| normal and abnormal metabolism in injured rat brains by p-31 nmr spectroscopy. | | 1989 | 2519845 |
| a method for bronchoalveolar lavage in live pigs. | in order to isolate porcine alveolar macrophages and to quantitatively study the components of recovered lung fluid, a bronchoalveolar lavage technique in living pigs was developed. lung lavage was performed after introducing a catheter through the mouth via the trachea in the diaphragmatical lobe. thirty ml of phosphate-buffered saline (pbs) was introduced into the lung and the fluid was aspirated after one minute. following this, another 15 ml of pbs was introduced into the lung and aspirated ... | 1989 | 2525834 |
| toxicity of haemophilus pleuropneumoniae to porcine lung macrophages. | viable haemophilus pleuropneumoniae bacteria were toxic for porcine alveolar macrophages in vitro. this cytotoxic effect proved to be dose-related. a cell-free extract of h. pleuropneumoniae, heat-killed bacteria, and a pasteurella multocida field strain were nontoxic. when macrophages were cultured with h. pleuropneumoniae bacteria in a ratio of 100 macrophages to six bacteria, ultrastructural signs of cellular degeneration were observed within 1 h. this degeneration was observed in macrophages ... | 1989 | 2526407 |
| serotype-related differences in production and type of heat-labile hemolysin and heat-labile cytotoxin of actinobacillus (haemophilus) pleuropneumoniae. | reference strains of serotypes 1 to 12 of actinobacillus (haemophilus) pleuropneumoniae were cultured in eagle minimal essential medium with 10% serum plus. culture supernatants were examined for cytotoxicity to alveolar macrophages and for the ability to hemolyze sheep erythrocytes. all strains except the reference strain of serotype 6 produced cytotoxin, whereas only serotypes 1, 5, 9, 10, and 11 produced hemolysin. both cytotoxin and hemolysin appeared to be heat labile. antisera were raised ... | 1989 | 2526820 |
| [the influence of previous infections on the development of experimental swine pleuropneumonia in specific pathogen free pigs]. | this study was designed to determine in six-week old specific pathogen free pigs, the effect of previous experimental exposure to mycoplasma hyopneumoniae and transmissible gastroenteritis virus on a challenge infection with actinobacillus pleuropneumoniae. pigs exposed simultaneously to m. hyopneumoniae and transmissible gastroenteritis virus appeared more resistant to challenge (one week later) with a. pleuropneumoniae. four pigs out of a group of ten died following the challenge infection, co ... | 1989 | 2531628 |
| comparison of conventional and long-acting oxytetracyclines in prevention of induced actinobacillus (haemophilus) pleuropneumoniae infection of growing swine. | these experiments tested the hypothesis that long-acting oxytetracycline (oxytetracycline-la) was more effective than regular oxytetracycline in preventing porcine pleuropneumonia when administered either 24 or 48 h prior to experimental challenge with virulent strains of actinobacillus pleuropneumoniae. two experiments (1 and 2) were conducted using growing pigs (average weight 12-15 kg). antibiotic treatments were administered once intramuscularly at 20 mg/kg body weight; controls received an ... | 1989 | 2531629 |
| responses of haemophilus pleuropneumoniae to iron restriction: changes in the outer membrane protein profile and the removal of iron from porcine transferrin. | outer membranes from haemophilus pleuropneumoniae grown under iron-replete and iron-restricted conditions in vitro were analysed by means of sds-page and immunoblotting. iron restriction resulted in the appearance of two or more novel polypeptides in the molecular size range of 96-102 kd and an increased amount of a 79 kd polypeptide. these polypeptides were recognized by porcine immune sera indicating their production by h. pleuropneumoniae during growth in vivo. although soluble siderophore pr ... | 1989 | 2532702 |
| endobronchial inoculation of various doses of haemophilus (actinobacillus) pleuropneumoniae in pigs. | twelve-week-old specific-pathogen-free pigs were inoculated deep in the bronchi with haemophilus (actinobacillus) pleuropneumoniae strain 13261 in doses ranging from 8 x 10(1) to 9 x 10(7) colony-forming units (cfu). pigs that survived infection were euthanatized and examined 48 hours after inoculation. the relationship between dose and severity of disease was evaluated clinically and the weight of pneumonic lesions was compared. the relationship between infection dose and weight of pneumonic le ... | 1989 | 2532869 |
| characterization of a streptomycin-sulfonamide resistance plasmid from actinobacillus pleuropneumoniae. | an actinobacillus pleuropneumoniae strain contained a plasmid (phd8.1) conferring resistance to streptomycin and sulfonamide. restriction endonuclease mapping and dna-dna hybridization showed that phd8.1 is related to rsf1010 from salmonella panama, which also confers resistance to streptomycin and sulfonamide, and to phd148 from haemophilus ducreyi, which confers resistance only to sulfonamide. | 1989 | 2541656 |
| development of a shuttle vector and a conjugative transfer system for actinobacillus pleuropneumoniae. | an original genetic system for actinobacillus pleuropneumoniae has been developed. a shuttle cloning vector, pyg53, was constructed from the wild-type plasmid pyg10. it permits, in conjunction with electroporation, the introduction of cloned genes into this species. a conjugal transfer system between escherichia coli and a. pleuropneumoniae involving pyg54, a mobilizable derivative of pyg53, is also described. conjugation efficiencies of 8.3 x 10(-3) exconjugants per donor can be obtained. | 1989 | 2559875 |
| [acute maternal anterior poliomyelitis in a non-endemic zone]. | the authors report the case of a 26 year old woman with acute anterior poliomyelitis contracted during the vaccination of her baby. despite having been herself vaccinated in infancy she was not protected against the poliovirus. the clinical interest of this uncommon case is a severe paralytic state with definitive paraplegia. the authors suggest serologic testing of patients born before 1967 especially if they are at risk of encountering the virus. | 1989 | 2561040 |
| effects of exercise, cold, and immobilization stresses on gamma-glutamyltransferase activity in rat kidney. | effects of acute and chronic stresses (exercise, cold, and immobilization) on gamma-glutamyltransferase (gamma gt) activity in the rat kidney were investigated. in the extramicrosomal fraction there were significant decreases in acute-exercised rats but significant increases in cold-adapted and immobilized rats. in the microsomal fraction, on the other hand, the gamma gt activity of acute-exercised rats increased definitely. the results suggest that different types of stresses have different eff ... | 1989 | 2576675 |
| nerve growth factor counteracts the neurophysiological and neurochemical effects of chronic sciatic nerve section. | the sciatic nerve was sectioned unilaterally in rats and nerve growth factor (ngf) applied locally to the nerve stump for the following 10-14 days using an indwelling osmotic pump. the aim of the experiment was to test whether ngf had any effect on the previously reported neurophysiological and neurochemical events that occur central to a peripheral nerve lesion. the method of application allowed the sciatic nerve on the other side to be used as a control. primary afferent depolarization fell, a ... | 1985 | 2581648 |
| expression of an hla-bw6-related specificity by the hla-cw3 molecule. | radioimmunoassay of hla-transformed mouse l cells expressing a3, a24, b7, or cw3 hla class i molecules with a set of monomorphic monoclonal antibodies distinguishes between a3-a24 and b7-cw3 patterns of reactivity. analyses with bw6-specific monoclonal antibodies and a human alloantiserum demonstrate the expression by the hla-cw3 molecules of a bw6 public specificity related to but not identical with that expressed by the hla-b7 molecules. exon-shuffling experiments and inhibition studies of mon ... | 1985 | 2581891 |
| radical formation in the rat small intestine during and following ischemia. | oxidative loading during the reperfusion of the proximal jejunum of rats following a one hour-period of complete ischemia was demonstrated in in vivo-experiments by the increases of the gssg: total glutathione ratio and the concentration of tba-rs. the pretreatment of the animals with the xanthine oxidoreductase inhibitor allopurinol diminished the accumulation of gssg and of tba-rs. it was concluded that the purine nucleotide degradation is an important source of oxygen reduction products in re ... | 1989 | 2583551 |
| attenuation of reserpine-induced catalepsy by melatonin and the role of the opioid system. | several reports have indicated that melatonin influences motor activity in animals and humans. melatonin has been reported to attenuate the rigidity and tremor of parkinson's disease. some of the behavioral effects (e.g., analgesic and anticonvulsant properties) of melatonin have been reported to be mediated through interactions with the endogenous opioid peptides. we investigated the effect of melatonin on reserpine-induced catalepsy in the rat and, additionally, examined whether this effect is ... | 1989 | 2583945 |
| isolation of actinobacillus (haemophilus) pleuropneumoniae from pneumonic lungs of slaughtered pigs. | | 1989 | 2601240 |
| [the synergism of actinobacillus pleuropneumoniae and influenza a virus in experimentally-infected mice]. | models for infecting mice with influenza a-virus (a/pr 8/34, h0n1) and actinobacillus pleuropneumoniae (serotype 9) were developed in han: nmri-mice. after infecting mice with sublethal doses of one of the infectious agents, or both together as a mixed infection, animals were subsequently exsanguinated and the lungs washed by bronchoalveolar lavage. clinical symptoms were recorded daily, examination of lung lavage fluid and sera as well as histology of the lungs were done. an increase in mortali ... | 1989 | 2609806 |
| autosomal dominant retinitis pigmentosa: exclusion of a gene from extensive regions of chromosomes 6, 13, 20, and 21. | members of a large pedigree of irish origin presenting with early onset type i autosomal dominant retinitis pigmentosa (adrp) have been typed for polymorphic dna markers from chromosomes 6, 13, 20, and 21. for each marker close linkage to adrp has been excluded by pairwise analyses. using distances fixed from well-established genetic maps of these chromosomes and multipoint analyses with two or three contiguous markers, exclusion of adrp was extended to the areas between markers, resulting in th ... | 1989 | 2613243 |
| primary structure of a ribonuclease from bullfrog (rana catesbeiana) liver. | a pyrimidine base-specific ribonuclease was purified from bullfrog (rana catesbeiana) liver by means of cm-cellulose column chromatography and affinity chromatography on heparin-sepharose cl-6b, which gave single band on sds-slab electrophoresis. the primary structure of the bullfrog liver rnase was determined. it consisted of 111 amino acid residues, including 8 half-cystine residues. from the sequence, it was concluded that three disulfide bridges in rnase a were conserved in the bullfrog rnas ... | 1989 | 2613682 |
| transformation of actinobacillus pleuropneumoniae and analysis of r factors by electroporation. | an efficient method for dna transfer is essential for the genetic manipulation of any organism. such a capacity will be required for the genetic analysis of actinobacillus pleuropneumoniae as a swine pathogen, as well as for its manipulation for vaccination purposes. for this reason, the use of electroporation as a means of plasmid dna introduction into this species was examined. the multiple antibiotic-resistant strain 80-8141 of actinobacillus pleuropneumoniae harbors 3 plasmids: pyg10, pyg15, ... | 1989 | 2619125 |
| malignant hyperpyrexia. | | 1989 | 2653537 |
| haemophilus pleuropneumoniae in pigs: a review. | haemophilus (actinobacillus) pleuropneumoniae is a primary and specific pathogen of the respiratory tract and is an economically important pathogen of pigs. the disease is starting to cause peracute deaths in south africa and the chronic form leads to deteriorating heard performance. this review highlights various aspects of the taxonomic, antigenic, and drug sensitivity characteristics of the bacterium and the epidemiology, clinical signs, pathology, serology and immunology, detection and diagn ... | 1989 | 2657061 |
| cloning and expression of a cohemolysin, the camp factor of actinobacillus pleuropneumoniae. | the genetic determinant of the cohemolysin which is responsible for the camp phenomenon, a cohemolysis, of actinobacillus pleuropneumoniae was cloned in escherichia coli. total dna from the a. pleuropneumoniae serotype 1 type strain 4074 was used to construct a gene library in plasmid puc18 in e. coli jm83. a total of 10,500 clones containing recombinant plasmids have been screened for hemolysis on blood plates. fifty-five clones which showed a weak hemolytic response after 24 to 48 h of incubat ... | 1989 | 2659534 |
| inhibition of proteoglycan synthesis alters extracellular matrix deposition, proliferation, and cytoskeletal organization of rat aortic smooth muscle cells in culture. | arterial proteoglycans have been implicated in several important physiological processes ranging from lipid metabolism to regulation of smooth muscle cell growth. vascular smooth muscle (vsm) cells are the major producers of proteoglycans in the medial layer of blood vessels. to study functional consequences of alterations in vsm proteoglycan metabolism we used 4-methylumbelliferyl-beta-d-xyloside to inhibit proteoglycan synthesis in primary and early passage cultures of rat aortic smooth muscle ... | 1989 | 2661564 |
| the effects of acute and chronic alcoholism on tumor necrosis factor and the inflammatory response. | ethanol intoxication has been shown to suppress selected functions of the immune system, thereby compromising host defenses against bacterial infections. because the macrophage secretory protein, tumor necrosis factor (tnf), plays a central role in the inflammatory cascade, the effect of acute and chronic alcoholism on lipopolysaccharide (lps)-induced tnf activity was studied. saline or ethanol was given intraperitoneally to normal or chronic alcoholic rats followed 30 min later by either intrav ... | 1989 | 2668425 |
| opsonization of yeast by the plasma of biomphalaria glabrata (gastropoda): a strain-specific, time-dependent process. | phagocytosis of yeast by haemocytes of biomphalaria glabrata, intermediate host of schistosoma mansoni, is influenced strongly by host plasma components, although it can occur without involvement of such factors. plasma from two strains of b. glabrata which are resistant to s. mansoni differs in its opsonic properties from the plasma of a susceptible strain. this may reflect the principles which determine compatibility or incompatibility in this host-parasite system. opsonization is a time-depen ... | 1989 | 2671868 |
| immunoserological comparison of 104-kilodalton proteins associated with hemolysis and cytolysis in actinobacillus pleuropneumoniae, actinobacillus suis, pasteurella haemolytica, and escherichia coli. | a homologous polyclonal antibody was produced in a rabbit to the 104-kilodalton (kda) protein hemolysin of actinobacillus pleuropneumoniae serotype 1 strain cm-5. in immunoblots, this antibody recognized a similar 104-kda protein produced in culture supernatants by a. pleuropneumoniae serotypes 1 to 12 and taxon "minor group" in addition to pasteurella haemolytica, actinobacillus suis, and alpha-hemolysin-producing escherichia coli (but only weakly in the latter two organisms). these results wer ... | 1989 | 2674017 |
| [hospital strains of salmonella serotype typhimurium with particular biological properties]. | 93 cultures of salmonella typhimurium (stm) were isolated from the infants (under one year old) admitted into a pediatrics ward. all the cultures had only flagellation antigens in phase i, a common sensitivity spectrum to antibiotics and the same sensitivity to the sets of bacteriophages for typing of this serotype. of these, 10 strains were negative h2s and 6 immobile. using as carrier a strain of e. coli, k12nxr, an experimental transfer of multiple resistance to the antibiotics obtained from ... | 1989 | 2675270 |
| cloning of a dna sequence encoding a major fragment of the 47 kilodalton stress protein homologue of candida albicans. | part of the gene coding for the immunodominant 47 kda antigen of the human fungal pathogen candida albicans was cloned and sequenced. the predicted amino acid sequence was homologous to stress proteins, the most extensive similarity being with the heat shock protein hsp 90 of saccharomyces cerevisiae. the 47 kda antigen has diagnostic and therapeutic potential and is the first candidal antigen to be cloned and sequenced. | 1989 | 2676706 |
| [therapeutic implication of plasma c3 rich fraction against bacterial infection in surgical patients]. | the depleted plasma opsonic activity is a major provoking cause of severe postoperative infection in patients who underwent extended surgical procedures. in order to establish the therapeutic use of c3 rich fraction (c3rf) for these patients in place of large amount of fresh frozen plasma (ffp) replacement, a basic study was done dealt with this fraction which was extracted from ffp with polyethyleneglycol. the results were as follows. (1) addition of minimum amount of c3rf in vitro sufficiently ... | 1989 | 2677664 |
| [biosynthesis of the membrane protein of the acquired immunodeficiency syndrome virus (hiv) with a deleted hydrophobic region in e. coli cells]. | effect of the structure of cloned env gene of hiv virus on the level of its expression has been studied. the deletion of the hydrophobic region from the env gene has been shown to increase considerably the biosynthesis of antigen-specific proteins in escherichia coli cells. at the same time, the low level of expression of the gene fragment the transcription of which is controlled by a powerful lac-promoter suggests the presence of toxic regions of nonhydrophobic nature in the synthesized antigen ... | 1989 | 2682218 |
| functional organization of the extrinsic and intrinsic circuitry of the parahippocampal region. | | 1989 | 2682783 |
| characterization of a multiple antibiotic resistance plasmid from haemophilus ducreyi. | plasmid pls88 from a clinical isolate of haemophilus ducreyi encoded resistance determinants for sulfonamides and streptomycin related to those of rsf1010 and for kanamycin related to tn903 but lacked the inverted repeats of the transposon. its host range included haemophilus influenzae, actinobacillus pleuropneumoniae, and escherichia coli; and it was compatible with pdm2 and rsf1010. | 1989 | 2684012 |
| mitochondrial behaviour during the yeast-hypha transition of candida albicans. | yeast cells of candida albicans were brought to germ tube formation and hyphal growth in liquid synthetic medium. the behaviour of mitochondria and mitochondrial nucleoids (mt-nucleoids) during morphological conversion was examined by fluorescence staining with 2-(4-dimethylaminostyryl)-1-methylpyridinium iodide (daspmi) and 4',6-diamidino-2-phenylindole (dapi). parent yeast cells possessed one or very few branched giant mitochondria which were stained intensely with daspmi. when a germ tube eme ... | 1989 | 2691864 |
| cloning and characterization of a hemolysin gene from actinobacillus (haemophilus) pleuropneumoniae. | neutralizing antisera to the leukotoxin secreted by pasteurella haemolytica neutralized the hemolysin of actinobacillus pleuropneumoniae and recognized a 110-kd antigen in cell-free culture supernatants from this organism. a series of nine overlapping recombinant phage clones carrying the gene for this 110-kd antigen were identified using affinity-purified anti-hemolysin antibody and a dna probe containing sequences from the p. haemolytica lktca genes. eight of the nine clones expressed a 110-kd ... | 1989 | 2693022 |
| an overview of australia's poultry industry in 1989. | | 1989 | 2695040 |
| risk of carcinogenesis from exposure to dietary mutagens. | epidemiological studies revealed the crucial role of dietary habits in human cancer. this review will summarize literature data on naturally occurring mutagens and carcinogens. the risk of carcinogenesis related to dietary mutagens will be discussed. | 1989 | 2698602 |
| in vitro evaluation of various quinolone antibacterial agents against veterinary mycoplasmas and porcine respiratory bacterial pathogens. | the in vitro activities of 12 quinolones and four antibiotics were determined against 15 veterinary mycoplasmal species and four species of bacteria commonly involved in respiratory infections in pigs. the newer quinolones were markedly more active in vitro against a wide range of mycoplasmas than nalidixic acid and the earlier quinolones. against mycoplasma hyopneumoniae ciprofloxacin was the most active quinolone with a geometric mean minimal inhibitory concentration (mic) against 16 strains o ... | 1989 | 2704885 |
| identification of the heat-labile hemolysin of actinobacillus pleuropneumoniae serotype 1. | the heat-labile hemolysin of actinobacillus pleuropneumoniae serotype 1 strain cm-5 was partially purified using ammonium sulfate precipitation and gel permeation chromatography. this partially purified material was subjected to sodium dodecyl sulfate-polyacrylamide gel electrophoresis and blotted onto nylon filters. the filters were treated with convalescent pig serum and subsequently with cm-5 culture supernatant containing active hemolysin. a 104 kd peptide was identified as the hemolysin bec ... | 1989 | 2713790 |
| nasal 'flow' receptors of the rat. | this study was performed to identify trigeminal nasal 'flow' receptors and to investigate their firing characteristics. for this purpose, single unit afferent activity was recorded from the anterior ethmoidal nerve in anesthetized rats breathing through the nose or a tracheostomy. in fourteen rats breathing through the nose, 40 of 73 endings tested were identified as 'flow' receptors for the following characteristics: their spontaneous activity had an inspiratory modulation that disappeared duri ... | 1989 | 2717813 |
| callosal projection neurons in area 17 of the fetal rhesus monkey. | we have studied the distribution of callosal projection neurons in area 17 of a fetal rhesus monkey which received large injections of horseradish peroxidase into the contralateral occipital cortex. in comparison to other cortical areas, area 17 contains few callosal projection neurons. most of these cells are confined to a region extending tangentially about 2.5 mm from the 17/18 border, although a few neurons were noted as much as 5 mm from the border. comparing the distribution of callosal pr ... | 1989 | 2720962 |
| [serologic studies on the presence of antibodies against haemophilus (actinobacillus) pleuropneumoniae at swine breeding farms in north brabant]. | serological screening for the presence of antibodies to both h. pleuropneumoniae serotype 2 and serotype 9 using the complement fixation test was carried out on twenty-nine piglets production farms of an integrated group in the province of north brabant. antibodies to h. pleuropneumoniae serotype 2 and serotype 9 were not detected on six of the twenty-nine farms when a single screening was performed. the proportions of farms with animals serologically positive in regard to serotypes 2 and 9 were ... | 1989 | 2734766 |
| hemodynamic response to pumpless extracorporeal membrane oxygenation. | | 1989 | 2736961 |
| antibiotic susceptibility of serotype 2 and 5 strains of actinobacillus (haemophilus) pleuropneumoniae isolated from swine from 1974 to 1986. | antibiotic susceptibility of 129 isolates of actinobacillus (haemophilus) pleuropneumoniae was examined. all the strains were isolated in 1974 to 1986 from the nasal cavities of swine housed on 35 farms in 11 prefectures of japan. all 28 strains of serotype 2 isolated before 1984 were susceptible to 10 antibiotics used. in contrast, more than one-half of 79 strains of serotype 2 isolated in 1985 and 1986 were resistant to aminoglycosides, tetracycline, tylosin and carbadox, and most of them show ... | 1989 | 2739211 |
| antimicrobial susceptibility of actinobacillus (haemophilus) pleuropneumoniae isolated from pigs with pleuropneumonia. | | 1989 | 2739226 |
| fluorodeoxyglucose rate constants in rat brain. | | 1989 | 2747927 |
| time-resolved extrinsic fluorescence of aromatic-l-amino-acid decarboxylase. | the coenzyme-linked fluorescence of aromatic-l-amino-acid decarboxylase decays non-exponentially. the decay of both native and nabh4 reduced samples can only be fitted by two exponentials each roughly accounting for about half of the total fluorescence. denaturation of the reduced protein with 8 m urea makes the fluorescence decay mono-exponential, like that observed for the reference compound pyridoxamine-5-phosphate. an extra pyridoxyl moiety can be bound to the enzyme after incubation with ex ... | 1989 | 2752045 |
| [yersiniosis]. | | 1989 | 2752462 |
| 131i-metaiodobenzylguanidine and 125i-iodoamphetamine. parameters of lung endothelial cell function and pulmonary vascular area. | radioiodinated metaiodobenzylguanidine (mibg) has been shown to be extracted in the lung by an active, sodium-dependent, saturable transport system similar to that which extracts norepinephrine. accordingly, first transit uptake of mibg has been investigated as a method for in vivo evaluation of pulmonary biogenic amine metabolism. issues that still must be resolved include the degree to which mibg extraction is affected by simple loss of available vascular surface (in distinction to changes in ... | 1989 | 2753053 |
| nucleotide sequence of 5s ribosomal rna of rhodococcus fascians. | | 1989 | 2762130 |
| nucleotide sequence of 5s ribosomal rna of rhodococcus fascians. | | 1989 | 2762130 |
| a simple time-resolved fluoroimmunoassay of total thyroxine in serum. | we describe a non-isotopic heterogeneous competitive immunoassay of total thyroxine in serum. thyroxine, released from its binding proteins by merthiolate (thimerosal), competes with immobilised thyroxine (thyroxine-bovine globulin conjugate) for binding to a monoclonal biotinylated antibody. the amount of biotinylated antibody bound, which is inversely related to the amount of thyroxine in the sample, is then quantified by adding streptavidin labelled with the europium chelator 4,7-bis(chlorosu ... | 1989 | 2764467 |
| in vitro antimicrobial activity of sulfonamides against some porcine pathogens. | the minimal inhibitory concentrations (mic) of sulfonamides were determined against bordetella bronchiseptica (n = 10), pasteurella multocida (n = 10), haemophilus pleuropneumoniae (n = 20), and streptococcus suis (n = 10) strains isolated from pigs with atrophic rhinitis, pneumonia, or meningitis. sulfonamides tested in an agar dilution method were sulfachloropyridazine, sulfadiazine, sulfadimethoxine, sulfamethazine, sulfadoxine, sulfisoxazole, sulfamerazine, sulfamethoxazole, sulfamethoxypyri ... | 1989 | 2774319 |
| [direct detection of actinobacillus (haemophilus) pleuropneumoniae in the lungs of swine]. | by means of cultural examination, coagglutination test (ct) and indirect fluorescent-antibody-technique (ifat) a total of 199 lung specimens from necropsy pigs from northwestern germany with symptoms of pleuropneumonia was examined for actinobacillus (haemophilus) pleuropneumoniae (ap). the ct was used to detect type specific antigens in lung extracts and the ifat was performed on tissue sections. both tests were found to be specific. detection and identification of ap by either test were succes ... | 1989 | 2776673 |
| distribution of homologies among the genomes of several actinophages of faenia and saccharopolyspora as determined by dna hybridization. | the dna of phi fr114, a temperate phage of the thermophilic actinomycete faenia rectivirgula, was hybridized with the genomes of 11 different phages of faenia and saccharopolyspora. this revealed several regions (modules) within the phi fr114 dna which were distributed independently among the genomes of the other phages. the genome of the lytic phage 121, originally described for sap. erythraea, exhibited a similar modular organization. so far no functions of the possible modules are known. | 1989 | 2777538 |
| opening remarks of president fernando e. agrait on the occasion of the 50th anniversary of the cayo santiago macaque colony. | | 1989 | 2780954 |
| a rapid method for detecting trichinella spiralis larvae in pork using a monoclonal antibody-latex conjugate. | a simple, non-microscopic method was developed for the direct detection of trichinella spiralis in pork. samples of meat were treated with pepsin for 2 h to liberate the larvae, and sonicates were made from the deposit and used in an antigen detection system. in this system, latex particles, sensitized with a parasite-specific monoclonal antibody, were utilized in a slide or tube agglutination test. the efficiencies of these immunological methods were compared with that of trichinelloscopy in th ... | 1989 | 2781716 |
| a rapid method for detecting trichinella spiralis larvae in pork using a monoclonal antibody-latex conjugate. | a simple, non-microscopic method was developed for the direct detection of trichinella spiralis in pork. samples of meat were treated with pepsin for 2 h to liberate the larvae, and sonicates were made from the deposit and used in an antigen detection system. in this system, latex particles, sensitized with a parasite-specific monoclonal antibody, were utilized in a slide or tube agglutination test. the efficiencies of these immunological methods were compared with that of trichinelloscopy in th ... | 1989 | 2781716 |
| human t lymphocytes possess a limited in vitro life span. | the t lymphocyte offers certain theoretical advantages over other available cell types for the study of aging. immunosenescence is a well-established part of, and may be directly relevant to, mammalian aging, and the t lymphocyte is well-characterized as to function, cell-surface antigen make-up, and other factors. however, prior efforts at studying in vitro aging of t cells have been hampered by poor reproducibility in doubling potential and the occurrence of a peculiar type of crisis. we have ... | 1989 | 2786475 |
| effect of cytokines and anti-arthritic drugs on glycosaminoglycan synthesis by bovine articular chondrocytes. | the effect of cytokines (interleukin-1 alpha, interleukin-1 beta, and tumor necrosis factor alpha) and several anti-arthritic drugs on glycosaminoglycan synthesis and secretion into medium by bovine articular chondrocytes was examined. sulfated glycosaminoglycans (s-gag) were measured by a modified 1,9-dimethylmethylene blue (dmb) dye binding assay. hyaluronate (ha) was measured by an inhibition elisa based on specific binding to a proteoglycan. all three cytokines caused a dose-dependent decrea ... | 1989 | 2801304 |
| purification and characterization of dihydropyrimidine dehydrogenase from pig liver. | dihydropyrimidine dehydrogenase was isolated from cytosolic pig liver extracts and purified 3100-fold to apparent homogeneity. purification made use of ammonium sulfate fractionation, precipitation with acetic acid and chromatography on deae-cellulose and 2',5'-adp-sepharose with 28% recovery of total activity. the native enzyme has a molecular mass of 206 kda and is apparently composed of two similar, if not identical, subunits. proteolytic cleavage reveals two fragments with apparent molecular ... | 1989 | 2806259 |
| biosynthesis, membrane translocation, and surface expression of sindbis virus e1 glycoprotein. | sindbis virus glycoproteins pe2 (precursor of e2) and e1 are coded in this order by the same monocistronic mrna, cotranslationally inserted in the endoplasmic reticulum membrane and then transported to the cell surface where the progeny virus is released by budding. in the virion, three e1 plus three e2 molecules form hexameric spike complexes. previous work (s. bonatti, g. migliaccio, g. blobel, and p. walter (1984), eur. j. biochem. 140, 499-502) revealed a single signal sequence for cotransla ... | 1989 | 2806407 |
| molecular cloning and characterization of a hemolysin gene from actinobacillus (haemophilus) pleuropneumoniae. | this article describes the molecular cloning and expression of a hemolysin gene from a serotype 1 strain of actinobacillus pleuropneumoniae. the hemolysin was a thermolabile protein with an apparent molecular weight of 29,500 (29.5k hemolysin). unlike expression of the recently described 105k hemolysin of a. pleuropneumoniae (j. frey and j. nicolet, fems microbiol. lett. 55:41-46, 1988), expression of this hemolysin was not regulated by ca2+. antiserum prepared against the 105k hemolysin did not ... | 1989 | 2807529 |
| structures and sugar compositions of lipopolysaccharides isolated from seven actinobacillus pleuropneumoniae serotypes. | highly purified lipopolysaccharide (lps) preparations obtained from seven actinobacillus pleuropneumoniae strains representative of seven different serotypes were used to determine the structure and monosaccharide composition of the polysaccharide components of each lipopolysaccharide. an indication of the structure of each lps was obtained by procedures that included sodium dodecyl sulfate-polyacrylamide gel electrophoresis followed by silver staining and gel chromatographic fractionation of ac ... | 1989 | 2807553 |
| [study of the possibility of breeding serologically negative animals from a breeding farm infected with serotype 2 actinobacillus pleuropneumoniae]. | two sanitation trials showed that it was possible to rear serologically negative animals from pigs on a breeding farm infected with actinobacillus pleuropneumoniae serotype 2. this was achieved by the medication of sows and piglets during the farrowing-house period and by isolating the piglets from the infected stock of pigs immediately after weaning. medication of the sows consisted in administering sulphamethoxazole-trimethoprim in the drinking water for a period from five days before to eight ... | 1989 | 2815050 |
| comparison of protective immunity and inflammatory responses of pigs following immunization with different actinobacillus pleuropneumoniae preparations with and without adjuvants. | three experiments were performed to evaluate the inflammatory response, the antibody response and protection from experimental challenge of various actinobacillus pleuropneumoniae serotype 5 (ap5) vaccines in swine. in the first experiment, subcutaneous injections of either a water-in-oil (w/o) emulsion or freund's complete adjuvant (fca) caused lesions at the site of injection, while intraperitoneal injection of the w/o emulsion caused no lesions. in the second experiment, intraperitoneal (ip) ... | 1989 | 2815578 |
| actinobacillus (haemophilus) pleuropneumoniae infections in swine: serological investigations and vaccination trials in combination with environmental improvements. | the present investigation comprised six integrated herds (100-150 sows each) and one specialized fattening herd. serologic tests by elisa were performed on blood samples from piglets and growing pigs during various rearing phases in order to determine the presence of actinobacillus infection and to estimate the age of the animals at onset of the disease. using this information, herd-specific vaccination programs were designed against actinobacillus pleuropneumoniae serotype 2. the weaned piglets ... | 1989 | 2816173 |
| cloning, sequence, and expression of the drosophila camp-dependent protein kinase catalytic subunit gene. | genomic dna containing the protein coding region for drosophila camp-dependent protein kinase catalytic subunit has been cloned and sequenced. the probe used to detect and isolate the gene fragment was constructed from two partially complementary synthetic oligonucleotides and contains 60 base pairs that encode (using drosophila codon preferences) amino acids 195-214 of the beef heart catalytic subunit. in reduced stringency hybridization conditions, the probe recognizes two target sites in fly ... | 1988 | 2828348 |
| cloning, sequence, and expression of the drosophila camp-dependent protein kinase catalytic subunit gene. | genomic dna containing the protein coding region for drosophila camp-dependent protein kinase catalytic subunit has been cloned and sequenced. the probe used to detect and isolate the gene fragment was constructed from two partially complementary synthetic oligonucleotides and contains 60 base pairs that encode (using drosophila codon preferences) amino acids 195-214 of the beef heart catalytic subunit. in reduced stringency hybridization conditions, the probe recognizes two target sites in fly ... | 1988 | 2828348 |
| interaction between highly selective mu and delta opioids in vivo at the rat spinal cord. | possible interactions between mu- and delta-receptors in the rat spinal cord were studied using the radiant-heat-induced tail flick response and the highly selective mu- or delta-ligands: [nmephe3,d-pro4] morphiceptin(pl-17) and cyclic[d-pen2,d-pen5]enkephalin(dpdpe). intrathecal infusions of pl-17 (0.5 microgram/hr) for 5 days caused 61- and 6-fold shifts to the right of the dose-response curves for pl-17 and dpdpe, respectively. since pl-17 and dpdpe are highly selective agonists for mu- and d ... | 1986 | 2829017 |
| characterization of temperature-sensitive mutants mouse hepatitis virus type 3. | | 1987 | 2829537 |
| kirsten murine sarcoma virus abolishes interferon gamma-induced class ii but not class i major histocompatibility antigen expression in a murine fibroblast line. | the effect of infecting fibroblasts with kirsten murine sarcoma virus/murine leukemia virus (ki-msv/mlv) on constitutive and ifn-gamma-induced h-2 antigen expression was investigated. the fibroblasts used were two established cell lines (c3h10t1/2 and balb/c3t3) and fresh embryo fibroblasts from c3h mice. class i antigens were expressed constitutively by balb/c3t3; infection with mlv, msv or the two together had little effect on this constitutive expression. class i antigens (h-2k, h-2d) were st ... | 1988 | 2831293 |
| influence of immunomodulatory agents on bovine humoral and cellular immune responses to parenteral inoculation with bovine rotavirus vaccines. | sodium diethyldithiocarbamate (dtc), mycobacterium cell wall extract (mcwe, regressin), killed corynebacterium parvum (c. parvum, immunoregulin) and muramyldipeptide (mdp) were each combined with purified, live bovine rotavirus and inoculated into 3 month-old holstein-friesian calves in order to examine their ability to potentiate specific humoral and cellular immune responses. the vaccinated calves were boosted twice at 3 and 6 weeks after initial vaccine inoculation. the rotavirus was administ ... | 1988 | 2847397 |
| expression of env sequences of the bovine leukemia virus (blv) in the yeast saccharomyces cerevisiae. | dna sequences of the envelope (env) gene of the bovine leukemia virus (blv) were expressed in the yeast saccharomyces cerevisiae. two yeast promoters, the repressible pho5 promoter and the constitutive pgk promoter, were used to construct four expression plasmids comprising either a sequence of the surface antigen gp51 or a (gp51 + gp30) sequence. the expressed heterologous gene products were characterized by western blot analysis and competitive radioimmunoassay. by means of northern blot analy ... | 1988 | 2849260 |
| suppression of sv40-promoted gene expression by differentiation of preadipose cells. | when a plasmid bearing the chloramphenicol acetyltransferase (cat) gene under the control of an sv40 early promoter is introduced into preadipose or adipose cells of line 3t3-f442a, the promoter directs high levels of transient expression of cat. however, when the plasmid is introduced into preadipose cells and the cells are then allowed to differentiate into adipose cells, the expression of the cat gene is suppressed. in this process, the plasmid is not changed detectably in amount, topology, o ... | 1988 | 2849579 |
| phorbol ester and dibutyryl cyclic amp reduce content and efflux of taurine in primary cerebellar astrocytes in culture. | in 16-18 days in vitro (div) primary astrocyte cultures prepared from 7- to 9-day-old rats, 48 h exposure to 12,13-phorbol dibutyrate (pdbu) (1 microm) or dibutyryl camp (dbcamp) (1 mm) reduced cellular taurine content, and both basal and 50 mm k+-evoked taurine efflux, but did not alter cellular glutamate or total protein content. decreases in cellular taurine content first became apparent between 1 and 6 h and were maximal after 24 h. treatment also rapidly altered astrocyte morphology to a mo ... | 1988 | 2852322 |
| comparative effects of adimolol, labetalol and propranolol on heart rate and blood pressure in man. | the cardiovascular effects of adimolol, a new, potent, long acting beta-adrenoceptor blocking drug were investigated in three studies. in the first study blood pressure and heart rate were measured in five male volunteers before and at 2, 4, 6, 8, 24, 32, 48, 72 and 96 h after single oral doses of adimolol. all doses of adimolol reduced supine, standing and exercise heart rates in a dose dependent manner. the maximum effect ranged from 18% following 25 mg to 29% following 600 mg and all doses sh ... | 1985 | 2859871 |
| entamoeba histolytica, antigens and amoebiasis. | | 1985 | 2866068 |
| regulation of kidney calcium-binding protein in the bird (gallus domesticus). | the possible involvement of plasma calcium and 1,25-dihydroxyvitamin d3 [1,25(oh)2d3] in the regulation of the concentration of kidney calcium-binding protein (cabp) was investigated. chicks were fed diets varying in ca2+ and p, with or without vitamin d. cabp and 1,25(oh)2d3 were determined by competitive binding assays. a significant correlation between plasma and kidney 1,25(oh)2d3 was found, the linear regression equation of best-fit was plasma 1,25(oh)2d3 = 0.14 + 1.56 kidney 1,25(oh)2d3. i ... | 1986 | 2869867 |
| chlamydia in women: a case for more action? | the 1985 communicable disease surveillance center figures for sexually transmitted diseases document over 14,000 confirmed cases of genital chlamydial infection in women. yet, this figure seriously underestimates the size of the problem as many chlamydial infections are silent. the mainstay of diagnosis until recently has been isolation of c. trachomatis in cell culture, which is time consuming, technically demanding, expensive, and available in only a few centers. a firm diagnosis of chlamyd ... | 1986 | 2870360 |
| chlamydia in women: a case for more action? | the 1985 communicable disease surveillance center figures for sexually transmitted diseases document over 14,000 confirmed cases of genital chlamydial infection in women. yet, this figure seriously underestimates the size of the problem as many chlamydial infections are silent. the mainstay of diagnosis until recently has been isolation of c. trachomatis in cell culture, which is time consuming, technically demanding, expensive, and available in only a few centers. a firm diagnosis of chlamyd ... | 1986 | 2870360 |
| a glia-derived neurite promoting factor with protease inhibitory activity belongs to the protease nexins. | a glia-derived neurite promoting factor (gdnpf) has serine protease inhibitory activity and in addition regulates the migration of neuronal cells. cdna cloning of gdnpf is necessary for studying the physiological relevance and the mode of action of this protein and similar cell-derived protease inhibitors. xenopus oocytes injected with rat glioma cells mrna release this inhibitor. a rat cdna clone coding for the previously purified glia-derived neurite promoting factor (gdnpf) was isolated upon ... | 1986 | 2877744 |
| neuropeptides trh and cyclo(his-pro) share neuromodulatory, but not stimulatory, action on hypothalamic neurons in vitro: implication for the regulation of feeding. | electrical activity of neurons in rat hypothalamic ventromedial nucleus was recorded in tissue slices, to investigate central neural mechanisms underlying reduction of food intake caused by trh and its metabolite, cyclo(his-pro) [chp]. application of trh had two actions: stimulation of neuronal activity, which was desensitized on closely repeated applications; and modulation of neuronal responses to neurotransmitters, even in the absence of the stimulatory action. the neuromodulatory but not the ... | 1987 | 2887450 |
| restriction enzyme maps of the macropodid herpesvirus 2 genome. | the dna of macropodid herpesvirus 2 (dorcopsis wallaby herpesvirus) was analysed using restriction enzymes and molecular cloning techniques. sets of ecori and sali clones were prepared which represented approximately 85 per cent of the genome, and these clones were used to map the dna by double-digestion and hybridization experiments. data on uncloned regions were obtained by analysing fragments excised from agarose gels, and terminal fragments were identified by exonuclease iii digestion. the g ... | 1987 | 2889444 |
| the high molecular weight receptor to transforming growth factor-beta contains glycosaminoglycan chains. | proteoglycans are constituents of the cell surface that may play important roles in the regulation of cell behavior. here we report that the 250-kda receptor subunit that binds the multifunctional protein, transforming growth factor-beta 1 (tgf-beta 1), contains chains of heparan sulfate and chondroitin sulfate and thus is a proteoglycan. digestion of tgf-beta 1-receptor complexes with glycosaminoglycan (gag)-specific degradative enzymes yield core proteins of 115-140 kda. cell monolayers that h ... | 1988 | 2897367 |
| plasmid mediated antimicrobial resistance in ontario isolates of actinobacillus (haemophilus) pleuropneumoniae. | the genetic basis of antimicrobial resistance in ontario isolates of actinobacillus (haemophilus) pleuropneumoniae was studied. two ontario isolates of a. pleuropneumoniae were found to be resistant to sulfonamides (su), streptomycin (sm) and ampicillin (amp). resistance to su and sm was specified by a 2.3 megadalton (mdal) plasmid which appeared to be identical to pvm104, which has been described in isolates of a. pleuropneumoniae from south dakota. southern hybridization showed that the 2.3 md ... | 1989 | 2914226 |
| blood gas stability and hematological changes in experimentally-induced acute porcine pleuropneumonia. | blood gas and hematological responses to acute, mild actinobacillus pleuropneumoniae infection of growing pigs was studied. six pigs (average weight 10.1 kg) were experimentally infected intranasally with a. pleuropneumoniae serotype 5. four pigs served as controls. rectal temperatures and arterial blood for gas analysis and hematology were taken at 0, 8, 16, 24, 48 and 72 h postinfection. all infected pigs became febrile showing clinical signs typical of mild to moderate porcine pleuropneumonia ... | 1989 | 2914231 |
| one of two subunits of masking protein in latent tgf-beta is a part of pro-tgf-beta. | a high molecular mass latent form of transforming growth factor type-beta (tgf-beta) was purified to homogeneity from rat platelets by a seven-step procedure involving group-specific affinity chromatographies on red-toyopearl and zinc chelating-sepharose. the purified latent tgf-beta was a complex of tgf-beta (25 kda) and the binding protein previously named masking protein (approximately 400 kda) [(1986) biochem. biophys. res. commun. 141, 176-184]. analysis of the peptide structure by gel elec ... | 1989 | 2914605 |
| epiphyseal lesions of the femur and tibia in rats following oral chronic administration of zinc dimethyldithiocarbamate (ziram). | a 24-month chronic feeding toxicity study with zinc dimethyldithiocarbamate (ziram) was performed on fischer 344 rats of both sexes (80 animals/sex per group) at dietary levels of 0, 20, 200, or 2000 ppm. eight animals of either sex from each group were sacrificed after 26, 52 and 78 weeks, and all surviving animals were killed after 104 weeks. epiphyseal abnormalities in the long bones of the hind legs were observed in both sexes at 2000 ppm. clinically, 3 male rats showed partial paralysis of ... | 1989 | 2916241 |
| effect of iron restriction on the outer membrane proteins of actinobacillus (haemophilus) pleuropneumoniae. | the outer membrane protein profile of actinobacillus (haemophilus) pleuropneumoniae grown under iron-restricted and iron-replete conditions was studied by polyacrylamide gel electrophoresis and immunoblotting. a virulent serotype 1 isolate synthesized a novel protein with an apparent molecular weight of 105,000 (105k) and increased the synthesis of a 76k protein under iron-restricted conditions. both proteins were synthesized within 15 min of establishment of iron-restricted conditions. proteins ... | 1989 | 2917787 |
| characterisation and antimicrobial sensitivity of haemophili isolated from pigs. | a total of 70 haemophili from australia pigs was compared with a range of reference strains of porcine haemophili. forty-eight of the isolates were identified as actinobacillus pleuropneumoniae biovar 1 and the remaining 22 isolates as haemophilus parasuis. forty one of the a. pleuropneumoniae isolates were used in a study to determine to the minimal inhibitory concentration (mic) of 12 antimicrobial agents, or combinations of agents. penicillin, neomycin, trimethoprim, trimethoprim-sulphamethox ... | 1989 | 2930386 |
| biochemical and cytochemical comparison of intestinal bicarbonate-stimulated mg2+ dependent atpase and alkaline phosphatase activities in rat, rabbit and guinea pig. | bicarbonate-stimulated mg2+ dependent atpase activity was demonstrated both biochemically and cytochemically, in brush border membranes from rat, rabbit and guinea pig duodenum. there was no correlation between enzyme activity and basal hco3- secretion rates in the different species. the concentration of hco3- necessary for optimal stimulation of atpase activity, degree of stimulation and total activity was higher in the rat than in other species. activity was higher in rat duodenum than in the ... | 1987 | 2953698 |
| umbilical artery flow velocity waveforms and placental resistance: the effects of embolization of the umbilical circulation. | embolization of the umbilical placental circulation in fetal lambs was carried out to occlude the small vessels of the placental vascular bed and to observe the effect on the umbilical artery flow and flow velocity waveforms. thirteen singleton fetuses were studied from day 120 of pregnancy. embolization was achieved by injecting approximately 9 x 10(6) microspheres of 15 micron diameter into the fetal placental cotyledons along the umbilical arteries over 9 days. umbilical and uterine flows wer ... | 1987 | 2962497 |
| potentiation of the antitumor effect of 5-fluoro-2'-deoxyuridine esters in combination with acyclothymidine esters on l1210 in mice via oral administration. | fifteen pyrimidine-related compounds were evaluated for their ability to inhibit enzymatic degradation of 5-fluoro-2'-deoxyuridine (fudr). acyclothymidine [5-methyl-1-(2'-hydroxyethoxymethyl)uracil] showed the highest inhibitory effect on the phosphorolytic degradation of fudr in various tissue homogenates derived from mouse, rat, and beagle organs. both the drug (fudr) and the inhibitor (acyclothymidine) were esterified with appropriate aliphatic acids in order to synchronize their behavior aft ... | 1988 | 2976090 |
| effect of atrial peptide on collecting duct function. | 1. the effects of synthetic rat atrial natriuretic peptides (anp) on diffusional 22na+, 36cl- and tritiated water (tho) permeability of in vitro microperfused rat papillary collecting ducts and the effect in vivo of anp on stop-flow sodium concentrations in the terminal segment of rabbit nephrons were studied. 2. the addition of 4 x 10(-8) or 4 x 10(-7) mol/l anp to the medium or perfusion solution did not alter diffusional 22na+ or 36cl- permeability of microperfused rat papillary collecting du ... | 1988 | 2976327 |
| [rpo pathway as a possible recombination mechanism of the interaction between dna of transducing phages and the escherichia coli genome]. | the conditions affecting recombination of dnas of transducing lambdoid rifd phages with the chromosome of escherichia coli k-12 reca in the region of homology were studied. in support to the previously obtained data, the int system was shown to take no part in the process. the homologous character of recombination interactions and their dependence on efficiency of transcription were demonstrated. it is therefore suggested that recombination takes the rpo pathway. similar peculiarities were revea ... | 1988 | 2976700 |
| effect of melphalan on growth curves and cell cycle distribution of four human small cell carcinomas of the lung grown in nude mice. | four human small cell carcinomas of the lung grown in nude mice were exposed to melphalan. two of the tumors were derived from subpopulations isolated by in vitro cloning from the same tumor biopsy. the chemosensitivity of the tumors was determined by calculating the specific growth delay. drug-induced changes in the cell cycle were detected by flow cytometric dna analysis. the specific growth delay of the tumors was very different with the greatest differences between the two subpopulations ori ... | 1986 | 3017779 |
| inositol phosphate production following alpha 1-adrenergic, muscarinic or electrical stimulation in isolated rat heart. | a possible participation of polyphosphoinositide metabolism in the excitation-contraction coupling in heart was investigated. isolated rat ventricles prelabelled with myo-[2-3h]inositol were stimulated by conditions that increase mechanical activity. both noradrenaline and carbachol increased the basal level of ip3, ip2 and ip by the activation of alpha 1-adrenergic and muscarinic receptors, respectively. electrical stimulation accelerated inositol lipid degradation by phospholipase c thus enhan ... | 1986 | 3019774 |
| inositol phosphate production following alpha 1-adrenergic, muscarinic or electrical stimulation in isolated rat heart. | a possible participation of polyphosphoinositide metabolism in the excitation-contraction coupling in heart was investigated. isolated rat ventricles prelabelled with myo-[2-3h]inositol were stimulated by conditions that increase mechanical activity. both noradrenaline and carbachol increased the basal level of ip3, ip2 and ip by the activation of alpha 1-adrenergic and muscarinic receptors, respectively. electrical stimulation accelerated inositol lipid degradation by phospholipase c thus enhan ... | 1986 | 3019774 |