| secondary calcium-binding parameter of bacillus amyloliquefaciens alpha-amylase obtained from inhibition kinetics. | calcium is required for the stabilization of alpha-amylase because of primary binding (essential binding), but has been shown to inhibit hydrolytic catalysis due to secondary binding at the catalytic site in the enzyme. the role of calcium in the hydrolysis of soluble amylose by bacillus amyloliquefaciens alpha-amylase was characterized using the equilibrium dissociation constant (k(m)) and k(cat) for the hydrolytic catalysis. the enzymatic hydrolysis was inhibited by a relatively high concentra ... | 2003 | 16233519 |
| hyper expression of kojibiose phosphorylase gene and trehalose phosphorylase gene from thermoanaerobacter brockii atcc35047 in bacillus subtilis and selaginose synthesis utilizing two phosphorylases. | the kojibiose phosphorylase (kp) gene and trehalose phosphorylase (tp) gene from thermoanaerobacter brockii atcc35047 were intracellularly hyper-expressed under the control of the bacillus amyloliquefaciens alpha-amylase promoter in bacillus subtilis. the production yields were estimated to be 2.1 g of kp and 4.9 g of tp per liter of medium. selaginose, non-reducing trisaccharide, was synthesized from trehalose utilizing the recombinant kp and tp from b. subtilis. selaginose was not hydrolyzed b ... | 2005 | 16243288 |
| [pcr based identification of strains of endophytic ecotype of bacillus amyloliquefaciens]. | a search for genetic markers of an endophytic ecotype of bacillus amyloliquefaciens was performed by the subtractive hybridization technique. the isolated fragments specificity was checked by dna-dna hybridization. the sequence of a dna locus exhibiting the highest taxon specificity has manifested a 76% identity to the gene mtla from bacillus subtilis 168 genome. a set of discriminative primers has been derived from this sequence which could be used for the fast and reliable identification of is ... | 2005 | 16250231 |
| a new vector for controllable expression of an anti-her2/neu mini-antibody-barnase fusion protein in hek 293t cells. | tumor-targeted vectors with controllable expression of therapeutic genes and specific antitumor antibodies are promising tools for the reduction of malignant tumors. here we describe a new plasmid for the eukaryotic expression of an anti-her2/neu mini-antibody-barnase fusion protein (4d5 scfv-barnase-his(5)) with an nh(2)-terminal leader peptide. the 4d5 scfv-barnase-his(5) gene was placed downstream of the tetracycline responsive-element minimal promoter in the vector using the tet-off gene-exp ... | 2006 | 16300908 |
| corrected version distribution of heterogeneous and homologous plasmids in bacillus spp. | a total of 75 strains (including 5 reference strains) of bacillus amyloliquefaciens, b. cereus, b. circulans, b. licheniformis, b. megaterium, b. pumilus, b. sphaericus, b. subtilis, and b. thuringiensis and 36 species-unidentified bacillus strains were surveyed for plasmids by cesium chloride-ethidium bromide equilibrium centrifugation of cell lysates in a study of antibiotic resistance in host cells. of the 111 strains, 13 (including 3 reference strains) were found to harbor plasmids, and 5 of ... | 1983 | 16346439 |
| protoplast transformation in coryneform bacteria and introduction of an alpha-amylase gene from bacillus amyloliquefaciens into brevibacterium lactofermentum. | [this corrects the article on p. 638 in vol. 51.]. | 1986 | 16347093 |
| characterization of a novel pepf-like oligopeptidase secreted by bacillus amyloliquefaciens 23-7a. | an oligopeptidase from bacillus amyloliquefaciens 23-7a was characterized along with its biochemical activities and structural gene. the protein's amino acid sequence and enzymatic activities were similar to those of other bacterial pepfs, which belong to metallopeptidase family m3. while most bacterial pepfs are cytoplasmic endopeptidases, the identified pepfba oligopeptidase is a secreted protein and may facilitate the process of sporulation. | 2006 | 16391147 |
| thermostabilization of bacillus amyloliquefaciens alpha-amylase by chemical cross-linking. | chemical cross-linking of a mesophilic alpha-amylase from bacillus amyloliquefaciens (baa) was carried out. intra-molecular cross-links between lysine residues upon treatment of the enzyme with ethylene glycol bis(succinic acid n-hydroxy succinimide ester) resulted in enhancement of thermostability as indicated by irreversible thermoinactivation experiments. enhancement of thermostability coincided with a dramatic protection against aggregation, combined with a decrease in surface hydrophobicity ... | 2006 | 16446001 |
| influence of bacillus spp. isolated from maize agroecosystem on growth and aflatoxin b(1) production by aspergillus section flavi. | a total of 59 bacteria of the bacillus genus were isolated from different components of a maize agroecosystem and their antifungal activity against aspergillus section flavi was evaluated. thirty-three and 46% of these bacteria were able to inhibit aspergillus flavus link and a. parasiticus speare respectively at water activity (a(w)) 0.982; however, when a(w) was 0.955, these percentages were decreased and only three isolates were able to inhibit aspergillus section flavi. the majority of bacil ... | 2006 | 16475221 |
| comparison of the molten globule states of thermophilic and mesophilic alpha-amylases. | in recent years great interest has been generated in the process of protein folding, and the formation of intermediates during the folding process has been proven with new experimental strategies. in the present work, we have examined the molten globule state of bacillus licheniformis alpha-amylase (bla) by intrinsic fluorescence and circular dichroism spectra, 1-anilino naphthalene-8-sulfonate (ans) binding and proteolytic digestion by pepsin, for comparison to its mesophilic counterpart, bacil ... | 2006 | 16516372 |
| [identification and characterization of a bacillus amyloliquefaciens with high antifungal activity]. | plant disease can cause serious crop losses, and chemical control of disease is costly both to the environment and to the farmer. some microorganism can produce the substance which has the preventing and exterminating functions to plant pathogens. these substances are valid to plant pathogens with only lower concentration, in addition the substances do not remain in soil and crops without being decomposed. if composization is performed with the microorganism, or the microorganism is mixed into c ... | 2006 | 16579456 |
| [dual promoters enhance heterologous enzyme production from bacterial phage based recombinant bacillus subtilis]. | the effect of dual promoters on recombinant protein production from bacterial phage based bacillus subtilis expression system was investigated. alpha amylase (from bacillus amyloliquefaciens) and penicillin acylase (from bacillus megaterium) were selected as the indicating enzymes. both the promoterless genes and the promoter-bearing genes were isolated through pcr amplification with properly designed primers, and were inserted into plasmid psg703 that contains the lacz-cat expression cartridge. ... | 2006 | 16607942 |
| effects of degu32(hy), degqa and degr pleiotropic regulatory genes on the growth and protease fermentation of bacillus subtilis ki-2-132. | effects of degu32 (hy), degr genes from bacillus subtilis 168 and degqa gene from bacillus amyloliquefaciens on bacillus subtilis ki-2-132 cell growth, sporulation and protease fermentation were investigated by introducing these genes into b. subtilis ki-2-132 chromosome and/or cytoplasm. although the genes come from different species and strains, they showed pleiotropic effects in b. subtilis ki-2-132. b. subtilis ki-2-132degu32 (hy) showed increased protease production, and when cooperating wi ... | 2006 | 16625836 |
| combined pressure-thermal inactivation kinetics of bacillus amyloliquefaciens spores in egg patty mince. | bacillus amyloliquefaciens is a potential surrogate for clostridium botulinum in validation studies involving bacterial spore inactivation by pressure-assisted thermal processing. spores of b. amyloliquefaciens fad 82 were inoculated into egg patty mince (approximately 1.4 x 10(8) spores per g), and the product was treated with combinations of pressure (0.1 to 700 mpa) and heat (95 to 121 degrees c) in a custom-made high-pressure kinetic tester. the values for the inactivation kinetic parameter ... | 2006 | 16629029 |
| [brownian dynamics simulation of the association of bacillus amyloliquefaciens ribonuclease (barnase) and bacillus intermedius ribonuclease (binase) with barstar]. | a comparative study of the association of two ribonucleases, barnase and binase, with the polypeptide inhibitor barstar has been performed by the brownian dynamics simulation method. it was shown that the method adequately reproduced the dependence of the association rate on ph and ionic strength of solution and the influence of mutations of some ribonuclease amino acids. two types of energetically favorable complexes of binase-barstar encounter were determined. in the type i complex, the amino ... | 2006 | 16637328 |
| mitochondrial dna from oenothera berteriana: purification and properties. | mitochondrial dna (mt-dna) from oenothera berteriana tissue culture cells was isolated and characterized with respect to buoyant density in cscl, melting point, contour length, and restriction fragments.because of the rather long purification procedure very few molecules retained their circularity. only one distinct size class of molecules with a length of 100 kilobases was found. restriction fragments were obtained with the enzymes, restrictionendonuclease i from serratia marcescens, restrictio ... | 1980 | 16661361 |
| high-pressure-mediated survival of clostridium botulinum and bacillus amyloliquefaciens endospores at high temperature. | endospores of proteolytic type b clostridium botulinum tmw 2.357 and bacillus amyloliquefaciens tmw 2.479 are currently described as the most high-pressure-resistant bacterial spores relevant to food intoxication and spoilage in combined pressure-temperature applications. the effects of combined pressure (0.1 to 1,400 mpa) and temperature (70 to 120 degrees c) treatments were determined for these spores. a process employing isothermal holding times was established to distinguish pressure from te ... | 2006 | 16672493 |
| detection of antibiotic-related genes from bacterial biocontrol agents with polymerase chain reaction. | pseudomonas chlororaphis pa23, pseudomonas spp. strain df41, and bacillus amyloliquefaciens bs6 consistently inhibit infection of canola petals by sclerotinia sclerotiorum in both greenhouse and field experiments. bacillus thuringiensis bs8, bacillus cereus l, and bacillus mycoides s have shown significant inhibition against s. sclerotiorum on plate assays. the presence of antibiotic biosynthetic or self-resistance genes in these strains was investigated with polymerase chain reaction and, in on ... | 2006 | 16699573 |
| structural and functional characterization of three polyketide synthase gene clusters in bacillus amyloliquefaciens fzb 42. | although bacterial polyketides are of considerable biomedical interest, the molecular biology of polyketide biosynthesis in bacillus spp., one of the richest bacterial sources of bioactive natural products, remains largely unexplored. here we assign for the first time complete polyketide synthase (pks) gene clusters to bacillus antibiotics. three giant modular pks systems of the trans-acyltransferase type were identified in bacillus amyloliquefaciens fzb 42. one of them, pks1, is an ortholog of ... | 2006 | 16707694 |
| free energies of protein-protein association determined by electrospray ionization mass spectrometry correlate accurately with values obtained by solution methods. | the advantages of electrospray ionization mass spectrometry (esims) to measure relative solution-phase affinities of tightly bound protein-protein complexes are demonstrated with selected variants of the bacillus amyloliquefaciens protein barstar (b*) and the rnaase barnase (bn), which form protein-protein complexes with a range of picomolar to nanomolar dissociation constants. a novel chemical annealing procedure rapidly establishes equilibrium in solutions containing competing b* variants with ... | 2006 | 16731980 |
| production of recombinant rnase ba and its application in downstream processing of plasmid dna for pharmaceutical use. | the demand for new strategies in downstream processing of biopharmaceutical plasmid dna has increased in response to the importance of nucleic acids as active pharmaceutical ingredients (api) in gene therapy and genetic vaccination. led by the problematic usage of animal-derived proteins for producing reagents of clinical applications, we present an opportunity of removing rna prior to chromatographic steps by using a recombinant rnase ba (barnase of bacillus amyloliquefaciens) as an alternative ... | 2006 | 16739957 |
| significant differences in the activities of alpha-amylases in the absence and presence of polyethylene glycol assayed on eight starches solubilized by two methods. | starch is a reserve chemical source of the energy of the sun found in plants as a water-insoluble granule that differs in their chemical and physical properties, depending on the source. the granules can be solubilized by heating in water or by treatment with various reagents, such as 1m naoh. alpha-amylases are widely distributed enzymes that initiate the hydrolysis of starch into low molecular weight maltodextrins. we recently found that the activities of a single alpha-amylase on two differen ... | 2006 | 16762330 |
| aminotryptophan-containing barstar: structure--function tradeoff in protein design and engineering with an expanded genetic code. | the indole ring of the canonical amino acid tryptophan (trp) possesses distinguished features, such as sterical bulk, hydrophobicity and the nitrogen atom which is capable of acting as a hydrogen bond donor. the introduction of an amino group into the indole moiety of trp yields the structural analogs 4-aminotryptophan ((4-nh(2))trp) and 5-aminotryptophan ((5-nh(2))trp). their hydrophobicity and spectral properties are substantially different when compared to those of trp. they resemble the puri ... | 2006 | 16782415 |
| comparison of the wild-type alpha-amylase and its variant enzymes in bacillus amyloliquefaciens in activity and thermal stability, and insights into engineering the thermal stability of bacillus alpha-amylase. | the starch hydrolysis activity and thermal stability of bacillus amyloliquefaciens alpha-amylase (wild-type enzyme or wt) and its variant enzymes, designated as m77, m111, and 21b, were compared. all have an optimal ph at around 6, as well as almost the same reaction rates and km and kcat values. the optimal temperature in the absence of ca2+ ions is 60 degrees c for wt and m77 and 40 degrees c for m111 and 21b. those of m111 and 21b rose to 50-60 degrees c upon the addition of 5 mm cacl2, while ... | 2006 | 16788051 |
| lysogenic conversion in bacillus amyloliquefaciens h affecting viral adsorption. | lysogenization of bacillus amyloliquefaciens h with bacteriophage pk renders the bacteria resistant to superinfection with bacteriophage of pk type. | 1969 | 16789108 |
| antibiosis by bacillus amyloliquefaciens ribonuclease barnase expressed in escherichia coli against symbiotic and endophytic nitrogen-fixing bacteria. | a modified antibiosis assay was used to evaluate growth inhibition of symbiotic and endophytic bacteria by e. coli strains producing bacillus amyloliquefaciens ribonuclease, barnase. inhibition zones were only observed when the assays were performed in minimal medium agar. however, bacterial growth inhibition was not detected when using rich medium or susceptible strains expressing the ribonuclease inhibitor protein, barstar. our results suggest that barnase may act as a broad range bacteriocin. ... | 2006 | 16797765 |
| construction and use of retroviral vectors encoding the toxic gene barnase. | suicide genes for negative selection of cells have been powerful tools in somatic cell genetic studies and in gene therapy. here we report on the construction, characterization, and utilization of retroviral vectors encoding barnase, a ribonuclease from bacillus amyloliquefaciens, expression of which results in apoptosis of transduced mammalian cells. high-titer viral vector production was enabled by expression of an inhibitor of barnase (barstar) in transfected cells generating murine leukemia ... | 2006 | 16814610 |
| characterization of a bacteriocin-like substance produced by bacillus amyloliquefaciens isolated from the brazilian atlantic forest. | a bacillus strain producing a bacteriocin-like substance was characterized by biochemical profiling and 16s rdna sequencing. phylogenetic analysis indicated that the strain has high sequence similarity with bacillus amyloliquefaciens. the antimicrobial substance was inhibitory to pathogenic and food-spoilage bacteria, such as listeria monocytogenes, bacillus cereus, serratia marcescens, and pasteurella haemolytica. it was stable over a wide temperature range, but lost activity when the temperatu ... | 2006 | 16835841 |
| rts, a rice anther-specific gene is required for male fertility and its promoter sequence directs tissue-specific gene expression in different plant species. | a tapetum-specific gene, rts, has been isolated by differential screening of a cdna library from rice panicles. rts is a unique gene in the rice genome. rna blot analysis and in situ hybridization indicates that this gene is predominantly expressed in the anther's tapetum during meiosis and disappears before anthesis. rts has no introns and encodes a putative polypeptide of 94 amino acids with a hydrophobic n-terminal region. the nucleotide and deduced amino acid sequence of the gene do not show ... | 2006 | 16897470 |
| construction and characterization of a bifunctional fusion enzyme of bacillus-sourced beta-glucanase and xylanase expressed in escherichia coli. | a chimeric gene, glu-xyl, encoding bacillus amyloliquefaciens glucanase (glu, 24.4 kda) and bacillus subtilis xylanase (xyl, 21.2 kda), was constructed via end-to-end fusion and expressed successfully in escherichia coli. the purified fusion protein (46.1 kda) exhibited both glucanase and xylanase activities. compared with parental enzymes, the glu moiety was characterized by kinetic parameters of decreased k(m) (0.66-fold) and increased k(cat) (2.75-fold), whereas the xyl moiety had an increase ... | 2006 | 16907724 |
| construction of an arxula adeninivorans host-vector system based on trp1 complementation. | a host/vector expression system based on an arxula adeninivorans delta atrp1 gene disruption mutant has been constructed. for this purpose the atrp1 gene encoding a phosphoribosyl anthranilate isomerase was isolated from the yeast a. adeninivorans and its genome locus was characterized. the delta atrp1 mutant was generated applying an amplified dna fragment containing the aleu2m gene flanked by atrp1 gene sequences of some 750 bp. the generated auxotrophic host strain was transformed with the pl ... | 2007 | 16934902 |
| heterologous protein secretion in lactococcus lactis is enhanced by the bacillus subtilis chaperone-like protein prsa. | the bacillus subtilis lipoprotein prsa enhances the yield of several homologous and heterologous exported proteins in b. subtilis by being involved in the posttranslocational stage of the secretion process. in this work, we have studied the effect of b. subtilis prsa on the secretion of bacillus amyloliquefaciens alpha-amylase (amyq), a target protein for prsa, and bacillus licheniformis penicillinase (penp) a nontarget protein for prsa, in lactococcus lactis. two compatible plasmids were constr ... | 2006 | 16944130 |
| dual role of the phop approximately p response regulator: bacillus amyloliquefaciens fzb45 phytase gene transcription is directed by positive and negative interactions with the phyc promoter. | several bacillus strains secrete phytase, an enzyme catalyzing dephosphorylation of myo-inositol hexakisphosphate (phytate). we identified the phyc (phytase) gene from environmental bacillus amyloliquefaciens fzb45 as a member of the phosphate starvation-inducible phopr regulon. in vivo and in vitro assays revealed that phop approximately p is essential for phyc transcription. the transcriptional start site was identified downstream of a sigmaa-like promoter region located 27 bp upstream of the ... | 2006 | 16980498 |
| yak milk casein as a functional ingredient: preparation and identification of angiotensin-i-converting enzyme inhibitory peptides. | yak milk casein derived from qula, a traditional tibetan acid curd cheese, was hydrolyzed by six commercially available proteases (trypsin, pepsin, alcalase, flavourzyme, papain and neutrase). these hydrolysates were assayed for their inhibitory activity of angiotensin-i-converting enzyme (ace). the hydrolysates obtained by neutrase from bacillus amyloliquefaciens showed the highest ace inhibitory activity. the ic50 value of neutrase-hydrolysate was 0.38 mg/ml. the hydrolysate obtained by neutra ... | 2007 | 16987434 |
| inhibitory effects of spice essential oils on the growth of bacillus species. | a series of essential oils of 11 turkish plant spices [black thyme, cumin, fennel (sweet), laurel, marjoram, mint, oregano, pickling herb, sage, savory, and thyme], used in foods mainly for their flavor, aromas, and preservation, in herbal tea, in alternative medicines, and in natural therapies, were screened for antibacterial effects at 1:50, 1:100, 1:250, and 1:500 dilutions by the paper disc diffusion method against six bacillus species (bacillus amyloliquefaciens atcc 3842, bacillus brevis f ... | 2006 | 17004909 |
| small rolling circle plasmids in bacillus subtilis and related species: organization, distribution, and their possible role in host physiology. | bacillus subtilis and related species (bacillus licheniformis, bacillus pumilus, bacillus amyloliquefaciens, and bacillus mojavensis) represent a group of bacteria largely studied and widely employed by industry. small rolling circle replicating plasmids of this group of bacteria have been intensively studied as they represent a convenient model for genetic research and for the construction of molecular tools for the genetic modification of their hosts. through the computational analysis of the ... | 2007 | 17064773 |
| formation of covalent beta-linked carbohydrate-enzyme intermediates during the reactions catalyzed by alpha-amylases. | porcine pancreatic and bacillus amyloliquefaciens alpha-amylases were examined for the formation of covalent carbohydrate intermediates during reaction. the enzymes were precipitated and denatured by adding 10 volumes of acetone. when these denatured enzymes were mixed with methyl alpha-6-[(3)h]-maltooligosaccharide glycosides and chromatographed on biogel p-2, no carbohydrate was found in the protein void volume peak. when the enzymes were added to the methyl alpha-6-[(3)h]-maltooligosaccharide ... | 2007 | 17123489 |
| determination of spore inactivation during thermal and pressure-assisted thermal processing using ft-ir spectroscopy. | the efficacy of microbial inactivation techniques is currently tested using time-consuming and labor-intensive plate count methods, which are the principal rate-limiting steps in developing inactivation kinetic parameters for alternative food processing technologies. fourier transform infrared (ft-ir) spectroscopy combined with multivariate analysis was used to quantify viable spores and identify some biochemical changes in samples treated by autoclaving, pressure-assisted thermal processing (pa ... | 2006 | 17177574 |
| protection of oilseed rape (brassica napus) toward fungal pathogens by strains of plant-associated bacillus amyloliquefaciens. | in this report, four bacillus strains were tested for effects on plant fitness and disease protection of oilseed rape (brassica napus). the strains belonged to newly discovered plant-associated bacillus amyloliquefaciens and a recently proposed species, bacillus endophyticus. the fungal pathogens tested represented different infection strategies and included alternaria brassicae, botrytis cinerea, leptosphaeria maculans, and verticillium longisporum. the b. amyloliquefaciens strains showed no or ... | 2007 | 17186140 |
| inactivation kinetics of selected aerobic and anaerobic bacterial spores by pressure-assisted thermal processing. | the combined pressure-thermal inactivation kinetics of spores from three strains of anaerobic (clostridium sporogenes, c. tyrobutylicum, and thermoanaerobacterium thermosaccharolyticum), and six strains of aerobic (bacillus amyloliquefaciens and b. sphaericus) bacteria were studied. spores of these bacteria were prepared in deionized water and treated in a custom-made kinetic tester over various pressure (0.1 and 700 mpa) and thermal (105 and 121 degrees c) combinations. survivor data were model ... | 2007 | 17196696 |
| intrinsic halotolerance of the psychrophilic alpha-amylase from pseudoalteromonas haloplanktis. | the halotolerance of a cold adapted alpha-amylase from the psychrophilic bacterium pseudoalteromonas haloplanktis (aha) was investigated. aha exhibited hydrolytic activity over a broad range of nacl concentrations (0.01-4.5 m). aha showed 28% increased activity in 0.5-2.0 m nacl compared to that in 0.01 m nacl. in contrast, the corresponding mesophilic (bacillus amyloliquefaciens) and thermostable (b. licheniformis) alpha-amylases showed a 39 and 46% decrease in activity respectively. even at 4. ... | 2007 | 17310272 |
| purification and characterization of cellulase produced by bacillus amyoliquefaciens dl-3 utilizing rice hull. | a microorganism hydrolyzing rice hull was isolated from soil and identified as bacillus amyloliquefaciens by analysis of 16s rdna and partial sequences of the gyra gene, and named as b. amyloliquefaciens dl-3. with the analysis of sds-page, the molecular weight of the purified cellulase was estimated to be 54kda. the purified cellulase hydrolyzed avicel, caboxymethylcellulose (cmc), cellobiose, beta-glucan and xylan, but not p-nitrophenyl-beta-d-glucopyranoside (pnpg). optimum temperature and ph ... | 2008 | 17320379 |
| amylosin from bacillus amyloliquefaciens, a k+ and na+ channel-forming toxic peptide containing a polyene structure. | bacillus amyloliquefaciens strains isolated from the indoor environment of moisture-damaged buildings produce a 1197 da toxin, named amylosin. nuclear magnetic resonance (nmr) data showed that amylosin contains a chromophoric polyene structure and the amino acids leucine/isoleucine, proline, aspartic acid/asparagine, glutamic acid/glutamine and tyrosine. a quantitation method for amylosin was developed using commercially available amphotericin b as a reference compound and a known concentration ... | 2007 | 17391722 |
| photoperiod regulates elicitation of growth promotion but not induced resistance by plant growth-promoting rhizobacteria. | for several years, we have noticed that plant growth-promoting rhizobacteria (pgpr), which consistently promote plant growth in greenhouse tests during spring, summer, and fall, fail to elicit plant growth promotion during the midwinter under ambient light conditions. this report tests the hypothesis that photoperiod regulates elicitation of growth promotion and induced systemic resistance (isr) by pgpr. a commercially available formulation of pgpr strains bacillus subtilis gb03 and bacillus amy ... | 2007 | 17496963 |
| purification and characterization of a subtilisin-like proteinases secreted in the stationary growth phase of bacillus amyloliquefaciens h2. | proteinases secreted during the early and late stationary phases have been isolated from the culture liquid of bacillus amyloliquefaciens h2 using cm-cellulose ion-exchange chromatography with subsequent fplc on a mono s column. considering the character of hydrolysis of specific chromogenic substrates and the type of inhibition, these enzymes were identified as subtilisin-like proteinases. the molecular weight of both proteinases is 29 kd. the proteolytic activity of the proteinases secreted du ... | 2007 | 17511612 |
| isolation and structural analysis of bamylocin a, novel lipopeptide from bacillus amyloliquefaciens lp03 having antagonistic and crude oil-emulsifying activity. | bacillus amyloliquefaciens strain lp03 isolated from soil, produced an antagonistic compound that strongly inhibited the growth of plant-pathogenic fungi and a lipopeptide biosurfactant. also, isolated strain lp03 had a marked crude oil-emulsifying activity as it developed a clear zone around the colony after incubation for 24 h at 37 degrees c. lp03 was identified as bacillus amyloliquefaciens by analysis of partial 16 s rrna gene and partial gyra gene sequence. the lipopeptide was purified by ... | 2007 | 17530228 |
| tryptophan-dependent production of indole-3-acetic acid (iaa) affects level of plant growth promotion by bacillus amyloliquefaciens fzb42. | phytohormone-like acting compounds previously have been suggested to be involved in the phytostimulatory action exerted by the plant-beneficial rhizobacterium bacillus amyloliquefaciens fzb42. analyses by high-performance liquid chromatography and gas chromatography-mass spectrometry performed with culture filtrates of fzb42 demonstrated the presence of indole-3-acetic acid (iaa), corroborating it as one of the pivotal plant-growth-promoting substances produced by this bacterium. in the presence ... | 2007 | 17555270 |
| nucleotide sequence, structural investigation and homology modeling studies of a ca2+-independent alpha-amylase with acidic ph-profile. | the novel alpha-amylase purified from locally isolated strain, bacillus sp. kr-8104, (kra) (enzyme microb technol; 2005; 36: 666-671) is active in a wide range of ph. the enzyme maximum activity is at ph 4.0 and it retains 90% of activity at ph 3.5. the irreversible thermoinactivation patterns of kra and the enzyme activity are not changed in the presence and absence of ca(2+) and edta. therefore, kra acts as a ca(2+)-independent enzyme. based on circular dichroism (cd) data from thermal unfoldi ... | 2007 | 17562282 |
| production and secretion stress caused by overexpression of heterologous alpha-amylase leads to inhibition of sporulation and a prolonged motile phase in bacillus subtilis. | transcriptome analysis was used to investigate the global stress response of the gram-positive bacterium bacillus subtilis caused by overproduction of the well-secreted amyq alpha-amylase from bacillus amyloliquefaciens. analyses of the control and overproducing strains were carried out at the end of exponential growth and in stationary phase, when protein secretion from b. subtilis is optimal. among the genes that showed increased expression were htra and htrb, which are part of the cssrs regul ... | 2007 | 17586671 |
| occupational sensitization to fungal enzymes used in animal feed industry. | industrial enzymes cause the increasing prevalence of occupational hypersensitivity. our objective was to study workers occupationally exposed to fungal enzymes in 2 animal feed factories to determine if the sensitization originated in the enzymes or was caused by the microorganism used to produce the enzymes. | 2007 | 17587859 |
| pathway of phytate dephosphorylation by beta-propeller phytases of different origins. | using a combination of high-performance ion chromatography analysis and kinetic studies, the pathway of myo-inositol hexakisphosphate dephosphorylation by the beta-propeller phytase of shewanella oneidensis was established, which was then compared with that of bacillus subtilis 168, bacillus amyloliquefaciens atcc 15841, and b. amyloliquefaciens 45 beta-propeller phytases. the data demonstrate that all of these beta-propeller phytases dephosphorylate myo-inositol hexakisphosphate in a stereospec ... | 2007 | 17612603 |
| a novel vector element providing multicopy vector integration in arxula adeninivorans. | an arxula adeninivorans vector element has been identified that provides multicopy integration in an atrp1 host strain. the element consists of the atrp1 selection marker fused to a newly generated truncated aleu2 promoter of 53 bp. in the described example eight copies of an amya expression vector encoding heterologous alpha-amylase from bacillus amyloliquefaciens are integrated in the genome of the recombinant strain instead of a single copy observed when using the atrp1 element with the compl ... | 2007 | 17655689 |
| comparative analysis of the complete genome sequence of the plant growth-promoting bacterium bacillus amyloliquefaciens fzb42. | bacillus amyloliquefaciens fzb42 is a gram-positive, plant-associated bacterium, which stimulates plant growth and produces secondary metabolites that suppress soil-borne plant pathogens. its 3,918-kb genome, containing an estimated 3,693 protein-coding sequences, lacks extended phage insertions, which occur ubiquitously in the closely related bacillus subtilis 168 genome. the b. amyloliquefaciens fzb42 genome reveals an unexpected potential to produce secondary metabolites, including the polyke ... | 2007 | 17704766 |
| response surface methodology for the optimization of alpha amylase production by bacillus amyloliquefaciens. | the aim of this work was to optimize the cultural and production parameters through the statistical approach for the synthesis of alpha amylase by bacillus amyloliquefaciens in submerged fermentation (smf) using a combination of wheat bran and groundnut oil cake (1:1) as the substrate. the process parameters influencing the enzyme production were identified using plackett-burman design. among the various variables screened, the substrate concentration, incubation period and cacl2 concentration w ... | 2008 | 17761415 |
| crystallization and preliminary x-ray characterization of the bacillus amyloliquefaciens ywro enzyme. | cb1954 is an anticancer prodrug that is currently in clinical trials coupled with the escherichia coli flavoenzyme nitroreductase (ntr) for use in directed-enzyme prodrug therapy (dept). the ntr enzyme is responsible for the conversion of the prodrug into a cytotoxic agent. the bifunctional alkylating agent produced by this bioactivation process leads to dna damage and death of cancer cells. recently, a novel flavoenzyme from bacillus amyloliquefaciens, ywro (bam ywro), was reported to be able t ... | 2007 | 17768344 |
| degu and ycze positively regulate the synthesis of bacillomycin d by bacillus amyloliquefaciens strain fzb42. | environmental strain bacillus amyloliquefaciens fzb42 differs from the domesticated model organism of the same genus, bacillus subtilis 168, in its ability to promote plant growth and suppress plant-pathogenic organisms present in the rhizosphere. this behavior is exerted mainly through the production of several nonribosomal cyclic lipopeptides and polyketides, which exhibit a broad range of action against phytopathogenic bacteria, fungi, and nematodes. here, we provide evidence that the synthes ... | 2007 | 17827323 |
| macrolactin is the polyketide biosynthesis product of the pks2 cluster of bacillus amyloliquefaciens fzb42. | in the genome of bacillus amyloliquefaciens fzb42, three operons pks1, pks2, and pks3 were identified which encode the biosynthesis of polyketides. pks1 and pks3 have been attributed to the production of bacillaene and difficidin/oxydifficidin, respectively, while the pks2 product remained hitherto unknown. mass spectrometric analysis of the culture filtrates of the wild-type b. amyloliquefaciens fzb42 and mutants revealed pks2-specific metabolites. by combination of the mass spectrometric and u ... | 2007 | 17844999 |
| molecular and biochemical detection of fengycin- and bacillomycin d-producing bacillus spp., antagonistic to fungal pathogens of canola and wheat. | bacillus species are well known for their ability to control plant diseases through various mechanisms, including the production of secondary metabolites. bacillus subtilis dfh08, an antagonist of fusarium graminearum, and other bacillus spp. that are antagonists of common fungal pathogens of canola were screened for peptide synthetase biosynthetic genes of fengycin and bacillomycin d. specific polymerase chain reaction (pcr) primers identified b. subtilis strains dfh08 and 49 for the presence o ... | 2007 | 17898845 |
| monitoring biochemical changes in bacterial spore during thermal and pressure-assisted thermal processing using ft-ir spectroscopy. | pressure-assisted thermal processing (patp) is being widely investigated for processing low acid foods. however, its microbial safety has not been well established and the mechanism of inactivation of pathogens and spores is not well understood. fourier transform infrared (ft-ir) spectroscopy was used to study some of the biochemical changes in bacterial spores occurring during patp and thermal processing (tp). spore suspensions (approximately 10(9) cfu/ml of water) of clostridium tyrobutyricum, ... | 2007 | 17907780 |
| application of arabidopsis agamous second intron for the engineered ablation of flower development in transgenic tobacco. | to explore a new approach to generating reproductive sterility in transgenic plants, the barnase gene from bacillus amyloliquefaciens was placed under the control of an 1853-bp nucleotide sequence from the 3'end of the second intron of arabidopsis agamous and camv 35s (-60) minimal promoter [ag-i-35s (-60)::barnase], and was introduced into tobacco through transformation mediated by agrobacterium tumefaciens. all ag-i-35s (-60)::barnase transgenic plants showed normal vegetative growth and 28% o ... | 2008 | 17934737 |
| a new function for the bacillus pbue purine base efflux pump: efflux of purine nucleosides. | the pbue (ydhl) gene from bacillus subtilis is known to encode the purine base efflux pump, and its expression is controlled by an adenine-dependent riboswitch. we cloned the pbue gene from bacillus amyloliquefaciens and examined gene expression by its own cis-acting regulatory elements in escherichia coli. regulation of pbue expression, previously found in b. subtilis, was retained in this heterologous expression: it was induced by adenine and activated by a mutation in the 5' untranslated regi ... | 2007 | 17935948 |
| environmental dynamics of bacillus amyloliquefaciens ccmi 1051 antifungal activity under different nitrogen patterns. | the aim of this study was to evaluate the influence of environmental conditions on the antifungal activity of the bacillus sp. ccmi 1053 cultures. | 2008 | 17953685 |
| isolation of the bacillus subtilis antimicrobial peptide subtilosin from the dairy product-derived bacillus amyloliquefaciens. | to purify and characterize an antimicrobial protein (bacteriocin) isolated from the dairy product-derived bacillus amyloliquefaciens. | 2008 | 17976171 |
| cholesterol lowering mechanism of soybean protein hydrolysate. | numerous attempts have been made to find the mechanism and component of the cholesterol lowering activity of soybean. in this study, it was proved that the peptides in soybean protein hydrolysate (sph) made by certain proteases have a hypocholesterolemic effect. among the mechanisms suggested, that is, blockage of bile acid and/or cholesterol absorption, inhibition of cholesterol synthesis, and stimulation of low-density lipoprotein receptor (ldl-r) transcription, sph appeared to stimulate ldl-r ... | 2007 | 18052124 |
| effect of cultivation conditions on spore production from bacillus amyloliquefaciens b128 and its antagonism to botrytis elliptica. | to maximize spore production by bacillus amyloliquefaciens b128, and its antagonism to the fungal pathogen botrytis elliptica b061. | 2008 | 18179538 |
| isolation and characterization of antagonistic bacteria against bacterial leaf spot of euphorbia pulcherrima. | to investigate the inhibition potential of leaf-associated bacteria against the pathogen of bacterial leaf spot of euphorbia pulcherrima. | 2008 | 18298451 |
| bacillus velezensis is a later heterotypic synonym of bacillus amyloliquefaciens. | strain bcrc 14193, isolated from soil, shared more than 99 % 16s rrna gene sequence similarity with bacillus amyloliquefaciens bcrc 11601(t) and bacillus velezensis bcrc 17467(t). this strain was previously identified as b. amyloliquefaciens, based on dna-dna hybridization, but its dna relatedness value with b. velezensis bcrc 17467(t) was 89 %. to investigate the relatedness of strain bcrc 14193, b. amyloliquefaciens and b. velezensis, the partial sequence of the gene encoding the subunit b pro ... | 2008 | 18319476 |
| assessment of the role of chemotaxis and biofilm formation as requirements for colonization of roots and seeds of soybean plants by bacillus amyloliquefaciens bnm339. | this article correlates colonization with parameters, such as chemotaxis, biofilm formation, and bacterial growth, that are believed to be connected. we show here, by using two varieties of soybean plants that seeds axenically produced exudates, induced a chemotactic response in bacillus amyloliquefaciens, whereas root exudates did not, even when the exudates, also collected under axenic conditions, were concentrated up to 200-fold. root exudates did not support bacterial cell division, whereas ... | 2008 | 18335278 |
| a stochastic model for predicting dextrose equivalent and saccharide composition during hydrolysis of starch by alpha-amylase. | a stochastic model was developed that was used to describe the formation and breakdown of all saccharides involved during alpha-amylolytic starch hydrolysis in time. this model is based on the subsite maps found in literature for bacillus amyloliquefaciens alpha-amylase (baa) and bacillus licheniformis alpha-amylase (bla). carbohydrate substrates were modeled in a relatively simple two-dimensional matrix. the predicted weight fractions of carbohydrates ranging from glucose to heptasaccharides an ... | 2008 | 18351657 |
| phytate utilization by genetically engineered lysine-producing corynebacterium glutamicum. | heterologous expression of a phytase gene (phyc) from bacillus amyloliquefaciens dsm 7 enabled the growth of corynebacterium glutamicum with phytate (myo-inositol-1,2,3,4,5,6-hexakisphosphate) as a new, sole source of phosphorus. phytate was not used as a carbon source. during growth of the phyc-expressing amino acid (l-lysine)-producing strain c. glutamicum atcc 21253 (pwlq2::phyc) with phytate as the source of phosphorus, merely a small, transient accumulation of inorganic phosphate was observ ... | 2008 | 18374441 |
| genome shuffling of lactobacillus delbrueckii mutant and bacillus amyloliquefaciens through protoplasmic fusion for l-lactic acid production from starchy wastes. | current study was focused on the development of a non-fastidious lactic acid producing strain having better growth rate, low ph tolerance and good productivity by genome shuffling of a mutant strain of lactobacillus delbrueckii ncim 2025 and an amylase producing non-fastidious bacillus amyloliquefaciens atcc 23842. after the third cycle of the protoplast fusion, lactic acid production by few fusants was monitored and the best fusant was selected for further studies. optimization of the important ... | 2008 | 18482834 |
| identification of ldl-receptor transcription stimulating peptides from soybean hydrolysate in human hepatocytes. | soybean protein and its hydrolysate have been reported to have cholesterol-lowering property, but the responsible components are still largely unknown. in previous study, we found that soybean protein hydrolysate (sph) prepared with the protease from bacillus amyloliquefaciens fse-68, strongly stimulates transcription of low density lipoprotein receptor (ldl-r). to identify ldl-r transcription stimulating peptides in human hepatocytes, the sph was fractionated with gel permeation chromatograpy a ... | 2008 | 18500811 |
| the dependence of protein release from bacillus amyloliquefaciens on the growth phase in batch culture. | for the recovery of intracellular material from bacteria it is often necessary to disrupt the cells. much work has been done on the kinetics of protein release in beadmills,(1) homogenizers,(2) and by ultrasonication.(3) in this paper we report how the growth phase of bacillus amyloliquefaciens grown in batch culture affects the rate of protein release by ball milling, ultrasonication, and autolysis. we further suggest that autolysis is a feasible method for disrupting bacillus. | 1985 | 18553751 |
| barnase as a new therapeutic agent triggering apoptosis in human cancer cells. | rnases are currently studied as non-mutagenic alternatives to the harmful dna-damaging anticancer drugs commonly used in clinical practice. many mammalian rnases are not potent toxins due to the strong inhibition by ribonuclease inhibitor (ri) presented in the cytoplasm of mammalian cells. | 2008 | 18560598 |
| development of biocontrol agents from food microbial isolates for controlling post-harvest peach brown rot caused by monilinia fructicola. | an unconventional strategy of screening food microbes for biocontrol activity was used to develop biocontrol agents for controlling post-harvest peach brown rot caused by monilinia fructicola. forty-four microbial isolates were first screened for their biocontrol activity on apple fruit. compared with the pathogen-only check, seven of the 44 isolates reduced brown rot incidence by >50%, including four bacteria: bacillus sp. c06, lactobacillus sp. c03-b and bacillus sp. t03-c, lactobacillus sp. p ... | 2008 | 18573559 |
| kinetics of alpha-amylase synthesis from bacillus amyloliquefaciens. | the microbial production of alpha-amylase from bacillus amyloliquefaciens was investigated. the microorganism was grown using media containing glucose or maltose at 37 degrees c and under aerobic conditions in a 16-l fermentor. the alpha-amylase synthesis from maltose was not found to be inducible but was found to be subject to catabolite repression. the maltose uptake rate was observed to be the rate-limiting step compared to the conversion rate of maltose to glucose by intracellular alpha-gluc ... | 1988 | 18584616 |
| fed-batch fermentation for the production of alpha-amylase by bacillus amyloliquefaciens. | fed-batch cultures were performed to maximize the alpha-amylase activity in a bioreactor. kinetic equations containing a catabolite repression effect were used to model the enzyme formation from bacillus amyloliquefaciens. fed-batch culture experiments were performed using maltose to implement the optimal feeding strategy. optimal fed-batch culture based on sequential parameter estimation was performed successfully using off-line analysis while the fermentation was in progress. the enzyme activi ... | 1988 | 18584627 |
| stabilization of restriction endonuclease bam hi by cross-linking reagents. | bacillus amyloliquefaciens h produces a restriction endonuclease enzyme bamhl which is heat labile even at low temperatures. studies were conducted to enhance thermal stability of bamhl using cross-linking reagents, namely, glutaraldehyde, dimethyl adipimidate (dma), dimethyl suberimidate (dms), and dimethyl 3,3'-dithiobispropionimidate (dtbp). reaction with glutaraldehyde did not result in a preparation with enhanced thermal stability. however, the dma-, dms-, and dtbp-cross-linked preparations ... | 1989 | 18587865 |
| cell growth and alpha-amylase production characteristics of bacillus amyloliquefaciens. | growth and alpha-amylase production characteristics of bacillus amyloliquefaciens strain f (atcc 23350) in batch cultures are examined using glucose or maltose as the carbon source. while the cell growth is rapid when glucose is used as the carbon source, higher cell mass, higher total and specific enzyme activities, and higher enzyme production rates are obtained when maltose is used as the carbon source. the overall specific enzyme activity decreases with an increase in the initial concentrati ... | 1989 | 18587901 |
| effect of yeast extract on alpha-amylase synthesis by bacillus amyloliquefaciens. | | 1989 | 18587980 |
| cell growth and alpha-amylase production characteristics of bacillus amyloliquefaciens. | | 1989 | 18588140 |
| inactivation and stabilization of stabilisins in neat organic solvents. | the stability of the serine proteases from bacillus amyloliquefaciens (subtillisin bpn') and bacillus licheniformis (subtilisin carlsberg) was investigated in various anhydrous solvents at 45 degrees c. the half-life of subtilisin bpn' in dimethyl-formamide dramatically depends on the ph of the aqueous solutions from which the enzyme was lyophilized, increasing from 48 min to 20 h when the ph is raised from 6.0 to 7.9. both subtilisins exhibited substantial inactivation during multihour incubati ... | 1991 | 18600863 |
| simple structured model for alpha-amylase synthesis by bacillus amyloliquefaciens. | a predictive, simple, structured model describing the synthesis of alpha-amylase by bacillus amyloliquefaciens was formulated. three key intracellular processes were identified (i.e, translation, and excretion) along with two key intracellular components (i.e., mrna and the intracellular form of the alpha-amylase enzyme). nearly all the model parameters were estimated by means of performing independent experiments, primarily fed-batch experiments. the model was shown to predict transient system ... | 1991 | 18600872 |
| auxin production and detection of the gene coding for the auxin efflux carrier (aec) protein in paenibacillus polymyxa. | different species of paenibacillus are considered to be plant growth-promoting rhizobacteria (pgpr) due to their ability to repress soil borne pathogens, fix atmospheric nitrogen, induce plant resistance to diseases and/or produce plant growth-regulating substances such as auxins. although it is known that indole-3-acetic acid (iaa) is the primary naturally occurring auxin excreted by paenibacillus species, its transport mechanisms (auxin efflux carriers) have not yet been characterized. in this ... | 2008 | 18604494 |
| transition state regulator abrb inhibits transcription of bacillus amyloliquefaciens fzb45 phytase through binding at two distinct sites located within the extended phyc promoter region. | we have previously identified the phyc gene of bacillus amyloliquefaciens fzb45, encoding extracellular phytase, as a member of the phop regulon, which is expressed only during phosphate starvation. its sigma(a)-dependent promoter is positively and negatively regulated by the phosphorylated phop response regulator in a phosphate-dependent manner (o. makarewicz, s. dubrac, t. msadek, and r. borriss, j. bacteriol. 188:6953-6965, 2006). here, we provide experimental evidence that the transcription ... | 2008 | 18676675 |
| biochemical characterization of raw-starch-digesting alpha amylase purified from bacillus amyloliquefaciens. | alpha amylase (e.c. 3.2.1.1) of bacillus amyloliquefaciens produced by submerged fermentation was purified to near homogeneity by ion exchange chromatography. through the process 38.6-fold increase in purity with a specific activity of 72 u/mg proteins was obtained. the apparent molecular weight of the purified enzyme was found to be 58 kda by sds-page. the enzyme was relatively stable between ph 5.0-8.0 and temperature between 50 and 60 degrees c. the enzyme did not show any obligate requiremen ... | 2009 | 18769877 |
| phage mu-driven two-plasmid system for integration of recombinant dna in the methylophilus methylotrophus genome. | a phage mu-driven two-plasmid system for dna integration in escherichia coli genome has been adjusted for methylophilus methylotrophus. constructed helper plasmids with broad-host-range replicons carry thermo-inducible genes for transposition factors mua and mub. integrative plasmids that are only replicated in e. coli could be mobilized to m. methylotrophus and contained mini-mu unit with a short terminus of mu dna, mu-attl/r. mini-mu unit was integrated in the m. methylotrophus genome via mobi ... | 2008 | 18820908 |
| interactions of bacillus spp. and plants--with special reference to induced systemic resistance (isr). | biological control of soil-borne pathogens comprises the decrease of inoculum or of the disease producing activity of a pathogen through one or more mechanisms. interest in biological control of soil-borne plant pathogens has increased considerably in the last few decades, because it may provide control of diseases that cannot or only partly be managed by other control strategies. recent advances in microbial and molecular techniques have significantly contributed to new insights in underlying m ... | 2009 | 18845426 |
| antimicrobial activity of culture filtrate of bacillus amyloliquefaciens rc-2 isolated from mulberry leaves. | abstract a potential antagonist, bacillus amyloliquefaciens strain rc-2, against colletotrichum dematium, mulberry anthracnose fungus, was obtained from healthy mulberry leaves by in vitro and in vivo screening techniques. application of culture filtrate of rc-2 inhibited disease on mulberry leaves, indicating that suppression was due to antifungal compounds in the filtrate. development of mulberry anthracnose on mulberry leaves was inhibited only when the culture filtrate was applied before fun ... | 2001 | 18944392 |
| more than anticipated - production of antibiotics and other secondary metabolites by bacillus amyloliquefaciens fzb42. | the genome of environmental bacillus amyloliquefaciens fzb42 harbors numerous gene clusters involved in synthesis of antifungal and antibacterial acting secondary metabolites. five gene clusters, srf, bmy, fen, nrs, dhb, covering altogether 137 kb, direct non-ribosomal synthesis of the cyclic lipopeptides surfactin, bacillomycin, fengycin, an unknown peptide, and the iron siderophore bacillibactin. bacillomycin and fengycin were shown to act against phytopathogenic fungi in a synergistic manner. ... | 2009 | 18957859 |
| a novel vector for direct cloning pcr fragments by positive selection based on the lethal barnase. | a novel vector for direct pcr fragments cloning by positive selection, pbn, was constructed based on the lethal barnase from bacillus amyloliquefaciens. barnase was modified by inserting an additional insert at a pivotal ile-54 site, which could take crucial affect on protein structure and absolute activity. the lacz' expressing cassette of puc19 was replaced by the modified barnase under the nptiii promoter. this novel vector could exist in large quantities as puc19 in e. coli hosts. for the di ... | 2009 | 18979229 |
| genome analysis of bacillus amyloliquefaciens fzb42 reveals its potential for biocontrol of plant pathogens. | the genome of plant-associated bacillus amyloliquefaciens fzb42 harbors an array of giant gene clusters involved in synthesis of lipopeptides and polyketides with antifungal, antibacterial and nematocidal activity. five gene clusters, srf, bmy, fen, nrs, dhb, covering altogether 137 kb, were shown to direct synthesis of the cyclic lipopeptides surfactin, bacillomycin, fengycin, an unknown peptide, and the iron-siderophore bacillibactin. in addition, one gene cluster encoding enzymes involved in ... | 2009 | 19041913 |
| difficidin and bacilysin produced by plant-associated bacillus amyloliquefaciens are efficient in controlling fire blight disease. | representatives of bacillus amyloliquefaciens were shown to possess biocontrol activity against fire blight, a serious disease of orchard trees caused by erwinia amylovora. genome analysis of b. amyloliquefaciens fzb42 identified gene clusters responsible for synthesis of several polyketide compounds with antibacterial action. we show here that the antibacterial polyketides difficidin and to a minor extent bacillaene act efficiently against e. amylovora. surprisingly, a mutant strain blocked in ... | 2009 | 19061923 |
| peptide synthesis using carbamoylmethyl esters as acyl donors mediated by bacillus amyloliquefaciens protease. | bacillus amyloliquefaciens protease-catalyzed peptide bond formation was investigated using the carbamoylmethyl (cam) ester as the acyl donor. a series of n-protected l-amino acid cam esters were coupled with an l-amino acid amide in acetonitrile in good to excellent yields. the superiority of the cam ester for segment condensations was demonstrated in several 2+1 couplings. furthermore, the couplings of racemic amino acid cam esters as carboxyl components afforded the l-l-peptides in a highly d ... | 2008 | 19075814 |
| denaturation of bacillus amyloliquefaciens alpha-amylase with urea. | the urea induced denaturation of the bacillus amyoliqefaciens alpha-amylase (e.c. 3.2.1.1) was studied by absorption measurements in the near ultra-violet region and specific activity measurements. spectral measurements were made at ph 6.9 and over the temperature range 20-80 degrees c. it has been observed that urea induced a cooperative transition. in the absence of denaturant, the gibs energy changes were in the range of 8-15 kcal mol(-1). alpha-amylase lost 80% of its activity in the concent ... | 2007 | 19090116 |
| physiological responses of bacillus amyloliquefaciens spores to high pressure. | pressure inactivation behavior of bacillus amyloliquefaciens spores was investigated in deionized water. the spores of b. amyloliquefaciens were subjected to 105 degrees c and 700 mpa. the magnitude of the decrease in viability after pressure treatment was similar to that after pressure treatment followed by heat shock. the increase of dipicolinic acid (dpa) release was correlated with the spore inactivation, and the hydrophobicity did not significantly change during the pressure-assisted therma ... | 2007 | 18050959 |
| an antifungal protein from bacillus amyloliquefaciens. | to isolate and characterize an antifungal protein from the culture broth of the bacterium bacillus amyloliquefaciens. | 2008 | 19120637 |
| transcript profiling of oilseed rape (brassica napus) primed for biocontrol differentiate genes involved in microbial interactions with beneficial bacillus amyloliquefaciens from pathogenic botrytis cinerea. | many microorganisms interact with plants but information is insufficient concerning requirements for plant colonization and if interactions become beneficial or detrimental. pretreatment of oilseed rape (brassica napus) with bacillus results in disease suppression upon challenge with pathogens. we have studied transcriptome effects on oilseed rape primed with the bacillus amyloliquefaciens 5113 biocontrol strain and compared that with effects of the fungal pathogen botrytis cinerea. using the cd ... | 2009 | 19184461 |
| intein-mediated protein assembly in transgenic wheat: production of active barnase and acetolactate synthase from split genes. | engineering traits by the assembly of non-functional gene products is a promising tool for modern plant biotechnology. in this article, we describe the establishment of male sterility and herbicide resistance in wheat (triticum aestivum) by complementing inactive precursor protein fragments through a split intein system. n- and c-terminal fragments of a barnase gene from bacillus amyloliquifaciens were fused to intein sequences from the synechocystis sp. gene dnab and delivered into the wheat ge ... | 2009 | 19222807 |
| biological interactions to select biocontrol agents against toxigenic strains of aspergillus flavus and fusarium verticillioides from maize. | biological control represent an alternative to the use of pesticides in crop protection. a key to progress in biological control to protect maize against fusarium verticillioides and aspergillus flavus maize pathogens are, to select in vitro, the best agent to be applied in the field. the aim of this study was to examine the antagonistic activity of bacterial and yeast isolates against f.verticillioides and a. flavus toxigenic strains. the first study showed the impact of bacillus amyloliquefaci ... | 2009 | 19247799 |