| crystal structures of nadh:fmn oxidoreductase (emob) at different stages of catalysis. | edta has become a major organic pollutant in the environment because of its extreme usage and resistance to biodegradation. recently, two critical enzymes, edta monooxygenase (emoa) and nadh:fmn oxidoreductase (emob), belonging to the newly established two-component flavin-diffusible monooxygenase family, were identified in the edta degradation pathway in mesorhizobium sp. bnc1. emoa is an fmnh2-dependent enzyme that requires emob to provide fmnh2 for the conversion of edta to ethylenediaminedia ... | 2008 | 18701448 |
| isolation and characterization of alicycliphilus denitrificans strain bc, which grows on benzene with chlorate as the electron acceptor. | a bacterium, strain bc, was isolated from a benzene-degrading chlorate-reducing enrichment culture. strain bc degrades benzene in conjunction with chlorate reduction. cells of strain bc are short rods that are 0.6 microm wide and 1 to 2 microm long, are motile, and stain gram negative. strain bc grows on benzene and some other aromatic compounds with oxygen or in the absence of oxygen with chlorate as the electron acceptor. strain bc is a denitrifying bacterium, but it is not able to grow on ben ... | 2008 | 18791031 |
| influence of soil components on the biodegradation of benzene, toluene, ethylbenzene, and o-, m-, and p-xylenes by the newly isolated bacterium pseudoxanthomonas spadix bd-a59. | a bacterium designated strain bd-a59, able to degrade all six benzene, toluene, ethylbenzene, and o-, m-, and p-xylene (btex) compounds, was isolated by plating gasoline-contaminated sediment from a gasoline station in geoje, republic of korea, without enrichment, on minimal salts basal (msb) agar containing 0.01% yeast extract, with btex as the sole carbon and energy source. taxonomic analyses showed that the isolate belonged to pseudoxanthomonas spadix, and until now, the genus pseudoxanthomon ... | 2008 | 18835999 |
| adsorption characteristics of p(3hb) depolymerase as evaluated by surface plasmon resonance and atomic force microscopy. | molecular recognition of poly[(r)-3-hydroxybutyrate] (p(3hb)) depolymerase from ralstonia pickettii t1 to the surfaces of biodegradable aliphatic polyesters such as p(3hb) and poly(l-lactic acid) (plla) was examined from the viewpoints of kinetics and dynamics. to determine the kinetic parameters on the interaction between the substrate-binding domain (sbd) of p(3hb) depolymerase and various polymer substrates with different chemical structures, surface plasmon resonance (spr) measurements were ... | 2008 | 18939805 |
| improving the thermostability of n-carbamyl-d-amino acid amidohydrolase by error-prone pcr. | to facilitate the easier production of d-amino acids using n-carbamyl-d-amino acid amidohydrolase (dcase) in an immobilized form, we improved the enzymatic thermostability of highly soluble dcase-m3 of ralstonia pickettii using directed mutagenesis. six novel mutation sites were identified in this study, apart from several thermostability-related amino acid sites reported previously. the most thermostable mutant, in which the 12th amino acid had been changed from glutamine to leucine, showed a 7 ... | 2009 | 18985337 |
| the coastal environment and human health: microbial indicators, pathogens, sentinels and reservoirs. | innovative research relating oceans and human health is advancing our understanding of disease-causing organisms in coastal ecosystems. novel techniques are elucidating the loading, transport and fate of pathogens in coastal ecosystems, and identifying sources of contamination. this research is facilitating improved risk assessments for seafood consumers and those who use the oceans for recreation. a number of challenges still remain and define future directions of research and public policy. sa ... | 2008 | 19025674 |
| benzene degradation by ralstonia pickettii pko1 in the presence of the alternative substrate succinate. | the regulation of benzene degradation by ralstonia pickettii pko1 in the presence of the alternative substrate succinate was investigated in batch and continuous culture. in batch culture, r. pickettii pko1 achieved a maximum specific growth rate with benzene of 0.18 h(-1), while succinate allowed much faster growth (mu(max) = 0.5 h(-1)). under carbon excess conditions succinate repressed benzene consumption resulting in diauxic growth whereas under carbon-limited conditions in the chemostat bot ... | 2009 | 19039669 |
| evaluation of an immunochromatographic assay kit for rapid identification of mycobacterium tuberculosis complex in clinical isolates. | we evaluated a new immunochromatographic assay (ica) using mouse monoclonal anti-mpt64 antibody for rapid discrimination between mycobacterium tuberculosis and nontuberculous mycobacteria in clinical isolates. a study with mycobacteria and other organisms showed excellent sensitivity (approximately equal 99%) and specificity (100%) and an appropriate detection limit (10(5) cfu/ml) when tested with m. tuberculosis h37rv. this ica can simplify the identification of m. tuberculosis in clinical labo ... | 2009 | 19052177 |
| evaluation of an immunochromatographic assay kit for rapid identification of mycobacterium tuberculosis complex in clinical isolates. | we evaluated a new immunochromatographic assay (ica) using mouse monoclonal anti-mpt64 antibody for rapid discrimination between mycobacterium tuberculosis and nontuberculous mycobacteria in clinical isolates. a study with mycobacteria and other organisms showed excellent sensitivity (approximately equal 99%) and specificity (100%) and an appropriate detection limit (10(5) cfu/ml) when tested with m. tuberculosis h37rv. this ica can simplify the identification of m. tuberculosis in clinical labo ... | 2009 | 19052177 |
| insights on escherichia coli biofilm formation and inhibition from whole-transcriptome profiling. | biofilms transform independent cells into specialized cell communities. here are presented some insights into biofilm formation ascertained with the best-characterized strain, escherichia coli. investigations of biofilm formation and inhibition with this strain using whole-transcriptome profiling coupled to phenotypic assays, in vivo dna binding studies and isogenic mutants have led to discoveries related to the role of stress, to the role of intra- and interspecies cell signalling, to the impac ... | 2009 | 19125816 |
| isolation and identification of cellulose-binding proteins from sheep rumen contents. | to extend our understanding of the mechanisms of plant cell wall degradation in the rumen, cellulose-binding proteins (cbps) from the contents of a sheep rumen were directly isolated and identified using a metaproteomics approach. the rumen cbps were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, and some cbps revealed endoglucanase activities toward carboxymethyl cellulose. using mass spectrometry analyses, four cbps were identified and annotated as known proteins from ... | 2009 | 19151184 |
| ralstonia pickettii septicemia in a dog with immune-mediated thrombocytopenia. | | 2009 | 19175738 |
| transmembrane passage of hydrophobic compounds through a protein channel wall. | membrane proteins that transport hydrophobic compounds have important roles in multi-drug resistance and can cause a number of diseases, underscoring the importance of protein-mediated transport of hydrophobic compounds. hydrophobic compounds readily partition into regular membrane lipid bilayers, and their transport through an aqueous protein channel is energetically unfavourable. alternative transport models involving acquisition from the lipid bilayer by lateral diffusion have been proposed f ... | 2009 | 19182779 |
| characterization of a novel 3-hydroxybutyrate dehydrogenase from ralstonia pickettii t1. | we previously reported that the activities of two 3-hydroxybutyrate dehydrogenases (bdh1 and bdh2) were greatly influenced by culture conditions when ralstonia pickettii t1, a strain growing on extracellular poly-3-hydroxybutyrate (phb), was grown on different carbon sources such as 3hb and succinate. in this study, knockout mutants of bdh1 or bdh2 were constructed and characterized under different culture conditions. in addition, a novel bdh (bdh3) was found in bdh2 mutants, and bdh3 was cloned ... | 2009 | 19219638 |
| development of non-antibiotic-resistant, chromosomally based, constitutive and inducible expression systems for aroa-attenuated salmonella enterica serovar typhimurium. | live-vaccine delivery systems expressing two model antigens from mycoplasma hyopneumoniae, f2(p97) (adh) and nrdf, were constructed using salmonella enterica serovar typhimurium aroa (stm-1), and immunogenicity in mice was evaluated. recombinant plasmid-based expression (pbe) and chromosomally based expression (cbe) systems were constructed. the pbe system was formed by cloning both antigen genes into pjla507 to create an operon downstream of temperature-inducible promoters. constitutive cbe was ... | 2009 | 19223478 |
| principles of bone marrow transplantation (bmt): providing optimal veterinary and husbandry care to irradiated mice in bmt studies. | bone marrow transplantation (bmt) is the treatment of choice for many leukemias, solid tumors, and metabolic diseases. the field of bone marrow research is highly dependent on in vivo experimentation, because in vitro techniques do not mimic these complicated in vivo systems. therefore, understanding the medical and husbandry care needs of these transiently immunodeficient bone marrow recipient animals is crucial for researchers, veterinary and animal care personnel. here we discuss the principl ... | 2009 | 19245745 |
| low rates of pseudomonas aeruginosa misidentification in isolates from cystic fibrosis patients. | pseudomonas aeruginosa is an important cause of pulmonary infection in cystic fibrosis (cf). its correct identification ensures effective patient management and infection control strategies. however, little is known about how often cf sputum isolates are falsely identified as p. aeruginosa. we used p. aeruginosa-specific duplex real-time pcr assays to determine if 2,267 p. aeruginosa sputum isolates from 561 cf patients were correctly identified by 17 australian clinical microbiology laboratorie ... | 2009 | 19261796 |
| siderophore production by marine-derived fungi. | siderophore production by marine-derived fungi has not been extensively explored. three studies have investigated the ability of marine-derived fungi to produce siderophores in response to iron limitation [(vala et al. in indian j mar sci 29:339-340, 2000; can j microbiol 52:603-607, 2006); baakza et al. in j exp mar biol ecol 311:1-9, 2004]. in all, 24 of 28 marine fungal strains were found to secrete hydroxamate or carboxylate siderophores; no evidence was found for production of catecholate s ... | 2009 | 19350395 |
| genetic and biochemical analyses of chlorobenzene degradation gene clusters in pandoraea sp. strain mcb032. | pandoraea sp. strain mcb032 was isolated as an emerging chlorobenzene degrader from a functionally stable bioreactor where species succession had occurred. in this study, two gene clusters encoding chlorobenzene metabolic functions have been cloned. within the cbs gene cluster, cbsa and cbsb are similar to the chlorobenzene dioxygenase and the cis-chlorobenzene dihydrodiol dehydrogenase in ralstonia sp. js705 and shown to transform chlorobenzene to 3-chlorocatechol. the clc gene cluster shows st ... | 2009 | 19365620 |
| outbreak of ralstonia pickettii bacteraemia in patients with haematological malignancies and haematopoietic stem cell transplant recipients. | | 2009 | 19376610 |
| ralstonia pickettii-induced ataxia in immunodeficient mice. | we report here the characterization of an asymmetric ataxia syndrome (head tilt and circling, with death in the most severe cases) demonstrated by profoundly immunodeficient mice housed at the institut curie spf facility. the immune system of the affected mice had been genetically modified so that they were deficient in both b and t cells. extensive bacteriologic, parasitic, serologic, and histopathologic analysis of the affected animals and their healthy controls led us to identify ralstonia pi ... | 2009 | 19389312 |
| microbial co-habitation and lateral gene transfer: what transposases can tell us. | determining the habitat range for various microbes is not a simple, straightforward matter, as habitats interlace, microbes move between habitats, and microbial communities change over time. in this study, we explore an approach using the history of lateral gene transfer recorded in microbial genomes to begin to answer two key questions: where have you been and who have you been with? | 2009 | 19393086 |
| [comparison between agar dilution and three other methods for determining the susceptibility of 228 clinical isolates of non-fermenting gram-negative rods]. | susceptibility testing for non-fermenting gram-negative rods (nfgnr) is problematic; valid methods are needed for this purpose. | 2009 | 19406523 |
| involvement of two transport systems and a specific porin in the uptake of phthalate by burkholderia spp. | burkholderia spp. that degrade phthalate have an abc transporter-type phthalate transport system (ophfgh) and a specific porin (ophp) in addition to a permease-type phthalate transporter (ophd). ophfgh has a lower k(m) and higher v(max) than ophd, which affects how the bacteria grow. ophp is involved in both mechanisms of transport. | 2009 | 19429613 |
| ralstonia pickettii meningitis in a child with hydrocephalus. | | 2009 | 19449287 |
| interactions between hyphosphere-associated bacteria and the fungus cladosporium herbarum on aquatic leaf litter. | we investigated microbial interactions of aquatic bacteria associated with hyphae (the hyphosphere) of freshwater fungi on leaf litter. bacteria were isolated directly from the hyphae of fungi from sedimented leaves of a small stream in the national park "lower oder," germany. to investigate interactions, bacteria and fungi were pairwise co-cultivated on leaf-extract medium and in microcosms loaded with leaves. the performance of fungi and bacteria was monitored by measuring growth, enzyme produ ... | 2009 | 19452199 |
| isolation and molecular characterization of a novel d-hydantoinase from jannaschia sp. ccs1. | hydantoinases (hyds) are important enzymes for industrial production of optically pure amino acids, which are widely used as precursors for various semi-synthetic antibiotics. by a process coupling genomic data mining with activity screening, a new hydantoinase, tentatively designated hyd(js), was identified from jannaschia sp. ccs1 and overexpressed in escherichia coli. the specific activity of hyd(js) on d,l-p-hydroxyphenylhydantoin as the substrate was three times higher than that of the hyda ... | 2009 | 19490017 |
| metabolic capacity of sinorhizobium (ensifer) meliloti strains as determined by phenotype microarray analysis. | sinorhizobium meliloti is a soil bacterium that fixes atmospheric nitrogen in plant roots. the high genetic diversity of its natural populations has been the subject of extensive analysis. recent genomic studies of several isolates revealed a high content of variable genes, suggesting a correspondingly large phenotypic differentiation among strains of s. meliloti. here, using the phenotype microarray (pm) system, hundreds of different growth conditions were tested in order to compare the metabol ... | 2009 | 19561177 |
| glycosylation of fluorophenols by plant cell cultures. | fluoroaromatic compounds are used as agrochemicals and released into environment as pollutants. glycosylation of 2-, 3-, and 4-fluorophenols using plant cell cultures of nicotiana tabacum was investigated to elucidate their potential to metabolize these compounds. cultured n. tabacum cells converted 2-fluorophenol into its beta-glucoside (60%) and beta-gentiobioside (10%). 4-fluorophenol was also glycosylated to its beta-glucoside (32%) and beta-gentiobioside (6%) by n. tabacum cells. on the oth ... | 2009 | 19564930 |
| metagenomics reveals diversity and abundance of meta-cleavage pathways in microbial communities from soil highly contaminated with jet fuel under air-sparging bioremediation. | the extradiol dioxygenase diversity of a site highly contaminated with aliphatic and aromatic hydrocarbons under air-sparging treatment was assessed by functional screening of a fosmid library in escherichia coli with catechol as substrate. the 235 positive clones from inserts of dna extracted from contaminated soil were equivalent to one extradiol dioxygenase-encoding gene per 3.6 mb of dna screened, indicating a strong selection for genes encoding this function. three subfamilies were identifi ... | 2009 | 19575758 |
| airway infection with a novel cupriavidus species in persons with cystic fibrosis. | we describe the recovery and identification of a bacterium that represents a new species of the genus cupriavidus from cultures of respiratory tract specimens from two patients with cystic fibrosis (cf). the elucidation of the role of this species in cf lung disease will require an evaluation of a greater number of cases. | 2009 | 19605576 |
| [acute bacterial prostatitis by ralstonia pickettii: clinical and epidemiological considerations of excepcional observation]. | | 2009 | 19615483 |
| investigation of shell disease in map turtles (graptemys spp.). | nineteen map turtles (graptemys spp.) maintained under natural conditions were investigated because of chronic shell abnormalities. animals were evaluated using a novel shell scoring system that divided the 54 scutes into six regions, with each region scored for lesion extent and severity, and summated to produce a total shell disease score (tsds). complete blood counts and various biochemistry analytes (total protein, albumin, globulin, urea, uric acid, 25-hydroxycholecalciferol, phosphorus, an ... | 2009 | 19617474 |
| [a community acquired pneumonia case caused by ralstonia pickettii]. | ralstonia pickettii, formerly known as burkholderia pickettii, is a non-fermentative gram-negative bacillus. it is emerging as an opportunistic pathogen both in the hospital setting and in the environment, leading to outbreaks especially in the intensive care units. the available literature revealed two case reports of pneumonia associated with r. pickettii in adults. in this report, a case of pneumoniae due to r. pickettii, in a patient with chronic obstructive pulmonary disease was presented. ... | 2009 | 19621622 |
| rapid real-time pcr assays for detection of klebsiella pneumoniae with the rmpa or maga genes associated with the hypermucoviscosity phenotype: screening of nonhuman primates. | the relationship of mucoviscosity-associated (maga) and/or regulator of mucoid phenotype (rmpa) genes to the klebsiella pneumoniae hypermucoviscosity (hmv) phenotype has been reported. we previously demonstrated that rmpa+ k. pneumoniae can cause serious disease in african green monkeys and isolated rmpa+ and maga+ hmv k. pneumoniae from other species of non-human primates. to rapidly screen african green monkeys/non-human primates for these infections, we developed three real-time pcr assays. t ... | 2009 | 19644019 |
| diversity, abundance, and consistency of microbial oxygenase expression and biodegradation in a shallow contaminated aquifer. | the diversity of rieske dioxygenase genes and short-term temporal variability in the abundance of two selected dioxygenase gene sequences were examined in a naphthalene-rich, coal tar waste-contaminated subsurface study site. using a previously published pcr-based approach (s. m. ní chadhain, r. s. norman, k. v. pesce, j. j. kukor, and g. j. zylstra, appl. environ. microbiol. 72:4078-4087, 2006) a broad suite of genes was detected, ranging from dioxygenase sequences associated with rhodococcus a ... | 2009 | 19700556 |
| degradation of mixtures of aromatic and chloroaliphatic hydrocarbons by aromatic hydrocarbon-degrading bacteria. | the well-characterized toluene-oxidizing bacteria pseudomonas putida paw1, p. putida f1, p. mendocina kr1, burkholderia cepacia g4, b. cepacia js150, and ralstonia pickettii pko1, as well as several strains of bacteria isolated from activated sludge, were examined for their ability to degrade mixtures of aromatic and chloroaliphatic hydrocarbons. collectively, the strains tested were able to transform all of the 14 aromatic hydrocarbons included in the mixtures, with most strains degrading five ... | 2003 | 19719688 |
| diversity, epidemiology, and genetics of class d beta-lactamases. | class d beta-lactamase-mediated resistance to beta-lactams has been increasingly reported during the last decade. those enzymes also known as oxacillinases or oxas are widely distributed among gram negatives. genes encoding class d beta-lactamases are known to be intrinsic in many gram-negative rods, including acinetobacter baumannii and pseudomonas aeruginosa, but play a minor role in natural resistance phenotypes. the oxas (ca. 150 variants reported so far) are characterized by an important ge ... | 2010 | 19721065 |
| diversity, epidemiology, and genetics of class d beta-lactamases. | class d beta-lactamase-mediated resistance to beta-lactams has been increasingly reported during the last decade. those enzymes also known as oxacillinases or oxas are widely distributed among gram negatives. genes encoding class d beta-lactamases are known to be intrinsic in many gram-negative rods, including acinetobacter baumannii and pseudomonas aeruginosa, but play a minor role in natural resistance phenotypes. the oxas (ca. 150 variants reported so far) are characterized by an important ge ... | 2010 | 19721065 |
| characterization of regulatory element rp3 of regulation of beta-lactamases from ralstonia pickettii. | the two chromosomally encoded beta-lactamases, oxa-22 and oxa-60, from ralstonia pickettii are inducible by beta-lactam molecules. disruption of rp3 abolished induction of both beta-lactamases and the resistance to ph, osmolarity and survival in the stationary phase, suggesting that rp3 might be a global regulator. interactions between rp3, oxa-22 and oxa-60 were investigated at a transcript and protein level using 5'-rapid amplification of cdna ends experiments, real-time reverse transcription ... | 2009 | 19817868 |
| an immunocompetent patient presenting with severe septic arthritis due to ralstonia pickettii identified by molecular-based assays: a case report. | ralstonia pickettii is an infrequent pathogen of invasive infections in healthy individuals. the microorganism is supposed to be of relatively low virulence, but can cause infections, mainly of the respiratory tract, in immunocompromised and cystic fibrosis patients. ralstonia pickettii has also been associated with hospital outbreaks related to contamination of products used for medical care and laboratory diagnosis. | 2009 | 19830053 |
| analysis of two gene clusters involved in the degradation of 4-fluorophenol by arthrobacter sp. strain if1. | arthrobacter sp. strain if1 is able to grow on 4-fluorophenol (4-fp) as a sole source of carbon and energy. to clone the 4-fp degradation genes, dna libraries were constructed and screened with a probe obtained by pcr using primers designed on the basis of conserved regions of aromatic two-component monooxygenases. sequencing of positive clones yielded two gene clusters, each harboring a gene encoding a monooxygenase with high sequence similarity to the oxygenase component of 4-nitrophenol and 4 ... | 2009 | 19837837 |
| general trends in trace element utilization revealed by comparative genomic analyses of co, cu, mo, ni, and se. | trace elements are used by all organisms and provide proteins with unique coordination and catalytic and electron transfer properties. although many trace element-containing proteins are well characterized, little is known about the general trends in trace element utilization. we carried out comparative genomic analyses of copper, molybdenum, nickel, cobalt (in the form of vitamin b(12)), and selenium (in the form of selenocysteine) in 747 sequenced organisms at the following levels: (i) transpo ... | 2010 | 19887375 |
| general trends in trace element utilization revealed by comparative genomic analyses of co, cu, mo, ni, and se. | trace elements are used by all organisms and provide proteins with unique coordination and catalytic and electron transfer properties. although many trace element-containing proteins are well characterized, little is known about the general trends in trace element utilization. we carried out comparative genomic analyses of copper, molybdenum, nickel, cobalt (in the form of vitamin b(12)), and selenium (in the form of selenocysteine) in 747 sequenced organisms at the following levels: (i) transpo ... | 2010 | 19887375 |
| genomic and biological analysis of phage xfas53 and related prophages of xylella fastidiosa. | we report the plaque propagation and genomic analysis of xfas53, a temperate phage of xylella fastidiosa. xfas53 was isolated from supernatants of x. fastidiosa strain 53 and forms plaques on the sequenced strain temecula. xfas53 forms short-tailed virions, morphologically similar to podophage p22. the 36.7-kb genome is predicted to encode 45 proteins. the xfas53 terminase and structural genes are related at a protein and gene order level to p22. the left arm of the xfas53 genome has over 90% nu ... | 2010 | 19897657 |
| genomic and biological analysis of phage xfas53 and related prophages of xylella fastidiosa. | we report the plaque propagation and genomic analysis of xfas53, a temperate phage of xylella fastidiosa. xfas53 was isolated from supernatants of x. fastidiosa strain 53 and forms plaques on the sequenced strain temecula. xfas53 forms short-tailed virions, morphologically similar to podophage p22. the 36.7-kb genome is predicted to encode 45 proteins. the xfas53 terminase and structural genes are related at a protein and gene order level to p22. the left arm of the xfas53 genome has over 90% nu ... | 2010 | 19897657 |
| novel insights about class 2 integrons from experimental and genomic epidemiology. | in order to contribute to the knowledge of the architecture and epidemiology of class 2 integrons, we performed a class 2 integron molecular survey in which we analyzed 726 isolates in two bacterial populations from environmental and nonepidemiologically related clinical samples, respectively, collected from 1982 to 2007. we recovered the inti2 gene from 130 of 726 isolates, most of which were clinical isolates, and only 1 (a psychrophilic pseudomonas sp.) was from a water sample. unlike the wid ... | 2010 | 19917745 |
| novel insights about class 2 integrons from experimental and genomic epidemiology. | in order to contribute to the knowledge of the architecture and epidemiology of class 2 integrons, we performed a class 2 integron molecular survey in which we analyzed 726 isolates in two bacterial populations from environmental and nonepidemiologically related clinical samples, respectively, collected from 1982 to 2007. we recovered the inti2 gene from 130 of 726 isolates, most of which were clinical isolates, and only 1 (a psychrophilic pseudomonas sp.) was from a water sample. unlike the wid ... | 2010 | 19917745 |
| a beta-barrel outer membrane protein facilitates cellular uptake of polychlorophenols in cupriavidus necator. | the tcprxabcyd operon of cupriavidus necator jmp134 is involved in the degradation of 2,4,6-trichlorophenol (tcp). all of the gene products except tcpy have assigned functions in tcp metabolism. sequence comparison identified tcpy as a member of cog4313, a group of hypothetical proteins. tcpy has a signal peptide, indicating it is a membrane or secreted protein. secondary structure and topology analysis indicated tcpy as a beta-barrel outer membrane protein, similar to the escherichia coli outer ... | 2010 | 19937267 |
| a beta-barrel outer membrane protein facilitates cellular uptake of polychlorophenols in cupriavidus necator. | the tcprxabcyd operon of cupriavidus necator jmp134 is involved in the degradation of 2,4,6-trichlorophenol (tcp). all of the gene products except tcpy have assigned functions in tcp metabolism. sequence comparison identified tcpy as a member of cog4313, a group of hypothetical proteins. tcpy has a signal peptide, indicating it is a membrane or secreted protein. secondary structure and topology analysis indicated tcpy as a beta-barrel outer membrane protein, similar to the escherichia coli outer ... | 2010 | 19937267 |
| novel tn4371-ice like element in ralstonia pickettii and genome mining for comparative elements. | integrative conjugative elements (ices) are important factors in the plasticity of microbial genomes. an element related to the ice tn4371 was discovered during a bioinformatic search of the ralstonia pickettii 12j genome. this element was analysed and further searches carried out for additional elements.a pcr method was designed to detect and characterise new elements of this type based on this scaffold and a culture collection of fifty-eight ralstonia pickettii and ralstonia insidiosa strains ... | 2009 | 19941653 |
| use of hydrogenophaga pseudoflava penetration to quantitatively assess the impact of filtration parameters for 0.2-micrometer-pore-size filters. | filters rated as having a 0.2-microm pore size (0.2-microm-rated filters) are used in laboratory and manufacturing settings for diverse applications of bacterial and particle removal from process fluids, analytical test articles, and gasses. using hydrogenophaga pseudoflava, a diminutive bacterium with an unusual geometry (i.e., it is very thin), we evaluated passage through 0.2-microm-rated filters and the impact of filtration process parameters and bacterial challenge density. we show that con ... | 2010 | 19966023 |
| use of hydrogenophaga pseudoflava penetration to quantitatively assess the impact of filtration parameters for 0.2-micrometer-pore-size filters. | filters rated as having a 0.2-microm pore size (0.2-microm-rated filters) are used in laboratory and manufacturing settings for diverse applications of bacterial and particle removal from process fluids, analytical test articles, and gasses. using hydrogenophaga pseudoflava, a diminutive bacterium with an unusual geometry (i.e., it is very thin), we evaluated passage through 0.2-microm-rated filters and the impact of filtration process parameters and bacterial challenge density. we show that con ... | 2010 | 19966023 |
| degradation and adsorption characteristics of phb depolymerase as revealed by kinetics of mutant enzymes with amino acid substitution in substrate-binding domain. | extracelluar poly[(r)-3-hydroxybutyrate] (phb) depolymerase (phaz(rpit1)) from ralstonia pickettii t1 adsorbs to phb surface via its substrate-binding domain (sbd) to enhance phb degradation. our previous study combining pcr random mutagenesis with the determination of phb degradation levels of mutant enzymes suggested that ser, tyr, val, ala, and leu residues in sbd are probably involved in the enzymatic adsorption to and degradation of phb. in the present study, the effects of mutations at leu ... | 2010 | 20058938 |
| an automated system for rapid non-destructive enumeration of growing microbes. | the power and simplicity of visual colony counting have made it the mainstay of microbiological analysis for more than 130 years. a disadvantage of the method is the long time required to generate visible colonies from cells in a sample. new rapid testing technologies generally have failed to maintain one or more of the major advantages of culture-based methods. | 2010 | 20062794 |
| fatal infection caused by cupriavidus gilardii in a child with aplastic anemia. | cupriavidus gilardii is a gram-negative bacterium that has rarely been associated with human infections. we report a fatal case of sepsis caused by c. gilardii in a previously healthy 12-year-old female. | 2010 | 20071544 |
| characterization of a cryptic plasmid pd403 from lactobacillus plantarum and construction of shuttle vectors based on its replicon. | a cryptic plasmid pd403 was isolated from lactobacillus plantarum d403 derived from fermented dairy products. it was 2,791 bp in size with a g+c content of 37%. nucleotide sequence analysis revealed two open reading frames, orf1 and orf2. orf1 (318 amino acids) was identified as a replication protein (repa). orf2 (137 amino acids) shared 31% similarity with the transcriptional regulator of ralstonia pickettii 12d. functional investigation indicated that orf2 (tra) had the ability of improving th ... | 2010 | 20077035 |
| efficient biocatalytic production of d-4-hydroxyphenylglycine by whole cells of recombinant ralstonia pickettii. | the first establishment of a homologous expression system in the host ralstonia pickettii cgmcc1596 using the compatible broad-host-range plasmid pwb5 is described. when whole cells of the recombinant strain r. pickettii mmyy01 (cgmcc1596/pyy05) were used as the biocatalyst to transform dl-4-hydroxyphenylhydantoin (dl-hph) to d-4-hydroxyphenylglycine (d-hpg), the conversion rate reached 94 % in first 9 h, at a production rate of 2.8 g l(-1) h(-1), with the rapid reduction of the intermediate [n- ... | 2010 | 20140718 |
| effects of bovine milk lactoperoxidase system on some bacteria. | bovine lactoperoxidase (lpo) was purified from skimmed milk using amberlite cg-50-h+ resin, cm sephadex c-50 ion-exchange chromatography, and sephadex g-100 gel filtration chromatography. lactoperoxidase was purified 20.45-fold with a yield of 28.8%. purity of enzyme checked by sodium dodecyl sulphate-polyacrylamide gel electrophoresis method and a single band was observed. km was 0.25 mm at 20 degrees c, vmax value was 7.95 micromol/ml min at 20 degrees c (ph 6.0). antibacterial study was done ... | 2010 | 20198919 |
| rapid differentiation of francisella species and subspecies by fluorescent in situ hybridization targeting the 23s rrna. | francisella (f.) tularensis is the causative agent of tularemia. due to its low infectious dose, ease of dissemination and high case fatality rate, f. tularensis was the subject in diverse biological weapons programs and is among the top six agents with high potential if misused in bioterrorism. microbiological diagnosis is cumbersome and time-consuming. methods for the direct detection of the pathogen (immunofluorescence, pcr) have been developed but are restricted to reference laboratories. | 2010 | 20205957 |
| the complete multipartite genome sequence of cupriavidus necator jmp134, a versatile pollutant degrader. | cupriavidus necator jmp134 is a gram-negative beta-proteobacterium able to grow on a variety of aromatic and chloroaromatic compounds as its sole carbon and energy source. | 2010 | 20339589 |
| identification of diverse antimicrobial resistance determinants carried on bacterial, plasmid, or viral metagenomes from an activated sludge microbial assemblage. | using both sequence- and function-based metagenomic approaches, multiple antibiotic resistance determinants were identified within metagenomic libraries constructed from dna extracted from bacterial chromosomes, plasmids, or viruses within an activated sludge microbial assemblage. metagenomic clones and a plasmid that in escherichia coli expressed resistance to chloramphenicol, ampicillin, or kanamycin were isolated, with many cloned dna sequences lacking any significant homology to known antibi ... | 2010 | 20382816 |
| comonomer compositional distribution, physical properties, and enzymatic degradability of bacterial poly(3-hydroxybutyrate-co-3-hydroxy-4-methylvalerate) copolyesters. | poly(3-hydroxybutyrate-co-3-hydroxy-4-methylvalerate) (p(3hb-co-3h4mv)) was synthesized by using ralstonia eutropha strain phb(-)4 (pha-negative mutant) harboring with the n149s/d171g double mutation of pha synthase gene from aeromonas caviae (phac(ac) nsdg mutant) with 4-methylvaleric acid and fructose as the carbon sources. because the microbially synthesized p(3hb-co-3h4mv) samples were found to have broad comonomer compositional distribution, p(3hb-co-3h4mv) samples with 3h4mv content of 7, ... | 2010 | 20441146 |
| pseudo-outbreak of cupriavidus pauculus infection at an outpatient clinic related to rinsing culturette swabs in tap water. | cupriavidus pauculus is a water microorganism rarely isolated from clinical specimens. we describe a pseudo-outbreak in which multiple strains that were associated with moistening of culturette swabs with tap water were isolated from a single clinic before collecting the patient specimen. | 2010 | 20444965 |
| the complete genome sequence of cupriavidus metallidurans strain ch34, a master survivalist in harsh and anthropogenic environments. | many bacteria in the environment have adapted to the presence of toxic heavy metals. over the last 30 years, this heavy metal tolerance was the subject of extensive research. the bacterium cupriavidus metallidurans strain ch34, originally isolated by us in 1976 from a metal processing factory, is considered a major model organism in this field because it withstands milli-molar range concentrations of over 20 different heavy metal ions. this tolerance is mostly achieved by rapid ion efflux but al ... | 2010 | 20463976 |
| potential of aerobic denitrification by pseudomonas stutzeri tr2 to reduce nitrous oxide emissions from wastewater treatment plants. | in contrast to most denitrifiers studied so far, pseudomonas stutzeri tr2 produces low levels of nitrous oxide (n(2)o) even under aerobic conditions. we compared the denitrification activity of strain tr2 with those of various denitrifiers in an artificial medium that was derived from piggery wastewater. strain tr2 exhibited strong denitrification activity and produced little n(2)o under all conditions tested. its growth rate under denitrifying conditions was near comparable to that under aerobi ... | 2010 | 20495048 |
| incidence, relevance and response for ralsfonia respiratory infections. | cases of ralstonia colonization/infection occasionally reported by hospitals has generated increased interest in an organism previously little known to most clinicians. our goal was to determine the incidence of respiratory colonizations and infections involving ralstonia and the association of mechanical ventilation (limited to reports on respiratory-related occurrences in the u.s.a.) and propose a decision chart to assist response. | 2010 | 20499534 |
| nitroaromatic compounds, from synthesis to biodegradation. | nitroaromatic compounds are relatively rare in nature and have been introduced into the environment mainly by human activities. this important class of industrial chemicals is widely used in the synthesis of many diverse products, including dyes, polymers, pesticides, and explosives. unfortunately, their extensive use has led to environmental contamination of soil and groundwater. the nitro group, which provides chemical and functional diversity in these molecules, also contributes to the recalc ... | 2010 | 20508249 |
| the structure of phaz7 at atomic (1.2 a) resolution reveals details of the active site and suggests a substrate-binding mode. | poly-(r)-hydroxyalkanoates (phas) are bacterial polyesters that are degraded by a group of enzymes known as pha depolymerases. paucimonas lemoignei phaz7 depolymerase is the only extracellular depolymerase that has been described as being active towards amorphous phas. a previously determined crystal structure of phaz7 revealed an alpha/beta-hydrolase fold and a ser-his-asp catalytic triad. in order to address questions regarding the catalytic mechanism and substrate binding, the atomic resoluti ... | 2010 | 20516591 |
| bacterial gut symbionts contribute to seed digestion in an omnivorous beetle. | obligate bacterial symbionts alter the diets of host animals in numerous ways, but the ecological roles of facultative bacterial residents that colonize insect guts remain unclear. carabid beetles are a common group of beneficial insects appreciated for their ability to consume insect prey and seeds, but the contributions of microbes to diet diversification in this and similar groups of facultative granivores are largely unknown. | 2010 | 20520762 |
| efficacy of various chemical disinfectants on biofilms formed in spacecraft potable water system components. | as the provision of potable water is critical for successful habitation of the international space station (iss), life support systems were installed in december 2008 to recycle both humidity from the atmosphere and urine to conserve available water in the station. in-flight pre-consumption testing from the dispensing needle at the potable water dispenser (pwd) indicated that bacterial concentrations exceeded the current iss specifications of 50 colony-forming units (cfu) ml(-1). subsequent inve ... | 2010 | 20544435 |
| the o28 antigen gene clusters of salmonella enterica subsp. enterica serovar dakar and serovar pomona are different. | a 10 kb o-antigen gene cluster was sequenced from a salmonella enterica subsp. enterica dakar o28 reference strain and from two s. pomona serogroup o28 isolates. the two s. pomona o antigen gene clusters showed only moderate identity with the s. dakar o28 gene cluster, suggesting that the o antigen oligosaccharides may contain one or more sugars conferring the o28 epitope but may otherwise be different. these novel findings are absolutely critical for the correct interpretation of molecular sero ... | 2010 | 20652070 |
| biosequestration via cooperative binding of copper by ralstonia pickettii. | ralstonia pickettii isolated from copper-contaminated lake sediment are adapted to high levels of copper after 100 years of selective pressure. two r. pickettii strains (12d and 12j) were selected for the studies reported herein due to their distinct differences in genomic structure, different metal resistance patterns and carriage of a filamentous phage. copper sequestration studies revealed that these strains could bind up to 27.44 (12d) and 38.19 (12j) mg copper per g dry weight of cells and ... | 2010 | 20662391 |
| reversible post-translational carboxylation modulates the enzymatic activity of n-acetyl-l-ornithine transcarbamylase. | n-acetyl-l-ornithine transcarbamylase (aotcase), rather than ornithine transcarbamylase (otcase), is the essential carbamylase enzyme in the arginine biosynthesis of several plant and human pathogens. the specificity of this unique enzyme provides a potential target for controlling the spread of these pathogens. recently, several crystal structures of aotcase from xanthomonas campestris (xc) have been determined. in these structures, an unexplained electron density at the tip of the lys302 side ... | 2010 | 20695527 |
| sea-land transitions in isopods: pattern of symbiont distribution in two species of intertidal isopods ligia pallasii and ligia occidentalis in the eastern pacific. | studies of microbial associations of intertidal isopods in the primitive genus ligia (oniscidea, isopoda) can help our understanding of the formation of symbioses during sea-land transitions, as terrestrial oniscidean isopods have previously been found to house symbionts in their hepatopancreas. ligia pallasii and ligia occidentalis co-occur in the high intertidal zone along the eastern pacific with a large zone of range overlap and both species showing patchy distributions. in 16s rrna clone li ... | 2010 | 20730112 |
| oxa-134, a naturally occurring carbapenem-hydrolyzing class d beta-lactamase from acinetobacter lwoffii. | acinetobacter lwoffii, a species whose natural habitat is the human skin, intrinsically possesses a chromosomal gene encoding a carbapenem-hydrolyzing class d β-lactamase, oxa-134. this species may therefore constitute a reservoir for carbapenemase genes that may spread among other acinetobacter species. | 2010 | 20837764 |
| genomic analysis and relatedness of p2-like phages of the burkholderia cepacia complex. | the burkholderia cepacia complex (bcc) is comprised of at least seventeen gram-negative species that cause infections in cystic fibrosis patients. because bcc bacteria are broadly antibiotic resistant, phage therapy is currently being investigated as a possible alternative treatment for these infections. the purpose of our study was to sequence and characterize three novel bcc-specific phages: ks5 (vb_bcem-ks5 or vb_bmuz-atcc 17616), ks14 (vb_bcem-ks14) and kl3 (vb_bamm-kl3 or vb_bcez-cep511). | 2010 | 20973964 |
| characterization of filamentous bacteriophage pe226 infecting ralstonia solanacearum strains. | the aim of this study was to isolate and characterize new bacteriophages that infect a wide range of plant pathogenic ralstonia solanacearum strains. | 2010 | 21054700 |
| development of real-time pcr assays for detection and quantification of bacillus cereus group species: differentiation of b. weihenstephanensis and rhizoid b. pseudomycoides isolates from milk. | quantitative real-time pcr (qrt-pcr) offers an alternative method for the detection of bacterial contamination in food. this method provides the quantitation and determination of the number of gene copies. in our study, we established an rt-pcr assay using the lightcycler system to detect and quantify the bacillus cereus group species, which includes b. cereus, b. anthracis, b. thuringiensis, b. weihenstephanensis, b. mycoides, and b. pseudomycoides. a taqman assay was designed to detect a 285-b ... | 2010 | 21057027 |
| development of real-time pcr assays for detection and quantification of bacillus cereus group species: differentiation of b. weihenstephanensis and rhizoid b. pseudomycoides isolates from milk. | quantitative real-time pcr (qrt-pcr) offers an alternative method for the detection of bacterial contamination in food. this method provides the quantitation and determination of the number of gene copies. in our study, we established an rt-pcr assay using the lightcycler system to detect and quantify the bacillus cereus group species, which includes b. cereus, b. anthracis, b. thuringiensis, b. weihenstephanensis, b. mycoides, and b. pseudomycoides. a taqman assay was designed to detect a 285-b ... | 2010 | 21057027 |
| aerobic degradation of mercaptosuccinate by the gram-negative bacterium variovorax paradoxus strain b4. | the gram-negative bacterium variovorax paradoxus strain b4 was isolated from soil under mesophilic and aerobic conditions to elucidate the so far unknown catabolism of mercaptosuccinate (ms). during growth with ms this strain released significant amounts of sulfate into the medium. tn5::mob-induced mutagenesis was successfully employed and yielded nine independent mutants incapable of using ms as a carbon source. in six of these mutants, tn5::mob insertions were mapped in a putative gene encodin ... | 2010 | 21075928 |
| aerobic degradation of mercaptosuccinate by the gram-negative bacterium variovorax paradoxus strain b4. | the gram-negative bacterium variovorax paradoxus strain b4 was isolated from soil under mesophilic and aerobic conditions to elucidate the so far unknown catabolism of mercaptosuccinate (ms). during growth with ms this strain released significant amounts of sulfate into the medium. tn5::mob-induced mutagenesis was successfully employed and yielded nine independent mutants incapable of using ms as a carbon source. in six of these mutants, tn5::mob insertions were mapped in a putative gene encodin ... | 2010 | 21075928 |
| ralstonia pickettii community-acquired pneumonia in cambodia. | | 2010 | 21144257 |
| novel pan-genomic analysis approach in target selection for multiplex pcr identification and detection of burkholderia pseudomallei, burkholderia thailandensis, and burkholderia cepacia complex species: a proof-of-concept study. | burkholderia pseudomallei, burkholderia thailandensis, and the burkholderia cepacia complex differ greatly in pathogenicity and epidemiology. yet, they are occasionally misidentified by biochemical profiling, and even 16s rrna gene sequencing may not offer adequate discrimination between certain species groups. using the 23 b. pseudomallei, four b. thailandensis, and 16 b. cepacia complex genome sequences available, we identified gene targets specific to each of them (a tat domain protein, a 70- ... | 2010 | 21177905 |
| rapid identification of gram-negative bacteria with and without ctx-m extended-spectrum ß-lactamase from positive blood culture bottles by pcr followed by microchip gel electrophoresis. | we evaluated the usefulness of pcr analysis of the 16s-23s rrna gene internal transcribed spacer (its) and the ctx-m extended-spectrum ß-lactamase (esbl) followed by microchip gel electrophoresis (mge) for direct identification and ctx-m detection of gram-negative bacteria (gnb) from positive blood culture bottles. of 251 gnb isolated from blood cultures containing a single bacterium, 225 (90%) were correctly identified at the species level directly from positive blood culture bottles by compari ... | 2011 | 21289149 |
| gradually accumulating beneficial mutations to improve the thermostability of n-carbamoyl-d-amino acid amidohydrolase by step-wise evolution. | to further enhance repeated batch reactions with immobilized n-carbamoyl-d-amino acid amidohydrolase (dcase), which can be used for the industrial production of d-amino acids, the stability of high soluble mutant dcase-m3 from ralstonia pickettii cgmcc1596 was improved by step-wise evolution. in our previous report, six thermostability-related sites were identified by error-prone pcr. based on the above result, an improved mutant b5 (q12l/q23l/h248q/t262a/t263s) was obtained through two rounds o ... | 2011 | 21360152 |
| characterisation of the putative effector interaction site of the regulatory hbpr protein from pseudomonas azelaica by site-directed mutagenesis. | bacterial transcription activators of the xylr/dmpr subfamily exert their expression control via ¤â(54)-dependent rna polymerase upon stimulation by a chemical effector, typically an aromatic compound. where the chemical effector interacts with the transcription regulator protein to achieve activation is still largely unknown. here we focus on the hbpr protein from pseudomonas azelaica, which is a member of the xylr/dmpr subfamily and responds to biaromatic effectors such as 2-hydroxybiphenyl. w ... | 2011 | 21379585 |
| biodiversity and geochemistry of an extremely acidic, low-temperature subterranean environment sustained by chemolithotrophy. | the geochemical dynamics and composition of microbial communities within a low-temperature (ôê+8.5-¦c), long-abandoned (>ôçâ90 years) underground pyrite mine (cae coch, located in north wales) were investigated. surface water percolating through fractures in the residual pyrite ore body that forms the roof of the mine becomes extremely acidic and iron-enriched due to microbially accelerated oxidative dissolution of the sulfide mineral. water droplets on the mine roof were found to host a very li ... | 2011 | 21382147 |
| a motn mutant of ralstonia solanacearum is hypermotile and has reduced virulence. | ralstonia solanacearum is a soil-borne plant pathogen that causes bacterial wilt disease on many plant species. we previously showed that swimming motility contributes to virulence of this bacterium in the early stages of host invasion and colonization. in this study we identified a new negative regulator of motility, named motn, that is located in a cluster of motility-related genes. a motn mutant was hypermotile both on 0.3% agar motility plates and in rich and minimal medium broth. however, l ... | 2011 | 21421761 |
| differentiating the growing nosocomial infectious threats ralstonia pickettii and ralstonia insidiosa. | differentiation of the growing nosocomial infectious threats, ralstonia pickettii and ralstonia insidiosa, based on nitrate reduction, desferrioxamine susceptibility, arabinose, n-acetyl-glucosamine and phenylacetate assimilation is described. these tests can be used for preliminary identification of ralstonia pickettii and ralstonia insidiosa resulting in more accurate identification of these species. | 2011 | 21461848 |
| microbiology of animal bite wound infections. | the microbiology of animal bite wound infections in humans is often polymicrobial, with a broad mixture of aerobic and anaerobic microorganisms. bacteria recovered from infected bite wounds are most often reflective of the oral flora of the biting animal, which can also be influenced by the microbiome of their ingested prey and other foods. bacteria may also originate from the victim's own skin or the physical environment at the time of injury. our review has focused on bite wound infections in ... | 2011 | 21482724 |
| degradation of chlorobenzene by strain ralstonia pickettii l2 isolated from a biotrickling filter treating a chlorobenzene-contaminated gas stream. | a ralstonia pickettii species able to degrade chlorobenzene (cb) as the sole source of carbon and energy was isolated from a biotrickling filter used for the removal of cb from waste gases. this organism, strain l2, could degrade cb as high as 220 mg/l completely. following cb consumption, stoichiometric amounts of chloride were released, and co(2) production rate up to 80.2% proved that the loss of cb was mainly via mineralization and incorporation into cell material. the haldane modification o ... | 2011 | 21499764 |
| bioinformatic characterization of the trimeric intracellular cation-specific channel protein family. | trimeric intracellular cation-specific (tric) channels are integral to muscle excitation-contraction coupling. tric channels provide counter-ionic flux when calcium is rapidly transported from intracellular stores to the cell cytoplasm. until recently, knowledge of the presence of these proteins was limited to animals. we analyzed the tric family and identified a profusion of prokaryotic family members with topologies and motifs similar to those of their eukaryotic counterparts. prokaryotic memb ... | 2011 | 21519847 |
| classification of plant associated bacteria using rif, a computationally derived dna marker. | a dna marker that distinguishes plant associated bacteria at the species level and below was derived by comparing six sequenced genomes of xanthomonas, a genus that contains many important phytopathogens. this dna marker comprises a portion of the dnaa replication initiation factor (rif). unlike the rrna genes, dnaa is a single copy gene in the vast majority of sequenced bacterial genomes, and amplification of rif requires genus-specific primers. in silico analysis revealed that rif has equal or ... | 2011 | 21533033 |
| surveillance of single-cell behavior in different subpopulations of ralstonia pickettii ar1 during growth and polyhydroxybutyrate production phases by flow cytometry. | most bacterial strains accumulate intracellular polyhydroxybutyrate (phb) granules as an energy reservoir, in response to fluctuations in their microenvironment. flow cytometry was applied for the analysis of single cells of ralstonia pickettii ar1 in response to changes in the culture conditions. two parameters, the phb production-related fl2 and side scatter (ssc) parameters, were used to monitor, distinguish and characterize different subpopulations in the growth and phb production phases. a ... | 2011 | 21780145 |
| environmental adaptability and stress tolerance of laribacter hongkongensis: a genome-wide analysis. | | 2011 | 21711489 |
| mechanisms and evolution of oxidative sulfur metabolism in green sulfur bacteria. | green sulfur bacteria (gsb) constitute a closely related group of photoautotrophic and thiotrophic bacteria with limited phenotypic variation. they typically oxidize sulfide and thiosulfate to sulfate with sulfur globules as an intermediate. based on genome sequence information from 15 strains, the distribution and phylogeny of enzymes involved in their oxidative sulfur metabolism was investigated. at least one homolog of sulfide:quinone oxidoreductase (sqr) is present in all strains. in all sul ... | 2011 | 21833341 |
| lobar pneumonia caused by ralstonia pickettii in a sixty-five-year-old han chinese man: a case report. | abstract: | 2011 | 21843320 |
| emerging roles for the ro 60-kda autoantigen in noncoding rna metabolism. | all cells contain an enormous variety of ribonucleoprotein (rnp) complexes that function in diverse processes. although the mechanisms by which many of these rnps contribute to cell metabolism are well understood, the roles of others are only now beginning to be revealed. a member of this latter category, the ro 60-kda protein and its associated noncoding y rnas, was discovered because the protein component is a frequent target of the autoimmune response in patients with the rheumatic diseases s ... | 2011 | 21823229 |
| antibacterial activity of some selected medicinal plants of pakistan. | screening of the ethnobotenical plants is a pre-requisite to evaluate their therapeutic potential and it can lead to the isolation of new bioactive compounds. | 2011 | 21718504 |