| cc-1065 (nsc-298223), a new antitumor antibiotic. production, in vitro biological activity, microbiological assays and taxonomy of the producing microorganism. | a new antitumor antibiotic is produced in fermentation liquors of streptomyces zelensis sp.n. the antibiotic is biologically active at extremely low concentrations. at 40 pg/ml, it inhibited 90% of the growth of l1210 cells in culture in tube dilution assays. the minimal inhibitory concentrations against gram-positive bacteria is between 1 approximately 10 ng/ml, while these values for gram-negative bacteria and fungi are mostly under 1 microgram/ml. a microbiological assay with bacillus subtili ... | 1978 | 104946 |
| specific inhibition of outgrowth of bacillus subtilis spores by novobiocin. | spores of a bacillus subtilis mutant temperature sensitive in deoxyribonucleic acid (dna) replication proceeded through outgrowth at the nonpermissive temperature to the same extent as the wild-type parent spores. in contrast, the dna synthesis inhibitor novobiocin completely prevented spore outgrowth while displaying a marginal effect on logarithmic growth during one generation time. inhibition of outgrowth by novobiocin occurred in the absence of dna replication, as demonstrated in an experime ... | 1979 | 108254 |
| export of extracellular levansucrase by bacillus subtilis: inhibition by cerulenin and quinacrine. | bacillus subtilis b secretes an inducible, extracellular enzyme, levansucrase. inhibition studies were undertaken to investigate the possible mechanism of release of this enzyme. the antibiotic cerulenin, at a concentration of 10 micrograms/ml, totally inhibited de novo lipid synthesis in b. subtilis b for at least 1 h, while only slightly reducing protein and rna synthesis. at this concentration cerulenin, added concomitantly with the inducer sucrose, prevented the release of levansucrase for a ... | 1979 | 108256 |
| characterization of a succinate dehydrogenase complex solubilized from the cytoplasmic membrane of bacillus subtilis with the nonionic detergent triton x-100. | a succinic dehydrogenase (sdh) complex has been purified from triton x-100-solubilized membranes from bacillus subtilis by precipitation with specific antibody. radioactively labeled precipitated complex was analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis followed by autoradiography of the gels. the complex contained equimolar amounts of three polypeptides with approximate molecular weights of 65,000, 28,000, and 19,000. five succinic dehydrogenase-negative mutants, belongi ... | 1979 | 108258 |
| role of heme in synthesis and membrane binding of succinic dehydrogenase in bacillus subtilis. | a 5-aminolevulinic acid-requiring mutant of bacillus subtilis was isolated. when the mutant is shifted from medium containing 5-aminolevulinic acid to medium lacking this growth factor, the bacteria continued to grow at undiminished rate for about three generations. the membranes from these bacteria contained severely reduced amounts of cytochrome. the mutant was used to study the role of heme synthesis on synthesis and membrane binding of succinic dehydrogenase (sdh). the amount of sdh in whole ... | 1979 | 108259 |
| morphology and patterns of protein synthesis during sporulation of bacillus subtilis eryr spo(ts) mutants. | erythromycin-resistant (eryr) mutants of bacillus subtilis 168 fail to sporulate at high temperature (47 degrees c) but sporulate normally at 30 to 35 degrees c. they also fail to sporulate at any temperature in the presence of 2.5 micrograms of erythromycin per ml. neither of these nonpermissive conditions appears to affect vegetative growth, and the periods of sensitivity to both conditions extend from 40 to 90% of the sporulation period. at 47 degrees c, net incorporation of methionine and ph ... | 1979 | 108263 |
| studies of the high molecular weight penicillin-binding proteins of bacillus subtilis. | seven or eight penicillin-binding proteins (pbps) were detected in bacillus subtilis membranes. by introducing covalent affinity chromatography employing cephalosporins as ligands, milligram amounts of three high molecular weight pbps (pbp 1 ab, mr = 120,000; pbp 2b, mr = 94,000; and pbp 4, mr = 78,000) were obtained without any contamination of the major pbp 5, the d-alanine carboxypeptidase. small amounts of pure pbp 2b could be isolated by manipulation of the affinity chromatography condition ... | 1979 | 108285 |
| mutants of bacillus subtilis affected in spore outgrowth. | six mutants of bacillus subtilis 168 that are temperature-sensitive in spore outgrowth were isolated. the outgrowth process proceeds normally at 35 degrees c, but at the non-permissive temperature (47 degrees c) it is arrested at a specific stage characteristic for each mutant strain. the mutants are not altered in vegetative growth whether at 35 degrees c or at 47 degrees c. they were characterized for their ability to synthesize rna, proteins and dna during outgrowth. a mutant defective in spo ... | 1979 | 108356 |
| a bacillus subtilis mutant requiring dipicolinic acid for the development of heat-resistant spores. | a bacillus subtilis mutant is described which forms heat-resistant spores only in the presence of external dipicolinic acid (dpa). the mutation, dpa-1, is localized in a new sporulation locus, linked to pyra. the dpa-1 strain is unable to synthesize dpa but can incorporate external dpa. the amount of dpa incorporated, the frequency of heat-resistant spores and their degree of resistance are all dependent on the concentration of external dpa. spores of dpa- 1 strains exhibit normal resistance to ... | 1979 | 108357 |
| decadent sporulation mutants of bacillus subtilis. | in decadent sporulation mutants, sporulating populations are heterogeneous: the cells reach successive chemical and physical resistances with progressively decreasing frequencies. each decadent mutant can be characterized by the shape and slope of the curve describing the frequency of cells resistant to various agents ('the resistance spectrum'). in some mutants the resistance spectrum decreases progressively from xylene resistance to heat resistance; in other mutants it decreases rapidly betwee ... | 1979 | 108358 |
| fractionation of ribosomal particles from bacillus subtilis. | | 1979 | 108506 |
| reconstitution of active 50 s ribosomal subunits from bacillus lichenformis and bacillus subtilis. | | 1979 | 108508 |
| microbiological biosynthesis of biotin. | | 1979 | 108512 |
| induction of sporulation in developmental mutants of bacillus subtilis. | | 1979 | 108515 |
| discontinuous duplication of both strands of virus 2c dna. | | 1979 | 108519 |
| [lipolytic activity of the spore-forming bacterium, bacillus subtilis]. | | 1979 | 108527 |
| [effect of exogenous nucleodepolymerases on bacillus subtilis multiplication]. | the effect of exogenous nucleodepolymerases on cell growth was studied with bacillus subtilis. nucleodepolymerases of microbial and animal origin stimulated growth of the culture. the stimulating action of dnaases depended on the dose of the enzyme added to the growth medium and on the phase of the cultural growth. acceleration of the cultural growth correlated with intensification of dna synthesis in the bacterial cells. | 1979 | 108529 |
| gentic engineering for practical application. | genetic engineering has ushered in a new era in biology. although many problems are still to be solved, there are examples that point to a possible later application for the benefit of mankind: bacteria can be manipulated to degrade crude-oil spillages, to produce human insulin and to bind nitrogen from the air. if all the bacteria that are indigenous to agricultural soils could be made to bind nitrogen, an increase in soil fertility might well result. | 1979 | 108601 |
| antibody to b. subtilis dna polymerase iii: use in enzyme purification and examination of homology among replication-specific dna polymerases. | bacillus subtilis dna polymerase iii (pol iii), an arylhydrazinopyrimidine-sensitive, replication-specific enzyme, was used to generate a non-precipitating rabbit antibody which specifically inhibited pol iii activity in vitro. the antibody was used to examine structural relationships among several dna polymerases, and it was linked covalently to agarose; the antibody:agarose was employed to develop a rapid, selective method of purification of catalytically active b. subtilis pol iii. | 1979 | 108667 |
| [on the antimicrobial activity of propolis and propolis constituents (author's transl)]. | after a survey of the literature on the antimicrobial activity of the bee product propolis, the authors discuss their own findings as compared to the chemotherapeutical agents streptomycin, oxytetracycline, chloramphenicol, nystatin, griseofulvin and sulphamerazine. according to the results obtained by testing 25 isolated constituents on bacillus subtilis, staphylococcus aureus, candida albicans and trichophyton mentagrophytes, the antimicrobial properties of this mixture of natural substances a ... | 1979 | 108687 |
| investigation into the effects of cell wall antigens of gram-positive bacteria on lymphocyte stimulation and on cell-mediated cytotoxicity. | the effect of staph, epidermidis and bac. subtilis cell walls as well as of cell wall teichoic acid, n-acetyl-muramic acid, n-acetyl-d-glucosamine, d-alanine, and dl-alpha, epsilon-diaminopimelic acid on lymphocyte stimulation and on cell-mediated cytotoxicity has been studied. bac. subtilis cell wall preparations, n-acetyl-d-glucosamine and teichoic acid showed a slight but significant effect on the mitogenic response of pig lymphocyte cultures. when studied in combination with the mitogens pha ... | 1979 | 108870 |
| [biosynthesis of extracellular proteolytic enzymes by bac. mesentericus strain 90]. | | 1978 | 108924 |
| [bacterial strains producing enzymes with milk-clotting activity. xiii. alakine protease from the enzyme complex of bac. mesentericus strain m]. | | 1978 | 108925 |
| an improved microbiological procedure for detection of streptomycin residues in milk and animal tissues. | | 1979 | 108927 |
| [participation of the antibiotics of bac. pumilus and bac. subtilis in the regulation of bacterial spore formation]. | sporulation and antibiotic production, as well as the effect of exogenic antibacterial substances on bacterial sporogenesis were studied in various strains of bac. pumilus and bac. pumilus and bac. subtilis. the bacteria were grown on a solid sporulation medium with and without the antibiotics. after 5-day incubation the presence of refractyl spores was determined with a phase-contrast method. it was found that in the strains of bac. pumilus producing antibacterial substances the sporulation was ... | 1979 | 109036 |
| separation of algal mixtures and bacterial mixtures with flow-microfluorometer using chlorophyll and ethidium bromide fluorescence. | the applicability of flow-microfluorometer to separate microbial cells was demonstrated with algal and bacterial cells. algal mixtures were sorted according to the natural chlorophyll fluorescence and the bacterial mixtures were sorted according to the fluorescence of ethidium bromide-stained nucleic acid. | 1979 | 109060 |
| effects of 6-thiopurines on the transforming activity of bacillus subtilis dexoyribonucleic acid. | | 1979 | 109093 |
| interactions between bacterial membranes and peptidolipids: lysis of micrococcus luteus protoplasts by derivatives of peptidolipidic antibiotics from bacillus subtilis. | the lysis of protoplasts of micrococcus luteus has been tested with various derivatives of three peptidolipidic antibiotics: iturin a, mycosubtilin and bacillomycin l. the lytic activity is dependent to the nature of the substituting group and to the position of the substituted aminoacid residue. the acetylation of oh groups leads to a decrease of the lytic activity of the natural antibiotics. the methylation of aspartyl residues of bacillomycin l gives a strong lytic activity while natural baci ... | 1979 | 109121 |
| determination of enzyme activities with the enzyme thermistor unit. | | 1979 | 109314 |
| a method for construction of specialized transducing phage rho 11 of bacillus subtilis. | dna from a temperate phage rho 11 and chromosomal dna of bacillus subtilis 168 were digested with endonuclease ecori and then ligated with t4 polynucleotide ligase. the ligated dna fragments were used to transform a lysogenic strain, b. subtilis spoa12 lys21 hisa1 leua8 p11, and lys+, his+ or leu+ transformants were selected. the cells of each type were then mixed, grown and treated with mitomycin c; the induced phages were tested for abilities abilities to form plaques and to tranduce the auxot ... | 1979 | 109355 |
| regulation of bacillus subtilis glutamine phosphoribosylpyrophosphate amidotransferase activity by end products. | | 1979 | 109433 |
| the genome of b. subtilis phage ssp1: the topology of dna molecules. | dna molecules of b. subtilis phage spp1 exhibit terminal redundancy and are partially circularly permuted. this was established by the hybridization of selected ecori restriction fragments to single strands of spp1 dna and by an analysis of the distribution of denaturation loops in partially denatured spp1 dna molecules. deletions in ssp1 dna are not compensated by an increase in terminally repetitious dna. this finding, which is unique to spp1, is discussed in terms of a modification of the str ... | 1979 | 109737 |
| alignment of partial denaturation map of bacteriophage spp1 dna. | | 1979 | 109738 |
| the genome of bacillus subtilis phage spp1: the arrangement of restriction endonuclease generated fragments. | spp1 dna was cleaved by the restriction endonucleases, bgli, bglii, ecori, kpni, smai, and sali. the molecular weights of the dna fragments obtained by single enzyme digestion or by consecutive digestion with two enzymes were determined by electron microscopic measurements of contour length and by gel electrophoresis. the major fragments from the six digests could be ordered to give a consistent restriction map of spp1. the electropherograms of several digests indicated that certain fragments oc ... | 1979 | 109739 |
| studies on the synthesis of plasmid-coded proteins and their control in bacillus subtilis minicells. | | 1979 | 109873 |
| on the trapping of phage genomes in spores of bacillus subtilis 168. reciprocal exclusion of phages phi29 and phie during outgrowth of spores. | | 1979 | 109989 |
| subunit composition of bacillus subtilis rna polymerase during transcription. | | 1979 | 110325 |
| characterization of an inhibitor of the intracellular protease from bacillus subtilis. | a specific inhibitor of intracellular serylprotease from bacillus subtilis has been isolated from both growing and sporulating cells. like other protease inhibitors isolated from eukaryotic cells, the inhibitor from b. subtilis is a thermostable protein. a purification method is described. the molecular weight estimated by biogel filtration and sds gel electrophoresis is about 15,500. both proteolytic and esterolytic activities of intracellular protease are equally sensitive to inhibition. with ... | 1979 | 110359 |
| [subtilisin 72: a serine protease from bac. subtilis strain 72 - an enzyme similar to subtilisin carlsberg]. | subtilisin 72, a serine proteinase secreted by bac. subtilis strain 72 was purified by covalent chromatography on sepharose sorbent containing p-(omega-aminomethyl)phenylboronic acid as a ligand. the homogeneity of subtilisin 72 was confirmed by isoelectrofocusing in a thin layer of polyacrylamide gel (pl 8.6). the amino acid composition of this enzyme is different from that of other subtilisins, e. g. subtilisin carlsberg. the n = terminal amino acid sequence of subtilisin 72 traced up to the 3 ... | 1979 | 110360 |
| chemical and ultrastructural examination of lead-induced morphological convertants of bacillus subtilis. | actively-growing bacillus subtilis 168 cells, exposed to lead nitrate, had only slightly decreased ability to bind the divalent cation magnesium. the nature and quantity of the major cell wall metal binding components, teichoic and teichuronic acids, and the carbohydrate constituents of the peptidoglycan remained relatively constant. purified cell walls, isolated from cells exposed to lead for 6 and 13 days, retained 9.9 micrograms pb+2 and 3.5 micrograns pb+2/mg cell wall, respectively. the occ ... | 1979 | 110478 |
| selective association of the chromosome with membrane in a stable l-form of bacillus subtilis. | a stalbe l-form (sal-1) of bacillus subtilis was found to have retained a markedly modified chromosome-membrane association when compared to intact cells. the membrane-deoxyribonucleic acid complex of the l-form was similar to that of its parental strain in quantity and stability. genetic analysis of the l-form membrane-deoxyribonucleic acid complex revealed enrichment for markers close to the replication origin, but not for internal markers, indicating preferential attachment of the origin of c ... | 1979 | 110772 |
| deoxyribonucleic acid sequence common to staphylococcal and streptococcal plasmids which specify erythromycin resistance. | plasmids from erythromycin-resistant staphylococcus aureus, streptococcus sanguis, and streptococcus faecalis show deoxyribonucleic acid sequence homology. the homologous sequences can be localized to specific restriction endonuclease fragments, which in the case of s. aureus plasmid pi258 involves a single fragment from either ecori or hindiii digest known to contain the erythromycin resistance determinant. complementary ribonucleic acid probes prepared from s. aureus plasmid pi258 and s. sangu ... | 1979 | 110774 |
| gap-filling repair synthesis induced by ultraviolet light in a bacillus subtilis uvr- mutant. | deoxyribonucleic acid repair synthesis was studied in one wild-type and two mutant strains of bacillus subtilis that are defective in excision of pyrimidine dimers. the cells were irradiated with ultraviolet light, and 6-(p-hydroxyphenyl-azo)-uracil was used to block replicative synthesis, allowing only repair synthesis. one of the mutations (uvs-42) resulted in a severe inhibition of incision, dimer excision, and repair synthesis. in contrast, the other mutant (uvr-1) slowly incised and excised ... | 1979 | 110780 |
| pyrimidine dimer excision in a bacillus subtilis uvr- mutant. | a technique which allows the measurement of small numbers of pyrimidine dimers in the deoxyribonucleic acid (dna) of cells of bacillus subtilis irradiated with ultraviolet light has been used to show that a strain mutant at the uvr-1 locus is able to excise pyrimidine dimers. excision repair in this strain was slow, but incision may not be rate limiting because single-strand breaks in dna accumulate under some conditions. excision repair probably accounted for a liquid-holding recovery previousl ... | 1979 | 110781 |
| genetic recombination during transformation in bacillus subtilis: appearance of a deoxyribonucleic acid methylase. | in bacillus subtilis the ability to take up deoxyribonucleic acid (dna) and undergo genetic transformation may coincide with the induction of defective phage(s) and the expression of possibly related cryptic genes. a restriction-modification enzyme system appears to be expressed. targets of the restriction activity on the dna can be blocked my methylation catalyzed by the methyl transferase. it is shown that cellular dna becomes progressively methylated and reaches the maxium level during the pe ... | 1979 | 110783 |
| altered membrane proteins in a minicell-producing mutant of bacillus subtilis. | the bacillus subtilis minicell-producing mutant diviv-b1 has a membrane protein profile that is strikingly different from that of the other minicell-producing mutant, diviv-a1, or that of wild-type strain cu403. | 1979 | 110785 |
| introduction of host-controlled modification and restriction systems of bacillus subtilis iam1247 into bacillus subtilis 168. | bacillus subtilis iam1247 had two modification and restriction systems (bsu1247i and bsu1247ii), the former producing an isoschizomer of psti endonuclease. a transformant clone was isolated which had bsu 168, bsur, and bsu1247i systems coexisting within a genome. | 1979 | 110786 |
| genetic evidence for possible interaction between a ribonucleic acid polymerase subunit and the spo0c gene product of bacillus subtilis. | spontaneous rifampin-resistant mutants (9v rifr) were isolated from a mutant strain of bacillus subtilis, 9v, which has a spo0c mutation. whereas 90% of the 9v rifr double mutants maintained the spo0c phenotype (spo- abs +/-), the remaining 10% had the spo0a phenotype (spo- abs-). the latter mutants, termed 9v rifr spo- abs-, were revealed to have other spo0a characters, such as reduced transformability, higher sensitivity to phage phi 2, and reduced frequency of lysogenization by phage phi 105. ... | 1979 | 110788 |
| isolation and characterization of two tryptophan biosynthetic enzymes, indoleglycerol phosphate synthase and phosphoribosyl anthranilate isomerase, from bacillus subtilis. | two of the enzymes responsible for tryptophan biosynthesis in bacillus subtilis have been extensively purified. these proteins are indole-3-glycerol phosphate synthase and n-(5'-phosphoribosyl) anthranilate isomerase. by comparison to the non-differentiating enteric bacteria in which these two enzymes are fused into a single polypeptide, the isolation of the indoleglycerol phosphate synthase and phosphoribosyl anthranilate isomerase from b. subtilis has demonstrated that the two proteins are sep ... | 1979 | 110789 |
| ribonucleic acid polymerase mutation affecting glutamate synthase activity in and sporulation of bacillus subtilis. | spore formation of 15 rifampin-resistant (rifr) mutants of bacillus subtilis strain 168 was examined. as a pleiotropic effect of a rifr mutation, glutamate synthase activity was lost in these mutants. twelve of the 15 mutants examined formed as many spores as the parent, but the remaining 3 formed significantly fewer (1%) spores. one of the latter mutants characterized further (rf301) was blocked in its sporulation process at stage 0. thus, it was concluded that a certain modification of ribonuc ... | 1979 | 110792 |
| genetic location of the bacillus subtilis sup-3 suppressor mutation. | the sup-3 suppressor mutation of bacillus subtilis has been located between the aroi and mtlb loci by pbs-1 phage transduction. | 1979 | 110793 |
| increased synthesis of ribonucleotide reductase after deoxyribonucleic acid inhibition in various species of bacteria. | the specific activity of ribonucleotide reductase was found to increase significantly after deoxyribonucleic acid inhibition in seven species of bacteria investigated. this group of bacteria includes species with b12-dependent ribonucleotide reductase as well as some with an escherichia coli-type ribonucleotide reductase. | 1979 | 110794 |
| two cytoplasmic 5'-nucleotidases of bacillus subtilis k. | | 1979 | 110796 |
| transport and binding of riboflavin by bacillus subtilis. | riboflavine uptake and membrane-associated riboflavin-binding activity has been investigated in bacillus subtilis. riboflavin uptake proceeds via a system whose general properties are indicative of a carrier-mediated process: it is inhibited by substrate analogues, exhibits saturation kinetics, and is temperature-dependent. the organism concentrates riboflavin primarily as the phosphorylated cofactors fmn and fad. energy is required for uptake but whether the energy demand is required for both u ... | 1979 | 110806 |
| genetics analysis of spore germination mutants of bacillus subtilis 168: the correlation of phenotype with map location. | the isolation and characterization of 29 new germination (ger) mutants of bacillus subtilis 168 is described. these were classified, along with previously described mutants, into seven groups according to map location. the mutations in 26 gera mutants mapped between cysb and thr; detailed mapping of two of these has located them very close to citg. these mutants were deficient in germination in alanine, but responded to the germinative combination of asparagine, glucose, fructose and kcl. one ge ... | 1979 | 110906 |
| possibility for error in fda diffusion assays. | computational procedures specified for the fda single-dose diffusion assay for antibiotics may cause substantial error in estimated sample potency. an unrecognized mistake in reference solution concentration is the source of error. it is caused by correcting responses from standard and sample plates differently. the error can be avoided by correcting both standard and sample responses to the observed reference response. | 1979 | 110918 |
| lethal effect of protamine and histone on competent bacillus subtilis cells. inhibition of genetic transformation by protamine in sublethal concentration. | under experimental conditions of genetic transformation, protamine and total histone were bactericidal for bacillus subtilis cells. the abilities to cause lethality were very similar for both, either protamine or histone, with no antagonistic effects amongst these natural polycations. with both basic proteins acting simultaneously the enhancement was higher than a summation of the separate lethal effects. sublethal concentration of protamine added at the beginning of transformation time, produce ... | 1979 | 111002 |
| [isolation and properties of b. subtilis dna-binding proteins inhibiting atp-dependent deoxyribonuclease]. | the fraction inhibiting atp-dependent dnaase and some other enzyme activities was found in b. subtilis cell extracts. two methods of its isolation were elaborated. it is established that the inhibiting activity fraction represents a set of some positively charged thermostable proteins of low molecular weight (m 9000--25 000). the inhibiting effect of the proteins in question may be attributed to their ability to form a complex with dna. the complex is formed in low ionic strength conditions. the ... | 1979 | 111035 |
| mechanism of penicillin action: penicillin and substrate bind covalently to the same active site serine in two bacterial d-alanine carboxypeptidases. | it has been hypothesized that penicillin acts as a structural analog of the acyl-d-alanyl-d-alanine terminus of nascent bacterial cell wall and that it consequently binds to and acylates the active site of the enzyme(s) that crosslinks the cell wall to form an inactive penicilloyl enzyme [tipper, d.j. & strominger, j.l. (1965) proc. natl. acad. sci. usa 64, 1133-1138]. this study directly proves that penicillin acylates the active site of two penicillin-sensitive enzymes, d-alanine carboxypeptid ... | 1979 | 111240 |
| lethal effects of 32p decay on transfecting activity of bacillus subtilis phage phi e dna. | | 1979 | 111411 |
| incorporation of thymine into phi e dna during transfection of bacillus subtilis. | | 1979 | 111413 |
| conditional antifolate resistance in bacillus subtilis thya. | resistance to antifolates in bacillus subtilis strains results from the presence of an antifolate resistance mutation (afo). strains which are thya(+)afo are unconditionally resistant to antifolates. the conditional resistance of thya afo strains is hypothesized to be due to the thyb(+) gene product (thymidylate synthetase b) having a high k(m) for the folate substrate, thus leading to thymineless death in the presence of antifolates. an alternative model for conditional antifolate resistance wa ... | 1979 | 111614 |
| properties of glutamate dehydrogenase from bacillus subtilis. | | 1979 | 111677 |
| methods for limiting the action of sp3 dnaase and for the determination of the direction of hydrolysis of processive exonucleases. | the action of the exonuclease sp3 dnaase is inhibited by chemical modification of dna with the cation n-cyclohexyl-n'-beta-(4-methylmorpholinium)-ethylcarbodiimide (cme). the limited activity of the enzyme on cma-modified dna makes it possible to demonstrate that the enzyme also initiates its attack on polydeoxyribonucleotides at the 5'-termini. this was determined by the analysis of the products from the digestion of cme-modified dna containing labeled 5'-terminal phosphate groups. such procedu ... | 1979 | 111714 |
| the phosphoenolpyruvate : methyl-alpha-d-glucoside phosphotransferase system in bacillus subtilis marburg : kinetic studies of enzyme ii and evidence for a phosphoryl enzyme ii intermediate. | the enzyme ii complex catalyzing the phosphoryl transfer from p-hpr to sugar in the inducible methyl-alpha-d-glucoside : phosphotransferase system in bacillus subtilis acts according to a ping-pong mechanism, implying a phosphorylated enzyme ii intermediate. this result is supported by the demonstration of a specific transphosphorylation between [14c] alphamg and glucose-6-phosphate in the presence of an induced enzyme ii preparation. | 1978 | 111719 |
| rotor speed dependent sedimentation of circular and linear dna. | the sedimentation rate of large, linear dna molecules has been shown to be rotor speed dependent (rubenstein and leighton, biophys. chem. 1 (1974)). in this communication we report the first studies designed to measure the rotor speed effect with a homogenous, linear viral dna larger than bacteriophage t2 dna. we also report the first studies using a homogenous, circular episomal dna of known molecular weight. for this circular dna a small rotor speed effect, previously unsuspected, was discover ... | 1979 | 111726 |
| serum kinetics of doxycycline polyphosphate in dogs. | serum kinetics of doxycycline polyphosphate (dpp) have been studied in dogs after oral administration of 10 mg.kg-1 by measurement of total serum concentration (ct) of tetracycline derivatives by a chemical assay and active concentration (ca) by a microbiological method. kinetics have been studied using a one compartment open model with absorption by oral route. dpp is rapidly absorbed, the peak serum level is reached three hours after absorption and slowly eliminated (elimination half-life = 12 ... | 1979 | 111939 |
| [riboflavin biosynthesis operon of bacillus subtilis. effect of genotype on guanosine-5-triphosphate cyclohydrolase synthesis]. | | 1979 | 112004 |
| purification and properties of bacillus subtilis inositol dehydrogenase. | inositol 2-dehydrogenase (ec 1.1.1.18) activity appears during growth of bacillus subtilis (strain 60015) in nutrient sporulation medium. its synthesis is induced by myo-inositol and repressed by d-glucose. the enzyme has an apparent molecular weight of 155,000 to 160,000 as determined by sucrose density gradient centrifugation, and it is comprised of four subunits, each having a molecular weight of 39,000 as determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. the isoelectri ... | 1979 | 112095 |
| ribosomal protein alterations in thiostrepton- and micrococcin-resistant mutants of bacillus subtilis. | ribosomal proteins of parental thiostrepton- and micrococcin-sensitive bacillus subtilis cysa14 and thiostrepton-and micrococcin-resistant mutants were compared. several electrophoretic and immunochemical techniques showed unambiguously that bs-l11 was not present on 50 s ribosomal subunits from the six thiostrepton-resistant mutants. protein bs-l11 reappeared in all six revertants from thiostrepton resistance to thiostrepton sensitivity. no definitive protein alteration could be ascribed to the ... | 1979 | 112097 |
| membrane-bound and soluble extracellular alpha-amylase from bacillus subtilis. | extracellular alpha-amylase was purified to homogeneity from a marburg strain of bacillus subtilis. the enzyme is a single polypeptide chain of molecular weight approximately 67,000. its nh2-terminal amino acid sequence is leu-thr-ala-pro-ser-ile-lys. a membrane-derived alpha-amylase was solubilizing from membrane vesicles by treatment with triton x-100 and was highly purified by chromatography on an anti-alpha-amylase-protein a-sepharose column. membrane-derived alpha-amylase was indistinguisha ... | 1979 | 112102 |
| genetic analysis of a class of polymyxin resistant partial revertants of stage o sporulation mutants of bacillus subtilis: map of the chromosome region near the origin of replication. | | 1979 | 112355 |
| [alpha-amylase biosynthesis by variants of bacillus subtilis]. | | 1979 | 112362 |
| in vitro genetic labeling of bacillus subtilis cryptic plasmid phv400. | | 1979 | 112601 |
| [a new repair pathway which involves direct excision of bases from dna (author's transl)]. | | 1979 | 112647 |
| biological indicators for the control of ethylene oxide sterilization. | a new biological indicator has been developed for the control of ethylene oxide sterilization, particularly for large scale sterilization of disposable medical equipment. the aim has been to provide the new indicator with the same resistance to the combined effect of ethylene oxide and water vapour as the biological indicator referred to by the health authorities in scandinavia. the reference indicator contains spores of a danish test strain, bacillus subtilis, in sand. the new one contains spo ... | 1979 | 112834 |
| pleiotropic control mutations affecting the sporulation of bacillus subtilis. | mutations affecting quantitatively the production of the sporulation-associated extracellular alkaline protease were isolated and characterized. they fall into at least five genes, three of which, scoa, b and c, were mapped in the argc-metc region. the pleiotropic effects of these mutations concern several or all of the following: rate and timing of protease production, synthesis of alkaline phosphatase, time-course of spore formation. electron microscopic evidence indicates delayed switch from ... | 1978 | 112899 |
| rna processing and the intervening sequence problem. | | 1979 | 112912 |
| growth of thymine auxotrophs on selected analogs. | | 1979 | 113010 |
| adsorption of bacillus subtilis bacteriophage pbs 1. | bacteriophage pbs 1 adsorbs initially on the flagella of its host, bacillus subtilis (stage i). the phage can adsorb to both active and inactive flagella. flagellar attachment is nonspecific as pbs 1 was shown to attach to the flagella of bacillus species other than the normal host b. subtilis. the phage particle then quickly moves down the length of the flagellum to its base, the final adsorption site. flagellar motion is required for flagellar base attachment (stage ii). after proper attachmen ... | 1978 | 113063 |
| the action of a bacterial amylase on some modified substrates. | | 1979 | 113099 |
| uracil incorporation into nascent dna of bacillus subtilis and escherichia coli. | | 1979 | 113168 |
| organization of the replication-origin region of the bacillus subtilis chromosome. | | 1979 | 113169 |
| a physical map of the genome of temperate phage phi 3t. | a physical map of the genome of bacillus subtilis bacteriophage phi 3t was constructed by ordering the fragments produced by cleavage of phi 3t dna with restriction endonucleases avaii (2 fragments), bgli (2 fragments), smai (3 fragments), bamhi (6 fragments), sali (7 fragments), avai (7 fragments), saci (12 fragments), psti (14 fragments), and bglii (26 fragments). two techniques were used to order the fragments: (1) sets of previously ordered restriction fragments were isolated and redigested ... | 1979 | 113290 |
| insertional inactivation of trpc in cloned bacillus trp segments: evidence for a polar effect on trpf. | plasmid pub110 was previously used as a vector to clone fragments of deoxyribonucleic acid that complement the trpc2 mutation in bacillus subtilis from endonuclease ecori digested b. licheniformis, b. pumilus, and b. subtilis cellular deoxyribonucleic acid. each of several such trp plasmids was subsequently shown to contain a segment of the trp gene cluster on the basis of genetic complementing activity. in the present study, analysis of the trp enzyme levels in b. subtilis harboring the constru ... | 1979 | 113380 |
| rece4-independent recombination between homologous deoxyribonucleic acid segments of bacillus subtilis plasmids. | a plasmid (pls104) carrying a tandem repetition of the leu region of the bacillus subtilis chromosome arose spontaneously from pls103, which carried a single copy of the leu region. plasmid preparations from strains harboring pls104 also contained the original plasmid, pls103, and, in some preparations, plasmids carrying three or four repetitions of the leu region. these plasmids were shown to be generated by recombination between homologous deoxyribonucleic acid (dna) segments in the tandemly r ... | 1979 | 113386 |
| membrane enrichment of genetic markers close to the origin and terminus during the deoxyribonucleic acid replication cycle in bacillus subtilis. | a temperature-sensitive bacillus subtilis initiation mutant was used to achieve one cycle of synchronized deoxyribonucleic acid (dna) replication. markers near the origin of replication and the terminus were assayed for association with the cell membrane at intervals during the dna replication cycle. dna near the origin and terminus was found to be enriched in the membrane fraction throughout the dna replication cycle. the magnitude of membrane enrichment or origin and terminus markers varied co ... | 1979 | 113389 |
| purification of bacillus subtilis rna polymerase with heparin-agarose. in vitro transcription of phi 29 dna. | we have devised a new procedure for the purification of highly active preparations of bacillus subtilis rna polymerase holoenzyme. a column of heparin-agarose a-15m is used to rapidly and quantitatively adsorb rna polymerase from the initial crude extract fraction. this affinity procedure obviates the necessity of including nucleic acid precipitation or partitioning steps and allows for rapid separation of rna polymerase from proteolytic activity. the enzyme is further purified by preparative gl ... | 1979 | 113409 |
| intrastrand self-complementary sequences in bacillus subtilis dna. | intrastrand self-complementary sequences have been isolated from the dna of bacillus subtilis by hydroxyapatite (ha) chromatography following thermal renaturation of strands separated by chromatography on methylated albumin kieselguhr (mak). the instrastrand structures derived from the mak h strand (ha hii) were biologically active showing transforming activity for a wide variety of markers, as well as hybridization to both pulse-labelled and ribosomal rna. removal of regions of single-strand dn ... | 1979 | 113482 |
| threonine inhibition of growth of bacillus subtilis: positive selection for isoleucine auxotrophy. | | 1979 | 113483 |
| the effect of transition metal ions on the resistance of bacterial spores to hydrogen peroxide and to heat. | the presence of 10 microm-cu2+ increased the lethal effect of hydrogen peroxide on spores of clostridium bifermentans but not on those of clostridium sporogenes pa 3679, clostridium perfringens, bacillus cereus or bacillus subtilis var. niger. cu2+ at 100 mum also increased the lethal effect of heat on spores of c. bifermentans but not on those of b. sutilis var. niger. the rate and extent of cu2+ uptake by spores of c. bifermentans and b. subtilis var. niger were similar, but examination of uns ... | 1979 | 113488 |
| genetic instability of sporulation-associated characters in a bacillus subtilis mutant: analysis of the segregation pattern and genetic studies. | a bacillus subtilis mutant which formed dark-brown 'medusa' (m) colonies was obtained. it sporulated at a high frequency, overproduced extracellular protease during sporulation and possessed a high genetic instability with a complex segregation pattern. segregation was maintained after repeated re-isolation of single m colonies. the major wild-type-like class of segregants (b) was stable, sporulated normally and produced normal amounts of protease. occasionally segregants were obtained which pro ... | 1979 | 113490 |
| genetic instability of sporulation-associated characters in a bacillus subtilis mutant: relationship between sporulation, segregation and the synthesis of extracellular enzymes (kinetic studies). | in the genetically unstable, protease-overproducing 'medusa (m) strains of bacillus subtilis, segregation of stable, wild-type-like b cells occurred mainly during sporulation. after the end of the exponential growth phase, a small fraction of m cells sporulated quickly and formed m spores, while the majority of the cells, after a 'critical period', gave rise to b segregants which sporulated after a delay. segregation occurred without cell division. delayed sporulation, segregation and protease o ... | 1979 | 113491 |
| [existence of cysteic acid and taurine in sporulating cells of bacillus subtilis nrrl b558 (author's transl)]. | | 1979 | 113603 |
| the nature of transcription selectivity of bacteriophage spo1-modified rna polymerase. | escherichia coli and bacillus subtilis rna polymerase have almost identical transcription specificities on bacteriophage spo1 dna when assayed in a coupled transcription-translation cell free system. spo1-modified b. subtilis rna polymerase has altered transcription specificity. it is shown that rifampicin-inhibited e. coli rna polymerase can completely block transcription of spo1 dna by rifampicin resistant b. subtilis enzyme, whereas it has no effect on transcription by spo1-modified b. subtil ... | 1979 | 113644 |
| sigma factor is not released during transcription in bacillus subtilis. | the relationship between sigma (sigma) and delta (delta) factors of bacillus subtilis rna polymerase has been analyzed during initiation of rna synthesis. when core enzyme (e) containing delta factor (e delta) binds to dna, the delta factor is released with the formation of an e-dna complex. the addition of sigma to the e-dna complex results in the formation of a stable e sigma-dna complex which can synthesize rna upon addition of nucleoside triphosphates. sigma factor, significantly, is not rel ... | 1979 | 113645 |
| different specific activities of the monomeric and oligomeric forms of plasmid dna in transformation of b. subtilis and e. coli. | (1) the low residual transforming activity in preparations of monomeric, supercoiled, circular (ccc) forms of the plasmids pc194 and phv14 could be attributed to the presence in such isolates of a small number of contaminating multimeric molecules. (2) e. coli derived preparations of phv14, as in vitro recombinant plasmid capable of replication in both e. coli and b. subtilis, contain oligomeric forms of plasmid dna in addition to the prevalent monomeric ccc form. the specific transforming activ ... | 1979 | 113646 |
| mapping the uroporphyrinogen iii cosynthase locus in bacillus subtilis. | the gene hemd taking part in the formation of uroporphyrinogen iii from porphobilinogen was mapped by two- and three-factor transduction crosses in bacillus subtilis. this gene codes uroporphyrinogen iii cosynthase. the gene hemd is linked to the hema locus and is located between the hema and phea loci. | 1979 | 113648 |
| [effect of uv radiation on the dna-membrane complex of bacillus subtilis]. | the influence of uv-light on dna-membrane complex (dmc) of bacillus subtilis was studied. an increased dna content in dmc for strains 168 and rec a-, and a degradation of dmc for strain pola- have been registrated. the increase in dna in dmc of the two former strains is inhibited by caffeine to be correlated with changes in protein content in dmc, determined by a radioactive label, but not with lipid content. thus, the association of dna with the membrane is mediated by proteins. dna increasing ... | 1979 | 113919 |
| [studies to investigate the ecological importance of the mass development of hydrodicyton reticulatum in infiltration basins for drinking water. ii. localization of the active components with the aid of thin-layer chromatography and bioassay detection (author's transl)]. | thin-layer chromatogramms made from ethanol extracts of the green fresh-water alga h. reticulatum were tested by bioassay detection against several strains of bac. subtilis. the chromatogramms were overlayed with agar, seeded with the test strains. the production of two growth-inhibiting zones could be demonstrated (fig. 1). one of them seems to be correlated with chlorophyll derivatives. the second zone--larger than the first one--was formed by a fatty acid fraction which was more active in cel ... | 1979 | 113957 |