| microbiological quality and public health significance of ice-cream. | fifty samples of ice-cream were subjected to microbiological examination. of these 72% had total viable count over 10(6)/g while 66% had coliform count between 10(2)-10(3)/g. the micro-organisms isolated were escherichia coli (46%), enterobacter aerogenes (34%), staphylococcus aureus (26%), proteus species (16%), streptococcus faecalis (12%), citrobacter species (10%) and bacillus cereus (4%). the results showed that commercially prepared ice-cream sold in the market was not satisfactory for hum ... | 1989 | 2501524 |
| urinary tract infection. | over two years, 9892 mid-stream urine samples from patients attending the aga khan university hospital, karachi were cultured. significant bacterial growth was seen in 23.5% samples. further identification of these organisms revealed 40% of e. coli, 16% pseudomonas aeruginosa, 11% klebsiella aerogenes, 5.0% enterobacter sp., 13% proteus sp., 4.0% serratia liquifaciens, 1.0% acinetobacter sp., 3.0% citrobacter sp., 4.0% enterococci, 0.5% staphylococcus aureus. results of sensitivity tests perform ... | 1989 | 2504948 |
| [susceptibility of clinical isolates to aztreonam]. | in vitro antibacterial activities of 9 antibiotics including aztreonam (azt) against clinically isolated gram-negative bacteria were determined using mic-2000 plus system. bacteria were isolated from clinical materials in saga medical school during a period from may 1987 to march 1988. summarized results were as follows: 1. azt showed excellent antibacterial activities against escherichia coli, klebsiella pneumoniae, proteus sp. and haemophilus influenzae, and mic80 values of azt against these o ... | 1989 | 2507799 |
| immunoprotective extracellular polysaccharides of klebsiella aerogenes capsular type k1, expressed in escherichia coli. | a gene library of genomic dna klebsiella aerogenes of capsular serotype k1 was constructed in e. coli le392 using the cosmid pmmb33. culture filtrates of e. coli recombinants were screened by elisa for extracellular polysaccharides specific for k. aerogenes k1. extracellular polysaccharide extracts from k. aerogenes k1 and 3% of the e. coli recombinants contained immunoprotective extracellular polysaccharides (iep) with similar chemical and immunological properties as shown by gel filtration thr ... | 1989 | 2517220 |
| human placental 17 beta-hydroxysteroid dehydrogenase is homologous to nodg protein of rhizobium meliloti. | the amino acid sequence of human placental 17 beta-hydroxysteroid dehydrogenase (17 beta-oh-steroid dehydrogenase) was found to be similar to that of the nodg protein of rhizobium meliloti. the computer-based comparison score is 11.5 sd higher than that obtained with 2500 comparisons of randomized sequences of these proteins. the probability of getting such a score by chance is 6 x 10(-31). 17 beta-oh-steroid dehydrogenase is also similar to klebsiella aerogenes ribitol dehydrogenase and escheri ... | 1989 | 2547159 |
| isolation and identification of 6-desmethylnaproxen sulfate as a new metabolite of naproxen in human plasma. | a new metabolite of naproxen, 6-desmethylnaproxen sulfate (6-dmns), has been identified in plasma from normal and uremic subjects after oral administration of a single 500 mg dose of naproxen. tentative identification was achieved by the finding of an increase in the concentration of 6-dmn upon incubation of the plasma with arylsulfatase from helix pomatia or from aerobacter aerogenes. more definitive identification was established through demonstration that the hplc retention time of the conjug ... | 1989 | 2566468 |
| survival of and plasmid stability in pseudomonas and klebsiella spp. introduced into agricultural drainage water. | cell survival and plasmid stability in pseudomonas fluorescens r2f and pseudomonas putida cym 318 containing respectively, plasmid rp4 and prk2501, and klebsiella aerogenes nctc 418 harboring plasmid pbr322 were studied in sterile and nonsterile agricultural drainage water under both aerobic and anaerobic conditions and in the absence and presence of added nutrients. both pseudomonas strains survived well in sterile drainage water incubated aerobically, with or without added nutrients. however, ... | 1989 | 2590305 |
| in-vitro activity of cefetamet (ro 15-8074) compared with other oral agents. | the bacteriostatic and bactericidal activities of cefetamet (ro 15-8074) were compared with those of cefadroxil, cefaclor, ampicillin, doxycycline, chloramphenicol and erythromycin against a total of 400 bacterial strains isolated from nosocomial respiratory tract and auditory canal infections. the broth macrodilution method was used to determine antimicrobial susceptibility. none of the compounds was effective against pseudomonas aeruginosa and methicillin-resistant staphylococcus aureus strain ... | 1989 | 2613603 |
| hydrogen evolution of enterobacter aerogenes depending on culture ph: mechanism of hydrogen evolution from nadh by means of membrane-bound hydrogenase. | the ph dependency of cell mass productivity, the hydrogen evolution rate and the yield of hydrogen from glucose was measured by controlling the ph of the culture automatically. the cell mass productivity of enterobacter aerogenes increased in a linear fashion up to a ph value of approx. 7.0. in contrast, both the evolution rate and the yield of hydrogen showed convex relationships up to a ph value of 7.0, both having maximum values at a ph of approx. 5.8. the maximum evolution rate was approx. 1 ... | 1989 | 2643990 |
| detection of antigens from gram-negative bacilli in urine of children with urinary tract infections. ii. thermostable bacterial proteins. | an enzyme immunoassay for detection of bacterial antigens in urine specimens from urinary tract infections (uti) was developed. the antigens detected in infections by enterobacteriaceae: escherichia coli, enterobacter aerogenes, and proteus sp. were different from the enterobacterial common antigen (eca). they were digested by trypsin and were characterized by remarkable thermostability; their molecular weights were approximately 34,000 and 31,000. they were detected by means of antisera to eca ... | 1989 | 2656535 |
| microcalorimetric studies of the effects of platinum group metal complexes on bacterial growth. | a range of platinum group metal complexes (pgmc) were screened for antibacterial activity against klebsiella aerogenes and staphylococcus aureus. the effects of the complexes were monitored by changes in the thermal and growth properties of the organisms. active complexes caused an immediate cessation of power and biomass production which did not recover whilst the complex was present in the medium. removal of the complex by washing the cells allowed the growth of the small viable proportion of ... | 1989 | 2661974 |
| [clinical studies on sulbactam/ampicillin in the field of pediatrics]. | during 8 months from october 1986 to may 1987, the clinical efficacy of sulbactam/ampicillin (sbt/abpc) was evaluated in 63 pediatric inpatients with various infections. clinical efficacies were evaluable in 58 patients among them (consisting of 2 patients with sepsis, 3 with tonsillitis, 12 with bronchitis, 6 with bronchopneumonia, 24 with pneumonia, 1 with phlegmon, 2 with lymphadenitis, 1 with impetigo and 7 with urinary tract infection) and were excellent in 40 patients and good in 17 with a ... | 1989 | 2664249 |
| [susceptibilities of clinical isolates to antibacterial agents. a study mainly focused on ofloxacin (the second report). reported by the research group for testing ofloxacin susceptibility on clinical isolates]. | susceptibilities of various clinical isolates to ofloxacin (oflx) and other antibacterial drugs were examined at 128 hospital laboratories in 36 prefectures throughout japan between april, 1986 and march, 1987. the results were totalized with an emphasis mainly on oflx and were compared with data obtained in the previous year. in this study, identification and susceptibility tests of the isolates were carried out at each hospital laboratory and the tests were performed according to the 1-dilutio ... | 1989 | 2664255 |
| "spontaneous" pneumocephalus associated with aerobic bacteremia. | three cases of "spontaneous" pneumocephalus suspected to have resulted from aerobic bacteremia caused by enterobacter cloacae, escherichia coli, and klebsiella aerogenes are reported. in two cases, the e. cloacae and k. aerogenes were isolated from the cerebrospinal fluid. these cases were characterized by a rapid accumulation of air, without niveau, in the subarachnoid space and ventricles. | 1989 | 2670144 |
| prevention of clinical coliform mastitis in dairy cows by a mutant escherichia coli vaccine. | a prospective cohort study was undertaken in two commercial california dairies. the treatment group, 246 cows, received three doses of a whole cell bacterin of j5 escherichia coli (mutant of e. coli o111:b4) plus freund's incomplete adjuvant vaccine (two in the dry period and one after calving) while 240 unvaccinated cows served as controls. thirty-five cases of clinical coliform mastitis were diagnosed, six in vaccinated cows and 29 in unvaccinated cows. bacteria isolated from the clinical case ... | 1989 | 2670166 |
| isolation and characterization of an alginate lyase from klebsiella aerogenes. | the bacterium klebsiella aerogenes (type 25) produced an inducible alginate lyase, whose major activity was located intracellularly during all growth phases. the enzyme was purified from the soluble fraction of sonicated cells by ammonium sulfate precipitation, anion- and cation-exchange chromatography and gel filtration. the apparent molecular weight of purified alginate lyase of 28,000 determined by gel filtration and of 31,600 determined by sodium dodecyl sulfate-polyacrylamide gel electropho ... | 1989 | 2673122 |
| competitive inhibitors of klebsiella aerogenes urease. mechanisms of interaction with the nickel active site. | we examined several compounds for their mechanisms of inhibition with the nickel-containing active site of homogeneous klebsiella aerogenes urease. thiolate anions competitively inhibit urease and directly interact with the metallocenter, as shown by the ph dependence of inhibition and by uv-visible absorbance spectroscopic studies. cysteamine, which possesses a cationic beta-amino group, exhibited a high affinity for urease (ki = 5 microm), whereas thiolates containing anionic carboxyl groups w ... | 1989 | 2674118 |
| comparison of sequences from the malb regions of salmonella typhimurium and enterobacter aerogenes with escherichia coli k12: a potential new regulatory site in the interoperonic region. | the male and malk genes from salmonella typhimurium, and the malefg operon and a portion of malk from enterobacter aerogenes were cloned and sequenced. plasmid-borne male genes from both species and the malf and malg genes from e. aerogenes were expressed normally in escherichia coli, and their products function in maltose transport. this shows that the malb products from the three species are interchangeable, at least in the combinations tested. the general genetic organization of the malb regi ... | 1989 | 2674653 |
| mutagenic dna repair genes on plasmids from the 'pre-antibiotic era'. | resistance transfer factors are natural conjugative plasmids encoding antibiotic resistance. some also encode mutagenic dna repair genes giving resistance to dna damage and induced mutagenesis. it has been shown that antibiotic resistance has been acquired by recent transposition events; however, we show here that mutagenic repair genes existed much earlier on these types of plasmids. conjugative plasmids from eight incompatibility groups from the murray collection of 'pre-antibiotic era' entero ... | 1989 | 2674658 |
| biosynthesis and secretion of pullulanase, a lipoprotein from klebsiella aerogenes. | we constructed, by site-directed mutagenesis, a mutant pullulanase gene in which the cysteine residue in a pentapeptide sequence, leu16-leu-ser-gly-cys20 within the nh2-terminal region of pullulanase from klebsiella aerogenes, is replaced by serine (ser20). the modification, processing, and subcellular localization of the mutant pullulanase were studied. labeling studies with [3h]palmitate and immunoprecipitation with mouse antiserum raised against pullulanase showed that the wild form of both t ... | 1989 | 2677008 |
| preservation of myocardial ultrastructure after 24 hours of klebsiella sepsis: histologic, functional, and biochemical correlations. | myocardial function with ultrastructure and high energy phosphate levels in dogs was correlated after 24 hours of sepsis using live klebsiella aerogenes. all animals developed progressive hemodynamic deterioration over a 24 hour period. mean arterial pressure decreased from 148 +/- 7 mmhg to 85 (p less than 0.01) and cardiac output decreased from 3.43 +/- .31 to 1.6 +/- 0.5 l/min. left ventricular stroke work decreased from 48.2 +/- 5 to 18.1 +/- 6 gm-meters (p less than 0.001). systemic and pul ... | 1989 | 2681798 |
| cadmium resistance in some members of enterobacteriaceae. | strains of members of enterobacteriaceae, namely escherichia coli (18), klebsiella aerogenes (16), and serratia marcescens (16) were screened for cd resistance or sensitivity. only one strain each of these was resistant to high levels (25 n moles/0.05 ml) cdcl2. the minimal inhibitory concentration (mic) of sensitive strains ranged from 0.8-5 micrograms/ml. all the resistant strains were simultaneously resistant to a number of antibiotics. treatment with sodium dodecyl sulfate eliminated resista ... | 1989 | 2684849 |
| ammonia-induced injury in pure cultures and natural populations of coliform bacteria. | ammonia-induced injury was investigated in pure cultures of escherichia coli and enterobacter aerogenes, and in natural coliform populations obtained from the oligotrophic luxapallila and the eutrophic sunflower rivers in northern mississippi. pure cultures were affected by ammonia exposure as indicated by changes in the injury ratio (ir) of cfu on m-t7 agar/cfu on m-endo agar. ammonia concentrations between 0 and 20 (mg nh3-n/1) had little or no effect and concentrations between 40 and 80 cause ... | 1989 | 2693200 |
| regulation of gene expression and cellular localization of cloned klebsiella aerogenes (k. pneumoniae) urease. | the genes for klebsiella aerogenes (k. pneumoniae) urease were cloned and the protein was overexpressed (up to 18% of total protein consisted of this enzyme) in several hosts. the small size of the dna encoding urease (3.5 kb), the restriction map, and the regulation of enzyme expression directed by the recombinant plasmid are distinct from other cloned ureases. nickel concentration did not affect urease gene expression, as demonstrated by the high levels of apoenzyme measured in cells grown in ... | 1989 | 2693604 |
| epidemiological typing of enterobacter aerogenes. | the applicability of enterobacter cloacae and klebsiella typing reagents for classifying clinical strains of enterobacter aerogenes was evaluated. of 75 strains, none were agglutinated by e. cloacae o antisera or were sensitive to e. cloacae bacteriophages. in contrast, 70 strains reacted with klebsiella capsular antisera. two-thirds of the strains were lysed by klebsiella typing phages. a set of five e. aerogenes bacteriocin producers classified 92% of strains into 15 sensitivity types. in conc ... | 1989 | 2715326 |
| comparative activity of newer antibiotics against gram-negative bacilli. | the in vitro activities of cefoperazone, cefotaxime, ceftriaxone, ceftazidime, azlocillin, mezlocillin, piperacillin, ticarcillin/clavulanate, aztreonam, imipenem, and ciprofloxacin were concurrently determined against over 1,000 isolates of gram-negative bacilli from clinical specimens of patients at the cleveland clinic. cephalosporins, penicillins, and aztreonam were active against species of enterobacteriaceae other than citrobacter freundii, enterobacter aerogenes, and enterobacter cloacae. ... | 1989 | 2731335 |
| formation of tyrosine o-sulfate by mitochondria and chloroplasts of euglena. | mitochondria that have been purified from cells of light-grown wild-type euglena gracilis klebs var. bacillaris cori or dark-grown mutant w10bsml and incubated with 35so4(2-) and atp accumulate a labeled compound in the surrounding medium. this compound is also labeled when mitochondria are incubated with [14c]tyrosine and nonradioactive sulfate under the same conditions. this compound shows exact coelectrophoresis with synthetic tyrosine o-sulfate at ph 2.0, 5.8, and 8.0, and yields sulfate and ... | 1989 | 2735764 |
| comparative in vitro activity of ciprofloxacin and other unrelated antimicrobials against bacterial respiratory tract pathogens. | ciprofloxacin is a new fluorinated 4-quinolone with a broad spectrum of antimicrobial activity which includes both gram-negative and gram-positive bacteria. in this study the in vitro activity of ciprofloxacin has been determined against bacteria associated with respiratory tract infections and compared with that of other antimicrobial agents used in the therapy of such infections. ciprofloxacin (mic90 0.008 mg/l) was highly active against haemophilus influenzae, including isolates producing bet ... | 1989 | 2737080 |
| a microbial carbon-phosphorus bond cleavage enzyme requires two protein components for activity. | enterobacter aerogenes ifo 12010 contains a carbon-phosphorus (c-p) bond cleavage enzyme catalyzing the liberation of inorganic phosphate from various alkyl- and phenylphosphonic acids. the enzyme in the bacterium was found to be composed of two physically different protein components, e2 and e3. the molecular weights of e2 and e3 were 560,000 and 110,000, respectively, and e3 was resolved into two apparently homogeneous subunits. neither component alone could catalyze the c-p bond cleavage reac ... | 1989 | 2753863 |
| isolation and survival of gentamicin resistant enterobacter aerogenes on finger tips of hospital personnel. | enterobacter aerogenes was isolated from the finger tips of 13.3 percent of hospital personnel while they were working in the wards. gentamicin resistant strains were isolated more frequently than gentamicin sensitive. carriage rate of e. aerogenes was higher among nurses than other staff members. there was no correlation between antibiotic resistance, capsular serotypes and survival of these strains on finger tips. the property of prolonged survival on finger tips is chromosomal in nature and n ... | 1989 | 2757748 |
| [meconium peritonitis complicated with enterobacter aerogenes infection--a case report]. | meconium peritonitis is an aseptic peritonitis that follows a perforation of the intestines in fetal life. it is a serious complication of intestinal perforation. this article reports a female newborn who was born with gestational age of 40 weeks and birth body weight of 3400 gm. she was admitted at age of 6 days, owing to persistent vomiting, abdominal distention, tachypnea and acrocyanosis since birth. the plain abdomen showed intra-abdominal calcification and intestinal distention. under the ... | 1989 | 2766063 |
| high efficiency transduction of single strand plasmid dna into enteric bacteria. | this report demonstrates high efficiency transduction of enteric bacteria using single strand plasmids packaged in m13 phage capsids. transformation by plasmid dna is usually a very inefficient process in many enteric bacteria other than escherichia coli k12. plasmids carrying an m13 origin of replication can be replicated and packaged when cells carrying such plasmids are infected with m13 or a derivative helper phage. by introducing an f' plasmid into e. coli, serratia marcescens, citrobacter ... | 1989 | 2779519 |
| origin of p-aminobenzoic acid from chorismic rather than iso-chorismic acid in enterobacter aerogenes and streptomyces species. | enzyme extracts from enterobacter aerogenes (62-1), streptomyces aminophilus, and streptomyces coelicolor were used to investigate the biosynthesis of p-aminobenzoic acid. the enzyme preparations from e. aerogenes and s. aminophilus contained both p-aminobenzoate synthase and iso-chorismate synthase activity, and were able to convert both chorismic and iso-chorismic acid to p-aminobenzoic acid. the apparent km for chorismic acid was, however, significantly lower than that for iso-chorismic acid, ... | 1989 | 2786373 |
| lipid a precursor from pseudomonas aeruginosa is completely acylated prior to addition of 3-deoxy-d-manno-octulosonate. | inhibition of lipopolysaccharide (lps) synthesis in pseudomonas aeruginosa at the stage of incorporation of 3-deoxy-d-manno-octulosonate (kdo) caused accumulation of a lipid a precursor which contained all of the fatty acids present on the lipid a of mature lps. the enzyme ctp:cmp-3-deoxy-d-manno-octulosonate cytidylyltransferase (cmp-kdo synthetase) from p. aeruginosa is inhibited by the kdo analog alpha-c-[1,5-anhydro-8-amino-2,7,8-trideoxy-d-manno-octopyranosyl] carboxylate (i), and i is effe ... | 1988 | 2833499 |
| bidirectional promoter in the hut(p) region of the histidine utilization (hut) operons from klebsiella aerogenes. | the hut(p) region (i.e., the region responsible for regulation of hutuh expression) of the klebsiella aerogenes histidine utilization (hut) operons contains a bidirectional promoter. one transcript from this promoter encodes the hutuh operon; the role of the oppositely directed transcript is unknown, although it appears to be involved in regulating hutuh expression (a.j. nieuwkoop, s.a. boylan, and r.a. bender, j. bacteriol. 159:934-939, 1984). a 247-base-pair (bp) fragment containing hut(p) car ... | 1988 | 2834335 |
| contact alters camp metabolism in aggregation-competent dictyostelium amoebae. | camp and cell-cell contact are involved in the coordination of differentiation and morphogenesis in dictyostelium discoideum. the experiments described in this paper establish a relationship between camp and cell-cell contact. contact between enterobacter aerogenes and aggregation-competent dictyostelium amoebae and contact between dictyostelium amoebae themselves results in the transient secretion of camp and an alteration in the amount of camp secreted in response to subsequent stimulation by ... | 1988 | 2854020 |
| plasmid-mediated conjugative transfer of klebsiella sp. rcs genesable to induce colanic acid capsular polysaccharide biosynthesis in escherichia coli. | regulation of capsular biosynthesis (rcs) genes, encoding the ability to induce the production of a colanic acid polysaccharide capsule, were transferred to escherichia coli by conjugation with klebsiella pneumoniae (aerogenes) of capsular serotype k36. transfer was mediated by a 58.4-mda conjugative plasmid of incompatibility group incm, which carried a copy of tn7 (specifying resistance to trimethoprim and streptomycin) together with determinants for several further resistances. this plasmid d ... | 1988 | 2856173 |
| pqq-linked enzymes in enteric bacteria. | klebsiella aerogenes possesses a pqq-linked glucose dehydrogenase. other members of the enterobacteriaceae, such as escherichia coli, salmonella typhimurium or serratia marcescens are seemingly unable to synthesize pqq, but are able to synthesize the glucose dehydrogenase apoenzyme. the physiological significance of this enzyme is discussed. | 1987 | 2856384 |
| fimbrial and non-fimbrial haemagglutinins in enterobacter aerogenes. | ten strains of enterobacter aerogenes were examined for their ability to produce haemagglutinins and fimbriae. nine strains formed a mannose-sensitive (ms) haemagglutinin associated with thin (4 nm) non-channelled fimbriae. these thin fimbriae of e. aerogenes were antigenically different from the thin fimbriae of other fimbriate strains of enterobacter and klebsiella and probably represent a new kind of fimbria not previously described in enterobacteriaceae. eight of these same nine strains also ... | 1985 | 2857201 |
| regulation of glutamine synthetase, aspartokinase, and total protein turnover in klebsiella aerogenes. | when suspensions of klebsiella aerogenes are incubated in a nitrogen-free medium there is a gradual decrease in the levels of acid-precipitable protein and of aspartokinase iii (lysine-sensitive) and aspartokinase i (threonine-sensitive) activities. in contrast, the level of glutamine synthetase increases slightly and then remains constant. under these conditions, the glutamine synthetase and other proteins continue to be synthesized as judged by the incorporation of [14c]leucine into the acid-p ... | 1985 | 2865982 |
| cloning and expression of the thiobacillus ferrooxidans glutamine synthetase gene in escherichia coli. | the glutamine synthetase (gs) gene glna of thiobacillus ferrooxidans was cloned on recombinant plasmid pmeb100 which enabled escherichia coli glna deletion mutants to utilize (nh4)2so4 as the sole source of nitrogen. high levels of gs-specific activity were obtained in the e. coli glna deletion mutants containing the t. ferrooxidans gs gene. the cloned t. ferrooxidans dna fragment containing the glna gene activated histidase activity in an e. coli glna glnl glng deletion mutant containing the kl ... | 1985 | 2866177 |
| isolation and identification of a new major metabolite of diflunisal in man. the sulfate conjugate. | a new metabolite of diflunisal has been identified in volunteers and patients after multiple dose administration. the metabolite was isolated from human urine by silica gel chromatography and was further purified by reversed phase hplc. arylsulfatase from helix pomatia and from aerobacter aerogenes completely hydrolyzed the isolated metabolite to diflunisal, although hydrolysis by bacterial arylsulfatase was extremely slow. electron impact mass spectra for diflunisal and its sulfate conjugate we ... | 1986 | 2868855 |
| the effects of sub-lethal concentrations of chlorhexidine on bacterial pathogenicity. | suspensions of escherichia coli and klebsiella aerogenes were exposed to low levels of chlorhexidine, which had little effect on their in vitro viability. a substantial reduction in their in vivo infectivity measured by intraperitoneal inoculation of mice was found. this selective reduction in bacterial infectivity by chlorhexidine should be taken into account when conventional in vitro tests are used to assess the clinical effectiveness of chlorhexidine-containing antiseptics. | 1986 | 2875100 |
| a study of the skin flora of spinal cord injured patients. | the skin flora of 11 spinally-injured patients was compared to that of 11 healthy control subjects. the perinea, groins, penile shafts and urethras of the patients were heavily colonized by a range of multi-drug resistant gram-negative bacilli. observations on patients from admission for up to 25 days suggest that the gram-negative bacilli start to colonize the skin 2-3 days after admission. some species, e.g., citrobacter diversus and escherichia coli appear as transient organisms while others ... | 1986 | 2876030 |
| anaerobes and septic abortion. | the bacterial flora of the genital tracts of twenty-two patients with septic abortion and twenty normal women (as controls) were studied to determine the significant aetiological agents. the predominant flora qualitatively and quantitatively in the twenty-two patients were anaerobes. bacteroides bivius was isolated from all the twenty-two patients and from twelve out of twenty normal controls. the next commonest anaerobes were b. asaccharolyticus (fourteen out of twenty-two patients), b. melanin ... | 1986 | 2876619 |
| a study of some factors associated with wound infection. | five hundred and four surgical patients were studied at princess basma hospital, north jordan, during the period april 1984-january 1985. the overall postoperative wound infection rate was 3.6%. in clean surgery 1.1% of wounds were infected and in potentially contaminated and contaminated surgery the infection rate was 3.3% and 18.5% respectively. major organisms isolated from infected wounds were staphylococcus aureus (55.5%), escherichia coli (16.6%), proteus vulgaris (11.1%), pseudomonas aeru ... | 1986 | 2878036 |
| an outbreak of serious klebsiella infections related to food blenders. | an investigation, including environmental sampling, was undertaken after four leukaemic patients on the same hospital ward developed serious infections with klebsiella aerogenes, capsular type k14. the source of this organism, common to all four patients, was found to be a food blender used for preparing milk-based drinks on the ward. | 1987 | 2883228 |
| expression and purification of glutamine synthetase cloned from bacteroides fragilis. | a glutamine synthetase (gs) gene, glna, from bacteroides fragilis was cloned on a recombinant plasmid pjs139 which enabled escherichia coli glna deletion mutants to utilize (nh4)2so4 as a sole source of nitrogen. dna homology was not detected between the b. fragilis glna gene and the e. coli glna gene. the cloned b fragilis glna gene was expressed from its own promoter and was subject to nitrogen repression in e. coli, but it was not able to activate histidase activity in an e. coli glna ntrb nt ... | 1986 | 2887626 |
| a laboratory model for testing agents for hygienic hand disinfection: handwashing and chlorhexidine for the removal of klebsiella. | a model for the laboratory assessment of hygienic hand disinfection is described which closely reproduces handwashing in a ward setting. the model uses a clinically relevant test organism and takes into account the natural antibacterial action of the skin. it also provides a means of measuring any residual antibacterial action after hand disinfection. using an epidemic multiply-resistant strain of klebsiella aerogenes k21, 0.5% chlorhexidine in iso-propyl alcohol yielded sterile post-disinfectio ... | 1988 | 2904456 |
| ribitol dehydrogenase of klebsiella aerogenes. sequence of the structural gene. | the ribitol dehydrogenase gene was cloned from wild-type klebsiella aerogenes and also from a transducing phage lambda prbt which expresses the rbt operon constitutively. the coding sequence for 249 amino acids is separated from the following d-ribulokinase gene by 31 base pairs containing three stop codons, one of which overlaps the ribosome binding site for d-ribulokinase. three residues in the amino acid sequence differ from that predicted from the dna sequence: asp-212 for asn-212 is probabl ... | 1985 | 2933028 |
| aztreonam in the treatment of bone and joint infections caused by gram-negative bacilli. | aztreonam was used successfully in 17 of 17 patients with orthopedic infections due to gram-negative bacilli (11, osteomyelitis; six, septic arthritis). duration of treatment ranged from 14 to 55 days, and the period of follow-up was four to 18 months. causative organisms included pseudomonas aeruginosa, serratia marcescens, enterobacter gergoviae, citrobacter diversus, proteus mirabilis, and enterobacter aerogenes. aztreonam was well tolerated. the only definite reactions attributable to aztreo ... | 1985 | 2934786 |
| in vitro activity of ciprofloxacin compared with other agents against recent hospital isolates. | ciprofloxacin's in vitro activity was tested against 385 hospital isolates originating from three geographically distinct regions. of all strains tested, only three (1 acinetobacter sp. and 2 pseudomonas aeruginosa) were ciprofloxacin resistant. ciprofloxacin was more active against escherichia coli, enterobacter cloacae, enterobacter aerogenes, acinetobacter sp., proteus sp., shigella sp. than gentamicin, mezlocillin and cefotaxime. it was more active than azlocillin and cefsulodin against p. a ... | 1986 | 2939966 |
| the klebsiella aerogenes glutamate dehydrogenase (gdha) gene: cloning, high-level expression and hybrid enzyme formation in escherichia coli. | the nadp-dependent glutamate dehydrogenase gene of klebsiella aerogenes was cloned in e. coli in the expression plasmid prk9. the cloned gene shows a high level of expression in e. coli in the hybrid plasmid pkg3 and such expression is independent of the vector promoter, as shown by experiments in which the promoter was deleted. active hybrid gdh hexamers were shown in cell-free extracts of an e. coli strain carrying cloned gdha genes of both e. coli and k. aerogenes. the nucleotide sequence of ... | 1985 | 2987645 |
| release of endotoxin from bacteria exposed to ciprofloxacin and its prevention with polymyxin b. | an in vitro system for studying the phenomenon of antibiotic-induced release of endotoxin from gram-negative bacteria is described. endotoxin was measured by a quantitative limulus lysate microassay. using a strain of escherichia coli, ciprofloxacin was shown to cause an immediate and sustained increase in endotoxin release compared to control cultures whereas gentamicin did not, despite an equally rapid bactericidal effect. the potential value of the anti-endotoxin effect of polymyxin b was exa ... | 1986 | 3009177 |
| sulbactam/ampicillin: in vitro spectrum, potency, and activity in models of acute infection. | more than 90% of community hospital-isolated strains of staphylococcus (including methicillin-resistant isolates), streptococcus, haemophilus, neisseria, branhamella, bacteroides, escherichia coli, klebsiella, enterobacter aerogenes, proteus, and acinetobacter calcoaceticus were inhibited by the sulbactam/ampicillin (1:2) combination at concentrations of 8 micrograms/16 micrograms per ml. the peak serum level from a 15-min infusion of 1 g/2 g of sulbactam/ampicillin is more than seven times this ... | 1986 | 3026001 |
| in vitro antimicrobial activity of diethyldithiocarbamate and dimethyldithiocarbamate against methicillin-resistant staphylococcus. | staphylococcus aureus has appeared which is highly resistant to both methicillin and aminoglycosides. current therapy involves long-term intravenous therapy of vancomycin. since vancomycin is currently the only drug used to treat these patients, there is a need to develop additional antimicrobial therapy. the in vitro antimicrobial effect of the metal chelator, diethyldithiocarbamate (ddtc) and its structural analog dimethyldithiocarbamate (dmtc) were investigated. both ddtc and dmtc were effect ... | 1987 | 3037985 |
| [use of imipenem-cilastatin in neonatal septicemias caused by gram-negative bacilli multiresistant to beta-lactam antibiotics]. | seven neonates with septicemia due to gram negative bacteria resistant to beta-lactam received imipenem-cilastatin therapy. bacteria isolated were enterobacter cloacae [3], enterobacter aerogenes [1], klebsiella pneumoniae [1], serratia marcescens [1], pseudomonas fluorescens [1]. the mics of imipenem were lower 1 microgram/ml. in 3 children septicemia occurred during previous antimicrobial chemotherapy. 3 iv 60 mg/kg doses of imipenem with amikacin (15 mg kg/d) were administered every day. for ... | 1988 | 3043351 |
| a bacterial factor induces changes in cysteine proteinase forms in the cellular slime mould dictyostelium discoideum. | the electrophoretic pattern of cysteine proteinases in axenically grown myxamoebae of dictyostelium discoideum can be altered by the addition of either gram-negative (klebsiella aerogenes, escherichia coli) or gram-positive (micrococcus lysodeikticus, bacillus subtilis) bacteria to the culture. no changes occurred, however, if either yeast or latex beads were used in place of bacteria. the changes involved the simultaneous loss of proteinases characteristic of the axenic cells (the a-forms) and ... | 1988 | 3052431 |
| divergence of the aerobactin iron uptake systems encoded by plasmids pcolv-k30 in escherichia coli k-12 and psmn1 in aerobacter aerogenes 62-1. | although the aerobactin-mediated iron uptake system has been characterized genetically in escherichia coli, the siderophore aerobactin was chemically characterized after purification from culture supernatants of aerobacter aerogenes 62-1, a member of the klebsielleae. we have cloned and mapped the genes encoding the aerobactin system genes of a. aerogenes 62-1 and begun characterization of the relevant proteins and enzymatic activities of this plasmid-mediated aerobactin system. published chemic ... | 1988 | 3053646 |
| evolution of aminobenzoate synthases: nucleotide sequences of salmonella typhimurium and klebsiella aerogenes pabb. | p-aminobenzoate synthase (ps) and anthranilate synthase (as) are structurally related enzymes that catalyze similar reactions and produce similar products, para- and ortho-aminobenzoate (anthranilate). each enzyme is composed of two non-identical subunits: a glutamine amidotransferase subunit (coii) and a subunit that produces an aminobenzoate product (coi). nucleotide sequence comparisons of the escherichia coli genes encoding each of the subunits suggest a common evolutionary origin for both s ... | 1988 | 3057324 |
| effect of subinhibitory concentration of gentamicin on adherence of klebsiella aerogenes to uroepithelial cells in vitro. | | 1988 | 3058587 |
| comparison of broth enrichment and direct plating for the isolation of campylobacter jejuni from dogs. | two techniques for the isolation of campylobacter jejuni from feces, direct plating and thioglycolate broth enrichment, were compared. a total of 272 rectal swab cultures were performed on 156 laboratory dogs. campylobacter blood agar plates and campylobacter thioglycolate broth were inoculated immediately upon sampling of the dogs. after incubation at 4 degrees c for 12 to 16 h, material from the campylobacter thioglycolate medium was inoculated onto campylobacter blood agar plates. a total of ... | 1988 | 3069857 |
| [a specific property of bacteria in the genus klebsiella--a color reaction with 5-aminosalicylic acid in the nutrient medium]. | for the first time bacteria of the genus klebsiella have been found to possess a specific property, characteristic of this genus only, i.e. the capacity of giving color reaction with 5-aminosalicylic acid. this reaction can be observed in all klebsiella species under study: k. pneumoniae (94.4 +/- 2.3%), k. ozaenae (93.3 +/- 4.5%), k. rhinoscleromatis (100%), k. oxytoca (88.0 +/- 4.9%), k. mobilis (92-5 +/- 4.3% of the strains). in all other bacteria under study (40 species, 22 genera and 7 fami ... | 1988 | 3072806 |
| [biochemical typing of enterobacter isolated from several clinical materials]. | a total of 74 enterobacter species have been isolated from the patients applying to the department of microbiology, university of ankara, studying several biochemical test systems their strains have been found. 37 of these enterobacter species have been found to be enterobacter cloacae, 10 enterobacter agglomerans, 13 enterobacter aerogenes, 3 enterobacter hafniae (hafnia alvei), 1 enterobacter sakazakii. 2 of the strains couldn't be classified. in conclusion most of the strains were found to be ... | 1988 | 3078792 |
| kinetic analysis of the reaction mechanism of oxaloacetate decarboxylase from klebsiella aerogenes. | the mechanism of oxaloacetate decarboxylase of klebsiella aerogenes was investigated by enzyme kinetic methods. the activity of the decarboxylase was strictly dependent on the presence of na+ or li+ ions. for li+ the km was about 17 times higher and the vmax about 4 times lower than for na+. no activity was detectable at na+ concentrations less than 5 microm. the curve for initial velocity versus na+ concentration was hyperbolic. initial velocity patterns with oxaloacetate or na+ as the varied s ... | 1986 | 3082631 |
| comparative study of serum bactericidal activity of cefotaxime alone or in combination with tobramycin. | the objectives of this study were to investigate the bactericidal activity in serum of cefotaxime alone or in combination with tobramycin against clinical strains and to determine the influence of tobramycin on the pharmacokinetics of cefotaxime. the peak bactericidal activity in serum of cefotaxime alone against klebsiella oxytoca, enterobacter aerogenes, serratia marcescens, pseudomonas cepacia, and listeria monocytogenes varied between 1:4 and 1:256. bactericidal activity could still be detec ... | 1986 | 3087282 |
| in vitro activity of aztreonam against gram negative bacteria from clinical specimens and its comparison with other commonly used antibiotics. | a total of 755 gram negative bacteria isolated from clinical specimens were tested against aztreonam by the disc agar diffusion test. the strains of bacteria used in this study consisted of escherichia coli (314), enterobacter aerogenes (30), e. agglomerans (7), e. cloacae, (39), citrobacter diversus (9), c. freundii (13), hafnia alvei (3), acinetobacter calcoaceticus (10), klebsiella oxytoca (6), k. ozaenae (5), k. pneumoniae (107), morganella morganii (3), moraxella sp. (10), pasteurella multo ... | 1986 | 3088346 |
| growth of salmonellas in different enrichment media. | the usefulness of selenite-f (s-f), tetrathionate (mkt) and rappaport-10 (r-10) broths as enrichment media to support growth of salmonellas either alone or in the presence of other competing organisms was studied. their ability to support the growth of stressed salmonellas from water was also investigated. it was observed that r-10 was more inhibitory to competing organisms than mkt and s-f. it strongly inhibited the growth of pseudomonas aeruginosa, citrobacter freundii and proteus vulgaris tho ... | 1986 | 3093439 |
| microcalorimetric studies on the effects of media and environmental conditions on the growth of bacteria. | a study has been made of the effects of media and oxygen tension on the power-time (p-t) traces exhibited by cells of klebsiella aerogenes, staphylococcus aureus and pseudomonas aeruginosa. the p-t traces were more complex in shape when the cells were grown in rich nutrient medium than when grown in chemically-defined glucose-limited medium. there was less waste heat and more biomass was produced during growth in nutrient broth. the oxygen tension had a profound effect on the shape of the p-t tr ... | 1986 | 3097461 |
| direct evidence for the participation of pyruvate in n-hydroxylation of lysine. | the contribution of pyruvate to the formation of n6-acetyl-n6-hydroxylysine by a cell-free system of aerobacter aerogenes 62-1 involved in the production of the dihydroxamate siderophore, aerobactin, has been assessed by a study of the influence of its analogs as well as of inhibitors of thiamine pyrophosphate-dependent decarboxylation reactions. these studies have provided unequivocal evidence for pyruvate functioning not only as a source of reducing equivalents in the initial step of n-hydroxy ... | 1987 | 3100331 |
| deletions in the tetracycline resistance determinant reduce the thermosensitivity of a trfa(ts) derivative of plasmid rp1 in pseudomonas aeruginosa. | a derivative of the broad-host-range plasmid rp1, pme301, was temperature-sensitive (ts) at 43 degrees c for maintenance in pseudomonas aeruginosa, p. mendocina, klebsiella aerogenes and escherichia coli. in e. coli, the ts defect of pme301 could be complemented in trans by the cloned trfa gene, which is known to be essential for rp1 replication in e. coli and p. aeruginosa. because pme301 expressed a ts phenotype in p. mendocina and k. aerogenes, we assume that the trfa function is also vital i ... | 1987 | 3111504 |
| purification and properties of pyridoxal-5'-phosphate-dependent histidine decarboxylases from klebsiella planticola and enterobacter aerogenes. | histidine decarboxylases from klebsiella planticola and enterobacter aerogenes were purified to homogeneity and compared with the histidine decarboxylase from morganella morganii. all three enzymes required pyridoxal 5'-phosphate as a coenzyme, showed optimal activity at ph 6.5, decarboxylated only histidine among the amino acids derived from protein, and were tetramers or dimers of identical subunits. amino-terminal sequences of the three enzymes showed up to 81% homology through residue 33, bu ... | 1987 | 3114230 |
| enumeration of pathogenic bacteria from sewage sludge in kuwait. | domestic sewage in kuwait is mainly treated by an activated sludge process. sewage sludges at different treatment stages and the digested sludge were studied for their content of pathogenic bacteria. two gram-positive cocci and eight gram-negative bacilli were isolated and enumerated in the sewage sludges and in raw sewage. during march, aeromonas hydrophila was the dominant bacterium isolated. serratia liquefaciens in raw sewage and sludge-2, and salmonella species in digested sludge were domin ... | 1987 | 3121985 |
| bacterial adhesion to intravenous cannulae: influence of implantation in the rabbit and of enzyme treatments. | comparison was made of the adhesion of staphylococcus epidermidis, staphylococcus aureus, escherichia coli, klebsiella aerogenes and pseudomonas aeruginosa to six types of intravascular cannula material. adhesion to materials removed from rabbit tissues did not differ significantly between types of material or between bacterial species. in contrast, major differences were found when unimplanted materials were examined; the overall rank order of adhesiveness of bacteria to unimplanted materials ( ... | 1988 | 3123262 |
| [pseudomonas aeruginosa and surgical intensive care units]. | the prevalence of pseudomonas aeruginosa in intensive care unit (icu) is 19 per cent although its incidence in blood culture is only 3.5 per cent and remains the same even though the total prevalence of this bacterium increases. a review of the clinical results shows that this germ causes severe complications in only 3 per cent of patients. a study by epidemiological markers reveals the presence of 16 different iats serotypes in the icu, this distribution of the serotypes being similar to this o ... | 1988 | 3132871 |
| in vitro evaluation of e1040, a new cephalosporin with potent antipseudomonal activity. | e1040 is a new parenteral cephalosporin with a broad antibacterial spectrum and potent antipseudomonal activity. the compound was four- to eightfold more active than ceftazidime and cefsulodin against pseudomonas aeruginosa (mic of e1040 for 90% of strains tested [mic90], 3.13 micrograms/ml). e1040 also showed a potent activity against other glucose-nonfermentative rods, including acinetobacter species. the activities of e1040 against most species of the family enterobacteriaceae were roughly co ... | 1988 | 3134847 |
| synthesis and structure-activity relationships in the cefpirome series. i. 7-[2-(2-aminothiazol-4-yl)-2-(z)-oxyiminoacetamido]-3-[(su bstituted-1-pyridinio)methyl]ceph-3-em-4-carboxylate s. | 7-[2-(2-aminothiazol-4-yl)-2-(z)-oxyiminoacetamido]-3-[(s ubs tituted-1-pyridinio)methyl]ceph-3-em-4-carboxylates ii are a group of beta-lactam antibiotics with extraordinary high antibacterial activity. the promising member of this group, cefpirome (hr 810, ii-1) is a candidate for clinical use. synthetic pathways to ii starting from cefotaxime derivatives i or 7-aminocephalosporanic acid (7-aca) are described. a preferred method for the conversion of i to ii or 7-aca to precursors iii respecti ... | 1988 | 3142844 |
| regulation of the galactose-inducible lac operon and the histidine utilization operons in pts mutants of klebsiella aerogenes. | galactose appears to be the physiological inducer of the chromosomal lac operon in klebsiella aerogenes. both lactose and galactose are poor inducers in strains having a functional galactose catabolism (gal) operon, but both are excellent inducers in gal mutants. thus the slow growth of k. aerogenes on lactose reflects the rapid degradation of the inducer. several pts mutations were characterized and shown to affect both inducer exclusion and permanent catabolite repression. the beta-galactosida ... | 1988 | 3142852 |
| in vitro activity of florphenicol. | florphenicol was active at a lower concentration than chloramphenicol against over half of 234 recent clinical bacterial isolates. the majority (98%) of the isolates were inhibited by florphenicol at a concentration of 8 mg/l or less. florphenicol was particularly effective against chloramphenicol resistant strains of haemophilus influenzae. klebsiella aerogenes and bacteroides spp. florphenicol was bacteristatic for salmonellae and escherichia coli but bactericidal for haemophilus influenzae. f ... | 1988 | 3143587 |
| [susceptibility of clinically isolated strains to aztreonam]. | in vitro antibacterial activities of aztreonam (azt) and cephems against clinically isolated 334 strains were investigated. the results obtained in the study are summarized as follows: 1. azt showed excellent antibacterial activities against clinically isolated 334 strains. 2. azt showed potent activities against escherichia coli, klebsiella pneumoniae, proteus spp., enterobacter aerogenes and citrobacter freundii. 3. antibacterial activities of azt were superior against enterobacter cloacae, se ... | 1988 | 3145344 |
| entire nucleotide sequence of the pullulanase gene of klebsiella aerogenes w70. | we determined the entire nucleotide sequence of the klebsiella aerogenes w70 pullulanase gene (pula) contained on a 4.2-kilobase-pair fragment of plasmid ppb174. the amino acid composition deduced from an open reading frame of 3,288 base pairs agreed closely with that determined for the intracellular pullalanase. the precursor enzyme consisted of 1,096 amino acid residues and contained a hydrophobic n-terminal signal peptide and the consensus sequence for the bacterial prelipoprotein signal pept ... | 1987 | 3155373 |
| [susceptibilities of clinical isolates to antibacterial agents. focusing mainly on ofloxacin (first report). reported by the research group for testing ofloxacin susceptibility of clinical isolates]. | susceptibility tests were carried out on a variety of clinically isolated pathogens using the susceptibility disc method at 197 hospitals in japan between may, 1985 through march, 1986. these tests were organized by the research group for testing ofloxacin susceptibility on clinical isolates, and the results were statistically analyzed. this paper describes a comparison of susceptibilities of clinical isolates including staphylococcus aureus, staphylococcus epidermidis, enterococcus faecalis, st ... | 1988 | 3163383 |
| rna polymerase as a repressor of transcription in the hut(p) region of mutant klebsiella aerogenes histidine utilization operons. | mutants of klebsiella aerogenes able to express the hutuh operon in the absence of positive effectors were isolated and characterized. these mutations improve the hutuh promoter (puh) by changing the -10 region to match the consensus sequence more closely. these mutations also affect another, oppositely oriented promoter in this region, pc. although the mutations lie far outside pc, they cause pc to be inactive, apparently because binding of rna polymerase to the puh promoter blocks the overlapp ... | 1988 | 3170491 |
| resistance studies with ofloxacin. | the selection of ofloxacin-resistant mutants from susceptible wild-type bacterial populations was investigated by three methods. resistant mutants selected from populations of escherichia coli (nctc 10418) and staphylococcus aureus (nctc 6571) by single-step passage at either four or eight times the mic occurred at a frequency of less than 1 x 10(-10). ofloxacin-resistant mutants of pseudomonas aeruginosa (nctc 10662), selected at four times the mic, occurred with a 100-fold greater frequency at ... | 1988 | 3182460 |
| detection of carbon-phosphorus lyase activity in cell free extracts of enterobacter aerogenes. | the bacterium enterobacter aerogenes could grow on a medium containing alkylphosphonic acid as a phosphorus source. the extracts prepared from the cells grown on phosphonoacetic acid as a sole source of phosphorus showed an activity of carbon-phosphorus lyase and hydrolyzed methyl-phosphonic acid, phosphonoacetic acid and phenylphosphonic acid with a liberation of inorganic phosphates. | 1988 | 3196331 |
| [clinical laboratory approach for estimating effective administrative dose of cefuzonam. evaluation of disc susceptibility test and its interpretation system]. | in vitro activities of cefuzonam (czon) against 273 clinical isolates were studied through the evaluation of mic's and the results of disc susceptibility test. the mic's were determined using the agar dilution method at an inoculum level of 10(6)cfu/ml. the mic80's of czon against streptococcus pneumoniae, escherichia coli, klebsiella pneumoniae, proteus mirabilis, proteus vulgaris, haemophilus influenzae and citrobacter spp. were less than 0.20 microgram/ml. the mic80's against serratia marcesc ... | 1988 | 3204654 |
| role of protozoa in microbial acclimation for mineralization of organic chemicals in sewage. | the role of protozoa in affecting the length of the acclimation period for the mineralization of organic compounds in sewage was investigated. the acclimation periods for the mineralization of 2 ng of p-nitrophenol, 100 ng of 2,4-dichlorophenoxyacetic acid, and 100 ng of 2,4-dichlorophenol per milliliter were markedly shortened or eliminated when the indigenous protozoa were inhibited with cycloheximide and nystatin. the extent of mineralization of the test compounds decreased when the protozoa ... | 1988 | 3208203 |
| [clinical laboratory approach for estimating effective administrative dose of sulbactam/cefoperazone]. | sulbactam/cefoperazone (sbt/cpz) is a preparation containing cpz and sbt, an inhibitor of beta-lactamases, at the ratio 1:1. the reliability of the sbt/cpz disc susceptibility test in estimating approximate values of mics and the utilization of the test in the evaluation of proper administrative doses were studied using 365 strains of clinical isolates. the antimicrobial activity of sbt/cpz was stronger than that of cpz alone. this increase in the antimicrobial activity due to the addition of sb ... | 1988 | 3210296 |
| [antibacterial activities of monobactams against fresh clinical isolates]. | antibacterial activities of monobactam antibiotics (carumonam (crmn) and aztreonam (azt] against gram-negative bacilli isolated from inpatients in the latter half of 1987 were investigated using penicillin (pc: piperacillin (pipc], cephems (ceps: ceftazidime (caz), cefotaxime (ctx), latamoxef (lmox), cefsulodin (cfs], carbapenem (imipenem (ipm] and pyridonecarboxylic acids (norfloxacin (nflx) and ofloxacin (oflx] as reference antibiotics. a total of 400 strains of 13 species, i.e. escherichia co ... | 1988 | 3210297 |
| [clinical laboratory approach in estimating the effective dosage of cefamandole]. | reliability of the cefamandole (cmd) disc susceptibility test in estimating approximate values of mics was studied using various clinical isolates totaling 246 strains with showa discs (8 mm diameter containing 30 micrograms of cmd). clinical significance of a 4 category system for the interpretation of the cmd disc tests, which is normally used in japan, was also evaluated to determine whether this system would be suitable or not for the evaluation of a proper dose of administration. the result ... | 1988 | 3221428 |
| [microbiological characteristics and detection of capsular forms of bacteria of the intestinal group in confectionery produced at the candy-chocolate factories]. | five types of confectionery and its semifinished products were investigated for contamination with klebsiella, mesophilic aerobic and elective anaerobic, coliform bacteria, e. coli, etc. after a long-term storage. e. coli and st. aureus were not detected after inoculation on 1 g of the product; mold fungi were identified only in singular samples, their level did not exceed 20 cfu/g; the level of mesophilic aerobic and elective anaerobic bacteria varied from several hundreds to 3000-5500 cfu/g; c ... | 1988 | 3232341 |
| in vitro susceptibility of bacteria to a ticarcillin-clavulanic acid combination. | in vitro testing of bacterial susceptibility to a combination of ticarcillin and clavulanic acid was done, using 406 aerobic gram-positive and gram-negative isolates (considered to be pathogens) cultured from equine and small animal specimens. a microdilution broth technique of susceptibility testing was performed, using trays with wells containing a range of doubling concentrations of dehydrated ticarcillin (range, 0.50 to 128 micrograms/ml) with fixed concentration of clavulanic acid (4 microg ... | 1988 | 3239838 |
| activity of cefixime (fk 027) for resistant gram-negative bacilli. | cefixime is an orally absorbable cephalosporin with an extended spectrum of in vitro activity for gram-negative bacteria especially members of the enterobacteriaceae. gram-negative rod isolates collected over a three month period were tested against cefixime. greater than 99% of escherichia coli isolates was susceptible to cefixime including those resistant to ampicillin. similarly isolates of klebsiella, proteus and providencia were also exquisitely susceptible to cefixime as were 91% of entero ... | 1988 | 3243091 |
| antibacterial activities of cefpodoxime, cefixime, and ceftriaxone. | cefpodoxime, cefixime, and ceftriaxone inhibited branhamella catarrhalis at less than or equal to 1 microgram/ml, beta-hemolytic streptococci at less than or equal to 0.25 microgram/ml, neisseria meningitidis at less than or equal to 0.06 microgram/ml, and haemophilus influenzae (other than beta-lactamase-negative, ampicillin-resistant isolates) at less than or equal to 0.12 microgram/ml. the mics for 50% of isolates of the family enterobacteriaceae other than citrobacter freundii, enterobacter ... | 1988 | 3245701 |
| clinical efficacy of carumonam. | carumonam is a new n-sulfo-beta-lactam antibiotic active against aerobic gram-negative bacteria. an open study was carried out to evaluate the efficacy, safety and tolerance of carumonam with either 1 g t.i.d. (group a) or 2 g t.i.d. (group b) in bacterial septicaemia or severe sepsis. a total of 24 patients (14 men and 10 women) were included in the study, their ages ranged from 48-87 years (mean age 59). eighteen patients were treated for bacteraemia, three for bronchopneumonia, two for urinar ... | 1988 | 3246211 |
| in vitro activity of an oral iminomethoxy aminothiazolyl cephalosporin, r-3746. | the in vitro activity of r-3746, an iminomethoxy aminothiazolyl cephalosporin with a ch2och3 moiety at position 3, was compared with those of other antibiotics. r-3746 inhibited the majority of hemolytic streptococci (groups a, b, c, f, and g) and streptococcus pneumoniae at less than 0.06 micrograms/ml, which was comparable to the activity of amoxicillin, 2- to 8-fold more active than cefixime, and 16- to 64-fold more active than cefaclor and cephalexin. ninety percent of beta-lactamase-produci ... | 1988 | 3260766 |
| eari, a restriction endonuclease from enterobacter aerogenes which recognizes 5'ctcttc3'. | | 1988 | 3263622 |
| nucleotide sequence and expression of the enterobacter aerogenes alpha-acetolactate decarboxylase gene in brewer's yeast. | the nucleotide sequence of a 1.4-kilobase dna fragment containing the alpha-acetolactate decarboxylase gene of enterobacter aerogenes was determined. the sequence contains an entire protein-coding region of 780 nucleotides which encodes an alpha-acetolactate decarboxylase of 260 amino acids. the dna sequence coding for alpha-acetolactate decarboxylase was placed under the control of the alcohol dehydrogenase i promoter of the yeast saccharomyces cerevisiae in a plasmid capable of autonomous repl ... | 1988 | 3278689 |
| tigemonam, an oral monobactam. | tigemonam is an orally administered monobactam. at less than or equal to 1 microgram/ml it inhibited the majority of strains of escherichia coli, klebsiella spp., enterobacter aerogenes, citrobacter diversus, proteus spp., providencia spp., aeromonas hydrophila, salmonella spp., shigella spp., serratia marcescens, and yersinia enterocolitica. at less than or equal to 0.25 microgram/ml it inhibited haemophilus spp., neisseria spp., and branhamella catarrhalis. it did not inhibit pseudomonas spp. ... | 1988 | 3279906 |
| the artificial heart: infection-related morbidity and its effect on transplantation. | between october, 1985, and september, 1987, a total of 195 patients received cardiac allografts and 15 candidates required mechanical support with the jarvik-7 total artificial heart. seven of the 15 died within 60 days of total artificial heart implant. there have been no late deaths, and survivors are unrestricted. six of 7 deaths were related to infection (mediastinitis, 5; pneumonia and sepsis, 1), and the remaining 1 was due to failure of the transplanted heart. respiratory tract infection ... | 1988 | 3281615 |