| efficacy of ethylenediamine dihydriodide as an agent to prevent experimentally induced bovine foot rot. | ethylenediamine dihydriodide (eddi) was administered by capsule and as a feed premix to cattle in 2 studies. in study i (32 steers), eddi was given at 0, 12.5, 50, and 200 mg/animal each day and in study ii (36 steers) at 0, 30, and 200 mg/animal each day. serum iodine levels were monitored during the studies. the cattle were inoculated intradermally in the interdigital space with a mixture of fusobacterium necrophorum and bacteroides melaninogenicus to induce acute foot rot at day 15 (study i) ... | 1984 | 6146277 |
| in vitro activity of ticarcillin plus clavulanic acid against bacteria isolated in three centers. | over 10,000 bacterial isolates were tested against ticarcillin alone or combined with clavulanic acid (2.0 micrograms/ml). of 5,295 enterobacteriaceae, 72% were susceptible to ticarcillin alone, whereas 91% were susceptible to the combination. bacteroides fragilis and bacteroides melaninogenicus isolates were more susceptible to the combination. the activity of ticarcillin against other microorganisms was not profoundly influenced. | 1984 | 6147246 |
| postsurgical life-threatening bacteroides infection. | | 1984 | 6147280 |
| purification and characterization of an immunoglobulin a1 protease from bacteroides melaninogenicus. | attention has recently been focused on bacterial proteases with the capacity to cleave immunoglobulin a (iga proteases) as possible pathogenic factors in bacterial meningitis, gonorrhoea, and destructive periodontal disease. here, we describe a method for the rapid purification of a specific iga1 protease from bacteroides melaninogenicus. the iga1 protease was purified 6,172-fold with a yield of 9% by ammonium sulfate precipitation, deae-ion exchange chromatography, and separation on a preparati ... | 1984 | 6147309 |
| [the role of bacteroides melaninogenicus in periodontitis]. | | 1983 | 6150566 |
| [anaerobic sepsis in infants]. | | 1984 | 6150670 |
| anaerobic bacteroides melaninogenicus--oral sexually-urethritis: a case report. | | 1984 | 6150963 |
| citrobacter diversus meningitis and brain abscess associated with bacteroides melaninogenicus. | | 1984 | 6151172 |
| efficacy of ethylenediamine dihydriodide in the prevention of naturally occurring foot rot in cattle. | the efficacy of an ethylenediamine dihydriodide containing salt mixture was evaluated with regard to the prevention of naturally occurring foot rot in pastured cattle. ninety-six cattle were assigned to the treatment group and 96 were assigned to the control group. the groups were allowed consumption of salt-mineral mixtures, ad libitum, that were identical, except with respect to iodine concentration. the control group's mixture contained 0.0025% iodine and the treatment group's mixture contain ... | 1984 | 6151816 |
| [immuno-serological studies in experimental gingivitis in macaca irus]. | | 1984 | 6151859 |
| [fibrinolytic substance of bacteroides melaninogenicus isolated from periodontal pocket and its activity accelerated by saliva]. | | 1984 | 6151860 |
| [detection of bacteroides melaninogenicus and bacteroides fragilis from infected root canals of primary teeth and children's mouths using a fluorescent antibody test kit]. | | 1984 | 6152567 |
| [method of detecting b. melaninogenicus]. | | 1981 | 6164842 |
| an electron microscopic study of surface polysaccharides in bacteroides. | the electron microscopic appearance of the cell surface of bacteroides strains and klebsiella pneumoniae stained with ruthenium red or colloidal iron is described. the effect of polymyxin b (pmb) was also registered. it was found that all bacteroides strains have a polysaccharide lined 'micro-capsule' external to the outer membrane which could aggregate and form blebs. the blebs so formed were distinct from other types of bleb formed in klebsiella involving the outer membrane and induced by pmb. ... | 1982 | 6186892 |
| evaluation of periodontal treatments using controlled-release tetracycline fibers: microbiological response. | in a 12-month multi-center study of 116 adult periodontitis subjects, six putative periodontal pathogens were monitored by dna probe methods in a subset of 31 subjects. monitored species included porphyromonas gingivalis (pg), prevotella intermedia (pi), fusobacterium nucleatum (fn), eikenella corrodens (ec), campylobacter rectus (cr), and actinobacillus actinomycetemcomitans (aa) with an average detection limit of 1.8 x 10(4) bacterial colony forming units/sample. the microbiological response t ... | 1995 | 7473013 |
| identifying and isolating fimbrial-associated adhesins of oral gram-negative bacteria. | | 1995 | 7476403 |
| effective method for discriminating between oral bacterial and human alkaline phosphatase activity. | alkaline phosphatase (alpase) activity was quantitatively compared in various kinds of oral bacteria. high alpase activity was detected in 3 species of periodontal bacteria, porphyromonas gingivalis, prevotella intermedia and capnocytophaga sputigena. the alpase activity detected in these bacteria was almost completely inhibited in the presence of 1% sodium dodecyl sulfate (sds). by contrast, the activity of mammalian alpase isoenzymes was not inhibited at all even in the presence of 1% sds. the ... | 1994 | 7478753 |
| use of the syrian golden hamster for the induction of intraoral abscesses by sutures contaminated with human subgingival plaque. | analysis of normal oral flora in 150 cheek pouches of hamsters (mesocricetus auratus) defined the microbial working environment and demonstrated the absence of human oral black-pigmented bacteria. silk sutures saturated with porphyromonas gingivalis, prevotella intermedia or subgingival plaque were used to close wounds made in hamster's cheek pouches. abscesses were formed when sutures had solitary p. gingivalis or other bacteria mixed with p. gingivalis or when p. intermedia was mixed with othe ... | 1994 | 7478755 |
| the antimicrobial activity of essential oils and essential oil components towards oral bacteria. | a method for reproducibly determining minimal inhibitory concentrations and minimal bactericidal concentrations of plant extracts towards fastidiously and facultatively anaerobic oral bacteria, predicated upon measurements of optical densities in microtitre plate wells, was devised. the antimicrobial properties of some botanical oils were surveyed; of these, australian tea tree oil, peppermint oil, and sage oil proved to be the most potent essential oils, whereas thymol and eugenol were potent e ... | 1994 | 7478759 |
| effect of proteolytic enzymes on the lysis and growth of oral bacteria. | previous studies have shown increased levels of proteolytic enzymes in affected periodontal sites. the aim of the present investigation was to evaluate the effect of proteolytic environments on the lysis and growth of selected oral bacteria associated with either healthy or diseased periodontal sites. the effect of trypsin, chymotrypsin and proteinase k on cell lysis was determined following incubation with bacteria, whereas the effect of the same proteolytic enzymes on bacterial growth was test ... | 1994 | 7478762 |
| the tet(q) gene in bacteria isolated from patients with refractory periodontal disease. | twenty-two tetracycline-resistant (tetr) anaerobic and facultative anaerobic bacteria isolated from periodontal pockets of 12 patients with refractory periodontitis were examined for the presence of the tet q determinant by dna-dna hybridization. dot blots of bacterial dna were tested with an intragenic digoxigenin-labelled tet(q) probe consisting of a 1.45 kb ecori/pvuii fragment from plasmid pnfd13-2. southern blots of chromosomal dna digested with the restriction enzyme ecori were also examin ... | 1994 | 7478767 |
| a xylan hydrolase gene cluster in prevotella ruminicola b(1)4: sequence relationships, synergistic interactions, and oxygen sensitivity of a novel enzyme with exoxylanase and beta-(1,4)-xylosidase activities. | two genes concerned with xylan degradation were found to be closely linked in the ruminal anaerobe prevotella ruminicola b(1)4, being separated by an intergenic region of 75 nucleotides. xyna is shown to encode a family f endoxylanase of 369 amino acids, including a putative amino-terminal signal peptide. xynb encodes an enzyme of 319 amino acids, with no obvious signal peptide, that shows 68% amino acid identity with the xsa product of bacteroides ovatus and 31% amino acid identity with a beta- ... | 1995 | 7487028 |
| comparison of host tissue and bacterial dipeptidyl peptidases in human gingival crevicular fluid by analytical isoelectric focusing. | earlier work has shown that gingival crevicular fluid (gcf) contains dipeptidyl peptidase (dpp) activities that resemble those in host tissue. here, further comparisons were made with enzymes from suspected periodontal pathogens. gingival tissue and gcf were collected from patients with chronic periodontitis. dpp ii and dpp iv fractions with acid and alkaline ph optima, respectively, were separated from crude tissue extracts by gel-filtration chromatography. bacterial cell sonicates were prepare ... | 1995 | 7487574 |
| clinical and microbiological features of necrotizing fasciitis. | the microbiological and clinical characteristics of 83 patients with necrotizing fasciitis (nf) treated over a period of 17 years are presented. bacterial growth was noted in 81 of 83 (98%) of specimens from patients with nf. aerobic or facultative bacteria only were recovered in 8 (10%) specimens, anaerobic bacteria only were recovered in 18 (22%) specimens, and mixed-aerobic-anaerobic floras were recovered in 55 (68%) specimens. in total, there were 375 isolates, 105 aerobic or facultative bac ... | 1995 | 7494032 |
| influence of periodontal bacteria and disease status on v beta expression in t cells. | some bacterial antigens such as s. aureus enterotoxins can selectively stimulate t cells that express specific v beta genes of the t cell antigen receptor (tcr). the purpose of this study was to investigate whether or not periodontal bacteria could similarly alter the expression of v beta families within the tcr complex. peripheral blood mononuclear cells (pbmncs) were isolated from 12 patients with early onset periodontitis and 11 periodontally-healthy controls. pbmncs were incubated in media a ... | 1995 | 7494180 |
| demonstration of human immunoglobulin g fc-binding activity in oral bacteria. | nonimmune binding of immunoglobulins via the fc fragment may reduce opsonization and phagocytosis of bacteria and is thus considered a virulence factor. the aim of this study was to investigate a wide range of oral bacterial strains for the presence of human immunoglobulin g (igg) fc-binding activity. a total of 132 strains representing 40 different gram-positive and gram negative bacterial species were tested for igg fc-binding activity by using a fast and simple dot blot procedure with horsera ... | 1994 | 7496956 |
| effect of candida albicans infection and clotrimazole treatment on vaginal microflora in vitro. | to determine the effect of candida albicans infection and clotrimazole treatment on vaginal microflora. | 1995 | 7501341 |
| simultaneous hybridization and subsequent colour detection of subgingival bacterial dna on colony lifts. | this paper reports the development of a protocol allowing hybridization and detection of dna fixed to nylon colony lifts from up to three species of bacteria simultaneously. half ml samples of serial dilutions of pure cooked-meat broth (cmb) cultures of capnocytophaga ochracea, actinobacillus actinomycetemcomitans and prevotella intermedia were grown on trypticase soy blood agar (tsba) plates for 7 days in an anaerobic chamber. from the same cmbs a further set of dilutions was completed that con ... | 1993 | 7507660 |
| the lipopolysaccharide of porphyromonas gingivalis is not antigenically cross-reactive with that of other species. | large numbers of porphyromonas, prevotella, and bacteroides strains were screened by 3 monoclonal antibodies (mabs) and 8 rabbit antisera raised against porphyromonas gingivalis, in order to detect any possible recognition of non-p. gingivalis surface antigens by these immunoreagents. all three mabs, which were lps-specific, extensively recognized lps from 10 p. gingivalis strains in immunoblotting, whereas they recognized none of the 34 non-p. gingivalis strains. rabbit antisera were similarly ... | 1994 | 7513001 |
| anaerobes in polymicrobial surgical infections: incidence, pathogenicity, and antimicrobial resistance. | many types of anaerobic bacteria have been isolated from clinical infections. although most of these infections are polymicrobial and involve facultative gram-negative bacilli, some are strictly anaerobic. for most of them, surgical intervention such as drainage of an abscess and debridement of devitalised tissue is the primary treatment and re-establishes good blood flow to the affected area. appropriate antimicrobial treatment is also important to kill both residual organisms and those that ma ... | 1994 | 7524793 |
| effects of cytokines and periodontopathic bacteria on the leukocyte function-associated antigen 1/intercellular adhesion molecule 1 pathway in gingival fibroblasts in adult periodontitis. | we investigated the effects of inflammatory cytokines and periodontopathic bacteria on expression of intercellular adhesion molecule 1 (icam-1), vascular cell adhesion molecule 1, and e-selectin (endothelial leukocyte adhesion molecule 1) in cultured human gingival fibroblasts (hgf). cell surface icam-1 was upregulated on hgf under transcriptional control by exposure not only to interleukin-1 beta, tumor necrosis factor alpha, and gamma interferon but also to sonic extracts prepared from porphyr ... | 1994 | 7525481 |
| comparison of utilization of pectins from various sources by pure cultures of pectinolytic rumen bacteria and mixed cultures of rumen microorganisms. | utilization of citrus, lucerne, apple and sugar beet pulp pectins by pure strains of rumen bacteria, prevotella ruminicola, lachnospira multiparus and butyrivibrio fibrisolvens was compared. additionally, the utilization of pectins by mixed rumen microorganisms was evaluated. the comparison was based on the depletion of galacturonic acid from medium, content of cellular protein in the cultures and the amount of end products of pectin fermentation in cell-free culture fluids. it was found that ci ... | 1994 | 7526615 |
| cloning, characterization, and antigen specificity of t-lymphocyte subsets extracted from gingival tissue of chronic adult periodontitis patients. | chronic periodontitis is characterized by dense infiltrations of b and t lymphocytes within the gingival connective tissue. distinct anaerobic gram-negative bacteria as well as autoimmunity to collagen have been reported to play a role in the etiology and the pathogenesis of this disease. here we describe the cloning and characterization of cd4+ and cd8+ t lymphocytes isolated from inflamed gingival tissue obtained from four patients with chronic periodontitis. clones were raised with phytohemag ... | 1995 | 7539406 |
| lipopolysaccharides from periodontal pathogens prime neutrophils for enhanced respiratory burst: differential effect of a synthetic lipid a precursor iva (la-14-pp). | when neutrophils are incubated with bacterial lipopolysaccharide (lps), they become primed for enhanced release of superoxide anion (o2-) in response to stimulation by fmlp. we investigated the human neutrophil-priming activity of lps from the periodontal pathogens, porphyromonas gingivalis (pg), prevotella intermedia (pi) and actinobacillus actinomycetemcomitans (aa) in comparison with that of lps from escherichia coli (e. coli). the optimum conditions for lps to prime neutrophils were assessed ... | 1995 | 7539837 |
| 16s rrna gene similarities indicate that hallella seregens (moore and moore) and mitsuokella dentalis (haapsalo et al.) are genealogically highly related and are members of the genus prevotella: emended description of the genus prevotella (shah and collins) and description of prevotella dentalis comb. nov. | because of similarities in the cellular fatty acid compositions of hallella seregens and mitsuokella dentalis, we determined the 16s rrna gene sequences of the type strains of these species to assess their relationship. a very high level of sequence relatedness (approximately 99.8%) was found between h. seregens and m. dentalis, indicating that these species are genealogically closely related. a comparative sequence analysis revealed that these two species are members of the genus prevotella and ... | 1995 | 7547307 |
| comparative studies of the iga1 protease genes from haemophilus influenzae, neisseria gonorrhoeae, and prevotella melaninogenica. | | 1995 | 7548543 |
| role of the reference laboratory in susceptibility testing of anaerobes and a survey of isolates referred from laboratories in england and wales during 1993-1994. | methods for susceptibility testing of anaerobes and their patterns of susceptibility remain debatable. the responsibilities of the public health laboratory service anaerobe reference unit include identification of referred isolates; development of methods; monitoring of the occurrence of anaerobic infections; performance of taxonomic studies; maintenance of a reference collection; and monitoring of the antibiotic susceptibility of clinical isolates. selection of antibiotics for anaerobic infecti ... | 1995 | 7548547 |
| update on the taxonomy and the clinical and laboratory characteristics of pigmented anaerobic gram-negative rods. | pigmented anaerobic gram-negative rods are currently categorized as 17 species distributed in three genera: prevotella, porphyromonas, and bacteroides. these organisms are often encountered in clinical specimens but are also found as part of the indigenous flora on various mucosal surfaces. several studies are presently assessing the association of individual species with health and disease. for example, porphyromonas gingivalis and porphyromonas endodontalis are key putative pathogens in adult ... | 1995 | 7548548 |
| enzymatic profiles of prevotella, porphyromonas, and bacteroides species obtained with the api zym system and rosco diagnostic tablets. | | 1995 | 7548549 |
| comparison of the use of oligonucleotide probes, 4-methylumbelliferyl derivatives, and conventional methods for identifying prevotella bivia. | | 1995 | 7548550 |
| bacteriology of chronic otitis media, chronic sinusitis, and paranasal mucopyocele in japan. | a bacteriologic study of cases of chronic otitis media, chronic sinusitis, and paranasal mucopyocele diagnosed at gifu university hospital was performed between 1989 and 1992. bacteria were isolated from all of 13 samples of discharge from the ears of patients with chronic otitis media, all of 14 mucosal biopsy specimens from patients with chronic sinusitis, 8 of 10 aspirate samples from patients with chronic sinusitis, and 9 of 15 aspirates samples from patients with mucopyocele. cultures often ... | 1995 | 7548557 |
| immune response to fusobacterium nucleatum and prevotella intermedia in patients with peritonsillar cellulitis and abscess. | | 1995 | 7548558 |
| a retrospective review of cases of anaerobic empyema and update of bacteriology. | we conducted a retrospective study to update the bacteriology of 46 cases of anaerobic empyema that were originally studied between 1976 and 1993 at the wadsworth anaerobic bacteriology clinical research laboratory (los angeles). anaerobic bacteriologic studies were completed for all 46 pleural fluid specimens, and aerobic bacteriologic studies were completed for 41 of these specimens. thirty-seven clinical charts were available for review. a total of 161 anaerobic isolates (3.5 per patient) rep ... | 1995 | 7548560 |
| role of bacterial vaginosis in pelvic inflammatory disease. | pelvic inflammatory disease (pid) is a frequent infection in sexually active young women and results in adverse sequelae, including tubal-factor infertility and ectopic pregnancy. in the 1970s investigations using culdocentesis demonstrated that anaerobic bacteria played an important role in the etiology of pid. this finding has subsequently been confirmed by studies utilizing laparoscopy and/or endometrial biopsy to obtain specimens directly from the upper genital tract (uterine cavity and fall ... | 1995 | 7548573 |
| bacteriology of skin and soft-tissue infections: comparison of infections in intravenous drug users and individuals with no history of intravenous drug use. | the bacteriology of cutaneous or subcutaneous abscesses (86 specimens) among intravenous drug users (ivdus) was compared with the bacteriology of abscesses (74 specimens) in patients with no history of intravenous drug use (non-ivdus). the ivdu abscesses yielded 173 aerobes and 131 anaerobes. staphylococcus aureus was the most common aerobe isolated (50% of specimens yielded this isolate), followed by "streptococcus milleri" (46%). the commonly encountered anaerobes were fusobacterium nucleatum ... | 1995 | 7548575 |
| detection of putative periodontal pathogens in subgingival specimens by 16s ribosomal dna amplification with the polymerase chain reaction. | the utility of the 16s ribosomal rna-based polymerase chain reaction (pcr) for detection of actinobacillus actinomycetemcomitans, bacteroides forsythus, campylobacter rectus, eikenella corrodens, porphyromonas gingivalis, prevotella intermedia, and treponema denticola was examined and compared with that of anaerobic culture. primer pairs consisting of 20-27 nucleotides amplified 404- to 688-bp regions of 16s ribosomal rna genes of these organisms. this method had a lower detection limit of 50 ta ... | 1995 | 7548580 |
| microbiological study of juvenile periodontitis in chile. | | 1995 | 7548581 |
| occurrence of gram-negative black-pigmented anaerobes in subgingival plaque during the development of canine periodontal disease. | | 1995 | 7548584 |
| beta-lactamase production and penicillin susceptibility among different ribotypes of prevotella melaninogenica simultaneously colonizing the oral cavity. | | 1995 | 7548599 |
| oligonucleotide probes to the 16s ribosomal rna: implications of sequence homology and secondary structure with particular reference to the oral species prevotella intermedia and prevotella nigrescens. | eight oligonucleotides based upon regions of the small subunit 16s ribosomal rna gene sequences were analysed against a background of their position within the molecule and their two-dimensional structure to rationalise their use in recognising prevotella intermedia and prevotella nigrescens. the 41 clinical isolates from both oral and respiratory sites and two reference strains were subjected to dna-dna hybridisation and multilocus enzyme electrophoresis to confirm their identity. alignment of ... | 1995 | 7553378 |
| identification of a chromosomally encoded kanamycin acetylase in porphyromonas gingivalis. | sonic extracts from the test strains of porphyromonas gingivalis, prevotella intermedia and prevotella nigrescens were tested for the ability to inactivate kanamycin sulfate in the presence of atp or acetyl coenzyme a (acetyl-coa). residual kanamycin activity was determined by a bio-assay using escherichia coli jm109 as the assay organism. sonic extracts of all test strains of p. gingivalis inactivated kanamycin. all strains tested in this study required the presence of acetyl-coa for inactivati ... | 1995 | 7557341 |
| prevalence of penicillin resistant bacteria in acute suppurative oral infection. | pus aspirated from acute suppurative oral infections in 78 patients (age range 13-76 years) yielded a total of 331 bacterial strains consisting of 143 facultative anaerobes (predominantly streptococcus spp.) and 188 strict anaerobes (predominantly prevotella spp.). seventy-five isolates (23%) were resistant to penicillin (mic > 1 mg/l), 37 (11%) were resistant to ampicillin (mic > 2 mg/l) and 16 (5%) isolates were resistant to amoxycillin/clavulanic acid (mic > 2 mg/l). samples from 43 (55%) of ... | 1995 | 7559190 |
| microbiology of secondary bacterial infection in scabies lesions. | aerobic and anaerobic bacteria were grown from specimens obtained from 30 children with secondarily infected scabies lesions. aerobic or facultative bacteria only were present in 14 (47%) patients, anaerobic bacteria only were present in 6 (20%) patients, and a mixed anaerobic-aerobic flora was present in 10 (33%) patients. fifty isolates were recovered (1.7 per specimen); 27 were aerobic or facultative bacteria and 23 were strict anaerobes. the predominant aerobic and facultative bacteria were ... | 1995 | 7559963 |
| some suspected periodontopathogens and serum antibody response in adult long-duration insulin-dependent diabetics. | the subgingival microflora and serum antibody response were examined in long-duration insulin-dependent diabetics and age- and sex-matched non-diabetics. the material consisted of 9 diabetics aged 40-49 years and 19 aged 50-59 years, 13 non-diabetics aged 40-49 years and 21 aged 50-59 years. the bacterial species studied (actinobacillus actinomycetemcomitans, campylobacter rectus, capnocytophaga spp, eikenella corrodens, fusobacterium nucleatum, porphyromonas gingivalis, prevotella intermedia) w ... | 1995 | 7560223 |
| effect of periodontal treatments on serum igg antibody titers against periodontopathic bacteria. | serum igg antibody titers to 7 periodontopathic bacteria in periodontitis patients were measured at the 1st visit and after various periodontal treatments with clinically successful improvement, in order to evaluate what kind of factors are associated with changes of serum antibody titers. 20 patients (10 male and 10 female from 23 to 61 years old) with adult, rapidly progressive periodontitis were enrolled in this study. all patients received initial preparation and most of them also underwent ... | 1995 | 7560233 |
| a 6-month home-usage trial of 0.1% and 0.2% delmopinol mouthwashes (ii). effects on the plaque microflora. | the effects of 0.1% and 0.2% delmopinol mouthwashes on supragingival plaque flora were investigated in a 6-month home-use study. 141 subjects were studied from whom plaque was collected at baseline, 12, 24, and 36 weeks. overall, there were no consistent effects on microscopic or total counts. however, there was a significant reduction in the proportion of dextran-producing streptococci in the active groups compared to the control group throughout treatment. there was no colonisation by candida ... | 1995 | 7560235 |
| volatile fatty acids, metabolic by-products of periodontopathic bacteria, inhibit lymphocyte proliferation and cytokine production. | short-chain fatty acids are a major by-product of anaerobic metabolism and can be detected in gingival fluid from periodontal pockets. since most t cells are present subjacent to the pocket epithelium in conjunction with the plasma cells, it is important to know how these t cells are affected by short-chain fatty acids produced by subgingival plaque. the purpose of this study is to examine the effects of extracellular metabolites from periodontopathic bacteria on the proliferation and cytokine p ... | 1995 | 7560387 |
| bacterial colonization on titanium, hydroxyapatite, and amalgam surfaces in vivo. | a study was conducted to evaluate qualitative and quantitative differences in bacterial colonization on titanium, hydroxyapatite, and amalgam surfaces in vivo. six healthy adult individuals participated in the study. two pieces each of titanium, hydroxyapatite, and amalgam of similar size were placed in cobalt-chromium splints and kept intra-orally in each individual for 10 min, and 1, 3, 6, 24, and 72 hrs. after removal of the splints, the pieces were rinsed in pbs and transferred to transport ... | 1995 | 7560424 |
| in vitro activity of bay y 3118, and nine other antimicrobial agents against anaerobic bacteria. | the antibacterial activity of bay y 3118, a new chlorofluoroquinolone, was determined against 257 strains of anaerobic bacteria and compared with the activities of ofloxacin, ciprofloxacin, sparfloxacin, imipenem, cefoxitin, clindamycin, chloramphenicol, metronidazole, and ornidazole. overall, bay y 3118 was the most active agent tested against the bacteroides fragilis group. its activity (mic90, 0.5 mg/l) was 16-fold lower than that of sparfloxacin (mic90, 8 mg/l), and more than 100-fold lower ... | 1995 | 7562012 |
| characterisation of black-pigmented anaerobes isolated from diseased and healthy periodontal sites. | prevotella intermedia has recently been re-defined and a new species, prevotella nigrescens has been proposed. however, there is little data available on the incidence of these new species in periodontal health or disease. black-pigmented anaerobes isolated from diseased and healthy subgingival sites were identified by serotyping, sds-page and physiological tests. in adult periodontitis subjects, 64% of active sites, 35.7% of inactive sites and 38.5% of healthy sites yielded black-pigmented anae ... | 1995 | 7562320 |
| in vitro activity of tetracyclines, macrolides, quinolones, clindamycin and metronidazole against periodontopathic bacteria. | we re-evaluated several antibiotics including newer ones, for their in vitro killing activity, as well as their inhibitory activity, against clinical isolates of periodontopathic bacteria. tetracyclines were active against porphyromonas gingivalis, and were highly active against prevotella intermedia, but demonstrated only a low killing activity against actinobacillus actinomycetemcomitans. rokitamycin, a new macrolide, and clindamycin were highly active against p. gingivalis and p. intermedia, ... | 1995 | 7562327 |
| occurrence of certain bacterial species and morphotypes in juvenile periodontitis in chile. | the occurrence of actinobacillus actinomycetemcomitans, porphyromonas gingivalis, prevotella intermedia, eikenella corrodens, fusobacterium nucleatum, campylobacter rectus, capnocytophaga species, and certain bacterial morphotypes was determined in 18 affected and 18 unaffected sites in 10 localized juvenile periodontitis (ljp) patients, and in 10 affected and 10 unaffected sites in 5 generalized juvenile periodontitis (gjp) patients. the subgingival proportion of the 7 bacterial species was det ... | 1995 | 7562347 |
| heat shock proteins in the human periodontal disease process. | the production of hsp by periodontopathic gram-negative bacteria was examined by sds-page, two dimensional gel electrophoresis, and western blotting using monoclonal antibodies against hsps. strains of actinobacillus actinomycetemcomitans, eikenella corrodens, fusobacterium nucleatum, prevotella intermedia, prevotella nigrescens, prevotella melaninogenica, and treponema socranskii species produced hsp which reacted with anti-yersinia enterocolitica hsp 60 and/or mycobacterial 65-kda hsp monoclon ... | 1995 | 7565172 |
| patterns of antibody response in subjects with periodontitis. | periodontal diseases comprise a heterogeneous group of infections that are difficult to distinguish on a clinical basis alone. the purpose of the present investigation was to group periodontitis subjects according to their elevated serum antibody levels to specific subgingival species. a total of 119 subjects (19-70 years) with evidence of prior periodontal destruction were monitored at 2-month intervals (maximum 8 visits), prior to therapy, using clinical parameters measured at 6 sites per toot ... | 1995 | 7567061 |
| characterization of immunoglobulin g-degrading proteases of prevotella intermedia and prevotella nigrescens. | degradation of immunoglobulins is thought to be an important factor in the causation of periodontal diseases by hindering local host defenses and by providing nutrients to the periodontal microflora. in this study, we characterized the proteolytic activity against human immunoglobulin g (igg) of 20 strains of prevotella intermedia and prevotella nigrescens isolated from periodontal pockets and oral abscesses. igg degradation was studied by sodium dodecyl sulphate-polyacrylamide gel electrophores ... | 1995 | 7567062 |
| molecular and immunological characterization of a 64-kda protein of actinobacillus actinomycetemcomitans. | the 64-kda protein to which about half the sera from patients with localized juvenile periodontitis and rapidly progressive periodontitis reacted strongly was purified from actinobacillus actinomycetemcomitans y4. determination of the n-terminal sequence of the protein revealed that it was a groel-like protein. the dna fragment containing the groel gene of a. actinomycetemcomitans was amplified by polymerase chain reaction, and the groesl operon was cloned by using colony hybridization with the ... | 1995 | 7567064 |
| [in vitro activity of carbapenems (biapenem, imipenem and meropenem) and some other antibiotics against strict anaerobic bacteria]. | during 1994, the in vitro antibiotic susceptibility of 306 anaerobic bacteria was performed in 4 hospitals, by the reference agar dilution method. among the 129 b. fragilis group strains, only two b. fragilis strains were resistant to the three carbapenems and all beta-lactams, even combined with beta-lactamase-inhibitors while metronidazole resistance could not be detected. evolution in antibiotic resistance rates could be assessed only for piperacillin whose resistance rates increased to 20%. ... | 1995 | 7567117 |
| the cellular location of prevotella ruminicola beta-1,4-d-endoglucanase and its occurrence in other strains of ruminal bacteria. | prevotella ruminicola b(1)4, tc1-1, tf1-3, and ts1-5 all produced immunologically cross-reacting 88- and 82-kda carboxymethyl cellulases (cmcases). p. ruminicola 23, 118b, 20-63, and 20-78 had much lower cmcase activities, and western blots (immunoblots) showed no cross-reaction with the b(1)4 cmcase antiserum. fibrobacter succinogenes s85 and selenomonas ruminantium hd4 and d produced cmcase, but these enzymes were smaller and did not cross-react with the b(1)4 cmcase antiserum. the b(1)4 cmcas ... | 1995 | 7574639 |
| changes in the core tonsillar bacteriology of recurrent tonsillitis: 1977-1993. | microbiological studies of the core of tonsils removed from children with recurrent tonsillitis due to group a beta-hemolytic streptococci were conducted during three periods, with 50 patients in each period: 1977-1978 (period 1), 1984-1985 (period 2), and 1992-1993 (period 3). mixed flora were present in all tonsils, with 8.1 organisms per tonsil (3.8 aerobes and 4.3 anaerobes). the predominant isolates in each period were staphylococcus aureus, moraxella catarrhalis, peptostreptococcus species ... | 1995 | 7578726 |
| effect of bacteroides melaninogenicus culture supernatant and deconjugated bile salt on lipid absorption. | lipid malabsorption is a common clinical manifestation of small bowel bacterial overgrowth. its pathogenesis, however, remains controversial. bacteroides melaninogenicus ssp. intermedius, an anaerobic bacterium, is commonly isolated from the upper bowel of patients with small intestinal bacterial overgrowth. the effects of a culture supernate of this organism and deoxycholate, an unconjugated bile salt, on intestinal oleic acid absorption were examined using a rat closed-loop model. the supernat ... | 1995 | 7587831 |
| expression of a cloned cellulase/xylanase gene from prevotella ruminicola in bacteroides vulgatus, bacteroides uniformis and prevotella ruminicola. | a new shuttle vector, prh3 (8.7 kb), was constructed for use in prevotella/bacteroides host strains. this vector combines the prri2 replicon from p. ruminicola, pbluescript sequences and a tetq marker gene for selection in prevotella/bacteroides hosts. following insertion of a fragment carrying an endoglucanase/xylanase gene from p. ruminicola 23 into the multiple cloning site, the resulting construct, prh3x, was introduced into b. vulgatus 1447, b. uniformis 1100 and p. ruminicola 2202. this re ... | 1995 | 7592134 |
| cloning, sequencing, and characterization of a membrane-associated prevotella ruminicola b(1)4 beta-glucosidase with cellodextrinase and cyanoglycosidase activities. | prevotella ruminicola b(1)4 is a gram-negative, anaerobic gastrointestinal bacterium. a 2.4-kbp chromosomal fragment from p. ruminicola encoding an 87-kda aryl-glucosidase (cdxa) with cellodextrinase activity was cloned into escherichia coli dh5 alpha and sequenced. cdxa activity was found predominantly in the membrane fraction of both p. ruminicola and e. coli, but p. ruminicola localized the protein extracellularly while e. coli did not. the hydrolase had the highest activity on cellodextrins ... | 1995 | 7592339 |
| the natural history of periodontal disease. the correlation of selected microbiological parameters with disease severity in sri lankan tea workers. | the purpose of this study was to assess the prevalence of a. actinomycetemcomitans, porphyromonas gingivalis and prevotella intermedia, and their association with periodontal disease states in a population sample from sri lanka. based on clinical parameters, a total of 536 sites in 268 male sri lankan tea workers were categorized as healthy, or showing gingivitis only, moderate or advanced periodontitis. bacterial samples were obtained from all sites and the three target bacteria identified by i ... | 1995 | 7593696 |
| cellular source, activation and inhibition of dental plaque collagenase. | dental plaque is the major aetiological factor in periodontal diseases and contains several proteolytic enzymes. the origin of these proteinases is, however, poorly studied. this study was undertaken to characterize collagenase present in dental plaque of adult periodontitis patients. vertebrate-type rather than bacterial-derived collagenase activity was detected in extracts of both supra- and subgingival dental plaque extracts of adult periodontitis patients. dental plaque collagenase was found ... | 1995 | 7593702 |
| antibacterial effects of some root end filling materials. | in addition to having good sealing ability, root end filling materials should "ideally" have some antibacterial activity to prevent bacterial growth. this investigation compared the antibacterial effects of amalgam, zinc oxide-eugenol, super eba and a mineral trioxide aggregate on nine facultative bacteria streptococcus fecalis, streptococcus mitis, streptococcus mutans, streptococcus salivarius, lactobacillus species, staphylococcus aureus, staphylococcus epidermidis, bacillus subtilis, and esc ... | 1995 | 7595152 |
| biological activities of lipopolysaccharides from oral bacteria and their relevance to the pathogenesis of chronic periodontitis. | chronic periodontitis is a major cause of tooth loss in adults and is a consequence of the colonisation of the subgingival region by organisms such as porphyromonas gingivalis, prevotella intermedia and fusobacterium nucleatum. lipopolysaccharide (lps) is a constituent of the cell walls of all of these bacteria and is found in large quantities on the surfaces of periodontally-diseased teeth. lps from oral bacteria has a marked effect on most types of cell found in the periodontal tissues includi ... | 1995 | 7597416 |
| [a study on sex hormones in gingival crevicular fluid and black pigmented bacteria in subgingival plaque of pregnant women]. | gingivitis is one of the most common oral diseases. it is caused by dental plaque and by the factors produced/released from it. the black pigmented bacteria in subgingival dental plaque are thought to be the periodontopathogens. prevotella intermedia and porphyromonas gingivalis have been shown to be closely associated with human gingivitis. prevetella intermedia can use female sex hormones such as progesterone or estradiol as a source of nutrients. in pregnant women, the concentrations of proge ... | 1995 | 7602664 |
| subgingival temperature in rats with natural gingivitis. | elevated temperature, normally a characteristic of inflammation, is a potential indicator of periodontal disease. the present investigation examined the relationship of subgingival temperature and various indicators of gingivitis in rats with spontaneous gingivitis (sus rat) and in control rats (res rat). in 24 sus rats with gingivitis, the subgingival temperature rose, but not in 6 sus and 20 res rats with healthy gingiva. subjects with high mean subgingival temperatures had significantly highe ... | 1994 | 7608849 |
| clinical features and aerobic and anaerobic microbiological characteristics of cellulitis. | to investigate the aerobic and anaerobic microbiological characteristics of cellulitis and correlate them with the infection site. | 1995 | 7611872 |
| an atypical pathway of infection in an adolescent with a deep neck space abscess. | | 1995 | 7617500 |
| correlation of the periodontal status 6 years after puberty with clinical and microbiological conditions during puberty. | the purpose of this study was to assess the oral clinical and microbiological status of young adults 6 years after puberty and to compare these findings with the conditions observed during puberty. clinical and microbiological parameters were monitored in 42 individuals 10 x between the ages of 11 and 14 years. 33 individuals were re-examined 10 years after the start of this monitoring. microbiology included 2 subgingival samples per subject taken from the mesiobuccal aspects of the upper 1st mo ... | 1995 | 7622637 |
| microbiological features of gingivitis in pubertal children. | the subgingival microflora of 42 pubertal children (aged 12-15 years) and 18 young adults (aged 21-25 years) was investigated by anaerobic culture and phase contrast microscopy. motile rods, spirochetes and prevotella intermedia were elevated proportionately in pubertal children with gingivitis (median gi > or = 1); however, no statistically significant differences in enumerated organisms on selective media were observed between pubertal children and young adults. these organisms were positively ... | 1995 | 7622639 |
| prevention of dry socket with clindamycin. a retrospective study. | clindamycin and other agents were compared for efficacy in preventing the entity "dry socket." a total 765 patients were treated with clindamycin, per os, and 408 patients were treated with other antibiotics or were non-treated controls. all patients underwent surgical removal of impacted mandibular third molars. the incidence of dry socket in untreated control and in non-clindamycin antibiotic-treated patients varied from 15 to 31 percent, while in those patients receiving clindamycin, the inci ... | 1995 | 7624100 |
| immunological detection of bacteroides fragilis in clinical samples. | a monospecific polyclonal antiserum, prepared against bacteroides fragilis common polysaccharide antigen purified by polyacrylamide gel immunoblot detected b. fragilis, b. thetaiotaomicron, b. ovatus and prevotella melaninogenica in pus samples from various anatomical sites by immunofluorescence microscopy of the pus. with standard clinical laboratory culture methods, 36% of 147 samples were positive for one or more of the above bacteria. of these, b. fragilis accounted for 33%. by immunofluores ... | 1995 | 7629860 |
| quantitative bacteriology of tonsils removed from children with tonsillitis hypertrophy and recurrent tonsillitis with and without hypertrophy. | the aerobic and anaerobic bacterial species and their numbers were studied in tonsillar specimens from children who had undergone elective tonsillectomy: 6 patients with recurrent tonsillitis (rt), 9 with recurrent tonsillitis with hypertrophy (rth), and 8 with obstructive tonsillar hypertrophy (oth). mixed flora were present in all tonsils, yielding an average of 6.7 isolates (5.6 aerobic or facultative and 1.1 anaerobic bacteria). the highest recovery rate of organisms per tonsil was in patien ... | 1995 | 7639475 |
| a defined medium for rumen bacteria and identification of strains impaired in de novo biosynthesis of certain amino acids. | a completely defined growth medium has been developed to determine the nitrogen requirements for several species of ruminal bacteria, and has revealed two strains which are impaired in de novo biosynthesis of certain amino acids. using nh4cl as a sole nitrogen source, the medium supported growth of butyrivibrio, selenomonas, prevotella and streptococcus species. one strain of b. fibrisolvens (e14) and one strain of p. ruminicola (ga33) did not grow in the presence of nh4cl until the medium was s ... | 1995 | 7639995 |
| lipid a-associated proteins from periodontopathogenic bacteria induce interleukin-6 production by human gingival fibroblasts and monocytes. | the aim of this study was to determine whether lipid a-associated proteins (lap) from two periodontopathogenic species of bacteria were able to stimulate interleukin-6 (il-6) release from human gingival fibroblasts and myelomonocytic cells. lap and lipopolysaccharide (lps) were extracted from porphyromonas gingivalis and prevotella intermedia and added to cultures of human gingival fibroblasts and mono-mac-6 monocytic cells. release of il-6 into the culture supernatants was determined by elisa. ... | 1995 | 7640674 |
| microbial flora of sinus tracts and root canals of non-vital teeth. | the occurrence of bacteria in 12 endodontically induced periodontal lesions associated with sinus tracts was examined. the microbial flora encountered in the sinus tract was compared with that of the root canal of the involved teeth which had not experienced any prior endodontic therapy. all microbiological samples taken from the sinus tract and from the root canal system contained bacteria. seventy-one strains were detected in the extraradicular lesions. of the anaerobic species, fusobacterium ... | 1995 | 7641609 |
| bacteremia in conjunction with endodontic therapy. | this study characterizes oral microorganisms believed to have spread from the root canal into the blood stream during and after endodontic therapy of teeth with asymptomatic apical periodontitis. microbiological samples were taken under aseptic conditions from the root canal of 26 single-rooted teeth in 26 patients. in the endodontic treatment of 13 of the patients (group 1), the first 3 reamers, sizes 15, 20 and 25, were used to a level 2 mm beyond the apical foramen. in the other 13 patients ( ... | 1995 | 7641631 |
| characterization of inhibitory effects of suspected periodontopathogens on osteogenesis in vitro. | by using an in vitro bone-forming culture system, the chick periosteal osteogenesis (cpo) model, the direct effects on osteogenesis of sonicated extracts derived from oral bacteria were examined. both extracts from bacterial species having strong associations with periodontal diseases (porphyromonas gingivalis, actinobacillus actinomycetemcomitans, and prevotella intermedia, hereinafter referred to as suspected periodontopathogens) and extracts from species not correlated with periodontal diseas ... | 1995 | 7642257 |
| three-year root caries incidence and risk modeling in older adults in north carolina. | the purposes of this study were to describe the incidence of root caries and to identify its risk factors in a representative sample of older adults. | 1995 | 7643330 |
| occurrence of enteric rods, staphylococci and candida in subgingival samples. | the frequency and percentage of enteric rods, staphylococci and candida were determined in 973 subgingival samples collected from 535 patients subjected to different periodontal treatment procedures. the analysis was performed with culture technique using selective and nonselective media. one or more organisms were detected in 65.5% of the samples and in 76.7% of the patients. in most samples enteric rods, staphylococci and/or candida constituted a small amount of the total microbial viable coun ... | 1995 | 7644272 |
| [comparative in vitro activity of ampicillin, cefoperazone and their combinations with sulbactam, as well as that of other antibiotics against anaerobic organisms]. | susceptibility of 180 strains of anaerobic organisms to ampicillin, cefoperazone and their combinations with sulbactam, as well as that to cefoxitin, imipenem and metronidazole was studied with the method of serial microdilutions. 100 per cent of the strains was susceptible to the ampicillin + cefoperazone combination. only 1 strain of bacteroides fragilis was resistant to imipenem. 73.4 and 57.8 per cent of the b. fragilis strains were susceptible to cefoxitin and cefoperazone respectively. no ... | 1995 | 7654095 |
| identification of proteolytic rumen bacteria isolated from new zealand cattle. | the protease activities of 212 strains of rumen bacteria isolated from new zealand cattle grazing pasture were measured. thirty-seven per cent of strains had activity greater than or equal to the proteolytic rumen bacterium prevotella ruminicola and 43 of these isolates were identified by morphology, carbon source utilization, gram stain, biochemical tests and fermentation end-product analysis. hierarchical cluster analysis showed that the strains formed four clusters: cluster a contained 26 str ... | 1995 | 7665388 |
| expression of capsules by haemophilus influenzae in mixed infections. | the pathogenicity of eight clinical isolates of non-type b haemophilus influenzae was investigated by inoculating them subcutaneously into mice, alone or mixed with viable or non-viable bacteria of certain other species. three of the h. influenzae isolates were non-capsulated while five were slightly capsulated (less than 1% of organisms had capsules). the other strains of bacteria tested were four isolates of capsulated and four isolates of non-capsulated pigmented strains of prevotella sp. and ... | 1995 | 7673745 |
| delmopinol interactions with cell walls of gram-negative and gram-positive oral bacteria. | the main purpose of the present study was to investigate the influence of delmopinol hydrochloride on the cell surface morphology of gram-negative and gram-positive bacterial cells by using transmission electron microscopy. a second purpose was to evaluate the extraction of cell wall material caused by delmopinol and the binding of radiolabelled delmopinol to the various strains. fresh isolates and type strains of gram-negative rods associated with periodontal disease, porphyromonas gingivalis, ... | 1995 | 7675514 |
| detection of tet(m) and tet(o) using the polymerase chain reaction in bacteria isolated from patients with periodontal disease. | the polymerase chain reaction was used to examine 114 tetracycline-resistant anaerobic and facultative anaerobic bacterial isolates from patients with periodontal disease for the tet(m) and tet(o) genes. a 740-base-pair fragment of the tet(m) gene was amplified from 84 of 114 isolates, and a 519-base-pair fragment of the tet(o) gene was amplified from 13 streptococcal isolates. six of 7 tetracycline-resistant isolates of veillonella spp. and tetracycline-resistant isolates of eubacterium spp. (n ... | 1995 | 7675524 |
| non-radioactively labelled dna probes for the detection of periodontopathogenic prevotella and porphyromonas species. | digoxigenin-labelled synthetic dna probes directed against the 16s rrna were used for the direct detection of the periodontopathogenic bacteria prevotella intermedia and porphyromonas gingivalis in subgingival plaque by applying a dna-rna dot-blot hybridization procedure. the test was evaluated with 134 plaque samples from 26 patients with adult periodontitis or rapidly progressive periodontitis. the lower limit of detection was 10(4)-10(5) bacteria/specimen. a semiquantitative assessment of the ... | 1993 | 7686072 |
| ecophysiology and taxonomy of bacteroides and related taxa. | recent taxonomic changes among bacteroides and related species has led to a clearer insight into the site specificity of these taxa. two recently described centers of variation within the genus bacteroides sensu stricto and prevotella intermedia are reported. however, considerable taxonomic problems still remain unresolved within this large and complex group of microorganisms. these bacteria are metabolically diverse, and this is reflected in their colonization sites. in the rumen, gastrointesti ... | 1993 | 7686781 |
| the in vitro uptake and metabolism of peptides and amino acids by five species of rumen bacteria. | streptococcus bovis jb1, prevotella ruminicola b(1)4, selenomonas ruminantium z108, fibrobacter succinogenes s85 and anaerovibrio lipolytica 5s were incubated with either 14c-peptides (mol. wt, 200-1000) or 14c-amino acids to compare their rates of uptake and metabolism. in experiment 1, the bacteria were grown and incubated in a complex medium, but no uptake of 14c-labelled substrates occurred. when casein digest was omitted, uptake rates of 14c-peptides were different (p < 0.01) with each spec ... | 1995 | 7698948 |