
| [new findings on various bacteria and their toxins as possible causative agents of food poisoning]. | | 1977 | 203102 |
| dissimilatory nitrate reduction in clostridium tertium. | fermentation balance studies were carried out on clostridium tertium grown with and without nitrate in the medium. nitrate reduction increased the efficiency of energy produced from glucose by permitting the utilization of additional sites of substrate level phosphorylation. the effect was even more dramatic in c. tertium than in c. perfringens, with increased cell yields of about 30% being observed in the former compared with 20% in the latter. unlike c. perfringens, c. tertium responded to the ... | 1977 | 203129 |
| media supplemented with nalidixic acid for the isolation of gram negative anaerobic bacteria. | the minimum inhibitory concentrations (mic) of nalidixic acid were determined for thirty-four strains of clostridium sp. by an agar dilution technique. the mic range of clostridium perfringens ran from 0.03 microgram/ml to 64 microgram/ml, according to a bimodal distribution. one half of the strains was inhibited by 0.25 microgram/ml. 64 microgram/ml was able to inhibit all the strains tested. the author suggests a systematic utilization of media with and without 40 microgram/ml nalidixic acid. ... | 1977 | 203144 |
| inhibition of noradrenaline-stimulated lipolysis and cyclic amp accumulation in isolated rat adipocytes by purified phospholipase c and theta-toxin from clostridium perfringens. | purified phospholipae c (phosphatidylcholine cholinephosphohydrolase, ec 3.1.4.3) and theta-toxin from clostridium perfringens both inhibited noradrenaline-stimulated lipolysis and cyclic amp accumulation in isolated rat adipocytes in a dose-dependent manner. the action of phospholipase c was gradual in onset, while the effect of theta-toxin was almost immediate. phospholipase c, but not theta-toxin, hydrolyzed membrane phospholipids and inhibited adenylate cyclase (ec 4.6.1.1) in a crude membra ... | 1978 | 203164 |
| intraleukocytic spore formation and leukocytic vacuolization during clostridium perfringens septicemia. | the case of a patient with fulminant clostridium perfringens septicemia and intravascular hemolysis is presented. the organisms in the blood stream were in sufficient quantity to be detected on a peripheral blood smear, and some were found to be within leukocytes. in vivo spore formation by c. perfringens is thought to be uncommon; however, in this patient probable spore formation by this organism was observed within peripheral blood leukocytes. the peripheral polymorphonuclear leukocytes from t ... | 1977 | 203181 |
| phospholipid dependence of udp-glucuronyltransferase. | very extensive hydrolysis of phospholipids with pure bacillus cereus phospholipase c at 5 degrees c greatly inhibited the maximum demonstrable rate of glucuronidation of p-nitrophenol by udpglucuronyltransferase in guinea pig liver microsomes. lysophosphatidylcholine restored much of the inhibited activity but non-phospholipid surfactants or hydrolysis of diglycerides failed to reactivate. phospholipid depletion likewise inhibited o-aminophenol glucuronidation and phospholipids restored activity ... | 1978 | 203315 |
| detection of alcohols and volatile fatty acids by head-space gas chromatography in identification of anaerobic bacteria. | a head-space gas chromatographic technique for the analysis of volatile bacterial metabolites is described. bacteroides fragilis, clostridium perfringens, and propionibacterium acnes, cultured in a glucose-containing peptone yeast extract medium, were studied. the head-space technique was compared with the injection of the complete liquid culture medium, and solvent extracts thereof, into the gas chromatograph. volatile fatty acids could be detected by all three methods, whereas alcohols produce ... | 1978 | 203602 |
| microbiology of new zealand farmed venison. | | 1977 | 203773 |
| [two outbreaks of "clostridium perfringens" food poisoning: epidemiological remarks (author's transl)]. | two outbreaks of cl. perfringens food poisoning which occurred in florence during 1976 have been described. the first one involved three hundred primary school children; processed re-heated turkey meat was thought to have been the vehicle of infection in the school meal. the clinical symptoms consisted of mild diarrhoea in all cases and the duration of the illness was about 12 hours. the possible part played by food storage temperature, post-cooking periods and food trolleys in the spread of inf ... | 1977 | 204262 |
| anaerobic septicemia after transrectal prostatic biopsy. | transrectal biopsy of the prostate resulted in anaerobic septicemia in two patients, despite parenteral gentamicin sulfate prophylaxis. bacteroides fragilis sepsis developed subacutely in one patient having a postbiopsy pelvic abscess. clostridium perfringens sepsis occurred fulminantly in another patient 24 hours after biopsy of a gland extensively involved with adenocarcinoma. these cases indicate a potential hazard of sepsis due to anaerobic contamination with rectal microflora at the time of ... | 1978 | 204264 |
| clostridium welchii septicaemia after intrauterine caesium insertion. | | 1978 | 204393 |