| antibacterial effect of protamine in combination with edta and refrigeration. | the antimicrobial effect of protamine (clupeine) on a range of gram-positive and gram-negative foodborne pathogens and spoilage bacteria, was evaluated using an agar dilution assay and a broth dilution assay with alamar blue as growth indicator. protamine was tested alone at concentrations from 0 to 10,000 microg/ml, and in combination with edta (0.9 mm). assays were performed at 5 degrees c, 10 degrees c, 18 degrees c and 30 degrees c to test the effect of temperature. minimum inhibitory concen ... | 2001 | 11428574 |
| structure of the o-specific polysaccharide of citrobacter braakii o7a,3b,1c. | the following structure of the o-specific polysaccharide of citrobacter braakii o7a,3b,1c was established using sugar and methylation analyses and nmr spectroscopy, including 2d cosy, tocsy, noesy, and 1h, 13c heteronuclear single-quantum coherence (hsqc) experiments: (struture: see text). the main d-mannan chain of the polysaccharide studied has the same structure as the o-specific polysaccharide of escherichia coli o9, klebsiella pneumoniae o3, and hafnia alvei pcm 1223. | 2001 | 11454340 |
| delayed-onset postoperative endophthalmitis caused by hafnia alvei. | to identify hafnia alvei as an etiological factor of delayed-onset endophthalmitis. | 2001 | 11456025 |
| source and identification of histamine-producing bacteria from fresh and temperature-abused albacore. | histamine-producing bacteria were isolated from fresh and temperature-abused albacore using two different isolation procedures. typically, the bacterial isolates on niven's or modified niven's medium produced negligible or low levels of histamine (<300 ppm) in histamine enumeration broth. the most frequently found species using this approach was hafnia alvei. by prescreening on selective media (eosin methylene blue [emb] agar for enteric bacteria; deman rogosa sharpe agar for lactic acid bacteri ... | 2001 | 11456189 |
| bacteriological quality of aquacultured freshwater fish portions in prepackaged trays stored at 3 degrees c. | fresh trout fillets and salmon slices packed in trays were obtained from two multinational chain supermarkets and evaluated for freshness and bacteriological quality immediately after packaging and during storage at 3 degrees c. initial aerobic counts at 30 and 25 degrees c were significantly (p < 0.05) lower in trout fillets (5.27 +/- 0.57 and 4.87 +/- 0.80 log cfu/g, respectively) than in salmon slices, where levels in excess of 6 log cfu/g were found. in both products, initial enterobacteriac ... | 2001 | 11563518 |
| host and geographical factors influence the thermal niche of enteric bacteria isolated from native australian mammals. | the thermal profiles of 118 bacterial strains, representing six species of the family enterobacteriaceae, isolated from a variety of native australian mammals were determined under in vitro conditions. each of the bacterial species had a unique thermal profile and differed in their minimum or maximum temperature for growth and in their response to changing temperatures. the taxonomic classification of the host from which the bacterial strains were isolated explained a significant amount of the v ... | 2001 | 11742550 |
| the eaea gene is not found in hafnia alvei from patients with diarrhea in aragón, spain. | a total of 102 hafnia alvei clinical strains isolated from different patients with diarrhea has been tested, using polymerase chain reaction and dot-blot hybridization, for the enteropathogenic escherichia coli attaching and effacing a (eaea) gene to establish their role as a causative agent of diarrhea in our environment. none of them was positive for the eaea gene. we cannot consider the eaea gene as the virulence-associated factor implicated in the h. alvei strains isolated from diarrheal fec ... | 2001 | 11770828 |
| a case of acute cholecystitis due to aeromonas sobria and hafnia alvei from northern europe. | | 1998 | 11864254 |
| modification of niven's medium for the enumeration of histamine-forming bacteria and discussion of the parameters associated with its use. | the objective of this study was to improve niven's medium (nm) for the optimized enumeration of histamine-forming bacteria (hfb). the parameters modified related to solidification of the agar at low ph values (ph 5.3 to 5.8), incubation time (24, 48, and 72 h) and temperature (30 and 37 degrees c), number of colonies developed on the plate to allow enumeration of hfb, and color differentiation. strains of hfb, morganella morganii, klebsiella pneumoniae, and hafnia alvei were examined for their a ... | 2002 | 11899055 |
| comparison of fecal coliform agar and violet red bile lactose agar for fecal coliform enumeration in foods. | a 24-h direct plating method for fecal coliform enumeration with a resuscitation step (preincubation for 2 h at 37 +/- 1 degrees c and transfer to 44 +/- 1 degrees c for 22 h) using fecal coliform agar (fca) was compared with the 24-h standardized violet red bile lactose agar (vrbl) method. fca and vrbl have equivalent specificities and sensitivities, except for lactose-positive non-fecal coliforms such as hafnia alvei, which could form typical colonies on fca and vrbl. recovery of cold-stressed ... | 2002 | 11916678 |
| evolution of the gyrb gene and the molecular phylogeny of enterobacteriaceae: a model molecule for molecular systematic studies. | phylogenetic trees showing the evolutionary relatedness of enterobacteriaceae based upon gyrb and 16s rrna genes were compared. congruence among trees of these molecules indicates that the genomes of these species are not completely mosaic and that molecular systematic studies can be carried out. phylogenetic trees based on gyrb sequences appeared to be more reliable at determining relationships among serratia species than trees based on 16s rrna gene sequences. gyrb sequences from serratia spec ... | 2002 | 11931166 |
| [enterobacteria isolated from cockroaches (periplaneta americana) captured in a brazilian hospital]. | to isolate and identify microorganisms from cockroaches that were captured in a public hospital and to test the antimicrobial susceptibility of these microorganisms. | 2002 | 11939120 |
| the structure of the o-chain of the lipopolysaccharide of a prototypal diarrheagenic strain of hafnia alvei that has characteristics of a new species under the genus escherichia. | the structure of the o-polysaccharide of the lipopolysaccharide from a diarrheal strain isolated in bangladesh was studied with sugar, and methylation analysis, nmr spectroscopy, mass spectrometry and partial acid hydrolysis. the strain was first designated as hafnia alvei, but later found to be a possible new species in the genus escherichia. two different polysaccharides were detected, a major and a minor one. the structure of the major polysaccharide is given below, while the structure of the ... | 2002 | 12084070 |
| an analysis of human pathogens found in horse/mule manure along the john muir trail in kings canyon and sequoia and yosemite national parks. | to determine the prevalence of microorganisms that are potentially pathogenic for humans in horse/mule manure along the john muir trail (jmt). | 2002 | 12092962 |
| phenotypic and genotypic properties of the genus hafnia. | the present study characterised 73 hafnia alvei isolates and five escherichia isolates (originally identified as h. alvei) isolated from cases of diarrhoeal disease by the international centre for diarrhoeal disease research branch (icddrb) in bangladesh. based upon the hydrolysis of arbutin and aesculin and the fermentation of salicin and d-arabinose, four distinct biotypes could be recognised among the 73 h. alvei isolates tested; biotype 1 (d-(-)-arabinose-positive only) accounted for 75% of ... | 2002 | 12132774 |
| evolutionary relationship of alw26i, eco31i and esp3i, restriction endonucleases that recognise overlapping sequences. | type ii restriction endonucleases (enases) have served as models for understanding the enzyme-based site-specific cleavage of dna. using the knowledge gained from the available crystal structures, a number of attempts have been made to alter the specificity of enases by mutagenesis. the negative results of these experiments argue that the three-dimensional structure of dna-enase complexes does not provide enough information to enable us to understand the interactions between dna and enases in de ... | 2002 | 12172806 |
| gas formation in ground beef chubs due to hafnia alvei is reduced by multiple applications of antimicrobial interventions to artificially inoculated beef trim stock. | gas-forming microorganisms were isolated from gas-swollen ground beef chubs obtained from a commercial source and were phenotypically identified as hafnia alvei. in in situ experiments, the isolated h. alvei strains produced gas in inoculated irradiation-sterilized ground beef chubs. a five-strain cocktail of h. alvei isolates was inoculated on beef trim. the inoculated beef trim samples were treated with either a water wash (w) at 65 psi for five passes (a pass refers to the application of succ ... | 2002 | 12380755 |
| [bilateral lung tuberculosis and over-infection by hafnia alvei]. | | 2002 | 12481504 |
| conjugative plasmid mediated inducible nickel resistance in hafnia alvei 5-5. | hafnia alvei 5-5, isolated from a soil-litter mixture underneath the canopy of the nickel-hyperaccumulating tree sebertia acuminata (sapotaceae) in new caledonia, was found to be resistant to 30 mm ni(2+) or 2 mm co(2+). the 70-kb plasmid, pejh 501, was transferred by conjugation to escherichia coli, serratia marcescens, and klebsiella oxytoca. transconjugant strains expressed inducible nickel resistance to between 5 and 17 mm ni(2+), and cobalt resistance to 2 mm co(2+). a 4.8-kb sal- ecori fra ... | 2003 | 12730713 |
| escherichia albertii sp. nov., a diarrhoeagenic species isolated from stool specimens of bangladeshi children. | the taxonomic position of a group of five d-sorbitol- and lactose-negative enterobacterial isolates recovered from diarrhoeal stools of children at the international centre for diarrhoeal disease research, bangladesh (icddr,b), was investigated by dna-dna hybridization, phenotypic characterization and 16s rdna sequencing. these strains were originally identified as 'hafnia alvei-like' with the api 20e system but, in fact, show more phenotypic and genotypic resemblance to members of the genus esc ... | 2003 | 12807204 |
| [hafnia alvei bacteremia with liver and muscle abscess: a case report]. | hafnia alvei is a commensal organism of the digestive tract. it is best known as an agent of pediatric gastro-enteritis. in adults, this organism may exceptionally be responsible for extra-intestinal infections. in such cases, infections occur mainly in unusually susceptible patients, due to an underlying condition. | 2003 | 12876518 |
| [clinical and microbiological features of acute enteric mixed infection caused by hafnia alvei and rotavirus]. | the data obtained in the clinical and laboratory study of 72 hospitalized patients with acute enteric infection are presented. the observed outbreak was caused by h. alvei producing heat-stable enterotoxin. the role of this etiological agent is also confirmed by simultaneous occurrence of the disease after using the same foodstuff, a short incubation period, the severity of the course of the disease with pronounced symptoms of neurotoxicosis, a high detection rate of h. alvei in material taken f ... | 2003 | 12886610 |
| evaluation of increased incubation temperature and cefixime-tellurite treatment for the isolation of escherichia coli o157:h7 from minced beef. | to improve enrichment and isolation of escherichia coli o157:h7, this study evaluated increased incubation temperature and cefixime-tellurite (ct) on five strains of each of the following bacteria, e. coli, hafnia alvei, enterobacter spp., citrobacter freundii and e. coli o157:h7, and two strains of e. coli o157:nh7. these were grown in pure culture in lst broth with varying cefixime-tellurite concentrations. a range of incubation temperatures from 37 to 46 degrees c was investigated for the inh ... | 2003 | 12927704 |
| detection of morganella morganii, a prolific histamine former, by the polymerase chain reaction assay with 16s rdna-targeted primers. | a polymerase chain reaction (pcr) assay for the rapid and sensitive detection of the most prolific histamine former, morganella morganii, was developed. 16s rdna targeted pcr primers were designed, and the primer specificity and sensitivity of the pcr assay were evaluated. the 16s rdna sequence (1,503 bp) for m. morganii showed 95% identity to those for enteric bacteria, i.e., enterobacter spp., klebsiella spp., citrobacter spp., hafnia alvei, proteus spp., and providencia spp. the unique primer ... | 2003 | 12929824 |
| hafnia alvei strains possessing the alpha antigen of stamp and stone. | | 1961 | 13696727 |
| structure of the o-polysaccharide from the lipopolysaccharide of hafnia alvei strain pcm 1546. | an acidic o-polysaccharide isolated by mild acid hydrolysis from the lipopolysaccharide of hafnia alvei pcm 1546 is composed of d-gal, d-glc, d-glca, d-galnac and o-acetyl groups in the ratios 1:1:1:2:1.6. on the basis of sugar and methylation analyses along with 1d and 2d 1h and 13c nmr spectroscopy, the following structure of the pentasaccharide repeating unit of the polysaccharide was established: [see equation in text]. | 2003 | 14505884 |
| [nosocomial pneumonia caused by hafnia alvei]. | | 2003 | 14624659 |
| a molecular phylogeny of enteric bacteria and implications for a bacterial species concept. | a molecular phylogeny for seven taxa of enteric bacteria (citrobacter freundii, enterobacter cloacae, escherichia coli, hafnia alvei, klebsiella oxytoca, klebsiella pneumoniae, and serratia plymuthica) was made from multiple isolates per taxa taken from a collection of environmental enteric bacteria. sequences from five housekeeping genes (gapa, groel, gyra, ompa, and pgi) and the 16s rrna gene were used to infer individual gene trees and were concatenated to infer a composite molecular phylogen ... | 2003 | 14640415 |
| genetic and ecological structure of hafnia alvei in australia. | multilocus enzyme electrophoresis of 161 hafnia alvei isolates from 158 hosts and 3 water column samples collected in australia revealed that this species consists of two genetically distinct groups. the two groups of h. alvei differed significantly in their genetic structure and host distribution. the taxonomic class of the host but not geographic locality explained a significant proportion of the observed genetic and biochemical variation among strains within each genetic group. | 2003 | 14666987 |
| antimicrobial susceptibility patterns, beta-lactamases, and biochemical identification of yokenella regensburgei strains. | yokenella regensburgei is an opportunistic human pathogen that phenotypically resembles hafnia alvei. the susceptibility of 10 y. regensburgei strains to 75 antimicrobial agents was examined, applying a microdilution procedure in cation-adjusted mueller-hinton broth (camhb) and isosensitest broth (isb). beta-lactamases were characterized phenotypically with beta-lactamase activity and induction assays. genotypically, pcr experiments applying degenerated primer pairs for the detection of ampc bet ... | 2004 | 14761716 |
| [nosocomial hafnia alvei sepsis in a neonatal intensive care unit]. | hafnia alvei is a member of the enterobacteriaceae family, which is rarely associated with infection in pediatric patients and exceptionally in the neonatal period. infections caused by this organism are usually opportunistic. h. alvei bacteremias are mostly associated with abdominal disease or immunosuppression. we report four cases of nosocomial sepsis in preterm infants with positive blood cultures to h. alvei that were treated in our institution within a short period of time. | 2004 | 14987520 |
| chimeric nature of two plasmids of hafnia alvei encoding the bacteriocins alveicins a and b. | the complete nucleotide sequences of two bacteriocin-encoding plasmids isolated from hafnia alvei (palva and palvb) were determined. both plasmids resemble cole1-type replicons and carry mobilization genes, as well as colicin-like bacteriocin operons. these bacteriocins appear to be chimeras consisting of translocation domains from tol-dependent colicins, unique binding domains, and killing and immunity domains similar to those of the pore-forming colicin ia. just as is found for colicin ia, the ... | 2004 | 14996789 |
| late-onset neonatal sepsis due to hafnia alvei. | hafnia alvei infections are uncommon, and usually occur in patients with underlying illnesses, mainly adults. the authors describe a rare case of late-onset community-acquired neonatal sepsis in an infant without any underlying disease. the infant was successfully treated with cefotaxime. | 2004 | 15000567 |
| structure of the o-polysaccharide from the lipopolysaccharide of hafnia alvei strain pcm 1529. | the following structure of the pentasaccharide repeating unit of an acidic o-polysaccharide of hafnia alvei pcm 1529 was established by sugar and methylation analyses along with 1d and 2d 1h and 13c nmr spectroscopy: [carbohydrate structure: see text]. | 2004 | 15013412 |
| analysis of ampc beta-lactamase expression and sequence in biochemically atypical ceftazidime-resistant enterobacteriaceae from paediatric patients. | to analyse the variation of ampc beta-lactamase gene sequence and expression in biochemically atypical enterobacteriaceae isolates, and to identify them definitively. | 2004 | 15014062 |
| photobacterium phosphoreum caused a histamine fish poisoning incident. | an incident of histamine fish poisoning (hfp) occurred due to the consumption of iwashi maruboshi (dried sardine) in osaka, japan in march 2002. a histamine-producing bacterial strain, ys4-7, was isolated from iwashi maruboshi that contained 1700 mg of histamine per kilogram. this strain was identified as photobacterium phosphoreum by biochemical examinations and partial sequencing of 16s rdna. p. phosphoreum ys4-7 showed greater capability as a histamine producer at 4 and 12 degrees c than morg ... | 2004 | 15033270 |
| volatile compounds produced in cheese by enterobacteriaceae strains of dairy origin. | the formation of volatile compounds in fresh cheese by 10 enterobacteriaceae strains of dairy origin (4 hafnia alvei, 2 serratia liquefaciens, 1 enterobacter cloacae, 1 enterobacter sakazakii, and 2 escherichia coli strains) was investigated. small cheeses were made from pasteurized cow's milk separately inoculated with 1-3 x 10(3) cfu/ml of each of the enterobacteriaceae strains, with glucono-8-lactone added to achieve a ph value of 5.2 in the curds. all strains reached counts close to 10(8) cf ... | 2004 | 15035375 |
| dna based classification of food associated enterobacteriaceae previously identified by biolog gn microplates. | enterobacteriaceae are frequently isolated from food products and it is essential to have methods for correct identification for both food hygiene and epidemiology reasons. phenotypic methods are not always sufficient and have to be supplemented by dna based methods. in the present study, 70 strains of enterobacteriaceae derived from milk, fish and meat that had previously been identified by biolog gn microplates were genomically classified together with 15 representative type strains of species ... | 2004 | 15046311 |
| sanitary and bacteriological aspects of sewage treatment. | a study into the removal of contamination load and indicator bacteria was carried out in 1992-1996 in the mechanical, biological and chemical waste-water treatment plant wtp in lezany, in the county of reszel, in the province of warmia and mazury in poland. the results of chemical analyses found a high efficiency of removal of carbon compounds, cod (90%) and bod (98%), in the process of purification of household sewage. in addition, a high effectiveness of total nitrogen, on average 71%, and uns ... | 2003 | 15058814 |
| nucleotide sequence and expression of the ncr nickel and cobalt resistance in hafnia alvei 5-5. | the structural genes for the nickel and cobalt resistance of the conjugative plasmid pejh 501 of hafnia alvei 5-5, contained on a sali-ecori fragment of 4.8 kb, were cloned and sequenced. the dna sequence included five genes in the following order: ncra, ncrb, ncrc, ncry, and ncrx. the predicted amino acid sequences of ncra were homologous to the amino acid sequences of nreb of achromobacter xylosoxidans 31a. expression of ncr with the t7 rna polymerase-promoter system allowed escherichia coli b ... | 2004 | 15179604 |
| characterization of bacterial diversity in pulque, a traditional mexican alcoholic fermented beverage, as determined by 16s rdna analysis. | the bacterial diversity in pulque, a traditional mexican alcoholic fermented beverage, was studied in 16s rdna clone libraries from three pulque samples. sequenced clones identified as lactobacillus acidophilus, lactobacillus strain asf360, l. kefir, l. acetotolerans, l. hilgardii, l. plantarum, leuconostoc pseudomesenteroides, microbacterium arborescens, flavobacterium johnsoniae, acetobacter pomorium, gluconobacter oxydans, and hafnia alvei, were detected for the first time in pulque. identity ... | 2004 | 15183874 |
| presence of acylated homoserine lactones (ahls) and ahl-producing bacteria in meat and potential role of ahl in spoilage of meat. | quorum-sensing (qs) signals (n-acyl homoserine lactones [ahls]) were extracted and detected from five commercially produced vacuum-packed meat samples. ninety-six ahl-producing bacteria were isolated, and 92 were identified as enterobacteriaceae. hafnia alvei was the most commonly identified ahl-producing bacterium. thin-layer chromatographic profiles of supernatants from six h. alvei isolates and of extracts from spoiling meat revealed that the major ahl species had an r(f) value and shape simi ... | 2004 | 15240313 |
| hafnia alvei infection in pullets in italy. | this paper describes an outbreak of disease caused by hafnia alvei in pullets. cloudy swelling and the fatty degeneration of liver associated with splenic lymphocytic depletion were the most preminent lesions. the organism was identified by biochemical tests and by a hafnia-specific bacteriophage test. laying hens and pullets were infected experimentally with the organism by the oral and intraperitoneal route, and the clinical and pathological effects were similar to those observed in naturally ... | 2004 | 15276988 |
| detection of low levels of listeria monocytogenes cells by using a fiber-optic immunosensor. | biosensor technology has a great potential to meet the need for sensitive and nearly real-time microbial detection from foods. an antibody-based fiber-optic biosensor to detect low levels of listeria monocytogenes cells following an enrichment step was developed. the principle of the sensor is a sandwich immunoassay where a rabbit polyclonal antibody was first immobilized on polystyrene fiber waveguides through a biotin-streptavidin reaction to capture listeria cells on the fiber. capture of cel ... | 2004 | 15466560 |
| evaluation of the microscan esbl plus confirmation panel for detection of extended-spectrum beta-lactamases in clinical isolates of oxyimino-cephalosporin-resistant gram-negative bacteria. | we aimed to assess the performance of the microscan esbl plus confirmation panel using a series of 87 oxyimino-cephalosporin-resistant gram-negative bacilli of various species. | 2004 | 15471997 |
| the o-acetylation patterns in the o-antigens of hafnia alvei strains pcm 1200 and 1203, serologically closely related to pcm 1205. | serological tests revealed immunochemical similarities between the lipopolysaccharides of hafnia alvei strains pcm 1200, 1203 and 1205. immunoblotting and elisa showed cross-reactions between the strains. nmr spectroscopy showed that the o-deacetylated o-specific polysaccharides isolated from lipopolysaccharides of h. alvei strains pcm 1200 and 1203 possessed the same composition and sequence as the o-deacetylated o-specific polysaccharide of h. alvei strain pcm 1205, that is a glycerol teichoic ... | 2004 | 15476713 |
| antimicrobial effect of water extract of sumac (rhus coriaria l.) on the growth of some food borne bacteria including pathogens. | the antimicrobial effect of water extracts of sumac (rhus coriaria l.) at concentrations of 0.1%, 0.5%, 1.0%, 2.5% and 5.0% (w/v), non-neutralized and after neutralization to ph 7.2+/-0.1, was studied on the growth of 12 bacterial strains (six gram positive strains and six gram negative strains), mostly food borne including pathogens. it was found to be effective against all the test organisms with gram positive strains being more sensitive than gram negative strains. significant differences (p< ... | 2004 | 15527919 |
| evolutionary genetics of a new pathogenic escherichia species: escherichia albertii and related shigella boydii strains. | a bacterium originally described as hafnia alvei induces diarrhea in rabbits and causes epithelial damage similar to the attachment and effacement associated with enteropathogenic escherichia coli. subsequent studies identified similar h. alvei-like strains that are positive for an intimin gene (eae) probe and, based on dna relatedness, are classified as a distinct escherichia species, escherichia albertii. we determined sequences for multiple housekeeping genes in five e. albertii strains and c ... | 2005 | 15629933 |
| [histopathologic studies in experimental uveitis]. | experimental uveitis is one of the main models in the diseases of autoimmunological background. the purpose of this paper was to analyze the quantitative histological changes in the experimental uveitis, induced by different types of homogenous endotoxin salts of havnia alvei. we studied 74 eyes of lewis rats (males) divided into 4 groups. each group received a homogenous salt of havnia alvei in a single subcutaneous injection. in the 1 group-lps ca++, in 2 group-lps na++, in 3 group-lps 981, in ... | 2004 | 15636226 |
| structure of the o-polysaccharide of hafnia alvei strain pcm 1189 that has hexa- to octasaccharide repeating units owing to incomplete glucosylation. | the o-polysaccharide of hafnia alvei pcm 1189 consists of d-glucose, d-galactose, d-galnac and d-glca and lacks the strict regularity. the intact and carboxyl-reduced polysaccharides as well as oligosaccharides obtained by partial acid hydrolysis were studied by chemical and enzymatic analyses, methylation and nmr spectroscopy. the following structure was established for the o-polysaccharide, which is built up of branched hexa- to octasaccharide repeating units differing in the number of lateral ... | 2005 | 15639246 |
| spontaneous bacterial peritonitis due to hafnia alvei in a patient with peritoneal mesothelioma. | | 2005 | 15756379 |
| natural antimicrobial susceptibility patterns and biochemical identification of escherichia albertii and hafnia alvei strains. | bangladeshi diarrheagenic hafnia alvei-like strains have been described recently as the new species escherichia albertii (int j syst evolut microbiol. 2003;53:807-810). the natural susceptibility of 21 e. albertii and 76 h. alvei strains to 69 antimicrobial agents was examined, applying a microdilution procedure in isosensitest broth (for all the strains) and cation-adjusted mueller-hinton broth (for some strains). examining the phenotypic features of both taxa with commercial identification sys ... | 2005 | 15766600 |
| structures of the biological repeating units in the o-chain polysaccharides of hafnia alvei strains having a typical lipopolysaccharide outer core region. | earlier, the structures of the o-chain polysaccharides of the lipopolysaccharides (lps) of a number of hafnia alvei strains have been established. however, it remained unknown, which is the first and the last monosaccharide of the o-chain. this is defined by the structure of the so-called biological repeating unit (o-unit), which is pre-assembled and then polymerised in the course of biosynthesis of bacterial polysaccharides by the wzy-dependent pathway. now we report on the structures of the o- ... | 2005 | 15961298 |
| the effect of electron beam irradiation on the survival of salmonella enterica serovar typhimurium and psychrotrophic bacteria on raw chicken breasts stored at four degrees celsius for fourteen days. | the effect of high-energy electron beam irradiation on the survival of salmonella enterica serovar typhimurium and psychrotrophic bacteria on commercial chicken breast meat was evaluated. fresh chicken breast meat was purchased from a local poultry processor, inoculated with 8 log10 cfu/ml salmonella, packaged in styrofoam trays and over wrapped with a polyvinyl chloride film, and subjected to 0, 1, 2, or 3 kgy of irradiation. the packaged samples were stored at 4 degrees c and analyzed for salm ... | 2005 | 15971537 |
| identification of two distinct hybridization groups in the genus hafnia by 16s rrna gene sequencing and phenotypic methods. | a collection of 52 strains belonging to the hafnia alvei complex were subjected to molecular (16s rrna gene sequencing) and biochemical analysis. based upon 16s rrna gene sequencing results, two genetic groups were identified which correspond with previously recognized dna hybridization group 1 (atcc 13337(t) and atcc 29926; n = 23) and dna hybridization group 2 (atcc 29927; n = 29). of 46 biochemical tests used to characterize hafniae, 19 reactions (41%) yielded variable results. of these 19 te ... | 2005 | 16000455 |
| [hafnia alvei in a rare case of severe pneumonia in a postanesthesia recovery unit]. | an 85-year-old man with a history of moderate chronic obstructive pulmonary disease in treatment with anticholinergic drugs was admitted to the recovery unit for postoperative monitoring after right inguinal herniorrhaphy surgery and removal of a strangulated intestinal section. on the third day after surgery the patient developed radiographic signs consistent with pneumonia and required mechanical ventilation. two blood cultures and a respiratory secretion sample grew a microorganism identified ... | 2005 | 16038176 |
| virulence factors and pathogenicity of hafnia alvei for gilthead seabream, sparus aurata l. | virulence factors (eae gene, haemolytic capacity, fimbriae, resistance to the bactericidal effect of serum, siderophore production) and pathogenicity for gilthead seabream, sparus aurata l., were analysed for 23 hafnia alvei strains. none of the strains used in ld50 studies were lethal for seabream at doses as high as >10(8) cfu ml(-1). in chronic challenge studies differences in severity of the inflammatory response were observed between strains. on the basis of correlation of the inflammatory ... | 2005 | 16083446 |
| isolation of vibrio cholerae from neonates admitted to an urban diarrhoeal diseases hospital in bangladesh. | very little is known about vibrio cholerae infection in neonates with diarrhoea. | 2005 | 16156982 |
| abdominal abscess and hafnia alvei septicemia occurring during the aplastic phase after autologous stem-cell transplantation in a patient with diffuse large b-cell lymphoma. | hafnia alvei is a motile gram-negative bacterium that is rarely isolated from human specimens, but that sometimes can be found as part of the gastrointestinal flora. here we report a rare case of hafnia alvei septicemia with an abdominal abscess in a 60-year-old woman with diffuse large b-cell lymphoma involving the spleen, liver, and then lymph nodes. she initially received a splenectomy, and, over a 2-year period, four courses of chemotherapy. after achieving complete remission status, she und ... | 2004 | 16163467 |
| clinical characteristics of nosocomial and community-acquired extraintestinal infections caused by hafnia alvei. | 36 episodes (25 nosocomial and 11 community-acquired) of infections caused by hafnia alvei showed that this bacterium is responsible for serious infections in adults, specially in hospitalized patients with underlying chronic diseases, subjected to invasive procedures or even under antibiotic treatment. | 2005 | 16308222 |
| biochemical and molecular characterization of obesumbacterium proteus, a common contaminant of brewing yeasts. | we have evaluated the effectiveness of api 20e, biolog testing, plasmid profiling, ribotyping, and enteric repetitive intergenic consensus (eric)-pcr to characterize, classify, and differentiate nine bacterial isolates of the common brewery contaminant obesumbacterium proteus. of the five typing techniques, biolog testing, plasmid profiling, and eric-pcr provided the most differentiation, and api 20e testing and ribotyping were relatively indiscriminate. the molecular biology approach of eric-pc ... | 1994 | 16349260 |
| the genus hafnia: from soup to nuts. | the genus hafnia, a member of the family enterobacteriaceae, consists of gram-negative bacteria that are occasionally implicated in both intestinal and extraintestinal infections in humans. despite the fact that the genus currently contains only a single species (h. alvei), more extensive phylogenetic depth (two or more species) is apparent based upon dna relatedness and 16s rrna gene sequencing studies. hafnia causes a variety of systemic infections, including septicemia and pneumonia; however, ... | 2006 | 16418520 |
| [beta-lactamases of gram negative bacteria: never-ending clockwork!]. | the acquired resistance against the wide-spectrum and highly stable beta-lactams including third-generation cephalosporins (3gc) and carbapenems is constinuously increasing and widespead with the discovery of various plasmid-encoded, or genes cassette or integrons coding for a novel beta-lactamase, always a major mechanism of resistance. to explain resistance against 3gc, with the continuing story with tem and shv mutated enzymes, several types of esbl (class a) emerge the ctx-m type, at least c ... | 2006 | 16420989 |
| characterization of the brewery spoilage bacterium obesumbacterium proteus by automated ribotyping and development of pcr methods for its biotype 1. | to study the ability of automated ribotyping to characterize obesumbacterium proteus and hafnia alvei, to design primers and to evaluate standard end-point and real-time pcr for the detection of o. proteus biotype 1 in beer and in brewers's yeast-containing samples. | 2006 | 16430517 |
| nickel-resistant bacteria from anthropogenically nickel-polluted and naturally nickel-percolated ecosystems. | dna fragments harboring the nickel resistance determinants from bacteria isolated from anthropogenically polluted ecosystems in europe and zaire were compared with those harboring the nickel resistance determinants from bacteria isolated from naturally nickel-percolated soils from new caledonia by dna-dna hybridization. the biotinylated dna probes were derived from the previously described alcaligenes eutrophus ch34, alcaligenes xylosoxidans 31a, alcaligenes denitrificans 4a-2, and klebsiella ox ... | 1995 | 16535048 |
| [bacteremia caused by hafnia alvei in an intensive care neonatal unit]. | | 2006 | 16650371 |
| population-based laboratory surveillance of hafnia alvei isolates in a large canadian health region. | hospital-based series have characterized hafnia alvei primarily as an infrequent agent of polymicrobial nosocomial infections in males with underlying illness. | 2006 | 16707023 |
| [obstructive pyocholecystitis and septicaemia related from hafnia alvei: influence of diabetes mellitus]. | | 2006 | 16716560 |
| a complex of lactoferrin with monophosphoryl lipid a is an efficient adjuvant of the humoral and cellular immune response in mice. | our recent investigations demonstrated adjuvant properties of lactoferrin (lf). other studies proved efficacy and safety of monophosphoryl lipid a (mpl) as an adjuvant in humans. in an attempt to construct more efficient and safer adjuvants, we evaluated the activity of lf-mpl complex, formed by incubation of lf and mpl from hafnia alvei at 20:1 w/w ratio, and verified its characteristics by sds-page analysis. binding kinetics was determined by surface plasmon resonance analysis using a biacore ... | 2006 | 16838169 |
| isolation of toxigenic hafnia alvei from a probable case of hemolytic uremic syndrome. | an 11-year-old girl presented to a central california children's hospital with a 3-day history of erythematous lesions on her forehead, neck, and trunk, abdominal pain, persistent emesis, and decreased urinary output. one day prior to admission she had a mild bout of diarrhea with a small amount of blood in her stool. upon admission her condition rapidly worsened with acute renal failure, anemia, and thrombocytopenia. one of the possible causes of this condition included hemolytic uremic syndrom ... | 2006 | 16896583 |
| microbiological characteristics of "androlla", a spanish traditional pork sausage. | counts of total aerobic mesophilic microflora, lactic acid bacteria, salt-tolerant microflora, enterobacteriaceae, enterococci, moulds and yeasts, and staphylococci, and some physico-chemical parameters (total solids, nacl and nitrate contents and ph and aw values) were determined in 20 units of "androlla", a traditional dry-fermented sausage made in the nw of spain. in general, high counts of all the investigated microbial groups were observed, with average values of 8.99 +/- 0.46 log cfu/g for ... | 2007 | 16943094 |
| genetic environment of acquired bla(acc-1) beta-lactamase gene in enterobacteriaceae isolates. | we studied the genetic organization of bla(acc-1) in 14 isolates of enterobacteriaceae from france, tunisia, and germany. in a common ancestor, isecp1 was likely involved in the mobilization of this gene from the hafnia alvei chromosome to a plasmid. other genetic events involving insertion sequences (particularly is26), transposons (particularly tn1696), or suli-type integrons have occurred, leading to complex genetic environments. | 2006 | 16982793 |
| increased serum iga and igm against lps of enterobacteria in chronic fatigue syndrome (cfs): indication for the involvement of gram-negative enterobacteria in the etiology of cfs and for the presence of an increased gut-intestinal permeability. | there is now evidence that chronic fatigue syndrome (cfs) is accompanied by immune disorders and by increased oxidative stress. the present study has been designed in order to examine the serum concentrations of iga and igm to lps of gram-negative enterobacteria, i.e. hafnia alvei; pseudomonas aeruginosa, morganella morganii, proteus mirabilis, pseudomonas putida, citrobacter koseri, and klebsiella pneumoniae in cfs patients, patients with partial cfs and normal controls. we found that the preva ... | 2007 | 17007934 |
| etiologic agents of cervicovaginitis in turkish women. | to investigate the distribution of microbiologic agents causing cervicovaginitis. | 2006 | 17013472 |
| misidentification of a mucoid strain of salmonella enterica serotype choleraesuis as hafnia alvei by the vitek gni+ card system. | nontyphoidal salmonellae are among the most common causes of bacterial gastroenteritis worldwide. they are also notable causes of extraintestinal infections, including bacteremia and vascular infections. salmonella enterica serotype choleraesuis is typically associated with invasive infections. we report a patient who had an infected intra-abdominal aortic aneurysm due to an unusually mucoid strain of salmonella enterica serotype choleraesuis. the isolate was erroneously identified as hafnia alv ... | 2006 | 17050821 |
| structure and serological analysis of the hafnia alvei 481-l o-specific polysaccharide containing phosphate in the backbone chain. | the lipopolysaccharide was extracted from cells of hafnia alvei 481-l bacterial strain and, after mild acid hydrolysis, the o-specific polysaccharide was isolated and characterised. on the basis of chemical analyses and nmr spectroscopic studies of the polysaccharide and oligosaccharides obtained after smith degradation, or hydrogen fluoride treatment, it was found that the repeating unit of the o-specific polysaccharide is a phosphorylated hexasaccharide: [see text]. the biological repeating un ... | 2006 | 17081508 |
| occurrence of proteolytic activity and n-acyl-homoserine lactone signals in the spoilage of aerobically chill-stored proteinaceous raw foods. | proteolytic pseudomonads dominate the spoilage flora of aerobically chill-stored proteinaceous raw foods. proteolysis during spoilage of these food systems affects both food quality and the dynamics of the bacterial community because it increases the availability of nutrients to the community as a whole. quorum sensing, or cell-cell signaling, is associated closely with ecological interactions among bacteria in mixed communities. the potential role of quorum sensing in proteolytic food spoilage ... | 2006 | 17133818 |
| examination of the new alpha-(2'z-fluoro)vinyl trigger with lysine decarboxylase: the absolute stereochemistry dictates the reaction course. | | 2007 | 17212389 |
| hafnia alvei endocarditis following pyelonephritis in a permanent pacemaker carrier. | | 2007 | 17256223 |
| [pyrazinamidase activity of bacteria from enterobacteriaceae family as characteristic for taxonomy]. | pyrazinamidase activity in 330 strains of bacteria from enterobacteriaceae family (14 genus, 27 species) has been assessed. pyrazinamidase activity detected in species from following genuses: citrobacter, escherichia, klebsiella, kluyvera, morganella, providencia, raourtella, salmonella, shigella, and also in proteus mirabilis, and nonpathogenic serovars of yersinia enterocolitica, y. frederiksenii. pirasinamidase was absent in serratia (s. marcescens, s. liguefaciens), hafnia alvei, p. vulgaris ... | 2006 | 17297889 |
| young-infant sepsis combined with urinary tract infection due to hafnia alvei. | hafnia alvei infections are uncommon and occur mainly in adult patients featuring underlying illnesses. its isolation in pediatric cases is even more unusual. we report a rare case of sepsis combined with urinary tract infection caused by h. alvei in a 39-day-old infant who did not appear to feature any underlying disease. the infant was successfully treated with ceftriaxone over a 14-day period. in this case, we want to remind clinicians that the possibility of an extraintestinal invasive infec ... | 2007 | 17493908 |
| [genetic markers of pathogenicity of conditionally pathogenic enterobacteria isolated from children with diarrheal diseases]. | in the process of examination of 89 children from different age groups with diarrheal disease caused by bacteria from enterobacteriaceae family 89 microorganisms were isolated including klebsiella spp. (37 isolates), citrobacter spp. (9 isolates), enterobacter spp. (17 isolates), hafnia alvei (1 isolate), morganella morganii (11 isolates), proteus spp. (14 isolates). presence of genes associated with pathogenicity islands (pais): hlya, hlyb (hemolysin), sfag (fimbria antigen type s), cnf1 (cytot ... | 2007 | 17523471 |
| absidia corymbifera in an immune competent accident victim with multiple abdominal injuries: case report. | we report a case of mucormycosis in a healthy 17-year-old accident victim with multiple abdominal injuries which was caused by infection with absidia corymbifera, a ubiquitous saphrophyte in the ground. | 2007 | 17531089 |
| failure to detect salmonella species in a population of wild tuatara (sphenodon punctatus). | to assess the prevalence of faecal excretion of salmonella serovars by wild tuatara (sphenodon punctatus) on stephens island, new zealand. | 2007 | 17534416 |
| evidence of temporal postdischarge decontamination of bacteria by gliding electric discharges: application to hafnia alvei. | this study aimed to characterize the bacterium-destroying properties of a gliding arc plasma device during electric discharges and also under temporal postdischarge conditions (i.e., when the discharge was switched off). this phenomenon was reported for the first time in the literature in the case of the plasma destruction of microorganisms. when cells of a model bacterium, hafnia alvei, were exposed to electric discharges, followed or not followed by temporal postdischarges, the survival curves ... | 2007 | 17557841 |
| first total synthesis of a pentasaccharide repeating unit of the o-antigen of hafnia alvei pcm 1529. | a pentasaccharide repeating unit of the o-antigen of hafnia alvei has been synthesized in a concise manner. high yielding glycosylation steps and minimum number of protecting group manipulation steps are the key features of this synthesis. a two-step, one-pot phase transfer oxidation protocol has been applied for the preparation of d-galacturonic acid. | 2008 | 17659441 |
| plasmid-encoded acc-4, an extended-spectrum cephalosporinase variant from escherichia coli. | acc-4, an omega loop mutant (val(211)-->gly) of the hafnia alvei-derived cephalosporinase acc-1, was encoded by an escherichia coli plasmid. the genetic environment of bla(acc-4) shared similarities with plasmidic regions carrying bla(acc-1). kinetics of beta-lactam hydrolysis and levels of resistance to beta-lactams showed that acc-4 was more effective than acc-1 against expanded-spectrum cephalosporins. | 2007 | 17664321 |
| contamination of milk by enterococci and coliforms from bovine faeces. | to determine the contribution of enterococci and coliforms from bovine faeces and teats to contamination of raw milk. methods: putative enterococci (n = 301) and coliforms (n = 365) were isolated from bovine faeces (n = 20), cows' teats (n = 20), the raw milk (n = 1) and the milking environment (n = 4) on one farm. the clonal relationships of each bacterial group were investigated using pulsed-field gel electrophoresis of genomic macrorestriction fragments. representatives of the different clust ... | 2007 | 17953550 |
| microbial interactions within a cheese microbial community. | the interactions that occur during the ripening of smear cheeses are not well understood. yeast-yeast interactions and yeast-bacterium interactions were investigated within a microbial community composed of three yeasts and six bacteria found in cheese. the growth dynamics of this community was precisely described during the ripening of a model cheese, and the lotka-volterra model was used to evaluate species interactions. subsequently, the effects on ecosystem functioning of yeast omissions in ... | 2008 | 17981942 |
| concise chemical synthesis of a tetrasaccharide repeating unit of the o-antigen of hafnia alvei 10457. | concise chemical synthesis of a tetrasaccharide repeating unit of the o-antigen of hafnia alvei 10457 is reported. construction of the tetrasaccharide as its 4-methoxyphenyl glycoside was achieved by condensation of less abundant monosaccharide units such as, d-galactofuranose, n-acetyl-d-galactosamine and n-acetylneuraminic acid. the synthetic strategy consists of the preparation of suitably protected required monosaccharide intermediates from the commercially available reducing sugars and high ... | 2008 | 18074224 |
| influence of environmental conditions on biofilm formation by hafnia alvei strains. | hafnia alvei is considered an opportunistic pathogen of animal and humans, affecting a wide range of homeothermic and poikilothermic hosts with different body temperatures. in this work, h. alvei strains isolated from different sources were studied with regard to their capacity to form biofilms under different environmental conditions. strain, growth phase, temperature and culture media dependent changes of biofilm formation were semiquantitatively monitored using a microtiter plate method. our ... | 2008 | 18093756 |
| method for reliable isolation of lactobacillus sakei strains originating from tunisian seafood and meat products. | in tunisia, several food products derived from meat or seafood are naturally processed, without any addition of bacterial starters. such fermented, dried-cured, salted, or marinated products, as well as the raw meat or fish may thus provide a source to isolate the natural microflora colonizing such environments. we isolated lactic acid bacteria from a representative range of flesh-foods sold or manufactured in different parts of tunisia, and selectively searched for lactobacillus sakei, a lactic ... | 2008 | 18155310 |
| antibiotic resistance and adherence properties of hafnia alvei clinical isolates: a 19-month study in the hospital of orléans, france. | we studied the epidemiology of antibiotic resistance and adherence properties among all hafnia alvei clinical isolates collected from august 2003 to february 2005 from patients hospitalized in the hospital of orléans, france. the isolates were typed by random amplified polymorphic dna (rapd), screened for antibiotic resistance and bacterial adherence to a549 respiratory and t24 bladder cells. six intestinal, 3 respiratory, and 8 isolates from different body sites were collected. a total of 12 ra ... | 2007 | 18230550 |
| lactoferrin-monophosphoryl lipid a complex enhances immunity of mice to plesiomonas shigelloides cnctc 138/92. | our previous study showed the efficacy of lactoferrin-monophosphoryl lipid a isolated from hafnia alvei lps complex (lf-mpl(h.a.)) as an adjuvant in stimulation of humoral and cellular immune response in mice to conventional antigens and a lower pyrogenicity of the complex as compared with mpl(h.a.) alone. in the present investigation we demonstrated that lf-mpl(h.a.) complex enhanced the immunity of balb/c mice immunized with plesiomonas shigelloides cnctc 138/92 bacterial vaccine, against p. s ... | 2008 | 18231655 |
| [gene cloning, expression and characterization of a novel phytase from hafnia alvei]. | a gene appa encoding a novel phytase was firstly cloned from hafnia alvei by pcr and sequenced. the gene was consisted of 1335 bp, encoding 444 amino acids. the calculated molecular weight of the mature appa was about 45.2 kd. the gene appa was expressed in e. coli bl21 (de3). recombinant appa was purified and its enzymatic properties were determined. the optimum ph for the enzyme was 4.5 and the optimum temperature was 60 degrees c. the ph stability of r-appa is good, the relative phytase activ ... | 2007 | 18257229 |
| the gut-brain barrier in major depression: intestinal mucosal dysfunction with an increased translocation of lps from gram negative enterobacteria (leaky gut) plays a role in the inflammatory pathophysiology of depression. | there is now evidence that major depression (mdd) is accompanied by an activation of the inflammatory response system (irs) and that pro-inflammatory cytokines and lipopolysacharide (lps) may induce depressive symptoms. the aim of the present study was to examine whether an increased gastrointestinal permeability with an increased translocation of lps from gram negative bacteria may play a role in the pathophysiology of mdd. toward this end, the present study examines the serum concentrations of ... | 2008 | 18283240 |
| identification of a non-pathogenic surrogate organism for chlorine dioxide (clo2) gas treatment. | the identification of non-pathogenic surrogate microorganisms is beneficial for determining and validating the efficacy of antimicrobial treatments in food manufacturing environments. a surrogate organism was identified to aid in the decontamination process of fresh produce when treated with chlorine dioxide (clo(2)) gas. thirty-two known strains of pathogenic and non-pathogenic microorganisms and seven unknown microbial isolates from mushroom, tomatoes, and strawberries were evaluated. the prim ... | 2008 | 18456115 |
| vitek 2 failure in screening hafnia alvei inducible beta-lactam resistance. | | 2008 | 18511150 |
| molecular characterization of a cryptic plasmid from the psychrotrophic antarctic bacterium pseudoalteromonas sp. 643a. | we report the identification and nucleotide sequence analysis of pkw1, a plasmid of the psychrotrophic bacterium pseudoalteromonas sp. 643a isolated from the stomach of antarctic krill euphasia superba. pkw1 consists of 4583 bp, has a g+c content of 43% and seven putative open reading frames (orfs). the deduced amino acid sequence from orf-1 shared significant similarity with the plasmid replicase protein of psychrobacter cryohalolentis, strain k5. the dna region immediately downstream of the or ... | 2008 | 18611409 |
| an unexpected isolate of hafnia alvei with reduced susceptibility to cefoxitin. | | 2008 | 18653239 |