| banning drugs in sports: a skeptical view. | recent proposals to punish athletes for taking drugs or to impose mandatory drug testing cannot be defended in ethical terms. nor is it possible to distinguish consistently between ethical and unethical uses of restorative drugs, additive drugs, painkillers, and recreational drugs. we oppose drugs in sports because they violate the majority notion of acceptable behavior. but such opposition has more to do with defending the ideals of the community than with creating policies that are ethically s ... | 2013 | 3744803 |
| diverticulitis. | | 2014 | 4159011 |
| jaundice due to oxyphenisatin. | | 2014 | 4102455 |
| lithium prophylaxis. | | 2014 | 4097626 |
| blood and plasma ascorbic acid in indian childhood cirrhosis. | | 2016 | 4784731 |
| serum copper as an index of tumor response to radiotherapy. | | 2016 | 4734340 |
| coincidence of seizure susceptibility to caffeine and to the benzodiazepine inverse agonist, dmcm, in swr and cba inbred mice. | several lines of evidence suggest that the convulsant actions of caffeine are mediated through benzodiazepine receptors. a pharmacogenetic approach has been used to further explore the relationship of these receptors to caffeine-induced seizures. the susceptibility of two inbred strains of mice (cba and swr) to the convulsant actions of picrotoxinin, strychnine, ro 5-4864 and dmcm was examined. previous studies have demonstrated these two strains differ in their susceptibilities to the convulsan ... | 2013 | 3575358 |
| [experiences in team nursing in the leonberg district hospital]. | | 2015 | 4485309 |
| reflections from a decade of carotid reconstructive surgery. | carotid artery reconstructive surgery for atherosclerotic lesions of the extracranial cerebral circulation has become the most common operation in peripheral vascular surgery. a better understanding of the indications for operative intervention, enhanced monitoring during surgery, and more precise management of intraoperative anesthesia have all helped decrease the risks associated with internal carotid endarterectomy. to evaluate the safety and efficacy of extracranial carotid reconstructive su ... | 2012 | 3358162 |
| [current diagnosis and differential diagnosis of thyroid diseases]. | | 2002 | 119901 |
| [indications for relief and palliative operations]. | torkildsen and spitz-holter drainages and/or osteoclastic trepanation were performed on 29 patients suffering from a severe increase in intracranial pressure and/or a severely worsened general condition as a relieving intervention in order to permit a tumor operation later. the relieving measure enabled a tumor operation in 28 patients. the same procedure was used as a palliative step for another group of 73 patients suffering from inoperable tumorous processes. evaluation in these cases showed ... | 1999 | 95657 |
| cardiac tamponade and peripheral eosinophilia in a patient receiving cromolyn sodium. | | 2000 | 95924 |
| journal of autism and childhood schizophrenia. change of journal scope and title. | | 2000 | 86533 |
| the clinical response of 328 private patients to acupuncture therapy. | it is the attempt of this study to determine the efficacy of response to acupuncture at 24 hours, one week, and three week intervals at the end of a series of treatments in 328 patients. treated for a variety of disorders, approximately 60% had satisfactory responses of up to 3 weeks duration. the 328 patients were classified into 13 categories of problem disorders: headaches, cervical pain, lumbar pain without radiation, lumbar pain with radiation, isolated sciatica, parathoracic pain, knee pai ... | 2002 | 123703 |
| [thrombocytopenias. radioisotope studies. platelet survival studies]. | | 2002 | 120271 |
| mallet fractures. | contrary to our observations, reports in the literature maintain that only a third of mallet finger deformities are caused by intra-articular fractures of the posterior margin of the distal phalanx. as functional impairment sometimes remains after treatment, there is still much discussion as to the relative merits of conservative and surgical management of this lesion. this article presents 21 cases of mallet finger deformities with fracture of the distal phalanx, of which 17 received surgical t ... | 2012 | 3426323 |
| assessment of a bubble tonometer as a quality control system for blood gas analyzers (po2 and pco2). | a blood gas tonometer device, comprising an accurate gas mixer (corning 192) associated with a dual channel bubble tonometer (corning 184) was tested for reliability, accuracy and ease of use as a quality control system for po2 and pco2 blood gas analyzers. from our results it is apparent that this system can be used instead of older and more bulky tonometry devices, gas mixing systems or cylinders of fixed composition. | 2013 | 3889784 |
| histogenesis of malignant melanoma with an adjacent component of the superficial spreading type. | there has been a world-wide exponential increase in the incidence of thin malignant melanoma. at the sydney melanoma unit, the proportion of patients diagnosed as having superficial spreading melanoma has more than doubled from 33% prior to 1960 to 78% during 1980-83. a study was made of the non-invasive component of malignant melanoma with an adjacent non-invasive component of the superficial spreading type in an attempt to elucidate the pathogenetic mechanisms involved in these changing trends ... | 2014 | 4047726 |
| smoking habits of 611 patients with oral lichen planus. | the smoking habits of 611 patients (68 per cent females and 32 per cent males) with oral lichen planus were studied. forty-six per cent were daily smokers, 4 per cent smoked only at social events, and 50 per cent were nonsmokers. in comparison with the nonsmokers, the daily smokers showed significantly lower prevalences of reticular and atrophic types of oral lichen planus lesions and a significantly higher prevelance of the plaque type (p less than 0.001). it is suggested that these findings de ... | 1992 | 265045 |
| anesthesia for trauma. | anesthesia of polytraumatized patients represents a considerable risk for them. the proper treatment of a polytraumatized patient comprises the rapid stabilisation of all his vital functions. at the same time, the attempt must be made to achieve a complete picture of all his injuries in order to be able to select a reliable and safe anesthetic procedure. over and beyond this, a continuation of all intensive care measures, in particular the optimalization of blood volume, the maintenance of gas e ... | 1997 | 3327336 |
| advances in epithelial kinetics--an oral view. | there is growing evidence that the basal layer of stratified squamous epithelia consists of cells of differing proliferative capacities and behaviour. these can be classified as stem cells, transient amplifying cells and post-mitotic maturing cells, the most important of which appear to be the stem cells which may be present throughout life and are responsible for all cell production under steady-state conditions. recent stem cell concepts are reviewed for epidermis, intestinal crypt and oral mu ... | 1998 | 108372 |
| serum alphafetoprotein (afp) in bilharziasis. | two hundred and eighty-four patients of various ages from an endemic area, diagnosed as having bilharziasis by the presence of living schistosoma haematobium's ova in the mid-stream urine sample, have been investigated for the presence of alphafetoprotein (afp) in the serum. one hundred and forty-one (49.6%), were positive; no false positives have occurred. radioimmunoassay is capable of increasing the positivity rate still further. the detection of afp will be of limited value as a screening te ... | 2000 | 89881 |
| massive bleeding from a ruptured superior mesenteric artery aneurysm duodenum. | a traumatic false aneurysm of the superior mesenteric artery resulting from a gunshot wound five years previously, caused massive hemorrhage into the second portion of the duodenum. dissection, resection, and end-to-end anastomosis was successful in controlling bleeding into the gastrointestinal tract, but immediate postoperative angiography showed a reappearance of the aneurysm, and three months later, it bled into the retroperitoneum. the patient underwent further resection and two subsequent ... | 2002 | 130851 |
| [post-cesarean maternal mortality (analysis of 700 cases)]. | | 2002 | 1123162 |
| ocular findings in a form of retinitis pigmentosa with a rhodopsin gene defect. | ocular findings are presented in 17 unrelated patients with a form of autosomal dominant retinitis pigmentosa and the same c to a transversion in codon 23 of the rhodopsin gene. these patients (mean age, 36.6 years) had, on average, significantly better visual acuity and larger erg amplitudes than 131 unrelated patients (mean age, 32.1 years) with autosomal dominant retinitis pigmentosa without this mutation. these 17 patients from separate families as well as 11 relatives with the mutation from ... | 2005 | 2095030 |
| [precious metal onlays]. | in prosthetics, various options are available to the orthodontist for repairing dental arches. the basic choice is between full crowns and partial crowns. full crowns are primarily indicated for aesthetic reasons, in order to correct tooth axes and in cases of recurrent caries. partial crowns in precious alloys are indicated when the need is not essentially aesthetic. they continue today to offer advantages in restoring posterior teeth. because of their supragingival limits, they alleviate perio ... | 2007 | 2223666 |
| [precious metal onlays]. | in prosthetics, various options are available to the orthodontist for repairing dental arches. the basic choice is between full crowns and partial crowns. full crowns are primarily indicated for aesthetic reasons, in order to correct tooth axes and in cases of recurrent caries. partial crowns in precious alloys are indicated when the need is not essentially aesthetic. they continue today to offer advantages in restoring posterior teeth. because of their supragingival limits, they alleviate perio ... | 2007 | 2223666 |
| pneumothorax in the newborn with respiratory difficulties. | pneumothorax develops when extraalveolar air ruptures into the pleural space with partial or complete collapse of the affected lung. pneumothorax can occur at any time during the newborn period, but it usually appears as a complication of some other respiratory problem. the purpose of this study is to known the incidence of pneumothorax due to the respiratory difficulties. the materials were taken from 2368 neonates suffering from respiratory difficulties, out of 7445 neonates born in cipto mang ... | 2007 | 2075021 |
| enhanced cytotoxicity in simultaneous and sequential drug-heat treatments of cultured chinese hamster v79 cells. | under hyperthermal conditions, some genotoxic drugs such as bleomycin, paraquat, and n-alkyl-n-nitrosoureas exhibit increased cytotoxicity toward cultured chinese hamster v79 cells. sequential combinations of heat and drug treatments, regardless of whether drug-exposure precedes or follows hyperthermia, also induce synergistic cytotoxicity to some extent. this may be attributed not only to the relationship of temperature and chemical injury as defined by the arrhenius law, but also to a lethal i ... | 2008 | 2632055 |
| cell-free synthesis of deoxyhypusine. separation of protein substrate and enzyme and identification of 1,3-diaminopropane as a product of spermidine cleavage. | the post-translational formation of hypusine (n epsilon-(4-amino-2-hydroxybutyl)lysine) occurs in a precursor of eukaryotic initiation factor 4d by way of two major steps: 1) transfer of the 4-aminobutyl moiety from spermidine to the epsilon-amino group of a specific lysine residue to form an intermediate, deoxyhypusine; 2) hydroxylation of the deoxyhypusine residue to form hypusine. the initial step of this modification, deoxyhypusine synthesis, was studied in fractionated lysates of chinese ha ... | 2011 | 3139668 |
| re: prevention of low birthweight. | | 2011 | 3769854 |
| [prevention of tubal sterility]. | the prevention of tubal sterility necessitates not only the diagnosis and treatment of venereal infections, but also the prevention of post-partum, post-abortum, and especially iatrogenic infections. equally, the surgeon has a role to play, for many tubal sterilities follow traumatic surgical interventions in young women and adolescents. | 2013 | 3841407 |
| [histochemical detection of glycogen and rna in inflammatory hyperplasia of the palatal mucosa]. | | 2015 | 4516964 |
| [application of ethyl silicate amine method to dental investments for higher temperature casting]. | | 2015 | 4337899 |
| response force as an indicant of conflict in double stimulation. | | 2015 | 4745460 |
| nasogastric tube perforation of the nasopharynx. | we have presented two unusual cases of nasogastric tube perforation of the posterior nasopharynx. complications included subcutaneous emphysema, pneumomediastinum, and pleural effusion. both patients recovered. recommendations to improve nasogastric tube safety include gentle placement using lubricant and an appropriately sized tube, confirmation of tube placement before any infusion, and awareness of patients at increased risk of mucosal injury. | 2014 | 4071148 |
| [experience with psychotropic drugs, autogenic training and psychologic dialogs with the physician in rheumatic diseases]. | | 1993 | 309242 |
| [transformation of the organophosphorus pesticides, antio and chlorophos, in the environment]. | | 2000 | 95990 |
| cancer mortality and fluoridation. | | 2000 | 87581 |
| planning for future actions. workshop on public policy considerations. | | 2003 | 1868281 |
| ige and the nephrotic syndrome. | | 2003 | 4104752 |
| rupture of the abdominal oesophagus: a review. | 'spontaneous' rupture of the oesophagus usually affects the lower third, less commonly the middle third and rarely the cervical oesophagus. rupture limited to the intra-abdominal oesophagus is rare. such a case is described here and previously reported cases are reviewed. vomiting-induced gastro-oesophageal injuries and their aetiology are discussed and the management of spontaneous rupture is reviewed. | 2004 | 497641 |
| gentamicin volume of distribution as a power function of body weight. | gentamicin volume of distribution (v) was related to body weight (w) in 183 obese, normal weight and infant patients as reported in five published studies. in contrast to the usually postulated simple proportionality (v = bw) a linear function was found (v = a + bw) with an intercept (a = 2.5 +/- 0.49) significantly different from zero (p = 0.0001) and a slope (b = 0.19 +/- 0.10) significantly different (p = 0.0001) from the mean factor of proportion (b = 0.35 +/- 0.19). as compared with the lin ... | 2008 | 2590606 |
| secondary cytoreductive surgery in epithelial ovarian cancer: nonresponders to first-line therapy. | thirty-three patients with progressive or stable epithelial ovarian cancer who underwent secondary tumor-reductive surgery at the university of texas m. d. anderson cancer center were retrospectively studied. all patients had primary cytoreductive surgery followed by chemotherapy. lack of response to chemotherapy was clinically documented preoperatively. the tumors of 55% of the patients were secondarily cytoreduced to a greatest diameter of less than 2 cm. sixty-six percent of the patients requ ... | 2008 | 2703158 |
| chinese origin of correspondence-paper with black margins as used during mourning. | in europe there is the custom of observing mourning at the loss of a relative by using, even in correspondence, paper with a black margin. this may be traceable to china where a document on such a paper has been offered as the original. | 2008 | 2633626 |
| long-term prognosis after neonatal tetanus. | twenty-four turkish children who had had neonatal tetanus were evaluated by means of physical and neurological examinations and psychometric tests at four to 15 years of age. enuresis, mental retardation and growth retardation were frequent findings. the prevention of neonatal tetanus is important not only because of the high mortality rate but also because of its sequelae. | 2010 | 2920874 |
| detection and prevention of uterine cancer. | the two major uterine cancers, squamous carcinoma of the cervix and adenocarcinoma of the endometrium, are highly vulnerable to detection technology and, therefore, secondary prevention. high risk groups for each have been defined, cancer precursors described, i.e., dysplasia of the cervix and adenomatous hyperplasia of the endometrium, and efficient screening methods made available. for the cervical tumor cytologic testing is most appropriate, while histologic screening is more efficient for en ... | 2011 | 3167793 |
| elastosis perforans serpiginosa and pseudoxanthoma elasticum-like skin change due to d-penicillamine. | | 2011 | 4042411 |
| [diagnostic reliability of 201tl myocardial emission computed tomography and frank vectorcardiography for the evaluation of myocardial infarct sites]. | | 2012 | 3493376 |
| [soldering and welding]. | | 2012 | 4405775 |
| morphometric studies of the renomedullary interstitial cells. | the renomedullary interstitial cell (ric) is a unique cell found in the renal medullary interstitium among the collecting ducts, henle's loop, and the vasa recta. a combination of morphologic and physiologic studies has defined some of the properties of this cell, which is the apparent source of the mediators of the antihypertensive function of the kidney. these may include prostaglandins (pge2), aprl or platelet activating factor, and the incompletely defined neutral lipid, anrl (or medullipin) ... | 2012 | 3284348 |
| fluid retention and burn survival. | the relationship between retained fluid and survival has previously been reported from our burn unit. two hundred thirty cc/kg lean body mass accurately delineated survivors from nonsurvivors. our previous study did not account for fluid lost through the burn wound. this study was undertaken to evaluate fluid retention per square meter of body surface area which has been corrected for fluid loss from the burn wound using a formula suggested by mason et al. data collected from 95 adult patients w ... | 2013 | 3820349 |
| reminiscence--poor law pride. | | 2013 | 3910550 |
| [geriatrics and reciprocal expectations]. | | 2013 | 3764237 |
| failure of hcg treatment in depleting testicular cholesterol content of the musk shrew (suncus murinus l) in different seasons. | | 2013 | 3583336 |
| [carcinomatous neuromyopathy]. | | 2014 | 4290971 |
| [carcinomatous neuromyopathy]. | | 2014 | 4290971 |
| the effect of synovial fluid proteins in the degradation of hyaluronic acid induced by ascorbic acid. | the degradation of hyaluronic acid induced by ascorbic acid and the effect of synovial fluid proteins, such as ceruloplasmin, transferrin, and albumin, were investigated on the basis of the elution volume and the molecular weight of hyaluronic acid using high-performance gel permeation chromatography. hyaluronic acid was degraded to less than one-third of the original molecular weight in the range of the physiological concentrations of ascorbic acid. synovial fluid proteins protected against the ... | 2014 | 4009165 |
| [method of determining triethylenediamine (1,4-diazabicyclo-/2,2,2-octane) in sanitary-chemical studies of polymer materials]. | | 2014 | 4085843 |
| whistle-blowers take risk to halt wrongdoing. | | 2014 | 3893204 |
| [calcification of the cataract in childhood]. | | 2015 | 4684629 |
| the fine structure of the lutein cells in the mink (mustela vison) with special reference to the secretory activity during pregnancy. | | 2015 | 4352286 |
| [community mental health services and home visits. i]. | | 2015 | 4347366 |
| intercellular space conductance in frog gastric mucosa. | | 2016 | 5070077 |
| the mythology of electroconvulsive therapy. | | 2016 | 5063350 |
| [instrumental termination of labor (preliminaries on the use of the salinas forceps)]. | | 2016 | 5043626 |
| electromyographic studies of tension. | | 2016 | 4939791 |
| an overview of the conference on low level lead toxicity. | | 2016 | 4831143 |
| [urinary excretion of hydroxyproline in sarcoidosis]. | | 2016 | 4759228 |
| pathogenomic inference of virulence-associated genes in leptospira interrogans. | leptospirosis is a globally important, neglected zoonotic infection caused by spirochetes of the genus leptospira. since genetic transformation remains technically limited for pathogenic leptospira, a systems biology pathogenomic approach was used to infer leptospiral virulence genes by whole genome comparison of culture-attenuated leptospira interrogans serovar lai with its virulent, isogenic parent. among the 11 pathogen-specific protein-coding genes in which non-synonymous mutations were foun ... | 2013 | 24098822 |
| rapid and sensitive detection of bartonella bacilliformis in experimentally infected sand flies by loop-mediated isothermal amplification (lamp) of the pap31 gene. | carrion' disease, caused by bartonella bacilliformis, remains truly neglected due to its focal geographical nature. a wide spectrum of clinical manifestations, including asymptomatic bacteremia, and lack of a sensitive diagnostic test can potentially lead to a spread of the disease into non-endemic regions where competent sand fly vectors may be present. a reliable test capable of detecting b. bacilliformis is urgently needed. our objective is to develop a loop-mediated isothermal amplification ... | 2014 | 25522230 |
| succinyl-coa synthetase: new antigen candidate of bartonella bacilliformis. | bartonella bacilliformis is the causative agent of carrion's disease, a neglected illness with mortality rates of 40-85% in the absence of treatment. the lack of a diagnostic technique to overcome misdiagnosis and treat asymptomatic carriers is of note. this study aimed to identify new b. bacilliformis antigenic candidates that could lead to a new diagnostic tool able to be implemented in endemic rural areas. | 2016 | 27627803 |
| diagnosis of carrion's disease by direct blood pcr in thin blood smear negative samples. | bartonella bacilliformis is the etiologic agent of carrion's disease. this disease has two well established phases, the most relevant being the so called oroya fever, in which b. bacilliformis infect the erythrocytes resulting in severe anemia and transient immunosuppression, with a high lethality in the absence of adequate antibiotic treatment. the presence of b. bacilliformis was studied in 113 blood samples suspected of carrion's disease based on clinical criteria, despite the absence of a po ... | 2014 | 24651298 |
| proteins of bartonella bacilliformis: candidates for vaccine development. | bartonella bacilliformis is the etiologic agent of carrión's disease or oroya fever. b. bacilliformis infection represents an interesting model of human host specificity. the notable differences in clinical presentations of carrión's disease suggest complex adaptations by the bacterium to the human host, with the overall objectives of persistence, maintenance of a reservoir state for vectorial transmission, and immune evasion. these events include a multitude of biochemical and genetic mechanism ... | 2015 | 26413097 |
| oroya fever and verruga peruana: bartonelloses unique to south america. | bartonella bacilliformis is the bacterial agent of carrión's disease and is presumed to be transmitted between humans by phlebotomine sand flies. carrión's disease is endemic to high-altitude valleys of the south american andes, and the first reported outbreak (1871) resulted in over 4,000 casualties. since then, numerous outbreaks have been documented in endemic regions, and over the last two decades, outbreaks have occurred at atypical elevations, strongly suggesting that the area of endemicit ... | 2014 | 25032975 |
| carrion's disease: an eradicable illness? | carrion's disease is a neglected tropical disease caused by bartonella bacilliformis, a vector-borne pathogen restricted to the andean valleys of peru, ecuador and colombia. carrion's disease is a biphasic illness; in the acute phase the case-fatality rate can be as high as 88 %, related to high parasitemia, arriving to almost all erythrocytes, and secondary bacterial infections close related with the development of transient immunosuppression in the earlier illness phases. in addition, there ar ... | 2016 | 27903286 |
| whole-genome sequencing of two bartonella bacilliformis strains. | bartonella bacilliformis is the causative agent of carrion's disease, a highly endemic human bartonellosis in peru. we performed a whole-genome assembly of two b. bacilliformis strains isolated from the blood of infected patients in the acute phase of carrion's disease from the cusco and piura regions in peru. | 2016 | 27389274 |
| an outbreak of bartonella bacilliformis in an endemic andean community. | bartonellosis affects small andean communities in peru, colombia and ecuador. research in this area has been limited. | 2016 | 26991495 |
| evaluation of pcr approaches for detection of bartonella bacilliformis in blood samples. | the lack of an effective diagnostic tool for carrion's disease leads to misdiagnosis, wrong treatments and perpetuation of asymptomatic carriers living in endemic areas. conventional pcr approaches have been reported as a diagnostic technique. however, the detection limit of these techniques is not clear as well as if its usefulness in low bacteriemia cases. the aim of this study was to evaluate the detection limit of 3 pcr approaches. | 2016 | 26959642 |
| colonization of lutzomyia verrucarum and lutzomyia longipalpis sand flies (diptera: psychodidae) by bartonella bacilliformis, the etiologic agent of carrión's disease. | bartonella bacilliformis is a pathogenic bacterium transmitted to humans presumably by bites of phlebotomine sand flies, infection with which results in a bi-phasic syndrome termed carrión's disease. after constructing a low-passage gfp-labeled strain of b. bacilliformis, we artificially infected lutzomyia verrucarum and l. longipalpis populations, and subsequently monitored colonization of sand flies by fluorescence microscopy. initially, colonization of the two fly species was indistinguishabl ... | 2015 | 26436553 |
| an evaluation study of enzyme-linked immunosorbent assay (elisa) using recombinant protein pap31 for detection of antibody against bartonella bacilliformis infection among the peruvian population. | reliable laboratory testing is of great importance to detect bartonella bacilliformis infection. we evaluated the sensitivity and specificity of the enzyme-linked immunosorbent assay (elisa) using recombinant protein pap31 (rpap31) for the detection of antibodies against b. bacilliformis as compared with immunofluorescent assay (ifa). of the 302 sera collected between 1997 and 2000 among an at-risk peruvian population, 103 and 34 samples tested positive for ifa-immunoglobulin g (igg) and ifa-igm ... | 2014 | 24515944 |
| dried blood spots for qpcr diagnosis of acute bartonella bacilliformis infection. | bartonella bacilliformis is the etiological agent of a life-threatening illness. thin blood smear is the most common diagnostic method for acute infection in endemic areas of peru but remains of limited value because of low sensitivity. the aim of this study was to adapt a b. bacilliformis-specific real-time polymerase chain reaction (pcr) assay for use with dried blood spots (dbs) as a sampling method and assess its performance and use for the diagnosis and surveillance of acute bartonella infe ... | 2013 | 24043691 |
| bartonella bacilliformis: a systematic review of the literature to guide the research agenda for elimination. | carrion's disease affects small andean communities in peru, colombia and ecuador and is characterized by two distinct disease manifestations: an abrupt acute bacteraemic illness (oroya fever) and an indolent cutaneous eruptive condition (verruga peruana). case fatality rates of untreated acute disease can exceed 80% during outbreaks. despite being an ancient disease that has affected populations since pre-inca times, research in this area has been limited and diagnostic and treatment guidelines ... | 2012 | 23145188 |
| whole-genome analysis of bartonella ancashensis, a novel pathogen causing verruga peruana, rural ancash region, peru. | the genus bartonella contains >40 species, and an increasing number of these bartonella species are being implicated in human disease. one such pathogen is bartonella ancashensis, which was isolated in blood samples from 2 patients living in caraz, peru, during a clinical trial of treatment for bartonellosis. three b. ancashensis strains were analyzed by using whole-genome restriction mapping and high-throughput pyrosequencing. genome-wide comparative analysis of bartonella species showed that b ... | 2017 | 28221130 |
| development and characterisation of highly antibiotic resistant bartonella bacilliformis mutants. | the objective was to develop and characterise in vitro bartonella bacilliformis antibiotic resistant mutants. three b. bacilliformis strains were plated 35 or 40 times with azithromycin, chloramphenicol, ciprofloxacin or rifampicin discs. resistance-stability was assessed performing 5 serial passages without antibiotic pressure. mics were determined with/without phe-arg-β-napthylamide and artesunate. target alterations were screened in the 23s rrna, rpld, rplv, gyra, gyrb, parc, pare and rpob ge ... | 2016 | 27667026 |
| mutation-driven divergence and convergence indicate adaptive evolution of the intracellular human-restricted pathogen, bartonella bacilliformis. | among all species of bartonella, human-restricted bartonella bacilliformis is the most virulent but harbors one of the most reduced genomes. carrión's disease, the infection caused by b. bacilliformis, has been afflicting poor rural populations for centuries in the high-altitude valleys of the south american andes, where the pathogen's distribution is probably restricted by its sand fly vector's range. importantly, carrión's disease satisfies the criteria set by the world health organization for ... | 2016 | 27167125 |
| highly sensitive loop-mediated isothermal amplification for the detection of leptospira. | leptospirosis is a worldwide zoonosis caused by an infection with the pathogenic species of leptospira. we have developed a loop-mediated isothermal amplification (lamp) assay to detect the dna of leptospira spp. six sets of primers targeting the gene of the subsurface protein, lipl32, were evaluated for their detection sensitivity. the best primer set detected less than 25 copies of lipl32 per reaction of both plasmid dna template and purified leptospiral genomic dna. by combining primers targe ... | 2015 | 26904743 |
| multi-locus sequence typing of bartonella bacilliformis dna performed directly from blood of patients with oroya's fever during a peruvian outbreak. | bartonella bacilliformis is the etiological agent of carrion's disease, a neglected tropical poverty-linked illness. this infection is endemic of andean regions and it is estimated that approximately 1.7 million of south americans are at risk. this bacterium is a fastidious slow growing microorganism, which is difficult and cumbersome to isolate from clinical sources, thereby hindering the availability of phylogenetic relationship of clinical samples. the aim of this study was to perform multi l ... | 2016 | 26824740 |
| [antimicrobial resistance of bartonella bacilliformis strains from regions endemic to bartonellosis in peru]. | to evaluate in vitro antimicrobial susceptibility to chloramphenicol (chl) and ciprofloxacin (cip) in strains of bartonella bacilliformis from areas that are endemic to bartonellosis in peru, through three laboratory methods. | 2015 | 26732912 |
| complete genome sequence of bartonella ancashensis strain 20.00, isolated from the blood of a patient with verruga peruana. | here we present the complete genome sequence of bartonella ancashensis strain 20.00, isolated from the blood of a peruvian patient with verruga peruana, known as carrion's disease. bartonella ancashensis is a gram-negative bacillus, phylogenetically most similar to bartonella bacilliformis, the causative agent of oroya fever and verruga peruana. | 2015 | 26543106 |
| paleomicrobiology of bartonella infections. | studying ancient infectious diseases is a challenge, as written contemporary descriptions, when available, are often imprecise and do not allow for accurate discrimination among the pathogens endemic at that time. paleomicrobiology offers a unique access to the history of these infections by identifying precisely the causative agents. body louse-transmitted infections are amongst the most epidemic diseases in history, especially in war and famine periods. of these, bartonella quintana was detect ... | 2016 | 26369716 |
| antibiotic resistance in bartonella bacilliformis clinical isolates from an endemic area of peru. | | 2015 | 27873715 |
| description of bartonella ancashensis sp. nov., isolated from the blood of two patients with verruga peruana. | three novel isolates of the genus bartonella were recovered from the blood of two patients enrolled in a clinical trial for the treatment of chronic stage bartonella bacilliformis infection (verruga peruana) in caraz, ancash, peru. the isolates were initially characterized by sequencing a fragment of the glta gene, and found to be disparate from b. bacilliformis. the isolates were further characterized using phenotypic and genotypic methods, and found to be genetically identical to each other fo ... | 2015 | 26296673 |
| infection-related hemolysis and susceptibility to gram-negative bacterial co-infection. | increased susceptibility to co-infection with enteric gram-negative bacteria, particularly non-typhoidal salmonella, is reported in malaria and oroya fever (bartonella bacilliformis infection), and can lead to increased mortality. accumulating epidemiological evidence indicates a causal association with risk of bacterial co-infection, rather than just co-incidence of common risk factors. both malaria and oroya fever are characterized by hemolysis, and observations in humans and animal models sug ... | 2015 | 26175727 |
| an unidentified cluster of infection in the peruvian amazon region. | bartonella bacilliformis is the etiological agent of carrion's disease, which is a neglected disease linked to people in low-socioeconomic populations in andean valleys. an outbreak of b. bacilliformis was reported in a rural area of the peruvian amazon region. the aim of this study was to characterize this outbreak using molecular techniques. | 2015 | 25989173 |
| possible vertical transmission of bartonella bacilliformis in peru. | a 22-day-old male was admitted with a 2-day history of irritability, dyspnea, jaundice, fever, and gastrointestinal bleeding. a thin blood smear was performed, which showed the presence of intraerythrocyte bacteria identified as bartonella bacilliformis, and subsequently, the child was diagnosed with carrion's disease. the diagnosis was confirmed by specific polymerase chain reaction. the child was born in a non-endemic b. bacilliformis area and had not traveled to such an area before hospitaliz ... | 2015 | 25371184 |
| [carrión's disease complicated with severe acute leptospirosis: a case report]. | the case of an adult, 56 year old male is reported, from sihuas (ancash) who presented at hospital nacional dos de mayo with fever, jaundice and anemia. in three days of hospitalization, he developed severe anemia (hb: 11.8 to 6.7 g / dl) and generalized myalgias associated with a sudden worsening jaundice correlated with elevated bilirubin (tb 3.2 to 19.6 mg / dl direct dominance) and general deterioration. microbiological smear and culture were positive for bartonella bacilliformis. subsequent ... | 2014 | 25123883 |
| bartonellosis: one health perspectives for an emerging infectious disease. | in recent years, an increasing number of bartonella species have been identified as zoonotic pathogens, transmitted by animal bites, scratches, arthropods and even by needle sticks. considering the diversity of newly discovered bartonella species and subspecies and the large number and ecologically diverse animal reservoir hosts and the evolving spectrum of arthropod vectors that can transmit these bacteria among animals and humans, the clinical and diagnostic challenges posed by bartonella tran ... | 2014 | 24936029 |
| pathogenicity and treatment of bartonella infections. | bartonella spp. are responsible for emerging and re-emerging diseases around the world. the majority of human infections are caused by bartonella henselae, bartonella quintana and bartonella bacilliformis, although other bartonella spp. have also been associated with clinical manifestations in humans. the severity of bartonella infection correlates with the patient's immune status. clinical manifestations can range from benign and self-limited to severe and life-threatening disease. clinical con ... | 2014 | 24933445 |
| frequencies of blood group systems mns, diego, and duffy and clinical phases of carrion's disease in amazonas, peru. | carrion's disease (cd), is a human bartonellosis, that is, endemic in the andes of peru, ecuador, and colombia. bartonella bacilliformis, a native hemotrophic bacteria, is the causative agent of cd, and the interaction with the host could have produced changes in the gene frequencies of erythrocyte antigens. the goal here is to investigate the relationship between allele frequencies of blood group systems mns, diego, and duffy and the clinical phases of cd, within a genetic context. in this asso ... | 2014 | 24847360 |