| modulation of macrophage hyperactivity improves survival in a burn-sepsis model. | macrophage hyperactivity with increased production of tumor necrosis factor, interleukin 6, interleukin 1, and prostaglandins has been demonstrated in the injured patient, but the effect of this on the clinical outcome is unclear. we studied the effect of combination interleukin 1 beta and indomethacin sodium therapy on macrophage hyperactivity and survival after sepsis in a murine burn model. macrophage interleukin 1, interleukin 6, and tumor necrosis factor alpha production were all significan ... | 1992 | 1540091 |
| chronic coxiella burnetii infection mimicking malignant hematologic disease. | | 1992 | 1553961 |
| [new possibilities for the diagnosis of q fever and for the differentiation of the causative agent]. | the current diagnostic procedures for q fever are discussed, especially isolation and propagation of coxiella burnetii in bgm cell cultures, detection of antigen by an enzyme immunoassay using a biotinylated monoclonal antibody and the polymerase chain reaction (pcr). the latter is of special importance for differentiation of isolates. | 1992 | 1558529 |
| fine-needle aspiration findings of the liver in a case of q fever. | this report describes the cytologic findings in q fever involving the liver in a 59-yr-old man. the fine-needle aspiration findings are correlated with a surgical needle biopsy performed concurrently. cytopathologists should include q fever in their differential diagnosis of granulomatous inflammation. ring granulomas, which can be seen in an aspiration biopsy, should suggest the diagnosis of q fever. | 1992 | 1568415 |
| b-cell factor 1 is required for optimal expression of the dra promoter in b cells. | the x box in the dra promoter of the human histocompatibility complex is required for expression of the dra gene in b cells. we show that a b-cell factor binds to a sequence that is clearly distinguishable from binding sites for the previously described x box binding nuclear proteins rf-x, nf-x, nf-xc, nf-s, hxbp, and ap-1. mutations in the dra x box that disrupt the binding of this factor result in a lower level of gene expression, as does the presence of id (a trans-dominant regulatory protein ... | 1992 | 1569956 |
| immature transformed rat islet beta-cells differentially express c-peptides derived from the genes coding for insulin i and ii as well as a transfected human insulin gene. | synthetic peptides representing unique sequences in rat proinsulin c-peptide i and ii were used to generate highly specific antisera, which, when applied on sections of normal rat pancreas, confirm a homogeneous coexpression of the two c-peptides in all islet beta-cells. insulin gene expression is induced in the transformed heterogeneous rat islet cell clone, nhi-6f, by transient in vivo passage. during this process a transfected human insulin gene is coactivated with the endogenous nonallelic r ... | 1992 | 1569972 |
| acute and chronic q fever in patients with cancer. | q fever is caused by coxiella burnetii, a strictly intracellular bacterium that lives within the phagolysosome of infected cells. we report here five cases of q fever in patients with cancer. three of them had a solid tumor, one had a b cell lymphoma, and one had chronic myeloid leukemia. one patient had acute q fever, and the four others had chronic q fever endocarditis. two patients with endocarditis had no previous history of valvulopathy. c. burnetii was isolated from the valves of two patie ... | 1992 | 1571416 |
| immunoblot technique for q fever (technical note). | partially purified but unfixed c. burnetii cells were submitted to sodium dodecylsulfate polyacrylamide gel electrophoresis, western blotted and used as antigens for evaluation of antibody responses against the agent in mice, cattle and humans. increased specificity was achieved by using glutaraldehyde as additional blocking agent. gels as well as membranes were subjected to laser densitometry and data analysed with an ibm personal computer. this technique offers the possibility to objectively q ... | 1992 | 1572416 |
| [neurologic complications of q-fever (coxiella burnetii)]. | we report on fifteen patients with a typical syndrome of q-fever. four patients (= 27%) showed signs of nervous system involvement. one patient developed a meningitic syndrome, another patient had a passing psychosis. in two patients, severe cerebellar deficits were predominant. it is pointed out that these two patients were under lithium therapy because of manic-depressive illness. a synergetic neurotoxic effect of cociella burneti infection and lithium is discussed as a possible cause of the s ... | 1992 | 1577359 |
| [q fever endemic in the palatinate]. | in 1989 investigations revealed a small scale outbreak of q fever in the area of vorderpfalz several kilometers west of the city of ludwigshafen/rhein. in 16 cases the diagnosis of q fever has been serologically confirmed by complement fixation test (cf), the estimated number of unknown cases, however, may be considerable. a flock of sheep was determined as the source of infection. due to the prevalence of the tick species dermacentor marginatus, one of the main vectors of the infection, the are ... | 1992 | 1579564 |
| [acute meningoencephalitis caused by coxiella burnetii with periodic eeg complexes]. | q fever is a zoonosis found worldwide and is produced by coxiella burnetii. it may be acute or chronic with neurological manifestations being infrequent. several cases of acute encephalitis or meningoencephalitis have been described, generally with an evolution towards cure regardless of the use of selective antibiotic treatment. recently the authors had the opportunity to study a 33 year old male presenting acute meningoencephalitis in which the clinical manifestations, csf findings (increase i ... | 1992 | 1596401 |
| catheter infection caused by methylobacterium in immunocompromised hosts: report of three cases and review of the literature. | three cases of catheter infection due to methylobacterium extorquens are reported. each patient had a history of acute leukemia and was immunocompromised; two had undergone bone marrow transplantation, and the third was receiving consolidation chemotherapy. all three patients survived after removal of the central venous catheter and antibiotic treatment. the clinical features of these cases are compared with those of the 12 previously reported cases of infection due to methylobacterium species. | 1992 | 1600002 |
| [severe meningoencephalitis in acute coxiella burnetii infection]. | | 1992 | 1605464 |
| [rational means of the determination of costs and etiological diagnosis of community-acquired pneumonia]. | the cost and effectiveness of examinations (sputum staining and culturing, antitest determination for influenza a and b, rsv, adenovirus, chlamydia psittaci and pneumoniae, coxiella burnetii, mycoplasma pneumoniae and legionella pneumophila, and determination for streptococcus pneumoniae antigen) performed to explore the aetiology of community-acquired pneumonia in the case of 258 hospitalised patients were analysed. the aetiology could be determined in 44.2% of the cases. on the basis of prevai ... | 1992 | 1608606 |
| association of the type ii camp-dependent protein kinase with a human thyroid rii-anchoring protein. cloning and characterization of the rii-binding domain. | the type ii camp-dependent protein kinase (pka) is localized to specific subcellular environments through binding of the dimeric regulatory subunit (rii) to anchoring proteins. subcellular localization is likely to influence which substrates are most accessible to the catalytic subunit upon activation. we have previously shown that the rii-binding domains of four anchoring proteins contain sequences which exhibit a high probability of amphipathic helix formation (carr, d. w., stofko-hahn, r. e., ... | 1992 | 1618839 |
| evaluation of a competitive enzyme immunoassay for detection of coxiella burnetii antibody in animal sera. | a competitive enzyme immunoassay (ceia) was established and compared with other serological techniques for detecting coxiella burnetii antibody in camels, goats, and sheep. this technique was evaluated because a conjugated anti-camel immunoglobulin was not available to serve as a direct signal for the demonstration of antigen-antibody reaction. a c. burnetii antibody-positive human serum and a peroxidase-conjugated anti-human immunoglobulin g were used as an indicator system competing against an ... | 1992 | 1624582 |
| vaccines against coxiellosis and q fever. development of a chloroform:methanol residue subunit of phase i coxiella burnetti for the immunization of animals. | we have demonstrated the safety, immunogenicity, and efficacy of the wc and cmr vaccines in guinea pigs. vaccination of guinea pigs with either wc or cmr protects animals against challenge with virulent c. burnetii. a total of 2 micrograms of either wc or cmr vaccine was a significant priming dose. a total of 20 micrograms gave complete protection against lethal challenge. detection of antibodies to phase ii cells by microaglutination, after vaccination with either wc or cmr and before lethal ch ... | 1992 | 1626897 |
| technetium-99m p-iodophenethyldiaminodithiol (dadt-ipe): potential brain perfusion imaging agent for spect. | a new ligand, an n-p-iodophenethyl diaminodithiol (dadt-ipe), an anlog of n-isopropyl-p-iodoamphetamine (imp), was synthesized and subsequently complexed with 99mtc, using stannous chloride as a reducing agent. two complexes (a and b) were separated from 99mtc-dadt-ipe by high performance liquid chromatography (hplc). competitive inhibition studies showed that the ic50 value of dadt-ipe (70 microm) was similar to that of imp (49 microm). biodistribution studies of one of the complexes [99mtc-dad ... | 1992 | 1629020 |
| [coxiella burnetii osteoarthritis]. | a case of a 25-year-old female characterized by febrile osteoarthritis and arthromyalgias as the only clinical manifestation of a q fever is discussed. analytical data were compatible with those of an acute inflammation and serologic findings reached diagnostic levels. response to doxycycline treatment was totally satisfactory. even though associated arthromyalgias are described in some cases of q fever as well as sporadic cases of osteoarthal infection due to coxiella burnetii, there are no oli ... | 1992 | 1631356 |
| influence of a menhaden oil diet on cercarial penetration of schistosoma mansoni. | the ability of a menhaden oil (mo) diet to influence cercarial penetration into mouse tail skin was evaluated. male cd-1 mice 4-6 wk old (15.2 g average weight) were fed a 0, 10%, or 20% mo-supplemented diet for 2 wk. after this time mice were infected with either 65 +/- 3 or 145 +/- 3 [35s]methionine/cysteine-labeled cercariae for 1 hr by tail immersion. twenty-four hours and 7 days later groups of mice were killed and their tail skin removed and autoradiographed. at 24 hr postinfection, mice f ... | 1992 | 1635035 |
| a cluster of coxiella burnetii infections associated with exposure to vaccinated goats and their unpasteurized dairy products. | an outbreak of q fever occurred among patients and staff of a psychiatric institution in southern france. some of the patients and staff left the institution daily to work on a farm where goats were raised for raw milk and cheese production. the goats had all been vaccinated annually with a commercial vaccine containing phase ii coxiella burnetii antigen. a serologic survey revealed that 40 (66%) of the 61 patients and staff had elevated titers to c. burnetii. seropositive persons were more like ... | 1992 | 1636881 |
| [coxiella burnetii and chlamydia psittaci infection in dogs]. | the prevalence of coxiella burnetii and chlamydia psittaci antibodies was investigated in 530 dog specimens divided into six groups, i. e. a = private watch dogs, b1 = service dogs from bratislava, b2 = service dogs from other localities of slovakia and moravia, c = watch dogs from farms, i = household dogs, t = stray dogs. the dogs demonstrated the higher seropositivity to c. burnetii (11.7%) than to ch. psittaci (5.5%). the highest percentage of antibodies to c. burnetii was found in stray dog ... | 1992 | 1641947 |
| the pharmacokinetics and biodistribution of technetium-99m(v)dimercaptosuccinic acid in an animal tumor model. | this study used an established rabbit tumor model with squamous carcinoma to evaluate the pharmacokinetics and biodistribution of technetium-99m-(v)dimercaptosuccinic acid. a total of 54 rabbits were studied (25 with no tumor; 29 with tumor). technetium-99m(v)dimercaptosuccinic acid had a bi-exponential blood clearance in rabbits with no tumors (28 and 325 min) and in rabbits with tumors (27 and 352 min). there was no significant difference (p greater than 0.05) in mean clearance times between t ... | 1991 | 1646304 |
| sero-prevalences of selected cattle diseases in the kafue flats of zambia. | sera from five traditionally managed herds grazing in the kafue flats were tested for antibodies to bovine viral diarrhoea-mucosal disease (bvd-md), parainfluenza 3 (pi3), infectious bovine rhinotracheitis-infectious pustular vulvovaginitis (ibr-ipv), bovine adenovirus 3 (bav3) and bluetongue (bt). the sero-prevalences of the first four diseases were respectively 76.2, 94.4, 42.1 and 87.4%. five samples (2.3%) gave doubtful reactions for bt. prevalences of 28.5% for brucellosis, 14% for rift val ... | 1991 | 1646515 |
| q fever: from deer to dog to man. | | 1991 | 1672016 |
| genetic mapping of new rflps at xq27-q28. | the development of the human gene map in the region of the fragile x mutation (fraxa) at xq27 has been hampered by a lack of closely linked polymorphic loci. the polymorphic loci dxs369 (detected by probe rn1), dxs296 (vk21a, vk21c), and dxs304 (u6.2) have recently been mapped to within 5 cm of fraxa. the order of loci near fraxa has been defined on the basis of physical mapping studies as cen-f9-dxs105-dxs98-dxs369-dxs297-fraxa-++ +dxs296-ids-dxs304-dxs52-qter. the probe vk23b detected hindiii ... | 1991 | 1672291 |
| differentiation of coxiella burnetii isolates by analysis of restriction-endonuclease-digested dna separated by sds-page. | thirty-two isolates of coxiella burnetii collected from various hosts ranging from arthropods to man were compared by restriction endonuclease (re) digestion patterns of chromosomal dna using sds-page. sds-page provided better dna fragment separation than agarose gel electrophoresis and enabled the differentiation of these isolates into six distinct groups on the basis of dna restriction fingerprints. two groups of chronic disease isolates could be distinguished, each having unique re digestion ... | 1991 | 1673152 |
| infection of aortic aneurysm with coxiella burnetii. | | 1991 | 1678848 |
| differential ontogenetic expression and regulation of proenkephalin and preprosomatostatin mrnas in rat caudate-putamen as studied by in situ hybridization histochemistry. | specific oligonucleotide probes and in situ hybridization histochemistry were used to study the ontogeny and regulation of the mrnas for proenkephalin a and preprosomatostatin in rat brain. in adult brain the most intense hybridization signal for the proenkephalin a mrna was in caudate putamen, nucleus accumbens and olfactory tubercle. by contrast, the hybridization signal for preprosomatostatin mrna was more diffusely scattered throughout the brain, with high signals in the neocortex, olfactory ... | 1991 | 1680033 |
| hemodynamic responses to alpha-adrenergic blockade during hypoxemia in the fetal lamb. | the mechanisms responsible for the increase in umbilical venous and hepatic vascular resistance during hypoxemia are poorly understood. to assess the relative importance of alpha-adrenergic receptors, we produced an acute, severe hypoxemia in chronically instrumented fetal sheep. while fetal arterial oxygen saturation was maintained at the same level, we then injected phentolamine, a selective alpha-adrenoreceptor blocker. we found that the hypoxemia-induced vasoconstriction of the umbilical vei ... | 1991 | 1686269 |
| analysis of strain-specific plasmid sequences from coxiella burnetii. | acute isolates of coxiella burnetii possess a 36-kbp plasmid termed qph1. dna hybridizations show that qph1 contains approximately 6-kbp region of dna which is not present in the qprs plasmid from chronic isolates. this qph1-specific region of dna contains the contiguous ecori fragments g, e, and d. the ged region was found to possess seven open reading frames (orf's) coding for proteins ranging from 5.5 to 42.3 kda in molecular mass when subcloned and expressed in vitro. summing the predicted o ... | 1991 | 1687630 |
| analysis of coxiella burnetii isolates in cell culture and the expression of parasite-specific antigens on the host membrane surface. | coxiella burnetii isolates may be classified into several groups based on plasmid character. these groups may also be correlated with disease syndrome--chronic or short-term acute. l929 mouse fibroblast cells were exposed, independently, to two members of the three major c. burnetii groups, and their growth/morphological characteristics analysed by light and electron microscopy, including high voltage electron microscopy. the fates of the isolates were followed. two acute isolates [nine mile (rs ... | 1991 | 1687632 |
| physical mapping of the coxiella burnetii genome. | coxiella burnetii isolates from different genomic groups contain restriction fragment polymorphisms that were easily distinguishable using pulsed field gradient electrophoresis (pfge). conversely, isolates that belong to the same genomic group yield identical patterns indicating that pfge can be used to identify the genomic grouping of new c. burnetii isolates. intact c. burnetii cells were embedded in agarose and lysed in situ. the genomic dna was digested with low-frequency cutting restriction ... | 1991 | 1687633 |
| evidence for the structural heterogeneity of the polysaccharide component of coxiella burnetii strain nine mile lipopolysaccharide. | highly purified lipopolysaccharide (lps) preparation obtained from coxiella burnetii strain nine mile in phase i was used to determine the structure and monosaccharide composition of the polysaccharide component. the procedure included sodium dodecyl sulphate-polyacrylamide gel electrophoresis followed by silver staining and gel chromatographic fractionation of acetic acid-hydrolyzed lps. five fractions (a-e) were analysed by glc-mass spectrometry. d-mannose and d-glycero-d-mannoheptose were pre ... | 1991 | 1687636 |
| analysis of proteins and lipopolysaccharides from chinese isolates of coxiella burnetii with monoclonal antibodies. | four coxiella burnetii isolates in china and two reference strains were compared by sds-page and immunoblotting. the sds-page profiles of whole cells and lps of chinese isolates qiyi, xinqiao, and ys-8 were found closely related to henzerling strain, and different from the grita strain. in immunoblot assay of lps and proteinase k-digested whole rickettsiae minor differences were seen in polysaccharide structure among the chinese isolates by phase i monoclonal antibody. the present results sugges ... | 1991 | 1687637 |
| electron microscopy of coxiella burnetii in tissue culture. induction of cell types as products of developmental cycle. | an in vitro ultrastructural study was carried out on tissue cultures (j774, murine macrophage-like tumour cell line, and bhk-21, baby hamster kidney cell line) persistently infected with c. burnetii to investigate whether the events of cellular differentiation could be visualized. at a given stage of the developmental cycle, a proportion of the cells within the affected phagolysosomes clearly underwent cellular differentiation. the cells initially showed asymmetrical septation, the primary stage ... | 1991 | 1687638 |
| coxiella and rickettsiella: comparison of ultrastructure with special reference to their envelope. | ultrastructure of rickettsiella phytoseiuli (r.p.) multiplying in female ticks dermacentor reticulatus was compared with that of coxiella burnetii (c.b.) in the same ticks and in mice. c.b. in ticks and mice were always represented by 2 main cell types: small dense round or rod-like cells (dc) and larger bacteria-like cells (bc). dc were surrounded with a characteristic five-layered 20 nm thick envelope. under the envelope dc had a stack of parallel intracytoplasmic membranes with a periodicity ... | 1991 | 1687641 |
| effects of cytokines on prostaglandin e and camp levels in human periodontal ligament fibroblasts in vitro. | the stimulation of pge synthesis and camp production by cytokines have important physiological effects in many target tissues. the effects of interleukin-1 alpha and -1 beta, tumour necrosis factor-alpha and interferon-gamma on pge and camp production by periodontal ligament fibroblasts were studied. fibroblasts in the 4th-6th passage, grown and maintained in dmem supplemented with 10% equine serum, were incubated with graded doses of the various cytokines for 0.25, 0.5, 1.2, 4, 24, 48 or 72 h. ... | 1990 | 1695503 |
| projections of substance p, vasoactive intestinal peptide and tyrosine hydroxylase immunoreactive nerve fibres in the canine intestine, with special reference to the innervation of the circular muscle. | antisera raised against neuron specific enolase (nse), substance p, vasoactive intestinal peptide (vip) and tyrosine hydroxylase (th) were used to reveal nerve fibres in the wall of the canine small and large intestine. the circular muscle of the colon was innervated by nerve fibre bundles that ran parallel to the muscle throughout its thickness. a plexus of fibre bundles was found against the inner (submucosal) surface of the circular muscle. fibres with substance p, vip and th immunoreactivity ... | 1990 | 1695512 |
| injection of inactivated phase i coxiella burnetii increases non-specific resistance to infection and stimulates lymphokine production in mice. | the encounter of phase i c. burnetii with the host results in seemingly disparate consequences. on the one hand, in vitro lymphocyte responses to mitogens and homologous recall antigen are suppressed. on the other, host resistance to a variety of infectious agents and to a tumor is increased. an explanation for this augmented immune response surely involves the ability of c. burnetii to stimulate cytokines, such as interferon and tnf, which enhance host immune function. | 1990 | 1696075 |
| iloprost inhibits neutrophil-induced lung injury and neutrophil adherence to endothelial monolayers. | we hypothesized that iloprost, a long-acting prostacyclin analog, would inhibit neutrophil (pmn)-induced lung injury and decrease pmn adherence to vascular endothelium. human pmns infused into isolated buffer-perfused rat lungs subsequently stimulated with phorbol myristate acetate (pma) resulted in lung injury as assessed by the accumulation of [125i]bovine serum albumin (125i-bsa) in lung parenchyma and alveolar lavage fluid. addition of iloprost to the lung perfusate, prior to activation of t ... | 1990 | 1698399 |
| conduction velocity distribution of afferent fibers innervating the rat urinary bladder. | the conduction velocities of individual afferent fibers innervating the rat urinary bladder were determined by the antidromic stimulation of dorsal roots while recording from bladder postganglionic nerves. conduction velocities ranged from 0.5 to 21.0 m/s; 70% of the velocities were less than 2.5 m/s. the distribution within the dorsal roots was ipsilateral with 84% in l6 and 16% in s1. neuroanatomical tracing with horseradish peroxidase applied to individual bladder postganglionic nerves result ... | 1990 | 1698508 |
| coxiella burnetii antigens may induce resistance to tumour growth. | coxiella burnetii antigens stimulate the defence against growth of hepatoma 22a cells. the antigen-stimulated mice survived longer, they considerably later developed palpable tumours and showed a retarded tumour growth. the enhanced resistance to tumour growth may be explained by at least 2 interrelated phenomena; namely by the induction of interferon-like activity and an increased nk cell activity. | 1990 | 1705746 |
| antigenic differences between coxiella burnetii cells revealed by postembedding immunoelectron microscopy and immunoblotting. | the aim of this study was to investigate the antigenic structures of the morphologically distinct cells of the coxiella burnetii developmental cycle. postembedding immunoelectron microscopy with polyclonal antibodies produced in rabbits to (i) phase i cells, (ii) a chloroform-methanol residue fraction of cells, (iii) the cell walls (cw) of large and small cells and small dense cells (sdc), and (iv) the peptidoglycan-protein complexes of small cells and sdc labelled the continuum of morphological ... | 1991 | 1715326 |
| pneumonia. patient profiles, choice of empiric therapy, and the place of third-generation cephalosporins. | choosing appropriate antimicrobial therapy for patients with pneumonia requires knowledge of the etiologic agents seen in specific kinds of patients at specific times and places. for community-acquired pneumonia, there is an important difference in the agents seen in the normal and the compromised host. the normal host most often presents with viral, mycoplasmal, or pneumococcal pneumonia. the exact place of chlamydia pneumoniae is still under study. a normal host who aspirates is at risk of ana ... | 1992 | 1730186 |
| sequence and linkage analysis of the coxiella burnetii citrate synthase-encoding gene. | the nucleotide (nt) sequence of the coxiella burnetii citrate synthase-encoding gene (glta), previously cloned in escherichia coli, was determined. the nt sequence analysis revealed an open reading frame (orf) of 1290 bp capable of coding for a protein of 430 amino acids (aa) with a deduced mr of 48,633. preceding an atg start codon, a possible transcription start point (tsp) with homology to the e. coli promoter consensus was detected. a poly-purine-rich region occurred immediately upstream fro ... | 1991 | 1756983 |
| [the significance of sheep and goats as carriers of zoonoses in this country]. | the significance of sheep and goats in this country in connection with the zoonoses rabies, tick-borne encephalitis, contagious ecthyma, q-fever, chlamydiosis, brucellosis, campylobacteriosis, echinococcosis and toxoplasmosis is discussed. | 1991 | 1759259 |
| [the host reaction to the administration of different components of coxiella burnetii]. | after the intraperitoneal injection of corpuscles of c. burnetii antigen (ag), phospholipid (pl), and sediment obtained after the extraction of pl from ag with chloroform-methanol (cm) slight leukocytic reaction developed in the peritoneum on day 1, and on day 2 it could be observed in the liver and in the spleen. ag induced the most pronounced morphological changes. in the spleen they were manifested by the activation of t- and b-dependent zones of white pulp from day 2 and by the pronounced hy ... | 1991 | 1759530 |
| shell-vial assay: evaluation of a new technique for determining antibiotic susceptibility, tested in 13 isolates of coxiella burnetii. | coxiella burnetii is a strictly intracellular bacterium. bacteriostatic effects have been described previously on a few isolates in embryonated eggs (a. j. spicer, m. g. peacock, and j. c. williams, p. 375-383, in w. burgdorfer and r. l. anacker, ed., rickettsiae and rickettsial diseases, 1981). we used the shell-vial technique (d. raoult, g. vestris, and m. enea, j. clin. microbiol. 28:2482-2484, 1990) to determine the susceptibility of c. burnetii to amoxicillin, amikacin, erythromycin, co-tri ... | 1991 | 1759829 |
| analysis of high psa n-cam expression during mammalian spinal cord and peripheral nervous system development. | using a monoclonal antibody that recognizes specifically a high polysialylated form of n-cam (high psa n-cam), the temporal and spatial expression of this molecule was studied in developing spinal cord and neural crest derivatives of mouse truncal region. temporal expression was analyzed on immunoblots of spinal cord and dorsal root ganglia (drgs) extracts microdissected at different developmental stages. analysis of the ratio of high psa n-cam to total n-cam indicated that sialylation and desia ... | 1991 | 1769342 |
| coronary artery thrombosis and thrombolysis in baboons: the effect of atenolol treatment on myocardial infarct size. | the effect of the beta-blocker atenolol on experimental infarct size was studied in a non-human primate model. in 12 baboons thrombosis of the left anterior descending coronary artery (lad) was induced and atenolol (0.1 to 0.2 mg.kg-1 intravenously, sufficient to lower the heart rate by 20%) was administered 10 min after the onset of ischaemia in six animals, whereas the others received placebo. thrombolysis was induced 60 min after the onset of ischaemia by intravenous injection of rtpa (12 mic ... | 1991 | 1782934 |
| [in vivo and in vitro growth of the tk-1 strain of coxiella burnetii]. | all a/j, balb/c and c57bl/6 murine strains were resistant against the intraperitoneal infection with tk-1 strain, but an enhancement of susceptibility of mice were revealed by the administration of cyclophosphamide (cpa) in balb/c and a/j strains. cpa-treated balb/c mice allowed an increase of tk-1 strain up to 1.7 x 10(6) coxiellar particles/mg spleen. but athymic nude mice of balb/c strain showed only a slight increase of coxiellar particles in spleen. the resident peritoneal macrophages from ... | 1991 | 1783802 |
| serological evidence that the q fever agent (coxiella burnetii) has spread widely among dairy cattle of japan. | serological examination of bovine and human sera for antibodies against coxiella burnetti was carried out by the immunofluorescence technique. twenty to 30% of the cows examined were antibody-positive. sera from two veterinarians also had antibody against c. burnetii. these results suggest an increase in the number of infected cows with c. burnetii in japan since 1954, and also imply the possibility of the prevalence of acute q fever in the human population, which had been underestimated and und ... | 1991 | 1784259 |
| inhibition of glycemic and hormonal responses after repetitive sucrose and starch loads by different doses of the alpha-glucosidase inhibitor miglitol (bay m 1099) in man. | in two randomized, placebo-controlled, double-blind studies, the efficacy, duration of action and tolerability of a single morning dose of 25, 50, and 100 mg miglitol (bay m 1099), an absorbable inhibitor of intestinal alpha-glucosidases, were assessed after repetitive sucrose or maize-starch loads (50 g of carbohydrates in 400 ml of water each at 08.00, 12.00, and 17.00 h). with sucrose, miglitol reduced the postprandial rise in blood glucose, serum insulin and serum gastric inhibitory polypept ... | 1991 | 1784628 |
| [the pathogenesis of q fever in the intratracheal infection of guinea pigs]. | the intratracheal introduction of r. burnetii is the method of infection, highly similar to aerogenic infection, the most widespread route of r. burnetii infection in humans under natural conditions. this suggests that the study of the pathogenesis of q fever resulting from the intratracheal infection of guinea pigs is highly topical. the authors have established that after the penetration of the infective agent generalized infection develops in the animals with rickettsiae accumulating mostly i ... | 1991 | 1789036 |
| demyelinating polyradiculoneuritis following coxiella burnetti infection (q fever). | neurological complications of coxiella burnetii infection (q fever) are rare, although the occurrence of headache, paresthesias, and transient focal deficits has been reported. we report the case of a patient with a relapsing demyelinating polyradiculoneuritis as an aftermath of c. burnetti endocarditis and pneumonia. | 1991 | 1791136 |
| [q fever and fetal death in utero. two cases]. | some cases of late abortion occurring after a coxiella burneti infection, more often with a chronic evolution, have already been mentioned in the literature. we reported here two cases of early abortion, contemporaneous of an acute infection due to c. burneti. two patients, after a contact, before and at the beginning of the pregnancy, with an animal susceptible to contaminate human beings by c. burneti, presented no clinical symptom characteristic of q fever. the fetal death for the two cases w ... | 1991 | 1791290 |
| [application of trichloroacetic acid-treated antigen for serodiagnosis of q fever by indirect immunofluorescence technique]. | it is well known that the etiologic agent, coxiella burnetii, exhibits an antigenic phase variation (phase i to phase ii), and the diagnostic significance of the relative antibody titers against phase i and phase ii antigens is pointed out. therefore both phase i and phase ii antigens are necessary for the serological examination of q fever. but it is not so easy to prepare and maintain the phase ii antigen by the conventional method. in the present study we tried to prepare the phase ii antigen ... | 1991 | 1791325 |
| [enzootic abortion in a goat herd, caused by mixed infection with coxiella burnetii and chlamydia psittaci. case report]. | we report on an outbreak of enzootic abortion in a herd of goats with high losses caused by mixed infection with coxiella burnetii and chlamydia psittaci. both agents could be detected by microscopic investigation of smears from afterbirths by giménez staining and by a capture enzyme-linked immunofluorescence assay (capture elifa). additionally, coxiella burnetii was isolated from the same tissues in bgm cell cultures. serological investigations with cf, indirect elifa, and if tests revealed hig ... | 1991 | 1796465 |
| a primary demyelinating disorder of young cattle. | the clinical signs and neuropathological changes are described in six limousin x calves that, at about one month after birth, showed signs of blindness, nystagmus, rotation of the eyes, opisthotonos, hyperprotraction of the forelegs and, in one case, apparent seizures. pathologically there was necrosis of the optic chiasma and focal areas of myelin sheath vacuolation or demyelination in certain areas of the brain, especially in the cerebellar peduncles. in one animal, kept alive for 7 months, th ... | 1991 | 1800911 |
| [a trial of cultivating rickettsiella phytoseiuli on the sm imv-72 medium used for growing phytopathogenic mycoplasmas]. | the work presents data on growing rickettsiella phytoseiuli in the sm imv-72 medium which is used for cultivation of phytopathogenic mycoplasmas. rickettsiella was observed till the 66th day in the primary cultures and till the 16th day after the first passage. binary division of the cells has been found only in the primary cultures; a complex cycle of the rickettsiella development ceased at the stage of formation of the crystal-forming cells. attempts to passivate rickettsiella were failure. | 1991 | 1819757 |
| [evaluation of the sensitivity of selected serological tests and activity of coxiella burnetii antigens in the diagnosis of q fever]. | sensitivity of three serological tests: indirect immunofluorescence assay (if), complement fixation test (cf), and microagglutination test (ma) was evaluated. sera (118 samples) of humans suspected of c. burnetii infection were tested. phase ii antibodies were detected in 68.6% of sera and phase i antibodies--in 38.2% of sera. among seropositive to phase ii antigen--93.8% of sera reacted in if, 62.9% in ma, and 32.1% in cf; among seropositive to phase i antigens--100% of samples reacted in if, 2 ... | 1991 | 1823374 |
| stage of investigations on ixodides, parasites of small mammals and nematocera. | | 1991 | 1823495 |
| expression of isoforms of internal ca2+ pump in cardiac, smooth muscle and non-muscle tissues. | smooth muscle and several non-muscle tissues contain mrna for an alternative splice of the mrna for the cardiac sarcoplasmic reticulum (sr) ca,mg-atpase. based on amino acid composition deduced from cdna sequences the cardiac isoform (ic) is 110 kda while the smooth muscle and the non-muscle isoform (is) is 115 kda. this prediction in their molecular masses was tested at the protein level in rabbit stomach, aorta, uterus and vas deferens smooth muscles; stomach mucosa, brain, liver, kidney and h ... | 1991 | 1826611 |
| [livedo and circulating anticoagulants in q fever]. | | 1991 | 1827908 |
| [inhibitor effect of cd8+cd57+ lymphocytes on cell-mediated cytotoxicity: characterization of suppressor factor]. | we report a new suppressor function of cd8+cd57+ lymphocytes from hiv-seropositive patients or allotransplanted recipients, on the cytolytic activity of allospecific ctl, nk and lak cells. this inhibitory effect is mediated by a non-antigen specific soluble factor distinct from pge2, tgf beta and tnf alpha beta. a preliminary biochemical characterization indicates that the cd8+cd57+ inhibitory activity 1. is heat and trypsin resistant, 2. specifically binds to concanavalin a suggesting its glyco ... | 1991 | 1828385 |
| [inhibitor effect of cd8+cd57+ lymphocytes on cell-mediated cytotoxicity: characterization of suppressor factor]. | we report a new suppressor function of cd8+cd57+ lymphocytes from hiv-seropositive patients or allotransplanted recipients, on the cytolytic activity of allospecific ctl, nk and lak cells. this inhibitory effect is mediated by a non-antigen specific soluble factor distinct from pge2, tgf beta and tnf alpha beta. a preliminary biochemical characterization indicates that the cd8+cd57+ inhibitory activity 1. is heat and trypsin resistant, 2. specifically binds to concanavalin a suggesting its glyco ... | 1991 | 1828385 |
| tissue distribution of restricted leukocyte common antigens. a comprehensive study with protein- and carbohydrate-specific cd45r antibodies. | a panel of monoclonal antibodies, specific for either protein or carbohydrate components of the leukocyte common antigen (lca), were used to characterize the expression pattern of different variants of this antigen complex. immunohistochemical and flow cytometric analysis was carried out on human lymphoid tissues from different anatomical compartments. we found that variations in terminal sialic acid or o-linked sugar moieties were associated with drastic differences in reactivity with some of t ... | 1991 | 1828514 |
| tissue distribution of restricted leukocyte common antigens. a comprehensive study with protein- and carbohydrate-specific cd45r antibodies. | a panel of monoclonal antibodies, specific for either protein or carbohydrate components of the leukocyte common antigen (lca), were used to characterize the expression pattern of different variants of this antigen complex. immunohistochemical and flow cytometric analysis was carried out on human lymphoid tissues from different anatomical compartments. we found that variations in terminal sialic acid or o-linked sugar moieties were associated with drastic differences in reactivity with some of t ... | 1991 | 1828514 |
| [serological demonstration of the detection of tick-borne rickettsial disease in astrakhan province]. | starting from 1978, noncontagious febrile diseases of unclear etiology, accompanied by pronounced headache, roseolous-papular eruptions, prolonged convalescence period, are registered in may-september in astrakhan province. these diseases can be effectively treated with chrolamphenicol. in 11 out of 12 sera obtained from such patients the complement fixation test with the antigens of rickettsiae causing tick-borne spotted fever, epidemic typhus, as well as coxiella burnetii antigen, revealed the ... | 1991 | 1831951 |
| history of veterinary public health in australasia. | the geographic isolation of australasia has played a significant role in preventing the introduction of exotic diseases or in limiting the spread of many diseases which entered after settlement. some infections such as psoroptic mange, tuberculosis and brucellosis became widely dispersed and some were ultimately to require novel methods to curtail them, e.g. greater use of rail and road transportation to convey stock, improved methods to locate and muster livestock in bush terrain (helicopters), ... | 1991 | 1840850 |
| epidemiology of q fever in sweden. | q fever is known to be a worldwide disease, with sweden supposed to be one of a few exceptions. the purpose of this pilot study was to elucidate whether or not a potential risk group for obtaining q fever in sweden was seropositive to the causative agent coxiella burnetii. blood samples were collected from sheep farmers on the island of gotland, and from members of their families. serum samples were examined by elisa for the presence of antibodies against c. burnetii, phases i and ii. positive r ... | 1991 | 1853162 |
| isolation of coxiella burnetii in sweden. | coxiella burnetii was isolated from sheep placentas, which had been collected from farms harbouring humans seropositive to the organism. the isolation of these bacteria is the final evidence that q fever is a domestic disease in sweden. | 1991 | 1853178 |
| mechanisms that may account for differential antibiotic susceptibilities among coxiella burnetii isolates. | the nine mile, s q217, and priscilla isolates, representative of the three major genetic groups of coxiella burnetii, are known to differ in their susceptibilities to antibiotics. mechanisms potentially responsible for these differences were investigated. accumulation of antibiotics by infected l929 cells and purified isolates was measured. in addition, c. burnetii plasmid-transformed escherichia coli hb101 cells were used to study the possibility that different c. burnetii plasmids are responsi ... | 1991 | 1854176 |
| use of monoclonal antibodies to identify cytochrome p450 isozymes in rat liver microsomes that hydroxylate n-nitrosomethylamylamine at each of six positions. | inhibition of enzyme activity by monoclonal antibodies (mabs) was used to indicate which cytochrome p450 isozymes in sprague-dawley rat liver microsomes catalyse hydroxylation of the oesophageal carcinogen n-nitrosomethyl-n-amylamine (nmaa) to give 2- to 5-hydroxy-nmaa (ho-nmaa), formaldehyde and pentaldehyde. liver microsomes (0.3-0.6 mg protein) were incubated (15 min, 23 degrees c) with 0.4 mg mab and, after adding nmaa to 6 mm, incubated for 20 min at 37 degrees c. mixtures were analysed for ... | 1991 | 1855887 |
| [the dynamics of the morphological changes in the tissues after the subcutaneous administration of vaccines against q fever]. | the comparative study of the dynamics of morphological changes in tissues of guinea pigs after the subcutaneous injection of chemical, live and combined vaccines against q fever during the period from 12 hours to 90 days was made. all vaccines under study were shown to produce a pronounced local damaging effect. two periods were tentatively discriminated in the dynamics of changes: the early phase (till 48 hours) and the late phase (days 2-90). at the early stage the most pronounced changes were ... | 1991 | 1858470 |
| [an immunoenzyme method for demonstrating coxiella burnetii antigens]. | materials on the development of an enzyme immunoassay (eia) system for the detection of the antigens of c. burnetii, the causative agent of q rickettsiosis, are presented. the system is highly specific and effective with respect to both corpuscular antigens of phases 1 and 2 and soluble antigen (lipopolysaccharide). the sensitivity of this method varies within the range 5-100 ng/ml. the effectiveness of eia as a quantitative (semiquantitative) control test used in the process of the production o ... | 1991 | 1867036 |
| incidence of q fever. | | 1991 | 1867607 |
| analysis of the cbhe' plasmid gene from acute disease-causing isolates of coxiella burnetii. | a gene termed cbhe' was cloned from the qph1 plasmid of coxiella burnetii. expression of recombinants containing cbhe' in vitro and in escherichia coli maxicells, produced an insert-encoded polypeptide of approx. 42 kda. the cbhe protein was not cleaved when intact maxicells were treated with trypsin. hybridizations of total dna isolated from the six strains of c. burnetii indicate that this gene is unique to c. burnetii strains associated with acute disease, i.e., hamilton[i], vacca[ii], and ra ... | 1991 | 1879692 |
| prolonged fever and pyuria: an uncommon manifestation of q fever. | a patient with q fever is described who had been ill for a year before the diagnosis was made on the basis of serological data. in addition it was possible to isolate coxiella burnetii, the causative agent by culture from the urine. this is very exceptional and is to our knowledge only the second case in which this has been achieved. the patient made a full recovery after lengthy treatment with tetracycline. q fever should be considered in patients with pyrexia of unknown origin, particularly in ... | 1991 | 1881505 |
| the seroprevalence of coxiellosis (q fever) in ontario sheep flocks. | a serological survey for coxiella burnetii was undertaken on a randomly selected population of 103 ontario sheep flocks. twenty-two flocks had at least one positive ewe; seven flocks had two or more reactors. the positive flocks were geographically clustered northwest of guelph. crutch-clipping of the ewe's wool prior to lambing, and total confinement housing at lambing in winter and spring seemed to lower the probability of seroreactivity of the flock (p less than 0.05). the study suggests that ... | 1991 | 1884293 |
| pathogenesis of rickettsial infections emphasis on q fever. | the underlying mechanisms at the organismic, cellular and molecular levels that account for rickettsial pathogenesis are beginning to be revealed. in the case of coxiella burnetii infection, relatively recent genetic and biochemical data, as well as drug susceptibility studies, indicate a correlation between isolate type and clinical disease--chronic or short-term acute. the use of cultured cells as model host systems has revealed that, indeed, different isolates from the major classified strain ... | 1991 | 1884773 |
| ecological questions concerning rickettsiae. | the past ten years were characterized by the appearance of several "new" transmissible spotted fever group (sfg) rickettsioses, e.g. israeli, japanese and astrakhan fevers. the factors responsible for their establishment probably include the introduction of chemicals from industry, agriculture and the timber industry into natural habitats. such factors may influence the pathogenicity of these rickettsiae. in this case, in addition to the human influence, the mechanism of the circulation of the a ... | 1991 | 1884774 |
| epidemiology of rickettsial diseases. | rickettsial diseases have a diversity of epidemiologic characteristics reflective of the variety of ecologic situations in which the obligate intracellular bacteria are transmitted to humans. for the spotted fever group (sfg) rickettsiae, rickettsia typhi, r. tsutsugamushi, coxiella burnetii, and the human ehrlichial agent, humans are a dead-end host who plays no role in the maintenance of the organism in nature. all rickettsioses exist as zoonoses. moreover, all rickettsiae are found in infecte ... | 1991 | 1884775 |
| antibiotic treatment of rickettsiosis, recent advances and current concepts. | the author reviews the recent advances in the treatment of mediterranean spotted fever and q fever. in mediterranean spotted fever (m.s.f.), in vitro and preliminary in vivo data support the place of quinolones and josamycin in the treatment of m.s.f. in children josamycin could become the first choice drug as well as in pregnant woman. in q fever chronic disease should be treated using a combination of antibiotic (doxycycline + quinolones) for a minimum of 3 years. | 1991 | 1884779 |
| control of rickettsial diseases. | prevention of rickettsial infections is aimed at individual control and epidemic measures (especially in epidemic typhus), vector and rodent control, milk pasteurization (in q fever), chemoprophylaxis and immunoprophylaxis. in vector and rodent control, the main obstacle is the rise in resistance to insecticides and rodenticides. for this reason in vector control, apart from insecticides, enhancement of the natural immunity acquired by animals in response to tick infestation and vaccination with ... | 1991 | 1884780 |
| rickettsiae and rickettsioses in portugal. | the only rickettsiae recorded in portugal till now were rickettsia conorii and coxiella burnetii. boutonneuse fever is one of the most important transmissible diseases in portugal. though the annual number of cases is not exactly known, it is estimated to be not far from 20,000 in some years. q fever is the other rickettsiosis widely disseminated throughout the country. the serological prevalence and the incidence of those rickettsioses in portugal are presented in this communication. in recent ... | 1991 | 1884782 |
| extensive examination of different tick species for infestation with coxiella burnetii in slovakia. | between 1987-1989 almost 7000 adult ixodes ricinus, dermacentor reticulatus, dermacentor marginatus, haemaphysalis concinna, haemaphysalis punctata and haemaphysalis inermis ticks collected in all 38 districts of slovakia were screened for the presence of coxiella burnettii. the proportion of ticks containing c. burnetii as indicated by the haemocyte test was less than 3%. attempts to recover c. burnetii by inoculation of yolk sacs of embryonated hen eggs from pools of 1-6 specimens of haemocyte ... | 1991 | 1884784 |
| epidemic of q fever in leszno district in poland. | in 1988 an epidemic of q fever was detected in leszno district. during 1973-1985 all 28,066 cattle tested for c. burnetii antibodies were found to be negative. the first seroconversions were found in cows which produced stillborn young. in the following years the number of seropositive cattle increased from 8.4% in 1987 to 21.6% in 1989. in 1988 all animal workers in the district were tested for c. burnetii antibodies. of 4,264 persons tested, 1,451 (34%) were seropositive. a detailed study of w ... | 1991 | 1884785 |
| horn fly (diptera: muscidae) insecticide resistance in kentucky and arkansas. | the effect of previous insecticide use patterns for horn fly control on the susceptibility spectrum of horn fly (haematobia irritans [l.]) populations from kentucky and arkansas is described. populations of horn flies from both states were tested with three pyrethroids (cyhalothrin, cypermethrin, and permethrin), three organophosphates (diazinon, pirimiphos methyl, and tetrachlorvinphos), and a chlorinated hydrocarbon (methoxychlor). dose-mortality data indicated insecticide resistance in arkans ... | 1991 | 1885841 |
| effects of environmental exposure on carbon polysulphone composites. | in vivo and in vitro studies were designed to characterize the material degradation associated with implantation of carbon fibre-reinforced polysulphone (c/ps). composite plates were compression moulded for both studies, and a fibre orientation of 0 degrees with the long axis and a fibre volume fraction of approximately 55% were used. the in vitro experiment involved soaking three groups of four plates each at 4, 12 and 26 wk in 0.9% saline solution at 37 degrees c. the plates were measured for ... | 1991 | 1888812 |
| the topographical and age distributions of neuroendocrine cells in the normal human appendix. | in order to clarify the histogenesis of appendiceal carcinoid tumours, epithelial (enc) and subepithelial (snc) neuroendocrine cells were counted at four sites in 50 normal appendices stained by standard argyrophil and argentaffin techniques. in general, enc were present in similar number at all sites within the appendix, whereas snc were more numerous at the tip than at the base. the number of enc was similar throughout life, apart from an increase in one neonate and some elderly patients, wher ... | 1991 | 1890548 |
| q fever in 1988-1989. | | 1991 | 1892743 |
| economic implications of bovine somatotropin use for the arizona dairy industry. | this study examined the impacts of bst on income of dairy producers in arizona. the nature of milk production in arizona, the institutional aspects of the milk order, and the supply management program of the united dairymen of arizona (the only milk cooperative in arizona) were considered in the model. prices declined with increased milk yields from bst. three dairy enterprise budgets demonstrated that gross revenue minus variable costs initially increased for adopters of bst but declined for no ... | 1991 | 1894823 |
| [epidemiology of community-acquired pneumonia in the health area i of navarra]. | during one year (1988), a protocol study of the community-acquired pneumonias was carried out in patients referred to the hospital virgen del camino in pamplona (health area i or northern navarra), so as to have an epidemiological and microbiological knowledge of this disease in this geographic area. | 1991 | 1895782 |
| [simultaneous infection by coxiella burnetii and chlamydia psittaci in 2 patients: the problem of psittacosis of non-avian origin]. | | 1991 | 1895793 |
| in vitro susceptibilities of coxiella burnetii, rickettsia rickettsii, and rickettsia conorii to the fluoroquinolone sparfloxacin. | in vitro susceptibilities of rickettsia rickettsii, rickettsia conorii, and coxiella burnetii to the new fluoroquinolone sparfloxacin (at-4140; rp 64206) were determined. plaque and dye uptake assays were used to measure the mics against r. rickettsii and r. conorii. the susceptibilities of c. burnetii nine mile and q 212 were determined in two acute-infection models and in two chronic-infection models. the mics were 0.125 to 0.25 microgram/ml for r. rickettsii and 0.25 to 0.5 microgram/ml for r ... | 1991 | 1901703 |
| localization of prostaglandin endoperoxide synthase in neurons and glia in monkey brain. | the localization of prostaglandin endoperoxide synthase in monkey brain was investigated by the immunoperoxidase method using the monoclonal antibody (pes-7) raised against the enzyme purified from bovine seminal vesicle. the frozen sections with 30-microns thickness were employed after the brain was fixed with perfusion of 2% paraformaldehyde in phosphate-buffered saline. the immunoreactivity was most intense in the neurons of cerebral cortex and hippocampus, and was moderate in the neurons of ... | 1991 | 1905180 |
| identity of hemolysins produced by bacillus thuringiensis and bacillus cereus. | a hemolysin (bt-hemolysin) produced by bacillus thuringiensis var. kurstaki hd-1 producing crystalline toxin(s) was purified by successive treatments of ammonium sulfate (45-65%) and column chromatography using deae-cellulose, sephadex g-75 and kb-002 (a hydroxyapatite column for fast protein liquid chromatography). a hemolysin (bc-hemolysin) produced by b. cereus hg-6a was also purified by the same procedure. the purified bt-hemolysin and bc-hemolysin, both of which are thiol-activated hemolysi ... | 1991 | 1905656 |
| role of serine biosynthesis and its utilization in the alternative pathway from glucose to glycogen during the response to insulin in cultured foetal-rat hepatocytes. | the role of serine as a possible intermediate of the alternative pathway from glucose to glycogen was investigated under basal and insulin-stimulated conditions in 18-day cultured foetal-rat hepatocytes because these cells cannot use pyruvate-derived metabolites [bismut & plas (1989) biochem. j. 263, 889-895]. incubation of cells with [u-14c]glucose for 24 h led to a release of labelled serine in the medium concomitantly with a net serine production (100 nmol/24 h per culture). the rate of [14c] ... | 1991 | 1905920 |