Publications

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report of a visit to the people's republic of china. a norwegian's diary. 20154515715
[perinatal death in the women's hospital in groningen]. 20165028747
[changes of the hematocrit in balb/c mice in the adaptation to hypoxia in high altitude]. 20164807525
[clinico-roentgenological characteristics of metatuberculous atelectatic pneumosclerosis]. 20164772473
[dynamics of the accumulation of essential oil and menthol in peppermint of the "polyhybrid-7" variety]. 20154417705
electrophysiological effects of mexiletine in patients of sinus node dysfunction and intraventricular conduction defects. 20123557516
electrophysiological effects of mexiletine in patients of sinus node dysfunction and intraventricular conduction defects. 20123557516
michigan doctor encourages children to plant trees, help environment. 20062586341
homogeneous immunoglobulins in the serum of irradiated and bone marrow reconstituted mice: the role of thymus and spleen.the influence of thymectomy and splenectomy on the frequency and class distribution of homogeneous immunoglobulins (h-ig) in serum was studied in lethally irradiated (dba/2 x c57bl/rij)f1 mice reconstituted with syngeneic bone marrow. during four follow-up periods in the first 9 months after transplantation, the sham-operated controls and splenectomized animals developed transient h-ig in an average frequency of 14.2 and 15.7% respectively. there were no marked differences in the incidence of h- ...2004387578
[enterostoma therapy--a pilot course in the essen education center of the german nursing association]. 20113127632
osteosarcoma. 1980291463
the effect of ozone on pentobarbital sleeping time in mice. 20154456501
gamma-d paraproteinemia - a report of 16 cases.paraproteins of the gammad class are very rare, being found in only 0.3% to 3.0% of the cases of all paraproteinemias. they are difficult to detect and therefore sometimes not found or mis-diagnosed. in the 6500 cases of paraproteinemia we have diagnosed over the last eight years, 16 of them have been gammad. consistent with the reports of other gammad paraproteinemias most of these 16 cases were of type l (13), most patients were male (11), the average age (55), was younger than that of patient ...199150964
acute renal failure secondary to intravenous pyelography in a child. 20123347914
ophthalmopathy of graves' disease. outcome after treatment with radioactive iodine, surgery, or antithyroid drugs. 20144113850
[familial myocardial diseases: clinical features and postpericardiotomy]. 20154475702
[primary projection of receptors of extrinsic ocular muscles: anatomical results].recently, several electrophysical studies have shown that extraocular sensory afferents projected onto subcortical nuclei and cortical area as well. in a recent study of the projection fields of the primary afferent fibers from extraocular muscles, it has been shown that the intermediate layers of the cervical dorsal horn of the spinal cord was one of the recipient zone.20071951928
biology of the dental pulp. 1980291327
thumb reconstruction after amputation at the interphalangeal joint by gradual lengthening of the proximal phalanx. a case report.a case of thumb amputation at the interphalangeal joint and reconstruction by gradual lengthening of the proximal phalanx is described. as a result of the treatment the amputation stump was lengthened 2cms which means a 70 per cent increase of the original length of the proximal phalanx. the quality of sensibility of the stump has been preserved.2004520875
[significance and classification of bronchial asthma]. 20123682201
[significance and classification of bronchial asthma]. 20123682201
[the measurement of the edema occurring after the removal of impacted or semi-impacted wisdom teeth]. 20133870099
volcanoes and occupational health. 20133707865
[determination of the degree of prethrombotic state by the thrombophilia index]. 20144409155
[determination of the degree of prethrombotic state by the thrombophilia index]. 20144409155
prevention of neonatal intraventricular hemorrhage. 20143990738
intermittent delta rhythms in nocturnal sleep. 20144183612
[immunologic phenomena in experimental toxoplasmosis. i. in vitro study in balb-c mice]. 20154661613
side-effect of topical thio-tepa. 20164980007
the role of revolutionary optimism in the treatment of mental illness in the people's republic of china. 20164742815
clinical pathology teaching. 20164711142
microbiological evolution of gilthead sea bream (sparus aurata) in canary islands during ice storage.this study analyses the microbiological changes with traditional methods for total mesophilic aerobic, psychrotrophic, aeromonas sp., pseudomonas sp., shewanella putrefaciens, enterobacteriaceae, sulfide-reducing clostridium and photobacterium phosphoreum in muscle, skin and gills of whole ungutted gilthead sea bream (sparus aurata) stored in ice during 18 days. the muscle tissue showed the minor grade of contamination, followed by the skin and the gills, with statistic significance (p < 0.001). ...201325745228
development of a rapid real-time pcr method as a tool to quantify viable photobacterium phosphoreum bacteria in salmon (salmo salar) steaks.a specific real-time pcr quantification method combined with a propidium monoazide sample treatment step was developed to determine quantitatively the viable population of the photobacterium phosphoreum species group in raw modified-atmosphere-packed salmon. primers were designed to amplify a 350-bp fragment of the gyrase subunit b gene (gyrb) of p. phosphoreum. the specificity of the two primers was demonstrated by using purified dna from 81 strains of 52 different bacterial species. when these ...201323396343
population dynamics of vibrio and pseudomonas species isolated from farmed tasmanian atlantic salmon (salmo salar l.): a seasonal study.vibrio and pseudomonas species have been shown to be part of the normal microbiota of atlantic salmon (salmo salar l.), with some strains causing disease in fish. the factors affecting their prevalence and persistence in the salmon gut, however, have not been well studied. in this study, we collected 340 vibrio and 150 pseudomonas isolates from the hindgut of farmed tasmanian atlantic salmon, fed with two commercially available diets. samples were collected every 6-8 weeks between july 2011 and ...201425027277
[comparative sensitivity of the luminescent photobacterium phosphoreum, escherichia coli, and bacillus subtilis strains to toxic effects of carbon-based nanomaterials and metal nanoparticles].a comparative analysis of the four commercially available and laboratory luminescent sensor strains to the toxic effect of 10 carbon-based nanomatherials (cbns) and 10 metal nanoparticles (mnps) was carried out in this study. the bioluminescence inhibition assays with marine photobacterium phosphoreum and recombinant escherichia coli strains were varied in minimal toxic concentrations and ec50 values but led to well correlated biotoxicity evaluation for the most active compounds were ranked as c ...201627476206
ecotoxicity monitoring and bioindicator screening of oil-contaminated soil during bioremediation.a series of toxicity bioassays was conducted to monitor the ecotoxicity of soils in the different phases of bioremediation. artificially oil-contaminated soil was inoculated with a petroleum hydrocarbon-degrading bacterial consortium containing burkholderia cepacia gs3c, sphingomonas gy2b and pandoraea pnomenusa gp3b strains adapted to crude oil. soil ecotoxicity in different phases of bioremediation was examined by monitoring total petroleum hydrocarbons, soil enzyme activities, phytotoxicity ( ...201626491984
impact of nisin-activated packaging on microbiota of beef burgers during storage.beef burgers were stored at 4°c in a vacuum in nisin-activated antimicrobial packaging. microbial ecology analyses were performed on samples collected between days 0 and 21 of storage to discover the population diversity. two batches were analyzed using rna-based denaturing gradient gel electrophoresis (dgge) and pyrosequencing. the active packaging retarded the growth of the total viable bacteria and lactic acid bacteria. culture-independent analysis by pyrosequencing of rna extracted directly ...201526546424
selection of bioprotective cultures for preventing cold-smoked salmon spoilage.biopreservation is a natural technology of food preservation, which consists of inoculating food with microorganisms selected for their antibacterial properties. the objective of this study was to select lactic acid bacteria (lab) to improve the quality of cold-smoked salmon (css). in this work, different strains representative of the 4 dominant species, identified in a previous study by pyrosequencing the 16s rrna gene, were isolated and their spoiling potential in css blocks, sterilized by ion ...201526044337
the effect of crowding stress on bacterial growth and sensory properties of chilled atlantic salmon fillets.atlantic salmon were subjected to minimal preslaughter crowding stress (control), short-term crowding for 20 min (ss-group), or long-term crowding for 24 h (ls-group). the fish were filleted prerigor, cut into 270 g pieces, and packaged in modified atmosphere (60% co₂ and 40% n₂). fillet quality analyses were determined during 22 d of storage at 0.3 °c. bacterial growth and unpleasant sensory properties increased earlier in the ls-group. the negative effects of long-term preslaughter stress were ...201222260135
a zeta potential value determines the aggregate's size of penta-substituted [60]fullerene derivatives in aqueous suspension whereas positive charge is required for toxicity against bacterial cells.the cause-effect relationships between physicochemical properties of amphiphilic [60]fullerene derivatives and their toxicity against bacterial cells have not yet been clarified. in this study, we report how the differences in the chemical structure of organic addends in 10 originally synthesized penta-substituted [60]fullerene derivatives modulate their zeta potential and aggregate's size in salt-free and salt-added aqueous suspensions as well as how these physicochemical characteristics affect ...201526253116
sensitivity to pulsed light technology of several spoilage and pathogenic bacteria isolated from fish products.the effect of pulsed light (pl) on the inactivation of six fish spoilage bacteria (photobacterium phosphoreum, serratia liquefaciens, shewanella putrefaciens, brochothrix thermosphacta, pseudomonas group i, and pseudomonas groups iii and iv), six listeria monocytogenes isolates from fish products, and one strain of listeria innocua was evaluated. for all tested strains, grown at 4°c (temperature to process, store, and distribute convenient, lightly preserved fish products), the maximum detectabl ...201223127714
paraphotobacterium marinum gen. nov., sp. nov., a member of the family vibrionaceae, isolated from surface seawater.a novel gram-stain-negative bacterium, named strain nscs20n07dt, was isolated from surface seawater of the south china sea. cells of this strain contained poly-β-hydroxybutyrate granules. growth was observed at 15-35 °c with optimum of 30 °c, at a salinity range of 1-6 % (w/v) nacl with optimum of 3 % and at ph 5-8 with optimum of ph 5. the full-length 16s rrna gene sequence of strain nscs20n07dt showed highest similarity to photobacterium iliopiscarium atcc 51760t of 96.0 %, followed by photoba ...201627154455
genetic diversity analysis of isolates belonging to the photobacterium phosphoreum species group collected from salmon products using aflp fingerprinting.an accurate amplified fragment length polymorphism (aflp) method, including three primer sets for the selective amplification step, was developed to display the phylogenetic position of photobacterium isolates collected from salmon products. this method was efficient for discriminating the three species photobacterium phosphoreum, photobacterium iliopiscarium and photobacterium kishitanii, until now indistinctly gathered in the p. phosphoreum species group known to be strongly responsible for se ...201626513249
photobacterium piscicola sp. nov., isolated from marine fish and spoiled packed cod.five isolates from marine fish (w3(t), wm, w1s, s2 and s3) and three isolates misclassified as photobacterium phosphoreum, originating from spoiled modified atmosphere packed stored cod (ncimb 13482 and ncimb 13483) and the intestine of skate (ncimb 192), were subjected to a polyphasic taxonomic study. phylogenetic analysis of 16s rrna gene sequences showed that the isolates were members of the genus photobacterium. sequence analysis using the gapa, gyrb, pyrh, reca and rpoa loci showed that the ...201424951451
soil properties and microbial ecology of a paddy field after repeated applications of domestic and industrial sewage sludges.the effects of repeated application of two types of sewage sludge, domestic and industrial (petrochemical, pss) sludges, into paddy fields over a 5-year period on the soil properties and microbial ecology were studied and compared with conventional npk fertilizer application. soil organic matter and total nitrogen contents were significantly higher in the two sludge treatments than that in fertilized plots after 5 years. soil concentrations of potentially toxic metals were low after 5 years of b ...201728194679
exposure of luminous marine bacteria to low-dose gamma-radiation.the study addresses biological effects of low-dose gamma-radiation. radioactive (137)cs-containing particles were used as model sources of gamma-radiation. luminous marine bacterium photobacterium phosphoreum was used as a bioassay with the bioluminescent intensity as the physiological parameter tested. to investigate the sensitivity of the bacteria to the low-dose gamma-radiation exposure (≤250 mgy), the irradiation conditions were varied as follows: bioluminescence intensity was measured at 5, ...201728086187
acute toxicity reduction and toxicity identification in pigment-contaminated wastewater during anaerobic-anoxic-oxic (a/a/o) treatment process.in china, a considerable part of industrial wastewater effluents are discharged into the municipal wastewater treatment plants (wwtps) after pretreatment in their own wastewater treatment plants. even though the industrial effluents meet the professional emission standards, many micro-pollutants still remained, and they could be resistant in the municipal wwtps with conventional activated sludge process. pigment wastewater was chosen in this study, and the acute toxicity reduction and identifica ...201727919537
a chemometrics approach applied to fourier transform infrared spectroscopy (ftir) for monitoring the spoilage of fresh salmon (salmo salar) stored under modified atmospheres.the aim of this work was to investigate the potential of fourier transform infrared spectroscopy (ftir) to detect and predict the bacterial load of salmon fillets (salmo salar) stored at 3, 8 and 30°c under three packaging conditions: air packaging (ap) and two modified atmospheres constituted by a mixture of 50%n2/40%co2/10%o2 with lemon juice (mapl) and without lemon juice (map). fresh salmon samples were periodically examined for total viable counts (tvc), specific spoilage organisms (sso) co ...201727838518
acute toxicity assessment of explosive-contaminated soil extracting solution by luminescent bacteria assays.explosive-contaminated soil is harmful to people's health and the local ecosystem. the acute toxicity of its extracting solution was tested by bacterial luminescence assay using three kinds of luminescent bacteria to characterize the toxicity of the soil. an orthogonal test l 16 (4(5)) was designed to optimize the soil extracting conditions. the optimum extracting conditions were obtained when the ultrasonic extraction time, ultrasonic extraction temperature, and the extraction repeat times were ...201627566156
eco-toxicological bioassay of atmospheric fine particulate matter (pm2.5) with photobacterium phosphoreum t3.a bioluminescent bacterium, photobacterium phosphoreum t3 (ppt3), was used as a bio-indicator for the atmospheric fine particulate matter (pm2.5) to determine the eco-toxicity of pm2.5. the pm2.5 contains toxic chemicals, which reduce light output. the pm2.5 samples were collected in the period from march 2014 to january 2015 in nanjing and analyzed for the chemical composition versus their eco-toxicity. the eco-toxicological responses of each toxicant were detected in pm2.5 samples with ppt3. t ...201627467023
oxidative degradation of triclosan by potassium permanganate: kinetics, degradation products, reaction mechanism, and toxicity evaluation.in this study, we systematically investigated the potential applicability of potassium permanganate for removal of triclosan (tcs) in water treatment. a series of kinetic experiments were carried out to study the influence of various factors, including the ph, oxidant doses, temperature, and presence of typical anions (cl(-), so4(2-), no3(-)), humic acid (ha), and fulvic acid (fa) on triclosan removal. the optimal reaction conditions were: ph = 8.0, [tcs]0:[kmno4]0 = 1:2.5, and t = 25 °c, where ...201627459151
joint toxicity of two phthalates with waterborne copper to daphnia magna and photobacterium phosphoreum.di-n-butyl phthalate (dbp) and di-2-ethylhexyl phthalate (dehp) are two widely used phthalates, while cu(ii) is a common valence state of copper. they have been ubiquitously detected in the aquatic environment, but information on their joint toxicity to aquatic organisms is scarce. in this study, we evaluated the combined effects of copper and these two phthalates to daphnia magna and photobacterium phosphoreum by quantifying the acute toxicity expressed by the ec50 (the concentration causing 50 ...201627385371
environmental quality in sediments of cadiz and algeciras bays based on a weight of evidence approach (southern spanish coast).this research applies an integrated sediment quality assessment method using a weight of evidence approach to cadiz and algeciras bays (southern spain). the method is composed of several analyses (particle size profile, aqua regia extractable metals, acid labile metals, total organic carbon, toxicity bioassay with photobacterium phosphoreum and macrobenthic community alteration). the proposed method provides a single result, the environmental degradation index (edi). edi defined samples as low d ...201627371957
decolorization of azo dye c.i. reactive black 5 by ozonation in aqueous solution: influencing factors, degradation products, reaction pathway and toxicity assessment.in this study, ozonation treatment of c.i. reactive black 5 (rb5) was investigated at various operating parameters. the results showed that the aqueous solution initially containing 200 mg/l rb5 was quickly decolorized at ph 8.0 with an ozone dose of 3.2 g/h. reaction intermediates with m/z 281, 546, 201, 350, 286 and 222 were elucidated using liquid chromatography-mass spectrometry, while sulfate ion, nitrate ion and three carboxylic acids (i.e., oxalic acid, formic acid, and acetic acid) were ...201627054721
genomic and physiological analysis reveals versatile metabolic capacity of deep-sea photobacterium phosphoreum ant-2200.bacteria of the genus photobacterium thrive worldwide in oceans and show substantial eco-physiological diversity including free-living, symbiotic and piezophilic life styles. genomic characteristics underlying this variability across species are poorly understood. here we carried out genomic and physiological analysis of photobacterium phosphoreum strain ant-2200, the first deep-sea luminous bacterium of which the genome has been sequenced. using optical mapping we updated the genomic data and r ...201627039108
transformation of benzophenone-type uv filters by chlorine: kinetics, products identification and toxicity assessments.the present study focused on the kinetics, transformation pathways and toxicity of several benzophenone-type ultraviolet filters (bps) during the water chlorination disinfection process. the transformation kinetics of the studied three bps was found to be second-order reaction, which was dependent on the concentration of bps and chlorine. the second-order rate constants increased from 86.7 to 975 m(-1) s(-1) for oxybenzone, 49.6-261.7 m(-1) s(-1) for 4-hydroxybenzophenone and 51.7-540 m(-1) s(-1 ...201627035274
the investigation of different pollutants and operation processes on sludge toxicity in sequencing batch bioreactors.the influence of different target pollutants and operation modes in sequencing batch bioreactors (sbrs) on sludge toxicity was compared in this study. sludge toxicity was characterized by the inhibiting luminosity through using luminescent bacterium photobacterium phosphoreum (p. phosphoreum) during either gradual acclimation or impaction processes with synthetic wastewater containing high-strength bisphenol a (bpa) or n, n-dimethylformamide (dmf). when the activated sludge was first acclimated ...201626914341
biosensitive element in the form of immobilized luminescent photobacteria for detecting ecotoxicants in aqueous flow-through systems.we demonstrated the possibility of long-term and efficient application of a biosensitive element (be) in the form of photobacterium phosphoreum photobacteria immobilized in poly(vinyl alcohol) (pva) cryogel for detecting various ecotoxicants (zn(2) (+) , cu(2) (+) , hg(2) (+) , pb(2) (+) , 2,4-dichlorophenoxyacetic acid, 2,6-dimethylphenol, pentachlorophenol, coumaphos, malathion, chlorpyrifos and methyl parathion) in flow-through media. the range of detectable concentrations of ecotoxicants was ...201626864478
ozonation of the uv filter benzophenone-4 in aquatic environments: intermediates and pathways.the occurrence of benzophenone-4 (bp-4) in water environments may pose a serious public health hazard due to its potential endocrine disrupting effects. in this work, the intermediates, probable degradation pathways and toxicity changes during ozonation of bp-4 in aqueous solution were systematically investigated. results revealed that alkaline conditions favored the oxidation of bp-4. however, inorganic anions (cl(-), no3(-), so4(2-)), cations (k(+), ca(2+), mg(2+)) and humic acid had no remark ...201626855209
where does the toxicity of metal oxide nanoparticles come from: the nanoparticles, the ions, or a combination of both?the toxicity of metal oxide nanoparticles (nps) has aroused great concern over the past few years. however, there still remains the question whether the toxicity of the metal oxide nps originates from the released ions or the nps themselves. in this study, the metal ion release of cuo, fe2o3, zno, co3o4, cr2o3, and nio nps in aqueous media was investigated, and their contributions to the metal oxide nps' inhibition on the bioluminescence of photobacterium phosphoreum were studied. it was found t ...201626852208
volatile organic compounds and photobacterium phosphoreum associated with spoilage of modified-atmosphere-packaged raw pork.accumulation of volatile organic compounds was monitored in association with sensory quality, bacterial concentrations and culture-independent microbial community analyses in raw pork loin and pork collar during storage under high-oxygen modified atmosphere at +4 °c. of the 48 volatile compounds detected in the pork samples, the levels of acetoin, diacetyl and 3-methyl-1-butanol had the highest correlations with the sensory scores and bacterial concentrations. these compounds accumulated in all ...201626623935
enhanced aox accumulation and aquatic toxicity during 2,4,6-trichlorophenol degradation in a co(ii)/peroxymonosulfate/cl⁻ system.chloride ion is known to affect on degradation kinetics in different ways during ho· and so4(·-)-based advanced oxidation processes (aops). however, its effect on absorbable organic halogen (aox) evolution and acute toxicity of treated water remains unknown, despite the importance of the two parameters in evaluating the applicability of aops. in the present study, co/peroxymonosulfate (co/pms) and uv/hydrogen peroxide (uv/h2o2) treatment of 2,4,6-trichlorophenol was compared in terms of aox form ...201626613359
rapid removal of tetrabromobisphenol a by ozonation in water: oxidation products, reaction pathways and toxicity assessment.tetrabromobisphenol a (tbbpa) is one of the most widely used brominated flame retardants and has attracted more and more attention. in this work, the parent tbbpa with an initial concentration of 100 mg/l was completely removed after 6 min of ozonation at ph 8.0, and alkaline conditions favored a more rapid removal than acidic and neutral conditions. the presence of typical anions and humic acid did not significantly affect the degradation of tbbpa. the quenching test using isopropanol indicated ...201526430733
application of a new integrated sediment quality assessment method to huelva estuary and its littoral of influence (southwestern spain).a new integrated sediment quality assessment method composed of several assays (particle size profile, total metal content, protease k extraction, total organic carbon, toxicity bioassay with photobacterium phosphoreum and macrobenthic community alteration) that provides a single result, the environmental degradation index (edi), has been developed. the new method was tested on the huelva estuary (southwest of spain), a highly polluted area where metals dissolved in the water of the tinto and od ...201526260104
joint toxicity of lead, chromium, cobalt and nickel to photobacterium phosphoreum at no observed effect concentration.joint toxicity of pb2+, cr3+, co2+ and ni2+ toward photobacterium phosphoreum (ph. phosphoreum) at the no observed effect concentration (noec) was determined through a factorial experiment. a neural network model was designed according to experimental results and employed for toxicity prediction of unary, binary, ternary and quaternary combinations. the mechanism and trends of joint toxicity were interpreted by quantitative structure-activity relationship, michaelis-menten kinetic model, and con ...201526032300
[analysis of the dynamics of bioluminescence intensity of luminous bacteria photobacterium phosphoreum].this contribution presents the results of analysis of the dynamics of the bioluminescence of luminous bacteria photobacterium phosphoreum imv b-7071 under optimal conditions of their growth. a quasi-harmonic nature of the bacterial bioluminescence dynamics was detected. the observed periods of these changes have similar values compared with those in the earlier defined periods of changes in physicochemical properties of water. the relationship between biorhythms and a quasi-harmonic nature of ch ...201526016027
acute toxicity of benzophenone-type uv filters for photobacterium phosphoreum and daphnia magna: qsar analysis, interspecies relationship and integrated assessment.the hazardous potential of benzophenone (bp)-type uv filters is becoming an issue of great concern due to the wide application of these compounds in many personal care products. in the present study, the toxicities of bps to photobacterium phosphoreum and daphnia magna were determined. next, density functional theory (dft) and comparative molecular field analysis (comfa) descriptors were used to obtain more detailed insight into the structure - activity relationships and to preliminarily discuss ...201525950412
[atp pool and bioluminescence in psychrophilic bacteria photobacterium phosphoreum].bioluminescence activity and atp pool were investigated in the culture of psychrophilic bacteria photobacterium phosphoreum collected-from the exponential and stationary growth phases, as well as immobilized in polyvinyl alcohol (pva) cryogel. in liquid culture, atp pool remained at an almost a constant level throughout the luminescence cycle (over 100 h). the atp pool in the stationary-phase and pva-immobilizedl cells remained constant throughout their incubation in the medium (over 200 h) and ...201525844450
[biosensors based on the luminous bacteria photobaterium phosphoreum immobilized in polyvinyl alcohol cryogel for the monitoring of ecotoxicants].immobilization of photobacterium phosphoreum bacteria in polyvinyl alcohol cryogel was performed in order to develop biosensors used for ecotoxicant biomonitoring. the immobilization procedure, storage, and application of the immobilized cells for biomonitoring were optimized. it was shown that the immobilized cells demonstrate significantly higher stability and a longer duration of light emission than free bacteria. a discrete analysis of heavy metals and chlorophenols was conducted using the o ...201525707106
reduction in toxicity of coking wastewater to aquatic organisms by vertical tubular biological reactor.we conducted a battery of toxicity tests using photo bacterium, algae, crustacean and fish to evaluate acute toxicity profile of coking wastewater, and to evaluate the performance of a novel wastewater treatment process, vertical tubular biological reactor (vtbr), in the removal of toxicity and certain chemical pollutants. a laboratory scale vtbr system was set up to treat industrial coking wastewater, and investigated both chemicals removal efficiency and acute bio-toxicity to aquatic organisms ...201525706086
effect of fenton oxidation on biodegradability, biotoxicity and dissolved organic matter distribution of concentrated landfill leachate derived from a membrane process.the treatment of concentrated landfill leachate from membrane process is a troublesome issue due to high concentrations of biorecalcitrant pollutants. in this study, the effect of fenton process on dissolved organic matter (dom) distribution (i.e. humic acid (ha), fulvic acid (fa) and hydrophilic fraction (hyi)), chemical forms of toxic organic compounds and metals, and their biotoxicity were investigated. in the concentrated leachate, toluene, ethylbenzene and chlorobenzene predominated in the ...201525660905
predictive model for the growth of spoilage bacteria on modified atmosphere packaged atlantic salmon produced in australia.most existing models for the spoilage of modified atmosphere packed atlantic salmon are based on the growth of the spoilage organism photobacterium phosphoreum. however, there is evidence that this organism is not the specific spoilage organism on salmon produced and packaged in australia. we developed a predictive model for the growth of bacteria in australian-produced atlantic salmon stored under modified atmosphere conditions (30-98% carbon dioxide in nitrogen) at refrigeration temperatures ( ...201525583344
oxidation of tetracycline antibiotics induced by fe(iii) ions without light irradiation.the presence of fe(iii) ions was found to induce degradation of three tetracycline antibiotics (tcs), tetracycline (ttc), oxytetracycline (otc) and chlorotetracycline (ctc), in aqueous solutions without light. the presence of fe(iii) promoted the degradation of tcs in most experimental ph (5.0, 7.0 and 9.0) except at ph 9.0 for ctc. degradation rate constants of ttc, otc and ctc reached maximum ((6.2±0.5)×10(-3) h(-1), (10.6±0.1)×10(-3) h(-1) and (15.9±0.5)×10(-3) h(-1) at ph 7.0, 20 °c) when fe ...201525460769
degradation and dechlorination of pentachlorophenol by microwave-activated persulfate.the degradation performance of pentachlorophenol (pcp) by the microwave-activated persulfate (mw/ps) process was investigated in this study. the results indicated that degradation efficiency of pcp in the mw/ps process followed pseudo-first-order kinetics, and compared with conventional heating, microwave heating has a special effect of increasing the reaction rate and reducing the process time. a higher persulfate concentration and reaction temperature accelerated the pcp degradation rate. mean ...201525328098
biomarker discovery and biotherapeutics applications of photosynthetic light-harvesting and bioluminescence light-emitting chromophore-protein complexes in stem cell biology and regenerative medicine.we have since the 1970's embarked on the development of biologically derived fluorophore-protein complexes that will find applications in the communicable and non-communicable disease etiology processes and their cures. we have since then become largely successful in these endeavors along with interspersed contributions also from investigators who have generally restricted to working in confined disciplines. their encompassment with our works as this investigator has traversed his definitely cho ...201425157447
evaluation on joint toxicity of chlorinated anilines and cadmium to photobacterium phosphoreum and qsar analysis.the individual ic50 (the concentrations causing a 50% inhibition of bioluminescence after 15min exposure) of cadmium ion (cd) and nine chlorinated anilines to photobacterium phosphoreum (p. phosphoreum) were determined. in order to evaluate the combined effects of the nine chlorinated anilines and cd, the toxicities of chlorinated anilines combined with different concentrations of cd were determined, respectively. the results showed that the number of chlorinated anilines manifesting synergy wit ...201425058936
[investigation of physiological features of luminous bacteria photobacterium phosphoreum imv b-7071].bioluminescence of the strain of luminous bacteria photobacterium phosphoreum imv b-7071 has been studied. the strain was characterized by a high level of luminescence, high specific growth rate, short lag period and generation time. the authors have established the action of ph, temperature, concentration of naci on the level of bioluminescence. the maximum level of bioluminescence was observed in nacl concentration range of 2 to 8%, in the range of ph from 5.5 to 9.1 and a temperature range of ...201525007443
the joint effects on photobacterium phosphoreum of metal oxide nanoparticles and their most likely coexisting chemicals in the environment.metal oxide nanoparticles (nps) have been used increasingly and are likely to accumulate in natural water bodies, where they encounter and interact with other environmental chemicals. these interactions result in joint effects on biological systems and the environment. however, compared with the intensive research examining the toxicities of individual nps, the toxicities of np mixtures remain relatively unexplored. in this work, we studied the joint effects of nps and their most likely coexisti ...201424911590
surfactants present complex joint effects on the toxicities of metal oxide nanoparticles.the potential toxicities of nanoparticles (nps) have been intensively discussed over the past decade. in addition to their single toxicities, nps can interact with other environmental chemicals and thereby exert joint effects on biological systems and the environment. the present study investigated the combined toxicities of nps and surfactants, which are among the chemicals that most likely coexist with nps. photobacterium phosphoreum was employed as the model organism. the results indicate tha ...201424875914
genome sequence of luminous piezophile photobacterium phosphoreum ant-2200.bacteria of the genus photobacterium thrive worldwide in oceans and show substantially varied lifestyles, including free-living, commensal, pathogenic, symbiotic, and piezophilic. here, we present the genome sequence of a luminous, piezophilic photobacterium phosphoreum strain, ant-2200, isolated from a water column at 2,200 m depth in the mediterranean sea. it is the first genomic sequence of the p. phosphoreum group. an analysis of the sequence provides insight into the adaptation of bacteria ...201424744322
effect of water quality on mercury toxicity to photobacterium phosphoreum: model development and its application in natural waters.mercury (hg) compounds are widely distributed toxic environmental and industrial pollutants and they may bring danger to growth and development of aquatic organisms. the distribution of hg species in the 3 percent nacl solution was calculated using the chemical equilibrium model visual minteq, which demonstrated that hg was mainly complexed by chlorides in the ph range 5.0-9.0 and the proportions of hgcl4(2-), hgcl3(-) and hgcl2(aq) reached to 95 percent of total hg. then the effects of cations ...201424726934
balance between herbicidal activity and toxicity effect: a case study of the joint effects of triazine and phenylurea herbicides on selenastrum capricornutum and photobacterium phosphoreum.the use of herbicide mixtures has become a cost-effective strategy against the evolution of herbicide resistance to protect global food production. much research has focused on investigating either the herbicidal activities or the toxicity effects of herbicides; however, few of them have investigated both factors. this study investigates the balance between herbicidal activity for selenastrum capricornutum and toxicity effect toward photobacterium phosphoreum by determining the joint effects of ...201424681700
study on the variation rules of the joint effects for multicomponent mixtures containing cyanogenic toxicants and aldehydes based on the transition state theory.although the study of the variation rules of the joint effects for multicomponent mixtures has gained increasing attention, it still remains unclear how the variation occurs and what the relationships between the joint effects of multicomponent mixtures and their corresponding binary mixtures are. to explain how the variation occurs, this study first proposes a hypothesis on the variation rules of the joint effects using the well-known transition state theory. the hypothesis concluded that the j ...201424418495
teratogenic effects of organic extracts from the pearl river sediments on xenopus laevis embryos.toxicity of organic extracts from the pearl river sediments was investigated with xenopus laevis embryos. the effects of sediment organic extracts on the mortality, body length and malformation of x. laevis embryos were tested by the frog embryo teratogenesis assay-xenopus (fetax). the 96-h lc₅₀ values for x. laevis embryos ranged from 62 to 137 g/l (g extracted sediment per l), and the toxicity effect on body length of larvae was not significant under 20 g/l. however, the teratogenic effects pr ...201424361698
shelf life extension of whole norway lobster nephrops norvegicus using modified atmosphere packaging.once a nuisance by-catch, today the norway lobster (nephrops norvegicus) is a valuable uk fisheries commodity. unfortunately, the species is very susceptible to quality deterioration post harvest as it quickly develops black spots and also spoils rapidly due to bacterial growth. treatment with chemicals can stop the blackening and carefully monitored cold storage can result in a sensory shelf life of up to 6.5 days. the high susceptibility to spoilage greatly restricts the extent to which n. nor ...201324201017
development of a model to predict the effect of water chemistry on the acute toxicity of cadmium to photobacterium phosphoreum.cadmium (cd) compounds are widely distributed toxic environmental and industrial pollutants, and they may bring danger to growth and development of aquatic organisms. the effects of ca(2+) (as cacl2), mg(2+) (as mgso4), k(+) (as kcl), ph and complexants (edta, the commercial dom, and three homemade doms) on cd toxicity to photobacterium phosphoreum were evaluated in standardized 15 min acute toxicity tests. increases in ca(2+) concentration resulted in higher ec50 values, indicating the competit ...201324041821
quantitative structure activity relationships (qsar) for binary mixtures at non-equitoxic ratios based on toxic ratios-effects curves.the present study proposed a qsar model to predict joint effects at non-equitoxic ratios for binary mixtures containing reactive toxicants, cyanogenic compounds and aldehydes. toxicity of single and binary mixtures was measured by quantifying the decrease in light emission from the photobacterium phosphoreum for 15 min. the joint effects of binary mixtures (tu sum) can thus be obtained. the results showed that the relationships between toxic ratios of the individual chemicals and their joint eff ...201323930105
effects of hydrostatic pressure on growth and luminescence of a moderately-piezophilic luminous bacteria photobacterium phosphoreum ant-2200.bacterial bioluminescence is commonly found in the deep sea and depends on environmental conditions. photobacterium phosphoreum ant-2200 has been isolated from the nw mediterranean sea at 2200-m depth (in situ temperature of 13°c) close to the antares neutrino telescope. the effects of hydrostatic pressure on its growth and luminescence have been investigated under controlled laboratory conditions, using a specifically developed high-pressure bioluminescence system. the growth rate and the maxim ...201323818946
the underlying toxicological mechanism of chemical mixtures: a case study on mixture toxicity of cyanogenic toxicants and aldehydes to photobacterium phosphoreum.intracellular chemical reaction of chemical mixtures is one of the main reasons that cause synergistic or antagonistic effects. however, it still remains unclear what the influencing factors on the intracellular chemical reaction are, and how they influence on the toxicological mechanism of chemical mixtures. to reveal this underlying toxicological mechanism of chemical mixtures, a case study on mixture toxicity of cyanogenic toxicants and aldehydes to photobacterium phosphoreum was employed, an ...201323811331
toxicity testing with luminescent bacteria--characterization of an automated method for the combined assessment of acute and chronic effects.the luminescent bacteria test according to en iso 11348 is frequently applied in (eco) toxicity testing and is applicable for a huge variety of environmental and industrial samples. a big disadvantage of this method is the very short exposure time, which is expressed in a low sensitivity in regard to substances with a delayed effect. chronic effects, i.e. interference with cell growth, cannot be assessed with this conventional standard method. the goal of this research was to develop an automate ...201323806483
the toxicity of cadmium to three aquatic organisms (photobacterium phosphoreum, daphnia magna and carassius auratus) under different ph levels.this study investigated the effect of ph on cadmium toxicity to three aquatic organisms: photobacterium phosphoreum, daphnia magna and carassius auratus. the acute toxicity of cd(2+) to p. phosphoreum and d. magna at five ph values (5.0, 6.0, 7.0, 8.0, and 9.0) was assessed by calculating ec50 values. we determined that cd(2+) was least toxic under acidic conditions, and d. magna was more sensitive to the toxicity of cd than p. phosphoreum. to evaluate cd(2+)-induced hepatic oxidative stress in ...201323769002
ozonation of trimethoprim in aqueous solution: identification of reaction products and their toxicity.this work aimed to better understand the ozonation process of a typical antibiotic pharmaceutical, trimethoprim in aqueous solution. the parent compound was almost completely degraded with ozone dose up to 3.5 mg/l with no mineralization. twenty one degradation products were identified using an electrospray quadrupole time-of-flight mass spectrometer. several ozonation pathways were proposed including hydroxylation, demethylation, carbonylation, deamination and methylene group cleavage. two spec ...201323541309
effect of tritium on luminous marine bacteria and enzyme reactions.the paper studies chronic effect of tritiated water, hto, (0.0002-200 mbq/l) on bioluminescent assay systems: marine bacteria photobacterium phosphoreum (intact and lyophilized) and coupled enzyme reactions. bioluminescence intensity serves as a marker of physiological activity. linear dependencies of bioluminescent intensity on exposure time or radioactivity were not revealed. three successive stages in bacterial bioluminescence response to hto were found: (1) absence of the effect, (2) activat ...201323410594
[acute toxicity of antibiotics and anaerobic digestion intermediates in pharmaceutical wastewaters].in order to determine the toxicity of antibiotics and anaerobic digestion intermediates on anaerobic treatment of pharmaceutical wastewaters containing antibiotics, the single and joint toxicities of some antibiotics and intermediates to photobacterium phosphoreum were tested by using the 15-min half inhibitory concentration (15 min-ic50) at ph = 7.00 +/- 0.05. the results showed that the 15 min-ic50 of ethanol, acetate, propionate and butyrate were 19.40, 20.71, 10.47 and 12.17 g x l(-1), respe ...201223379166
bioluminescence as a tool for studying detoxification processes in metal salt solutions involving humic substances.the paper considers effects of humic substances (hs), as natural attenuators of toxicity, on solutions of model inorganic pollutants, metal salts - pb(no(3))(2), сосl(2), cuso(4), eu(no(3))(3), сrсl(3), and k(3)[fe(сn)(6)]. luminous bacteria photobacterium phosphoreum and bioluminescent system of coupled enzymatic reactions were used as bioassays to monitor toxicity of salt solutions. the ability of hs to decrease or increase toxicity was demonstrated. detoxifying concentrations of hs were deter ...201223123596
evaluation of joint toxicity of nitroaromatic compounds and copper to photobacterium phosphoreum and qsar analysis.the individual toxicities of cu and 11 nitroaromatic compounds to photobacterium phosphoreum were determined. the toxicity was expressed as the concentrations causing a 50% inhibition of bioluminescence after 15 min exposure (ic(50)). to evaluate the joint effect between the metal ion and the 11 nitroaromatic compounds, the joint toxicity of cu and 11 nitroaromatic compounds were measured at different cu concentrations (0.2ic(50), 0.5ic(50) and 0.8ic(50)), respectively. the result shows that the ...201223089062
exploring qstr analysis of the toxicity of phenols and thiophenols using machine learning methods.there is an increasing need for the rapid safety assessment of chemicals by both industries and regulatory agencies throughout the world. in silico techniques are practical alternatives in the environmental hazard assessment. in this background, quantitative structure-toxicity relationship (qstr) analysis has been performed on toxicity of phenols and thiophenols to photobacterium phosphoreum. the techniques of classification and regression trees (cart) and least squares support vector regression ...201223068157
loss-of-function mutation in bi-functional marine bacterial sialyltransferase.an α2,3-sialyltransferase produced by photobacterium phosphoreum jt-ish-467 is a bi-functional enzyme showing both α2,3-sialyltransferase and α2,3-linkage specific sialidase activity. to date, the crystal structures of several sialyltransferases have been solved, but the roles of amino acid residues around the catalytic site have not been completely clarified. hence we performed a mutational study using α2,3-sialyltransferase cloned from p. phosphoreum jt-ish-467 as a model enzyme to study the r ...201222972324
effects of americium-241 and humic substances on photobacterium phosphoreum: bioluminescence and diffuse reflectance ftir spectroscopic studies.the integral bioluminescence (bl) intensity of live photobacterium phosphoreum cells (strain 1883 ibso), sampled at the stationary growth stage (20 h), was monitored for further 300 h in the absence (control) and presence of (241)am (an α-emitting radionuclide of a high specific activity) in the growth medium. the activity concentration of (241)am was 2 kbq l(-1); [(241)am]=6.5×10(-11) m. parallel experiments were also performed with water-soluble humic substances (hs, 2.5 mg l(-1); containing o ...201322795580
model of hormesis and its toxicity mechanism based on quorum sensing: a case study on the toxicity of sulfonamides to photobacterium phosphoreum.during the past two decades, the phenomenon of hormesis has gained increasing recognition in environmental and toxicological communities. however, the mechanistic understanding of hormesis, to date, is extremely limited. herein is proposed a novel parametric model with a mechanistic basis and two model-based parameters for hormesis that was successfully applied to the hormetic dose-response observed in the chronic toxicity of sulfonamides on photobacterium phosphoreum. on the basis of the method ...201222715968
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