| [clinical aspects of pseudotuberculosis]. | | 1975 | 1103463 |
| [successful streptomycin therapy of yersinia pseudotuberculosis and acinobacter iwoffii infection]. | | 1975 | 1105300 |
| [interaction of the causative agent of pseudotuberculosis with the peritoneal macrophages from an immune and a nonimmune organism]. | the role of phagocytosis in experimental pseudotuberculosis was demonstrated in vivo and in vitro. virulent y. pseudotuberculosis strain caused the death of the majority of the cells, whereas the weakly-virulent ones--degenerative changes and the death of but of few of them. the capacity of the causative agents of pseudotuberculosis to survive and to reproduce within the cells could be regarded as one of the significant factors of virulence of these microbes. the maximal immunizing effect was ob ... | 1975 | 1108525 |
| drug screening by enzymatic immunoassay with the centrifugal analyzer. | | 1975 | 1112024 |
| age of cattle as related to resistance to infection with mycobacterium paratuberculosis. | two 1-month-old calves, four 9-month old calves, and 4 adult (5 to 11 years old) cattle were exposed to mycobacterium paratuberculosis. at 150 days after exposure, all cattle were killed and necropsied, and at that time, tissues of the 1-month-old calves had more bacilli and lesions than did tissues of the 9-month-old calves or the adult cattle. all cattle responded to immunologic tests during the experiment. | 1975 | 1115424 |
| proceedings: cell-wall composition of aerobic cutaneous "diphtherios" organisms. | | 1975 | 1142405 |
| effects of sodium acetylsalicylate on body temperature of monkeys under heat exposure. | exposure of taiwan monkeys to 38 degrees c ambient temperature caused: a) raised body temperature, b) restlessness and struggling, c) increased evaporative heat loss and d) increase in tail skin temperature. after the administration of sodium acetylsalicylate, 100 to 250 mg/kg intraperitoneally or 4 to 15 mg intracerebroventricularly (third ventricle) the same heat load caused: a) less increase in body temperature, b) no restlessness or struggling, c) an initially higher rate of evaporative heat ... | 1975 | 1151748 |
| proceedings: effects of histamine, 2-methylhistamine and 4-methylhistamine on blood pressure and vascular resistance in the cat. | | 1975 | 1151865 |
| comparative morphology of genital cones of genus ancylostoma dubini, 1843. | male worms of ancylostoma braziliense, a. ceylanicum, a. kusimaense, a. malayanum, a. duodenale, a. caninum, a. tubaeforme, agriostomum vryburgi and cyclodontostomum purvisi have the external appendages beside the anogenital aperture. these anogenital structures are morphologically similar and are assumed to be homologous among the species with three pairs of teeth or more. in hookworms with two pairs of teeth, the anogenital structures are complex. anogenital features can be used as a taxonomic ... | 1975 | 1166351 |
| estimation of plasma testosterone without extraction and chromatography. | a new radioimmunoassay for plasma testosterone in human blood plasma is described. the assay is performed directly upon the plasma without extraction and purification of the testosterone. antibodies to testosterone-7alpha-carboxyethyl thioether-bovine serum albumin are used and data concerning the specificity of these antibodies are given. | 1975 | 1168552 |
| prevalence of diabetes mellitus among the ethnic groups in singapore. | during a routine medical examination of 5280 government employees (2736 males, 2544 females; age range 17 to 66 years; 3386 chinese, 1252 malays, 508 indians and 134 other ethnic groups) diabetes was found in 31 (0.59%). the prevalence was higher in males (0.95%) than in females (0.20%). the highest prevalence was in the age-group 50 to 59 years (5.4%); at ages 30 to 66 years, the overall prevalence was 2.18%. the prevalence of diabetes is higher in indians (2.76%) than in chinese (0.30%) and ma ... | 1975 | 1169832 |
| structure of the somatic appendages of neurons of locus coeruleus in cat. | | 1975 | 1175016 |
| time-dependent changes in commissural field potentials in the dentate gyrus following lesions of the entorhinal cortex in adult rats. | previous neuroanatomical work has shown that lesions of the entorhinal cortex in adult rats cause the commissural projections to spread from their normally restricted locus in the inner molecular layer approximately 40-50 mum into the outer molecular layer (that is, into the zone deafferented by the lesion). in the present study we measured the effects of the entorhinal lesion on the distribution of short-latency potentials elicited by commissural stimulation in the molecular layer. studies with ... | 1975 | 1175043 |
| neonatal androgenic stimulation and adult sexual behavior in male and female golden hamsters. | neonatally and adult castrated male hamsters as well as neonatally androgenized and nonandrogenized female hamsters were tested for both mounting and lordosis behaviors during treatment with either testosterone or ovarian hormones. neonatal androgenization facilitated mounting behavior in adult animals administered either testosterone or ovarian hormones and suppressed lordosis behavior in adult ovarian-hormone-treated animals. early androgen effects on the display of lordosis behavior during ad ... | 1975 | 1194451 |
| the functional consequences of colectomy. | the colon plays a decisive role in salt and water conservation in the intact human, normally removing from the terminal intestine approximately one liter of isotonic fluid that escapes small bowel absorption. the primary purpose of this colon function is probably to prevent extracellular fluid volume depletion and only incidentally to produce a normal solid stool. the patient with an ileostomy can partly adapt to replace the absorptive capacity lost after colectomy but is still vulnerable if sal ... | 1975 | 1200267 |
| ultrastructural studies of the bone marrow in sickle cell anaemia. ii. the morphology of erythropoietic cells and their response to deoxygenation in vitro. | electron microscopic studies of bone marrow aspirates obtained from patients with homozygous sickle cell anaemia (hbss) were fixed immediately without attempts to deoxygenate the samples. erythroblasts and normoblasts in these preparations were devoid of haemoglobin polymers or other indications of sickling. furthermore, the nucleated erythroid cells from sickle-cell patients presented an ultrastructural morphology indistinguishable from that of identically-processed erythroid cells in marrow sa ... | 1975 | 1201249 |
| [study of the epidemiology of far eastern scarlatina-like fever in the maritime territory. i. contamination of vegetables, root crops and pickled foods with the pseudotuberculosis microbe (yersinia pseudotuberculosis) in foci of the scarlatina-like fever]. | there was revealed high incidence of contamination with yersinia pseudotuberculosis of vegetables, roots and pickled products (less frequently) in the vegetable store-houses of the majority of the far eastern scarlatina-like fever foci in primorie. contamination of the mentioned food products increased by the spring-beginning of summer seasons; vegetables and roots which are often used raw--onion, carrots, cabbage--displayed the greastest contamination. | 1975 | 1210894 |
| [changes of the inner ear in experimental stapedectomy]. | | 1975 | 1230953 |
| [induction of interphase chromosome condensation in cultured human lymphocytes]. | | 1975 | 1231100 |
| [electron microscopic studies of epinephrine choroiditis in rabbits. 3. scanning electron microscopic observations of the retinal pigment epithelial cells in the early stage (author's transl)]. | | 1975 | 1239915 |
| temperature-dependent cultural and biochemical characteristics of rhamnose-positive yersinia enterocolitica. | clinical isolates of rhamnose-positive yersinia enterocolitica (y.e.rh+) were compared with typical rhamnose-negative y. enterocolitica (y.e.rh-) and with yersinia pseudotuberculosis. the y.e.rh+ differed from the y.e.rh- and y. pseudotuberculosis in their ability to ferment raffinose and lactose, utilize citrate and in their inability to grow on hektoen enteric agar at 22 or 37 c, on salmonella-shigella agar at 37 c, and scant on xylose-lysine-deoxycholate agar at 37 c. an extensive temperature ... | 1976 | 1254709 |
| [yersinia enterocolitica and yersinia pseudotuberculosis in the pathogenesis of abdominal pain in children]. | | 1976 | 1256919 |
| the effect of subdermal testosterone filled polydimethylsiloxane implants on spermatogenesis in rhesus monkeys (macaca mulata). | previously it was reported that testosterone-filled polydimethyl silicone (pds) implants induced azoospermia in male rabbits without chronically altering plasma testosterone concentration or the weight or secretory activity of accessory sex glands. assayable serum luteinizing hormone (lh) and testicular testosterone secretion were completely suppressed. testosterone emanating from the pds capsule maintained libido but failed to support spermatogeneis in the rabbits. to test results in subhuman ... | 1976 | 1261261 |
| attenuated, streptomycin-dependent salmonella typhi oral vaccine: potential deleterious effects of lyophilization. | four studies were done with streptomycin-dependent salmonella typhi as an oral, attenuated vaccine. studies 1 and 3 employed freshly harvested vaccine, whereas studies 2 and 4 involved lyophilized vaccine. five to eight doses (3 x 10(10)-10(11) organisms/dose) were given; oral streptomycin (1.0 g) was administered concomitantly in studies 2 and 3, with only two of the doses of vaccine in study 1, and was not given in study 4. no adverse reactions were encountered in 179 vaccinated men, and 94% o ... | 1976 | 1262709 |
| the toxic effects of dieldrin in rats: a reevalution of data obtained in a two-year feeding study. | | 1976 | 1273845 |
| lipopolysaccharide from yersinia pseudotuberculosis, type 1 b. a structural study of o-specific chains. | | 1976 | 1278178 |
| immunocytochemistry of oculomotor afferents in the squirrel monkey (saimiri sciureus). | attempts were made to co-define afferents of the oculomotor nuclear complex (omc) and their putative neurotransmitters in the squirrel monkey. wheat germ agglutinin conjugated to horseradish peroxidase (wga-hrp) and wheat germ agglutinin conjugated to enzymatically inactive hrp and coupled to colloidal gold (wgaapohrp-au) were used as retrograde tracers in combination with immunocytochemical methods. primarily unilateral injections were made into portions of the omc. stabilized tetramethylbenzid ... | 1992 | 1280294 |
| the yersinia tyrosine phosphatase: specificity of a bacterial virulence determinant for phosphoproteins in the j774a.1 macrophage. | yoph is a plasmid-encoded protein tyrosine phosphatase (ptpase) secreted by pathogenic yersinia. although the enzyme likely acts to dephosphorylate eukaryotic proteins during yersinia infection of the mammalian host, the targets of yoph have not been identified. we infected the murine macrophage-like cell line j774a.1 with yersinia pseudotuberculosis and investigated the specificity of yoph and yophc403a, a catalytically inactive mutant derivative, for eukaryotic phosphoproteins. upon infection, ... | 1992 | 1281213 |
| regeneration of an adult peripheral nerve preparation in culture. | the methods used to maintain the vagus nerve from the adult rat in culture and how regeneration is studied in this preparation are described. a hypothesis is presented on the triggering of the cell body reaction. it is suggested that this reaction is initiated by proteins synthesized in nonneuronal cells at the site of a nerve lesion. these proteins, referred to as regenerins, reach the nerve cell body by retrograde axonal transport, where they initiate the regeneration process. | 1992 | 1282333 |
| child health surveillance. surveillance of the child under 5. | 1. general health checks should be made at the following times as outlined in these guidelines: initial neonatal assessment 7-10 day check, 6-week check, 7-9 months: general examination with particular attention to hearing and vision, 18-24 months: check with special attention to gait, speech and understanding, 36-42 months: general examination and developmental assessment, 2. parental concern over a child's special senses should be carefully followed up and investigated. 3. immunization schedul ... | 1992 | 1285363 |
| changes in burying behavior during the estrous cycle: effect of estrogen and progesterone. | this study demonstrates changes in cumulative burying behavior during various phases of the rat estrous cycle. low levels of burying behavior were observed during late proestrus. similar burying behavior levels among females tested in early proestrus, metestrus or diestrus and ovariectomized females were found. in ovariectomized females, treatment with estrogen (e) produced a slight, non-significant reduction in burying behavior. in contrast, progesterone (p) treatment resulted in a dose-depende ... | 1992 | 1287686 |
| [stable and efficient expression of human interleukin-6 cdna in mammalian cells after gene transfer]. | following reverse transcription and pcr amplification, the human interleukin-6 (il-6) cdna was cloned from total rna of activated human tonsillar mononuclear cells. southern blot showed the presence of specific band, and its dna sequence demonstrated that the fragment was 650 bp in length, spanning the complete coding region and part of 5' and 3'-untranslated regions. the human il-6 sequence seemed to be well conserved. one nucleotide change at 429 position was observed, but this change did not ... | 1992 | 1291290 |
| a patch-clamp study of the ca2+ mobilization from internal stores in bovine aortic endothelial cells. i. effects of caffeine on intracellular ca2+ stores. | the effects of agents known to interfere with ca2+ release processes of endoplasmic reticulum were investigated in bradykinin (bk)-stimulated bovine aortic endothelial cells (bae cells), via the activation of ca(2+)-activated potassium channels [k(ca2+) channels]. in cell-attached patch experiments, the external application of caffeine (1 mm) caused a brief activation of k(ca2+) channels in ca(2+)-free and ca(2+)-containing external solutions. the application of bk (10 nm) during cell stimulatio ... | 1992 | 1291681 |
| [detection of mycobacteria by dna amplification]. | polymerase chain reaction (pcr) was used to detect and to identify mycobacterium species. in this study, 13 out of 14 mycobacterium species were detected by using six pairs of oligonucleotide primers. the pcr product was detected by non-isotopic southern blot hybridization even when as little as 10 fg of purified m. tuberculosis dna was used. and 8 mycobacterial species were identified by pcr-restriction fragment length polymorphism (rflp) using two kinds of endonuclease. | 1992 | 1294656 |
| [dna probe for detecting yersinia pestis and serovariant i of yersinia pseudotuberculosis by detecting specific dna repeating sequences]. | in order to construct a dna probe for the plague pathogen detection, we have obtained the recombinant plasmid prd100 carrying an ecori-flanked 140 bp fragment from the genetically silent region of yersinia pestis species-specific plasmid pyp1. when used as a dna probe for hybridization of dna from various strains of 25 bacterial species, this dna fragment was shown to have the complementary sequences in all investigated yersinia pestis strains (200), including the plasmid pyp1 lacking ones, and ... | 1992 | 1298882 |
| [studies of the lymphatic system in mice infected with trichinella pseudospiralis as well as infected and stimulated by phytohaemagglutin (pha-p)]. | mice cfw were infected per os with trichinella pseudospiralis (150 larvae/mice). the mice were divided in two groups: nonstimulated and stimulated intraperitoneally with pha-p at dose 10 mg/kg body weight. the animals were killed 7, 14, 21, 28, 42, 56, 70 and 150 day post infection (d.p.i.). spleen et mesenteric lymph nodes were examined histopathologically (hematoxylin-eosin) and histochemically (nonspecific esterase reaction) for differentiation of t lymphocytes. the obtained results reveal th ... | 1992 | 1299055 |
| [the interleukin-1 content of the blood in patients with a pseudotuberculosis infection and opisthorchiasis]. | interleukin-1 levels were measured according to s. b. mizel in 25 patients with pseudotuberculosis and concomitant chronic opisthorchiasis and in 25 ones with pseudotuberculosis alone over the course of the disease and in 3 months after it. the controls were patients with chronic opisthorchiasis and normal subjects. hyperproduction of interleukin-1 by peripheral blood monocytes was found in chronic opisthorchiasis patients and in pseudotuberculosis patients with concomitant opisthorchiasis over ... | 1992 | 1299750 |
| serological diagnosis in human yersinia infections. | serological examinations revealed y. enterocolitica 0:3 specific antibodies in 34% of 80 children affected by a yersiniosis epidemic at titres of 1/40-1/640 and in all sporadic yersinia enteritis cases. similarly, agglutinins were identified for y. enterocolitica 0:3 and 0:9 in 3.4% of 430 appendectomized children hospitalized with painful syndrome in the right iliac fossa. in 10% of 101 patients hospitalized with arthritis and reiter's syndrome antibodies to y. enterocolitica 0:3 (8 cases) and ... | 1992 | 1304827 |
| [the hypolipidemic action of water-soluble enterosorbents of cholesterol and bile acids in an experiment]. | experiments on rats, guinea pigs and rabbits with experimental dyslipoproteinemia have revealed that the new water-soluble high-molecular weight polymers containing digitonin (ed) or a quaternary nitrogen atom (ea) have a more marked hypolipidemic action than cholestyramine and normalize lipoprotein metabolism. the digitonin-containing enterosorbent has been shown to decrease the absorption of 4-14c-cholesterol, while the nitrogen-containing enterosorbent diminishes the absorption of 3h-cholic a ... | 1992 | 1305451 |
| [a trial application of the latex test for evaluation of antibody level in rabbit immune sera]. | a latex test was elaborated which served for evaluation of quality of rabbit immune sera for antigen 0 of selected gram-negative bacteria. sensitivity and specificity of this test in comparison with passive hemagglutination and immunoenzymatic dot-elisa reactions was evaluated. these studies were performed on immune sera for antigen o of salmonella groups b, c1, c2, d and e, yersinia pseudotuberculosis and in antigen preparations for above listed microorganisms both in homologous and heterologou ... | 1992 | 1305916 |
| expression and release of neuroregulators during development: monoamines and neuropeptides. | the turnover of catecholamines (ca) was determined in the adrenal medulla and brain of rat fetuses and pups. in general we found a considerable increase soon after birth. the expression of mrna for ca-synthesizing enzymes was also considerably enhanced in the adrenals shortly after birth. furthermore, we demonstrated increased expression of neuropeptides after birth, increased synthesis of mrna encoding for neuropeptide y in the adrenals 24 h after birth; and considerable activation of the subst ... | 1992 | 1306802 |
| [the dynamic indices of human thermal status during overheating exposures]. | the thermal state of 7 naked test subjects on a 25 min of work (325 j/s) at 40 degrees c and 85% humidity of the ambient air has exceeded the acceptable limits. the thermal protecting garment having high thermal resistance indices was tested near a fire source. the subjects performed the 254 j/s and 403 j/s work so long as they were able to do it. time of work was, on average, 17 and 12 min, respectively. by the end of work, thermal state of the subjects approached to a "limiting" value: heart r ... | 1992 | 1307041 |
| epidemiology of yersinia pseudotuberculosis and y. enterocolitica infections in sheep in australia. | infections with yersinia pseudotuberculosis serotype iii and y. enterocolitica serotype o2,3 were found to be common in australian sheep flocks. transmission of y. pseudotuberculosis occurred in late winter and early spring, while y. enterocolitica transmission occurred from midwinter to early summer. excretion of y. pseudotuberculosis was limited to the winter and spring period and was particularly common in 1- and 2-year-old sheep. infection persisted for up to 14 weeks. y. pseudotuberculosis ... | 1992 | 1313049 |
| on employing leishmania promastigotes for systemic lupus erythematosus diagnosis. | based on having definite double stranded dna determinants in the kinetoplast, it was attempted to use leishmania promastigotes as an ifat substrate for the detection of serum anti ds-dna autoantibodies which are the prominent diagnostic marker of sle. serological inspection of different leishmania preparations revealed that leishmania is not analogous to crithidia lucilia as an appropriate ds-dna substrate. it seems likely that the species-specific molecular characters of the kinetoplast-dna are ... | 1992 | 1315832 |
| kappa-opioid receptor stimulation abolishes mu- but not delta-mediated inhibitory control of spinal met-enkephalin release. | the possible opioid control through delta, mu and kappa receptors of the spinal release of met-enkephalin-like material (melm) was investigated in halothane-anaesthetized rats. the intrathecal perfusion of the delta agonist dtlet (10 microm) or the mu agonist dago (10 microm) resulted in a marked inhibition of melm release, which could be prevented by the selective antagonists naltrindole and naloxone, respectively. although the kappa agonist u 50488 h (10 microm) was inactive per se, it complet ... | 1992 | 1317026 |
| the pathogenicity of drug-resistant variants of herpes simplex virus. | | 1992 | 1317602 |
| the clinical significance of in vitro cytomegalovirus susceptibility to antiviral drugs. | | 1992 | 1317603 |
| new monoclonal antibodies for the detection of immediate early antigens of cytomegalovirus. | two new monoclonal antibodies, cie-1 and cie-2, were developed for the rapid detection of human cytomegalovirus (hcmv) infection. they were found to be reactive with immediate early protein of hcmv in the nuclei of infected fibroblasts, as early as 3 hours post-infection. by radioimmunoprecipitation, cie-1 was found to react with a protein with an apparent molecular weight of 70,000, whereas cie-2 precipitated 2 proteins of 70,000 and 72,000 daltons, respectively. both monoclonal antibodies reco ... | 1992 | 1319172 |
| effect of inhibitors of hela cell structures and functions on escherichia coli hb101 (pri203) entry process. | we investigated the effect of some eucaryotic cytoplasmic structure and function inhibitors on the entry into hela cells of the escherichia coli hb101 k12 strain, harbouring the recombinant plasmid pri203, in which is cloned a 3.2 kb chromosomal fragment of yersinia pseudotuberculosis. substances impairing microfilament structures and functions (cytochalasin b and trifluoroperazine) significantly reduced invasion ability whereas microtubule organization inhibitors (colchicine and vinblastine) we ... | 1992 | 1343941 |
| detection of human papillomavirus types 16 and 18 in the exfoliated cervical cells using the polymerase chain reaction. | we applied the polymerase chain reaction (pcr) to detect hpv 16 and 18 in cytological samples obtained from the uterine cervices of japanese women. hpv infection was detected in 17 (25%) of 67 with cin and 11 (37%) of 30 with cervical carcinoma. it is notable that 11 (16%) of 69 women with normal cervices were infected with either hpv 16 or 18. the polymerase chain reaction is sensitive and useful for epidemiological studies. | 1992 | 1346595 |
| nh2-terminal globular domain of human platelet glycoprotein ib alpha has a methionine 145/threonine145 amino acid polymorphism, which is associated with the hpa-2 (ko) alloantigens. | the glycoprotein (gp) ib/ix complex, a prominent platelet gp complex, is the primary receptor for vwf. previously, we have established that an antigenic polymorphism of platelets, the hpa-2 or ko alloantigen system, is located on the 45-kd amino-terminal globular domain of gpib alpha. with the polymerase chain reaction, we have amplified two segments of the gpib alpha gene coding for the first 382 amino acids of two hpa-2a and two hpa-2b homozygous individuals. nucleotide sequence analysis revea ... | 1992 | 1346615 |
| bismuth subsalicylate in the treatment of h2 blocker resistant duodenal ulcers: role of helicobacter pylori. | fifty nine patients with helicobacter pylori positive duodenal ulcers that failed to heal after a six week course of treatment with h2 blockers were randomly assigned to one of the following three regimens: (i) bismuth subsalicylate, 600 mg three times daily (n = 19), (ii) ranitidine, 300 mg at night (n = 20), (iii) bismuth subsalicylate plus ranitidine (n = 20). cumulative ulcer healing rates after four and eight weeks respectively were as follows: bismuth subsalicylate 74% (14/19) and 95% (18/ ... | 1992 | 1347278 |
| [h2-receptor antagonist-refractory ulcer--its pathophysiology and role of proton-pump inhibitors]. | among h2-receptor antagonist (h2ra)-refractory ulcers, non-responders that did not heal after 5 months therapy had high intraluminal ph in the basal condition and high sensitivity to inhibition of acid secretion by h2ra but possessed gastric mucosa to generate less prostaglandins. combination therapy of pge1-analogue with h2ra healed these ulcers by 60%. proton-pump inhibitor (ppi) exerted a complete inhibition of acid secretion in these patients and the rate of healing was 88%. helicobacter pyl ... | 1992 | 1347325 |
| characterization of serpulina (treponema) hyodysenteriae and related intestinal spirochetes by ribosomal rna gene restriction patterns. | serpulina (treponema) hyodysenteriae strain a-1 partially purified rrna, labelled with photobiotin, was used as a non-radioactive probe to identify the rrna gene restriction patterns of s. hyodysenteriae strains and other spirochetes. sau3a restriction enzyme digests resulted in similar rrna gene restriction patterns in s. hyodysenteriae strains from five different countries. some s. hyodysenteriae strains could be differentiated by variations in their rrna gene restriction patterns after cleava ... | 1992 | 1354196 |
| stereoselective effects of central alpha 2-adrenergic agonist medetomidine on in vivo catechol activity in the rat rostral ventrolateral medulla (rvlm). | the stereoselective central effects of a novel, highly potent and selective alpha 2-agonist medetomidine on adrenergic neuronal activity, reflected by changes in catechol oxidation current, in the rostral ventrolateral medulla of the halothane-anesthetized rat were examined using in vivo differential normal pulse voltammetry. dexmedetomidine, the active isomer, significantly decreased catechol oxidation current to 33.4 +/- 4.5% of baseline when given centrally (1 microgram, i.c.v.) and to 10.3 + ... | 1992 | 1360311 |
| effects of l-156,602, a c5a receptor antagonist, on mouse experimental models of inflammation. | l-156,602, a c5a receptor antagonist, was found as an immunosuppressant with preferential effects on delayed-type hypersensitivity (dth) in our screening program and it was shown that l-156,602 suppressed the efferent phase of dth. here, we tested its effects on experimental models of inflammation induced in mice. l-156,602 did not suppress serotonin- and carrageenan- induced inflammation while it completely suppressed concanavalin a-induced inflammation 4 h after elicitation. the inflammation a ... | 1992 | 1369097 |
| characterization of zymomonas mobilis alkaline phosphatase activity in escherichia coli. | zymomonas mobilis phoa gene encoding alkaline phosphatase was expressed in escherichia coli cc118 carrying the recombinant plasmid pzap1. the ph optimum for this enzyme was 9.0 and showed a peak activity at 42 degrees c. this enzyme required zn2+ for its catalytic activity; however, mg2+ or ca2+ significantly affected the activity. this enzyme was found to be ethanolabile, and ethanol inhibition was reversed by addition of zn2+. kinetics of z. mobilis alkaline phosphatase production in e. coli c ... | 1992 | 1369189 |
| reduction of langerhans cells in smokeless tobacco-associated oral mucosal lesions. | localized absence of epithelial langerhans cells (lc) has been shown to affect systemic immune responses, allow microbial colonization and play a possible role in carcinogenesis. because use of smokeless tobacco is associated with abnormal oral mucosal changes and development of carcinoma, we examined lesion and control specimens from 17 current users of smokeless tobacco to determine whether lesions showed changes in lc number or antigen expression. we identified lc by immunohistochemistry with ... | 1992 | 1374796 |
| synthesis of 3,6-dideoxy-4-c-(4(1)-hydroxyethyl)hexopyranoses (yersinioses) from 1,6-anhydro-beta-d-glucopyranose. | | 1992 | 1379516 |
| a novel temporal expression pattern of three c/ebp family members in differentiating myelomonocytic cells. | members of the ccaat/enhancer binding protein (c/ebp) family have been shown to regulate the terminal differentiation of adipocytes and hepatocytes. in these cell lineages, high levels of c/ebp alpha are found only in mature, nondividing cells. using western blotting and immunohistochemical staining, we have determined the temporal order of expression for c/ebp alpha, c/ebp beta, and c/ebp delta in differentiating myelomonocytic marrow cells. these studies show a unique temporal pattern of c/ebp ... | 1992 | 1391942 |
| invasin expression in yersinia pseudotuberculosis. | a 3.2-kb region on the chromosome of yersinia pseudotuberculosis, called inv, encodes invasin, a 103-kda protein of the bacterial outer membrane. invasin mediates bacterial entry into cultured animal cells. six y. pseudotuberculosis strains isolated from animal or human infections were analyzed for the presence of inv-related sequences with a radiolabeled inv clone, pri203. we found that inv-specific sequences were present in all strains studied. strains cured of virulence plasmid pyv were studi ... | 1992 | 1398952 |
| detection and identification of yersinia pseudotuberculosis and pathogenic yersinia enterocolitica by an improved polymerase chain reaction method. | we developed a polymerase chain reaction method in order to detect and identify both yersinia pseudotuberculosis and pathogenic yersinia enterocolitica. polymerase chain reaction was performed by using a mixture of primers against the inv gene from y. pseudotuberculosis and the ail gene from pathogenic y. enterocolitica. further addition of primers against the plasmid-coded virf gene from y. enterocolitica made it possible to detect a virulence-associated gene of both species at the same time. t ... | 1992 | 1401022 |
| [detection and identification method of pathogenic yersinia by pcr technique]. | | 1992 | 1404926 |
| travelers' diarrhea. prevention and treatment. | travelers' diarrhea affects almost half of all travelers from developed to developing nations. its occurrence frequently alters planned activities. enterotoxigenic escherichia coli and other bacterial pathogens endemic to the area visited account for the majority of pathogens isolated. newer research technologies are assisting in defining virulence properties and epidemiologic characteristics. prudence exercised during travel remains the preferred mode of prevention, reserving antimicrobial prop ... | 1992 | 1405823 |
| extracellular localization of a nonfimbrial hemagglutinin of yersinia pseudotuberculosis. | a nonfimbrial hemagglutinin (ha) of yersinia pseudotuberculosis was observed by immunoelectron microscopy using monospecific ha antiserum and protein a-gold conjugate. the ha, an amorphous but morphologically identifiable entity, was located in a region distal to or detached from the outer edge of bacteria. | 1992 | 1406379 |
| [acute destructive appendicitis associated with various infectious diseases]. | | 1992 | 1408575 |
| linoleate-dependent co-oxygenation of benzo(a)pyrene and benzo(a)pyrene-7,8-dihydrodiol by rat cytosolic lipoxygenase. | 1. co-oxygenation of 14c-labelled benzo(a)pyrene and benzo(a)pyrene-7,8-dihydrodiol was studied in rat lung cytosol, using linoleic acid as a co-substrate. covalently bound and soluble metabolites were quantified by radiometry and h.p.l.c., respectively. 2. the co-oxygenation resulted in the production of reactive metabolites capable of protein binding as well as a series of soluble derivatives. 3. co-oxygenation of benzo(a)pyrene yielded primarily a significant amount of benzo(a)pyrene-6,12-dio ... | 1992 | 1413876 |
| [the temperature-induced early and late development of resistance to the bactericidal action of blood serum in yersinia pseudotuberculosis]. | y. pseudotuberculosis strains were grown at 6 degrees-8 degrees c and then incubated at 37 degrees c. 3-6 hours later serum resistance appeared in the strains having plasmid virulence and producing outer membrane polypeptide with a molecular weight of 120 kd, known as p1. 10-12 hours later serum resistance appeared in the strain having the virulence plasmid, but not producing p1, as well as in strains in which the plasmid was eliminated. | 1992 | 1414100 |
| effects of morphine on acquisition and maintenance of ethanol and water intake patterns in rats. | two experiments were conducted to assess the effects of chronic subcutaneous injections of morphine (1.0 mg/kg) or saline on the pattern and amount of sweetened ethanol and water intake in fluid restricted long-evans rats. following daily injections, 2-h two-bottle choice tests were conducted with water and an ethanol solution (15% ethanol v/v in 5% sucrose w/v). during a 20-day acquisition phase (experiment 1), ethanol intake patterns and amounts did not differ between saline (n = 6) and morphi ... | 1992 | 1418670 |
| tumor necrosis factor and chemotherapeutic agents. potentiation of cytotoxicity with interferon gamma. | this study examined combinations of the recombinant human cytokines, tumor necrosis factor alpha and interferon gamma, with doxorubicin and dactinomycin as well as other drugs on six squamous cell carcinoma cell lines of head and neck origin using the 3(4,5-dimethylthiazol-2-yl)2,5-diphenyl-tetrazolium bromide proliferation assay. interferon gamma significantly enhanced the cytotoxicity of tumor necrosis factor alpha with dactinomycin on all six cell lines investigated, while in four of six cell ... | 1992 | 1418895 |
| reconstitution and kinetics of vacuolar h(+)-translocating atpases from plants and animals. | | 1992 | 1426540 |
| effect of ethylenediamine di-o-hydroxyphenylacetic acid and transferrin on the growth of some bacterial strains in vitro. | the ability of some bacterial strains to obtain iron from ethylenediamine di-o-hydroxyphenylacetic acid (edda) or iron-free transferrin, and accordingly grow in their presence, was studied. growth of staphylococcus aureus and yersinia pseudotuberculosis was inhibited by edda or by iron-free transferrin. growth of streptococcus faecalis, however, was inhibited by iron-free transferrin, but not by edda. the other bacterial strains, i.e.; escherichia coli, salmonella typhimurium and shigella dysent ... | 1992 | 1435350 |
| loss of the pigmentation phenotype in yersinia pestis is due to the spontaneous deletion of 102 kb of chromosomal dna which is flanked by a repetitive element. | the pigmentation (pgm+) phenotype of yersinia pestis encompasses a variety of different physiological traits, all of which are missing in pgm- mutants. we have previously shown that loss of the pgm+ phenotype is accompanied by the spontaneous deletion of at least 45 kb of chromosomal dna, referred to as the pgm locus. using chromosomal walking, we have now mapped the full extent of the pgm locus in y. pestis strain kim6+. our results indicate that the locus spans 102 kb of dna which is absent in ... | 1992 | 1447977 |
| [a new r-plasmid from yersinia pseudotuberculosis]. | yersinia pseudotuberculosis strain 140-p isolated from soil in the far east was found to harbour an r-plasmid different from the plasmids that had been isolated from the bacteria previously. a new r-plasmid pld140 is conjugation proficient and codes for the cellular resistance to streptomycin, tetracycline and sulfonamides. the plasid belongs to incompatibility group incp. its restriction endonucleases bamhi and sali profile is different from the ones of the plasmids belonging to the rp4 family. | 1992 | 1454082 |
| structure of the proteolytic fragment f34 of calmodulin in the absence and presence of mastoparan as revealed by solution x-ray scattering. | the solution x-ray scattering technique has been applied to examine the conformations of the proteolytic fragment f34 (78asp-148lys) of calmodulin in the absence of both ca2+ and mastoparan, in the presence of ca2+ only, and in the presence of both ca2+ and mastoparan. the radius of gyration and the molecular weight for the f34 fragment increased by 1.1 +/- 0.3 a and 19%, respectively, upon binding of both 2 mol of ca2+/mol to the f34 fragment and mastoparan to form the tertiary complex. a small ... | 1992 | 1457423 |
| anti-enterobacteria antibodies in psoriatic arthritis. | the occurrence of certain antibacterial antibodies was studied in the sera of 22 healthy donors (hd) and 66 patients with different diseases. the cases investigated included 22 rheumatoid arthritis (ra), 22 non-arthritic-psoriasis (nap), and 22 psoriatic arthritis (pa) patients. a complement fixation test was used with yersinia enterocolitica 0:3 type (yec), yersinia pseudotuberculosis (ypt), campylobacter jejuni (cj), and campylobacter fetus (cf) antigens; the detection of anti-chlamydia tracho ... | 1992 | 1458698 |
| analysis of two human monoclonal antibodies against melanoma. | b cells derived from peripheral-blood lymphocytes (pbl) and tumor-infiltrating lymphocytes (til) from a patient with a high serum antibody titer to autologous melanoma were transformed with epstein-barr virus (ebv) and evaluated for reactivity against autologous tumor. b cells producing antibody reactive with autologous tumor and unreactive with normal fibroblasts were detected both in til and in pbl. one cell line derived from pbl and another derived from til sustained production of tumor-react ... | 1992 | 1459738 |
| [associations of the soil amoeba hartmannella rhysodes with the bacterial causative agents of plague and pseudotuberculosis in an experiment]. | some aspects of relationships between soil ameba and the causative agents of plague and pseudotuberculosis, capable of forming natural associations, are considered. ameba can phagocytose bacteria causing plague and pseudotuberculosis. cases of the preservation of individual bacterial cells at the stationary phase and in precysts of amebae have been registered. | 1992 | 1481595 |
| drug delivery optimization through bayesian networks. | this paper describes how bayesian networks can be used in combination with compartmental models to plan recombinant human erythropoietin (r-huepo) delivery in the treatment of anemia of chronic uremic patients. past measurements of hematocrit or hemoglobin concentration in a patient during the therapy can be exploited to adjust the parameters of a compartmental model of the erythropoiesis. this adaptive process allows more accurate patient-specific predictions, and hence a more rational dosage p ... | 1992 | 1482938 |
| professional doc (pdoc): a software environment for authoring and consulting of electronic documentation. interactive diagnosis and therapy: a medical application. | professional doc (pdoc) is a software environment for electronic documentation authoring and consulting based on the hypermedia technology. it allows to manage different kinds of information (pictures, sounds, texts, graphics, video) organized according to hyperstructures. the user-friendliness and effectiveness of its man-machine interface, and its availability on the most common hardware platforms makes it a suitable product for a very broad category of users. interactive diagnosis and therapy ... | 1992 | 1483002 |
| in vitro characteristics of yersinia pseudotuberculosis of nonhuman primate origin. | six strains of serotypes 1 or 2 of y. pseudotuberculosis were isolated from dead squirrel monkeys, a cotton-top tamarin and a marmoset hybrid. all strains harboured a 71.6 kb plasmid, all were totally oxacillin-resistant and partially resistant to cephalosporins. biochemically, serotypes 1 and 2 differed from each other in their beta-galactosidase production in a nonfermenter system, whereas the lack of rhamnose, maltose, salicin and trehalose fermentation seemed to be attributable to technical ... | 1992 | 1486229 |
| yersinia enterocolitica serovar 1,2a,3 biovar 3 in chinchillas. | yersinia enterocolitica (serovar 1,2a, 3; biovar 3, autoagglutination test positive) was isolated in a chinchilla, in which autopsy showed the typical lesions associated with pseudotuberculosis. we assume that this "chinchilla type" of yersinia enterocolitica still persists among stock, more than thirty years after its discovery. a brief overview of the distribution of the pathogen in europe and north america is given. | 1992 | 1486240 |
| identification and validation of a new nursing diagnosis: sick role conflict. | the purpose of this research is to assess the presence, etiology, and effects of sick role conflict experienced by hospitalized patients. data from 99 hospitalized adult subjects were analyzed using a path analysis design. patients were interviewed at the bedside using a structured interview tool developed by the researchers. it was found that subjects who occupied multiple roles, who were not adequately prepared for hospitalization, and who had an acute rather than chronic illness were prone to ... | 1992 | 1489584 |
| in vitro kinetics of 8-methoxypsoralen penetration into human lymphoid cells. | extracorporeal photochemotherapy (ecpc) requires ex vivo uva irradiation of blood lymphocytes during the time of the theoretical peak 8-methoxsalen (8-mop) concentration. the aims of this study were to determine the mechanism of cellular uptake of 8-mop, its possible saturation and the time needed to reach maximal concentration (tmax) in lymphoid cells. 8-mop was measured by liquid chromatography in the supernatant of lymphoid cell suspensions incubated with a known amount of 8-mop. the kinetics ... | 1992 | 1489716 |
| comparison of the antipsoriatic efficacy of heliotherapy and ultraviolet b: a cross-over study. | ten psoriasis patients who underwent a 4-week heliotherapy period in the canary islands were treated with ultraviolet b (uvb) phototherapy at relapse, 2-24 months later. in both settings, psoriasis improvement was monitored using a psoriasis severity index (psi), and uv exposure was recorded with polysulphone films. in the 8 patients that could be evaluated, a median healing rate of 94% was recorded in the heliotherapy period, with a median cumulative uv dose of 46 erythemal units (eu). in the u ... | 1992 | 1489722 |
| characterization, specificity, and igv gene usage of anti-lymphocyte monoclonal antibodies from perinatal mice. | previous studies have shown that cd5+ b cells predominate during development of the immune system and frequently secrete self-reactive antibodies, some of which appear to influence the development of the adult b cell repertoire. in addition, we now show that a high frequency of perinatally derived antibodies react with lymphocytes. hybridomas derived from perinatal liver and splenic b cells and from spleens of adult balb/c and c57bl/6 mice were screened by immunofluorescence on thymocytes. anti- ... | 1992 | 1489727 |
| dna adduct measurements and tumor incidence during chronic carcinogen exposure in animal models: implications for dna adduct-based human cancer risk assessment. | | 1992 | 1489923 |
| degenerate pcr primers for the amplification of fragments from genes encoding response regulators from a range of pathogenic bacteria. | many bacterial responses to environmental stimuli are mediated by response regulators which coordinately regulate genes involved in particular adaptive responses. degenerate oligonucleotide primers were used to amplify by the polymerase chain reaction (pcr), fragments from genes encoding eleven novel response regulators. sequence and phylogenetic analysis revealed that phob, phop and creb gene fragments had been amplified from yersinia enterocolitica and yersinia pseudotuberculosis, and that a c ... | 1992 | 1490612 |
| characterization of an amorphous and soluble hemagglutinin from yersinia pseudotuberculosis. | yersinia pseudotuberculosis which were screened out depending on auto-agglutination and ca2+ dependency, were examined for their production of hemagglutinin (ha), and its purification and characterization were performed. the ha with a broad reactivity with various mammalian erythrocytes was recovered from the culture supernatant of these strains grown at 37 c but not 25 c. has from two strains, r148r and t1040, were purified by salt precipitation, gel filtration and anion-exchange chromatography ... | 1992 | 1491617 |
| cytometric and cytochemical study of peritoneal and alveolar macrophages from yersinia pseudotuberculosis infected ground squirrels (citellus citellus). | the enzymic activity (succinate dehydrogenase, acid and alkaline phosphatase) of alveolar and peritoneal macrophages as well as leucocytic reaction of ground squirrels infected with yersinia pseudotuberculosis (i and iii serovar) have been investigated in dynamics from the 1st up to the 30th day. the animals infected with iii serovar survived only to the 7th day, while those infected with i serovar survived up to the 30th day after inoculation. a massive influx of leucocytes having peak values ( ... | 1992 | 1492517 |
| [the possibility of yersinia pseudotuberculosis transmission via the soil-plant-animal chain (a preliminary report)]. | | 1992 | 1496869 |
| [experience with the use of an immunoglobulin erythrocyte preparation for the early laboratory diagnosis of pseudotuberculosis]. | an immunoglobulin erythrocytic preparation for the rapid detection of yersinia pseudotuberculosis was developed. the preparation was shown to be specific and sufficiently sensitive (8 x 10(5) microbial cells/ml). the preparation was used for the examination of 102 patients; in 62 of these patients the diagnosis was confirmed by means of common laboratory and clinical methods. y. pseudotuberculosis antigen was detected in the blood of 39 patients (63%), and in 12 patients (29.4%) the results of t ... | 1992 | 1496881 |
| the integrin-binding domain of invasin is sufficient to allow bacterial entry into mammalian cells. | yersinia pseudotuberculosis is able to enter normally nonphagocytic host cells by multiple pathways, the most efficient of which is mediated by invasin, a 986-amino-acid bacterial outer membrane protein. it has previously been shown that the c-terminal 192 amino acids of invasin are sufficient to bind mammalian cells. to determine if additional regions of the invasin protein are necessary to promote entry, we developed a novel assay that tests the ability of various invasin derivatives to confer ... | 1992 | 1500198 |
| direct isolation of yersinia pseudotuberculosis from fresh water in japan. | yersinia pseudotuberculosis was recovered from koh-treated precipitates of 20.6% of 500 freshwater samples. koh treatment of precipitates is a simple and expedient means of recovering y. pseudotuberculosis from such samples. | 1992 | 1514818 |
| growth of yersinia species in artificially contaminated blood bags. | transfusion blood bags were inoculated with less than 0.1 colony-forming units (cfu) of yersinia enterocolitica (serotypes o:3, o:5, and o:9) and yersinia pseudotuberculosis i per ml and stored at 4 degrees c for 41 days. during storage, samples were collected periodically and colony counts of serial dilutions were performed by plating on tryptic soy agar. after a lag-phase, the growth of yersinia species from less than 0.1 cfu per ml to 10(9) cfu per ml was observed in 3 of 10 bags. the data sh ... | 1992 | 1519331 |
| the influence of the stomach on crop function and feeding behavior in domestic turkeys. | we tested the hypotheses that motility of the crop and muscular stomach are coordinated and that the stomach exerts primary control over crop filling and emptying in domestic turkeys. simultaneous recordings of motility of the crop, esophagus, and stomach with implanted strain gauge transducers and visual observations of food passage using image intensification radiography revealed an inverse relationship between the frequency of stomach and crop contractions. artificially filling the stomach of ... | 1992 | 1523251 |
| identification of invasive yersinia species using oligonucleotide probes. | oligonucleotide probes directed to the inv and ail invasion genes of yersinia species were used to analyse yersiniae and non-yersiniae isolates by colony hybridization. the inv-3 probe, targeted to the inv gene of yersinia pseudotuberculosis, hybridized with all 48 hela cell-invasive y. pseudotuberculosis isolates examined; the pf-13 probe, specific for the ail gene of yersinia enterocolitica, identified all invasive strains (36 of 52) of y. enterocolitica tested. neither probe hybridized with n ... | 1992 | 1528199 |
| yersinia pseudotuberculosis and ovine abortion. | | 1992 | 1529512 |
| mechanistic studies of the biosynthesis of 3,6-dideoxyhexoses in yersinia pseudotuberculosis: purification and characterization of cdp-4-keto-6-deoxy-d-glucose-3-dehydrase. | cdp-4-keto-6-deoxy-d-glucose-3-dehydrase (e1) is a pmp-dependent enzyme which plays an essential role in c-o bond cleavage leading to the formation of 3,6-dideoxyhexoses. although e1 catalysis has long been recognized as a unique biological deoxygenation reaction, the catalytic mechanism of this unusual enzyme has never been fully elucidated. the lack of methods that would allow this enzyme's activity to be monitored directly has been an impediment to e1 purification and has consequently hampere ... | 1992 | 1536853 |
| mechanistic and stereochemical studies of a unique dehydration catalyzed by cdp-4-keto-6-deoxy-d-glucose-3-dehydrase: a pyridoxamine 5'-phosphate dependent enzyme isolated from yersinia pseudotuberculosis. | cdp-4-keto-6-deoxy-d-glucose-3-dehydrase (e1) purified from yersinia pseudotuberculosis is a pyridoxamine 5'-phosphate (pmp) dependent enzyme which catalyzes the c-o bond cleavage at c-3 of a cdp-4-keto-6-deoxy-d-glucose substrate, a key step in the formation of 3,6-dideoxyhexoses. since enzyme e1 utilizes the pmp cofactor in a unique manner, it is essential to establish its role in e1 catalysis. when an incubation was conducted in [18o]h2o, incorporation of 18o into positions c-3 and c-4 of the ... | 1992 | 1536854 |