| [the effect of gamma-radiation and desiccation on the viability of the soil bacteria isolated from the alienated zone around the chernobyl nuclear power plant]. | methylobacterium extorquens, m. mesophilicum, and bacillus subtilis strains were found to be resistant to gamma-radiation, irrespective of whether they were isolated from the alienated zone around the chernobyl nuclear power plant or outside this zone. the ld90 of methylobacterium and b. subtilis strains with respect to gamma-radiation was 2.0-3.4 and 3.7-4.4 kgy, respectively, whereas their ld99.99 values were 4.5-6.9 and more than 10 kgy, respectively. the high threshold levels of gamma-radiat ... | 2002 | 12449639 |
| thermodynamics of the acid transition in blue copper proteins. | the thermodynamic parameters of the conformational transition occurring at low ph (acid transition, at) in blue copper proteins, involving protonation and detachment from the cu(i) ion of one histidine ligand, have been determined electrochemically for spinach and cucumber plastocyanins, rhus vernicifera stellacyanin, cucumber basic protein (cbp), and paracoccus versutus amicyanin. these data were obtained from direct protein electrochemistry experiments carried out at varying ph and temperature ... | 2002 | 12450394 |
| a methylobacterium-like organism from algal crusts covering silicone rubber electric insulators in africa. | the primary goals of this study were to isolate, identify and characterize culturable bacteria living in a close association with microalgae within green crusts covering silicone rubber electric insulators in tanzania. | 2002 | 12452957 |
| metal-ligand interactions in perturbed blue copper sites: a paramagnetic (1)h nmr study of co(ii)-pseudoazurin. | pseudoazurin is an electron transfer copper protein, a member of the cupredoxin family. the protein is frequently found in denitrifying bacteria, where it is the electron donor of nitrite reductase. the copper at the active site is coordinated to his40, cys78, his81 and met86 in a distorted tetragonal geometry. we have recorded and assigned the (1)h nmr spectra of co(ii)-substituted pseudoazurin from achromobacter cycloclastes. the (1)h nmr spectrum of co(ii)-pseudoazurin closely resembles that ... | 2003 | 12459901 |
| overexpression of a heterologous protein, haloalkane dehalogenase, in a poly-beta-hydroxybutyrate-deficient strain of the facultative methylotroph methylobacterium extorquens am1. | using an expression vector containing p(mxaf'), a strong native promoter, expression of a model heterologous protein, haloalkane dehalogenase, from xanthobacter autotrophicus gj10 was achieved in the methylotrophic bacterium, methylobacterium extorquens am1. although expression using the wild-type strain was <5% of total cell protein, expression at a level of 10% of the total cell protein was achieved in a mutant unable to synthesize poly-beta-hydroxybutyrate granules. two other tested heterolog ... | 2003 | 12474248 |
| chemically gated electron transfer. a means of accelerating and regulating rates of biological electron transfer. | long-range protein electron transfer [et] reactions may be relatively slow because of long et distance and low driving force. it is possible to dramatically increase the rate of such nonadiabatic reactions by using an adiabatic chemical reaction to activate the system for rapid et. three such examples are discussed; nitrogenase, pyruvate:ferredoxin oxidoreductase, and the methylamine dehydrogenase-amicyanin complex. in each example, the faster activated et reaction is gated (i.e., rate-limited) ... | 2002 | 12475211 |
| oleomonas sagaranensis gen. nov., sp. nov., represents a novel genus in the alpha-proteobacteria. | a gram-negative bacterium was previously isolated from an oil field in shizuoka, japan, and designated strain hd-1. here we have performed detailed characterization of the strain, and have found that it represents a novel genus. the 16s rrna sequence of strain hd-1 displayed highest similarity to various uncultured species (86.7-99.7%), along with 86.2-88.2% similarity to sequences from azospirillum, methylobacterium, rhizobium, and hyphomicrobium, all members of the alpha-proteobacteria. phylog ... | 2002 | 12480113 |
| novel methylotrophy genes of methylobacterium extorquens am1 identified by using transposon mutagenesis including a putative dihydromethanopterin reductase. | ten novel methylotrophy genes of the facultative methylotroph methylobacterium extorquens am1 were identified from a transposon mutagenesis screen. one of these genes encodes a product having identity with dihydrofolate reductase (dhfr). this mutant has a c(1)-defective and methanol-sensitive phenotype that has previously only been observed for strains defective in tetrahydromethanopterin (h(4)mpt)-dependent formaldehyde oxidation. these results suggest that this gene, dmra, may encode dihydrome ... | 2003 | 12511515 |
| qscr, a lysr-type transcriptional regulator and cbbr homolog, is involved in regulation of the serine cycle genes in methylobacterium extorquens am1. | a new gene, qscr, encoding a lysr-type transcriptional regulator that is a homolog of cbbr, has been characterized from the facultative methylotroph methylobacterium extorquens am1 and shown to be the major regulator of the serine cycle, the specific c1 assimilation pathway. the qscr mutant was shown to be unable to grow on c1 compounds, and it lacked the activity of serine-glyoxylate aminotransferase, a key enzyme of the serine cycle. activities of other serine cycle enzymes were decreased duri ... | 2003 | 12562792 |
| [microbial destruction of harmful organic contaminants inherent to atmosphere of living space]. | the investigation into the microbial destruction of harmful organic contaminants in the air of habitable rooms was performed with the assumption that microorganisms have labile metabolism and synthesize inducible enzymes involved in transformation of organics compounds. based on our observations, methylobacterium d-08 and arthrobacter pastens are able to synthesize inducible enzymes and transform ethanol, acetic acid, acetone, and aldehyde. association of these microorganisms does not reveal acu ... | 2002 | 12572127 |
| effects of engineering uphill electron transfer into the methylamine dehydrogenase-amicyanin-cytochrome c-551i complex. | within the methylamine dehydrogenase-amicyanin-cytochrome c-551i complex, electrons are transferred from tryptophan tryptophylquinone (ttq) to heme via the type i copper center of amicyanin. mutation of pro94 of amicyanin to phe increases the redox potential of the copper center within the protein complex by approximately 195 mv. this introduces a large energy barrier for the second electron transfer (et) step in this three-protein et chain. as a consequence of this mutation, the et rate from tt ... | 2003 | 12578392 |
| the tungsten-containing formate dehydrogenase from methylobacterium extorquens am1: purification and properties. | nad-dependent formate dehydrogenase (fdh1) was isolated from the alpha-proteobacterium methylobacterium extorquens am1 under oxic conditions. the enzyme was found to be a heterodimer of two subunits (alpha1beta1) of 107 and 61 kda, respectively. the purified enzyme contained per mol enzyme approximately 5 mol nonheme iron and acid-labile sulfur, 0.6 mol noncovalently bound fmn, and approximately 1.8 mol tungsten. the genes encoding the two subunits of fdh1 were identified on the m. extorquens am ... | 2003 | 12605683 |
| evidence for substrate activation of electron transfer from methylamine dehydrogenase to amicyanin. | electron transfer (et) from methylamine dehydrogenase (madh) to amicyanin may be true or gated et, depending upon the redox form of madh. et from the substrate-reduced aminoquinol form of madh is gated, and the reaction rate is dependent on the presence of monovalent cations. this et reaction has been studied in buffer free of monovalent cations. the reaction rate is orders of magnitude less than with saturating concentrations of monovalent cation. analysis of the temperature dependence of this ... | 2003 | 12630872 |
| reconstruction of c(3) and c(4) metabolism in methylobacterium extorquens am1 using transposon mutagenesis. | the growth of methylobacterium extorquens am1 on c(1) compounds has been well-studied, but little is known about how this methylotroph grows on multicarbon compounds. a tn5 transposon mutagenesis procedure was performed to identify genes involved in the growth of m. extorquens am1 on succinate and pyruvate. of the 15000 insertion colonies screened, 71 mutants were found that grew on methanol but either grew slowly or were unable to grow on one or both of the multicarbon substrates. for each of t ... | 2003 | 12634329 |
| applied waste-free recovery of methanol: a sustainable solution for chromatography laboratories. | in this paper, we present appliedmethanol recycling technology utilising chromatographic applications, which has been designed for an academic-size institution. the procedure is combined out of proper recovery technique and the biodegradation method intended for postprocessing residues. additionally, analytical methods controlling the quality of the process are described in detail in order to enable full transfer of the proposed methodology to the analogous institution. the recovered solvent is ... | 2002 | 12638746 |
| the role of the mxad protein in the respiratory chain of methylobacterium extorquens during growth on methanol. | the largest of the gene clusters coding for proteins involved in methanol oxidation is the cluster mxafjgir(s)ackldehb. disruption of most of these genes leads to lack of growth on methanol. the previous results showed that the mutant lacking mxad grows on methanol although at a low rate. this is explained by the low rate of methanol oxidation by whole cells. the specific activity of methanol dehydrogenase (mdh) is higher in the mutant but its electron acceptor (cytochrome c(l)) is unchanged. us ... | 2003 | 12686160 |
| promoters and transcripts for genes involved in methanol oxidation in methylobacterium extorquens am1. | twenty-five genes are involved in methanol oxidation to formaldehyde by the methanol dehydrogenase system in the facultative methylotroph methylobacterium extorquens am1 organized in five gene clusters. rt-pcr was used to assess the transcripts for the main gene clusters that encode methanol dehydrogenase and proteins required for its activity (mxafgjirsackldehb), and the enzymes that are required for the synthesis of the methanol dehydrogenase prosthetic group, pyrroloquinoline quinone (pqqabc/ ... | 2003 | 12686645 |
| loop-contraction mutagenesis of a type 1 copper site. | loop-contraction mutagenesis has been applied to the cupredoxin pseudoazurin to introduce the active-site loop of amicyanin. the mutation has a limited effect on the spectroscopic properties, and therefore structure, of the cupric protein. the loop contraction results in the increase of the pka for the detachable his ligand of pseudoazurin by two ph units, similar to the value as found in amicyanin. | 2003 | 12708836 |
| frozen density functional free energy simulations of redox proteins: computational studies of the reduction potential of plastocyanin and rusticyanin. | the evaluation of reduction potentials of proteins by ab initio approaches presents a major challenge for computational chemistry. this is addressed in the present investigation by reporting detailed calculations of the reduction potentials of the blue copper proteins plastocyanin and rusticyanin using the qm/mm all-atom frozen density functional theory, fdft, method. the relevant ab initio free energies are evaluated by using a classical reference potential. this approach appears to provide a g ... | 2003 | 12708852 |
| microvirga subterranea gen. nov., sp. nov., a moderate thermophile from a deep subsurface australian thermal aquifer. | a strictly aerobic bacterium, strain fail4t, was isolated from free-flowing geothermal waters of a bore (bore register no. 3768) tapping the great artesian basin of australia. the non-sporulating, gram-negative cells of strain fail4t produced light-pink colonies, were rod-shaped (1 x 1.5-4 microm) and were motile by a single polar flagellum. strain fail4t grew optimally at 41 degrees c at a ph of 7.0 and had an absolute requirement for yeast extract. the strain grew on casein hydrolysate, trypto ... | 2003 | 12710604 |
| methylotrophy in methylobacterium extorquens am1 from a genomic point of view. | | 2003 | 12730156 |
| construction of biofilms with defined internal architecture using dielectrophoresis and flocculation. | a novel approach was developed for the construction of biofilms with defined internal architecture using ac electrokinetics and flocculation. artificial structured microbial consortia (asmc) consisting of localized layered microcolonies of different cell types were formed by sequentially attracting different cell types to high field regions near microelectrodes using dielectrophoresis. stabilization of the microbial consortia on the electrode surface was achieved by crosslinking the cells using ... | 2003 | 12740931 |
| [the biology of aerobic methylobacteria capable of degrading halomethanes]. | recent data on the biology of aerobic methylotrophic bacteria capable of utilizing toxic halogenated methane derivatives as sources of carbon and energy are reviewed, with particular emphasis on the taxonomic, physiological, and biochemical diversity of mono- and dihalomethane-degrading methylobacteria and the enzymatic and genetic aspects of their primary metabolism. the initial steps of chloromethane dehalogenation to formate and hcl through a methylated corrinoid and methyletrahydrofolate are ... | 2003 | 12751236 |
| monitoring of unfolding of metallo-proteins by electrospray ionization mass spectrometry. | an electrospray ionisation (esi) mass spectrometric method for the determination of the equilibrium constant and free energy (deltag) of protein unfolding was used to monitor the denaturation process at different ph of three metallo-proteins, i.e. wild-type copper azurin, zinc azurin and wild-type amicyanin. the time course of the unfolding process was followed by dissolving the proteins under denaturing conditions (methanol-water (1 : 1, v/v)) at different ph (2.5, 3.0, 3.5) and recording esi s ... | 2003 | 12794870 |
| novel thermo-acidophilic bacteria isolated from geothermal sites in yellowstone national park: physiological and phylogenetic characteristics. | moderately thermophilic acidophilic bacteria were isolated from geothermal (30-83 degrees c) acidic (ph 2.7-3.7) sites in yellowstone national park. the temperature maxima and ph minima of the isolates ranged from 50 to 65 degrees c, and ph 1.0-1.9. eight of the bacteria were able to catalyze the dissimilatory oxidation of ferrous iron, and eleven could reduce ferric iron to ferrous iron in anaerobic cultures. several of the isolates could also oxidize tetrathionate. six of the iron-oxidizing is ... | 2003 | 12802481 |
| bacterial degradation of dichloromethane in cultures and natural environments. | dichloromethane (dcm) is a toxic pollutant showing prolonged persistence in water. dcm biodegradation is usually determined from increases in cl ions, gas chromatography, or by using radioisotopes. herein, we present an original and easy spectrophotometric method to estimate dcm concentrations in cultures and environmental samples during dcm biodegradation experiments. | 2003 | 12842489 |
| control of metalloprotein reduction potential: compensation phenomena in the reduction thermodynamics of blue copper proteins. | the reduction thermodynamics (delta h degrees '(rc) and delta s degrees '(rc)) for native paracoccus versutus amicyanin, for alcaligenes faecalis s-6 pseudoazurin, and for the g45p, m64e, and k27c variants of pseudomonas aeruginosa azurin were measured electrochemically. comparison with the data available for other native and mutated blue copper proteins indicates that the features of metal coordination and the electrostatic potential due to the protein matrix and the solvent control the reducti ... | 2003 | 12885256 |
| [the fractionation of chlorine isotopes by the aerobic methylotrophic bacterium methylobacterium dichloromethanicum grown on dichloromethane]. | methylobacterium dichloromethanicum was found to be able to utilize dichloromethane (dcm) as the source of carbon and energy with the production of biomass, co2, and hcl. a comparative analysis of abundances of the major dcm isotopomers 35cl(2)12c1h2, 35cl37cl12c1h2 and 37cl(2)12ch2 made it possible to estimate the fractionation of chlorine isotopes during the bacterial metabolism of dcm. the kinetic chlorine isotope effects for 35cl37cl12c1h2 (m/z 86) and 37cl(2)12c1h2 (m/z 88) relative to 35cl ... | 2003 | 12901015 |
| isolation of microorganisms for biological detoxification of lignocellulosic hydrolysates. | acid pretreatment of lignocellulosic biomass releases furan and phenolic compounds, which are toxic to microorganisms used for subsequent fermentation. in this study, we isolated new microorganisms for depletion of inhibitors in lignocellulosic acid hydrolysates. a sequential enrichment strategy was used to isolate microorganisms from soil. selection was carried out in a defined mineral medium containing a mixture of ferulic acid (5 mm), 5-hydroxymethylfurfural (5-hmf, 15 mm), and furfural (20 m ... | 2004 | 12908085 |
| quantification of central metabolic fluxes in the facultative methylotroph methylobacterium extorquens am1 using 13c-label tracing and mass spectrometry. | the metabolic fluxes of central carbon metabolism were measured in chemostat-grown cultures of methylobacterium extorquens am1 with methanol as the sole organic carbon and energy source and growth-limiting substrate. label tracing experiments were carried out using 70% (13)c-methanol in the feed, and the steady-state mass isotopomer distributions of amino acids derived from total cell protein were measured by gas chromatography coupled to mass spectrometry. fluxes were calculated from the isotop ... | 2003 | 12910542 |
| methylobacterium sp. isolated from a finnish paper machine produces highly pyruvated galactan exopolysaccharide. | the slime-forming bacterium methylobacterium sp. was isolated from a finnish paper machine and its exopolysaccharide (eps) was produced on laboratory scale. sugar compositional analysis revealed a 100% galactan (eps). however, ft-ir showed a very strong peak at 1611 cm(-1) showing the presence of pyruvate. analysis of the pyruvate content revealed that, based on the sugar composition, the eps consists of a trisaccharide repeating unit consisting of d-galactopyranose and [4,6-o-(1-carboxyethylide ... | 2003 | 12932368 |
| changing concepts in the systematics of bacterial nitrogen-fixing legume symbionts. | as of february 2003, bacteria that form nitrogen-fixing symbiotic associations with legumes have been confirmed in 44 species of 12 genera. phylogenies of these taxa containing legume symbionts based on the comparative analysis of 16s rdna sequences show that they are not clustered in one lineage but are distributed in the classes alphaproteobacteria and betaproteobacteria, and dispersed over the following nine monophyletic groups, being intermingled with other taxa that do not contain legume sy ... | 2003 | 12949698 |
| an engineered cua amicyanin capable of intermolecular electron transfer reactions. | the type i copper center of amicyanin was replaced with a binuclear cua center. to create this model cua protein, a portion of the amino acid sequence that contains three of the ligands to the native type i copper center of paracoccus denitrificans amicyanin was replaced with the corresponding portion of sequence that provides five ligands for the cua center of cytochrome c oxidase from p. denitrificans. uv-visible and electron paramagnetic resonance spectroscopy confirm that the engineered prot ... | 2003 | 12970350 |
| bosea minatitlanensis sp. nov., a strictly aerobic bacterium isolated from an anaerobic digester. | a strictly aerobic, mesophilic bacterium, strain amx 51(t), was isolated from anaerobic digester sludge. cells were gram-negative, motile, non-sporulating, straight to curved rods with one polar flagellum. the isolate had phenotypic traits of the genus bosea, including cellular fatty acid and substrate utilization profiles. physiological characteristics and antibiotic susceptibility were determined. phylogenetic analysis revealed that strain amx 51(t) was a member of the alpha-proteobacteria, mo ... | 2003 | 13130002 |
| temporal change in culturable phenanthrene degraders in response to long-term exposure to phenanthrene in a soil column system. | widespread environmental contamination by polycyclic aromatic hydrocarbons (pah) has led to increased interest in the use of natural attenuation as a clean-up strategy. however, few bioremediation studies have investigated the behaviour of the indigenous pah-degrading community after long-term exposure to a pah. in this study, a column packed with sandy loam soil was exposed to a solution saturated with phenanthrene ( approximately 1.2 mg l-1) for a 6-month period to examine the temporal respons ... | 2003 | 14510842 |
| [specificity of bacterial response to variation of isotopic composition of water]. | culture growth of bacteria with different membrane lipid composition was studied in liquid media with different isotope content of water. the concentration of deuterium in water was varied from 0.01 to 90%. it was shown that large concentrations of deuterium cause the inhibition of all cultures except deinococcus radiodurans, the most stable living cell. two cultures, methylobacterium organophilum and hyphomonas jannaschiana, showed a pronounced activation at a deuterium concentration of 0.01%. ... | 2003 | 14515487 |
| the significance of the flexible loop in the azurin (az-iso2) from the obligate methylotroph methylomonas sp. strain j. | the obligate methylotroph methylomonas sp. strain j produces two azurins (az-iso1 and az-iso2) as candidates for electron acceptor from methylamine dehydrogenase (madh) in the electron-transfer process involving the oxidation of methylamine to formaldehyde and ammonia. the x-ray crystallographic study indicated that az-iso2 gives two types of crystals (form i and form ii) with polyethylene glycol (peg4000) and ammonium sulfate as the precipitants, respectively. comparison between the two az-iso2 ... | 2003 | 14516747 |
| formaldehyde-detoxifying role of the tetrahydromethanopterin-linked pathway in methylobacterium extorquens am1. | the facultative methylotroph methylobacterium extorquens am1 possesses two pterin-dependent pathways for c(1) transfer between formaldehyde and formate, the tetrahydrofolate (h(4)f)-linked pathway and the tetrahydromethanopterin (h(4)mpt)-linked pathway. both pathways are required for growth on c(1) substrates; however, mutants defective for the h(4)mpt pathway reveal a unique phenotype of being inhibited by methanol during growth on multicarbon compounds such as succinate. it has been previousl ... | 2003 | 14645276 |
| purification of the formate-tetrahydrofolate ligase from methylobacterium extorquens am1 and demonstration of its requirement for methylotrophic growth. | the serine cycle methylotroph methylobacterium extorquens am1 contains two pterin-dependent pathways for c(1) transfers, the tetrahydrofolate (h(4)f) pathway and the tetrahydromethanopterin (h(4)mpt) pathway, and both are required for growth on c(1) compounds. with the exception of formate-tetrahydrofolate ligase (ftfl, alternatively termed formyl-h(4)f synthetase), all of the genes encoding the enzymes comprising these two pathways have been identified, and the corresponding gene products have ... | 2003 | 14645277 |
| isolation and 16s dna characterization of soil microorganisms from tropical soils capable of utilizing the herbicides hexazinone and tebuthiuron. | six non-fermentative bacteria were isolated from colombian (south america) and hawaiian (usa) soils after enrichment with minimal medium supplemented with two herbicides, hexazinone (hex) and tebuthiuron (teb). microscopic examination and physiological tests were followed by partial 16s dna sequence analysis, using the first 527 bp of the 16s rrna gene for bacterial identification. the isolated microorganisms (and in brackets, the herbicide that each degraded) were identified as: from colombia. ... | 2003 | 14649709 |
| genetic characterization of the carotenoid biosynthetic pathway in methylobacterium extorquens am1 and isolation of a colorless mutant. | genomic searches were used to reconstruct the putative carotenoid biosynthesis pathway in the pink-pigmented facultative methylotroph methylobacterium extorquens am1. four genes for putative phytoene desaturases were identified. a colorless mutant was obtained by transposon mutagenesis, and the insertion was shown to be in one of the putative phytoene desaturase genes. mutations in the other three did not affect color. the tetracycline marker was removed from the original transposon mutant, resu ... | 2003 | 14660416 |
| multiple formate dehydrogenase enzymes in the facultative methylotroph methylobacterium extorquens am1 are dispensable for growth on methanol. | formate dehydrogenase has traditionally been assumed to play an essential role in energy generation during growth on c(1) compounds. however, this assumption has not yet been experimentally tested in methylotrophic bacteria. in this study, a whole-genome analysis approach was used to identify three different formate dehydrogenase systems in the facultative methylotroph methylobacterium extorquens am1 whose expression is affected by either molybdenum or tungsten. a complete set of single, double, ... | 2004 | 14679220 |
| enhanced sensitivity of dna- and rrna-based stable isotope probing by fractionation and quantitative analysis of isopycnic centrifugation gradients. | stable isotope probing (sip) of nucleic acids allows the detection and identification of active members of natural microbial populations that are involved in the assimilation of an isotopically labelled compound into nucleic acids. sip is based on the separation of isotopically labelled dna or rrna by isopycnic density gradient centrifugation. we have developed a highly sensitive protocol for the detection of 'light' and 'heavy' nucleic acids in fractions of centrifugation gradients. it involves ... | 2004 | 14686943 |
| full matrix refinement as a tool to discover the quality of a refined structure. | | 2003 | 14696381 |
| development of an insertional expression vector system for methylobacterium extorquens am1 and generation of null mutants lacking mtda and/or fch. | over the past few years, the genetic 'toolkit' available for use with methylobacterium extorquens am1 has expanded significantly. here a further advance is presented and demonstrated, an insertional expression system that allows expression of genes from a stable, unmarked chromosomal locus. this system has been used to better understand the role of the tetrahydrofolate (h4f) pathway in methylotrophy. previously, it has not been possible to generate null mutants lacking either mtda (encoding an n ... | 2004 | 14702393 |
| biodegradation of nitro-substituted explosives 2,4,6-trinitrotoluene, hexahydro-1,3,5-trinitro-1,3,5-triazine, and octahydro-1,3,5,7-tetranitro-1,3,5-tetrazocine by a phytosymbiotic methylobacterium sp. associated with poplar tissues (populus deltoides x nigra dn34). | a pink-pigmented symbiotic bacterium was isolated from hybrid poplar tissues (populus deltoides x nigra dn34). the bacterium was identified by 16s and 16s-23s intergenic spacer ribosomal dna analysis as a methylobacterium sp. (strain bj001). the isolated bacterium was able to use methanol as the sole source of carbon and energy, which is a specific attribute of the genus methylobacterium. the bacterium in pure culture was shown to degrade the toxic explosives 2,4,6-trinitrotoluene (tnt), hexahyd ... | 2004 | 14711682 |
| meab is a component of the methylmalonyl-coa mutase complex required for protection of the enzyme from inactivation. | adenosylcobalamin-dependent methylmalonyl-coa mutase catalyzes the interconversion of methylmalonyl-coa and succinyl-coa. in humans, deficiencies in the mutase lead to methylmalonic aciduria, a rare disease that is fatal in the first year of life. such inherited deficiencies can result from mutations in the mutase structural gene or from mutations that impair the acquisition of cobalamins. recently, a human gene of unknown function, mmaa, has been implicated in methylmalonic aciduria (dobson, c. ... | 2004 | 14734568 |
| fast, long-range electron-transfer reactions of a "blue" copper protein coupled non-covalently to an electrode through a stilbenyl thiolate monolayer. | a self-assembled monolayer (sam), formed by the insitu saponification of a stilbenyl thioacetate on a gold electrode, yields fast electron transfer (et)(the exchange rate at zero driving force exceeds 1600 s-1) with adsorbed molecules of the blue copper protein, azurin, over a distance exceeding 15 angstroms . | 2004 | 14740055 |
| rare bacterium of new genus isolated with prolonged enrichment culture. | dynamic change in microbial flora was monitored with an oxygen electrode. the 1st phase microorganisms, which first grew well in lb medium, were followed by the 2nd phase microorganisms, which supposedly assimilated microbial cells of the 1st phase and their metabolites. in a similar way, a change in microbial flora was observed from the 1st phase to the 4th phase in 84 hr. based on this observation, prolonged enrichment culture was done for as long as two months to increase the ratio of existen ... | 2004 | 14745160 |
| microbial characterization during the early habitation of the international space station. | an evaluation of the microbiota from air, water, and surface samples provided a baseline of microbial characterization onboard the international space station (iss) to gain insight into bacterial and fungal contamination during the initial stages of construction and habitation. using 16s genetic sequencing and rep-pcr, 63 bacterial strains were isolated for identification and fingerprinted for microbial tracking. of the bacterial strains that were isolated and fingerprinted, 19 displayed similar ... | 2004 | 14749908 |
| temporal dynamics and degradation activity of an bacterial inoculum for treating waste metal-working fluid. | in order for established bioreactors to be effective for treating chemically mixed wastes such as metal working fluids (mwf) it is essential that they harbour microbial populations that can maintain sufficient active biomass and degrade each of the chemical constituents present. in this study we investigated the effectiveness of a bacterial consortium composed of four species (clavibacter michiganensis, methylobacterium mesophilicum, rhodococcus erythropolis and pseudomonas putida), assembled on ... | 2004 | 14871209 |
| characterization of two methanopterin biosynthesis mutants of methylobacterium extorquens am1 by use of a tetrahydromethanopterin bioassay. | an enzymatic assay was developed to measure tetrahydromethanopterin (h(4)mpt) levels in wild-type and mutant cells of methylobacterium extorquens am1. h(4)mpt was detectable in wild-type cells but not in strains with a mutation of either the orf4 or the dmra gene, suggesting a role for these two genes in h(4)mpt biosynthesis. the protein encoded by orf4 catalyzed the reaction of ribofuranosylaminobenzene 5'-phosphate synthase, the first committed step of h(4)mpt biosynthesis. these results provi ... | 2004 | 14973120 |
| production of heterologous protein by methylobacterium extorquens in high cell density fermentation. | the green fluorescent protein (gfp) was used as a model protein to study the recombinant protein production by the strain methylobacterium extorquens atcc 55366. scale-up from shake flasks to 20 l fed-batch fermentation was achieved using methanol as a sole carbon and energy source and a completely minimal culture medium. two different expression vectors were used to express gfp. clone pcm-gfp containing the vector pcm110 with native promoter of the methanol dehydrogenase pmxaf produced approxim ... | 2004 | 14987765 |
| biochemical characterization of a dihydromethanopterin reductase involved in tetrahydromethanopterin biosynthesis in methylobacterium extorquens am1. | during growth on one-carbon (c1) compounds, the aerobic alpha-proteobacterium methylobacterium extorquens am1 synthesizes the tetrahydromethanopterin (h4mpt) derivative dephospho-h4mpt as a c1 carrier in addition to tetrahydrofolate. the enzymes involved in dephospho-h4mpt biosynthesis have not been identified in bacteria. in archaea, the final step in the proposed pathway of h4mpt biosynthesis is the reduction of dihydromethanopterin (h2mpt) to h4mpt, a reaction analogous to the reaction of the ... | 2004 | 15028691 |
| balneomonas flocculans gen. nov., sp. nov., a new cellulose-producing member of the alpha-2 subclass of proteobacteria. | a new bacterial strain capable of producing cellulose was isolated from a hot spring. the isolate was gram-negative, aerobic, and rod-shaped. the optimum temperature for growth was 40-45 degrees c. methanol, glucose and other common carbohydrates were not utilized as sole growth substrates. thiosulfate was not oxidized. the g+c content of the dna was determined to be 64.0 mol%. comparative 16s rdna analysis indicated that bosea thiooxidans and some strains of the genus methylobacterium were the ... | 2004 | 15046301 |
| [physiological and biochemical analysis of the transformants of aerobic methylobacteria expressing the dcm a gene of dichloromethane dehydrogenase]. | the transformants of methylobacterium dichloromethanicum dm4 (dm4-2cr-/pme8220 and dm4-2cr-/pme8221) and of methylobacterium extorquens am1 (am1/pme8220 and am1/pme8221) that express the dcm a gene of dichloromethane dehalogenase undergo lysis when incubated in the presence of dichloromethane and are sensitive to acidic shock. the lysis of the transformants was found to be related neither to the accumulation of cl- ions, ch2o, and hcooh, nor to the impairment of glutathione synthesis or to the m ... | 2004 | 15074037 |
| [effect of uv-radiation and drying on bacterium diversity in soil]. | it has been shown that after dna-injuring factors (uv irradiation or drying) action on soil one could observe the decrease of the total quantity of bacteria and the number of species, i.e., the decrease of microbe diversity. at the same time not numerous species were found in soils after their action. thus the drying or uv-irradiation makes it possible to estimate more completely the microbe diversity in soils as well as to find resistant bacteria. it has been established that the strain methylo ... | 2004 | 15104058 |
| amoebae-resisting bacteria isolated from human nasal swabs by amoebal coculture. | amoebae feed on bacteria, and few bacteria can resist their microbicidal ability. amoebal coculture could therefore be used to selectively grow these amoebae-resisting bacteria (arb), which may be human pathogens. to isolate new arb, we performed amoebal coculture from 444 nasal samples. we recovered 7 (1.6%) arb from 444 nasal swabs, including 4 new species provisionally named candidatus roseomonas massiliae, c. rhizobium massiliae, c. chryseobacterium massiliae, and c. amoebinatus massiliae. t ... | 2004 | 15109415 |
| bacterial communities associated with flowering plants of the ni hyperaccumulator thlaspi goesingense. | thlaspi goesingense is able to hyperaccumulate extremely high concentrations of ni when grown in ultramafic soils. recently it has been shown that rhizosphere bacteria may increase the heavy metal concentrations in hyperaccumulator plants significantly, whereas the role of endophytes has not been investigated yet. in this study the rhizosphere and shoot-associated (endophytic) bacteria colonizing t. goesingense were characterized in detail by using both cultivation and cultivation-independent te ... | 2004 | 15128517 |
| interpretation of the temperature-dependent color of blue copper protein mutants. | the electronic absorption spectrum of the mutant of the blue copper protein amicyanin with a pseudoazurin loop (amipse) shows a remarkable temperature dependence. the absorption band at approximately 460 nm increases at low temperature while the transition at approximately 600 nm is not much affected by a variation of the temperature. an approximate density functional theory (dft) study of the active site model [cu(ii)(imidazole)(2)(sch(3))(s(ch(3))(2))](+) (protein backbone and solvation neglec ... | 2004 | 15134935 |
| bud endophytes of scots pine produce adenine derivatives and other compounds that affect morphology and mitigate browning of callus cultures. | endophytes are found in meristematic bud tissues of scots pine (pinus sylvestris l.) especially prior to growth, which would suggest their involvement in growth of the bud. to test this hypothesis, production of phytohormones by two bacterial (methylobacterium extorquens, pseudomonas synxantha) and one fungal endophyte (rhodotorula minuta) was studied by mass spectrometry. the most common gibberellins, auxins, or cytokinins were not detected in the fractions studied. instead, m. extorquens and r ... | 2004 | 15153198 |
| characterization and heterologous gene expression of a novel esterase from lactobacillus casei cl96. | a novel esterase gene (esti) of lactobacillus casei cl96 was localized on a 3.3-kb bamhi dna fragment containing an open reading frame (orf) of 1,800 bp. the orf of esti was isolated by pcr and expressed in escherichia coli, the methylotrophic bacterium methylobacterium extorquens, and the methylotrophic yeast pichia pastoris under the control of t7, methanol dehydrogenase (p(mxaf)), and alcohol oxidase (aox1) promoters, respectively. the amino acid sequence of esti indicated that the esterase i ... | 2004 | 15184114 |
| comparison of the proteome of methylobacterium extorquens am1 grown under methylotrophic and nonmethylotrophic conditions. | methylobacterium extorquens am1 is a facultative methylotrophic bacterium that is capable of growing in the presence of methanol as the sole carbon and energy source, but is also able to grow on a limited number of c(2), c(3), and c(4) compounds, for example succinate. this study provides a proteomic view of the cellular adaptation of m. extorquens am1 to growth on methanol and succinate, respectively. cytosolic proteins were separated by two-dimensional gel electrophoresis employing overlapping ... | 2004 | 15188393 |
| interaction between endophytic bacteria from citrus plants and the phytopathogenic bacteria xylella fastidiosa, causal agent of citrus-variegated chlorosis. | to isolate endophytic bacteria and xylella fastidiosa and also to evaluate whether the bacterial endophyte community contributes to citrus-variegated chlorosis (cvc) status in sweet orange (citrus sinensis [l.] osbeck cv. pera). | 2004 | 15189288 |
| [the aeration-dependent effect of vitamin b12 on dna biosynthesis in methylobacterium dichloromethanicum]. | the effect of vitamin b12 (cobalamin) on dna biosynthesis in methylobacterium dichloromethanicum was studied. when cultivated in media with methanol or dichloromethane, the bacterium produced approximately 10 micrograms corrinoids per g dry biomass, compared to about 7 micrograms/g when cultivated on ethanol or succinate. exogenous adenosylcobalamin (adocbl) stimulated dna biosynthesis in m. dichloromethanicum cells grown under poor aeration, the effect being mediated by adocb1-linked ribonucleo ... | 2004 | 15198026 |
| the principle of flux minimization and its application to estimate stationary fluxes in metabolic networks. | cellular functions are ultimately linked to metabolic fluxes brought about by thousands of chemical reactions and transport processes. the synthesis of the underlying enzymes and membrane transporters causes the cell a certain 'effort' of energy and external resources. considering that those cells should have had a selection advantage during natural evolution that enabled them to fulfil vital functions (such as growth, defence against toxic compounds, repair of dna alterations, etc.) with minima ... | 2004 | 15233787 |
| chloromethane-dependent expression of the cmu gene cluster of hyphomicrobium chloromethanicum. | the methylotrophic bacterium hyphomicrobium chloromethanicum cm2 can utilize chloromethane (ch(3)cl) as the sole carbon and energy source. previously genes cmub, cmuc, cmua, and fold were shown to be essential for the growth of methylobacterium chloromethanicum on ch(3)cl. these ch(3)cl-specific genes were subsequently detected in h. chloromethanicum. transposon and marker exchange mutagenesis studies were carried out to identify the genes essential for ch(3)cl metabolism in h. chloromethanicum. ... | 2004 | 15240299 |
| molecular analysis of shower curtain biofilm microbes. | households provide environments that encourage the formation of microbial communities, often as biofilms. such biofilms constitute potential reservoirs for pathogens, particularly for immune-compromised individuals. one household environment that potentially accumulates microbial biofilms is that provided by vinyl shower curtains. over time, vinyl shower curtains accumulate films, commonly referred to as "soap scum," which microscopy reveals are constituted of lush microbial biofilms. to determi ... | 2004 | 15240300 |
| molecular detection and isolation of facultatively methylotrophic bacteria, including methylobacterium podarium sp. nov., from the human foot microflora. | this is the first study to demonstrate that diverse methylotrophic bacteria occur in the human foot microflora. polymerase chain reaction (pcr) amplification of dna from the soles and toe clefts of feet of five subjects indicated methylobacterium strains to be present in all cases. polymerase chain reaction amplification also showed the gene for the alpha-subunit of methanol dehydrogenase (mxaf) to be present in all samples. two types of mxaf were recovered, one closest to that of methylobacteri ... | 2004 | 15250884 |
| mtbe biodegradation in a gravity flow, high-biomass retaining bioreactor. | the aerobic biodegradation of methyl tert-butyl ether (mtbe), a widely used fuel oxygenate, was investigated using a pilot-scale biomass-retaining bioreactor called a biomass concentrator reactor (bcr). the reactor was operated for a year at a flow rate of 2500 l/d on cincinnati dechlorinated tap water and an influent mtbe concentration of 5 mg/l. treatment efficiency of mtbe in the reactor during stable operations exceeded 99.9%. the upper 95% confidence levels of effluent mtbe concentrations a ... | 2004 | 15260347 |
| underdiagnosis of urinary tract infection caused by methylobacterium species with current standard processing of urine culture and its clinical implications. | methylobacterium species are environmental opportunistic bacteria, and urinary tract infection (uti) caused by these pathogens has not yet been documented. four cases of uti with methylobacterium bacteraemia in immunocompetent female patients are reported. their urine cultures, processed according to standard procedures (i.e. incubation at 35 degrees c in ambient air for 24 h before incubation at room temperature for a further 24 h), were either negative or positive for escherichia coli. special ... | 2004 | 15272062 |
| methylobacterium populi sp. nov., a novel aerobic, pink-pigmented, facultatively methylotrophic, methane-utilizing bacterium isolated from poplar trees (populus deltoides x nigra dn34). | a pink-pigmented, aerobic, facultatively methylotrophic bacterium, strain bj001t, was isolated from internal poplar tissues (populus deltoidesxnigra dn34) and identified as a member of the genus methylobacterium. phylogenetic analyses showed that strain bj001t is related to methylobacterium thiocyanatum, methylobacterium extorquens, methylobacterium zatmanii and methylobacterium rhodesianum. however, strain bj001t differed from these species in its carbon-source utilization pattern, particularly ... | 2004 | 15280290 |
| refined crystal structure of pseudoazurin from methylobacterium extorquens am1 at 1.5 a resolution. | the crystal structure of pseudoazurin from methylobacterium extorquens am1 (pazam1) has been solved by the molecular replacement method using copper-copper distances as translation parameters, which were obtained from difference patterson maps calculated with the synchrotron radiation data containing the multiwavelength anomalous-dispersion effect. the structure refinement was carried out by the use of molecular dynamics optimization and the restrained least-squares method. the final crystallogr ... | 1994 | 15299445 |
| bacterial communities and enzyme activities of pahs polluted soils. | three soils (i.e. a belgian soil, b-bt, a german soil, g, and an italian agricultural soil, i-bt) with different properties and hydrocarbon-pollution history with regard to their potential to degrade phenanthrene were investigated. a chemical and microbiological evaluation of soils was done using measurements of routine chemical properties, bacterial counts and several enzyme activities. the three soils showed different levels of polycyclic aromatic hydrocarbons (pahs), being their contamination ... | 2004 | 15331267 |
| genomic insights into methanotrophy: the complete genome sequence of methylococcus capsulatus (bath). | methanotrophs are ubiquitous bacteria that can use the greenhouse gas methane as a sole carbon and energy source for growth, thus playing major roles in global carbon cycles, and in particular, substantially reducing emissions of biologically generated methane to the atmosphere. despite their importance, and in contrast to organisms that play roles in other major parts of the carbon cycle such as photosynthesis, no genome-level studies have been published on the biology of methanotrophs. we repo ... | 2004 | 15383840 |
| computational design of reduced metabolic networks. | cellular functions are based on thousands of chemical reactions and transport processes, most of them being catalysed and regulated by specific proteins. systematic gene knockouts have provided evidence that this complex reaction network possesses considerable redundancy, that is, alternative routes exist along which signals and metabolic fluxes may be directed to accomplish an identical output behaviour. this property is of particular importance in cases where parts of the reaction network are ... | 2004 | 15457535 |
| production of surface active compounds from methane. | methane utilizing bacterial strain was isolated and found to produce a surface active compound while growing on methane. the maximum production of the surface active compound depended on pressure of methane and air in the reactor. the crude surface active compound was extracted from the cell free broth and its surface active and emulsification properties were studied. the purified surface active compound had a critical micelle concentration of 150 mg/l at ph 7.0. on the basis of surface tension ... | 2003 | 15527016 |
| methane utilization by methylobacterium species: new evidence but still no proof for an old controversy. | | 2004 | 15545410 |
| methylobacterium nodulans sp. nov., for a group of aerobic, facultatively methylotrophic, legume root-nodule-forming and nitrogen-fixing bacteria. | data on 72 non-pigmented bacterial strains that specifically induce nitrogen-fixing root nodules on the legume species crotalaria glaucoides, crotalaria perrottetii and crotalaria podocarpa are reviewed. by sds-page analysis of total protein patterns and by 16s rrna pcr-rflp, these strains form a homogeneous group that is separate from other legume root-nodule-forming bacteria. the 16s rrna gene-based phylogeny indicates that these bacteria belong to the genus methylobacterium. they can grow on ... | 2004 | 15545469 |
| bacterial population dynamics and community structure in a pharmaceutical manufacturing water supply system determined by real-time pcr and pcr-denaturing gradient gel electrophoresis. | to control bacteria in the pharmaceutical water supply system. | 2004 | 15546402 |
| [phylogenetic analysis of facultative methylotrophic bacteria of the genus methylobacterium]. | phylogenetic analysis of methylobacterium genus species using analysis of nucleotide sequence of 16s rrna genes has shown that methylobacterium zatmanii and m. rhodesianum possess high-level similarity with m. extorquens (99.4-98.8%), and m. fujisawaense and m. radiotolerans--with m. mesophilicum (98.9-97.8%). these species are also similar as to their phenotypical properties and their total dnas have also close similarity. thus, the right of m. zatmanii, m. rhodesianum, m. fujisawaense and m. r ... | 2004 | 15554292 |
| [biotransformation of organic substances by an immobilized associative bacterial culture]. | bacterial strains were screened to transform to end-products (carbon monoxide and water) elevated concentrations of acetone, acidic acid, and ethanol in a biocatalyst with an immobilized bacterial association cultivated on solid foam polyvinyl acetate (fpva). the innocuous association amalgamated paracoccus denitrificans vkm v-1324, pseudomonas esterophilus vkm v-1736d and achromobacter parvulus vkm v-1541d. the biocatalyst was tested with the help of classic methods and equipment for microbes c ... | 2004 | 15605736 |
| the atomic resolution structure of methanol dehydrogenase from methylobacterium extorquens. | the crystal structure of methanol dehydrogenase (mdh) from methylobacterium extorquens has been refined without stereochemical restraints at a resolution of 1.2 a. the high-resolution data have defined the conformation of the tricyclic pyrroloquinoline quinone (pqq) cofactor ring as entirely planar. the detailed definition of the active-site geometry has shown many features that are similar to the quinohaemo-protein alcohol dehydrogenases from comamonas testosteroni and pseudomonas putida, both ... | 2004 | 15608378 |
| characterization of micro-organisms isolated from dairy industry after cleaning and fogging disinfection with alkyl amine and peracetic acid. | to characterize micro-organisms isolated from norwegian dairy production plants after cleaning and fogging disinfection with alkyl amine/peracetic acid and to indicate reasons for survival. | 2005 | 15610421 |
| effects of ph amendment on metal working fluid wastewater biological treatment using a defined bacterial consortium. | the aim of this study was to determine whether ph amendment of a highly alkaline metal working fluid (mwf) wastewater would improve biological treatment in a bioreactor system following introduction of a bacterial inoculum (comprised of the following strains: agrobacterium radiobacter, comamonas testosteroni, methylobacterium mesophilicum, microbacterium esteraromaticum, and microbacterium saperdae). the ph values tested were 6, 7, 8, and 9. three replicate batch mode bioreactors inoculated with ... | 2005 | 15625673 |
| seasonal variations in location and population structure of endophytes in buds of scots pine. | we studied the location and distribution of a bacterial isolate, a mycobacterium sp., in buds of scots pine (pinus sylvestris l.). using a probe specific for the 16s rrna of the mycobacterium sp., the bacterium was found by in situ hybridization in the meristematic tissues of 40% of all bud samples examined. because we had previously found other bacterial and fungal endophytes in the meristematic tissues of scots pine buds, we studied their occurrence in buds during shoot development and dormanc ... | 2005 | 15631977 |
| how an enzyme binds the c1 carrier tetrahydromethanopterin. structure of the tetrahydromethanopterin-dependent formaldehyde-activating enzyme (fae) from methylobacterium extorquens am1. | tetrahydromethanopterin (h4 mpt) is a tetrahydrofolate analogue involved as a c1 carrier in the metabolism of various groups of microorganisms. how h4mpt is bound to the respective c1 unit converting enzymes remained elusive. we describe here the structure of the homopentameric formaldehyde-activating enzyme (fae) from methylobacterium extorquens am1 established at 2.0 angstrom without and at 1.9 angstrom with methylene-h4mpt bound. methylene-h4mpt is bound in an "s"-shaped conformation into the ... | 2005 | 15632161 |
| methylobacterium hispanicum sp. nov. and methylobacterium aquaticum sp. nov., isolated from drinking water. | members of the genus methylobacterium are ubiquitous in nature and can be isolated from almost any freshwater environment where dissolved oxygen exists. this genus is composed of a variety of pink-pigmented, facultatively methylotrophic (ppfm) bacteria. during a screening programme to monitor the bacterial population present in the drinking water of a municipal water supply in seville (spain) during the year 2003, five strains of ppfm bacteria were isolated and characterized. analysis of their c ... | 2005 | 15653888 |
| l-malyl-coenzyme a/beta-methylmalyl-coenzyme a lyase is involved in acetate assimilation of the isocitrate lyase-negative bacterium rhodobacter capsulatus. | cell extracts of rhodobacter capsulatus grown on acetate contained an apparent malate synthase activity but lacked isocitrate lyase activity. therefore, r. capsulatus cannot use the glyoxylate cycle for acetate assimilation, and a different pathway must exist. it is shown that the apparent malate synthase activity is due to the combination of a malyl-coenzyme a (coa) lyase and a malyl-coa-hydrolyzing enzyme. malyl-coa lyase activity was 20-fold up-regulated in acetate-grown cells versus glucose- ... | 2005 | 15687206 |
| identification of genes involved in the glyoxylate regeneration cycle in methylobacterium extorquens am1, including two new genes, meac and mead. | the glyoxylate regeneration cycle (grc) operates in serine cycle methylotrophs to effect the net conversion of acetyl coenzyme a to glyoxylate. mutants have been generated in several genes involved in the grc, and phenotypic analysis has been carried out to clarify their role in this cycle. | 2005 | 15687219 |
| [influence of the incubation temperature on the reaction of oligotrophic bacteria to stress]. | representatives of five genera of psychroactive oligotrophic bacteria, arcocella, renobacter, spirosoma, caulobacter, and methylobacterium, were for the first time shown to be capable of growing at a negative temperature (-2 degrees c). long-term cultivation (for 116 days) at a low temperature under limitation by the carbon source is stressful for oligotrophic bacteria and leads to the death of a part of the cell population. the number of viable cells of caulobacter crescentus decreased by two-t ... | 2004 | 15688934 |
| [methanotrophs and methylobacteria are found in woody plant tissues within a winter period]. | samples of tree seeds, buds, and needles collected within a winter period at ambient temperatures from -11 to -17 degrees c were analyzed for the presence of methylotrophic microflora. thin sections of blue spruce needles were found to contain bacteria morphologically close to pink-pigmented methylobacteria. the methylobacteria that were isolated in pure cultures from samples of linden seeds and buds, pine and blue spruce needles, as well as of lilac, maple, and apple buds, were classified into ... | 2004 | 15688941 |
| [identification of methylobacterium strains using sequence analysis of 16s rrna genes]. | | 2004 | 15688945 |
| archaea-like genes for c1-transfer enzymes in planctomycetes: phylogenetic implications of their unexpected presence in this phylum. | the unexpected presence of archaea-like genes for tetrahydromethanopterin (h4mpt)-dependent enzymes in the completely sequence geiome of the aerobic marine planctomycete pirellula sp. strain 1 ("rhodopirellula baltica") and in the currently sequenced genome of the aerobic freshwater planctomycete gemmata obscuriglobus strain uqm2246 revives the discussion on the origin of these genes in the bacterial domain. we compared the genomic arrangement of these genes in planctomyetes and methylotrophic p ... | 2004 | 15693614 |
| utility of environmental primers targeting ancient enzymes: methylotroph detection in lake washington. | methods have been explored for detection of methylotrophs in natural samples, using environmental primers based on genes involved in the tetrahydromethanopterin (h4mpt)-linked c1 transfer pathway. the underlying hypotheses were that the h4mpt-linked pathway is an ancient methylotrophy pathway, based on gene divergence, and that primers targeting more divergent genes will detect a broader variety of methylotrophs compared to the variety uncovered using probes and primers targeting highly conserve ... | 2004 | 15696380 |
| novel dephosphotetrahydromethanopterin biosynthesis genes discovered via mutagenesis in methylobacterium extorquens am1. | methylobacterium extorquens am1 was used to explore the genetics of dephosphotetrahydromethanopterin (dh(4)mpt) biosynthesis. strains with mutations in eight "archaeal-type" genes linked on the chromosome of m. extorquens am1 were analyzed for the ability to synthesize dh(4)mpt, and six were found to be dh(4)mpt negative. putative functions of these genes in dh(4)mpt biosynthesis are discussed. | 2005 | 15774894 |
| sugar composition and ft-ir analysis of exopolysaccharides produced by microbial isolates from paper mill slime deposits. | thirty exopolysaccharides (eps) produced by bacteria isolated from biofilms or slimelayers from different paper and board mills in finland, france and spain were subjected to size exclusion chromatography and sugar compositional analysis. high performance size exclusion chromatography (hpsec) analysis revealed that some samples were composed of several molecular weight populations. these samples were fractionated by size exclusion chromatography and pooled accordingly. principal components analy ... | 2005 | 15889397 |
| evidence for the presence of a cmua methyltransferase pathway in novel marine methyl halide-oxidizing bacteria. | marine bacteria that oxidized methyl bromide and methyl chloride were enriched and isolated from seawater samples. six methyl halide-oxidizing enrichments were established from which 13 isolates that grew on methyl bromide and methyl chloride as sole sources of carbon and energy were isolated and maintained. all isolates belonged to three different clades in the roseobacter group of the alpha subdivision of the proteobacteria and were distinct from leisingera methylohalidivorans, the only other ... | 2005 | 15892703 |
| [physicochemical and biological factors affecting atmospheric methane oxidation in gray forest soils]. | the decline of methane oxidizing activities in gray forest soil upon its conversion into arable land was shown to be caused by major changes in biotic and physicochemical properties of soil. using the method of immune serums, methane-oxidizing bacteria were detected in both forest and agricultural soils, but their populations differed significantly in both abundance and composition. in the forest soil, the number of methanotrophs was an order of magnitude higher than in arable soil, amounting to ... | 2005 | 15938403 |
| methylobacterium sp. bacteraemia due to a contaminated endoscope. | | 2005 | 15939507 |